Baculovirus-based strategies for the management of insect pests: a focus on development and application in South Africa
- Knox, Caroline M, Moore, Sean D, Luke, Garry, Hill, Martin P
- Authors: Knox, Caroline M , Moore, Sean D , Luke, Garry , Hill, Martin P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/416829 , vital:71389 , xlink:href="https://doi.org/10.1080/09583157.2014.949222"
- Description: There is growing concern among governments, scientists, agricultural practitioners and the general public regarding the negative implications of widespread synthetic chemical pesticide application for the control of crop pests. As a result, baculovirus biopesticides are gaining popularity as components of integrated pest management (IPM) programmes in many countries despite several disadvantages related to slow speed of kill, limited host range and complex large scale production. In South Africa, baculoviruses are incorporated into IPM programmes for the control of crop pests in the field, and recent bioprospecting has led to the characterisation of several novel isolates with the potential to be formulated as commercial products. This contribution will provide an overview of the use of baculoviruses against insect pests in South Africa, as well as research and development efforts aimed at broadening their application as biocontrol agents. Challenges faced by researchers in developmental projects as well as potential users of baculoviruses as biopesticides in the field are also discussed.
- Full Text:
- Date Issued: 2015
- Authors: Knox, Caroline M , Moore, Sean D , Luke, Garry , Hill, Martin P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/416829 , vital:71389 , xlink:href="https://doi.org/10.1080/09583157.2014.949222"
- Description: There is growing concern among governments, scientists, agricultural practitioners and the general public regarding the negative implications of widespread synthetic chemical pesticide application for the control of crop pests. As a result, baculovirus biopesticides are gaining popularity as components of integrated pest management (IPM) programmes in many countries despite several disadvantages related to slow speed of kill, limited host range and complex large scale production. In South Africa, baculoviruses are incorporated into IPM programmes for the control of crop pests in the field, and recent bioprospecting has led to the characterisation of several novel isolates with the potential to be formulated as commercial products. This contribution will provide an overview of the use of baculoviruses against insect pests in South Africa, as well as research and development efforts aimed at broadening their application as biocontrol agents. Challenges faced by researchers in developmental projects as well as potential users of baculoviruses as biopesticides in the field are also discussed.
- Full Text:
- Date Issued: 2015
Beauveria and Metarhizium against false codling moth (Lepidoptera: Tortricidae): a step towards selecting isolates for potential development of a mycoinsecticide
- Coombes, Candice A, Hill, Martin P, Moore, Sean D, Dames, Joanna F, Fullard, T
- Authors: Coombes, Candice A , Hill, Martin P , Moore, Sean D , Dames, Joanna F , Fullard, T
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/405757 , vital:70203 , xlink:href="https://hdl.handle.net/10520/EJC167505"
- Description: False codling moth, Thaumatotibia leucotreta Meyrick (1912) (Lepidoptera: Tortricidae), can cause both pre- and post-harvest damage to citrus fruit. Not only can this result in reduced crop yield, but more importantly because of the moth's endemism to sub-Saharan Africa, it is classified as a phytosanitary pest by many export markets. An entire consignment of citrus may be rejected in the presence of a single moth (Moore 2012). Since the bulk of citrus fruit production in South Africa is exported, the control of T. leucotreta is critical (Citrus Growers Association, South Africa 2012). Traditionally, control has been achieved through the use of chemical insecticides; however, residue restrictions, resistance development and concerns about environmental pollution have substantially reduced the dependence on chemical pesticides in citrus. Research on T. leucotreta control has therefore focused on the use of biological organisms (e.g. parasitoids and viruses), which are used as control agents within an integrated pest management (IPM) programme in citrus. These biological control agents, however, only targeted the aboveground life stages of the pest, not the soil-dwelling life stages (late fifth instars, prepupae, pupae), which is the subject of this contribution (Moore 2012).
- Full Text:
- Date Issued: 2015
- Authors: Coombes, Candice A , Hill, Martin P , Moore, Sean D , Dames, Joanna F , Fullard, T
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/405757 , vital:70203 , xlink:href="https://hdl.handle.net/10520/EJC167505"
- Description: False codling moth, Thaumatotibia leucotreta Meyrick (1912) (Lepidoptera: Tortricidae), can cause both pre- and post-harvest damage to citrus fruit. Not only can this result in reduced crop yield, but more importantly because of the moth's endemism to sub-Saharan Africa, it is classified as a phytosanitary pest by many export markets. An entire consignment of citrus may be rejected in the presence of a single moth (Moore 2012). Since the bulk of citrus fruit production in South Africa is exported, the control of T. leucotreta is critical (Citrus Growers Association, South Africa 2012). Traditionally, control has been achieved through the use of chemical insecticides; however, residue restrictions, resistance development and concerns about environmental pollution have substantially reduced the dependence on chemical pesticides in citrus. Research on T. leucotreta control has therefore focused on the use of biological organisms (e.g. parasitoids and viruses), which are used as control agents within an integrated pest management (IPM) programme in citrus. These biological control agents, however, only targeted the aboveground life stages of the pest, not the soil-dwelling life stages (late fifth instars, prepupae, pupae), which is the subject of this contribution (Moore 2012).
- Full Text:
- Date Issued: 2015
Book Review Growing the next generation of researchers: A handbook for emerging researchers and their mentors, Holness, L
- Motshoane, Puleng, Muthama, Evelyn, McKenna, Sioux
- Authors: Motshoane, Puleng , Muthama, Evelyn , McKenna, Sioux
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187205 , vital:44579 , xlink:href="https://doi.org/10.14426/cristal.v3i2.56"
- Description: South Africa urgently needs more researchers (NRF, 2008; NDP, 2011). We also need a transformation in the demographics of our researchers. One indicator of this is that currently only 14% of university professors are black African, and only 2% are black African females (DHET, 2012). The Staffing South Africa's Universities Framework includes a number of initiatives to drive the process of growing the next generation of academics. For example, the nGAP project has inserted 125 new posts into the higher education system in 2015, with more to follow. This project allows for new academics to undertake postgraduate study and develop as teachers and researchers through mentorship, a reduced teaching load and so on.
- Full Text:
- Date Issued: 2015
- Authors: Motshoane, Puleng , Muthama, Evelyn , McKenna, Sioux
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187205 , vital:44579 , xlink:href="https://doi.org/10.14426/cristal.v3i2.56"
- Description: South Africa urgently needs more researchers (NRF, 2008; NDP, 2011). We also need a transformation in the demographics of our researchers. One indicator of this is that currently only 14% of university professors are black African, and only 2% are black African females (DHET, 2012). The Staffing South Africa's Universities Framework includes a number of initiatives to drive the process of growing the next generation of academics. For example, the nGAP project has inserted 125 new posts into the higher education system in 2015, with more to follow. This project allows for new academics to undertake postgraduate study and develop as teachers and researchers through mentorship, a reduced teaching load and so on.
- Full Text:
- Date Issued: 2015
Book Review: A Renegade called Simphiwe
- Authors: Magadla, Siphokazi
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298595 , vital:57719 , xlink:href="https://doi.org/10.1177/0021909614533638"
- Description: A Renegade called Simphiwe is a “creative-intellectual portrait” of the public (and private) life of the musician Simphiwe Dana (p. 150). Gqola defines the book as “one writer’s engagement with the Simphiwe Dana of the South African public imagination [who]… troubles many categories of belonging in the South African public imagination in remarkable ways” (pp. 17, 32). The book comes at a poignant time as South Africa reflects on the success and challenges of the first 20 years of democracy. Fittingly, Gqola positions Dana within a long tradition of griots in Africa whose art always spoke truth to power.
- Full Text:
- Date Issued: 2015
- Authors: Magadla, Siphokazi
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298595 , vital:57719 , xlink:href="https://doi.org/10.1177/0021909614533638"
- Description: A Renegade called Simphiwe is a “creative-intellectual portrait” of the public (and private) life of the musician Simphiwe Dana (p. 150). Gqola defines the book as “one writer’s engagement with the Simphiwe Dana of the South African public imagination [who]… troubles many categories of belonging in the South African public imagination in remarkable ways” (pp. 17, 32). The book comes at a poignant time as South Africa reflects on the success and challenges of the first 20 years of democracy. Fittingly, Gqola positions Dana within a long tradition of griots in Africa whose art always spoke truth to power.
- Full Text:
- Date Issued: 2015
Book Review: Global Governance and the New Wars
- Authors: Magadla, Siphokazi
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298640 , vital:57723 , xlink:href="https://doi.org/10.1080/02589346.2015.1008676"
- Description: Mark Duffield's second edition of ‘Global Governance and the New Wars’ offers an important and biting critique of how different actors within the security and development discourse have adapted to the various transformations of war in the post-cold war era. In this picture drawn by Duffield, the power of states in the South continues to be eroded by an exclusionary market that is driven by the global political economy wherein state's development and security responsibilities are increasingly assumed by non-state actors (predominately constituted by Western aid agencies). Those who fall outside the bounds of the state, development and humanitarian aid agencies can be found operating in an expanding shadow economy that is also shaped by a global dynamics which make the conditions for ‘network war' possible. In this context, the lines between ‘war' and peace” are difficult to distinguish. Overall, the book paints a depressing picture on the lack of substantive changes in the livelihoods of the poor as attention has been directed to discussions about ‘new wars' or altered forms of violence that have characterized the post-cold war era. The book unforgivingly exposes the failures of the discursive changes post-cold war to reconceptualize development and security in terms that move beyond description and into substantive change especially regarding shifting the development discourse from its historic modernizing impulses.
- Full Text:
- Date Issued: 2015
- Authors: Magadla, Siphokazi
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/298640 , vital:57723 , xlink:href="https://doi.org/10.1080/02589346.2015.1008676"
- Description: Mark Duffield's second edition of ‘Global Governance and the New Wars’ offers an important and biting critique of how different actors within the security and development discourse have adapted to the various transformations of war in the post-cold war era. In this picture drawn by Duffield, the power of states in the South continues to be eroded by an exclusionary market that is driven by the global political economy wherein state's development and security responsibilities are increasingly assumed by non-state actors (predominately constituted by Western aid agencies). Those who fall outside the bounds of the state, development and humanitarian aid agencies can be found operating in an expanding shadow economy that is also shaped by a global dynamics which make the conditions for ‘network war' possible. In this context, the lines between ‘war' and peace” are difficult to distinguish. Overall, the book paints a depressing picture on the lack of substantive changes in the livelihoods of the poor as attention has been directed to discussions about ‘new wars' or altered forms of violence that have characterized the post-cold war era. The book unforgivingly exposes the failures of the discursive changes post-cold war to reconceptualize development and security in terms that move beyond description and into substantive change especially regarding shifting the development discourse from its historic modernizing impulses.
- Full Text:
- Date Issued: 2015
Can Perceptions of Environmental and Climate Change in Island Communities Assist in Adaptation Planning Locally?
- Aswani, Shankar, Vaccaro, Ismael, Abernethy, Kirsten, Albert, Simon, De Pablo, Javier Fernández-López
- Authors: Aswani, Shankar , Vaccaro, Ismael , Abernethy, Kirsten , Albert, Simon , De Pablo, Javier Fernández-López
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/421841 , vital:71889 , xlink:href="https://doi.org/10.1007/s00267-015-0572-3"
- Description: Local perceptions of environmental and climate change, as well as associated adaptations made by local populations, are fundamental for designing comprehensive and inclusive mitigation and adaptation plans both locally and nationally. In this paper, we analyze people’s perceptions of environmental and climate-related transformations in communities across the Western Solomon Islands through ethnographic and geospatial methods. Specifically, we documented people’s observed changes over the past decades across various environmental domains, and for each change, we asked respondents to identify the causes, timing, and people’s adaptive responses. We also incorporated this information into a geographical information system database to produce broad-scale base maps of local perceptions of environmental change. Results suggest that people detected changes that tended to be acute (e.g., water clarity, logging intensity, and agricultural diseases). We inferred from these results that most local observations of and adaptations to change were related to parts of environment/ecosystem that are most directly or indirectly related to harvesting strategies. On the other hand, people were less aware of slower insidious/chronic changes identified by scientific studies. For the Solomon Islands and similar contexts in the insular tropics, a broader anticipatory adaptation planning strategy to climate change should include a mix of local scientific studies and local observations of ongoing ecological changes.
- Full Text:
- Date Issued: 2015
- Authors: Aswani, Shankar , Vaccaro, Ismael , Abernethy, Kirsten , Albert, Simon , De Pablo, Javier Fernández-López
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/421841 , vital:71889 , xlink:href="https://doi.org/10.1007/s00267-015-0572-3"
- Description: Local perceptions of environmental and climate change, as well as associated adaptations made by local populations, are fundamental for designing comprehensive and inclusive mitigation and adaptation plans both locally and nationally. In this paper, we analyze people’s perceptions of environmental and climate-related transformations in communities across the Western Solomon Islands through ethnographic and geospatial methods. Specifically, we documented people’s observed changes over the past decades across various environmental domains, and for each change, we asked respondents to identify the causes, timing, and people’s adaptive responses. We also incorporated this information into a geographical information system database to produce broad-scale base maps of local perceptions of environmental change. Results suggest that people detected changes that tended to be acute (e.g., water clarity, logging intensity, and agricultural diseases). We inferred from these results that most local observations of and adaptations to change were related to parts of environment/ecosystem that are most directly or indirectly related to harvesting strategies. On the other hand, people were less aware of slower insidious/chronic changes identified by scientific studies. For the Solomon Islands and similar contexts in the insular tropics, a broader anticipatory adaptation planning strategy to climate change should include a mix of local scientific studies and local observations of ongoing ecological changes.
- Full Text:
- Date Issued: 2015
Change in Roviana Lagoon Coral Reef Ethnobiology
- Aswani, Shankar, Albert, Simon
- Authors: Aswani, Shankar , Albert, Simon
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/421863 , vital:71891 , xlink:href="https://doi.org/10.1007/978-3-319-23763-3"
- Description: Coral reefs are iconic for their beauty and biodiversity, and are of great socioeconomic and cultural importance for many coastal communities across the tropics. However, little is known about people’s local classification and their social and ecological relationship with these habitats. This chapter describes Roviana people’s changing ecological and social relationship with their coral reefs, which are increasingly being damaged by humans. First, we combined ecological and social data to describe people’s classification of local coral reefs in tandem with the productive practices conducted in these habitats. Second, we examined local perceptions and recognized effects of environmental and climatic changes on reefs over the last two decades. Finally, we measured changes in fishing activities and in the taxonomic systems (between 1995 and 2011) to evaluate if recent social and economic change has led to the erosion of marine indigenous ecological knowledge and associated practices. Studying people’s changing perceptions of their coral reefs is crucial to understand their ability to identify and adapt to environmental transformations. Simply, the way local people perceive the state of the environment is not only important in terms of changes in local epistemology but also has important implications for how resources are used and managed, and this information can be coupled with scientific one for a broader management strategy.
- Full Text:
- Date Issued: 2015
- Authors: Aswani, Shankar , Albert, Simon
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/421863 , vital:71891 , xlink:href="https://doi.org/10.1007/978-3-319-23763-3"
- Description: Coral reefs are iconic for their beauty and biodiversity, and are of great socioeconomic and cultural importance for many coastal communities across the tropics. However, little is known about people’s local classification and their social and ecological relationship with these habitats. This chapter describes Roviana people’s changing ecological and social relationship with their coral reefs, which are increasingly being damaged by humans. First, we combined ecological and social data to describe people’s classification of local coral reefs in tandem with the productive practices conducted in these habitats. Second, we examined local perceptions and recognized effects of environmental and climatic changes on reefs over the last two decades. Finally, we measured changes in fishing activities and in the taxonomic systems (between 1995 and 2011) to evaluate if recent social and economic change has led to the erosion of marine indigenous ecological knowledge and associated practices. Studying people’s changing perceptions of their coral reefs is crucial to understand their ability to identify and adapt to environmental transformations. Simply, the way local people perceive the state of the environment is not only important in terms of changes in local epistemology but also has important implications for how resources are used and managed, and this information can be coupled with scientific one for a broader management strategy.
- Full Text:
- Date Issued: 2015
Change in Roviana Lagoon Coral Reef Ethnobiology
- Aswani, Shankar, Albert, Simon
- Authors: Aswani, Shankar , Albert, Simon
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/421868 , vital:71892 , xlink:href="https://doi.org/10.1007/978-3-319-23763-3"
- Description: Coral reefs are iconic for their beauty and biodiversity, and are of great socioeconomic and cultural importance for many coastal communities across the tropics. However, little is known about people’s local classification and their social and ecological relationship with these habitats. This chapter describes Roviana people’s changing ecological and social relationship with their coral reefs, which are increasingly being damaged by humans. First, we combined ecological and social data to describe people’s classification of local coral reefs in tandem with the productive practices conducted in these habitats. Second, we examined local perceptions and recognized effects of environmental and climatic changes on reefs over the last two decades. Finally, we measured changes in fishing activities and in the taxonomic systems (between 1995 and 2011) to evaluate if recent social and economic change has led to the erosion of marine indigenous ecological knowledge and associated practices. Studying people’s changing perceptions of their coral reefs is crucial to understand their ability to identify and adapt to environmental transformations. Simply, the way local people perceive the state of the environment is not only important in terms of changes in local epistemology but also has important implications for how resources are used and managed, and this information can be coupled with scientific one for a broader management strategy.
- Full Text:
- Date Issued: 2015
- Authors: Aswani, Shankar , Albert, Simon
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/421868 , vital:71892 , xlink:href="https://doi.org/10.1007/978-3-319-23763-3"
- Description: Coral reefs are iconic for their beauty and biodiversity, and are of great socioeconomic and cultural importance for many coastal communities across the tropics. However, little is known about people’s local classification and their social and ecological relationship with these habitats. This chapter describes Roviana people’s changing ecological and social relationship with their coral reefs, which are increasingly being damaged by humans. First, we combined ecological and social data to describe people’s classification of local coral reefs in tandem with the productive practices conducted in these habitats. Second, we examined local perceptions and recognized effects of environmental and climatic changes on reefs over the last two decades. Finally, we measured changes in fishing activities and in the taxonomic systems (between 1995 and 2011) to evaluate if recent social and economic change has led to the erosion of marine indigenous ecological knowledge and associated practices. Studying people’s changing perceptions of their coral reefs is crucial to understand their ability to identify and adapt to environmental transformations. Simply, the way local people perceive the state of the environment is not only important in terms of changes in local epistemology but also has important implications for how resources are used and managed, and this information can be coupled with scientific one for a broader management strategy.
- Full Text:
- Date Issued: 2015
Characterization of porphyrin nanorods on fluorine doped tin oxide glass sheet
- George, Reama C, Falgenhauer, Jane, Geis, Clemens, Nyokong, Tebello, Schlettwein, Derck
- Authors: George, Reama C , Falgenhauer, Jane , Geis, Clemens , Nyokong, Tebello , Schlettwein, Derck
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193542 , vital:45344 , xlink:href="https://doi.org/10.1142/S1088424615500923"
- Description: Porphyrin nanorods (PNR) have been fabricated by electrostatic self-assembly of two oppositely charged porphyrin molecules. The free base meso-tetra-(4-phenylsulphonate) porphyrin (TPPS4)4) served as negatively charged counterpart for the positively charged metallo meso-tetra(4-NN-methylpyridyl) porphyrins (MTM’PyP) with either Sn, Co, Mn or In as central metal M. Films of PNR were prepared on fluorine doped tin oxide glass sheets (FTO) by using a drop-dry method. The electronic spectra revealed J-aggregation of the charged molecules for the colloid PNR as well as for the films. Transmission electron microscopy confirmed the formation of porphyrin nanorods. The laser microscope and scanning electron microscope (SEM) images of the PNR/FTO films showed the formation of three kinds of structures in the films which consist of differently branched or linear needles with their main axis grown in the direction of the solvent flow during preparation. During cyclic voltammetry either applying negative potentials from 0.0 V to -1.0 V or positive potentials from 0.0 V to ++2.2 V irreversible reduction or oxidation reactions were detected for the films. Consistently, SEM images taken following cyclic voltammetry showed the disintegration of the PNR on the films into smaller subunits. Spectroelectrochemical measurements showed the formation of porphyrin anionic radicals during oxidation by a decrease in the absorption intensities and broadening of spectra with an additional band appearing around 900 nm. A similar trend was observed when negative potentials were applied but in this case the cationic radical was produced. In both cases the decrease of the intensity of the J-aggregate confirms a loss of intermolecular coupling, again consistent with the smaller subunits observed in SEM analysis.
- Full Text:
- Date Issued: 2015
- Authors: George, Reama C , Falgenhauer, Jane , Geis, Clemens , Nyokong, Tebello , Schlettwein, Derck
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193542 , vital:45344 , xlink:href="https://doi.org/10.1142/S1088424615500923"
- Description: Porphyrin nanorods (PNR) have been fabricated by electrostatic self-assembly of two oppositely charged porphyrin molecules. The free base meso-tetra-(4-phenylsulphonate) porphyrin (TPPS4)4) served as negatively charged counterpart for the positively charged metallo meso-tetra(4-NN-methylpyridyl) porphyrins (MTM’PyP) with either Sn, Co, Mn or In as central metal M. Films of PNR were prepared on fluorine doped tin oxide glass sheets (FTO) by using a drop-dry method. The electronic spectra revealed J-aggregation of the charged molecules for the colloid PNR as well as for the films. Transmission electron microscopy confirmed the formation of porphyrin nanorods. The laser microscope and scanning electron microscope (SEM) images of the PNR/FTO films showed the formation of three kinds of structures in the films which consist of differently branched or linear needles with their main axis grown in the direction of the solvent flow during preparation. During cyclic voltammetry either applying negative potentials from 0.0 V to -1.0 V or positive potentials from 0.0 V to ++2.2 V irreversible reduction or oxidation reactions were detected for the films. Consistently, SEM images taken following cyclic voltammetry showed the disintegration of the PNR on the films into smaller subunits. Spectroelectrochemical measurements showed the formation of porphyrin anionic radicals during oxidation by a decrease in the absorption intensities and broadening of spectra with an additional band appearing around 900 nm. A similar trend was observed when negative potentials were applied but in this case the cationic radical was produced. In both cases the decrease of the intensity of the J-aggregate confirms a loss of intermolecular coupling, again consistent with the smaller subunits observed in SEM analysis.
- Full Text:
- Date Issued: 2015
Comparative electrocatalytic studies of nanocomposites of mixed and covalently linked multiwalled carbon nanotubes and 4-(4, 6-diaminopyrimidin-2-ylthio) phthalocyaninato cobalt (II)
- Nyoni, Stephen, Nyokong, Tebello
- Authors: Nyoni, Stephen , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189285 , vital:44834 , xlink:href="https://doi.org/10.1016/j.poly.2015.05.038"
- Description: Electrocatalytic behavior of 4-(4,6-diaminopyrimidin-2-ylthio) phthalocyaninato cobalt(II) (CoPyPc) when mixed or covalently mixed to multiwalled carbon nanotubes (MWCNTs) is reported. Infra-red spectroscopy was used to confirm amide linkage of the covalently linked nanocomposite. Rotating disk electrode (RDE) and cyclic (CV) voltammetry studies were used for the electrochemical characterization of the prepared phthalocyanine and MWCNT nanocomposite. The electrocatalytic effects of the nanocomposites of the cobalt phthalocyanine derivative were then investigated towards L-cysteine oxidation using both RDE and CV experiments, and the electrocatalytic performance of the covalently linked cobalt phthalocyanine-MWCNT was found to be superior over the mixed nanocomposite.
- Full Text:
- Date Issued: 2015
- Authors: Nyoni, Stephen , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189285 , vital:44834 , xlink:href="https://doi.org/10.1016/j.poly.2015.05.038"
- Description: Electrocatalytic behavior of 4-(4,6-diaminopyrimidin-2-ylthio) phthalocyaninato cobalt(II) (CoPyPc) when mixed or covalently mixed to multiwalled carbon nanotubes (MWCNTs) is reported. Infra-red spectroscopy was used to confirm amide linkage of the covalently linked nanocomposite. Rotating disk electrode (RDE) and cyclic (CV) voltammetry studies were used for the electrochemical characterization of the prepared phthalocyanine and MWCNT nanocomposite. The electrocatalytic effects of the nanocomposites of the cobalt phthalocyanine derivative were then investigated towards L-cysteine oxidation using both RDE and CV experiments, and the electrocatalytic performance of the covalently linked cobalt phthalocyanine-MWCNT was found to be superior over the mixed nanocomposite.
- Full Text:
- Date Issued: 2015
Comparisons of isotopic niche widths of some invasive and indigenous fauna in a South African river
- Hill, Jaclyn M, Jones, Roy W, Hill, Martin P, Weyl, Olaf L F
- Authors: Hill, Jaclyn M , Jones, Roy W , Hill, Martin P , Weyl, Olaf L F
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423711 , vital:72088 , xlink:href="https://doi.org/10.1111/fwb.12542"
- Description: Biological invasions threaten ecosystem integrity and bio-diversity, with numerous adverse implications for native flora and fauna. Established populations of two notorious freshwater invaders, the snail Tarebia granifera and the fish Pterygoplichthys disjunctivus, have been reported on three continents and are frequently predicted to be in di-rect competition with native species for dietary resources.
- Full Text:
- Date Issued: 2015
- Authors: Hill, Jaclyn M , Jones, Roy W , Hill, Martin P , Weyl, Olaf L F
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423711 , vital:72088 , xlink:href="https://doi.org/10.1111/fwb.12542"
- Description: Biological invasions threaten ecosystem integrity and bio-diversity, with numerous adverse implications for native flora and fauna. Established populations of two notorious freshwater invaders, the snail Tarebia granifera and the fish Pterygoplichthys disjunctivus, have been reported on three continents and are frequently predicted to be in di-rect competition with native species for dietary resources.
- Full Text:
- Date Issued: 2015
Density and regrowth of a forest restio (Ischyrolepis eleocharis) under harvest and non-harvest treatments in dune forests of Eastern Cape Province, South Africa
- Ruwanza, Sheunesu, Shackleton, Charlie M
- Authors: Ruwanza, Sheunesu , Shackleton, Charlie M
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/180828 , vital:43649 , xlink:href="https://doi.org/10.1007/s12231-015-9306-y"
- Description: Ischyrolepis eleocharis (Mast.) H.P.Linder is a perennial rush or sedge-like herb in the Restionaceae family and has been harvested for various uses from coastal forest dunes of South Africa. Around 2005, a ban on I. eleocharis harvesting in Bathurst coastal forest was instituted by provincial conservation authorities based on their unsubstantiated impression that the species population was decreasing. Here we report on the population density pre and post the harvest ban, as well as further explore the autecology of the species by assessing plant density relative to environmental factors (slope and aspect) and a controlled experiment on regrowth after harvesting at different intensities. The results show that the shoot density of both living and dead I. eleocharis increased significantly over the past eight years. This concurs with results from the harvesting experiment which showed that I. eleocharis recovered rapidly within a year of harvesting. Both aspect and slope play a key role in the distribution of I. eleocharis, with most plants found in open patches in the forest located on the summit and upper slopes of dunes facing the landward side. These results indicate that I. eleocharis is highly abundant and resilient to harvesting.
- Full Text:
- Date Issued: 2015
- Authors: Ruwanza, Sheunesu , Shackleton, Charlie M
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/180828 , vital:43649 , xlink:href="https://doi.org/10.1007/s12231-015-9306-y"
- Description: Ischyrolepis eleocharis (Mast.) H.P.Linder is a perennial rush or sedge-like herb in the Restionaceae family and has been harvested for various uses from coastal forest dunes of South Africa. Around 2005, a ban on I. eleocharis harvesting in Bathurst coastal forest was instituted by provincial conservation authorities based on their unsubstantiated impression that the species population was decreasing. Here we report on the population density pre and post the harvest ban, as well as further explore the autecology of the species by assessing plant density relative to environmental factors (slope and aspect) and a controlled experiment on regrowth after harvesting at different intensities. The results show that the shoot density of both living and dead I. eleocharis increased significantly over the past eight years. This concurs with results from the harvesting experiment which showed that I. eleocharis recovered rapidly within a year of harvesting. Both aspect and slope play a key role in the distribution of I. eleocharis, with most plants found in open patches in the forest located on the summit and upper slopes of dunes facing the landward side. These results indicate that I. eleocharis is highly abundant and resilient to harvesting.
- Full Text:
- Date Issued: 2015
DNA-based identification of Lepidoptera associated with citrus in South Africa
- Marsberg, Tamryn, Hill, Martin P, Moore, Sean D, Timm, A E
- Authors: Marsberg, Tamryn , Hill, Martin P , Moore, Sean D , Timm, A E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/405782 , vital:70205 , xlink:href="https://hdl.handle.net/10520/EJC167513"
- Description: A number of insects, primarily Lepidoptera, cause damage to citrus in South Africa. A major limitation to the management and control of these pests is their correct identification. The aim of this study was to develop a database of gene sequences to aid in the identification of these Lepidoptera. Multiple specimens of 12 species were sequenced for the ~650 bp of the cytochrome oxidase I gene. These sequence data were supplemented and validated using sequences available in public databases. Results showed that each species could be unambiguously identified, and neighbour-joining analysis based on K2P distances formed highly supported, distinct clusters for each species, i.e. the maximum intraspecific genetic distance was less than that of the minimum interspecific genetic distances. Thus, this data set provides a molecular means to successfully identify the most important Lepidoptera associated with citrus in South Africa.
- Full Text:
- Date Issued: 2015
- Authors: Marsberg, Tamryn , Hill, Martin P , Moore, Sean D , Timm, A E
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/405782 , vital:70205 , xlink:href="https://hdl.handle.net/10520/EJC167513"
- Description: A number of insects, primarily Lepidoptera, cause damage to citrus in South Africa. A major limitation to the management and control of these pests is their correct identification. The aim of this study was to develop a database of gene sequences to aid in the identification of these Lepidoptera. Multiple specimens of 12 species were sequenced for the ~650 bp of the cytochrome oxidase I gene. These sequence data were supplemented and validated using sequences available in public databases. Results showed that each species could be unambiguously identified, and neighbour-joining analysis based on K2P distances formed highly supported, distinct clusters for each species, i.e. the maximum intraspecific genetic distance was less than that of the minimum interspecific genetic distances. Thus, this data set provides a molecular means to successfully identify the most important Lepidoptera associated with citrus in South Africa.
- Full Text:
- Date Issued: 2015
Editorial. Perspectives on transformations in learning and education
- Authors: Lotz-Sisitka, Heila
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/387277 , vital:68221 , xlink:href="https://www.ajol.info/index.php/sajee/article/view/137656"
- Description: Perspectives on transformations in learning and education
- Full Text:
- Date Issued: 2015
- Authors: Lotz-Sisitka, Heila
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/387277 , vital:68221 , xlink:href="https://www.ajol.info/index.php/sajee/article/view/137656"
- Description: Perspectives on transformations in learning and education
- Full Text:
- Date Issued: 2015
Effects of differently shaped silver nanoparticles on the photophysics of pyridylsulfanyl-substituted phthalocyanines
- D'Souza, Sarah, Mashazi, Philani N, Britton, Jonathan, Nyokong, Tebello
- Authors: D'Souza, Sarah , Mashazi, Philani N , Britton, Jonathan , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193564 , vital:45348 , xlink:href="https://doi.org/10.1016/j.poly.2015.06.038"
- Description: This paper reports on the photophysical behavior of (2-pyridylsulfanyl)phthalocyaninato zinc(II) and 2,9(10),16(17),23(24)-tetra-(2-pyridylsulfanyl)phthalocyaninato zinc(II) in the presence of differently shaped silver nanoparticles (nanospheres, nanotriangles and nanoflowers). The presence of shaped nanoparticles increased both triplet quantum yields and lifetimes of the tetra-substituted mercaptopyridine zinc phthalocyanine in DMSO. It is apparent from this work that the shape of the silver nanoparticle used is of little consequence in influencing photophysical behavior of the phthalocyanines.
- Full Text:
- Date Issued: 2015
- Authors: D'Souza, Sarah , Mashazi, Philani N , Britton, Jonathan , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193564 , vital:45348 , xlink:href="https://doi.org/10.1016/j.poly.2015.06.038"
- Description: This paper reports on the photophysical behavior of (2-pyridylsulfanyl)phthalocyaninato zinc(II) and 2,9(10),16(17),23(24)-tetra-(2-pyridylsulfanyl)phthalocyaninato zinc(II) in the presence of differently shaped silver nanoparticles (nanospheres, nanotriangles and nanoflowers). The presence of shaped nanoparticles increased both triplet quantum yields and lifetimes of the tetra-substituted mercaptopyridine zinc phthalocyanine in DMSO. It is apparent from this work that the shape of the silver nanoparticle used is of little consequence in influencing photophysical behavior of the phthalocyanines.
- Full Text:
- Date Issued: 2015
Effects of ZnO nanohexagons and nanorods on the fluorescence behavior of metallophthalocyanines
- D'Souza, Sarah, Moeno, Sharon, Nyokong, Tebello
- Authors: D'Souza, Sarah , Moeno, Sharon , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189741 , vital:44927 , xlink:href="https://doi.org/10.1016/j.poly.2014.09.012"
- Description: This paper looks at the fluorescence behavior of zinc phthalocyanines: 2,(3),9(10),16(17),23(24)-tetrakis- (mercaptoacetic acid phthalocyaninato) zinc(II) (ZnTMAAPc), 2,(3),9(10),16(17),23(24)-tetrakis-(mercaptopropanoic acid phthalocyaninato) zinc(II) (ZnTMPAPc) and (OH)AlPcSmix (the latter contains a mixture of the di-, tri- and tetra-sulfonated derivatives with an average of three sulfonated groups per molecule) in the presence of ZnO nanoparticles. Fluorescence lifetimes of Pc complexes generally decreased in the presence of ZnO nanoparticles, with generally longer lifetimes for ZnO nanohexagons compared to ZnO nanorods.
- Full Text:
- Date Issued: 2015
- Authors: D'Souza, Sarah , Moeno, Sharon , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189741 , vital:44927 , xlink:href="https://doi.org/10.1016/j.poly.2014.09.012"
- Description: This paper looks at the fluorescence behavior of zinc phthalocyanines: 2,(3),9(10),16(17),23(24)-tetrakis- (mercaptoacetic acid phthalocyaninato) zinc(II) (ZnTMAAPc), 2,(3),9(10),16(17),23(24)-tetrakis-(mercaptopropanoic acid phthalocyaninato) zinc(II) (ZnTMPAPc) and (OH)AlPcSmix (the latter contains a mixture of the di-, tri- and tetra-sulfonated derivatives with an average of three sulfonated groups per molecule) in the presence of ZnO nanoparticles. Fluorescence lifetimes of Pc complexes generally decreased in the presence of ZnO nanoparticles, with generally longer lifetimes for ZnO nanohexagons compared to ZnO nanorods.
- Full Text:
- Date Issued: 2015
Electrocatalytic activity of bimetallic Au–Pd nanoparticles in the presence of cobalt tetraaminophthalocyanine
- Maringa, Audacity, Mashazi, Philani N, Nyokong, Tebello
- Authors: Maringa, Audacity , Mashazi, Philani N , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189719 , vital:44925 , xlink:href="https://doi.org/10.1016/j.jcis.2014.10.056"
- Description: Au and Pd nanoparticles were individually or together electrodeposited on top of polymerized cobalt tetraaminophthalocyanine (poly-CoTAPc). When Pd and Au nanoparticles are co-deposited together, the electrode is denoted as Au–Pd (co-deposited)/poly-CoTAPc-GCE. X-ray photoelectron spectroscopy (XPS) was used to show the successful deposition of AuNPs, PdNPs and Au–Pd (co-deposited). The scanning electrochemical microscopy showed that Au–Pd (co-deposited)/poly-CoTAPc-GCE (with current range of 9.5–13.5 lA) was more conducting than Au–Pd (co-deposited)-GCE (with current range of 8–12 lA). Electrochemical impedance spectroscopy (EIS) showed that there was less resistance to charge transfer for Au–Pd (co-deposited)/poly-CoTAPc-GCE compared to the rest of the electrodes. Au–Pd (co-deposited)/poly-CoTAPc-GCE showed the best activity for the electrooxidation of hydrazine in terms of limit of detection (0.5 lM), hence shows promise as an electrocatalyst for electrooxidation of hydrazine.
- Full Text:
- Date Issued: 2015
- Authors: Maringa, Audacity , Mashazi, Philani N , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189719 , vital:44925 , xlink:href="https://doi.org/10.1016/j.jcis.2014.10.056"
- Description: Au and Pd nanoparticles were individually or together electrodeposited on top of polymerized cobalt tetraaminophthalocyanine (poly-CoTAPc). When Pd and Au nanoparticles are co-deposited together, the electrode is denoted as Au–Pd (co-deposited)/poly-CoTAPc-GCE. X-ray photoelectron spectroscopy (XPS) was used to show the successful deposition of AuNPs, PdNPs and Au–Pd (co-deposited). The scanning electrochemical microscopy showed that Au–Pd (co-deposited)/poly-CoTAPc-GCE (with current range of 9.5–13.5 lA) was more conducting than Au–Pd (co-deposited)-GCE (with current range of 8–12 lA). Electrochemical impedance spectroscopy (EIS) showed that there was less resistance to charge transfer for Au–Pd (co-deposited)/poly-CoTAPc-GCE compared to the rest of the electrodes. Au–Pd (co-deposited)/poly-CoTAPc-GCE showed the best activity for the electrooxidation of hydrazine in terms of limit of detection (0.5 lM), hence shows promise as an electrocatalyst for electrooxidation of hydrazine.
- Full Text:
- Date Issued: 2015
Electrocatalytic studies of covalently immobilized metal tetra-amino phthalocyanines onto derivatized screen-printed gold electrodes
- Mashazi, Philani N, Nyokong, Tebello
- Authors: Mashazi, Philani N , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/249059 , vital:51774 , xlink:href="https://doi.org/10.1007/s00604-010-0438-6"
- Description: Metal tetra-amino phthalocyanine complexes (MTAPc; where M is Co or Mn) were immobilized on screen-printed gold electrodes pre-modified with monolayers of benzylamino groups. The functionalized electrodes were then activated using benzene-1,4-dicarbaldehyde as a linker before MTAPc complexes were immobilized. The surface coverages for the modified electrodes confirmed the perpendicular orientation of the MTAPcs. The apparent electron transfer constant (kapp) for the electrodes is 2.2 × 10−5 cm.s−1 for both CoTAPc and MnTAPc modified electrodes as calculated with data from impedance measurements. The kapp values for the bare and benzylamino modified electrodes were found to be 1.2 × 10−4 cm.s−1 and 4.9 × 10−6 cm.s−1, respectively. The electrocatalysis of the modified electrodes towards detection of H2O2 gave significant peak current densities and electrocatalytic potentials at −0.28 V and −0.31 V for the MnTAPc and CoTAPc modified electrodes, respectively.
- Full Text:
- Date Issued: 2015
- Authors: Mashazi, Philani N , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/249059 , vital:51774 , xlink:href="https://doi.org/10.1007/s00604-010-0438-6"
- Description: Metal tetra-amino phthalocyanine complexes (MTAPc; where M is Co or Mn) were immobilized on screen-printed gold electrodes pre-modified with monolayers of benzylamino groups. The functionalized electrodes were then activated using benzene-1,4-dicarbaldehyde as a linker before MTAPc complexes were immobilized. The surface coverages for the modified electrodes confirmed the perpendicular orientation of the MTAPcs. The apparent electron transfer constant (kapp) for the electrodes is 2.2 × 10−5 cm.s−1 for both CoTAPc and MnTAPc modified electrodes as calculated with data from impedance measurements. The kapp values for the bare and benzylamino modified electrodes were found to be 1.2 × 10−4 cm.s−1 and 4.9 × 10−6 cm.s−1, respectively. The electrocatalysis of the modified electrodes towards detection of H2O2 gave significant peak current densities and electrocatalytic potentials at −0.28 V and −0.31 V for the MnTAPc and CoTAPc modified electrodes, respectively.
- Full Text:
- Date Issued: 2015
Electrode modification using alkynyl substituted Fe (II) phthalocyanine via electrografting and click chemistry for electrocatalysis
- Nxele, Siphesihle R, Mashazi, Philani N, Nyokong, Tebello
- Authors: Nxele, Siphesihle R , Mashazi, Philani N , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189296 , vital:44835 , xlink:href="https://doi.org/10.1002/elan.201500212"
- Description: In this work, tetrakis(5-hexyn-oxy)Fe(II) phthalocyanine was synthesised in order to perform a click reaction between the terminal alkyne groups and an azide group on a glassy carbon electrode (GCE) surface. An azide group was formed on the electrode surface following electrografting using 4-azidobenzene diazonium tetrafluoroborate by electrochemical reduction. The Cu(I) catalyzed alkyne-azide Huisgen cycloaddition reaction was then employed in order to react the terminal alkyne groups on the phthalocyanine with the azide groups on the GCE surface. The modified electrode was employed to catalyse the oxidation of hydrazine. The electrode showed good electrocatalytic ability towards the detection of hydrazine with a sensitivity of 15.38 µA mM−1 and a limit of detection of 1.09 µM.
- Full Text:
- Date Issued: 2015
- Authors: Nxele, Siphesihle R , Mashazi, Philani N , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189296 , vital:44835 , xlink:href="https://doi.org/10.1002/elan.201500212"
- Description: In this work, tetrakis(5-hexyn-oxy)Fe(II) phthalocyanine was synthesised in order to perform a click reaction between the terminal alkyne groups and an azide group on a glassy carbon electrode (GCE) surface. An azide group was formed on the electrode surface following electrografting using 4-azidobenzene diazonium tetrafluoroborate by electrochemical reduction. The Cu(I) catalyzed alkyne-azide Huisgen cycloaddition reaction was then employed in order to react the terminal alkyne groups on the phthalocyanine with the azide groups on the GCE surface. The modified electrode was employed to catalyse the oxidation of hydrazine. The electrode showed good electrocatalytic ability towards the detection of hydrazine with a sensitivity of 15.38 µA mM−1 and a limit of detection of 1.09 µM.
- Full Text:
- Date Issued: 2015
Enhanced optical limiting behaviour of indium phthalocyanine derivatives when in solution or embedded in poly (acrylic acid) or poly (methyl methacrylate) polymers
- Sanusi, Kayode, Nyokong, Tebello
- Authors: Sanusi, Kayode , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189445 , vital:44847 , xlink:href="https://doi.org/10.1016/j.jphotochem.2015.02.003"
- Description: The optical limiting performance of indium phthalocyanine-based polymer thin-films with large nonlinear absorption coefficients (βeff) and low limiting threshold intensity (Ilim) are described. The absorption cross-sections and the population dynamics of the excited states are also reported. The excited state absorption cross-sections (σexc) are shown to depend on the transition moment between the T1 and T2 states. βeff values have been shown to be related to the population density of the molecules in the T1 state. The improved optical limiting performance recorded for the investigated phthalocyanine complexes in the presence of polymer matrices has been attributed to the aggregation effects of the complexes in the polymer thin-films. The optical properties of the indium phthalocyanine moieties were found to possess robust sensitivity to a change of the polymer materials.
- Full Text:
- Date Issued: 2015
- Authors: Sanusi, Kayode , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189445 , vital:44847 , xlink:href="https://doi.org/10.1016/j.jphotochem.2015.02.003"
- Description: The optical limiting performance of indium phthalocyanine-based polymer thin-films with large nonlinear absorption coefficients (βeff) and low limiting threshold intensity (Ilim) are described. The absorption cross-sections and the population dynamics of the excited states are also reported. The excited state absorption cross-sections (σexc) are shown to depend on the transition moment between the T1 and T2 states. βeff values have been shown to be related to the population density of the molecules in the T1 state. The improved optical limiting performance recorded for the investigated phthalocyanine complexes in the presence of polymer matrices has been attributed to the aggregation effects of the complexes in the polymer thin-films. The optical properties of the indium phthalocyanine moieties were found to possess robust sensitivity to a change of the polymer materials.
- Full Text:
- Date Issued: 2015