Grade 9 second-language learners in township schools : issues of language and mathematics when solving word problems
- Authors: Sepeng, Johannes Percy
- Date: 2010
- Subjects: Mathematics -- Language , Mathematics -- Study and teaching , Language and languages -- Mathematics , Second language acquisition -- Study and teaching
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9498 , http://hdl.handle.net/10948/1455 , Mathematics -- Language , Mathematics -- Study and teaching , Language and languages -- Mathematics , Second language acquisition -- Study and teaching
- Description: Second language (English) learning of mathematics is common in South African mathematics classrooms, including those in the Eastern Cape Province of South Africa where isiXhosa speakers are taught in the language that is not spoken at home by both teachers and learners. The purpose of this research was to investigate issues of language, both home (isiXhosa) and the language of learning and teaching (LoLT), i.e. English, when 9th grade second language learners engage in problem-solving and sense-making of wor(l)d problems in multilingual mathematics classrooms. In addition, the aim of the study was to explore whether the introduction of discussion and argumentation techniques in these classrooms can ameliorate these issues. The study used a pre-test – intervention – post-test mixed method design utilising both quantitative and qualitative data. The data collection strategies for the purpose of this study included interviews (learners [n=24] and teachers [n=4]), classroom observations, and tests (experimental [n=107] and comparison [69]) in four experimental and two comparison schools in townships of Port Elizabeth. This study is framed by socio-cultural perspective which proposes that collective and individual processes are directly related and that students‘ unrealistic responses to real world problems reflect the students‘ socio-cultural relationship to school mathematics and their willingness to employ the approaches emphasised in school. Analysis of the data generated from pre- and post-tests, interviews and classroom observation schedule suggest that the interventional strategy significantly improved the experimental learners‘ problem-solving skills and sense-making abilities in both English and isiXhosa (but more significantly in English). The statistical results illustrate that the experimental group performed statistical significantly (p < .0005) better in the English posttest compared to comparison group. The data also suggests that the interventional strategy in this study (discussion and argumentation techniques) positively influenced the participating learners‘ word problem-solving abilities. The experimental group appeared to show a tendency to consider reality marginally better than the comparison group after the intervention. In particular, learners seemed to make realistic considerations better in the isiXhosa translation post-test compared to the English post-test. A large practical significant (d = 0.86) difference between the experimental group and the comparison group was also noted in the isiXhosa translation compared to a moderate practical significance (d = 0.57) noted in the English tests after the intervention. As such, the results of the study suggest that the introduction of discussion and argumentation techniques in the teaching and learning of mathematics word problems had a positive effect on learners‘ ability to consider reality during word problem-solving in both languages. Analysis of learners‘ interviews suggests that, although English is the preferred LoLT, they would prefer dual-use/parallel-use of English and isiXhosa for teaching and learning mathematics. There was also evidence of the benefits of code-switching throughout most of the lessons observed, coupled with instances of peer translation, and/or re-voicing. Overall results in this study illustrate that number skills displayed and mathematical errors made by learners seem to be directly related to language use in the classroom.
- Full Text:
- Date Issued: 2010
- Authors: Sepeng, Johannes Percy
- Date: 2010
- Subjects: Mathematics -- Language , Mathematics -- Study and teaching , Language and languages -- Mathematics , Second language acquisition -- Study and teaching
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9498 , http://hdl.handle.net/10948/1455 , Mathematics -- Language , Mathematics -- Study and teaching , Language and languages -- Mathematics , Second language acquisition -- Study and teaching
- Description: Second language (English) learning of mathematics is common in South African mathematics classrooms, including those in the Eastern Cape Province of South Africa where isiXhosa speakers are taught in the language that is not spoken at home by both teachers and learners. The purpose of this research was to investigate issues of language, both home (isiXhosa) and the language of learning and teaching (LoLT), i.e. English, when 9th grade second language learners engage in problem-solving and sense-making of wor(l)d problems in multilingual mathematics classrooms. In addition, the aim of the study was to explore whether the introduction of discussion and argumentation techniques in these classrooms can ameliorate these issues. The study used a pre-test – intervention – post-test mixed method design utilising both quantitative and qualitative data. The data collection strategies for the purpose of this study included interviews (learners [n=24] and teachers [n=4]), classroom observations, and tests (experimental [n=107] and comparison [69]) in four experimental and two comparison schools in townships of Port Elizabeth. This study is framed by socio-cultural perspective which proposes that collective and individual processes are directly related and that students‘ unrealistic responses to real world problems reflect the students‘ socio-cultural relationship to school mathematics and their willingness to employ the approaches emphasised in school. Analysis of the data generated from pre- and post-tests, interviews and classroom observation schedule suggest that the interventional strategy significantly improved the experimental learners‘ problem-solving skills and sense-making abilities in both English and isiXhosa (but more significantly in English). The statistical results illustrate that the experimental group performed statistical significantly (p < .0005) better in the English posttest compared to comparison group. The data also suggests that the interventional strategy in this study (discussion and argumentation techniques) positively influenced the participating learners‘ word problem-solving abilities. The experimental group appeared to show a tendency to consider reality marginally better than the comparison group after the intervention. In particular, learners seemed to make realistic considerations better in the isiXhosa translation post-test compared to the English post-test. A large practical significant (d = 0.86) difference between the experimental group and the comparison group was also noted in the isiXhosa translation compared to a moderate practical significance (d = 0.57) noted in the English tests after the intervention. As such, the results of the study suggest that the introduction of discussion and argumentation techniques in the teaching and learning of mathematics word problems had a positive effect on learners‘ ability to consider reality during word problem-solving in both languages. Analysis of learners‘ interviews suggests that, although English is the preferred LoLT, they would prefer dual-use/parallel-use of English and isiXhosa for teaching and learning mathematics. There was also evidence of the benefits of code-switching throughout most of the lessons observed, coupled with instances of peer translation, and/or re-voicing. Overall results in this study illustrate that number skills displayed and mathematical errors made by learners seem to be directly related to language use in the classroom.
- Full Text:
- Date Issued: 2010
Historical biogeography of the tribe Platypleurini Schmidt, 1918 (Hemiptera: Cicadidae) with a focus on Southern Africa
- Authors: Price, Benjamin Wills
- Date: 2010
- Subjects: Biogeography -- History -- Africa, Southern Cicadas -- Africa, Southern Cicada (Genus) -- Africa, Southern Biodiversity -- Africa, Southern Cicadas -- Variation -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5708 , http://hdl.handle.net/10962/d1005394
- Description: With our contemporary biota under increasing threat of extinction, it is of interest to understand where, why and how biological diversity is generated. If focussed on appropriate taxa, phylogeographic and phylogenetic studies can assist in the identification of both places and processes central to the origin and maintenance of biological diversity. It is explained why southern Africa presents a perfect test-bed for exploring such mechanisms of diversification and why cicadas (Hemiptera: Cicadidae) have proved very suitable tools for studies of historical biogeography. This study then exemplifies these points by providing the first large-scale investigation of the historical biogeography of the tribe Platypleurini Schmidt, 1918, with emphasis on the genus Platypleura Amyot & Seville, 1843 in southern Africa. Standard methods of DNA sequencing provided data from portions of the mitochondrial small subunit ribosomal 16S RNA (16S) and cytochrome oxidase subunits I (COI) and II (COII); and the nuclear elongation factor 1 alpha (EF-1α) from 400 ethanol-preserved specimens. These data were analysed using standard phylogenetic methods and a time scale of diversification was estimated using a Bayesian framework and both fossil data and DNA substitution rates. The results showed that the tribe is too recent to be of Gondwanan origin. The lack of monophyly of the genera represented in both Asia and Africa showed that the tribe needs formal taxonomic revision. Diversification of the African platypleurine genera coincides with aridification in the early Oligocene. Dispersal of Asian platypleurine taxa coincides with the meeting of Africa and Eurasia in the mid-Oligocene. Two radiations within African Platypleura are hypothesised; one distributed over most of sub-Saharan Africa and the second restricted to southern Africa, with clades restricted within regional biomes. Within each of the three focal biomes, cryptic taxonomic diversity was confirmed, suggesting that, even in relatively well understood groups such as the southern African platypleurine cicadas, molecular data can identify further diversity. Although each focal taxon was restricted to non-overlapping biomes, comparison of the three biomes highlighted interactions between palaeoclimates and fixed landscape features (coastal topography, river catchments and escarpments) as causative agents of vicariance, dispersal, extinction and diversification of these volant insects. The results of using co-distributed species for comparative study cautions against making inferences based on single-taxon datasets and highlights the need to use many, evolutionarily independent taxa when identifying mechanisms of diversification. The dating analyses imply that within-species lineage diversification occurred overwhelmingly within the Pleistocene, a trend that is being increasingly recognised in print for other biota. Some caveats about using phylogenetic approaches to estimate ancestral areas are illustrated. Several recommendations are made regarding additional taxa and data sources for understanding the origin and maintenance of biological diversity.
- Full Text:
- Date Issued: 2010
- Authors: Price, Benjamin Wills
- Date: 2010
- Subjects: Biogeography -- History -- Africa, Southern Cicadas -- Africa, Southern Cicada (Genus) -- Africa, Southern Biodiversity -- Africa, Southern Cicadas -- Variation -- Africa, Southern
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5708 , http://hdl.handle.net/10962/d1005394
- Description: With our contemporary biota under increasing threat of extinction, it is of interest to understand where, why and how biological diversity is generated. If focussed on appropriate taxa, phylogeographic and phylogenetic studies can assist in the identification of both places and processes central to the origin and maintenance of biological diversity. It is explained why southern Africa presents a perfect test-bed for exploring such mechanisms of diversification and why cicadas (Hemiptera: Cicadidae) have proved very suitable tools for studies of historical biogeography. This study then exemplifies these points by providing the first large-scale investigation of the historical biogeography of the tribe Platypleurini Schmidt, 1918, with emphasis on the genus Platypleura Amyot & Seville, 1843 in southern Africa. Standard methods of DNA sequencing provided data from portions of the mitochondrial small subunit ribosomal 16S RNA (16S) and cytochrome oxidase subunits I (COI) and II (COII); and the nuclear elongation factor 1 alpha (EF-1α) from 400 ethanol-preserved specimens. These data were analysed using standard phylogenetic methods and a time scale of diversification was estimated using a Bayesian framework and both fossil data and DNA substitution rates. The results showed that the tribe is too recent to be of Gondwanan origin. The lack of monophyly of the genera represented in both Asia and Africa showed that the tribe needs formal taxonomic revision. Diversification of the African platypleurine genera coincides with aridification in the early Oligocene. Dispersal of Asian platypleurine taxa coincides with the meeting of Africa and Eurasia in the mid-Oligocene. Two radiations within African Platypleura are hypothesised; one distributed over most of sub-Saharan Africa and the second restricted to southern Africa, with clades restricted within regional biomes. Within each of the three focal biomes, cryptic taxonomic diversity was confirmed, suggesting that, even in relatively well understood groups such as the southern African platypleurine cicadas, molecular data can identify further diversity. Although each focal taxon was restricted to non-overlapping biomes, comparison of the three biomes highlighted interactions between palaeoclimates and fixed landscape features (coastal topography, river catchments and escarpments) as causative agents of vicariance, dispersal, extinction and diversification of these volant insects. The results of using co-distributed species for comparative study cautions against making inferences based on single-taxon datasets and highlights the need to use many, evolutionarily independent taxa when identifying mechanisms of diversification. The dating analyses imply that within-species lineage diversification occurred overwhelmingly within the Pleistocene, a trend that is being increasingly recognised in print for other biota. Some caveats about using phylogenetic approaches to estimate ancestral areas are illustrated. Several recommendations are made regarding additional taxa and data sources for understanding the origin and maintenance of biological diversity.
- Full Text:
- Date Issued: 2010
Hotel rating through guest feedback
- Authors: Hensens, Wouter
- Date: 2010
- Subjects: Hospitality industry -- Customer services , Hotels , Hotels -- Evaluation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9264 , http://hdl.handle.net/10948/1631 , Hospitality industry -- Customer services , Hotels , Hotels -- Evaluation
- Description: Hotel rating refers to the process where the comfort and services of a hotel are assessed and classified, usually in five categories, using stars as symbols. Conventional hotel rating systems are generally operated by governments or independent parties. However, with the growth of social media and customer-review sites, guest review platforms became an important source of information. The main aim of this study is to establish whether guest feedback can determine hotel ratings more accurately than conventional methods and whether a social media platform such as TripAdvisor can provide the necessary data to do so. The customer-review website, TripAdvisor, has grown rapidly and made a strong impact on the tourism and hotel industry. This study identifies the nature of TripAdvisor, its reliability, how its ratings compare with conventional ratings, and what criteria are used in guest reviews on TripAdvisor when assessing the quality of a hotel. These findings were triangulated with findings from the conventional rating systems of the 11 destinations that were sampled for this study to identify the value of TripAdvisor. Two samples were taken from TripAdvisor of 110 and 33 hotels, respectively. From the latter, ten guest reviews were gathered and analysed per hotel, resulting in a total of 330 reviews that were analysed. The study’s findings indicate that TripAdvisor is the largest guest feedback platform for hotels and its data can be considered to be reliable. The TripAdvisor ratings were not connected to the conventional ratings of the sampled hotels. The criteria used in TripAdvisor reviews focused more on service delivery than on the objective tangible elements used in most hotel rating systems. The rich context found in most guest reviews makes the information presented on TripAdvisor valuable. There is no evidence that conventionalrating system controls are linked to the comments found in TripAdvisor reviews. The results facilitated the identification of the delight and frustration factors in services marketing for the hotel industry. A new theory to include guest feedback in hotel ratings is developed and proposed. The study further presents two future scenarios, the most likely one of which predicts the demise of conventional rating systems as a result of the success of guest feedback platforms such as TripAdvisor.
- Full Text:
- Date Issued: 2010
- Authors: Hensens, Wouter
- Date: 2010
- Subjects: Hospitality industry -- Customer services , Hotels , Hotels -- Evaluation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9264 , http://hdl.handle.net/10948/1631 , Hospitality industry -- Customer services , Hotels , Hotels -- Evaluation
- Description: Hotel rating refers to the process where the comfort and services of a hotel are assessed and classified, usually in five categories, using stars as symbols. Conventional hotel rating systems are generally operated by governments or independent parties. However, with the growth of social media and customer-review sites, guest review platforms became an important source of information. The main aim of this study is to establish whether guest feedback can determine hotel ratings more accurately than conventional methods and whether a social media platform such as TripAdvisor can provide the necessary data to do so. The customer-review website, TripAdvisor, has grown rapidly and made a strong impact on the tourism and hotel industry. This study identifies the nature of TripAdvisor, its reliability, how its ratings compare with conventional ratings, and what criteria are used in guest reviews on TripAdvisor when assessing the quality of a hotel. These findings were triangulated with findings from the conventional rating systems of the 11 destinations that were sampled for this study to identify the value of TripAdvisor. Two samples were taken from TripAdvisor of 110 and 33 hotels, respectively. From the latter, ten guest reviews were gathered and analysed per hotel, resulting in a total of 330 reviews that were analysed. The study’s findings indicate that TripAdvisor is the largest guest feedback platform for hotels and its data can be considered to be reliable. The TripAdvisor ratings were not connected to the conventional ratings of the sampled hotels. The criteria used in TripAdvisor reviews focused more on service delivery than on the objective tangible elements used in most hotel rating systems. The rich context found in most guest reviews makes the information presented on TripAdvisor valuable. There is no evidence that conventionalrating system controls are linked to the comments found in TripAdvisor reviews. The results facilitated the identification of the delight and frustration factors in services marketing for the hotel industry. A new theory to include guest feedback in hotel ratings is developed and proposed. The study further presents two future scenarios, the most likely one of which predicts the demise of conventional rating systems as a result of the success of guest feedback platforms such as TripAdvisor.
- Full Text:
- Date Issued: 2010
Implementation of novel flow cytometric methods to assess the in vitro antidiabetic mechanism of a Sutherlandia Frutescens extract
- Authors: Elliot, Gayle Pamela
- Date: 2010
- Subjects: Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10304 , http://hdl.handle.net/10948/1439 , Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Description: The ability of insulin to stimulate glucose uptake into muscle and adipose tissue is central to the maintenance of whole-body glucose homeostasis. Deregulation of insulin action manifests itself as insulin resistance, a key component of type 2 diabetes. Insulin resistance is also observed in HIV patients receiving protease inhibitors. An agent that can reversibly induce an insulin-resistant state would be a very useful tool in developing model systems that mimic the pathogenesis of type 2 diabetes. Insulin resistance can arise from defects in insulin signal transduction, changes in the expression of proteins or genes that are targets of insulin action, cross talk from other hormonal systems or metabolic abnormalities. Deterioration of the insulin-receptor-signalling pathway at different levels leading to decreased levels of signalling pathway intermediates and/or decreased activation through phosphorylation accounts for the evolution from an insulin-resistant state to type 2 diabetes. In addition, defects in GLUT4 glucose transporter translocation are observed, further fuelling impairments in skeletal muscle glucose uptake. Levels of insulin-induced GLUT4 translocation in the skeletal muscle of type 2 diabetic patients are typically reduced by 90%. Many cellular pathways & their intermediates are in some way or another linked to insulin signalling. This study focused on three of these namely the PI3-kinase/Akt pathway, the Mitogen Activated Protein Kinase (MAPK) cascade and the AMP Kinase pathway, with successful monitoring of the PI3-K pathway. Investigations involved observing and evaluating the effects of various compounds as well as an indigenous medicinal plant, Sutherlandia frutescens on the activities of key insulin signalling pathway intermediates within the three fore mentioned pathways including Akt, AMPK and MEK1/2 as well as membrane surface GLUT4 levels. Scientific research has in the past leant heavily on Western blotting as the method of choice for gaining vital information relating to signal transduction pathways, however for research into cellular mechanisms the negatives of this method outweigh the positives. The drawbacks include a need for large amount of cells, multiple washing steps which may be disadvantageous to any weak and transient interactions as well as lysing of cells which may interfere with the maintenance of the subcellular localisation of a specific signalling event. Based on these, the need for a better method in terms of speed & reliability to monitor phosphorylation states of signal transduction pathway intermediates & GLUT4 translocation was evident and was one VII of the main aims & successes of this study. The method created used the mouse muscle cell line C2C12 in conjunction with the quick, sensitive method of flow cytometry which allowed us to monitor these processes in these cells through immune-labelling. Adherent cell cultures such as the C2C12 cell line pose the problem of possible damage to plasma membrane receptors (including insulin receptors) during harvesting to obtain a cell suspension for flow cytometry. We however used C2C12 mouse myocytes to optimize a method yielding insulin responsive cells in suspension that were successfully used for flow cytometry after immunelabelling of insulin signalling intermediates. Insulin (0.1μM) significantly raised the levels of both P-Akt and GLUT4 above basal levels. This effect was shown to be dose dependent. At a concentration of 50μg/ml, Sutherlandia frutescens was able to act as an insulin-mimetic in terms of its ability to increase P-Akt levels, GLUT4 translocation and glucose utilisation in an acute manner. These increases could be reduced with the addition of wortmannin, a PI3-K inhibitor. Therefore, these results suggest the mechanism of the plant extract’s insulin-like activity may be in part due to the activation of the insulin signalling pathway leading to GLUT4 translocation, which involves the phosphorylation of insulin receptor- and subsequent PI3-K activity, leading to P-Akt activity. These results provide further evidence of this plant extract’s anti-diabetic potential. The effect of Sutherlandia frutescens on insulin secretion, calcium signalling and proliferation in INS-1 rat pancreatic cells was also investigated and it was found to increase the activities of all of these processes. However no change in the levels of GLUT2 glucose transporter was seen. Ritonavir is prescribed by the South African Department of Health in co-formulation with other protease inhibitors within its second regime in the treatment of HIV and AIDS. Using C2C12 cells, ritonavir decreased glucose uptake acutely and had no effect on GLUT4 translocation however surprisingly increased P-Akt levels. In conclusion, it was found that Sutherlandia frutescens has antidiabetic benefits, diverse in nature depending on tissue type as well as length of time administered. The establishment of novel flow cytometry techniques to assess antidiabetic properties using in vitro cell culture was achieved. These methods will be useful in the future for the assessment of insulin sensitivity and in the identification of novel compounds that stimulate the insulin signalling pathways.
- Full Text:
- Date Issued: 2010
- Authors: Elliot, Gayle Pamela
- Date: 2010
- Subjects: Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10304 , http://hdl.handle.net/10948/1439 , Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Description: The ability of insulin to stimulate glucose uptake into muscle and adipose tissue is central to the maintenance of whole-body glucose homeostasis. Deregulation of insulin action manifests itself as insulin resistance, a key component of type 2 diabetes. Insulin resistance is also observed in HIV patients receiving protease inhibitors. An agent that can reversibly induce an insulin-resistant state would be a very useful tool in developing model systems that mimic the pathogenesis of type 2 diabetes. Insulin resistance can arise from defects in insulin signal transduction, changes in the expression of proteins or genes that are targets of insulin action, cross talk from other hormonal systems or metabolic abnormalities. Deterioration of the insulin-receptor-signalling pathway at different levels leading to decreased levels of signalling pathway intermediates and/or decreased activation through phosphorylation accounts for the evolution from an insulin-resistant state to type 2 diabetes. In addition, defects in GLUT4 glucose transporter translocation are observed, further fuelling impairments in skeletal muscle glucose uptake. Levels of insulin-induced GLUT4 translocation in the skeletal muscle of type 2 diabetic patients are typically reduced by 90%. Many cellular pathways & their intermediates are in some way or another linked to insulin signalling. This study focused on three of these namely the PI3-kinase/Akt pathway, the Mitogen Activated Protein Kinase (MAPK) cascade and the AMP Kinase pathway, with successful monitoring of the PI3-K pathway. Investigations involved observing and evaluating the effects of various compounds as well as an indigenous medicinal plant, Sutherlandia frutescens on the activities of key insulin signalling pathway intermediates within the three fore mentioned pathways including Akt, AMPK and MEK1/2 as well as membrane surface GLUT4 levels. Scientific research has in the past leant heavily on Western blotting as the method of choice for gaining vital information relating to signal transduction pathways, however for research into cellular mechanisms the negatives of this method outweigh the positives. The drawbacks include a need for large amount of cells, multiple washing steps which may be disadvantageous to any weak and transient interactions as well as lysing of cells which may interfere with the maintenance of the subcellular localisation of a specific signalling event. Based on these, the need for a better method in terms of speed & reliability to monitor phosphorylation states of signal transduction pathway intermediates & GLUT4 translocation was evident and was one VII of the main aims & successes of this study. The method created used the mouse muscle cell line C2C12 in conjunction with the quick, sensitive method of flow cytometry which allowed us to monitor these processes in these cells through immune-labelling. Adherent cell cultures such as the C2C12 cell line pose the problem of possible damage to plasma membrane receptors (including insulin receptors) during harvesting to obtain a cell suspension for flow cytometry. We however used C2C12 mouse myocytes to optimize a method yielding insulin responsive cells in suspension that were successfully used for flow cytometry after immunelabelling of insulin signalling intermediates. Insulin (0.1μM) significantly raised the levels of both P-Akt and GLUT4 above basal levels. This effect was shown to be dose dependent. At a concentration of 50μg/ml, Sutherlandia frutescens was able to act as an insulin-mimetic in terms of its ability to increase P-Akt levels, GLUT4 translocation and glucose utilisation in an acute manner. These increases could be reduced with the addition of wortmannin, a PI3-K inhibitor. Therefore, these results suggest the mechanism of the plant extract’s insulin-like activity may be in part due to the activation of the insulin signalling pathway leading to GLUT4 translocation, which involves the phosphorylation of insulin receptor- and subsequent PI3-K activity, leading to P-Akt activity. These results provide further evidence of this plant extract’s anti-diabetic potential. The effect of Sutherlandia frutescens on insulin secretion, calcium signalling and proliferation in INS-1 rat pancreatic cells was also investigated and it was found to increase the activities of all of these processes. However no change in the levels of GLUT2 glucose transporter was seen. Ritonavir is prescribed by the South African Department of Health in co-formulation with other protease inhibitors within its second regime in the treatment of HIV and AIDS. Using C2C12 cells, ritonavir decreased glucose uptake acutely and had no effect on GLUT4 translocation however surprisingly increased P-Akt levels. In conclusion, it was found that Sutherlandia frutescens has antidiabetic benefits, diverse in nature depending on tissue type as well as length of time administered. The establishment of novel flow cytometry techniques to assess antidiabetic properties using in vitro cell culture was achieved. These methods will be useful in the future for the assessment of insulin sensitivity and in the identification of novel compounds that stimulate the insulin signalling pathways.
- Full Text:
- Date Issued: 2010
Improving Productivity of Maize-based Smallholder Irrigated Cropping Systems: A Case Study of Zanyokwe Irrigation Scheme, Eastern Cape, South Africa
- Authors: Fanadzo, Morris
- Date: 2010
- Subjects: Farms, Small -- South Africa -- Eastern Cape Crop yields -- South Africa -- Eastern Cape Agricultural productivity -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/11009 , vital:36450
- Description: Smallholder irrigation schemes (SIS) in South Africa have performed poorly and failed to achieve the development objectives of sustaining rural livelihoods by increasing crop production and income. Limited knowledge of crop production among farmers is one constraint to improved crop productivity and, yet little research has been carried out to relate agronomic practices to productivity. This study was aimed at identifying strategies to improve the productivity of maize-based smallholder irrigated cropping systems in South Africa, using Zanyokwe irrigation scheme (ZIS) in the Eastern Cape Province as a case study. The research design was emergent, developed progressively as influenced by the progressive analyses that were made. The research work involved two main phases: exploratory studies and refinement on-farm experiments for testing technologies as informed by the exploratory studies. Exploratory studies were aimed at describing farmer crop production practices in major crop enterprises and identifying and prioritizing major agronomic yield constraints. Findings indicated that the overall performance of the cropping systems was poor as evidenced by low overall yields of < 25% of the potential in maize and butternut. Poor management of weeds, soil fertility, plant populations and water, late planting and poor cultivar choice were the most important yield constraints. The average cropping intensity achieved by farmers was only 48%. New hybrids yielded 50 - 65% higher grain than the cultivars commonly grown by farmers. However, farmers favoured their traditional cultivars, as they preferred green maize production as opposed to dry grains. As a result of these findings, focused research was designed on options for iii improving green maize production, soil fertility, crop stand and weed management in crop production. On-farm trials were, therefore, designed to (1) compare the response to nitrogen (N) rate of direct seeded and transplanted maize; (2) evaluate comparative performance of direct seeded and transplanted green maize under farmer management, and to work out the economics of transplanting; (3) investigate the effects of row spacing and atrazine dosage on weed dynamics maize yield; (4) determine the effects of inter-row spacing and plant population on weed dynamics and maize yield; and (5) investigate the effects of pre-plant weed control, N rate and plant population on weed dynamics and butternut yield. Maize transplanting improved crop stand, had earlier maturity and yielded higher at lower N rates compared to direct seeding for both green and grain maize. Total costs that varied were higher by R3 565 ha-1 for transplanted maize compared to direct-seeded maize, but the net benefits from transplanting were also much higher by R8 773 ha-1. Farmers favoured transplanting, citing bigger cobs, early maturity and the absence of seedling damage by birds, when maize was transplanted. However, a follow-up survey conducted during the subsequent year indicated that five of the six farmers abandoned transplanting, citing shortage of labour and high cost of commercially produced seedlings. It was concluded that transplanting is unlikely to be adopted unless the labour intensiveness of manual transplanting can be solved. Increasing atrazine dosage from a third of the label recommended dosage (LRD) and reducing row spacing from 90 to 45 cm resulted in an increase in weed mortality. A 100% kill of annual broadleaf weeds was possible while iv perennial grasses and the annual broadleaf weed, Oxalis latifolia, could not be controlled even at the LRD. However, improved weed mortality with increase in atrazine dosage and/or narrow rows did not translate into improved maize productivity. In butternut, pre-plant weed control resulted in a six-fold decrease in weed biomass and a 100% increase in fruit yield. These findings demonstrated the possibility of incorporation of reduced herbicide dosages and narrow rows to achieve adequate weed control and optimise on maize yields in smallholder farming systems. The results also suggested that pre-plant weed control is one of the pre-requisite to successful butternut production in SIS in South Africa. Increasing maize population from 40 000 to 60 000 plants ha-1 resulted in a 30% grain yield increase, but decreased length of cobs. Row spacing had no effect on maize yield at 40 000 plants ha-1, but at 60 000 plants ha-1 45 cm rows resulted in 11% higher grain yield (12 547 kg ha-1) than 90 cm rows (11 288 kg ha-1). Butternut yield increased significantly (p < 0.01) with increase in plant population and N rate. The optimum butternut population and N rate were estimated at 25 000 plants ha-1 and 120 kg N ha-1, respectively. Pre-plant weed control in butternut resulted in a six-fold decrease in weed biomass. Increasing butternut planting density from 10 000 to 30 000 plants ha-1 decreased weed biomass by 47%. No marketable fruits were obtained when planting was done without prior weed control. This result suggest that application of non-selective herbicide before planting is one technique that can be used by farmers as part of integrated weed management to give the crop a competitive advantage in the early growth stages before the plants start to produce vines. Yield increased significantly (p < 0.01) with increase in plant density and N rate. The optimum plant density and N rate were estimated at 25 000 plants ha-1 and 120 kg N ha-1, respectively. These findings emphasise the need to take into consideration production practices by farmers as a basis to build up skills in the management of crop enterprises and the farm as a viable business in any efforts to improve on the performance of SIS in South Africa. Future research should concentrate on labour-saving sustainable production technologies for SIS in South Africa.
- Full Text:
- Date Issued: 2010
- Authors: Fanadzo, Morris
- Date: 2010
- Subjects: Farms, Small -- South Africa -- Eastern Cape Crop yields -- South Africa -- Eastern Cape Agricultural productivity -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/11009 , vital:36450
- Description: Smallholder irrigation schemes (SIS) in South Africa have performed poorly and failed to achieve the development objectives of sustaining rural livelihoods by increasing crop production and income. Limited knowledge of crop production among farmers is one constraint to improved crop productivity and, yet little research has been carried out to relate agronomic practices to productivity. This study was aimed at identifying strategies to improve the productivity of maize-based smallholder irrigated cropping systems in South Africa, using Zanyokwe irrigation scheme (ZIS) in the Eastern Cape Province as a case study. The research design was emergent, developed progressively as influenced by the progressive analyses that were made. The research work involved two main phases: exploratory studies and refinement on-farm experiments for testing technologies as informed by the exploratory studies. Exploratory studies were aimed at describing farmer crop production practices in major crop enterprises and identifying and prioritizing major agronomic yield constraints. Findings indicated that the overall performance of the cropping systems was poor as evidenced by low overall yields of < 25% of the potential in maize and butternut. Poor management of weeds, soil fertility, plant populations and water, late planting and poor cultivar choice were the most important yield constraints. The average cropping intensity achieved by farmers was only 48%. New hybrids yielded 50 - 65% higher grain than the cultivars commonly grown by farmers. However, farmers favoured their traditional cultivars, as they preferred green maize production as opposed to dry grains. As a result of these findings, focused research was designed on options for iii improving green maize production, soil fertility, crop stand and weed management in crop production. On-farm trials were, therefore, designed to (1) compare the response to nitrogen (N) rate of direct seeded and transplanted maize; (2) evaluate comparative performance of direct seeded and transplanted green maize under farmer management, and to work out the economics of transplanting; (3) investigate the effects of row spacing and atrazine dosage on weed dynamics maize yield; (4) determine the effects of inter-row spacing and plant population on weed dynamics and maize yield; and (5) investigate the effects of pre-plant weed control, N rate and plant population on weed dynamics and butternut yield. Maize transplanting improved crop stand, had earlier maturity and yielded higher at lower N rates compared to direct seeding for both green and grain maize. Total costs that varied were higher by R3 565 ha-1 for transplanted maize compared to direct-seeded maize, but the net benefits from transplanting were also much higher by R8 773 ha-1. Farmers favoured transplanting, citing bigger cobs, early maturity and the absence of seedling damage by birds, when maize was transplanted. However, a follow-up survey conducted during the subsequent year indicated that five of the six farmers abandoned transplanting, citing shortage of labour and high cost of commercially produced seedlings. It was concluded that transplanting is unlikely to be adopted unless the labour intensiveness of manual transplanting can be solved. Increasing atrazine dosage from a third of the label recommended dosage (LRD) and reducing row spacing from 90 to 45 cm resulted in an increase in weed mortality. A 100% kill of annual broadleaf weeds was possible while iv perennial grasses and the annual broadleaf weed, Oxalis latifolia, could not be controlled even at the LRD. However, improved weed mortality with increase in atrazine dosage and/or narrow rows did not translate into improved maize productivity. In butternut, pre-plant weed control resulted in a six-fold decrease in weed biomass and a 100% increase in fruit yield. These findings demonstrated the possibility of incorporation of reduced herbicide dosages and narrow rows to achieve adequate weed control and optimise on maize yields in smallholder farming systems. The results also suggested that pre-plant weed control is one of the pre-requisite to successful butternut production in SIS in South Africa. Increasing maize population from 40 000 to 60 000 plants ha-1 resulted in a 30% grain yield increase, but decreased length of cobs. Row spacing had no effect on maize yield at 40 000 plants ha-1, but at 60 000 plants ha-1 45 cm rows resulted in 11% higher grain yield (12 547 kg ha-1) than 90 cm rows (11 288 kg ha-1). Butternut yield increased significantly (p < 0.01) with increase in plant population and N rate. The optimum butternut population and N rate were estimated at 25 000 plants ha-1 and 120 kg N ha-1, respectively. Pre-plant weed control in butternut resulted in a six-fold decrease in weed biomass. Increasing butternut planting density from 10 000 to 30 000 plants ha-1 decreased weed biomass by 47%. No marketable fruits were obtained when planting was done without prior weed control. This result suggest that application of non-selective herbicide before planting is one technique that can be used by farmers as part of integrated weed management to give the crop a competitive advantage in the early growth stages before the plants start to produce vines. Yield increased significantly (p < 0.01) with increase in plant density and N rate. The optimum plant density and N rate were estimated at 25 000 plants ha-1 and 120 kg N ha-1, respectively. These findings emphasise the need to take into consideration production practices by farmers as a basis to build up skills in the management of crop enterprises and the farm as a viable business in any efforts to improve on the performance of SIS in South Africa. Future research should concentrate on labour-saving sustainable production technologies for SIS in South Africa.
- Full Text:
- Date Issued: 2010
Improving sanitization and fertiliser value of dairy manure and waste paper mixtures enriched with rock phosphate through combined thermophilic composting and vermicomposting
- Authors: Mupondi, Lushian Tapiwa
- Date: 2010
- Subjects: Compost , Vermicomposting , Manures , Biodegradation , Waste paper -- South Africa -- Eastern Cape , Thermophilic bacteria , Fertilizers
- Language: English
- Type: Thesis , Doctoral , PhD (Soil Science)
- Identifier: vital:11961 , http://hdl.handle.net/10353/411 , Compost , Vermicomposting , Manures , Biodegradation , Waste paper -- South Africa -- Eastern Cape , Thermophilic bacteria , Fertilizers
- Description: Thermophilic composting (TC) and vermicomposting (V) are the two most common methods used for biological stabilization of solid organic wastes. Both have their advantages and disadvantages but the proposed method of combining composting and vermicomposting (CV) borrows pertinent attributes from each of the two methods and combines them to enhance overall process and product qualities. Dairy manure and waste paper are two wastes produced in large quantities at the University of Fort Hare. The study was carried out to address the following specific objectives, to determine (i) the effectiveness of combined thermophilic composting and vermicomposting on the biodegradation and sanitization of mixtures of dairy manure and paper waste, (ii) an optimum precomposting period for dairy manure paper waste mixtures that results in vermicomposts of good nutritional quality and whose use will not jeopardize human health, (iii) the effectiveness of phosphate rock (PR) in increasing available P and degradation and nutrient content of dairy manure-paper vermicomposts, (iv) the physicochemical properties of vermicompost substituted pine bark compost and performance of resultant growing medium on plant growth and nutrient uptake. Results of this study revealed that wastes with a C: N ratio of 30 were more suitable for both V and CV as their composts were more stabilized and with higher nutrient contents than composts made from wastes with a C: N ratio of 45. Both V and CV were effective methods for the biodegradation of dairy manure and paper waste mixtures with C: N ratio of 30 but the latter was more effective in the biodegradation of waste mixtures with a C: N ratio of 45. The combinination of composting and vermicomposting eliminated the indicator pathogen E. coli 0157 from the final composts whereas V only managed to reduce the pathogen population. iv A follow up study was done to determine the effects of precomposting on pathogen numbers so as to come up with a suitable precomposting period to use when combine composting dairy manure-waste paper mixtures. Results of this study showed that over 95% of fecal coliforms, E. coli and of E. coli 0157 were eliminated from the wastes within one week of precomposting and total elimination of these and protozoan (oo)cysts achieved after 3 weeks of precomposting. The vermicomposts pathogen content was related to the waste’s precomposting period. Final vermicomposts pathogen content was reduced and varied according to precomposting period. Vermicomposts from wastes precomposted for over two weeks were less stabilized, less humified and had less nutrient contents compared to vermicomposts from wastes that were precomposted for one week or less. The findings suggest that a precomposting period of one week is ideal for the effective vermicomposting of dairy manure-waste paper mixtures. Results of the P enrichment study indicated an increase in the inorganic phosphate and a reduction in the organic phosphate fractions of dairy manure-waste paper vermicompost that were enriched with PR. This implied an increase in mineralization of organic matter and or solubilization of PR with vermicomposting time. Applying PR to dairy manure-waste paper mixtures also enhanced degradation and had increased N and P contents of dairy manure-waste paper vermicomposts. Earthworms accumulated heavy metals in their bodies and reduced heavy metal contents of vermicomposts. A study to determine the physicochemical properties of vermicompost substituted pine bark compost and performance of resultant growing medium on plant growth and nutrient uptake was done. Results obtained revealed that increasing proportions of dairy manure vermicomposts in pine bark compost improved tomato plant height, stem girth, shoot and root dry weights. v Tomatoes grew best in the 40 to 60% CV substituted pine bark and application of Horticote (7:2:1 (22)) fertilizer significantly increased plant growth in all media. Progressive substitution pine bark with dairy manure vermicomposts resulted in a decrease in the percentage total porosity, percentage air space whilst bulk density, water holding capacity, particle density, pH, electrical conductivity and N and P levels increased. Precomposting wastes not only reduced and or eliminated pathogens but also improved the stabilisation and nutrient content of dairy manure waste paper mixtures. The application of PR to dairy manure waste paper mixtures improved the chemical and physical properties of vermicomposts. Earthworms bio-accumulated the heavy metals Cd, Cr, Cu, Pb and Zn whilst the contents of these in the vermicomposts declined. It is, therefore, recommended that dairy manure waste paper mixtures be precomposted for one week for sanitization followed by PR application and vermicomposting for stabilization and improved nutrients contents of resultant vermicomposts. Substitution of pine bark compost with 40 to 60 % PR-enriched vermicompost produced a growing medium with superior physical and chemical properties which supported good seedling growth. However, for optimum seedling growth, supplementation with mineral fertilizer was found to be necessary.
- Full Text:
- Date Issued: 2010
- Authors: Mupondi, Lushian Tapiwa
- Date: 2010
- Subjects: Compost , Vermicomposting , Manures , Biodegradation , Waste paper -- South Africa -- Eastern Cape , Thermophilic bacteria , Fertilizers
- Language: English
- Type: Thesis , Doctoral , PhD (Soil Science)
- Identifier: vital:11961 , http://hdl.handle.net/10353/411 , Compost , Vermicomposting , Manures , Biodegradation , Waste paper -- South Africa -- Eastern Cape , Thermophilic bacteria , Fertilizers
- Description: Thermophilic composting (TC) and vermicomposting (V) are the two most common methods used for biological stabilization of solid organic wastes. Both have their advantages and disadvantages but the proposed method of combining composting and vermicomposting (CV) borrows pertinent attributes from each of the two methods and combines them to enhance overall process and product qualities. Dairy manure and waste paper are two wastes produced in large quantities at the University of Fort Hare. The study was carried out to address the following specific objectives, to determine (i) the effectiveness of combined thermophilic composting and vermicomposting on the biodegradation and sanitization of mixtures of dairy manure and paper waste, (ii) an optimum precomposting period for dairy manure paper waste mixtures that results in vermicomposts of good nutritional quality and whose use will not jeopardize human health, (iii) the effectiveness of phosphate rock (PR) in increasing available P and degradation and nutrient content of dairy manure-paper vermicomposts, (iv) the physicochemical properties of vermicompost substituted pine bark compost and performance of resultant growing medium on plant growth and nutrient uptake. Results of this study revealed that wastes with a C: N ratio of 30 were more suitable for both V and CV as their composts were more stabilized and with higher nutrient contents than composts made from wastes with a C: N ratio of 45. Both V and CV were effective methods for the biodegradation of dairy manure and paper waste mixtures with C: N ratio of 30 but the latter was more effective in the biodegradation of waste mixtures with a C: N ratio of 45. The combinination of composting and vermicomposting eliminated the indicator pathogen E. coli 0157 from the final composts whereas V only managed to reduce the pathogen population. iv A follow up study was done to determine the effects of precomposting on pathogen numbers so as to come up with a suitable precomposting period to use when combine composting dairy manure-waste paper mixtures. Results of this study showed that over 95% of fecal coliforms, E. coli and of E. coli 0157 were eliminated from the wastes within one week of precomposting and total elimination of these and protozoan (oo)cysts achieved after 3 weeks of precomposting. The vermicomposts pathogen content was related to the waste’s precomposting period. Final vermicomposts pathogen content was reduced and varied according to precomposting period. Vermicomposts from wastes precomposted for over two weeks were less stabilized, less humified and had less nutrient contents compared to vermicomposts from wastes that were precomposted for one week or less. The findings suggest that a precomposting period of one week is ideal for the effective vermicomposting of dairy manure-waste paper mixtures. Results of the P enrichment study indicated an increase in the inorganic phosphate and a reduction in the organic phosphate fractions of dairy manure-waste paper vermicompost that were enriched with PR. This implied an increase in mineralization of organic matter and or solubilization of PR with vermicomposting time. Applying PR to dairy manure-waste paper mixtures also enhanced degradation and had increased N and P contents of dairy manure-waste paper vermicomposts. Earthworms accumulated heavy metals in their bodies and reduced heavy metal contents of vermicomposts. A study to determine the physicochemical properties of vermicompost substituted pine bark compost and performance of resultant growing medium on plant growth and nutrient uptake was done. Results obtained revealed that increasing proportions of dairy manure vermicomposts in pine bark compost improved tomato plant height, stem girth, shoot and root dry weights. v Tomatoes grew best in the 40 to 60% CV substituted pine bark and application of Horticote (7:2:1 (22)) fertilizer significantly increased plant growth in all media. Progressive substitution pine bark with dairy manure vermicomposts resulted in a decrease in the percentage total porosity, percentage air space whilst bulk density, water holding capacity, particle density, pH, electrical conductivity and N and P levels increased. Precomposting wastes not only reduced and or eliminated pathogens but also improved the stabilisation and nutrient content of dairy manure waste paper mixtures. The application of PR to dairy manure waste paper mixtures improved the chemical and physical properties of vermicomposts. Earthworms bio-accumulated the heavy metals Cd, Cr, Cu, Pb and Zn whilst the contents of these in the vermicomposts declined. It is, therefore, recommended that dairy manure waste paper mixtures be precomposted for one week for sanitization followed by PR application and vermicomposting for stabilization and improved nutrients contents of resultant vermicomposts. Substitution of pine bark compost with 40 to 60 % PR-enriched vermicompost produced a growing medium with superior physical and chemical properties which supported good seedling growth. However, for optimum seedling growth, supplementation with mineral fertilizer was found to be necessary.
- Full Text:
- Date Issued: 2010
Indigenous technology and culture in the technology curriculum : starting the conversation : a case study
- Authors: Vandeleur, Sonja
- Date: 2010
- Subjects: Curriculum evaluation -- South Africa Curriculum planning -- South Africa Education -- Curricula -- South Africa Technology -- Study and teaching -- South Africa Indigenous peoples -- Education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1573 , http://hdl.handle.net/10962/d1003455
- Description: Since the collapse of apartheid and the first democratic elections of 1994, education in South Africa has undergone fundamental transformation and part of this transformation was the reconstruction of the school curriculum. The new curriculum, known as Curriculum 2005 and developed in 1997, introduced Technology as a new learning area. This study is based on the inclusion of ‘indigenous technology and culture’, a new aspect introduced in a revision of Curriculum 2005. The broad goal of the study was to examine and explore pedagogic practice in relation to the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology. The study was informed by an examination of literature pertaining to philosophy of technology, indigenous knowledge systems and technology education. The review of the literature highlighted the contested nature of ‘indigenous knowledge systems’. Philosophies on the nature of technological knowledge were reviewed in order to explore the meaning of ‘technology’, and a comparative review of curriculum reform in regard to technology education in various parts of the world was conducted. This study presented an attempt to determine the rationale for the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology in South Africa and to explore and examine what teachers’ existing practices were in this regard. It also examined a process of participatory co-engagement with a focus group of teachers. This process was an attempt to implement ‘indigenous technology and culture’ of the curriculum in a more meaningful way. A case study approach using an in-depth, interpretive design was used. A questionnaire, document analysis, interviews and focus group discussions were used to conduct the investigation. What emerged from the data analysis was that there was unanimous support for the inclusion of ‘indigenous technology and culture’ in the technology curriculum, but implementation had been problematic. This was partly due to difficulties with the interpretation of this aspect in the curriculum as well as a lack of meaningful teaching and learning for various reasons. The study revealed that teachers face multiple dilemmas in implementing ‘indigenous technology and culture’ as an assessment standard. These dilemmas are pedagogical, political, conceptual, professional and cultural in nature. The intentions of the study were to build a comprehensive understanding of ‘indigenous technology and culture’ and to determine how a focus group of teachers were dealing with this new inclusion. The interpretive study concluded with implications and recommendations for further studies.
- Full Text:
- Date Issued: 2010
- Authors: Vandeleur, Sonja
- Date: 2010
- Subjects: Curriculum evaluation -- South Africa Curriculum planning -- South Africa Education -- Curricula -- South Africa Technology -- Study and teaching -- South Africa Indigenous peoples -- Education -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1573 , http://hdl.handle.net/10962/d1003455
- Description: Since the collapse of apartheid and the first democratic elections of 1994, education in South Africa has undergone fundamental transformation and part of this transformation was the reconstruction of the school curriculum. The new curriculum, known as Curriculum 2005 and developed in 1997, introduced Technology as a new learning area. This study is based on the inclusion of ‘indigenous technology and culture’, a new aspect introduced in a revision of Curriculum 2005. The broad goal of the study was to examine and explore pedagogic practice in relation to the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology. The study was informed by an examination of literature pertaining to philosophy of technology, indigenous knowledge systems and technology education. The review of the literature highlighted the contested nature of ‘indigenous knowledge systems’. Philosophies on the nature of technological knowledge were reviewed in order to explore the meaning of ‘technology’, and a comparative review of curriculum reform in regard to technology education in various parts of the world was conducted. This study presented an attempt to determine the rationale for the inclusion of ‘indigenous technology and culture’ in the revised National Curriculum Statement for Technology in South Africa and to explore and examine what teachers’ existing practices were in this regard. It also examined a process of participatory co-engagement with a focus group of teachers. This process was an attempt to implement ‘indigenous technology and culture’ of the curriculum in a more meaningful way. A case study approach using an in-depth, interpretive design was used. A questionnaire, document analysis, interviews and focus group discussions were used to conduct the investigation. What emerged from the data analysis was that there was unanimous support for the inclusion of ‘indigenous technology and culture’ in the technology curriculum, but implementation had been problematic. This was partly due to difficulties with the interpretation of this aspect in the curriculum as well as a lack of meaningful teaching and learning for various reasons. The study revealed that teachers face multiple dilemmas in implementing ‘indigenous technology and culture’ as an assessment standard. These dilemmas are pedagogical, political, conceptual, professional and cultural in nature. The intentions of the study were to build a comprehensive understanding of ‘indigenous technology and culture’ and to determine how a focus group of teachers were dealing with this new inclusion. The interpretive study concluded with implications and recommendations for further studies.
- Full Text:
- Date Issued: 2010
Induced plant responses of different Lantana camara L. (Verbenaceae) varieties to herbivory by Falconia intermedia (distant) (Hemiptera: Miridae)
- Heshula, Unathi-Nkosi Lelethu Peter
- Authors: Heshula, Unathi-Nkosi Lelethu Peter
- Date: 2010
- Subjects: Lantana camara -- South Africa -- Eastern Cape , Lantana camara -- Biological control -- South Africa -- Eastern Cape , Biological pest control agents -- South Africa -- Eastern Cape , Weeds -- Biological control -- South Africa -- Eastern Cape , Invasive plants -- Biological control -- South Africa -- Eastern Cape , Hemiptera -- South Africa -- Eastern Cape , Miridae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5911 , http://hdl.handle.net/10962/d1015368
- Description: A highly variable invasive shrub, Lantana camara L. (Verbenaceae), has been notoriously difficult to control thus far despite a well established biological control programme in South Africa. A promising leaf-feeding biological control agent, Falconia intermedia (Distant) (Hemiptera: Miridae), released to control this invasive plant eventually crashed at three out of five sites in the Eastern Cape Province. In the Mpumalanga Province, after initially colonising and building up high numbers on the L. camara stands the agent populations crashed. Several reasons for these population crashes have been suggested, but induced plant defences have not been investigated. Although plants face the challenge of herbivory by various organisms while remaining immobile, some plants may possess the ability to induce physical and/or chemical defensive responses following feeding and thus prevent further plant tissue damage and loss. Laboratory trials were conducted to determine the existence, nature and effect of physical and chemical feeding-induced responses of L. camara on the performance of the leaf-feeding biological control agent, F. intermedia. Lantana camara plants used in the study were obtained from five localities in the Eastern Cape Province, South Africa, while the insect culture was established from field populations. Plants from all varieties on which F. intermedia was released significantly increased the toughness of their leaves compared to control treatment plants. In addition, plants from three localities: Lyndhurst Farm, East London and Port Alfred, significantly increased trichome density after prolonged feeding by F. intermedia. On the three varieties showing increases in these two factors (i.e. leaf toughness and trichome density), oviposition, survival and feeding damage by the mirid agent was significantly lower on previously damaged plants. A significant negative correlation between trichome density and population numbers was found (R²= 0.52, p < 0.0003), suggesting that an increase in trichome density strongly contributes to a reduction in F. intermedia's growth. The growth and reproduction of the resistant plants was not significantly impacted by F. intermedia feeding. The defensive responses were found to be plant systemic and rapidly induced as they were elicited and expressed throughout the plant in both damaged and undamaged leaves within five weeks after insect release. Leaf toughness and trichome density were not significantly increased after feeding on plants from Whitney Farm and Heather Glen Farm. On the contrary, mirid individuals performed significantly better on plants from Whitney Farm and Heather Glen Farm than on plants of other varieties, indicating their susceptibility and suitability to the agent and the lack of induced resistance against the agent. Plants from all localities besides East London showed some level of tolerance and overcompensated for feeding damage by increasing plant growth and reproductive factors on plants fed upon. This was however only significant in two variables of the more susceptible localities, Whitney Farm and Heather Glen Farm. This increase in plant fitness did however indicate an induced defence response by these plants to feeding, a response designed to lessen the effects of agent feeding. Headspace volatile analysis was used to investigate any volatile chemical responses by L. camara due to F. intermedia feeding at two of the five localities chosen: East London and Whitney Farm. There was no significant difference in headspace volatiles emitted by leaves of plants from the East London insect infested and control treatment plants. On the Whitney Farm damaged plants however there was a 2.5 fold increase in the emission intensity of one of the three main compounds, later identified as Beta-caryophyllene. Three major chemical constituents which were found to be common to leaf volatiles of the two varieties were identified through gas chromatography-mass spectrometry (GC-MS) from the damaged and undamaged leaves of these two varieties. The methods used in collecting leaf volatiles were shown to be significant in the strength of chromatogram peaks. Using general authentication methods and purified standards, one of these was identified as the sesquiterpene, Beta-caryophyllene (C₁₅H₂₄). This compound is one of the major constituents found in isolations of L. camara varieties worldwide. This is the first such work done on a variety of L. camara in South Africa, and hopefully the beginning of more in-depth studies of the volatile organic chemicals from the numerous naturalised varieties of L. camara. It is suggested that the sum of these responses may play a role bigger than is currently understood in this plant-insect relationship. It is also argued that feeding induced plant defences may play an important role in attempts to control alien plants using insect agents.
- Full Text:
- Date Issued: 2010
- Authors: Heshula, Unathi-Nkosi Lelethu Peter
- Date: 2010
- Subjects: Lantana camara -- South Africa -- Eastern Cape , Lantana camara -- Biological control -- South Africa -- Eastern Cape , Biological pest control agents -- South Africa -- Eastern Cape , Weeds -- Biological control -- South Africa -- Eastern Cape , Invasive plants -- Biological control -- South Africa -- Eastern Cape , Hemiptera -- South Africa -- Eastern Cape , Miridae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5911 , http://hdl.handle.net/10962/d1015368
- Description: A highly variable invasive shrub, Lantana camara L. (Verbenaceae), has been notoriously difficult to control thus far despite a well established biological control programme in South Africa. A promising leaf-feeding biological control agent, Falconia intermedia (Distant) (Hemiptera: Miridae), released to control this invasive plant eventually crashed at three out of five sites in the Eastern Cape Province. In the Mpumalanga Province, after initially colonising and building up high numbers on the L. camara stands the agent populations crashed. Several reasons for these population crashes have been suggested, but induced plant defences have not been investigated. Although plants face the challenge of herbivory by various organisms while remaining immobile, some plants may possess the ability to induce physical and/or chemical defensive responses following feeding and thus prevent further plant tissue damage and loss. Laboratory trials were conducted to determine the existence, nature and effect of physical and chemical feeding-induced responses of L. camara on the performance of the leaf-feeding biological control agent, F. intermedia. Lantana camara plants used in the study were obtained from five localities in the Eastern Cape Province, South Africa, while the insect culture was established from field populations. Plants from all varieties on which F. intermedia was released significantly increased the toughness of their leaves compared to control treatment plants. In addition, plants from three localities: Lyndhurst Farm, East London and Port Alfred, significantly increased trichome density after prolonged feeding by F. intermedia. On the three varieties showing increases in these two factors (i.e. leaf toughness and trichome density), oviposition, survival and feeding damage by the mirid agent was significantly lower on previously damaged plants. A significant negative correlation between trichome density and population numbers was found (R²= 0.52, p < 0.0003), suggesting that an increase in trichome density strongly contributes to a reduction in F. intermedia's growth. The growth and reproduction of the resistant plants was not significantly impacted by F. intermedia feeding. The defensive responses were found to be plant systemic and rapidly induced as they were elicited and expressed throughout the plant in both damaged and undamaged leaves within five weeks after insect release. Leaf toughness and trichome density were not significantly increased after feeding on plants from Whitney Farm and Heather Glen Farm. On the contrary, mirid individuals performed significantly better on plants from Whitney Farm and Heather Glen Farm than on plants of other varieties, indicating their susceptibility and suitability to the agent and the lack of induced resistance against the agent. Plants from all localities besides East London showed some level of tolerance and overcompensated for feeding damage by increasing plant growth and reproductive factors on plants fed upon. This was however only significant in two variables of the more susceptible localities, Whitney Farm and Heather Glen Farm. This increase in plant fitness did however indicate an induced defence response by these plants to feeding, a response designed to lessen the effects of agent feeding. Headspace volatile analysis was used to investigate any volatile chemical responses by L. camara due to F. intermedia feeding at two of the five localities chosen: East London and Whitney Farm. There was no significant difference in headspace volatiles emitted by leaves of plants from the East London insect infested and control treatment plants. On the Whitney Farm damaged plants however there was a 2.5 fold increase in the emission intensity of one of the three main compounds, later identified as Beta-caryophyllene. Three major chemical constituents which were found to be common to leaf volatiles of the two varieties were identified through gas chromatography-mass spectrometry (GC-MS) from the damaged and undamaged leaves of these two varieties. The methods used in collecting leaf volatiles were shown to be significant in the strength of chromatogram peaks. Using general authentication methods and purified standards, one of these was identified as the sesquiterpene, Beta-caryophyllene (C₁₅H₂₄). This compound is one of the major constituents found in isolations of L. camara varieties worldwide. This is the first such work done on a variety of L. camara in South Africa, and hopefully the beginning of more in-depth studies of the volatile organic chemicals from the numerous naturalised varieties of L. camara. It is suggested that the sum of these responses may play a role bigger than is currently understood in this plant-insect relationship. It is also argued that feeding induced plant defences may play an important role in attempts to control alien plants using insect agents.
- Full Text:
- Date Issued: 2010
Influences on construction project delivery time
- Authors: Olatunji, Aiyetan Ayodeji
- Date: 2010
- Subjects: Construction industry -- South Africa -- Management , Project management -- South Africa , Construction industry -- Economic aspects -- South Africa , Construction industry -- Technological innovations -- South Africa , Construction industry -- Labor productivity -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9681 , http://hdl.handle.net/10948/1548 , Construction industry -- South Africa -- Management , Project management -- South Africa , Construction industry -- Economic aspects -- South Africa , Construction industry -- Technological innovations -- South Africa , Construction industry -- Labor productivity -- South Africa
- Description: Construction delays are a global phenomenon. Factors causing construction delays in construction projects differ from country to country, due to different prevailing conditions. The prevailing conditions that could exert an influence on project delivery time are: political, economic, and physical factors as well as level of technological development; management style, and construction techniques. The construction industry is a major player in the economy, generating both employment and wealth. However, many projects experience extensive delays and thereby exceed initial time and cost estimates. This study aims at determining the causes of delays in project delivery in South Africa; evolving interventions, and developing a model for the delivery of projects on time. Inferential and linear regression statistical tools were used in the analysis of data for the study. The sample population consists of architects, builders, quantity surveyors, structural engineers, and clients, and the metropolitan cities of five provinces constituted the geographical delimitation of the study. The provinces are: Eastern Cape; Free State; Gauteng; KwaZulu-Natal, and Western Cape. The metropolitan cities are: Bloemfontein; Cape Town; Durban; Johannesburg, and Port Elizabeth. Findings which negatively influence project delivery time in South Africa include the following: lack of adequate planning; management style; the lack of constructability reviews of designs; inadequate motivation of workers; economic policies; lack of prompt payment to contractors, and quality of management during design and construction. Recommendations include: (1) The introduction of the following courses in built environment tertiary education � quality management competences; operational planning; design management, and generic management; (2) pre-qualification of suppliers; (3) inclusion of the following in tender documentation � human resource schedule; plant and equipment schedule; quality assurance plan, and work schedule; (4) appointment of materials specialists on a large projects; (5) the model developed should be adopted for use in the South African construction industry for the delivery of projects on time, and (6) the linear regression equation: Y = 13.1159 + 1.1341x or 35.3 percent addition on time for the estimation of project delivery time.
- Full Text:
- Date Issued: 2010
- Authors: Olatunji, Aiyetan Ayodeji
- Date: 2010
- Subjects: Construction industry -- South Africa -- Management , Project management -- South Africa , Construction industry -- Economic aspects -- South Africa , Construction industry -- Technological innovations -- South Africa , Construction industry -- Labor productivity -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9681 , http://hdl.handle.net/10948/1548 , Construction industry -- South Africa -- Management , Project management -- South Africa , Construction industry -- Economic aspects -- South Africa , Construction industry -- Technological innovations -- South Africa , Construction industry -- Labor productivity -- South Africa
- Description: Construction delays are a global phenomenon. Factors causing construction delays in construction projects differ from country to country, due to different prevailing conditions. The prevailing conditions that could exert an influence on project delivery time are: political, economic, and physical factors as well as level of technological development; management style, and construction techniques. The construction industry is a major player in the economy, generating both employment and wealth. However, many projects experience extensive delays and thereby exceed initial time and cost estimates. This study aims at determining the causes of delays in project delivery in South Africa; evolving interventions, and developing a model for the delivery of projects on time. Inferential and linear regression statistical tools were used in the analysis of data for the study. The sample population consists of architects, builders, quantity surveyors, structural engineers, and clients, and the metropolitan cities of five provinces constituted the geographical delimitation of the study. The provinces are: Eastern Cape; Free State; Gauteng; KwaZulu-Natal, and Western Cape. The metropolitan cities are: Bloemfontein; Cape Town; Durban; Johannesburg, and Port Elizabeth. Findings which negatively influence project delivery time in South Africa include the following: lack of adequate planning; management style; the lack of constructability reviews of designs; inadequate motivation of workers; economic policies; lack of prompt payment to contractors, and quality of management during design and construction. Recommendations include: (1) The introduction of the following courses in built environment tertiary education � quality management competences; operational planning; design management, and generic management; (2) pre-qualification of suppliers; (3) inclusion of the following in tender documentation � human resource schedule; plant and equipment schedule; quality assurance plan, and work schedule; (4) appointment of materials specialists on a large projects; (5) the model developed should be adopted for use in the South African construction industry for the delivery of projects on time, and (6) the linear regression equation: Y = 13.1159 + 1.1341x or 35.3 percent addition on time for the estimation of project delivery time.
- Full Text:
- Date Issued: 2010
Integrated teaching strategies model for improved scientific literacy in second-language learners
- Villanueva, Mary Grace Flores
- Authors: Villanueva, Mary Grace Flores
- Date: 2010
- Subjects: Science -- Study and teaching (Elementary) -- South Africa , Science -- South Africa -- Methodology , Science -- Curricula
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9514 , http://hdl.handle.net/10948/1107 , Science -- Study and teaching (Elementary) -- South Africa , Science -- South Africa -- Methodology , Science -- Curricula
- Description: The importance of a scientifically literate society is currently acknowledged both internationally and South Africa. The notion of scientific literacy in South Africa has emerged largely due to the government’s recognition of the role that science and technology plays in economic growth, employment creation, social redress and social development. However, in light of South Africa’s learner performance on international and national assessments such as TIMMS (2003) and PIRLS (2006), as well as the problems of teaching and learning in a second language, there appears to be a primary and pressing need to develop learners’ fundamental sense of scientific literacy (Norris & Phillips, 2003). Expanding learners’ ability to read, write and communicate in science may provide the necessary framework for engaging learners in the critical principles and foundations of the scientific endeavour (Hand, Prain, & Yore, 2001). As such, this study focuses on equipping and training grade six and seven science teachers to develop scientifically literate learners via professional development workshops with a strategy that supports reading, writing, talking and conducting (‘doing’) science through scientific investigations. The typology of triangulation and the mixed method research approach was supported by a fully mixed, concurrent, and equal status design (Leech & Onwuegbuzi, 2007). Quantitative data were collected from the baseline and post-intervention testing of learners’ problem solving skills, as well as their literacy skills in English and isiXhosa. Qualitative measures were generated through classroom observations, teacher interviews and learners’ science notebooks. The study was conducted in two different milieus in the Eastern Cape, South Africa. The first setting, in the rural area of Tyumie Valley near the Hogsback Mountains, was comprised of a sample of grade six and seven (multi-grade classrooms) teachers (n=7) and learners (n=168) from five experimental schools and two comparison schools. The second setting, in the urban townships area east of Port Elizabeth, was comprised of a sample of grade six teachers (n=8) and learners (n=675) from six experimental schools and two comparison schools. Mean differences between the experimental and the comparison groups were computed for the Raven’s Standard Progressive Matrices (RSPM) and the literacy tests, and the data generated were treated with an Analysis of Covariance (ANCOVA). The data suggest that the scientific literacy strategy improved the experimental learners’ problem solving skills. Both experimental groups demonstrated greater gains than that of the comparison schools. However, statistically significant improvements were only detected in Port Elizabeth. Improvements in learners’ literacy skills in isiXhosa and English varied according to each milieu. While the teachers initially identified challenges to learners’ reading and writing abilities, the analysis of learners’ science notebooks suggested that they used writings to support their investigations. Some teachers cited difficulties with certain aspects of the model, such as problems with developing an investigable question and argumentation, yet overall, teachers found the strategy useful for developing learners’ language skills, as well for strengthening their pedagogical practices in science. Teachers’ gradual improvements in the use of the model suggest that they were able to use the scientific literacy strategy to support the cognitive and linguistic development of second-language learners.
- Full Text:
- Date Issued: 2010
- Authors: Villanueva, Mary Grace Flores
- Date: 2010
- Subjects: Science -- Study and teaching (Elementary) -- South Africa , Science -- South Africa -- Methodology , Science -- Curricula
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9514 , http://hdl.handle.net/10948/1107 , Science -- Study and teaching (Elementary) -- South Africa , Science -- South Africa -- Methodology , Science -- Curricula
- Description: The importance of a scientifically literate society is currently acknowledged both internationally and South Africa. The notion of scientific literacy in South Africa has emerged largely due to the government’s recognition of the role that science and technology plays in economic growth, employment creation, social redress and social development. However, in light of South Africa’s learner performance on international and national assessments such as TIMMS (2003) and PIRLS (2006), as well as the problems of teaching and learning in a second language, there appears to be a primary and pressing need to develop learners’ fundamental sense of scientific literacy (Norris & Phillips, 2003). Expanding learners’ ability to read, write and communicate in science may provide the necessary framework for engaging learners in the critical principles and foundations of the scientific endeavour (Hand, Prain, & Yore, 2001). As such, this study focuses on equipping and training grade six and seven science teachers to develop scientifically literate learners via professional development workshops with a strategy that supports reading, writing, talking and conducting (‘doing’) science through scientific investigations. The typology of triangulation and the mixed method research approach was supported by a fully mixed, concurrent, and equal status design (Leech & Onwuegbuzi, 2007). Quantitative data were collected from the baseline and post-intervention testing of learners’ problem solving skills, as well as their literacy skills in English and isiXhosa. Qualitative measures were generated through classroom observations, teacher interviews and learners’ science notebooks. The study was conducted in two different milieus in the Eastern Cape, South Africa. The first setting, in the rural area of Tyumie Valley near the Hogsback Mountains, was comprised of a sample of grade six and seven (multi-grade classrooms) teachers (n=7) and learners (n=168) from five experimental schools and two comparison schools. The second setting, in the urban townships area east of Port Elizabeth, was comprised of a sample of grade six teachers (n=8) and learners (n=675) from six experimental schools and two comparison schools. Mean differences between the experimental and the comparison groups were computed for the Raven’s Standard Progressive Matrices (RSPM) and the literacy tests, and the data generated were treated with an Analysis of Covariance (ANCOVA). The data suggest that the scientific literacy strategy improved the experimental learners’ problem solving skills. Both experimental groups demonstrated greater gains than that of the comparison schools. However, statistically significant improvements were only detected in Port Elizabeth. Improvements in learners’ literacy skills in isiXhosa and English varied according to each milieu. While the teachers initially identified challenges to learners’ reading and writing abilities, the analysis of learners’ science notebooks suggested that they used writings to support their investigations. Some teachers cited difficulties with certain aspects of the model, such as problems with developing an investigable question and argumentation, yet overall, teachers found the strategy useful for developing learners’ language skills, as well for strengthening their pedagogical practices in science. Teachers’ gradual improvements in the use of the model suggest that they were able to use the scientific literacy strategy to support the cognitive and linguistic development of second-language learners.
- Full Text:
- Date Issued: 2010
Intertidal patterns and processes : tracking the effects of coastline topography and settlement choice across life stages of the mussels perna perna and mytilus galloprovincialis
- Von Der Meden, Charles Eric Otto
- Authors: Von Der Meden, Charles Eric Otto
- Date: 2010
- Subjects: Mexilhao mussel -- Behavior -- South Africa Mytilus galloprovincialis -- Behavior -- South Africa Perna -- Behavior -- South Africa Mussels -- Ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5721 , http://hdl.handle.net/10962/d1005407
- Description: Within landscapes, spatial heterogeneity is common and specific landscape features can influence propagule dispersal by wind or water, affecting population connectivity and dynamics. Coastline topographic features, such as bays and headlands, have a variety of biophysical effects on nearshore oceanography, larval transport, retention and supply, and the processes of larval settlement and recruitment. Although this has been demonstrated in several parts of the world, engendering a perception of a general ‘bay effect’, few studies have investigated this generality in a single experiment or region, by replicating at the level of ‘bay’. The Agulhas biogeographic region of the south coast of South Africa is a useful system within which to test for such generality. Using the intertidal mussels Mytilus galloprovincialis and Perna perna as model organisms, patterns of adult distribution were surveyed across four large ‘halfheart’ bays and intervening stretches of open coast, providing replication at the level of ‘bay’ and duplication of ecologically similar species. In support of a general, pervasive influence of bays on intertidal populations, mussel cover was found to be greater in bays than on the open coast for both species, although the effect was strongest for M. galloprovincialis. To explain this adult distribution, settlement, post-settlement mortality and recruitment were examined over 12mo at the same sites, with the prediction that rates of each would favour larger bay populations. Contrary to this, an interaction between month and bay-status was found, with greater settlement and recruitment on the open coast than in bays reflecting extreme settlement and recruitment events at 3 westerly open coast sites during summer. Re-analysis excluding these outliers, revealed the expected effect, of greater settlement and recruitment in bays. While this indicates the broad generality of the bay effect, it highlights exceptions and the need for replication in time and space when examining landscape effects. Measuring post-settlement mortality required testing small-scale settlement behaviour on established and newly deployed settler collectors. It was found that all settlers preferred collectors with biofilm, but that primary settlers avoided conspecific settlers, while secondary settlers were attracted to them. With discrepancies in settler attraction to new and established collectors accounted for, initial (over 2d) and longer-term (over 7d) post-settlement mortality rates were found to be substantial (ca 60 %) for both species. No topographic effect on p-s mortality was evident. Finally, recruit-settler, adult-recruit and interspecies correlations were examined at regional and local scales. Synergistic (or neutral) effects maintained the initial settlement pattern in recruit and adult populations regionally, but not at local scales; striking interspecies correlations suggested the influence of common regional transport processes. Ultimately, the results emphasize the importance of the direction of effects in different life stages and at different spatial scales, and the possibility that antagonistic effects may mask even strong patterns.
- Full Text:
- Date Issued: 2010
- Authors: Von Der Meden, Charles Eric Otto
- Date: 2010
- Subjects: Mexilhao mussel -- Behavior -- South Africa Mytilus galloprovincialis -- Behavior -- South Africa Perna -- Behavior -- South Africa Mussels -- Ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5721 , http://hdl.handle.net/10962/d1005407
- Description: Within landscapes, spatial heterogeneity is common and specific landscape features can influence propagule dispersal by wind or water, affecting population connectivity and dynamics. Coastline topographic features, such as bays and headlands, have a variety of biophysical effects on nearshore oceanography, larval transport, retention and supply, and the processes of larval settlement and recruitment. Although this has been demonstrated in several parts of the world, engendering a perception of a general ‘bay effect’, few studies have investigated this generality in a single experiment or region, by replicating at the level of ‘bay’. The Agulhas biogeographic region of the south coast of South Africa is a useful system within which to test for such generality. Using the intertidal mussels Mytilus galloprovincialis and Perna perna as model organisms, patterns of adult distribution were surveyed across four large ‘halfheart’ bays and intervening stretches of open coast, providing replication at the level of ‘bay’ and duplication of ecologically similar species. In support of a general, pervasive influence of bays on intertidal populations, mussel cover was found to be greater in bays than on the open coast for both species, although the effect was strongest for M. galloprovincialis. To explain this adult distribution, settlement, post-settlement mortality and recruitment were examined over 12mo at the same sites, with the prediction that rates of each would favour larger bay populations. Contrary to this, an interaction between month and bay-status was found, with greater settlement and recruitment on the open coast than in bays reflecting extreme settlement and recruitment events at 3 westerly open coast sites during summer. Re-analysis excluding these outliers, revealed the expected effect, of greater settlement and recruitment in bays. While this indicates the broad generality of the bay effect, it highlights exceptions and the need for replication in time and space when examining landscape effects. Measuring post-settlement mortality required testing small-scale settlement behaviour on established and newly deployed settler collectors. It was found that all settlers preferred collectors with biofilm, but that primary settlers avoided conspecific settlers, while secondary settlers were attracted to them. With discrepancies in settler attraction to new and established collectors accounted for, initial (over 2d) and longer-term (over 7d) post-settlement mortality rates were found to be substantial (ca 60 %) for both species. No topographic effect on p-s mortality was evident. Finally, recruit-settler, adult-recruit and interspecies correlations were examined at regional and local scales. Synergistic (or neutral) effects maintained the initial settlement pattern in recruit and adult populations regionally, but not at local scales; striking interspecies correlations suggested the influence of common regional transport processes. Ultimately, the results emphasize the importance of the direction of effects in different life stages and at different spatial scales, and the possibility that antagonistic effects may mask even strong patterns.
- Full Text:
- Date Issued: 2010
Investigating science teachers' perceptions of the nature of science in the context of curriculum reform in South Africa
- Kurup, Rajasekhar Thanukkothu Sankar Pillai
- Authors: Kurup, Rajasekhar Thanukkothu Sankar Pillai
- Date: 2010
- Subjects: Science -- Methodology -- South Africa , Science -- Study and teaching -- Philosophy , Science -- Study and teaching -- South Africa -- Evaluation , Science -- Study and teaching -- Curricula -- South Africa , Science -- Study and teaching -- South Africa
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9509 , http://hdl.handle.net/10948/1175 , Science -- Methodology -- South Africa , Science -- Study and teaching -- Philosophy , Science -- Study and teaching -- South Africa -- Evaluation , Science -- Study and teaching -- Curricula -- South Africa , Science -- Study and teaching -- South Africa
- Description: An adequate understanding of the nature of science (NOS) has become increasingly important for science teachers in South Africa as comprehensive curricular reforms over the past decade include promoting informed understandings of the ontological and epistemological bases of scientific knowledge and the methods of science. The main objective of this study was to explore the NOS understandings held by a sample of science teachers in the Eastern Cape Province of South Africa. Data were generated via questionnaires (n=136), semi-structured interviews (n=31), and classroom observations (n=8). The teacher interviews, which were informed by the questionnaire data, enabled further interrogation of the teachers’ philosophical positions. Their classroom practices were examined within the framework of these philosophical positions and the requirements of the new curriculum. The effect of implicit and explicit instruction in NOS on these teachers’ beliefs and classroom activities was also considered. A mixed-method approach informed by positivist and interpretivist perspectives was used for the collection and analysis of the data. The data suggests that explicit instruction in NOS resulted in more informed conceptions of science and the scientific enterprise, and that these conceptions were reflected, to a degree, in their classroom behaviours. However, it was noted that the teachers in this study often held philosophically eclectic views of the nature of scientific knowledge and how scientists develop ideas. Similarly, the South African National Curriculum Statement portrays science in contrasting ways, i.e. often within a modern/realist framework, but in other instances within postmodern/relativistic understandings (particularly in terms of indigenous knowledge systems). As such, an approach which aims at providing a firm foundation for understanding NOS ideas within a modern/realist perspective before emphasising the postmodern/relativist aspects of the scientific enterprise is suggested for teacher training and curriculum development.
- Full Text:
- Date Issued: 2010
- Authors: Kurup, Rajasekhar Thanukkothu Sankar Pillai
- Date: 2010
- Subjects: Science -- Methodology -- South Africa , Science -- Study and teaching -- Philosophy , Science -- Study and teaching -- South Africa -- Evaluation , Science -- Study and teaching -- Curricula -- South Africa , Science -- Study and teaching -- South Africa
- Language: English
- Type: Thesis , Doctoral , DEd
- Identifier: vital:9509 , http://hdl.handle.net/10948/1175 , Science -- Methodology -- South Africa , Science -- Study and teaching -- Philosophy , Science -- Study and teaching -- South Africa -- Evaluation , Science -- Study and teaching -- Curricula -- South Africa , Science -- Study and teaching -- South Africa
- Description: An adequate understanding of the nature of science (NOS) has become increasingly important for science teachers in South Africa as comprehensive curricular reforms over the past decade include promoting informed understandings of the ontological and epistemological bases of scientific knowledge and the methods of science. The main objective of this study was to explore the NOS understandings held by a sample of science teachers in the Eastern Cape Province of South Africa. Data were generated via questionnaires (n=136), semi-structured interviews (n=31), and classroom observations (n=8). The teacher interviews, which were informed by the questionnaire data, enabled further interrogation of the teachers’ philosophical positions. Their classroom practices were examined within the framework of these philosophical positions and the requirements of the new curriculum. The effect of implicit and explicit instruction in NOS on these teachers’ beliefs and classroom activities was also considered. A mixed-method approach informed by positivist and interpretivist perspectives was used for the collection and analysis of the data. The data suggests that explicit instruction in NOS resulted in more informed conceptions of science and the scientific enterprise, and that these conceptions were reflected, to a degree, in their classroom behaviours. However, it was noted that the teachers in this study often held philosophically eclectic views of the nature of scientific knowledge and how scientists develop ideas. Similarly, the South African National Curriculum Statement portrays science in contrasting ways, i.e. often within a modern/realist framework, but in other instances within postmodern/relativistic understandings (particularly in terms of indigenous knowledge systems). As such, an approach which aims at providing a firm foundation for understanding NOS ideas within a modern/realist perspective before emphasising the postmodern/relativist aspects of the scientific enterprise is suggested for teacher training and curriculum development.
- Full Text:
- Date Issued: 2010
Investigations of the bioavailability/bioequivalence of topical corticosteroid formulations containing clobetasol propionate using the human skin blanching assay, tape stripping and microdialysis
- Authors: Au, Wai Ling
- Date: 2010
- Subjects: Adrenocortical hormones -- Bioavailability , Drugs -- Therapeutic equivalency , Adrenocortical hormones -- Effectiveness , Adrenocortical hormones -- Testing , Adrenocortical hormones -- Side effects , Transdermal medication
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3743 , http://hdl.handle.net/10962/d1003221 , Adrenocortical hormones -- Bioavailability , Drugs -- Therapeutic equivalency , Adrenocortical hormones -- Effectiveness , Adrenocortical hormones -- Testing , Adrenocortical hormones -- Side effects , Transdermal medication
- Description: Currently, clinical trials in patients are required by most regulatory authorities for the assessment of bioequivalence of topical products where the drug is not intended for systemic absorption. Hence there is a dire need for suitable methods for the assessment of bioavailability and bioequivalence of such products since clinical safety and efficacy studies are expensive, time-consuming and require very large numbers of patients. Except for topical corticosteroid products where the human skin blanching assay/vasoconstrictor assay has been approved by the US FDA for bioequivalence assessment of those products, no other method has been “officially” approved for use in those investigations. However, a few alternative methods such as tape stripping and microdialysis have been pursued and considered to have the potential for use in ioequivalence/bioavailability studies. The human skin blanching assay was used to assess the bioequivalence of commercially available topical products containing 0.05% clobetasol propionate. Both visual and chromameter data were obtained and a commercially available topical corticosteroid product, Dermovate® cream was used as both the “Test” and the “Reference” product. The results indicated that both visual and chromametric assessments were comparable to each other and that either could be used for the assessment of the bioequivalence of topical products containing clobetasol propionate. The screening procedure was optimized to identify potential “detectors” for inclusion in the bioequivalence studies. This resulted in fewer subjects being required in a bioequivalence pivotal study, still having the necessary power to confirm bioequivalence using the human skin blanching assay. Another objective of this research was to re-visit tape stripping and other possible alternative methods such as dermal microdialysis and to optimize these procedures for bioequivalence assessment of topical formulations where the drug is not intended for systemic absorption. In the past few decades, tape stripping has been used to investigate bioavailability/bioequivalence of various topical formulations. This technique involves the removal of the stratum corneum to assess drug penetration through the skin. A draft FDA guidance for tape stripping was initially published but was subsequently withdrawn due to high variability and poor reproducibility. This research project used an optimized tape stripping procedure to determine bioavailability and establish bioequivalence between three commercially available formulations containing 0.05 % m/m clobetasol propionate. Furthermore, tape stripping was validated by undertaking a study to assess the bioequivalence of a 0.05% topical cream formulation (Dermovate® cream) using the same cream as both the “Test” and “Reference” product, in which bioequivalence was confirmed. The findings highlight the potential of tape stripping as an alternative method for the assessment of bioequivalence of clobetasol propionate formulations and may possibly be extended for use in other topical products. Microdialysis is another useful technique that can assess the penetration of topically applied substances which diffuses through the stratum corneum and into the dermis. Microdialysis has previously been successfully used for in vivo bioavailability and bioequivalence assessments of topical formulations. However, the drugs which were under investigation were all hydrophilic in nature. A major problem with the use of microdialysis for the assessment of lipophilic substances is the binding/adherence of the substance to the membrane and other components of the microdialysis system. As a result, this necessitates the development of a microdialysis system which can be used to assess lipophilic drugs. Intralipid® 20% was investigated and successfully utilized as a perfusate to recover a lipophilic topical corticosteroid, clobetasol propionate, in microdialysis studies. Hence, the bioavailability of clobetasol propionate from an extemporaneous preparation was determined in healthy human volunteers using microdialysis. These findings indicate that in vivo microdialysis can be used to assess lipophilic drug penetration through the skin. A novel approach to investigate drug release from topical formulations containing 0.05% clobetasol propionate using in vitro microdialysis was also undertaken. The in vitro findings were found to be in agreement with the results obtained using tape stripping to assess bioequivalence of the same commercially available products, namely Dermovate® cream, Dovate® Cream and Dermovate® ointment. These results indicate the potential to correlate in vitro with in vivo data for bioequivalence assessment of such topical dosage forms.
- Full Text:
- Date Issued: 2010
- Authors: Au, Wai Ling
- Date: 2010
- Subjects: Adrenocortical hormones -- Bioavailability , Drugs -- Therapeutic equivalency , Adrenocortical hormones -- Effectiveness , Adrenocortical hormones -- Testing , Adrenocortical hormones -- Side effects , Transdermal medication
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3743 , http://hdl.handle.net/10962/d1003221 , Adrenocortical hormones -- Bioavailability , Drugs -- Therapeutic equivalency , Adrenocortical hormones -- Effectiveness , Adrenocortical hormones -- Testing , Adrenocortical hormones -- Side effects , Transdermal medication
- Description: Currently, clinical trials in patients are required by most regulatory authorities for the assessment of bioequivalence of topical products where the drug is not intended for systemic absorption. Hence there is a dire need for suitable methods for the assessment of bioavailability and bioequivalence of such products since clinical safety and efficacy studies are expensive, time-consuming and require very large numbers of patients. Except for topical corticosteroid products where the human skin blanching assay/vasoconstrictor assay has been approved by the US FDA for bioequivalence assessment of those products, no other method has been “officially” approved for use in those investigations. However, a few alternative methods such as tape stripping and microdialysis have been pursued and considered to have the potential for use in ioequivalence/bioavailability studies. The human skin blanching assay was used to assess the bioequivalence of commercially available topical products containing 0.05% clobetasol propionate. Both visual and chromameter data were obtained and a commercially available topical corticosteroid product, Dermovate® cream was used as both the “Test” and the “Reference” product. The results indicated that both visual and chromametric assessments were comparable to each other and that either could be used for the assessment of the bioequivalence of topical products containing clobetasol propionate. The screening procedure was optimized to identify potential “detectors” for inclusion in the bioequivalence studies. This resulted in fewer subjects being required in a bioequivalence pivotal study, still having the necessary power to confirm bioequivalence using the human skin blanching assay. Another objective of this research was to re-visit tape stripping and other possible alternative methods such as dermal microdialysis and to optimize these procedures for bioequivalence assessment of topical formulations where the drug is not intended for systemic absorption. In the past few decades, tape stripping has been used to investigate bioavailability/bioequivalence of various topical formulations. This technique involves the removal of the stratum corneum to assess drug penetration through the skin. A draft FDA guidance for tape stripping was initially published but was subsequently withdrawn due to high variability and poor reproducibility. This research project used an optimized tape stripping procedure to determine bioavailability and establish bioequivalence between three commercially available formulations containing 0.05 % m/m clobetasol propionate. Furthermore, tape stripping was validated by undertaking a study to assess the bioequivalence of a 0.05% topical cream formulation (Dermovate® cream) using the same cream as both the “Test” and “Reference” product, in which bioequivalence was confirmed. The findings highlight the potential of tape stripping as an alternative method for the assessment of bioequivalence of clobetasol propionate formulations and may possibly be extended for use in other topical products. Microdialysis is another useful technique that can assess the penetration of topically applied substances which diffuses through the stratum corneum and into the dermis. Microdialysis has previously been successfully used for in vivo bioavailability and bioequivalence assessments of topical formulations. However, the drugs which were under investigation were all hydrophilic in nature. A major problem with the use of microdialysis for the assessment of lipophilic substances is the binding/adherence of the substance to the membrane and other components of the microdialysis system. As a result, this necessitates the development of a microdialysis system which can be used to assess lipophilic drugs. Intralipid® 20% was investigated and successfully utilized as a perfusate to recover a lipophilic topical corticosteroid, clobetasol propionate, in microdialysis studies. Hence, the bioavailability of clobetasol propionate from an extemporaneous preparation was determined in healthy human volunteers using microdialysis. These findings indicate that in vivo microdialysis can be used to assess lipophilic drug penetration through the skin. A novel approach to investigate drug release from topical formulations containing 0.05% clobetasol propionate using in vitro microdialysis was also undertaken. The in vitro findings were found to be in agreement with the results obtained using tape stripping to assess bioequivalence of the same commercially available products, namely Dermovate® cream, Dovate® Cream and Dermovate® ointment. These results indicate the potential to correlate in vitro with in vivo data for bioequivalence assessment of such topical dosage forms.
- Full Text:
- Date Issued: 2010
Isolation and evolution of novel nucleoside phosphorylases
- Authors: Visser, Daniel Finsch
- Date: 2010
- Subjects: AIDS (Disease) -- Treatment -- Africa HIV Infections -- Treatment -- Africa AIDS (Disease) -- Patients -- Africa HIV-Positive persons -- Africa Antiretroviral agents Pyrimidine nucleotides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3972 , http://hdl.handle.net/10962/d1004031
- Description: Approximately 33.4 million people are living with HIV/AIDS. Of those, 97% live in low and middle income countries, with 22.4 million in sub-Saharan Africa. Only 42% of the people who require anti-retrovirals (ARVs) in low to middle income countries are receiving anti-retroviral therapy (ART). There is a need to develop novel and cost effective methods for producing antiretroviral drugs. Stavudine and azidothymidine (AZT) were identified as potential targets because they could both be produced through a common intermediate – 5 methyluridine (5-MU). It has been established that the biocatalytic production of 5-methyluridine is possible through a reaction known as transglycosylation, in a process which has not previously been demonstrated as commercially viable. A selection of biocatalysts were expressed either in recombinant E. coli strains or in the wild type organisms, purified and then screened for their ability to produce 5-MU. A combination of Bacillus halodurans purine nucleoside phosphorylase 1 (BHPNP1) and E. coli uridine phosphorylase (EcUP) gave the highest 5-MU yield (80%). This result represents the first combination of free enzymes from different organisms, giving high yields of 5-MU under high substrate conditions. Both enzymes were purified and successfully characterised. The established pH optimum was pH 7.0 for both enzymes. Temperature optima and stability data for BHPNP1 (70 C and t1/2 at 60 C of 20.8 h) indicated that the biocatalytic step was operating within the capabilities of this enzyme and would operate well at elevated temperatures (up to 60 C). Conversely, the temperature optimum and stability data for EcUP (optimum of 40 C and t1/2 at 60 C of 9.9 h) indicated that the enzyme remained active at 40 C for the duration of a 25 h biotransformation, but at 60 C would only be operating at 20% of its optimum activity and would lose activity rapidly. BHPNP1 and EcUP were used in a bench scale (650 ml) transglycosylation for the production of 5-MU. A 5-MU yield of 79.1% was obtained at this scale with a reactor productivity of 1.37 g.l-1.h-1. Iterative saturation mutagenesis was used to rapidly evolve EcUP for improved thermostability. A moderately high throughput colorimetric method was developed for screening the mutants based on the release of p-nitrophenol upon phosphorolysis of a pyrimidine nucleoside analogue. By screening under 20 000 clones the mutant UPL8 was isolated. The mutant enzyme showed an optimum temperature of 60 C and improved stability at 60 C (t1/2 = 17.3 h). The increase in stability of UPL8 is due to only 2 mutations (Lys235Arg, Gln236Ala). These mutations may have caused an increase in stability due to interactions with other structural units in the protein, stabilization of the entrance to the binding pocket, or by decreasing the flexibility of the α-helix at the N-terminus. Transglycosylation experiments showed that the mutant enzyme UPL8 is a superior catalyst for the production of 5-MU. A 300% increase in reactor productivity was noted when free enzyme preparations of UPL8 was combined with BHPNP1 at 1.5% m.m-1 substrate loading. The high yield of 5-MU (75-80% mol.mol-1) was maintained at 9% m.m-1 substrate loading. A commercially viable productivity of 31 g.l-1.h-1 was thus realised. Further optimisation of the process could produce still higher productivities. Future work in directed evolution of nucleoside phosphorylases is envisaged for improved stability and enhanced substrate range for application to other commercially relevant transglycosylation reactions.
- Full Text:
- Date Issued: 2010
- Authors: Visser, Daniel Finsch
- Date: 2010
- Subjects: AIDS (Disease) -- Treatment -- Africa HIV Infections -- Treatment -- Africa AIDS (Disease) -- Patients -- Africa HIV-Positive persons -- Africa Antiretroviral agents Pyrimidine nucleotides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3972 , http://hdl.handle.net/10962/d1004031
- Description: Approximately 33.4 million people are living with HIV/AIDS. Of those, 97% live in low and middle income countries, with 22.4 million in sub-Saharan Africa. Only 42% of the people who require anti-retrovirals (ARVs) in low to middle income countries are receiving anti-retroviral therapy (ART). There is a need to develop novel and cost effective methods for producing antiretroviral drugs. Stavudine and azidothymidine (AZT) were identified as potential targets because they could both be produced through a common intermediate – 5 methyluridine (5-MU). It has been established that the biocatalytic production of 5-methyluridine is possible through a reaction known as transglycosylation, in a process which has not previously been demonstrated as commercially viable. A selection of biocatalysts were expressed either in recombinant E. coli strains or in the wild type organisms, purified and then screened for their ability to produce 5-MU. A combination of Bacillus halodurans purine nucleoside phosphorylase 1 (BHPNP1) and E. coli uridine phosphorylase (EcUP) gave the highest 5-MU yield (80%). This result represents the first combination of free enzymes from different organisms, giving high yields of 5-MU under high substrate conditions. Both enzymes were purified and successfully characterised. The established pH optimum was pH 7.0 for both enzymes. Temperature optima and stability data for BHPNP1 (70 C and t1/2 at 60 C of 20.8 h) indicated that the biocatalytic step was operating within the capabilities of this enzyme and would operate well at elevated temperatures (up to 60 C). Conversely, the temperature optimum and stability data for EcUP (optimum of 40 C and t1/2 at 60 C of 9.9 h) indicated that the enzyme remained active at 40 C for the duration of a 25 h biotransformation, but at 60 C would only be operating at 20% of its optimum activity and would lose activity rapidly. BHPNP1 and EcUP were used in a bench scale (650 ml) transglycosylation for the production of 5-MU. A 5-MU yield of 79.1% was obtained at this scale with a reactor productivity of 1.37 g.l-1.h-1. Iterative saturation mutagenesis was used to rapidly evolve EcUP for improved thermostability. A moderately high throughput colorimetric method was developed for screening the mutants based on the release of p-nitrophenol upon phosphorolysis of a pyrimidine nucleoside analogue. By screening under 20 000 clones the mutant UPL8 was isolated. The mutant enzyme showed an optimum temperature of 60 C and improved stability at 60 C (t1/2 = 17.3 h). The increase in stability of UPL8 is due to only 2 mutations (Lys235Arg, Gln236Ala). These mutations may have caused an increase in stability due to interactions with other structural units in the protein, stabilization of the entrance to the binding pocket, or by decreasing the flexibility of the α-helix at the N-terminus. Transglycosylation experiments showed that the mutant enzyme UPL8 is a superior catalyst for the production of 5-MU. A 300% increase in reactor productivity was noted when free enzyme preparations of UPL8 was combined with BHPNP1 at 1.5% m.m-1 substrate loading. The high yield of 5-MU (75-80% mol.mol-1) was maintained at 9% m.m-1 substrate loading. A commercially viable productivity of 31 g.l-1.h-1 was thus realised. Further optimisation of the process could produce still higher productivities. Future work in directed evolution of nucleoside phosphorylases is envisaged for improved stability and enhanced substrate range for application to other commercially relevant transglycosylation reactions.
- Full Text:
- Date Issued: 2010
Isomorphism, institutional entrepreneurship and total quality management (TQM) : a case study in the implementation of quality management standards and excellence models in South African developmental local government
- Authors: Naidoo, Pravine
- Date: 2010
- Subjects: Local government -- South Africa -- Eastern Cape Political entrepreneurship -- South Africa -- Eastern Cape Total quality management Municipal services -- South Africa -- Eastern Cape -- Management Municipal services -- Customer services -- South Africa -- Eastern Cape Political leadership -- South Africa -- Eastern Cape Organizational effectiveness
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1193 , http://hdl.handle.net/10962/d1007141
- Description: Within the new constitutional order, South African local government has been afforded specific status and is protected as a distinctive sphere of government in terms of the Constitution Act 108 of 1996. Municipalities are no longer a mere function of national or provincial governments. In addition to this entrenched status, the Constitution and other developmental local government legislation vests local government with specific functions and responsibilities as part of the process of building a new democracy and promoting socio-economic development and upliftment. Consequently, local government managers are under constant pressure to improve the performance and quality of service delivery. They are expected to satisfy a number of stakeholders, achieve increases in efficiency, as well as attain developmental service delivery goals as contained in their Integrated Development Plan (IDP). Quality management standards and excellence models in support of the total quality management (TQM) philosophy are relatively new public management approaches that changes traditional organisational decision-making practices to deliver services to its customers in more effective and efficient ways. In essence, quality management standards and excellence models are performance management methodological practices, techniques and tools designed to meet and exceed public requirements. It accomplishes this through determining what constitutes excellence in customer service, and then empowering employees to a never-ending search for quality improvement in the delivery of services, in terms of the continuous improvement and continuous process improvement philosophies, of which total quality management (TQM) is premised on. Consequently, quality management standards and excellence models (such as the ISO 9001: 2008 QMS, ISO 14001: 2004 EMS, EFQM and SAEF Excellence Models) as methodological practices, techniques or tools in support of the critical factors (CFs) of the total quality management (TQM) philosophy, namely: (1) customer-based approach, (2) senior management commitment and leadership, (3) quality planning, (4) management based on facts, (5) continuous improvement, (6) involvement of all members of the organisation, (7) education and training, (8) teamwork, (9) communication systems, (10) learning, (11) process management, (12) co-operation with suppliers, and (13) organisational awareness and concern for the social and environmental context, can provide a framework by which to achieve the goals of effective and efficient service delivery in developmental local government, as they foster a culture of performance and accountability. Local government managers could use these quality and excellence methodological practices, techniques and tools, as a starting point to improve efficiencies in their municipalities, as the programmes afford them an opportunity to systematically evaluate the municipality and determine what leads to organisational success and deliverables. Institutional theory suggests that organisations are both influenced by and can influence the society in which they operate. Institutional theorists believe that the motivation for a change in internal practices might not only be performance related, but may primarily be to enhance or maintain the legitimacy of the respective institution. Institutional theory also focuses on the social contract that exists between the institution and society. This social contract is believed to represent the expectations of society. From an institutional point of view, it has been suggested that institutions may change and adopt the norms of society to appear legitimate to that society. This infers that when societal norms and values change, institutions will be expected to change. These societal pressures combined with legislative changes, require institutional entrepreneurs to respond to these isomorphic pressures, and will work to preserve the institution's legitimacy by incorporating, or at least appearing to incorporate, new practices, norms and values. The aim of this research study was to analyse within an institutional theory framework, the implementation of quality management standards and excellence models in support of the total quality management (TQM) philosophy and developmental local government within two South African municipalities. The research investigation has been undertaken within the social constructionist paradigm and using the case study method. Interviews and documents were the data sources and thematic analysis was used as a tool to analyse the data by applying a theory-driven coding procedure. The findings from the two research sites, namely Makana Municipality in Grahamstown and Drakenstein Municipality in Paarl reveal that the implementation of quality management standards and excellence models in support of the total quality management (TQM) philosophy in both municipalities was not a smooth process and there were many challenges such as political instability, lack of commitment on the part of the senior leadership and management, staff and skills shortages, resistance by staff to implementation and resource constraints. Institutional theory has proved to be useful in understanding why the Makana and Drakenstein Municipalities implemented quality management standards and excellence models. Because of coercive, mimetic and normative isomorphic pressures affecting the Makana and Drakenstein municipalities, change was imperative if these institutions were to remain relevant and legitimate in order to address its service delivery mandate of effectiveness and efficiency. Institutional theory provided a theoretical lens to understand and appreciate the pressures that affected the municipalities because of its new developmental mandate as encapsulated in new municipal legislation. It also assisted in understanding how institutional entrepreneurs responded to these pressures to conform. The implementation of quality management standards and excellence models in the Makana and Drakenstein municipalities was adversely affected by volatility amongst the political leadership of both municipalities. The successful implementation of these standards and models would require collective action from the political, bureaucratic and procedural entrepreneurs in order to enhance effective, efficient and accountable service delivery. However, in both the Makana and Drakenstein municipalities, institutional entrepreneurs, namely councillors and officials were not working together as a collective to implement quality management standards and excellence models. Evidence has shown that the absence of stable political leadership and noncooperation and collaboration amongst institutional entrepreneurs contributed significantly to the non-institutionalisation of quality management standards and excellence models in these Municipalities. This implies that legitimacy has not been achieved, due to institutional entrepreneurs not collaborating and collectively working with each other to implement the quality management standards and excellence models. Thus, this research study suggests that the strategic and collective role of the political and administrative leadership as institutional entrepreneurs is critical if the municipality is to achieve its aims and objectives, namely effective, efficient, and accountable service delivery in developmental local government. Recommendations in respect of future research and to practitioners have also been offered.
- Full Text:
- Date Issued: 2010
- Authors: Naidoo, Pravine
- Date: 2010
- Subjects: Local government -- South Africa -- Eastern Cape Political entrepreneurship -- South Africa -- Eastern Cape Total quality management Municipal services -- South Africa -- Eastern Cape -- Management Municipal services -- Customer services -- South Africa -- Eastern Cape Political leadership -- South Africa -- Eastern Cape Organizational effectiveness
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1193 , http://hdl.handle.net/10962/d1007141
- Description: Within the new constitutional order, South African local government has been afforded specific status and is protected as a distinctive sphere of government in terms of the Constitution Act 108 of 1996. Municipalities are no longer a mere function of national or provincial governments. In addition to this entrenched status, the Constitution and other developmental local government legislation vests local government with specific functions and responsibilities as part of the process of building a new democracy and promoting socio-economic development and upliftment. Consequently, local government managers are under constant pressure to improve the performance and quality of service delivery. They are expected to satisfy a number of stakeholders, achieve increases in efficiency, as well as attain developmental service delivery goals as contained in their Integrated Development Plan (IDP). Quality management standards and excellence models in support of the total quality management (TQM) philosophy are relatively new public management approaches that changes traditional organisational decision-making practices to deliver services to its customers in more effective and efficient ways. In essence, quality management standards and excellence models are performance management methodological practices, techniques and tools designed to meet and exceed public requirements. It accomplishes this through determining what constitutes excellence in customer service, and then empowering employees to a never-ending search for quality improvement in the delivery of services, in terms of the continuous improvement and continuous process improvement philosophies, of which total quality management (TQM) is premised on. Consequently, quality management standards and excellence models (such as the ISO 9001: 2008 QMS, ISO 14001: 2004 EMS, EFQM and SAEF Excellence Models) as methodological practices, techniques or tools in support of the critical factors (CFs) of the total quality management (TQM) philosophy, namely: (1) customer-based approach, (2) senior management commitment and leadership, (3) quality planning, (4) management based on facts, (5) continuous improvement, (6) involvement of all members of the organisation, (7) education and training, (8) teamwork, (9) communication systems, (10) learning, (11) process management, (12) co-operation with suppliers, and (13) organisational awareness and concern for the social and environmental context, can provide a framework by which to achieve the goals of effective and efficient service delivery in developmental local government, as they foster a culture of performance and accountability. Local government managers could use these quality and excellence methodological practices, techniques and tools, as a starting point to improve efficiencies in their municipalities, as the programmes afford them an opportunity to systematically evaluate the municipality and determine what leads to organisational success and deliverables. Institutional theory suggests that organisations are both influenced by and can influence the society in which they operate. Institutional theorists believe that the motivation for a change in internal practices might not only be performance related, but may primarily be to enhance or maintain the legitimacy of the respective institution. Institutional theory also focuses on the social contract that exists between the institution and society. This social contract is believed to represent the expectations of society. From an institutional point of view, it has been suggested that institutions may change and adopt the norms of society to appear legitimate to that society. This infers that when societal norms and values change, institutions will be expected to change. These societal pressures combined with legislative changes, require institutional entrepreneurs to respond to these isomorphic pressures, and will work to preserve the institution's legitimacy by incorporating, or at least appearing to incorporate, new practices, norms and values. The aim of this research study was to analyse within an institutional theory framework, the implementation of quality management standards and excellence models in support of the total quality management (TQM) philosophy and developmental local government within two South African municipalities. The research investigation has been undertaken within the social constructionist paradigm and using the case study method. Interviews and documents were the data sources and thematic analysis was used as a tool to analyse the data by applying a theory-driven coding procedure. The findings from the two research sites, namely Makana Municipality in Grahamstown and Drakenstein Municipality in Paarl reveal that the implementation of quality management standards and excellence models in support of the total quality management (TQM) philosophy in both municipalities was not a smooth process and there were many challenges such as political instability, lack of commitment on the part of the senior leadership and management, staff and skills shortages, resistance by staff to implementation and resource constraints. Institutional theory has proved to be useful in understanding why the Makana and Drakenstein Municipalities implemented quality management standards and excellence models. Because of coercive, mimetic and normative isomorphic pressures affecting the Makana and Drakenstein municipalities, change was imperative if these institutions were to remain relevant and legitimate in order to address its service delivery mandate of effectiveness and efficiency. Institutional theory provided a theoretical lens to understand and appreciate the pressures that affected the municipalities because of its new developmental mandate as encapsulated in new municipal legislation. It also assisted in understanding how institutional entrepreneurs responded to these pressures to conform. The implementation of quality management standards and excellence models in the Makana and Drakenstein municipalities was adversely affected by volatility amongst the political leadership of both municipalities. The successful implementation of these standards and models would require collective action from the political, bureaucratic and procedural entrepreneurs in order to enhance effective, efficient and accountable service delivery. However, in both the Makana and Drakenstein municipalities, institutional entrepreneurs, namely councillors and officials were not working together as a collective to implement quality management standards and excellence models. Evidence has shown that the absence of stable political leadership and noncooperation and collaboration amongst institutional entrepreneurs contributed significantly to the non-institutionalisation of quality management standards and excellence models in these Municipalities. This implies that legitimacy has not been achieved, due to institutional entrepreneurs not collaborating and collectively working with each other to implement the quality management standards and excellence models. Thus, this research study suggests that the strategic and collective role of the political and administrative leadership as institutional entrepreneurs is critical if the municipality is to achieve its aims and objectives, namely effective, efficient, and accountable service delivery in developmental local government. Recommendations in respect of future research and to practitioners have also been offered.
- Full Text:
- Date Issued: 2010
Justice through language : a critical analysis of the use of foreign African interpreters in South African courtrooms
- Authors: Usadolo, Sam Erevbenagie
- Date: 2010
- Subjects: Translators
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8363 , http://hdl.handle.net/10948/1503 , Translators
- Description: This study represents an analysis of the use of foreign African interpreters in South African courtrooms in the context of the necessity of court interpreting as a vehicle through which accused persons can express themselves in defence of their rights, which may have been violated. As a background to the study, due consideration is given to the history of interpreting, followed by some theoretical perspectives of interpreting, where the differences between translation and interpreting are explained. The discussion of some of the theoretical issues of interpreting also focuses on the notion of equivalence, and the divergent views of scholars regarding this notion, which range from formal equivalence (a source-language oriented approach), through dynamic equivalence (in terms of which translation/interpreting must be in agreement with the form and cultural expectations of the receptor language community) to skopos theory (functional in approach and target text oriented). In discussing the role of the court interpreter, the role boundaries with regard to his/her professional relationship with other participants in the courtroom are highlighted. A related topic, namely that of quality in interpreting, is investigated in relation to quality control by professional associations in different countries, who act as regulatory authorities in this regard. A review of court interpreting in South Africa and selected countries in Africa and the EU, such as Nigeria, Benin, Kenya, Zimbabwe, Namibia, Mozambique and Portugal reveals that efforts to develop court interpreting in some Africa and EU countries are either non-existent or at an incipient stage of development. While South Africa, compared to other countries in Africa, is making progress, she is far behind EU countries such as the UK, France and Spain –all of which possess accreditation systems, professional associations and registers of court interpreters. An analysis of the data collected on foreign African court interpreters shows that, although they work in the same courtrooms as their South African counterparts on a daily basis, they are treated differently in terms of employment procedures, training, remuneration, and such like. The study points out that some factors such as (a) the lack of adequate crosscultural awareness and (b) of a balanced proficiency in their language pair, as well as (c) and the existence of divergent dialects in those languages may pose a challenge to foreign African court interpreters in interpreting cross-border languages and in performing sight translations. The study further reveals that foreign African court interpreters are in serious breach of the professional code of conduct, as exemplified by instances of conflict of interest and partiality in their practices. As regards the management of court interpreters, the findings indicate a general laxity on the side of management, as a result of which there is a lack, inter alia, of an appropriate evaluation or monitoring strategy aimed at detecting such cases of conflict of interest and partiality.
- Full Text:
- Date Issued: 2010
- Authors: Usadolo, Sam Erevbenagie
- Date: 2010
- Subjects: Translators
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8363 , http://hdl.handle.net/10948/1503 , Translators
- Description: This study represents an analysis of the use of foreign African interpreters in South African courtrooms in the context of the necessity of court interpreting as a vehicle through which accused persons can express themselves in defence of their rights, which may have been violated. As a background to the study, due consideration is given to the history of interpreting, followed by some theoretical perspectives of interpreting, where the differences between translation and interpreting are explained. The discussion of some of the theoretical issues of interpreting also focuses on the notion of equivalence, and the divergent views of scholars regarding this notion, which range from formal equivalence (a source-language oriented approach), through dynamic equivalence (in terms of which translation/interpreting must be in agreement with the form and cultural expectations of the receptor language community) to skopos theory (functional in approach and target text oriented). In discussing the role of the court interpreter, the role boundaries with regard to his/her professional relationship with other participants in the courtroom are highlighted. A related topic, namely that of quality in interpreting, is investigated in relation to quality control by professional associations in different countries, who act as regulatory authorities in this regard. A review of court interpreting in South Africa and selected countries in Africa and the EU, such as Nigeria, Benin, Kenya, Zimbabwe, Namibia, Mozambique and Portugal reveals that efforts to develop court interpreting in some Africa and EU countries are either non-existent or at an incipient stage of development. While South Africa, compared to other countries in Africa, is making progress, she is far behind EU countries such as the UK, France and Spain –all of which possess accreditation systems, professional associations and registers of court interpreters. An analysis of the data collected on foreign African court interpreters shows that, although they work in the same courtrooms as their South African counterparts on a daily basis, they are treated differently in terms of employment procedures, training, remuneration, and such like. The study points out that some factors such as (a) the lack of adequate crosscultural awareness and (b) of a balanced proficiency in their language pair, as well as (c) and the existence of divergent dialects in those languages may pose a challenge to foreign African court interpreters in interpreting cross-border languages and in performing sight translations. The study further reveals that foreign African court interpreters are in serious breach of the professional code of conduct, as exemplified by instances of conflict of interest and partiality in their practices. As regards the management of court interpreters, the findings indicate a general laxity on the side of management, as a result of which there is a lack, inter alia, of an appropriate evaluation or monitoring strategy aimed at detecting such cases of conflict of interest and partiality.
- Full Text:
- Date Issued: 2010
Lag length selection for vector error correction models
- Authors: Sharp, Gary David
- Date: 2010
- Subjects: Akaike Information Criterion Mathematical models -- Evaluation Autoregression (Statistics) Error analysis (Mathematics)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5568 , http://hdl.handle.net/10962/d1002808
- Description: This thesis investigates the problem of model identification in a Vector Autoregressive framework. The study reviews the existing research, conducts an extensive simulation based analysis of thirteen information theoretic criterion (IC), one of which is a novel derivation. The simulation exercise considers the evaluation of seven alternative error restricted vector autoregressive models with four different lag lengths. Alternative sample sizes and parameterisations are also evaluated and compared to results in the existing literature. The results of the comparative analysis provide strong support for the efficiency based criterion of Akaike and in particular the selection capability of the novel criterion, referred to as a modified corrected Akaike information criterion, demonstrates useful finite sample properties.
- Full Text:
- Date Issued: 2010
- Authors: Sharp, Gary David
- Date: 2010
- Subjects: Akaike Information Criterion Mathematical models -- Evaluation Autoregression (Statistics) Error analysis (Mathematics)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5568 , http://hdl.handle.net/10962/d1002808
- Description: This thesis investigates the problem of model identification in a Vector Autoregressive framework. The study reviews the existing research, conducts an extensive simulation based analysis of thirteen information theoretic criterion (IC), one of which is a novel derivation. The simulation exercise considers the evaluation of seven alternative error restricted vector autoregressive models with four different lag lengths. Alternative sample sizes and parameterisations are also evaluated and compared to results in the existing literature. The results of the comparative analysis provide strong support for the efficiency based criterion of Akaike and in particular the selection capability of the novel criterion, referred to as a modified corrected Akaike information criterion, demonstrates useful finite sample properties.
- Full Text:
- Date Issued: 2010
Land use, compensational justice and energy resource extraction in Nigeria: a socio-historical study of petroleum and coal mining communities
- Authors: Umejesi, Ikechukwu
- Date: 2010
- Subjects: Compensation (Law) -- Nigeria , Right of property -- Nigeria , Mines and mineral resources -- Nigeria , Coal mines and mining -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD (Sociology)
- Identifier: vital:11950 , http://hdl.handle.net/10353/344 , Compensation (Law) -- Nigeria , Right of property -- Nigeria , Mines and mineral resources -- Nigeria , Coal mines and mining -- Nigeria
- Description: Scholarly and public analyses of state-community conflict in resource-rich communities, especially in Nigeria, often portray the compensational practices of the state and extractive enterprises as unjust and unsustainable. According to this view, at least three issues foreground the “unjustness”, namely: a) Inadequate compensation of land owners when land is expropriated or degraded in the process of natural resource exploration and production; b) inadequate periodic rents paid by extractive firms to land owners; and c) lack of, or inadequate socio-economic infrastructure in the host communities of extractive operations. Most analysts have therefore argued for a revamp of the compensation system and have presented the inadequacy of compensation as the underlying cause of conflict in Nigeria‟s mining communities (see Frynas, 2000b:208; Okoji, 2002:205). This thesis subjects the compensation discourse to a closer examination, especially against the backdrop of underdevelopment, pervasive poverty, environmental damage and continuing corporate-community conflict in Nigeria‟s resource-rich rural communities. The main argument is that, because of some of its underlying neoliberal assumptions, much of the compensation discourse is flawed – which is why the discourse obscures the true character of state-community and corporate-community conflict. This more so, because the discourse relies mainly on post-colonial (that is, post-1960) experiences and contemporary advocacy literature, ignores the interplay between history and contemporary developments in state-community relations, and treats compensation as an independent variable. Drawing on the concept of collective memory, and utilising historical, ethnographic and survey data from two of Nigeria‟s oldest petroleum and coal-mining communities, the thesis examines how the evolution of the Nigerian state and collective memory about aspects of that evolution have shaped state-community relations in the extractive sector. It situates state- iii community resource-related conflict within the wider socio-historical matrix of state and community contestations for ecological and natural resource sovereignty. The key finding of the thesis is that within the context of socio-ecological rights, compensation demands by local communities are textured. In the case of the communities selected for the study, such demands are often made outside, rather than within, local ethnographic ideas of “justness” and “fairness”. Hence, land-related grievances associated with natural resource extraction persist, regardless of whether or not local demands for compensation are “adequately” met by the state and extractive corporations. The thesis enriches and extends our understanding of natural resource conflict by privileging both the sociological and historical contexts of the conflict and raising questions about the dominance the state enjoys over local communities and indigenous ecological spaces.
- Full Text:
- Date Issued: 2010
- Authors: Umejesi, Ikechukwu
- Date: 2010
- Subjects: Compensation (Law) -- Nigeria , Right of property -- Nigeria , Mines and mineral resources -- Nigeria , Coal mines and mining -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD (Sociology)
- Identifier: vital:11950 , http://hdl.handle.net/10353/344 , Compensation (Law) -- Nigeria , Right of property -- Nigeria , Mines and mineral resources -- Nigeria , Coal mines and mining -- Nigeria
- Description: Scholarly and public analyses of state-community conflict in resource-rich communities, especially in Nigeria, often portray the compensational practices of the state and extractive enterprises as unjust and unsustainable. According to this view, at least three issues foreground the “unjustness”, namely: a) Inadequate compensation of land owners when land is expropriated or degraded in the process of natural resource exploration and production; b) inadequate periodic rents paid by extractive firms to land owners; and c) lack of, or inadequate socio-economic infrastructure in the host communities of extractive operations. Most analysts have therefore argued for a revamp of the compensation system and have presented the inadequacy of compensation as the underlying cause of conflict in Nigeria‟s mining communities (see Frynas, 2000b:208; Okoji, 2002:205). This thesis subjects the compensation discourse to a closer examination, especially against the backdrop of underdevelopment, pervasive poverty, environmental damage and continuing corporate-community conflict in Nigeria‟s resource-rich rural communities. The main argument is that, because of some of its underlying neoliberal assumptions, much of the compensation discourse is flawed – which is why the discourse obscures the true character of state-community and corporate-community conflict. This more so, because the discourse relies mainly on post-colonial (that is, post-1960) experiences and contemporary advocacy literature, ignores the interplay between history and contemporary developments in state-community relations, and treats compensation as an independent variable. Drawing on the concept of collective memory, and utilising historical, ethnographic and survey data from two of Nigeria‟s oldest petroleum and coal-mining communities, the thesis examines how the evolution of the Nigerian state and collective memory about aspects of that evolution have shaped state-community relations in the extractive sector. It situates state- iii community resource-related conflict within the wider socio-historical matrix of state and community contestations for ecological and natural resource sovereignty. The key finding of the thesis is that within the context of socio-ecological rights, compensation demands by local communities are textured. In the case of the communities selected for the study, such demands are often made outside, rather than within, local ethnographic ideas of “justness” and “fairness”. Hence, land-related grievances associated with natural resource extraction persist, regardless of whether or not local demands for compensation are “adequately” met by the state and extractive corporations. The thesis enriches and extends our understanding of natural resource conflict by privileging both the sociological and historical contexts of the conflict and raising questions about the dominance the state enjoys over local communities and indigenous ecological spaces.
- Full Text:
- Date Issued: 2010
Leonotis leonurus: the anticoagulant and antidiabetic activity of Leonotis leonurus
- Authors: Mnonopi, Nandipha
- Date: 2010
- Subjects: Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10323 , http://hdl.handle.net/10948/1194 , Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Description: Commercial marrubiin, aqueous and organic extracts of Leonotis leonurus were tested in vitro for their anticoagulant and antiplatelet activities. The aqueous extract inhibited platelet aggregation by 69.5 percent (100 μg/mL), while the organic extract (100 μg/mL) and marrubiin (5 μg/mL) showed 92.5 percent and 91.6 percent inhibition, respectively, by inhibiting the binding of fibrinogen to glycoprotein IIb/IIIa receptor in a concentration dependent manner. The extracts significantly prolonged activated partial thromboplastin time compared to untreated plasma controls. Fibrin and D-Dimer formation were drastically decreased. The extracts and marrubiin concentration-dependently inhibited calcium mobilization induced by collagen and thrombin. The formation of thromboxane A2 was also significantly reduced by both the extracts and marrubiin. Protein secretion and platelet adhesion were significantly reduced by both the extracts and marrubiin. The organic extract and marrubiin showed a more pronounced effect than the aqueous extracts in all the in vitro assays. The ex-vivo animal model confirmed the results obtained in vitro. Similar to the in vitro studies, activated partial thromboplastin time clotting time was prolonged by marrubiin and the number of aggregated platelets were significantly reduced relative to aspirin. The findings reflect that marrubiin largely contributes to the organic extract's anticoagulant and antiplatelet effect in vitro. INS-1 cells were cultured under normo- and hyperglycaemic conditions. Marrubiin and the two Leonotis leonurus extracts were screened for anti-diabetic activity in vitro. The stimulatory index of INS-1 cells cultured under hyperglycaemic conditions was significantly increased by 60 percent and 61 percent (p<0.01; n=5) in cells exposed to the organic extract (10 μg/mL) and marrubiin (500 ng/mL), respectively, relative to the normoglycaemic conditions. The gene expression of insulin was significantly increased by 76.5 and 71 percent, and of glucose transporter-2 by 93 and 92.5 percent for marrubiin and the organic extract, respectively, under the same conditions stipulated above (p<0.01; n=4). The extract and marrubiin similarly showed an increase in respiratory rate under hyperglycaemic conditions. Marrubiin increased insulin secretion, HDL-cholesterol, while it decreased total cholesterol, LDL-cholesterol and the atherogenic index in the in vivo rat model.
- Full Text:
- Date Issued: 2010
- Authors: Mnonopi, Nandipha
- Date: 2010
- Subjects: Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10323 , http://hdl.handle.net/10948/1194 , Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Description: Commercial marrubiin, aqueous and organic extracts of Leonotis leonurus were tested in vitro for their anticoagulant and antiplatelet activities. The aqueous extract inhibited platelet aggregation by 69.5 percent (100 μg/mL), while the organic extract (100 μg/mL) and marrubiin (5 μg/mL) showed 92.5 percent and 91.6 percent inhibition, respectively, by inhibiting the binding of fibrinogen to glycoprotein IIb/IIIa receptor in a concentration dependent manner. The extracts significantly prolonged activated partial thromboplastin time compared to untreated plasma controls. Fibrin and D-Dimer formation were drastically decreased. The extracts and marrubiin concentration-dependently inhibited calcium mobilization induced by collagen and thrombin. The formation of thromboxane A2 was also significantly reduced by both the extracts and marrubiin. Protein secretion and platelet adhesion were significantly reduced by both the extracts and marrubiin. The organic extract and marrubiin showed a more pronounced effect than the aqueous extracts in all the in vitro assays. The ex-vivo animal model confirmed the results obtained in vitro. Similar to the in vitro studies, activated partial thromboplastin time clotting time was prolonged by marrubiin and the number of aggregated platelets were significantly reduced relative to aspirin. The findings reflect that marrubiin largely contributes to the organic extract's anticoagulant and antiplatelet effect in vitro. INS-1 cells were cultured under normo- and hyperglycaemic conditions. Marrubiin and the two Leonotis leonurus extracts were screened for anti-diabetic activity in vitro. The stimulatory index of INS-1 cells cultured under hyperglycaemic conditions was significantly increased by 60 percent and 61 percent (p<0.01; n=5) in cells exposed to the organic extract (10 μg/mL) and marrubiin (500 ng/mL), respectively, relative to the normoglycaemic conditions. The gene expression of insulin was significantly increased by 76.5 and 71 percent, and of glucose transporter-2 by 93 and 92.5 percent for marrubiin and the organic extract, respectively, under the same conditions stipulated above (p<0.01; n=4). The extract and marrubiin similarly showed an increase in respiratory rate under hyperglycaemic conditions. Marrubiin increased insulin secretion, HDL-cholesterol, while it decreased total cholesterol, LDL-cholesterol and the atherogenic index in the in vivo rat model.
- Full Text:
- Date Issued: 2010
Local economic development: a case study of the Blue Crane Route Municipality, Eastern Cape, South Africa
- Authors: Boose, Refuoe
- Date: 2010
- Subjects: Local government -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Rural-urban relations -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Geography)
- Identifier: vital:11509 , http://hdl.handle.net/10353/248 , Local government -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Rural-urban relations -- South Africa -- Eastern Cape
- Description: Local economic development (LED) in the countries of the North is a voluntary activity of local government, in South Africa it is a mandatory activity. The purpose of LED is to build the capacity of a local area to improve its economic future and the quality of life for all. While local governments in South Africa are currently engaged with the concept of LED which is seen as a tool through which to achieve sustainable development, it is now recognized that there exists economic, social and environmental interdependence between rural and urban areas and a need for a balanced and mutually supportive approach to development of the rural and urban areas. Adopting the Rural-Urban, Economic and Enterprise Development (RUEED) concept, this study highlights the challenges and development problems experienced by the deep rural and urban communities in the implementation of LED activities within the Blue Crane Route Municipality (BCRM) due to the lack of social, economic and environmental sustainability and rural-urban linkages. This study located in the Blue Crane Route Municipality in the Eastern Cape, seeks to investigate and recommend a map or strategies linking together the urban communities with the poorer neighbourhoods in LED activities. The intensive research design that incorporates the case study method was used to achieve the objectives of this study. The empirical findings of the research indicate that the concept of rural-urban linkages is not an adopted strategy in implementing development programmes resulting in deep rural communities being excluded in LED activities initiated within the Blue Crane Route Municipality. The findings also reveal that the creation of employment opportunities and improvement in the standard of living are important dimensions of LED and rural-urban linkages. This study therefore recommends the adoption of a new perspective referred to as the rural-urban linkages for LED and the consideration of economic, social and environmental complementaries that exist between rural and urban areas in the Blue Crane Route Municipality. The study further indicates that it is critical that the LED policy and interventions reflect the existing patterns of interactions between the rural and urban areas of the Municipality.
- Full Text:
- Date Issued: 2010
- Authors: Boose, Refuoe
- Date: 2010
- Subjects: Local government -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Rural-urban relations -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Geography)
- Identifier: vital:11509 , http://hdl.handle.net/10353/248 , Local government -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Rural-urban relations -- South Africa -- Eastern Cape
- Description: Local economic development (LED) in the countries of the North is a voluntary activity of local government, in South Africa it is a mandatory activity. The purpose of LED is to build the capacity of a local area to improve its economic future and the quality of life for all. While local governments in South Africa are currently engaged with the concept of LED which is seen as a tool through which to achieve sustainable development, it is now recognized that there exists economic, social and environmental interdependence between rural and urban areas and a need for a balanced and mutually supportive approach to development of the rural and urban areas. Adopting the Rural-Urban, Economic and Enterprise Development (RUEED) concept, this study highlights the challenges and development problems experienced by the deep rural and urban communities in the implementation of LED activities within the Blue Crane Route Municipality (BCRM) due to the lack of social, economic and environmental sustainability and rural-urban linkages. This study located in the Blue Crane Route Municipality in the Eastern Cape, seeks to investigate and recommend a map or strategies linking together the urban communities with the poorer neighbourhoods in LED activities. The intensive research design that incorporates the case study method was used to achieve the objectives of this study. The empirical findings of the research indicate that the concept of rural-urban linkages is not an adopted strategy in implementing development programmes resulting in deep rural communities being excluded in LED activities initiated within the Blue Crane Route Municipality. The findings also reveal that the creation of employment opportunities and improvement in the standard of living are important dimensions of LED and rural-urban linkages. This study therefore recommends the adoption of a new perspective referred to as the rural-urban linkages for LED and the consideration of economic, social and environmental complementaries that exist between rural and urban areas in the Blue Crane Route Municipality. The study further indicates that it is critical that the LED policy and interventions reflect the existing patterns of interactions between the rural and urban areas of the Municipality.
- Full Text:
- Date Issued: 2010