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An analysis of ways in which the South African tax system could be simplified
- Authors: Young, Gail Jeni
- Date: 2021-04
- Subjects: Taxation -- Law and legislation -- South Africa , Income tax -- Law and legislation -- South Africa , South African Revenue Service , Tax administration and procedure -- South Africa , Tax accounting -- South Africa
- Language: English
- Type: thesis , text , Masters , MCom
- Identifier: http://hdl.handle.net/10962/178235 , vital:42923
- Description: It has been said that the fundamental paradox of tax simplification is that, despite consensus, almost every year tax rules become more complex. This thesis considers tax simplification measures which have been implemented internationally, in order to provide a basis for an analysis of ways in which the South African tax system could be simplified. A doctrinal methodology is applied, and an analysis is carried out of possible tax simplification measures, based on the commentary of experts in the field of tax law. Simplification measures adopted in the United Kingdom, Australia, the United States of America, Egypt, and certain European countries are discussed, together with their possible adoption in South Africa. Tax simplification has a broad scope. This research identifies four areas in which the South African tax system could simplified: the simplification of tax legislation, addressing the role of accounting in the simplification process, reducing the number of taxes currently levied, and finally addressing the complexities evident in the SARS e-filing system. This thesis illustrates several measures which could be used to address the current areas of complexity. Re-writing tax legislation to assist the understanding of taxpayers is suggested. An increase in the inclusion rate for individuals of capital gains in taxable income from 40% to 60% is suggested, to compensate for the loss of revenue due to the recommended repeal of donations tax and estate duty. Aligning tax legislation with accounting standards is identified as a possible area for simplification, as there are many similarities between the two systems. To address the usability of SARS’ e-filing platform, suggestions are made regarding the further pre-population of returns, introducing e-invoicing and providing a “sandbox” function that taxpayers could use to familiarise themselves with how e-filing works. This research highlights tax simplification as a process that needs to be prioritized in order to achieve the associated benefits. , Thesis (MCom) -- Faculty of Commerce, Accounting, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Young, Gail Jeni
- Date: 2021-04
- Subjects: Taxation -- Law and legislation -- South Africa , Income tax -- Law and legislation -- South Africa , South African Revenue Service , Tax administration and procedure -- South Africa , Tax accounting -- South Africa
- Language: English
- Type: thesis , text , Masters , MCom
- Identifier: http://hdl.handle.net/10962/178235 , vital:42923
- Description: It has been said that the fundamental paradox of tax simplification is that, despite consensus, almost every year tax rules become more complex. This thesis considers tax simplification measures which have been implemented internationally, in order to provide a basis for an analysis of ways in which the South African tax system could be simplified. A doctrinal methodology is applied, and an analysis is carried out of possible tax simplification measures, based on the commentary of experts in the field of tax law. Simplification measures adopted in the United Kingdom, Australia, the United States of America, Egypt, and certain European countries are discussed, together with their possible adoption in South Africa. Tax simplification has a broad scope. This research identifies four areas in which the South African tax system could simplified: the simplification of tax legislation, addressing the role of accounting in the simplification process, reducing the number of taxes currently levied, and finally addressing the complexities evident in the SARS e-filing system. This thesis illustrates several measures which could be used to address the current areas of complexity. Re-writing tax legislation to assist the understanding of taxpayers is suggested. An increase in the inclusion rate for individuals of capital gains in taxable income from 40% to 60% is suggested, to compensate for the loss of revenue due to the recommended repeal of donations tax and estate duty. Aligning tax legislation with accounting standards is identified as a possible area for simplification, as there are many similarities between the two systems. To address the usability of SARS’ e-filing platform, suggestions are made regarding the further pre-population of returns, introducing e-invoicing and providing a “sandbox” function that taxpayers could use to familiarise themselves with how e-filing works. This research highlights tax simplification as a process that needs to be prioritized in order to achieve the associated benefits. , Thesis (MCom) -- Faculty of Commerce, Accounting, 2021
- Full Text:
- Date Issued: 2021-04
An Archive of Upset : the Shift from Commissioning to Curating through South Africa’s Representations at the Bienal de São Paulo and the Interstitial Nexus of Leonard Tshehla Mohapi Matsoso
- Authors: Dantas, Nancy Isabel
- Date: 2021-04
- Subjects: Bienal Internacional de São Paulo , Curatorship , Art -- Commissioning , Art -- Political aspects , Matsoso, Leonard Tshehla Mohapi
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/174492 , vital:42482 , https://doi.org/10.21504/10962/174492
- Description: This thesis explores the distinction between commissioning and curating, adopting the Bienal de São Paulo (or Bienal) as its conceptual propeller and point of departure. The thesis regards exhibitions as palimpsests, in other words, platforms built on previous conscious or sublimated models, beyond the Venetian model inaugurated in 1895. By looking at world expositions, particularly the Cape presence at the Colonial and Indian Exhibition of 1886, this project traces a lineage of commissioners, from Sir Henry Cole to Sydney Cowper through to the Director of the Pretoria Art Museum, Albert Werth. It distinguishes these men, their vision and allegiances from the curatorial model instantiated in South Africa by the late Okwui Enwezor as a consequence of the Second Johannesburg Biennale, held in 1997. The aim of this research has been to provide a partial but crucial account of this shift, and to remain attentive to the silences and deletions, to what happens in the interstice, at transitionary moments of ‘betweenness.’ I ask that readers consider the 1979 Bienal as an instance of an interstice where the occluded and silenced ghost of modernist artist Leonard Tshehla Mohapi Matsoso, who represented South Africa at the 1973 edition of the Bienal, garnering a substantial award for his work in drawing, resides. Matsoso was the first and only Black South African artist to receive this accolade. This thesis posits that Matsoso’s absence from the exhibition in 1979, an exhibition where he would rightly have featured, constitutes a curatorial haunting, wedged in the archive of the Bienal’s history, and an opportunity for revision and evaluation of commissioning vis-àvis curating. In reading the exhibition histories’ archive “along the grain” (Stoler 2002), the commissioner emerges as a man of letters, a privileged social category found in the archive; a colonial authority whose status was founded as much on his display of European learning as on his studied ignorance of local knowledge; an implementer of the taxonomic state and modernist art historical canon (in the case of Werth); a cultivator of the fine arts of deference, dissemblance and persuasion. At a later stage and moment of dissonance and disruption, the independent curator emerges to reconsider, question and expand the canon, distancing him/herself from the (South African) State to serve the artist or artists and a wider community. This research aims to contribute, albeit in a small way, to a reappraisal of the position of Leonard Tshehla Mohapi Matsoso in South African modernism, and the distinction between commissioning and curating. , Thesis (PhD)--Rhodes University, Faculty of Humanities, Department of Fine Art, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Dantas, Nancy Isabel
- Date: 2021-04
- Subjects: Bienal Internacional de São Paulo , Curatorship , Art -- Commissioning , Art -- Political aspects , Matsoso, Leonard Tshehla Mohapi
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/174492 , vital:42482 , https://doi.org/10.21504/10962/174492
- Description: This thesis explores the distinction between commissioning and curating, adopting the Bienal de São Paulo (or Bienal) as its conceptual propeller and point of departure. The thesis regards exhibitions as palimpsests, in other words, platforms built on previous conscious or sublimated models, beyond the Venetian model inaugurated in 1895. By looking at world expositions, particularly the Cape presence at the Colonial and Indian Exhibition of 1886, this project traces a lineage of commissioners, from Sir Henry Cole to Sydney Cowper through to the Director of the Pretoria Art Museum, Albert Werth. It distinguishes these men, their vision and allegiances from the curatorial model instantiated in South Africa by the late Okwui Enwezor as a consequence of the Second Johannesburg Biennale, held in 1997. The aim of this research has been to provide a partial but crucial account of this shift, and to remain attentive to the silences and deletions, to what happens in the interstice, at transitionary moments of ‘betweenness.’ I ask that readers consider the 1979 Bienal as an instance of an interstice where the occluded and silenced ghost of modernist artist Leonard Tshehla Mohapi Matsoso, who represented South Africa at the 1973 edition of the Bienal, garnering a substantial award for his work in drawing, resides. Matsoso was the first and only Black South African artist to receive this accolade. This thesis posits that Matsoso’s absence from the exhibition in 1979, an exhibition where he would rightly have featured, constitutes a curatorial haunting, wedged in the archive of the Bienal’s history, and an opportunity for revision and evaluation of commissioning vis-àvis curating. In reading the exhibition histories’ archive “along the grain” (Stoler 2002), the commissioner emerges as a man of letters, a privileged social category found in the archive; a colonial authority whose status was founded as much on his display of European learning as on his studied ignorance of local knowledge; an implementer of the taxonomic state and modernist art historical canon (in the case of Werth); a cultivator of the fine arts of deference, dissemblance and persuasion. At a later stage and moment of dissonance and disruption, the independent curator emerges to reconsider, question and expand the canon, distancing him/herself from the (South African) State to serve the artist or artists and a wider community. This research aims to contribute, albeit in a small way, to a reappraisal of the position of Leonard Tshehla Mohapi Matsoso in South African modernism, and the distinction between commissioning and curating. , Thesis (PhD)--Rhodes University, Faculty of Humanities, Department of Fine Art, 2021
- Full Text:
- Date Issued: 2021-04
An assessment of different methods for measuring mammal diversity in two Southern African arid ecosystems.
- Authors: Burt, Chloe
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Biodiversity -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52338 , vital:43591
- Description: A current critical environmental problem is the loss of biodiversity. To allow for adaptive management and effective conservation interventions, reliable data needs to be readily available and effectively collected. To gather reliable data, the most appropriate method must be used. The aim of this thesis was to compare the effectiveness and efficiency of different mammal diversity sampling techniques, including discussing specificities and complementarity of each. I also assessed the limitation of methods in terms of the influence of species traits on detectability. I used standard mammal field methods such as block transects, camera traps, track plates, spotlight surveys, mist nets, acoustic surveys, and live traps for a wide range of mammal taxa, and also included the use of local knowledge in the comparison. I assessed the cost-efficiency of each method by determining the cost per species. The efficiency of each method was assessed by observing when the species accumulation curve reached an asymptote. The effectiveness was determined by the species richness and the taxonomic groups the method detected. My results indicated that block transects were the most efficient and effective method to assess species richness. However, a combination of methods allowed for a more comprehensive survey and my results indicate that block transects and live traps were the best combination. It is important to note, not only the effectiveness and efficiency of methods be assessed, but also their limitations. I therefore also assessed the influence of species traits on the detectability of five methods. The species traits that I tested were body size, diet, activity, and if they lived in groups. On both study areas, body size was the main trait that influenced detectability with most field methods. Diet and activity had varying influence on detectability depending on the method. Group living did not significantly influence detectability. Based on my results, I provide guidelines for choosing the most appropriate survey method for targeted studies on mammals in arid ecosystems as well as guidelines for choosing the most appropriate methods for a terrestrial mammal inventory. Survey methods selection and guidelines provided here can allow for effective monitoring and therefore effective and adaptive management and having reliable and repeatable outcomes from surveys. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Management, 2021
- Full Text: false
- Date Issued: 2021-04
- Authors: Burt, Chloe
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Biodiversity -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52338 , vital:43591
- Description: A current critical environmental problem is the loss of biodiversity. To allow for adaptive management and effective conservation interventions, reliable data needs to be readily available and effectively collected. To gather reliable data, the most appropriate method must be used. The aim of this thesis was to compare the effectiveness and efficiency of different mammal diversity sampling techniques, including discussing specificities and complementarity of each. I also assessed the limitation of methods in terms of the influence of species traits on detectability. I used standard mammal field methods such as block transects, camera traps, track plates, spotlight surveys, mist nets, acoustic surveys, and live traps for a wide range of mammal taxa, and also included the use of local knowledge in the comparison. I assessed the cost-efficiency of each method by determining the cost per species. The efficiency of each method was assessed by observing when the species accumulation curve reached an asymptote. The effectiveness was determined by the species richness and the taxonomic groups the method detected. My results indicated that block transects were the most efficient and effective method to assess species richness. However, a combination of methods allowed for a more comprehensive survey and my results indicate that block transects and live traps were the best combination. It is important to note, not only the effectiveness and efficiency of methods be assessed, but also their limitations. I therefore also assessed the influence of species traits on the detectability of five methods. The species traits that I tested were body size, diet, activity, and if they lived in groups. On both study areas, body size was the main trait that influenced detectability with most field methods. Diet and activity had varying influence on detectability depending on the method. Group living did not significantly influence detectability. Based on my results, I provide guidelines for choosing the most appropriate survey method for targeted studies on mammals in arid ecosystems as well as guidelines for choosing the most appropriate methods for a terrestrial mammal inventory. Survey methods selection and guidelines provided here can allow for effective monitoring and therefore effective and adaptive management and having reliable and repeatable outcomes from surveys. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Management, 2021
- Full Text: false
- Date Issued: 2021-04
An assessment of the wild cyclopia subternata (vleitee) resource
- Authors: Van der Walt, Wouter
- Date: 2021-04
- Subjects: Legumes -- South Africa , Fynbos -- South Africa , Fynbos ecology -- South Africa , Fynbos -- Harvesting -- South Africa , Biodiversity conservation -- South Africa , Traditional ecological knowledge -- South Africa , Wild Cyclopia Subternata (Vleitee) , Honeybush tea industry
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/176874 , vital:42767
- Description: Cyclopia spp. is used for the production of Honeybush tea, an herbal tea which is used in many cases as a substitute for rooibos tea. The wild harvesting of this fynbos plant still brings in up to 85% of the annual crop and as such needs to be carefully monitored in order to avoid over harvesting and the destruction of biodiversity within the Eastern and Western Cape mountain regions of Southern Africa. Due to the concerns of overharvesting and unsuitable use of the wild resource issues of supply security have been raised. There is a lack of formal knowledge relating to the plant, its harvesting, the industry and its economic potential. This study aimed to amend this scarcity by contributing to the knowledge of C. subternata as a wild harvested plant, the physical environment favoured by C. subternata, sustainable use through harvesting of the wild plant and harvesting methods specific to this honeybush species. The research concludes by presenting a guideline for best harvest practices of the wild C. subternata plant. , Thesis (MSc) -- Faculty of Science, Department of Geography, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Van der Walt, Wouter
- Date: 2021-04
- Subjects: Legumes -- South Africa , Fynbos -- South Africa , Fynbos ecology -- South Africa , Fynbos -- Harvesting -- South Africa , Biodiversity conservation -- South Africa , Traditional ecological knowledge -- South Africa , Wild Cyclopia Subternata (Vleitee) , Honeybush tea industry
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/176874 , vital:42767
- Description: Cyclopia spp. is used for the production of Honeybush tea, an herbal tea which is used in many cases as a substitute for rooibos tea. The wild harvesting of this fynbos plant still brings in up to 85% of the annual crop and as such needs to be carefully monitored in order to avoid over harvesting and the destruction of biodiversity within the Eastern and Western Cape mountain regions of Southern Africa. Due to the concerns of overharvesting and unsuitable use of the wild resource issues of supply security have been raised. There is a lack of formal knowledge relating to the plant, its harvesting, the industry and its economic potential. This study aimed to amend this scarcity by contributing to the knowledge of C. subternata as a wild harvested plant, the physical environment favoured by C. subternata, sustainable use through harvesting of the wild plant and harvesting methods specific to this honeybush species. The research concludes by presenting a guideline for best harvest practices of the wild C. subternata plant. , Thesis (MSc) -- Faculty of Science, Department of Geography, 2021
- Full Text:
- Date Issued: 2021-04
An educational intervention on the prevention of catheter-related bloodstream infections in haemodialysis patients in Eswatini
- Authors: Simelane, Delisile
- Date: 2021-04
- Subjects: Grahamstown (South Africa) , Catheterization -- Complications -- Eswatini , Infection Control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51906 , vital:43383
- Description: Nurses are health care professionals whose duty it is to protect patients from acquiring infections while hospitalised in a health care setup. By maintaining an infection-free environment, it is expected that the patient's recovery will be promoted, and high-quality nursing care will be delivered. This also applies to the prevention of catheter-related bloodstream infections (CRBSIs) in haemodialysis patients. As nurses spend most of their nursing time with patients, they should have a good level of understanding of infection prevention and control in health care setups. In Eswatini, central venous catheters (CVCs) are commonly used for vascular access in patients who require haemodialysis. From the latest data available in the country's three largest regional hospitals, the proportion of haemodialysis patients with CVCs hospitalised for CRBSIs was 27% (64/239) January to December 2020. Further, it is unclear whether professional nurses have knowledge on the prevention of CRBSIs. This study aimed to investigate the effect of an educational intervention, based on the Centre for Disease Control (CDC) Guidelines, on professional nurses' knowledge regarding the prevention of CRBSIs in haemodialysis patients in hospitals in Eswatini (previously known as Swaziland). The study used a convenience sample including all professional nurses and nurse managers (N=99) employed at the renal and intensive care units (ICUs) at the five hospitals (Hospitals A-E) in Eswatini where haemolysis is conducted. The study is underpinned by Florence Nightingale's Theory and the Knowledge-to-Action Framework. The research study followed a positivistic, quantitative research paradigm, using a quasi-experimental, one-group, pre-post-test design with an interventional research approach, to be conducted in three phases. The first phase was the distribution of a pre-test questionnaire to all professional nurses and nurse managers working in renal units and those in ICUs that are also managing CVCs in order to assess their knowledge regarding the prevention of CRBSIs in haemodialysis patients. The second phase included the educational intervention, which was developed, reviewed and presented by the researcher to professional nurses. The third phase included the distribution of a post-test questionnaire to the professional nurses to assess the effect of the educational intervention on their knowledge.The pre-and post-test questionnaires were developed by the researcher, in line with the educational intervention (and informed based on the pre-test findings), reviewed by six experts and pilot tested to ascertain its validity and reliability. The researcher captured the data using Microsoft Excel and, with the assistance of a statistician, who performed statistical analyses using Cronbach's alpha, One-sample t-tests, Cohen's Kappa, Cohen's D, and Chi-square test. Ethical principles, according to the Belmont Report, including autonomy, beneficence and justice, and appropriate standards of rigour were maintained throughout the research study. In this study the overall knowledge was good with a mean score of 75.05 ±8.74 for the pre-test and 77.75±9.55 for the post-test. The knowledge scores for the factor infection prevention was higher (pre-test: 83.33±9.28 versus post-test: 85.14±8.28) compared to the factor catheter care (pre-test: 66.76±13.13 versus post-test: 70.38±14.27). The factor catheter care and age was significantly related in the pre-test with a p-value of .043 between groups. The factor infection prevention and how long practising in the unit were significantly related in the pre-test, with a p-value of .029 between groups. The factor infection prevention in the pre-test had a medium significant difference between >1 year and >5 years practicing in the unit (Scheffé p .048 and Cohen's d 0.77). The study is the first in the field of nursing in Eswatini. The educational intervention can be used by nurses in renal units and ICUs to close knowledge-practice gaps that can put patients at higher risk for CRBSIs, but requires further development and testing. , Thesis (MN) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Simelane, Delisile
- Date: 2021-04
- Subjects: Grahamstown (South Africa) , Catheterization -- Complications -- Eswatini , Infection Control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51906 , vital:43383
- Description: Nurses are health care professionals whose duty it is to protect patients from acquiring infections while hospitalised in a health care setup. By maintaining an infection-free environment, it is expected that the patient's recovery will be promoted, and high-quality nursing care will be delivered. This also applies to the prevention of catheter-related bloodstream infections (CRBSIs) in haemodialysis patients. As nurses spend most of their nursing time with patients, they should have a good level of understanding of infection prevention and control in health care setups. In Eswatini, central venous catheters (CVCs) are commonly used for vascular access in patients who require haemodialysis. From the latest data available in the country's three largest regional hospitals, the proportion of haemodialysis patients with CVCs hospitalised for CRBSIs was 27% (64/239) January to December 2020. Further, it is unclear whether professional nurses have knowledge on the prevention of CRBSIs. This study aimed to investigate the effect of an educational intervention, based on the Centre for Disease Control (CDC) Guidelines, on professional nurses' knowledge regarding the prevention of CRBSIs in haemodialysis patients in hospitals in Eswatini (previously known as Swaziland). The study used a convenience sample including all professional nurses and nurse managers (N=99) employed at the renal and intensive care units (ICUs) at the five hospitals (Hospitals A-E) in Eswatini where haemolysis is conducted. The study is underpinned by Florence Nightingale's Theory and the Knowledge-to-Action Framework. The research study followed a positivistic, quantitative research paradigm, using a quasi-experimental, one-group, pre-post-test design with an interventional research approach, to be conducted in three phases. The first phase was the distribution of a pre-test questionnaire to all professional nurses and nurse managers working in renal units and those in ICUs that are also managing CVCs in order to assess their knowledge regarding the prevention of CRBSIs in haemodialysis patients. The second phase included the educational intervention, which was developed, reviewed and presented by the researcher to professional nurses. The third phase included the distribution of a post-test questionnaire to the professional nurses to assess the effect of the educational intervention on their knowledge.The pre-and post-test questionnaires were developed by the researcher, in line with the educational intervention (and informed based on the pre-test findings), reviewed by six experts and pilot tested to ascertain its validity and reliability. The researcher captured the data using Microsoft Excel and, with the assistance of a statistician, who performed statistical analyses using Cronbach's alpha, One-sample t-tests, Cohen's Kappa, Cohen's D, and Chi-square test. Ethical principles, according to the Belmont Report, including autonomy, beneficence and justice, and appropriate standards of rigour were maintained throughout the research study. In this study the overall knowledge was good with a mean score of 75.05 ±8.74 for the pre-test and 77.75±9.55 for the post-test. The knowledge scores for the factor infection prevention was higher (pre-test: 83.33±9.28 versus post-test: 85.14±8.28) compared to the factor catheter care (pre-test: 66.76±13.13 versus post-test: 70.38±14.27). The factor catheter care and age was significantly related in the pre-test with a p-value of .043 between groups. The factor infection prevention and how long practising in the unit were significantly related in the pre-test, with a p-value of .029 between groups. The factor infection prevention in the pre-test had a medium significant difference between >1 year and >5 years practicing in the unit (Scheffé p .048 and Cohen's d 0.77). The study is the first in the field of nursing in Eswatini. The educational intervention can be used by nurses in renal units and ICUs to close knowledge-practice gaps that can put patients at higher risk for CRBSIs, but requires further development and testing. , Thesis (MN) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
An evaluation of synergistic interactions between feruloyl esterases and xylanases during the hydrolysis of various pre-treated agricultural residues
- Authors: Mkabayi, Lithalethu
- Date: 2021-04
- Subjects: Esterases , Xylanases , Hydrolysis , Agricultural wastes -- Recycling , Enzymes , Lignocellulose -- Biodegradation , Escherichia coli , Oligosaccharides , Hydroxycinnamic acids
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178224 , vital:42922 , 10.21504/10962/178224
- Description: Agricultural residues are readily available and inexpensive renewable resources that can be used as raw materials for the production of value-added chemicals. The application of enzymes to facilitate the degradation of agricultural residues has long been considered the most environmentally friendly strategy for converting this material into good quality value-added chemicals. However, agricultural residues are typically lignocellulosic in composition and recalcitrant to enzymatic hydrolysis. Due to this recalcitrant nature, the complete degradation of biomass residues requires the synergistic action of a broad range of enzymes. The development and optimisation of synergistic enzyme cocktails is an effective approach for achieving high hydrolysis efficiency of lignocellulosic biomass. The aim of the current study was to evaluate the synergistic interactions between two termite metagenome-derived feruloyl esterases (FAE6 and FAE5) and endo-xylanases for the production of hydroxycinnamic acids and xylo-oligosaccharides (XOS) from model substrates, and untreated and pre-treated agricultural residues. Firstly, the two fae genes were heterologously expressed in Escherichia coli, and the recombinant enzymes were purified to homogeneity. The biochemical properties of the purified recombinant FAEs and xylanases (XT6 and Xyn11) were then assessed to determine the factors which influenced their activities and to select suitable operating conditions for synergy studies. An optimal protein loading ratio of xylanases to FAEs required to maximise the release of both reducing sugar and ferulic acid (FA) was established using 0.5% (w/v) insoluble wheat arabinoxylan (a model substrate). The enzyme combination of 66% xylanase and 33% FAE (on a protein loading basis) produced the highest amounts of reducing sugars and FA. The enzyme combination of XT6 (GH10 xylanase) and FAE5 or FAE6 liberated the highest amount of FA while a combination of Xyn11 (GH11 xylanase) and FAE5 or FAE6 produced the highest reducing sugar content. The synergistic interactions which were established between the xylanases and FAEs were further investigated using agricultural residues (corn cobs, rice straw and sugarcane bagasse). The three substrates were subjected to hydrothermal and dilute acid pre-treatment prior to synergy studies. It is generally known that, during pre-treatment, many compounds can be produced which may influence enzymatic hydrolysis. The effects of these by-products were assessed and it was found that lignin and its degradation products were the most inhibitory to the FAEs. The optimised enzyme cocktail was then applied to 1% (w/v) of untreated and pre-treated substrates for the efficient production of XOS and hydroxycinnamic acids. A significant improvement in xylanase substrate degradation was observed, especially with the combination of 66% Xyn11 and 33% FAE6 which displayed an improvement in reducing sugars of approximately 1.9-fold and 3.4-fold for hydrothermal and acid pre-treated corn cobs (compared to when Xyn11 was used alone), respectively. The study demonstrated that pre-treatment substantially enhanced the enzymatic hydrolysis of corn cobs and rice straw. Analysis of the hydrolysate product profiles revealed that the optimised enzyme cocktail displayed great potential for releasing XOS with a low degree of polymerisation. In conclusion, this study provided significant insights into the mechanism of synergistic interactions between xylanases and metagenome-derived FAEs during the hydrolysis of various substrates. The study also demonstrated that optimised enzyme cocktails combined with low severity pre-treatment can facilitate the potential use of xylan-rich lignocellulosic biomass for the production of valuable products in the future. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Mkabayi, Lithalethu
- Date: 2021-04
- Subjects: Esterases , Xylanases , Hydrolysis , Agricultural wastes -- Recycling , Enzymes , Lignocellulose -- Biodegradation , Escherichia coli , Oligosaccharides , Hydroxycinnamic acids
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178224 , vital:42922 , 10.21504/10962/178224
- Description: Agricultural residues are readily available and inexpensive renewable resources that can be used as raw materials for the production of value-added chemicals. The application of enzymes to facilitate the degradation of agricultural residues has long been considered the most environmentally friendly strategy for converting this material into good quality value-added chemicals. However, agricultural residues are typically lignocellulosic in composition and recalcitrant to enzymatic hydrolysis. Due to this recalcitrant nature, the complete degradation of biomass residues requires the synergistic action of a broad range of enzymes. The development and optimisation of synergistic enzyme cocktails is an effective approach for achieving high hydrolysis efficiency of lignocellulosic biomass. The aim of the current study was to evaluate the synergistic interactions between two termite metagenome-derived feruloyl esterases (FAE6 and FAE5) and endo-xylanases for the production of hydroxycinnamic acids and xylo-oligosaccharides (XOS) from model substrates, and untreated and pre-treated agricultural residues. Firstly, the two fae genes were heterologously expressed in Escherichia coli, and the recombinant enzymes were purified to homogeneity. The biochemical properties of the purified recombinant FAEs and xylanases (XT6 and Xyn11) were then assessed to determine the factors which influenced their activities and to select suitable operating conditions for synergy studies. An optimal protein loading ratio of xylanases to FAEs required to maximise the release of both reducing sugar and ferulic acid (FA) was established using 0.5% (w/v) insoluble wheat arabinoxylan (a model substrate). The enzyme combination of 66% xylanase and 33% FAE (on a protein loading basis) produced the highest amounts of reducing sugars and FA. The enzyme combination of XT6 (GH10 xylanase) and FAE5 or FAE6 liberated the highest amount of FA while a combination of Xyn11 (GH11 xylanase) and FAE5 or FAE6 produced the highest reducing sugar content. The synergistic interactions which were established between the xylanases and FAEs were further investigated using agricultural residues (corn cobs, rice straw and sugarcane bagasse). The three substrates were subjected to hydrothermal and dilute acid pre-treatment prior to synergy studies. It is generally known that, during pre-treatment, many compounds can be produced which may influence enzymatic hydrolysis. The effects of these by-products were assessed and it was found that lignin and its degradation products were the most inhibitory to the FAEs. The optimised enzyme cocktail was then applied to 1% (w/v) of untreated and pre-treated substrates for the efficient production of XOS and hydroxycinnamic acids. A significant improvement in xylanase substrate degradation was observed, especially with the combination of 66% Xyn11 and 33% FAE6 which displayed an improvement in reducing sugars of approximately 1.9-fold and 3.4-fold for hydrothermal and acid pre-treated corn cobs (compared to when Xyn11 was used alone), respectively. The study demonstrated that pre-treatment substantially enhanced the enzymatic hydrolysis of corn cobs and rice straw. Analysis of the hydrolysate product profiles revealed that the optimised enzyme cocktail displayed great potential for releasing XOS with a low degree of polymerisation. In conclusion, this study provided significant insights into the mechanism of synergistic interactions between xylanases and metagenome-derived FAEs during the hydrolysis of various substrates. The study also demonstrated that optimised enzyme cocktails combined with low severity pre-treatment can facilitate the potential use of xylan-rich lignocellulosic biomass for the production of valuable products in the future. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-04
An exploration into an ESD-based teaching strategy to improve the critical thinking and problem-solving skills amongst development studies students at Nelson Mandela University
- Authors: Abrahams, André John
- Date: 2021-04
- Subjects: Problem solving -- Study and teaching , Divergent thinking -- Study and teaching , Sustainable development -- Study and teaching , Environmental education
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/50907 , vital:43148
- Description: In recent years, Education for Sustainable Development (ESD) has been a significant focus for research pertaining to teacher education and transformative approaches to teaching and learning. However, there has been less specific focus on the implementation of ESD in the context of higher education. This study, therefore, explored the use of an ESD-based teaching and learning approach in an undergraduate Development Studies programme. The focus was on developing a teaching approach that was informed by the principles of ESD and which could assist Development Studies lecturers in creating a more transformative, student-centred environment. This study was a qualitative undertaking, conducted at the Nelson Mandela University’s (NMU) Development Studies undergraduate programme. The ESD-based approach was implemented by two participating lecturers who utilised the approach across four undergraduate classes. Qualitative data were generated through a systematic literature analysis (SLA), exploratory interviews, student exercises and assessments, focus group discussions, and reflective journal entries. The gathered data were then analysed using a framework analysis rubric adapted from the theoretical framework utilised in the study. In addition, the data were coded using the six coding techniques promoted by Saldaña (2013). The findings suggest that the ESD-based approach developed in the study could effectively contribute towards promoting ESD in the Development Studies programme. The findings also suggest that, when properly implemented, the ESD-based approach could assist lecturers in creating a more transformative learning environment where students have greater control over their learning experience. In addition, this study found that students in the selected Development Studies undergraduate programme could benefit from continued exposure to the teaching methods and techniques adopted in the approach. The implementation of the approach also uncovered some of the inhibiting factors that may prevent an ESD-based approach being mainstreamed into the context of higher education. , Thesis (DPhil) -- Faculty of Business and Economic Sciences, Development Studies, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Abrahams, André John
- Date: 2021-04
- Subjects: Problem solving -- Study and teaching , Divergent thinking -- Study and teaching , Sustainable development -- Study and teaching , Environmental education
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/50907 , vital:43148
- Description: In recent years, Education for Sustainable Development (ESD) has been a significant focus for research pertaining to teacher education and transformative approaches to teaching and learning. However, there has been less specific focus on the implementation of ESD in the context of higher education. This study, therefore, explored the use of an ESD-based teaching and learning approach in an undergraduate Development Studies programme. The focus was on developing a teaching approach that was informed by the principles of ESD and which could assist Development Studies lecturers in creating a more transformative, student-centred environment. This study was a qualitative undertaking, conducted at the Nelson Mandela University’s (NMU) Development Studies undergraduate programme. The ESD-based approach was implemented by two participating lecturers who utilised the approach across four undergraduate classes. Qualitative data were generated through a systematic literature analysis (SLA), exploratory interviews, student exercises and assessments, focus group discussions, and reflective journal entries. The gathered data were then analysed using a framework analysis rubric adapted from the theoretical framework utilised in the study. In addition, the data were coded using the six coding techniques promoted by Saldaña (2013). The findings suggest that the ESD-based approach developed in the study could effectively contribute towards promoting ESD in the Development Studies programme. The findings also suggest that, when properly implemented, the ESD-based approach could assist lecturers in creating a more transformative learning environment where students have greater control over their learning experience. In addition, this study found that students in the selected Development Studies undergraduate programme could benefit from continued exposure to the teaching methods and techniques adopted in the approach. The implementation of the approach also uncovered some of the inhibiting factors that may prevent an ESD-based approach being mainstreamed into the context of higher education. , Thesis (DPhil) -- Faculty of Business and Economic Sciences, Development Studies, 2021
- Full Text:
- Date Issued: 2021-04
An exploration into an ESD-based teaching strategy to improve the critical thinking and problem-solving skills amongst development studies students at Nelson Mandela University
- Authors: Abrahams, Andre John
- Date: 2021-04
- Subjects: Sustainable development -- Study and teaching , Universities and colleges -- South Africa , Critical thinking
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/50906 , vital:43149
- Description: In recent years, Education for Sustainable Development (ESD) has been a significant focus for research pertaining to teacher education and transformative approaches to teaching and learning. However, there has been less specific focus on the implementation of ESD in the context of higher education. This study, therefore, explored the use of an ESD-based teaching and learning approach in an undergraduate Development Studies programme. The focus was on developing a teaching approach that was informed by the principles of ESD and which could assist Development Studies lecturers in creating a more transformative, student-centred environment. This study was a qualitative undertaking, conducted at the Nelson Mandela University’s (NMU) Development Studies undergraduate programme. The ESD-based approach was implemented by two participating lecturers who utilised the approach across four undergraduate classes. Qualitative data were generated through a systematic literature analysis (SLA), exploratory interviews, student exercises and assessments, focus group discussions, and reflective journal entries. The gathered data were then analysed using a framework analysis rubric adapted from the theoretical framework utilised in the study. In addition, the data were coded using the six coding techniques promoted by Saldaña (2013). The findings suggest that the ESD-based approach developed in the study could effectively contribute towards promoting ESD in the Development Studies programme. The findings also suggest that, when properly implemented, the ESD-based approach could assist lecturers in creating a more transformative learning environment where students have greater control over their learning experience. In addition, this study found that students in the selected Development Studies undergraduate programme could benefit from continued exposure to the teaching methods and techniques adopted in the approach. The implementation of the approach also uncovered some of the inhibiting factors that may prevent an ESD-based approach being mainstreamed into the context of higher education. , Thesis (DPhil) -- Faculty of Business and Economic Sciences, Development Studies, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Abrahams, Andre John
- Date: 2021-04
- Subjects: Sustainable development -- Study and teaching , Universities and colleges -- South Africa , Critical thinking
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/50906 , vital:43149
- Description: In recent years, Education for Sustainable Development (ESD) has been a significant focus for research pertaining to teacher education and transformative approaches to teaching and learning. However, there has been less specific focus on the implementation of ESD in the context of higher education. This study, therefore, explored the use of an ESD-based teaching and learning approach in an undergraduate Development Studies programme. The focus was on developing a teaching approach that was informed by the principles of ESD and which could assist Development Studies lecturers in creating a more transformative, student-centred environment. This study was a qualitative undertaking, conducted at the Nelson Mandela University’s (NMU) Development Studies undergraduate programme. The ESD-based approach was implemented by two participating lecturers who utilised the approach across four undergraduate classes. Qualitative data were generated through a systematic literature analysis (SLA), exploratory interviews, student exercises and assessments, focus group discussions, and reflective journal entries. The gathered data were then analysed using a framework analysis rubric adapted from the theoretical framework utilised in the study. In addition, the data were coded using the six coding techniques promoted by Saldaña (2013). The findings suggest that the ESD-based approach developed in the study could effectively contribute towards promoting ESD in the Development Studies programme. The findings also suggest that, when properly implemented, the ESD-based approach could assist lecturers in creating a more transformative learning environment where students have greater control over their learning experience. In addition, this study found that students in the selected Development Studies undergraduate programme could benefit from continued exposure to the teaching methods and techniques adopted in the approach. The implementation of the approach also uncovered some of the inhibiting factors that may prevent an ESD-based approach being mainstreamed into the context of higher education. , Thesis (DPhil) -- Faculty of Business and Economic Sciences, Development Studies, 2021
- Full Text:
- Date Issued: 2021-04
An exploration of attrition of sexual crime cases of child victims from the perspectives of the police officers, prosecutors and advocates in Kouga District, Eastern Cape (SA)
- Authors: Calitz, Karen
- Date: 2021-04
- Subjects: Sex crimes -- Investigation -- South Africa -- Eastern Cape , Criminal justice, Administration of -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51010 , vital:43200
- Description: The successful prosecution of a sex crime case is dependent on the testimony of the victim. Due to the concealed nature of a sexual crime, it is notoriously difficult to prosecute effectively. With child victims, a number of additional obstacles present themselves when it comes to children giving evidence. Attrition is the rate at which cases do not proceed to court. Statistics in South Africa has revealed that rape has one of the lowest conviction rates of all serious crimes. This study is aimed at analysing the factors that cause attrition during the investigation stage in cases where children were victims of any form of sexual crime. In order to obtain the unscripted view of the investigation process, the qualitative study comprises of interviews with investigating officers who investigate these cases and the prosecutors who present these cases to court. The multi-disciplinary team-approach during investigation and its varying limits and challenges, were the main focus. A purposive non-probability sampling technique was utilised, focusing on the Kouga district municipality of the Eastern Cape. Through this study, the factors causing attrition were analysed and presented in such a manner so that change can be conceptualised in order to strive for the rights of children to receive justice for crimes committed against them. Key findings of the study yielded three significant factors which plague the progress of these cases. A significant finding illuminated the lack of required capacity professionals have in working and understanding children throughout the criminal justice process. A further vital finding is the complexities of inter-departmental functioning on implementation level, indicating challenges in collaboration. An integral finding is the measure of time taken to investigate cases of child victims, which the criminal process flow is delayed to its own detriment in aiming for convictions. In general it was found that services to child victims are neglected. Collaborative approaches which are already in place are to be strengthened through capacity building and collaborative knowledge integration in all sectors of professionals. Victim services legislation must include specialized services for the child victim. , Thesis (MSW) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Calitz, Karen
- Date: 2021-04
- Subjects: Sex crimes -- Investigation -- South Africa -- Eastern Cape , Criminal justice, Administration of -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51010 , vital:43200
- Description: The successful prosecution of a sex crime case is dependent on the testimony of the victim. Due to the concealed nature of a sexual crime, it is notoriously difficult to prosecute effectively. With child victims, a number of additional obstacles present themselves when it comes to children giving evidence. Attrition is the rate at which cases do not proceed to court. Statistics in South Africa has revealed that rape has one of the lowest conviction rates of all serious crimes. This study is aimed at analysing the factors that cause attrition during the investigation stage in cases where children were victims of any form of sexual crime. In order to obtain the unscripted view of the investigation process, the qualitative study comprises of interviews with investigating officers who investigate these cases and the prosecutors who present these cases to court. The multi-disciplinary team-approach during investigation and its varying limits and challenges, were the main focus. A purposive non-probability sampling technique was utilised, focusing on the Kouga district municipality of the Eastern Cape. Through this study, the factors causing attrition were analysed and presented in such a manner so that change can be conceptualised in order to strive for the rights of children to receive justice for crimes committed against them. Key findings of the study yielded three significant factors which plague the progress of these cases. A significant finding illuminated the lack of required capacity professionals have in working and understanding children throughout the criminal justice process. A further vital finding is the complexities of inter-departmental functioning on implementation level, indicating challenges in collaboration. An integral finding is the measure of time taken to investigate cases of child victims, which the criminal process flow is delayed to its own detriment in aiming for convictions. In general it was found that services to child victims are neglected. Collaborative approaches which are already in place are to be strengthened through capacity building and collaborative knowledge integration in all sectors of professionals. Victim services legislation must include specialized services for the child victim. , Thesis (MSW) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
An exploration of spirit possession and psychological praxis in South Africa: implications for a culturally competent psychological practice
- Authors: Yew-Siong, Lauren Cindy
- Date: 2021-04
- Subjects: Spirit possession -- South Africa -- Psychological aspects , Cultural competence -- South Africa -- Psychological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52394 , vital:43635
- Description: Spirit possession has been documented in human history and evidence of it, still exists globally. In South Africa, spirit possession experiences are not uncommon; in some instances, culturally, they can be expressed as ‘amafufunyana’, while religiously, the term demonic/spirit possession holds true. The symptoms of a spirit possession state, should be of interest to psychology, given the similarity of its symptoms with those that are often typical of a psychological diagnosis, such as dissociation, hallucinations and delusions. While much attention has been given to patient experiences of spirit possession, little attention has been focused on the cultural competence required in working with patients with diverse worldviews. This study explores psychologists perceived cultural competence in working with patients who present with the experience of being possessed by a spirit. This exploration becomes more interesting, given that little to no attention in the literature has focused on the role and influence of psychologists religious and cultural belief systems and how these may influence (if at all), the understanding, diagnosis and treatment of a patient who believes that they are possessed. The researcher employed a qualitative method and design. Participants were recruited through use of a purposive sampling technique. Twelve psychologists agreed to participate in the study and thematic analysis was used to analyze the data, by following a data analysis method by Braun and Clarke (2006). Four themes were identified namely, 1) Psychologists’ understanding and treatment of dissociative disorders/states, 2) Psychologists’ understanding of spirit possession; 3) Spirit possession experiences versus psychological disturbances; and 4) Cultural acknowledgement versus cultural considerations-implications for cultural competence. Findings from the study suggest that it is not only cultural considerations that is Running Head: An Exploration of Spirit Possession and Psychological Praxis in South Africa: Implications for a Culturally Competent Psychological Practice. xv needed within psychological practice, but that a sense of cultural acknowledgement is needed in order to bring about potential holistic healing, and more specifically, aid cultural competence within psychological practice. This, according to the findings of the study, can be done through collaboration between various professionals or practitioners- furthermore creating a platform for the inclusion of pluralism within mental healthcare services. , Thesis (MA) -- Faculty of Health Sciences, 2021
- Full Text: false
- Date Issued: 2021-04
- Authors: Yew-Siong, Lauren Cindy
- Date: 2021-04
- Subjects: Spirit possession -- South Africa -- Psychological aspects , Cultural competence -- South Africa -- Psychological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52394 , vital:43635
- Description: Spirit possession has been documented in human history and evidence of it, still exists globally. In South Africa, spirit possession experiences are not uncommon; in some instances, culturally, they can be expressed as ‘amafufunyana’, while religiously, the term demonic/spirit possession holds true. The symptoms of a spirit possession state, should be of interest to psychology, given the similarity of its symptoms with those that are often typical of a psychological diagnosis, such as dissociation, hallucinations and delusions. While much attention has been given to patient experiences of spirit possession, little attention has been focused on the cultural competence required in working with patients with diverse worldviews. This study explores psychologists perceived cultural competence in working with patients who present with the experience of being possessed by a spirit. This exploration becomes more interesting, given that little to no attention in the literature has focused on the role and influence of psychologists religious and cultural belief systems and how these may influence (if at all), the understanding, diagnosis and treatment of a patient who believes that they are possessed. The researcher employed a qualitative method and design. Participants were recruited through use of a purposive sampling technique. Twelve psychologists agreed to participate in the study and thematic analysis was used to analyze the data, by following a data analysis method by Braun and Clarke (2006). Four themes were identified namely, 1) Psychologists’ understanding and treatment of dissociative disorders/states, 2) Psychologists’ understanding of spirit possession; 3) Spirit possession experiences versus psychological disturbances; and 4) Cultural acknowledgement versus cultural considerations-implications for cultural competence. Findings from the study suggest that it is not only cultural considerations that is Running Head: An Exploration of Spirit Possession and Psychological Praxis in South Africa: Implications for a Culturally Competent Psychological Practice. xv needed within psychological practice, but that a sense of cultural acknowledgement is needed in order to bring about potential holistic healing, and more specifically, aid cultural competence within psychological practice. This, according to the findings of the study, can be done through collaboration between various professionals or practitioners- furthermore creating a platform for the inclusion of pluralism within mental healthcare services. , Thesis (MA) -- Faculty of Health Sciences, 2021
- Full Text: false
- Date Issued: 2021-04
An exploration of working with grade 11 life sciences educators on the use of Virtual Lab to mediate learning of energy transformations
- Authors: Shambare, Brian
- Date: 2021-04
- Subjects: Life sciences -- Study and teaching (Secondary) -- South Africa -- Eastern Cape , Energy conversion -- Study and teaching , Educational technology -- South Africa -- Eastern Cape , Teaching -- Aids and devices , Virtual Lab
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/174388 , vital:42473
- Description: The Department of Basic Education (DBE) examiners’ diagnostic reports for 2012-2019 indicate that National Senior Certificate (NSC) learners mostly perform poorly in examination questions based on scientific investigations. The low performance by learners in these questions has been attributed to the failure by teachers to effectively mediate the learning of scientific concepts due to lack of science laboratories or poorly resourced laboratories in most rural schools. As a result, most learners are finding that scientific concepts are decontextualized and hence abstract. Thus, this study explored making use of Virtual Lab to mediate learning of scientific investigations using the topic Energy transformations. The study was located within an interpretive paradigm and a qualitative case study approach was employed. The study was conducted in four different rural schools in the Joe Gqabi district and seven Grade 11 Life Sciences teachers participated. Data was generated using semi-structured questionnaires, semi-structured interviews, lesson observation, workshop discussions, and journal reflections. The study was informed by Vygotsky’s (1978) Socio-Cultural Theory (SCT) as the theoretical framework, and Thompson and Mishra’s (2006) Technological Pedagogical Content Knowledge (TPACK) as the analytical framework. The results of this study showed that most educators have a positive predisposition towards the integration of Information and Communication Technologies (ICT) in their practice. The study found that using the Virtual Lab to teach Life Sciences has several benefits such as safe environment for conducting experiments; convenience and accessibility; positive teacher and learner attitudes and improvement on learner performance; elimination of physical limitations of a real lab; and availability top-class lab equipment and up-to-date reagents. The study also revealed some shortcomings that were associated with the use of the Virtual Lab. These are; lack of lab partner and peer-learning; and lack of direct supervision by a more knowledgeable facilitator. This study concluded that using the Virtual Lab enhances the quality of teaching scientific experiments in the selected under-resourced rural secondary schools. The study recommends the adoption of the Virtual Lab as a viable alternative to the conventional lab.
- Full Text:
- Date Issued: 2021-04
- Authors: Shambare, Brian
- Date: 2021-04
- Subjects: Life sciences -- Study and teaching (Secondary) -- South Africa -- Eastern Cape , Energy conversion -- Study and teaching , Educational technology -- South Africa -- Eastern Cape , Teaching -- Aids and devices , Virtual Lab
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/174388 , vital:42473
- Description: The Department of Basic Education (DBE) examiners’ diagnostic reports for 2012-2019 indicate that National Senior Certificate (NSC) learners mostly perform poorly in examination questions based on scientific investigations. The low performance by learners in these questions has been attributed to the failure by teachers to effectively mediate the learning of scientific concepts due to lack of science laboratories or poorly resourced laboratories in most rural schools. As a result, most learners are finding that scientific concepts are decontextualized and hence abstract. Thus, this study explored making use of Virtual Lab to mediate learning of scientific investigations using the topic Energy transformations. The study was located within an interpretive paradigm and a qualitative case study approach was employed. The study was conducted in four different rural schools in the Joe Gqabi district and seven Grade 11 Life Sciences teachers participated. Data was generated using semi-structured questionnaires, semi-structured interviews, lesson observation, workshop discussions, and journal reflections. The study was informed by Vygotsky’s (1978) Socio-Cultural Theory (SCT) as the theoretical framework, and Thompson and Mishra’s (2006) Technological Pedagogical Content Knowledge (TPACK) as the analytical framework. The results of this study showed that most educators have a positive predisposition towards the integration of Information and Communication Technologies (ICT) in their practice. The study found that using the Virtual Lab to teach Life Sciences has several benefits such as safe environment for conducting experiments; convenience and accessibility; positive teacher and learner attitudes and improvement on learner performance; elimination of physical limitations of a real lab; and availability top-class lab equipment and up-to-date reagents. The study also revealed some shortcomings that were associated with the use of the Virtual Lab. These are; lack of lab partner and peer-learning; and lack of direct supervision by a more knowledgeable facilitator. This study concluded that using the Virtual Lab enhances the quality of teaching scientific experiments in the selected under-resourced rural secondary schools. The study recommends the adoption of the Virtual Lab as a viable alternative to the conventional lab.
- Full Text:
- Date Issued: 2021-04
An exploratory investigation into an Integrated Vulnerability and Patch Management Framework
- Authors: Carstens, Duane
- Date: 2021-04
- Subjects: Computer security , Computer security -- Management , Computer networks -- Security measures , Patch Management , Integrated Vulnerability
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/177940 , vital:42892
- Description: In the rapidly changing world of cybersecurity, the constant increase of vulnerabilities continues to be a prevalent issue for many organisations. Malicious actors are aware that most organisations cannot timeously patch known vulnerabilities and are ill-prepared to protect against newly created vulnerabilities where a signature or an available patch has not yet been created. Consequently, information security personnel face ongoing challenges to mitigate these risks. In this research, the problem of remediation in a world of increasing vulnerabilities is considered. The current paradigm of vulnerability and patch management is reviewed using a pragmatic approach to all associated variables of these services / practices and, as a result, what is working and what is not working in terms of remediation is understood. In addition to the analysis, a taxonomy is created to provide a graphical representation of all associated variables to vulnerability and patch management based on existing literature. Frameworks currently being utilised in the industry to create an effective engagement model between vulnerability and patch management services are considered. The link between quantifying a threat, vulnerability and consequence; what Microsoft has available for patching; and the action plan for resulting vulnerabilities is explored. Furthermore, the processes and means of communication between each of these services are investigated to ensure there is effective remediation of vulnerabilities, ultimately improving the security risk posture of an organisation. In order to effectively measure the security risk posture, progress is measured between each of these services through a single averaged measurement metric. The outcome of the research highlights influencing factors that impact successful vulnerability management, in line with identified themes from the research taxonomy. These influencing factors are however significantly undermined due to resources within the same organisations not having a clear and consistent understanding of their role, organisational capabilities and objectives for effective vulnerability and patch management within their organisations. , Thesis (MSc) -- Faculty of Science, Computer Science, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Carstens, Duane
- Date: 2021-04
- Subjects: Computer security , Computer security -- Management , Computer networks -- Security measures , Patch Management , Integrated Vulnerability
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/177940 , vital:42892
- Description: In the rapidly changing world of cybersecurity, the constant increase of vulnerabilities continues to be a prevalent issue for many organisations. Malicious actors are aware that most organisations cannot timeously patch known vulnerabilities and are ill-prepared to protect against newly created vulnerabilities where a signature or an available patch has not yet been created. Consequently, information security personnel face ongoing challenges to mitigate these risks. In this research, the problem of remediation in a world of increasing vulnerabilities is considered. The current paradigm of vulnerability and patch management is reviewed using a pragmatic approach to all associated variables of these services / practices and, as a result, what is working and what is not working in terms of remediation is understood. In addition to the analysis, a taxonomy is created to provide a graphical representation of all associated variables to vulnerability and patch management based on existing literature. Frameworks currently being utilised in the industry to create an effective engagement model between vulnerability and patch management services are considered. The link between quantifying a threat, vulnerability and consequence; what Microsoft has available for patching; and the action plan for resulting vulnerabilities is explored. Furthermore, the processes and means of communication between each of these services are investigated to ensure there is effective remediation of vulnerabilities, ultimately improving the security risk posture of an organisation. In order to effectively measure the security risk posture, progress is measured between each of these services through a single averaged measurement metric. The outcome of the research highlights influencing factors that impact successful vulnerability management, in line with identified themes from the research taxonomy. These influencing factors are however significantly undermined due to resources within the same organisations not having a clear and consistent understanding of their role, organisational capabilities and objectives for effective vulnerability and patch management within their organisations. , Thesis (MSc) -- Faculty of Science, Computer Science, 2021
- Full Text:
- Date Issued: 2021-04
An investigation into the current state of web based cryptominers and cryptojacking
- Authors: Len, Robert
- Date: 2021-04
- Subjects: Cryptocurrencies , Malware (Computer software) , Computer networks -- Security measures , Computer networks -- Monitoring , Cryptomining , Coinhive , Cryptojacking
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/178248 , vital:42924
- Description: The aim of this research was to conduct a review of the current state and extent of surreptitious crypto mining software and its prevalence as a means for income generation. Income is generated through the use of a viewer's browser to execute custom JavaScript code to mine cryptocurrencies such as Monero and Bitcoin. The research aimed to measure the prevalence of illicit mining scripts being utilised for “in-browser" cryptojacking while further analysing the ecosystems that support the cryptomining environment. The extent of the research covers aspects such as the content (or type) of the sites hosting malicious “in-browser" cryptomining software as well as the occurrences of currencies utilised in the cryptographic mining and the analysis of cryptographic mining code samples. This research aims to compare the results of previous work with the current state of affairs since the closure of Coinhive in March 2018. Coinhive were at the time the market leader in such web based mining services. Beyond the analysis of the prevalence of cryptomining on the web today, research into the methodologies and techniques used to detect and counteract cryptomining are also conducted. This includes the most recent developments in malicious JavaScript de-obfuscation as well as cryptomining signature creation and detection. Methodologies for heuristic JavaScript behaviour identification and subsequent identification of potential malicious out-liars are also included within the research of the countermeasure analysis. The research revealed that although no longer functional, Coinhive remained as the most prevalent script being used for “in-browser" cryptomining services. While remaining the most prevalent, there was however a significant decline in overall occurrences compared to when coinhive.com was operational. Analysis of the ecosystem hosting \in-browser" mining websites was found to be distributed both geographically as well as in terms of domain categorisations. , Thesis (MSc) -- Faculty of Science, Computer Science, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Len, Robert
- Date: 2021-04
- Subjects: Cryptocurrencies , Malware (Computer software) , Computer networks -- Security measures , Computer networks -- Monitoring , Cryptomining , Coinhive , Cryptojacking
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/178248 , vital:42924
- Description: The aim of this research was to conduct a review of the current state and extent of surreptitious crypto mining software and its prevalence as a means for income generation. Income is generated through the use of a viewer's browser to execute custom JavaScript code to mine cryptocurrencies such as Monero and Bitcoin. The research aimed to measure the prevalence of illicit mining scripts being utilised for “in-browser" cryptojacking while further analysing the ecosystems that support the cryptomining environment. The extent of the research covers aspects such as the content (or type) of the sites hosting malicious “in-browser" cryptomining software as well as the occurrences of currencies utilised in the cryptographic mining and the analysis of cryptographic mining code samples. This research aims to compare the results of previous work with the current state of affairs since the closure of Coinhive in March 2018. Coinhive were at the time the market leader in such web based mining services. Beyond the analysis of the prevalence of cryptomining on the web today, research into the methodologies and techniques used to detect and counteract cryptomining are also conducted. This includes the most recent developments in malicious JavaScript de-obfuscation as well as cryptomining signature creation and detection. Methodologies for heuristic JavaScript behaviour identification and subsequent identification of potential malicious out-liars are also included within the research of the countermeasure analysis. The research revealed that although no longer functional, Coinhive remained as the most prevalent script being used for “in-browser" cryptomining services. While remaining the most prevalent, there was however a significant decline in overall occurrences compared to when coinhive.com was operational. Analysis of the ecosystem hosting \in-browser" mining websites was found to be distributed both geographically as well as in terms of domain categorisations. , Thesis (MSc) -- Faculty of Science, Computer Science, 2021
- Full Text:
- Date Issued: 2021-04
An investigation into the role of lesson study in developing teachers’ mathematics content and pedagogical content knowledge
- Authors: Kgothego, Naomi Ntsae
- Date: 2021-04
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa -- Bojanala Platinum District Municipality , Pedagogical content knowledge , Lesson planning -- South Africa -- Bojanala Platinum District Municipality , Mathematics teachers -- Training of -- South Africa -- Bojanala Platinum District Municipality , South Africa. Department of Basic Education , Kokusai Kyōryoku Jigyōdan
- Language: English
- Type: thesis , text , Masters , MEd
- Identifier: http://hdl.handle.net/10962/178019 , vital:42899
- Description: Results of international assessments conducted with South African learners, both in primary and secondary schools, suggest that South African learners underperform in mathematics (Spaull, 2013). While there are numerous explanations for this, one of the key explanations is that teachers are deemed to have inadequate knowledge of both mathematics content and pedagogy. Poor content and pedagogical knowledge are indications that teachers are not adequately trained to teach mathematics (Green, 2011). To improve teachers' content and pedagogical knowledge, well-planned and researched professional development programmes need to be put in place. Current professional development opportunities that centre on workshops are not working as they provide little opportunity for teachers to connect the workshop content to the contexts in which they teach. Through a collaboration between the Department of Basic Education (DBE) and Japan International Corporation Agency (JICA), the Lesson Study approach is being introduced to support teachers' professional development. This research seeks to research this approach within the context of Foundation Phase mathematics education. The research asks: How does Lesson Study contribute to the development of teachers' mathematics content and pedagogical content knowledge? Two sub-questions were developed to support the main question: • What mathematics content knowledge do teachers develop as the engage in LS? • What pedagogical content knowledge do teachers develop as they engage in LS? Using a qualitative interpretivist case study approach, I worked collaboratively with four Grade 1 teachers from two schools. Data was generated through observations, semi-structured interviews and document analysis as we engaged in the Lesson Study process. The Mathematics Knowledge for Teaching (Ball, Thames & Phelps, 2008) and the Knowledge Quartet (Rowland, Turner, Thwaites & Huckstep, 2009) frameworks were used as analytic and explanatory tools in this research. This study's findings showed that participation in the interactive cycles of Lesson Study developed the teachers’ confidence, their pedagogical content knowledge and skills and provided them with the opportunity to collaborate and reflect on their knowledge. The study's findings suggest that lesson study can be used as a strategy for improving teacher professional development. , Thesis (MEd) -- Faculty of Education, Education, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Kgothego, Naomi Ntsae
- Date: 2021-04
- Subjects: Mathematics -- Study and teaching (Elementary) -- South Africa -- Bojanala Platinum District Municipality , Pedagogical content knowledge , Lesson planning -- South Africa -- Bojanala Platinum District Municipality , Mathematics teachers -- Training of -- South Africa -- Bojanala Platinum District Municipality , South Africa. Department of Basic Education , Kokusai Kyōryoku Jigyōdan
- Language: English
- Type: thesis , text , Masters , MEd
- Identifier: http://hdl.handle.net/10962/178019 , vital:42899
- Description: Results of international assessments conducted with South African learners, both in primary and secondary schools, suggest that South African learners underperform in mathematics (Spaull, 2013). While there are numerous explanations for this, one of the key explanations is that teachers are deemed to have inadequate knowledge of both mathematics content and pedagogy. Poor content and pedagogical knowledge are indications that teachers are not adequately trained to teach mathematics (Green, 2011). To improve teachers' content and pedagogical knowledge, well-planned and researched professional development programmes need to be put in place. Current professional development opportunities that centre on workshops are not working as they provide little opportunity for teachers to connect the workshop content to the contexts in which they teach. Through a collaboration between the Department of Basic Education (DBE) and Japan International Corporation Agency (JICA), the Lesson Study approach is being introduced to support teachers' professional development. This research seeks to research this approach within the context of Foundation Phase mathematics education. The research asks: How does Lesson Study contribute to the development of teachers' mathematics content and pedagogical content knowledge? Two sub-questions were developed to support the main question: • What mathematics content knowledge do teachers develop as the engage in LS? • What pedagogical content knowledge do teachers develop as they engage in LS? Using a qualitative interpretivist case study approach, I worked collaboratively with four Grade 1 teachers from two schools. Data was generated through observations, semi-structured interviews and document analysis as we engaged in the Lesson Study process. The Mathematics Knowledge for Teaching (Ball, Thames & Phelps, 2008) and the Knowledge Quartet (Rowland, Turner, Thwaites & Huckstep, 2009) frameworks were used as analytic and explanatory tools in this research. This study's findings showed that participation in the interactive cycles of Lesson Study developed the teachers’ confidence, their pedagogical content knowledge and skills and provided them with the opportunity to collaborate and reflect on their knowledge. The study's findings suggest that lesson study can be used as a strategy for improving teacher professional development. , Thesis (MEd) -- Faculty of Education, Education, 2021
- Full Text:
- Date Issued: 2021-04
An investigation into the use of a consumer incentive to encourage recycling in South Africa
- Authors: Conway, John Charles
- Date: 2021-04
- Subjects: Recycling (Waste, etc.) -- Economic aspects , Consumption (Economics) -- South Africa , Consumer behavior
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51096 , vital:43208
- Description: The focus of this study is the factors that influence recycling behaviours, particularly whether incentives are likely to change consumers’ recycling behaviours in South Africa. Africa is said to become the most polluted continent within a few decades. Three of the largest metropoles in South African are likely to run out of landfill space within the next ten years. Waste streams provide considerable opportunity if waste can be safely collected and either reused, recycled, or recovered, but extracting value from the waste streams is costly and difficult. Consumers need to separate waste at source to improve the efficacy of the recycling stream, yet South Africans do not separate waste at source as a norm. The question that arises then is would an incentive improve South Africans’ willingness to recycle? This study tried to determine if incentives are likely to change consumers’ recycling behaviours in South Africa. It explored the relationship between factors that influence recycling behaviours grounded in Taylor and Todd's (1995) extension of Ajzen's (1991) Theory Of Planned Behaviour. A quantitative survey design, using both online and face to face questionnaires, was used to collect data from a cross section of randomly selected consumers. The research determines that the call to address waste management infrastructure and facilities, is indeed correct. It further confirms previous research findings that an incentive alone is not enough to change recycling behaviours as it will not negate other environmental factors. Lower income groups are more conscientious about recycling and may be enticed to participate in the recycling industry through an incentive to improve their income. , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business Administration, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Conway, John Charles
- Date: 2021-04
- Subjects: Recycling (Waste, etc.) -- Economic aspects , Consumption (Economics) -- South Africa , Consumer behavior
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51096 , vital:43208
- Description: The focus of this study is the factors that influence recycling behaviours, particularly whether incentives are likely to change consumers’ recycling behaviours in South Africa. Africa is said to become the most polluted continent within a few decades. Three of the largest metropoles in South African are likely to run out of landfill space within the next ten years. Waste streams provide considerable opportunity if waste can be safely collected and either reused, recycled, or recovered, but extracting value from the waste streams is costly and difficult. Consumers need to separate waste at source to improve the efficacy of the recycling stream, yet South Africans do not separate waste at source as a norm. The question that arises then is would an incentive improve South Africans’ willingness to recycle? This study tried to determine if incentives are likely to change consumers’ recycling behaviours in South Africa. It explored the relationship between factors that influence recycling behaviours grounded in Taylor and Todd's (1995) extension of Ajzen's (1991) Theory Of Planned Behaviour. A quantitative survey design, using both online and face to face questionnaires, was used to collect data from a cross section of randomly selected consumers. The research determines that the call to address waste management infrastructure and facilities, is indeed correct. It further confirms previous research findings that an incentive alone is not enough to change recycling behaviours as it will not negate other environmental factors. Lower income groups are more conscientious about recycling and may be enticed to participate in the recycling industry through an incentive to improve their income. , Thesis (MBA) -- Faculty of Business and Economic Sciences, Business Administration, 2021
- Full Text:
- Date Issued: 2021-04
An investigation of support services available for black female owners of bed & breakfasts in Makhanda
- Authors: Mdluli, Lukhona Silubonile
- Date: 2021-04
- Subjects: Bed and breakfast accommodations -- South Africa -- Makhanda , Businesspeople, Black -- South Africa -- Makhanda , Business enterprises, Black -- South Africa -- Makhanda , Women-owned business enterprises -- South Africa -- Makhanda , Government aid to small business -- South Africa , Small business -- South Africa -- Makhanda , Racism -- South Africa -- Makhanda , Businesswomen -- South Africa -- Makhanda
- Language: English
- Type: thesis , text , Masters , MBA
- Identifier: http://hdl.handle.net/10962/177181 , vital:42797
- Description: Over the last 26 years, the South African government has committed to SMME development. To do this, policies and support institutions were put in place which would create a favourable environment for SMME development. This was particularly important as after 1994, when the South African economy was re-integrated into global markets after years of having sanctions imposed against the country as a result of the apartheid system. The tourism industry was identified as one of the key economic drivers which would strengthen the economy and assist in job creation, especially since South Africa would be able to attract international tourists. Further, it created the opportunity for new participants to enter the industry, including black entrepreneurs who previously could not enter the industry as a result of the apartheid laws. The opening up of the country to international markets and the focus on the tourism industry also presented the opportunity for new forms of tourism to be explored including township tourism, which gave rise to the black-owned bed & breakfast sector. Over time, it has become evident that black entrepreneurs in this sector are facing a number of challenges, in spite of the pro-SMME policies and support institutions that are in place. Because this study seeks to investigate the support services available for black female bed & breakfast owners in Makhanda, a qualitative research approach using the interpretative theoretical framework was applied. By using semi-structured interview questions, to establish what challenges do black female bed and breakfast owners face the researcher was able to respond to the following; what participants identify as their needs; and whether they are able to access the support services that are made available by the Government. Results indicated that the majority of entrepreneurs have difficulty accessing funding to develop their businesses. The seasonality of Grahamstown/ Makhanda tourism businesses has its pros and cons for tourism entrepreneurs. Despite efforts by government to transform the tourism industry, black female entrepreneurs in this industry still experience a myriad of challenges, including passive racism. Insufficient institutional support and inaccessible support services for women have made it difficult to manage in this this environment. Thus, a recommendation is put forward for female focused entrepreneurship policy, which is more responsive in terms of a judicial and legal system(s) aimed at addressing women’s particular needs and challenges. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Mdluli, Lukhona Silubonile
- Date: 2021-04
- Subjects: Bed and breakfast accommodations -- South Africa -- Makhanda , Businesspeople, Black -- South Africa -- Makhanda , Business enterprises, Black -- South Africa -- Makhanda , Women-owned business enterprises -- South Africa -- Makhanda , Government aid to small business -- South Africa , Small business -- South Africa -- Makhanda , Racism -- South Africa -- Makhanda , Businesswomen -- South Africa -- Makhanda
- Language: English
- Type: thesis , text , Masters , MBA
- Identifier: http://hdl.handle.net/10962/177181 , vital:42797
- Description: Over the last 26 years, the South African government has committed to SMME development. To do this, policies and support institutions were put in place which would create a favourable environment for SMME development. This was particularly important as after 1994, when the South African economy was re-integrated into global markets after years of having sanctions imposed against the country as a result of the apartheid system. The tourism industry was identified as one of the key economic drivers which would strengthen the economy and assist in job creation, especially since South Africa would be able to attract international tourists. Further, it created the opportunity for new participants to enter the industry, including black entrepreneurs who previously could not enter the industry as a result of the apartheid laws. The opening up of the country to international markets and the focus on the tourism industry also presented the opportunity for new forms of tourism to be explored including township tourism, which gave rise to the black-owned bed & breakfast sector. Over time, it has become evident that black entrepreneurs in this sector are facing a number of challenges, in spite of the pro-SMME policies and support institutions that are in place. Because this study seeks to investigate the support services available for black female bed & breakfast owners in Makhanda, a qualitative research approach using the interpretative theoretical framework was applied. By using semi-structured interview questions, to establish what challenges do black female bed and breakfast owners face the researcher was able to respond to the following; what participants identify as their needs; and whether they are able to access the support services that are made available by the Government. Results indicated that the majority of entrepreneurs have difficulty accessing funding to develop their businesses. The seasonality of Grahamstown/ Makhanda tourism businesses has its pros and cons for tourism entrepreneurs. Despite efforts by government to transform the tourism industry, black female entrepreneurs in this industry still experience a myriad of challenges, including passive racism. Insufficient institutional support and inaccessible support services for women have made it difficult to manage in this this environment. Thus, a recommendation is put forward for female focused entrepreneurship policy, which is more responsive in terms of a judicial and legal system(s) aimed at addressing women’s particular needs and challenges. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2021
- Full Text:
- Date Issued: 2021-04
Anisotropic copper oxide nanorods decorated with gold and palladium nanoparticles and their enzymatic properties
- Authors: Sicwetsha, Simbongile
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/178547 , vital:42949
- Description: Access restricted until April 2023. , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Sicwetsha, Simbongile
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/178547 , vital:42949
- Description: Access restricted until April 2023. , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-04
Antifungal activity of silver nanoparticles on Cryptococcus species
- Authors: Roman, Nasstasja
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Cryptococcus
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52089 , vital:43445
- Description: Cryptococcus neoformans is responsible for cryptococcal infections, which have become a great concern particularly in immunocompromised patients. This pulmonary disease causes damage to lung tissue and is presumed to be responsible for approximately 650,000 deaths annually. There is a major need for novel antimicrobial agents to avoid the emergence of resistant strains due to extended use of known antifungal drugs. Silver has become an important antimicrobial agent used in medical devices, cosmetics and even house-hold or clothing products. It is highly toxic and has a reduced risk of resistance. Silver nanoparticles have been broadly explored as an antimicrobial agent due to its high reactivity, large surface area and small size. They have been reported to display antimicrobial activity towards bacteria, viruses and fungi. C. neoformans is a pathogenic microorganism that possesses a number of virulence factors which increases the infectivity of the microorganism in immunocompromised individuals. Some of these include the presence of a polysaccharide capsule, melanin, urease and phospholipase production. Owing to limited availability of antifungal drugs and the possibility of drug resistance, this study focused on an alternative antimicrobial agent, namely silver nanoparticles, and the effects that silver nanoparticles had on fungal β-glucan, chitin and urease, phospholipase and melanin virulence factors. Results from our study show that silver nanoparticles inhibited the growth of C. neoformans at concentrations between 3.125 ƒM and 25 ƒM. Both 26.4 nm and 28.4 nm nanoparticles displayed growth inhibition, but were unable to kill the organism at concentrations of 200 ƒM and above. The silver nanoparticles caused a reduction in βglucan content. There was also an accumulation of intracellular ROS, and phospholipase activity, was reduced. In contrast, the organism displayed an increase in chitin content, melanin and urease activity upon exposure to the nanoparticles. This study shows that chemically synthesized silver nanoparticles are an effective antifungal alternative that greatly reduces or inhibits C. neoformans growth at very low concentrations (ƒM). For future studies it may be feasible to investigate the use of these nanoparticles in combination with known antifungal agents, to prevent the emergence of resistance and inhibit possible defense mechanisms displayed by the organism. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2021
- Full Text: false
- Date Issued: 2021-04
- Authors: Roman, Nasstasja
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Cryptococcus
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52089 , vital:43445
- Description: Cryptococcus neoformans is responsible for cryptococcal infections, which have become a great concern particularly in immunocompromised patients. This pulmonary disease causes damage to lung tissue and is presumed to be responsible for approximately 650,000 deaths annually. There is a major need for novel antimicrobial agents to avoid the emergence of resistant strains due to extended use of known antifungal drugs. Silver has become an important antimicrobial agent used in medical devices, cosmetics and even house-hold or clothing products. It is highly toxic and has a reduced risk of resistance. Silver nanoparticles have been broadly explored as an antimicrobial agent due to its high reactivity, large surface area and small size. They have been reported to display antimicrobial activity towards bacteria, viruses and fungi. C. neoformans is a pathogenic microorganism that possesses a number of virulence factors which increases the infectivity of the microorganism in immunocompromised individuals. Some of these include the presence of a polysaccharide capsule, melanin, urease and phospholipase production. Owing to limited availability of antifungal drugs and the possibility of drug resistance, this study focused on an alternative antimicrobial agent, namely silver nanoparticles, and the effects that silver nanoparticles had on fungal β-glucan, chitin and urease, phospholipase and melanin virulence factors. Results from our study show that silver nanoparticles inhibited the growth of C. neoformans at concentrations between 3.125 ƒM and 25 ƒM. Both 26.4 nm and 28.4 nm nanoparticles displayed growth inhibition, but were unable to kill the organism at concentrations of 200 ƒM and above. The silver nanoparticles caused a reduction in βglucan content. There was also an accumulation of intracellular ROS, and phospholipase activity, was reduced. In contrast, the organism displayed an increase in chitin content, melanin and urease activity upon exposure to the nanoparticles. This study shows that chemically synthesized silver nanoparticles are an effective antifungal alternative that greatly reduces or inhibits C. neoformans growth at very low concentrations (ƒM). For future studies it may be feasible to investigate the use of these nanoparticles in combination with known antifungal agents, to prevent the emergence of resistance and inhibit possible defense mechanisms displayed by the organism. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2021
- Full Text: false
- Date Issued: 2021-04
Antiproliferative activity of novel rhenium complexes and medicinal plant extracts
- Oosthuizen, Kenneth Thomas, Venables, Luanne
- Authors: Oosthuizen, Kenneth Thomas , Venables, Luanne
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Medicinal plants -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52028 , vital:43441
- Description: Cancer is a complex, multifactorial disease that affects millions of individuals every year. The adverse side effects and escalating costs of current therapies coupled with the increased incidence of resistance to these therapies make it imperative that we explore novel treatments for the disease. This study investigated two avenues for novel drug design namely, novel synthetic compounds and medicinal plant extracts. The benzimidazole ring system has shown potential as a scaffold for designing novel anticancer agents. Conjugation of the metal rhenium to novel variants of this ring system open up the possibility of designing novel drugs that serve both a diagnostic and a therapeutic function. This study investigated the in vitro anticancer potential of ten such complexes against selected breast and cervical cancer cell lines. The selectivity of the complexes for cancer cells over normal cells was also investigated while the mechanism of action of effective complexes was determined by exploring cell cycle arrest, biochemical markers of apoptosis and mitochondrial membrane disruption. All ten complexes were screened against MCF7 breast and HeLa cervical cancer cell lines with four showing antiproliferative activity against both cancer cell lines and one showing cell line specific toxicity against MCF7 breast cancer cells. When looking at the SAR of the compounds it was noted that activity was higher in compounds which contain two potentially bidentate benzothiazole ligands, while compounds with potentially tridentate ligands show good activity, but only if the benzothiazole moiety is not involved in coordination. The complexes showed greater antiproliferative activity against the MCF7 breast cancer cells with IC50 values ranging from 3.2 to 7.0 µM versus a range of 7.6 to 24.4 µM being obtained on the HeLa cervical cancer cells. Antiproliferative complexes were tested against confluent and log phase Vero cells at their respective IC50 values to determine their effect on “normal” cells. Confluent Vero cells showed less cell death than those in log phase indicating that the complexes show preference for proliferating cells. The mechanism of action of the complexes was studied on both cancer cell lines via cell cycle analysis and apoptosis assays investigating phosphatidylserine translocation, caspase -3 and -8 activation and mitochondrial membrane potential with most complexes inducing cell cycle arrest followed by cell death via both the extrinsic and intrinsic pathways of apoptosis. Since 1940, 49% of all the available anticancer drugs approved for cancer treatment were natural products or directly derived from natural products. Plants are an excellent source of secondary metabolites, many of which are unique chemical compounds that cannot be synthesized in a laboratory. Ethnobotanical surveys conducted in conjunction with Traditional Health Practitioners of the Mkuranga and Same districts in Tanzania identified 25 plants that are used for the treatment of cancer. Four of these plants (A. mossambicensis, C. adenocaule, C. pseudopulchelusis and R. natalensis) with ethnobotanically reported anticancer usage showed cytotoxic activity against brine shrimp and were selected for further in vitro anticancer studies. All four plants were found to have antiproliferative activity against HeLa cervical cancer cells with IC50 values ranging from 3.4 to 50.8 µg/mL. This study was also tasked with investigating the mechanism of action of C. pseudopulchelus on HeLa cervical cancer cells by exploring cell cycle arrest, biochemical markers of apoptosis and mitochondrial membrane x disruption. C. pseudopulchelus caused early M phase arrest followed by slippage and subsequent cell death via the extrinsic pathway of apoptosis. In conclusion, this study showed that both novel complexes as well as medicinal plant extracts represent an interesting avenue for the development of novel anticancer drugs that are cheaper and produce less side effects than current therapies. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2021
- Full Text: false
- Date Issued: 2021-04
- Authors: Oosthuizen, Kenneth Thomas , Venables, Luanne
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Medicinal plants -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52028 , vital:43441
- Description: Cancer is a complex, multifactorial disease that affects millions of individuals every year. The adverse side effects and escalating costs of current therapies coupled with the increased incidence of resistance to these therapies make it imperative that we explore novel treatments for the disease. This study investigated two avenues for novel drug design namely, novel synthetic compounds and medicinal plant extracts. The benzimidazole ring system has shown potential as a scaffold for designing novel anticancer agents. Conjugation of the metal rhenium to novel variants of this ring system open up the possibility of designing novel drugs that serve both a diagnostic and a therapeutic function. This study investigated the in vitro anticancer potential of ten such complexes against selected breast and cervical cancer cell lines. The selectivity of the complexes for cancer cells over normal cells was also investigated while the mechanism of action of effective complexes was determined by exploring cell cycle arrest, biochemical markers of apoptosis and mitochondrial membrane disruption. All ten complexes were screened against MCF7 breast and HeLa cervical cancer cell lines with four showing antiproliferative activity against both cancer cell lines and one showing cell line specific toxicity against MCF7 breast cancer cells. When looking at the SAR of the compounds it was noted that activity was higher in compounds which contain two potentially bidentate benzothiazole ligands, while compounds with potentially tridentate ligands show good activity, but only if the benzothiazole moiety is not involved in coordination. The complexes showed greater antiproliferative activity against the MCF7 breast cancer cells with IC50 values ranging from 3.2 to 7.0 µM versus a range of 7.6 to 24.4 µM being obtained on the HeLa cervical cancer cells. Antiproliferative complexes were tested against confluent and log phase Vero cells at their respective IC50 values to determine their effect on “normal” cells. Confluent Vero cells showed less cell death than those in log phase indicating that the complexes show preference for proliferating cells. The mechanism of action of the complexes was studied on both cancer cell lines via cell cycle analysis and apoptosis assays investigating phosphatidylserine translocation, caspase -3 and -8 activation and mitochondrial membrane potential with most complexes inducing cell cycle arrest followed by cell death via both the extrinsic and intrinsic pathways of apoptosis. Since 1940, 49% of all the available anticancer drugs approved for cancer treatment were natural products or directly derived from natural products. Plants are an excellent source of secondary metabolites, many of which are unique chemical compounds that cannot be synthesized in a laboratory. Ethnobotanical surveys conducted in conjunction with Traditional Health Practitioners of the Mkuranga and Same districts in Tanzania identified 25 plants that are used for the treatment of cancer. Four of these plants (A. mossambicensis, C. adenocaule, C. pseudopulchelusis and R. natalensis) with ethnobotanically reported anticancer usage showed cytotoxic activity against brine shrimp and were selected for further in vitro anticancer studies. All four plants were found to have antiproliferative activity against HeLa cervical cancer cells with IC50 values ranging from 3.4 to 50.8 µg/mL. This study was also tasked with investigating the mechanism of action of C. pseudopulchelus on HeLa cervical cancer cells by exploring cell cycle arrest, biochemical markers of apoptosis and mitochondrial membrane x disruption. C. pseudopulchelus caused early M phase arrest followed by slippage and subsequent cell death via the extrinsic pathway of apoptosis. In conclusion, this study showed that both novel complexes as well as medicinal plant extracts represent an interesting avenue for the development of novel anticancer drugs that are cheaper and produce less side effects than current therapies. , Thesis (MSc) -- Faculty of Science, School of Biomolecular and Chemical Sciences, 2021
- Full Text: false
- Date Issued: 2021-04
Assessing community engagement programmes to mitigate uncontrolled, deliberately ignited fires in forestry plantation-communal land interface, Kwazulu-Natal, South Africa
- Authors: Ramantswana, Nontethelelo
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Forest fires -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52008 , vital:43439
- Description: Forestry plantations in South Africa are surrounded by poor local communities that normally depend on the forestry resources for subsistence. This interface area is faced with human induced uncontrolled fires. There is a lack of understanding regarding the reasons for deliberate ignition of uncontrolled fires. There is also lack of information on the success of community engagement programs (CEPs) as fire protection intervention and / or their implementation barriers. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Management, 2021
- Full Text: false
- Date Issued: 2021-04
- Authors: Ramantswana, Nontethelelo
- Date: 2021-04
- Subjects: Gqeberha (South Africa) , Eastern Cape (South Africa) , Forest fires -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/52008 , vital:43439
- Description: Forestry plantations in South Africa are surrounded by poor local communities that normally depend on the forestry resources for subsistence. This interface area is faced with human induced uncontrolled fires. There is a lack of understanding regarding the reasons for deliberate ignition of uncontrolled fires. There is also lack of information on the success of community engagement programs (CEPs) as fire protection intervention and / or their implementation barriers. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Management, 2021
- Full Text: false
- Date Issued: 2021-04