Women's empowerment in the post-1994 Rwanda: the case study of Mayaga Region
- Authors: Hategekimana, Celestin
- Date: 2011
- Subjects: Women in development -- Rwanda , Women -- Rwanda -- Social conditions , Women -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9068 , http://hdl.handle.net/10948/1314 , Women in development -- Rwanda , Women -- Rwanda -- Social conditions , Women -- Economic conditions
- Description: This research looks at the process of women’s empowerment in post- 1994 Rwanda, with special focus on twelve cooperatives working in Mayaga region and the way these cooperatives empower women, their households and the community at large. Traditional Rwandan society has been always bound by patriarchy which has not valued the reproductive roles of women as economically productive in their households and the society as a whole. On the one hand, this understanding was reversed in the post-1994 Rwanda by the commitment of the government to gender equality at the highest level of political leadership through progressive policies and legislation. On the other hand, in Mayaga region, cooperatives brought about socio-economic development and changed relationships of gender and power in a patriarchal post-conflict society. The findings from cooperatives in Mayaga region show that to prevent women from reaching their full potential is economic folly. If women are empowered, they can generate important development outcomes such as improved health, education, income levels and conflict resolution. The findings further indicate how women’s empowerment is determined by the livelihood strategies women adopt themselves to respond to their vulnerability, and by the ways in which they express their agency in making a living in a sustainable way, with the available community assets that they have access to (financial, social, human, natural and physical). This research highlights that the accessibility of the community assets used by women in Mayaga region and in Rwanda as a whole is also determined by policies, institutions and processes that are able to influence their livelihoods positively.
- Full Text:
- Date Issued: 2011
- Authors: Hategekimana, Celestin
- Date: 2011
- Subjects: Women in development -- Rwanda , Women -- Rwanda -- Social conditions , Women -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9068 , http://hdl.handle.net/10948/1314 , Women in development -- Rwanda , Women -- Rwanda -- Social conditions , Women -- Economic conditions
- Description: This research looks at the process of women’s empowerment in post- 1994 Rwanda, with special focus on twelve cooperatives working in Mayaga region and the way these cooperatives empower women, their households and the community at large. Traditional Rwandan society has been always bound by patriarchy which has not valued the reproductive roles of women as economically productive in their households and the society as a whole. On the one hand, this understanding was reversed in the post-1994 Rwanda by the commitment of the government to gender equality at the highest level of political leadership through progressive policies and legislation. On the other hand, in Mayaga region, cooperatives brought about socio-economic development and changed relationships of gender and power in a patriarchal post-conflict society. The findings from cooperatives in Mayaga region show that to prevent women from reaching their full potential is economic folly. If women are empowered, they can generate important development outcomes such as improved health, education, income levels and conflict resolution. The findings further indicate how women’s empowerment is determined by the livelihood strategies women adopt themselves to respond to their vulnerability, and by the ways in which they express their agency in making a living in a sustainable way, with the available community assets that they have access to (financial, social, human, natural and physical). This research highlights that the accessibility of the community assets used by women in Mayaga region and in Rwanda as a whole is also determined by policies, institutions and processes that are able to influence their livelihoods positively.
- Full Text:
- Date Issued: 2011
Using social marketing to bridge the gap between systematic conservation planning and implementation at the local government level
- Authors: Wilhelm-Rechmann, Angelika
- Date: 2011
- Subjects: Nature conservation -- South Africa -- Eastern Cape , Land use -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10603 , http://hdl.handle.net/10948/1550 , Nature conservation -- South Africa -- Eastern Cape , Land use -- South Africa -- Eastern Cape
- Description: The study presented here describes an attempt to bridge the gap between systematic conservation assessment and decision-making for land-use planning in the Eastern Cape province, South Africa. The aim was to investigate how to effectively convince officials concerned with land use planning processes in the local municipal sphere to include conservation priorities meaningfully in their processes. The approach used to reach this aim was social marketing, the use of marketing technologies and concepts to effect behavior changes to further societal good. So far social marketing is not commonly used in the conservation domain; I therefore aimed also at proving the usefulness of this approach for conservation. Following the introduction which provides background to the project and a more detailed summary, Chapter 2 provides a detailed and comprehensive review of the considerations and concepts regarding the use of social marketing in a context geared at protecting nature. The research on the primary target group for this study, officials concerned with land use planning processes in the local municipal sphere is described in Chapter 3. The main outcomes were that land use planners perceive few needs with regards to implementing the incorporation of biodiversity conservation issues in the land use planning process, and that the deficiencies in the land use planning process per se, as well as the lack of recognition in the political sphere (the domain of elected councilors), represent the core barriers to adopting the conservation priorities. I conclude that to effect behavior change towards adoption of conservation priorities the land use planning processes need to be supported and the political sphere need to be included in the behavior change process. 6 Chapter 4 therefore focuses on the new target group that emerged as essential in the previous chapter, locally elected councilors. I found that councilors do actually consider land use planning procedures as being important, but also as being dysfunctional. Councilors do value their natural environment for themselves as well as for its tourism value, but most councilors had little understanding of what the term “biodiversity” means and did not connect the term “sustainability” with the natural environment. It became also evident, that councilors do not see conservation in a predominantly positive manner. Chapter 5 therefore yields insight on councilor’s perception that environmental protection and development are mutually exclusive, and the negative frames attached to the conservation endeavor as being socially unjust, disrespectful and utopian. In Chapter 6 I investigated the usefulness of a tractable and well established measure of environmental attitudes or beliefs. I assessed my target audience’s responses to the New Ecological Paradigm scale and the Inclusion of Nature in Self scale. I conclude in Chapter 7 with an account of the difficulties I encountered during the project, an assessment of my project from a social marketing perspective, components of my project that did not yield the results expected, and a proposal for future research.
- Full Text:
- Date Issued: 2011
- Authors: Wilhelm-Rechmann, Angelika
- Date: 2011
- Subjects: Nature conservation -- South Africa -- Eastern Cape , Land use -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10603 , http://hdl.handle.net/10948/1550 , Nature conservation -- South Africa -- Eastern Cape , Land use -- South Africa -- Eastern Cape
- Description: The study presented here describes an attempt to bridge the gap between systematic conservation assessment and decision-making for land-use planning in the Eastern Cape province, South Africa. The aim was to investigate how to effectively convince officials concerned with land use planning processes in the local municipal sphere to include conservation priorities meaningfully in their processes. The approach used to reach this aim was social marketing, the use of marketing technologies and concepts to effect behavior changes to further societal good. So far social marketing is not commonly used in the conservation domain; I therefore aimed also at proving the usefulness of this approach for conservation. Following the introduction which provides background to the project and a more detailed summary, Chapter 2 provides a detailed and comprehensive review of the considerations and concepts regarding the use of social marketing in a context geared at protecting nature. The research on the primary target group for this study, officials concerned with land use planning processes in the local municipal sphere is described in Chapter 3. The main outcomes were that land use planners perceive few needs with regards to implementing the incorporation of biodiversity conservation issues in the land use planning process, and that the deficiencies in the land use planning process per se, as well as the lack of recognition in the political sphere (the domain of elected councilors), represent the core barriers to adopting the conservation priorities. I conclude that to effect behavior change towards adoption of conservation priorities the land use planning processes need to be supported and the political sphere need to be included in the behavior change process. 6 Chapter 4 therefore focuses on the new target group that emerged as essential in the previous chapter, locally elected councilors. I found that councilors do actually consider land use planning procedures as being important, but also as being dysfunctional. Councilors do value their natural environment for themselves as well as for its tourism value, but most councilors had little understanding of what the term “biodiversity” means and did not connect the term “sustainability” with the natural environment. It became also evident, that councilors do not see conservation in a predominantly positive manner. Chapter 5 therefore yields insight on councilor’s perception that environmental protection and development are mutually exclusive, and the negative frames attached to the conservation endeavor as being socially unjust, disrespectful and utopian. In Chapter 6 I investigated the usefulness of a tractable and well established measure of environmental attitudes or beliefs. I assessed my target audience’s responses to the New Ecological Paradigm scale and the Inclusion of Nature in Self scale. I conclude in Chapter 7 with an account of the difficulties I encountered during the project, an assessment of my project from a social marketing perspective, components of my project that did not yield the results expected, and a proposal for future research.
- Full Text:
- Date Issued: 2011
Trophodynamics of the benthic and hyperbenthic communities inhabiting the Sub-Antarctic Prince Edward Islands : stable isotope and fatty acid signatures
- Authors: Allan, Elizabeth Louise
- Date: 2011
- Subjects: Benthos -- Prince Edward Islands Benthos -- Feeding and feeds -- Prince Edward Islands Benthos -- Nutrition -- Prince Edward Islands Prince Edward Islands
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5806 , http://hdl.handle.net/10962/d1006350
- Description: The aim of this study was to investigate spatial changes in the trophic and energy pathways of the benthic community in the shallow shelf waters of the sub-Antarctic Prince Edward Islands (PEI). A combination of stable isotope and fatty acid analyses were used to provide a time-integrated view of the assimilated feeding history of selected components of the PEI benthic community. This study forms part of the larger project entitled “Variability in the Southern Ocean ecosystems” and is a contribution to the South African National Antarctic Programme (SANAP). During austral autumn 2009, benthic specimens were collected from 10 stations (from depths of 70 to 295 m) in different regions around the PEI: inter-island shelf (upstream, between and downstream of the islands) and nearshore. Historical data were combined with new data collected during 2009 to assess the long-term trends in the feeding ecology of the benthos in the region of the islands. The stable isotope and fatty acid signatures of the benthic suspension- and deposit-feeding organisms generated during this study suggested that these two communities incorporated both phytoplankton and kelp in their diets. Stable isotope, and to a lesser extent fatty acid signatures, indicated that kelp contributed more to the diets of those organisms in close proximity to the kelp beds (nearshore stations) than those from the inter-island region. Overall, however, pelagic phytoplankton was the dominant food source in the diets of all organisms, even for those living near the kelp beds. Notable exceptions were the sponges and bryozoans, in which kelp and phytoplankton contributed similar proportions to their diets, most likely resulting from a size restricted feeding mode. There were, therefore, no distinct spatial differences in the importance of the various food sources. However, fatty acid compositional data indicated increased food quality between and within the lee of the islands compared to upstream. The organisms collected upstream of the PEI had substantially lower quantities of total fatty acids (TFAs) than organisms of the same species collected from nearshore, open shelf or downstream stations. The increased food quality between and within the lee of the islands was likely a result of the “island mass effect”, which reflects increased phytoplankton concentrations at the PEI. The fatty acid profiles of hyperbenthic shrimp Nauticaris marionis, a key species in the PEI ecosystem, revealed no distinct ontogenetic or spatial patterns. This result is in contrast to the stable isotope analyses, which detected both spatial and ontogenetic differences in the diet of the shrimp. Nearshore shrimp were more ₁₃C-enriched than those from the inter-island region, suggesting increased kelp entering the food web within these regions. In addition, the shrimps demonstrated enrichment in δ₁₃C and δ₁₅N signatures with an increase in size, resulting in a relatively distinct separation of size classes, thus reflecting niche separation through their diets. The fatty acid profiles revealed that the shrimp all contained large proportions of polyunsaturated fatty acids (PUFAs) and essential fatty acids (EFAs), indicating that the quality of food consumed was similar among size class and region despite the niche separation and variation in carbon sources utilised. In addition, diatom and dinoflagellate fatty acids (20:5ω3 and 22:6ω3, respectively) occurred in the highest proportions in N. marionis, highlighting the importance of phytoplankton (indirectly) in their diet. These results represent strong evidence that phytoplankton is an importance food source in the PEI ecosystem. The temporal investigation of isotope signatures in the benthos at the PEI indicated that N. marionis demonstrated an overall depletion in δ₁₃C signatures over the period of 1984 to 2009 (nearshore: -2.55 ‰, inter-island: -2.32 ‰). Overall, the benthic community showed similar depletions in δ13C signatures (from -1.96 to -4.70 ‰), suggesting that shifts have occurred in the carbon signatures at the base of the food web. The depletion in δ₁₃C signatures of the benthos at the PEI most likely reflects increased contributions of smaller slow growing phytoplankton cells (more depleted signatures than large fast growing cells) in the diets of these organisms over time. These diet shifts, in turn, suggest a decrease in productivity has occurred at the islands, likely due to a decreased frequency and intensity of the “island mass effect”. Decreased phytoplankton productivity at the PEI likely results from the southward shift in the average position of the sub-Antarctic Front (SAF), most likely in response to climate change, which in turn promotes flow-through conditions rather than retention at the PEI.
- Full Text:
- Date Issued: 2011
- Authors: Allan, Elizabeth Louise
- Date: 2011
- Subjects: Benthos -- Prince Edward Islands Benthos -- Feeding and feeds -- Prince Edward Islands Benthos -- Nutrition -- Prince Edward Islands Prince Edward Islands
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5806 , http://hdl.handle.net/10962/d1006350
- Description: The aim of this study was to investigate spatial changes in the trophic and energy pathways of the benthic community in the shallow shelf waters of the sub-Antarctic Prince Edward Islands (PEI). A combination of stable isotope and fatty acid analyses were used to provide a time-integrated view of the assimilated feeding history of selected components of the PEI benthic community. This study forms part of the larger project entitled “Variability in the Southern Ocean ecosystems” and is a contribution to the South African National Antarctic Programme (SANAP). During austral autumn 2009, benthic specimens were collected from 10 stations (from depths of 70 to 295 m) in different regions around the PEI: inter-island shelf (upstream, between and downstream of the islands) and nearshore. Historical data were combined with new data collected during 2009 to assess the long-term trends in the feeding ecology of the benthos in the region of the islands. The stable isotope and fatty acid signatures of the benthic suspension- and deposit-feeding organisms generated during this study suggested that these two communities incorporated both phytoplankton and kelp in their diets. Stable isotope, and to a lesser extent fatty acid signatures, indicated that kelp contributed more to the diets of those organisms in close proximity to the kelp beds (nearshore stations) than those from the inter-island region. Overall, however, pelagic phytoplankton was the dominant food source in the diets of all organisms, even for those living near the kelp beds. Notable exceptions were the sponges and bryozoans, in which kelp and phytoplankton contributed similar proportions to their diets, most likely resulting from a size restricted feeding mode. There were, therefore, no distinct spatial differences in the importance of the various food sources. However, fatty acid compositional data indicated increased food quality between and within the lee of the islands compared to upstream. The organisms collected upstream of the PEI had substantially lower quantities of total fatty acids (TFAs) than organisms of the same species collected from nearshore, open shelf or downstream stations. The increased food quality between and within the lee of the islands was likely a result of the “island mass effect”, which reflects increased phytoplankton concentrations at the PEI. The fatty acid profiles of hyperbenthic shrimp Nauticaris marionis, a key species in the PEI ecosystem, revealed no distinct ontogenetic or spatial patterns. This result is in contrast to the stable isotope analyses, which detected both spatial and ontogenetic differences in the diet of the shrimp. Nearshore shrimp were more ₁₃C-enriched than those from the inter-island region, suggesting increased kelp entering the food web within these regions. In addition, the shrimps demonstrated enrichment in δ₁₃C and δ₁₅N signatures with an increase in size, resulting in a relatively distinct separation of size classes, thus reflecting niche separation through their diets. The fatty acid profiles revealed that the shrimp all contained large proportions of polyunsaturated fatty acids (PUFAs) and essential fatty acids (EFAs), indicating that the quality of food consumed was similar among size class and region despite the niche separation and variation in carbon sources utilised. In addition, diatom and dinoflagellate fatty acids (20:5ω3 and 22:6ω3, respectively) occurred in the highest proportions in N. marionis, highlighting the importance of phytoplankton (indirectly) in their diet. These results represent strong evidence that phytoplankton is an importance food source in the PEI ecosystem. The temporal investigation of isotope signatures in the benthos at the PEI indicated that N. marionis demonstrated an overall depletion in δ₁₃C signatures over the period of 1984 to 2009 (nearshore: -2.55 ‰, inter-island: -2.32 ‰). Overall, the benthic community showed similar depletions in δ13C signatures (from -1.96 to -4.70 ‰), suggesting that shifts have occurred in the carbon signatures at the base of the food web. The depletion in δ₁₃C signatures of the benthos at the PEI most likely reflects increased contributions of smaller slow growing phytoplankton cells (more depleted signatures than large fast growing cells) in the diets of these organisms over time. These diet shifts, in turn, suggest a decrease in productivity has occurred at the islands, likely due to a decreased frequency and intensity of the “island mass effect”. Decreased phytoplankton productivity at the PEI likely results from the southward shift in the average position of the sub-Antarctic Front (SAF), most likely in response to climate change, which in turn promotes flow-through conditions rather than retention at the PEI.
- Full Text:
- Date Issued: 2011
Trials and triumphs in public office: the life and work of E J N Mabuza
- Authors: Sarimana, Ashley
- Date: 2011
- Subjects: Mabuza, E J (Enos John), 1939- Public officers -- South Africa South Africa -- Politics and government -- 20th century South Africa -- History -- 20th century South Africa -- Social conditions -- 20th century Homelands (South Africa) -- Politics and government -- 20th century Homelands (South Africa) -- History -- 20th century Homelands (South Africa) -- Social conditions -- 20th century Apartheid -- South Africa South Africa -- Race relations Actor-network theory
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3305 , http://hdl.handle.net/10962/d1003093
- Description: Enos John Nganani Mabuza's life and work is used as a case study to highlight the conceptual and methodological theories and challenges in academic biographical writing regarding history, memory and legacies. This thesis answers the question: Who was Mabuza and what is his place or relevance in South Africa's history? We over-simplify and stereotype people because it is convenient. We judge others but there is no saint without a past or a sinner without a future. Mabuza reconciled with the main liberation movements and moved from a maligned position on the political fringes to a respectable one within the emerging black economic elite. History, like reference systems and values is not calcified or static. It is prone to interpretation, adaptation, modification, invention, manipulation, decomposition and re-composition. Bourdieu's habitus-field analysis, theory or logic of practice, notions of capital (symbolic, political, social, cultural and economic) is used together with Latour's actor-network theory as the basis of analysis of the social contracts and trust bonds that Mabuza was able to create and which enabled him to navigate South Africa's socio-political and economic milieus during apartheid and the transition in the early 1990s. As people or actors, we believe in the mantra, nothing ventured, nothing gained. We exercise agency and take risks every day. We make choices and those choices have consequences. Mabuza's choices in the fields of education, politics and business had implications for how he is perceived or has been written into history. His choices put notions of identity, citizenship, power, legitimacy, ambition, elite accommodation, class, personal and professional networks, popular struggles, agency and structure under the spotlight. Mabuza's involvement in Bantustan politics, for instance, is contextualised in terms of a historical overview of the unpopular role played by traditional authorities in South Africa before and during colonialism and apartheid. His later foray into the world of business, however, was facilitated in part, by the personal and professional contacts that he made whilst he was in politics and the opportunities which opened up during the country's political transition. Mabuza adapted to changing circumstances and demonstrated a level of versatility which other Bantustan functionaries did not or could not exercise.
- Full Text:
- Date Issued: 2011
- Authors: Sarimana, Ashley
- Date: 2011
- Subjects: Mabuza, E J (Enos John), 1939- Public officers -- South Africa South Africa -- Politics and government -- 20th century South Africa -- History -- 20th century South Africa -- Social conditions -- 20th century Homelands (South Africa) -- Politics and government -- 20th century Homelands (South Africa) -- History -- 20th century Homelands (South Africa) -- Social conditions -- 20th century Apartheid -- South Africa South Africa -- Race relations Actor-network theory
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3305 , http://hdl.handle.net/10962/d1003093
- Description: Enos John Nganani Mabuza's life and work is used as a case study to highlight the conceptual and methodological theories and challenges in academic biographical writing regarding history, memory and legacies. This thesis answers the question: Who was Mabuza and what is his place or relevance in South Africa's history? We over-simplify and stereotype people because it is convenient. We judge others but there is no saint without a past or a sinner without a future. Mabuza reconciled with the main liberation movements and moved from a maligned position on the political fringes to a respectable one within the emerging black economic elite. History, like reference systems and values is not calcified or static. It is prone to interpretation, adaptation, modification, invention, manipulation, decomposition and re-composition. Bourdieu's habitus-field analysis, theory or logic of practice, notions of capital (symbolic, political, social, cultural and economic) is used together with Latour's actor-network theory as the basis of analysis of the social contracts and trust bonds that Mabuza was able to create and which enabled him to navigate South Africa's socio-political and economic milieus during apartheid and the transition in the early 1990s. As people or actors, we believe in the mantra, nothing ventured, nothing gained. We exercise agency and take risks every day. We make choices and those choices have consequences. Mabuza's choices in the fields of education, politics and business had implications for how he is perceived or has been written into history. His choices put notions of identity, citizenship, power, legitimacy, ambition, elite accommodation, class, personal and professional networks, popular struggles, agency and structure under the spotlight. Mabuza's involvement in Bantustan politics, for instance, is contextualised in terms of a historical overview of the unpopular role played by traditional authorities in South Africa before and during colonialism and apartheid. His later foray into the world of business, however, was facilitated in part, by the personal and professional contacts that he made whilst he was in politics and the opportunities which opened up during the country's political transition. Mabuza adapted to changing circumstances and demonstrated a level of versatility which other Bantustan functionaries did not or could not exercise.
- Full Text:
- Date Issued: 2011
Time series models for paired comparisons
- Authors: Sjolander, Morne Rowan
- Date: 2011
- Subjects: Paired comparisons (Statistics)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10577 , http://hdl.handle.net/10948/d1012858
- Description: The method of paired comparisons is seen as a technique used to rank a set of objects with respect to an abstract or immeasurable property. To do this, the objects get to be compared two at a time. The results are input into a model, resulting in numbers known as weights being assigned to the objects. The weights are then used to rank the objects. The method of paired comparisons was first used for psychometric investigations. Various other applications of the method are also present, for example economic applications, and applications in sports statistics. This study involves taking paired comparison models and making them time-dependent. Not much research has been done in this area. Three new time series models for paired comparisons are created. Simulations are done to support the evidence obtained, and theoretical as well as practical examples are given to illustrate the results and to verify the efficiency of the new models. A literature study is given on the method of paired comparisons, as well as on the areas in which we apply our models. Our first two time series models for paired comparisons are the Linear-Trend Bradley- Terry Model and the Sinusoidal Bradley-Terry Model. We use the maximum likelihood approach to solve these models. We test our models using exact and randomly simulated data for various time periods and various numbers of objects. We adapt the Linear-Trend Bradley-Terry Model and received our third time series model for paired comparisons, the Log Linear-Trend Bradley-Terry Model. The daily maximum and minimum temperatures were received for Port Elizabeth, Uitenhage and Coega for 2005 until 2009. To evaluate the performance of the Linear-Trend Bradley-Terry Model and the Sinusoidal Bradley-Terry Model on estimating missing temperature data, we artificially remove observations of temperature from Coega’s temperature dataset for 2006 until 2008, and use various forms of these models to estimate the missing data points. The exchange rates for 2005 until 2008 between the following currencies: the Rand, Dollar, Euro, Pound and Yen, were obtained and various forms of our Log Linear-Trend Bradley-Terry Model are used to forecast the exchange rate for one day ahead for each month in 2006 until 2008. One of the features of this study is that we apply our time series models for paired comparisons to areas which comprise non-standard paired comparisons; and we want to encourage the use of the method of paired comparisons in a broader sense than what it is traditionally used for. The results of this study can be used in various other areas, like for example, in sports statistics, to rank the strength of sports players and predict their future scores; in Physics, to calculate weather risks of electricity generation, particularly risks related to nuclear power plants, and so forth, as well as in many other areas. It is hoped that this research will open the door to much more research in combining time series analysis with the method of paired comparisons.
- Full Text:
- Date Issued: 2011
- Authors: Sjolander, Morne Rowan
- Date: 2011
- Subjects: Paired comparisons (Statistics)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10577 , http://hdl.handle.net/10948/d1012858
- Description: The method of paired comparisons is seen as a technique used to rank a set of objects with respect to an abstract or immeasurable property. To do this, the objects get to be compared two at a time. The results are input into a model, resulting in numbers known as weights being assigned to the objects. The weights are then used to rank the objects. The method of paired comparisons was first used for psychometric investigations. Various other applications of the method are also present, for example economic applications, and applications in sports statistics. This study involves taking paired comparison models and making them time-dependent. Not much research has been done in this area. Three new time series models for paired comparisons are created. Simulations are done to support the evidence obtained, and theoretical as well as practical examples are given to illustrate the results and to verify the efficiency of the new models. A literature study is given on the method of paired comparisons, as well as on the areas in which we apply our models. Our first two time series models for paired comparisons are the Linear-Trend Bradley- Terry Model and the Sinusoidal Bradley-Terry Model. We use the maximum likelihood approach to solve these models. We test our models using exact and randomly simulated data for various time periods and various numbers of objects. We adapt the Linear-Trend Bradley-Terry Model and received our third time series model for paired comparisons, the Log Linear-Trend Bradley-Terry Model. The daily maximum and minimum temperatures were received for Port Elizabeth, Uitenhage and Coega for 2005 until 2009. To evaluate the performance of the Linear-Trend Bradley-Terry Model and the Sinusoidal Bradley-Terry Model on estimating missing temperature data, we artificially remove observations of temperature from Coega’s temperature dataset for 2006 until 2008, and use various forms of these models to estimate the missing data points. The exchange rates for 2005 until 2008 between the following currencies: the Rand, Dollar, Euro, Pound and Yen, were obtained and various forms of our Log Linear-Trend Bradley-Terry Model are used to forecast the exchange rate for one day ahead for each month in 2006 until 2008. One of the features of this study is that we apply our time series models for paired comparisons to areas which comprise non-standard paired comparisons; and we want to encourage the use of the method of paired comparisons in a broader sense than what it is traditionally used for. The results of this study can be used in various other areas, like for example, in sports statistics, to rank the strength of sports players and predict their future scores; in Physics, to calculate weather risks of electricity generation, particularly risks related to nuclear power plants, and so forth, as well as in many other areas. It is hoped that this research will open the door to much more research in combining time series analysis with the method of paired comparisons.
- Full Text:
- Date Issued: 2011
Thermal degradation of diamond compacts: a TEM investigation
- Authors: Westraadt, Johan Ewald
- Date: 2011
- Subjects: Materials -- Thermal properties Chemical weathering
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/10829 , vital:26827
- Description: Diamond compacts consist of fine diamond grains bonded together by using high pressure and high temperature. In this study transmission electron microscopy (TEM)was used to study thermal degradation of diamond compacts. Three different types of diamond compacts – namely cobalt polycrystalline diamond (PCD), calcium carbonate PCD, and diamond-SiC composites – were investigated with TEM to understand the processes that occur during synthesis. These compacts were then heated in inert atmospheres and the chemical changes studied with TEM. It was found that PCD, using cobalt as a bonding agent, will degrade after exposure to temperatures above 750ºC. The cobalt pools contain tungsten in solid solution. During heat treatment above 700ºC the solid solution tungsten combines with cobalt and dissolved carbon to form η-phase particles at the cobalt/diamond interface. At higher temperatures or insufficient tungsten levels the rate of dissolved carbon, into the cobalt pool, is too high and the excess carbon will form as graphite in the cobalt pool. Increased levels of solid solution tungsten, in the cobalt, is expected to delay the onset of graphitization in the diamond compact, thereby increasing the thermal stability of the diamond compact. Non-metallic PCD using calcium carbonate as a bonding agent was successfully sintered in this study. TEM revealed similar micro-structural features as in cobalt based PCD. No signs of thermal degradation were found after exposure to 1200ºC in vacuum for this PCD. Contaminants introduced during processing prevented a detailed study of the binder in this system. The effect of substitutional metal atoms and plastic deformation of diamond on the thermal stability of diamond-SiC composites were investigated. A piston cylinder press was developed and used to synthesize diamond-SiC composites with different levels of plastically deformed diamond. It was concluded that substitutional metal atoms and plastic deformation of diamond grains play no role in the thermal degradation of diamond compacts at 750ºC. The thermal degradation of cobalt PCD is therefore completely determined by the cobalt/diamond interaction at 750ºC.
- Full Text:
- Date Issued: 2011
- Authors: Westraadt, Johan Ewald
- Date: 2011
- Subjects: Materials -- Thermal properties Chemical weathering
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/10829 , vital:26827
- Description: Diamond compacts consist of fine diamond grains bonded together by using high pressure and high temperature. In this study transmission electron microscopy (TEM)was used to study thermal degradation of diamond compacts. Three different types of diamond compacts – namely cobalt polycrystalline diamond (PCD), calcium carbonate PCD, and diamond-SiC composites – were investigated with TEM to understand the processes that occur during synthesis. These compacts were then heated in inert atmospheres and the chemical changes studied with TEM. It was found that PCD, using cobalt as a bonding agent, will degrade after exposure to temperatures above 750ºC. The cobalt pools contain tungsten in solid solution. During heat treatment above 700ºC the solid solution tungsten combines with cobalt and dissolved carbon to form η-phase particles at the cobalt/diamond interface. At higher temperatures or insufficient tungsten levels the rate of dissolved carbon, into the cobalt pool, is too high and the excess carbon will form as graphite in the cobalt pool. Increased levels of solid solution tungsten, in the cobalt, is expected to delay the onset of graphitization in the diamond compact, thereby increasing the thermal stability of the diamond compact. Non-metallic PCD using calcium carbonate as a bonding agent was successfully sintered in this study. TEM revealed similar micro-structural features as in cobalt based PCD. No signs of thermal degradation were found after exposure to 1200ºC in vacuum for this PCD. Contaminants introduced during processing prevented a detailed study of the binder in this system. The effect of substitutional metal atoms and plastic deformation of diamond on the thermal stability of diamond-SiC composites were investigated. A piston cylinder press was developed and used to synthesize diamond-SiC composites with different levels of plastically deformed diamond. It was concluded that substitutional metal atoms and plastic deformation of diamond grains play no role in the thermal degradation of diamond compacts at 750ºC. The thermal degradation of cobalt PCD is therefore completely determined by the cobalt/diamond interaction at 750ºC.
- Full Text:
- Date Issued: 2011
The role of the mite Orthogalumna terebrantis in the biological control programme for water hyacinth, Eichhornia crassipes, in South Africa
- Authors: Marlin, Danica
- Date: 2011
- Subjects: Water hyacinth -- Biological control -- South Africa Aquatic weeds -- South Africa Invasive plants -- South Africa Mites -- South Africa Mites as biological pest control agents -- South Africa Biological pest control agents -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5762 , http://hdl.handle.net/10962/d1005450
- Description: Water hyacinth (Eichhornia crassipes) is an aquatic macrophyte originating from the Amazon basin. Due to its beautiful appearance it has been introduced into numerous countries across the world as an ornamental pond plant. It was introduced into South Africa in the early 1900s and has since reached pest proportions in many of the country’s fresh water bodies, causing significant economic and ecological losses. It is now considered to be the worst aquatic weed in South Africa. Efforts to control the spread of the weed began in the early 1970s and there have been some successes. Biological control has been used widely as an alternative to mechanical and chemical controls because it is cost-effective, self-sustaining and environmentally friendly. To date, six biological control agents have been introduced onto water hyacinth in South Africa. However, due to factors such as cold winter temperatures and interference from chemical control, the agent populations are occasionally knocked-down and thus the impact of biological control on the weed population is variable. In addition, many South African water systems are highly eutrophic, and in these systems the plant growth may be accelerated to such an extent that the negative impact of the agents’ herbivory is mitigated. One of the agents established on the weed is the galumnid mite Orthogalumna terebrantis, which originates from Uruguay. In South Africa, the mite was initially discovered on two water hyacinth infestations in the Mpumalanga Province in 1989 and it is now established at 17 sites across the country. Many biological control researchers believe that the mite is a good biological control agent but, prior to this thesis, little quantitative data existed to confirm the belief. Thus, this thesis is a post-release evaluation of O. terebrantis in which various aspects of the mite-plant relationship were investigated to determine the efficacy of the mite and thus better understand the role of the mite in the biological control programme of water hyacinth in South Africa. From laboratory experiments, in which mite densities were lower than densities occurring in the field, it was found that water hyacinth growth is largely unaffected by mite herbivory, except possibly at very high mite densities. When grown in high nutrient conditions the growth of the plant is so great that any affect the mite has is nullified. Plant growth is thus more affected by nutrients than by mite herbivory. However, mite feeding was also influenced by water nutrient levels and mite herbivory was greatest on plants grown in high nutrient conditions. The presence of the mite had a positive effect on the performance of the mirid Eccritotarsus catarinensis, such that the interactions of the two agents together had a greater negative impact on the plant’s growth than the individual agents had alone. Furthermore, water hyacinth physiological parameters, such as the plant’s photosynthetic ability, were negatively impacted by the mite, even at the very low mite densities used in the study. Plant growth rate is dependent on photosynthetic ability i.e. the rate of photosynthesis, and thus a decrease in the plant’s photosynthetic ability will eventually be translated into decreased plant growth rates which would ultimately result in the overall reduction of water hyacinth populations. In addition, temperature tolerance studies showed that the mite was tolerant of low temperatures. The mite already occurs at some of the coldest sites in South Africa. Therefore, the mite should be able to establish at all of the water hyacinth infestations in the country, but because it is a poor disperser it is unlikely to establish at new sites without human intervention. It is suggested that the mite be used as an additional biological control agent at sites where it does not yet occur, specifically at cold sites where some of the other, less cold-tolerant, agents have failed to establish. Finally, conditions of where, how many and how often the mite should be distributed to water hyacinth infestation in South Africa are discussed.
- Full Text:
- Date Issued: 2011
- Authors: Marlin, Danica
- Date: 2011
- Subjects: Water hyacinth -- Biological control -- South Africa Aquatic weeds -- South Africa Invasive plants -- South Africa Mites -- South Africa Mites as biological pest control agents -- South Africa Biological pest control agents -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5762 , http://hdl.handle.net/10962/d1005450
- Description: Water hyacinth (Eichhornia crassipes) is an aquatic macrophyte originating from the Amazon basin. Due to its beautiful appearance it has been introduced into numerous countries across the world as an ornamental pond plant. It was introduced into South Africa in the early 1900s and has since reached pest proportions in many of the country’s fresh water bodies, causing significant economic and ecological losses. It is now considered to be the worst aquatic weed in South Africa. Efforts to control the spread of the weed began in the early 1970s and there have been some successes. Biological control has been used widely as an alternative to mechanical and chemical controls because it is cost-effective, self-sustaining and environmentally friendly. To date, six biological control agents have been introduced onto water hyacinth in South Africa. However, due to factors such as cold winter temperatures and interference from chemical control, the agent populations are occasionally knocked-down and thus the impact of biological control on the weed population is variable. In addition, many South African water systems are highly eutrophic, and in these systems the plant growth may be accelerated to such an extent that the negative impact of the agents’ herbivory is mitigated. One of the agents established on the weed is the galumnid mite Orthogalumna terebrantis, which originates from Uruguay. In South Africa, the mite was initially discovered on two water hyacinth infestations in the Mpumalanga Province in 1989 and it is now established at 17 sites across the country. Many biological control researchers believe that the mite is a good biological control agent but, prior to this thesis, little quantitative data existed to confirm the belief. Thus, this thesis is a post-release evaluation of O. terebrantis in which various aspects of the mite-plant relationship were investigated to determine the efficacy of the mite and thus better understand the role of the mite in the biological control programme of water hyacinth in South Africa. From laboratory experiments, in which mite densities were lower than densities occurring in the field, it was found that water hyacinth growth is largely unaffected by mite herbivory, except possibly at very high mite densities. When grown in high nutrient conditions the growth of the plant is so great that any affect the mite has is nullified. Plant growth is thus more affected by nutrients than by mite herbivory. However, mite feeding was also influenced by water nutrient levels and mite herbivory was greatest on plants grown in high nutrient conditions. The presence of the mite had a positive effect on the performance of the mirid Eccritotarsus catarinensis, such that the interactions of the two agents together had a greater negative impact on the plant’s growth than the individual agents had alone. Furthermore, water hyacinth physiological parameters, such as the plant’s photosynthetic ability, were negatively impacted by the mite, even at the very low mite densities used in the study. Plant growth rate is dependent on photosynthetic ability i.e. the rate of photosynthesis, and thus a decrease in the plant’s photosynthetic ability will eventually be translated into decreased plant growth rates which would ultimately result in the overall reduction of water hyacinth populations. In addition, temperature tolerance studies showed that the mite was tolerant of low temperatures. The mite already occurs at some of the coldest sites in South Africa. Therefore, the mite should be able to establish at all of the water hyacinth infestations in the country, but because it is a poor disperser it is unlikely to establish at new sites without human intervention. It is suggested that the mite be used as an additional biological control agent at sites where it does not yet occur, specifically at cold sites where some of the other, less cold-tolerant, agents have failed to establish. Finally, conditions of where, how many and how often the mite should be distributed to water hyacinth infestation in South Africa are discussed.
- Full Text:
- Date Issued: 2011
The role of small antelope in ecosystem functioning in the Matobo Hills, Zimbabwe
- Authors: Lunt, Nicola
- Date: 2011
- Subjects: Antelopes -- Zimbabwe -- Rhodes Matopos National Park Ecosystem health -- Zimbabwe -- Rhodes Matopos National Park Forest ecology -- Zimbabwe -- Rhodes Matopos National Park Savanna ecology -- Zimbabwe -- Rhodes Matopos National Park Woody plants -- Effect of fires on -- Zimbabwe -- Rhodes Matopos National Park Duikers -- Zimbabwe -- Rhodes Matopos National Park Bushbuck -- Zimbabwe -- Rhodes Matopos National Park Greater kudu -- Zimbabwe -- Rhodes Matopos National Park
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5760 , http://hdl.handle.net/10962/d1005448
- Description: The 28-month study assessed the impacts of five syntopic medium-sized mammalian browsers and one fire event in a woodland savanna in the Matobo Hills, Zimbabwe. Aspects of herbivory, mechanical pressures, seed dispersal and nutrient cycling were investigated for three species of small antelope (common duiker [Sylvicapra grimmia]1, klipspringer [Oreotragus oreotragus] and steenbok [Raphicerus campestris]) and two medium-sized species (bushbuck [Tragelaphus scriptus] and greater kudu [T. strepsiceros]). Focusing on Burkea africana2 woodland, in a system that does not include elephant (Loxodonta africana), effects of browsing antelope on woody and herbaceous vegetation development were investigated using exclusion plots. Browsers regulated woody plant cover (measured as basal stem area), with smaller antelope having a greater impact than larger species. This was linked to feeding height, feeding selectivity and mechanical pressures (e.g. twig breakage and trampling). Fire caused an initial reduction in above-ground standing biomass, but in the presence of fauna, pre-fire equilibria were attained within 15 months. In antelope exclosures, herbaceous biomass increased and woody biomass decreased following fire. Responses by woody vegetation to browsing varied among species, with highly palatable species typically exhibiting compensatory regrowth. Woody species richness and abundance (especially of palatable species) increased in the absence of browsers, but species richness of the herbaceous layer was promoted by moderate disturbance (trampling or fire). Faecal deposition behaviour, primarily the use of latrines by small antelope, resulted in localised soil enrichment within defended territories. Decomposition rates (and therefore return of nutrients to the soil) varied among species and seasons, due to defecation site selection, accessibility to decomposers and desiccation rates of faecal pellets. Controlled seed germination experiments indicated that ingestion by small antelope enhances germination rates of large, hard-seeded fruits such as Sclerocarya birrea. However, germination of savanna seeds may require multiple cues. This study demonstrated the critical roles of small antelope in ecosystem functioning, and highlights the importance of the less visible impacts of frequently overlooked smaller mammalian herbivores. Perturbations to the faunal community, especially small antelope, are predicted to have substantial impacts on woody plant cover.
- Full Text:
- Date Issued: 2011
- Authors: Lunt, Nicola
- Date: 2011
- Subjects: Antelopes -- Zimbabwe -- Rhodes Matopos National Park Ecosystem health -- Zimbabwe -- Rhodes Matopos National Park Forest ecology -- Zimbabwe -- Rhodes Matopos National Park Savanna ecology -- Zimbabwe -- Rhodes Matopos National Park Woody plants -- Effect of fires on -- Zimbabwe -- Rhodes Matopos National Park Duikers -- Zimbabwe -- Rhodes Matopos National Park Bushbuck -- Zimbabwe -- Rhodes Matopos National Park Greater kudu -- Zimbabwe -- Rhodes Matopos National Park
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5760 , http://hdl.handle.net/10962/d1005448
- Description: The 28-month study assessed the impacts of five syntopic medium-sized mammalian browsers and one fire event in a woodland savanna in the Matobo Hills, Zimbabwe. Aspects of herbivory, mechanical pressures, seed dispersal and nutrient cycling were investigated for three species of small antelope (common duiker [Sylvicapra grimmia]1, klipspringer [Oreotragus oreotragus] and steenbok [Raphicerus campestris]) and two medium-sized species (bushbuck [Tragelaphus scriptus] and greater kudu [T. strepsiceros]). Focusing on Burkea africana2 woodland, in a system that does not include elephant (Loxodonta africana), effects of browsing antelope on woody and herbaceous vegetation development were investigated using exclusion plots. Browsers regulated woody plant cover (measured as basal stem area), with smaller antelope having a greater impact than larger species. This was linked to feeding height, feeding selectivity and mechanical pressures (e.g. twig breakage and trampling). Fire caused an initial reduction in above-ground standing biomass, but in the presence of fauna, pre-fire equilibria were attained within 15 months. In antelope exclosures, herbaceous biomass increased and woody biomass decreased following fire. Responses by woody vegetation to browsing varied among species, with highly palatable species typically exhibiting compensatory regrowth. Woody species richness and abundance (especially of palatable species) increased in the absence of browsers, but species richness of the herbaceous layer was promoted by moderate disturbance (trampling or fire). Faecal deposition behaviour, primarily the use of latrines by small antelope, resulted in localised soil enrichment within defended territories. Decomposition rates (and therefore return of nutrients to the soil) varied among species and seasons, due to defecation site selection, accessibility to decomposers and desiccation rates of faecal pellets. Controlled seed germination experiments indicated that ingestion by small antelope enhances germination rates of large, hard-seeded fruits such as Sclerocarya birrea. However, germination of savanna seeds may require multiple cues. This study demonstrated the critical roles of small antelope in ecosystem functioning, and highlights the importance of the less visible impacts of frequently overlooked smaller mammalian herbivores. Perturbations to the faunal community, especially small antelope, are predicted to have substantial impacts on woody plant cover.
- Full Text:
- Date Issued: 2011
The impact of toys as educative curriculum material on pre-service primary school Natural Sciences teachers' understanding of energy
- Authors: Meiring, Leslie Frank
- Date: 2011
- Subjects: Educational toys , Science -- Study and teaching (Primary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9477 , http://hdl.handle.net/10948/1332 , Educational toys , Science -- Study and teaching (Primary) -- South Africa
- Description: In this study toys were used as educative curriculum material in an intervention on the topic of energy aimed at Intermediate Phase pre-service science teachers (n= 87) registered for a BEd degree at the Nelson Mandela Metropolitan University, South Africa. The intervention consisted of, amongst other elements, lectures, assignments and toy workshops, with the latter being a key driver in the process. The choice of toys as the educative curriculum material was informed by the fact that there is a huge variety of simple, reasonably priced and easily procured toys that are suitable to demonstrate the concept of energy from both theoretical and practical perspectives. The conclusions that can be drawn from the study are that the use of toys in the preparation of pre-service primary school teachers has the potential to substantially improve their subject content knowledge (SCK), pedagogical content knowledge (PCK), and their confidence related to these two constructs with respect to their understandings of energy. The data generated also suggest that when appropriate educative curriculum materials are integrated into a topic in science-teacher education, there may be a substantial positive impact on pre-service teachers‘ confidence in both their understanding of the science content and their perceptions of their ability to teach this content. An additional finding of the study was that, despite its popularity, status and usefulness over many years, the Science Teacher Efficacy Belief Instrument (STEBI-B) shows low sensitivity to measuring changes in efficacy as a consequence of the so-called ‗ceiling effect‘. The ceiling effect is a result of initial high scores which provide little opportunity to show iii substantial positive change as a result of an appropriate intervention that is designed to improve efficacy. While this study did not directly address this lacuna in STEBI-B, it made use of an innovative descriptive statistic, ‗percentage gain of potential‘, in an attempt to describe and interpret even small changes in efficacy as measured by STEBI-B.
- Full Text:
- Date Issued: 2011
- Authors: Meiring, Leslie Frank
- Date: 2011
- Subjects: Educational toys , Science -- Study and teaching (Primary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9477 , http://hdl.handle.net/10948/1332 , Educational toys , Science -- Study and teaching (Primary) -- South Africa
- Description: In this study toys were used as educative curriculum material in an intervention on the topic of energy aimed at Intermediate Phase pre-service science teachers (n= 87) registered for a BEd degree at the Nelson Mandela Metropolitan University, South Africa. The intervention consisted of, amongst other elements, lectures, assignments and toy workshops, with the latter being a key driver in the process. The choice of toys as the educative curriculum material was informed by the fact that there is a huge variety of simple, reasonably priced and easily procured toys that are suitable to demonstrate the concept of energy from both theoretical and practical perspectives. The conclusions that can be drawn from the study are that the use of toys in the preparation of pre-service primary school teachers has the potential to substantially improve their subject content knowledge (SCK), pedagogical content knowledge (PCK), and their confidence related to these two constructs with respect to their understandings of energy. The data generated also suggest that when appropriate educative curriculum materials are integrated into a topic in science-teacher education, there may be a substantial positive impact on pre-service teachers‘ confidence in both their understanding of the science content and their perceptions of their ability to teach this content. An additional finding of the study was that, despite its popularity, status and usefulness over many years, the Science Teacher Efficacy Belief Instrument (STEBI-B) shows low sensitivity to measuring changes in efficacy as a consequence of the so-called ‗ceiling effect‘. The ceiling effect is a result of initial high scores which provide little opportunity to show iii substantial positive change as a result of an appropriate intervention that is designed to improve efficacy. While this study did not directly address this lacuna in STEBI-B, it made use of an innovative descriptive statistic, ‗percentage gain of potential‘, in an attempt to describe and interpret even small changes in efficacy as measured by STEBI-B.
- Full Text:
- Date Issued: 2011
The existence of the value premium on the Johannesburg Stock Exchange from 1972 to 2001 and extrapolation as explanation
- Authors: Beukes, Anna
- Date: 2011
- Subjects: Johannesburg Stock Exchange Stocks -- Prices -- South Africa Rational expectations (Economic theory) Investments -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:977 , http://hdl.handle.net/10962/d1002711
- Description: This study investigates the existence of the value premium in South Africa’s equity market, and tests extrapolation as a possible explanation for it. The value premium refers to the widely reported superior performance of share price returns of value companies compared to growth companies. The value premium represents an anomaly in mainstream rational finance theory, because it should not persist, unless it could be explained as the result of some composite form of risk. What is highly vexing is the fact that the value premium not only persists in most financial markets over a long period, but that the risk explanation cannot be upheld convincingly. This contributed to the rise of behavioral finance, an approach which introduces psychological factors to provide new explanations for financial phenomena. The behavioral finance explanation for the value premium observation is extrapolation (the tendency to project recent experience too far into the future). This study applies propositions and methods from behavioral finance to investigate the South African equity market. The existence of a value premium in South Africa was investigated by using twenty-nine years’ worth of accounting and share price data. The study employed one- and two-dimensional tests for portfolio formation, and tracked share price returns for up to five years after portfolio formation. The results indicated that a statistically and economically significant value premium existed in South Africa for the period between 1972 and 2001. Extrapolation as a potential explanation for the value premium observation was investigated by applying internationally used methods. Extrapolation was found to provide a robust explanation for the South African value premium.
- Full Text:
- Date Issued: 2011
- Authors: Beukes, Anna
- Date: 2011
- Subjects: Johannesburg Stock Exchange Stocks -- Prices -- South Africa Rational expectations (Economic theory) Investments -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:977 , http://hdl.handle.net/10962/d1002711
- Description: This study investigates the existence of the value premium in South Africa’s equity market, and tests extrapolation as a possible explanation for it. The value premium refers to the widely reported superior performance of share price returns of value companies compared to growth companies. The value premium represents an anomaly in mainstream rational finance theory, because it should not persist, unless it could be explained as the result of some composite form of risk. What is highly vexing is the fact that the value premium not only persists in most financial markets over a long period, but that the risk explanation cannot be upheld convincingly. This contributed to the rise of behavioral finance, an approach which introduces psychological factors to provide new explanations for financial phenomena. The behavioral finance explanation for the value premium observation is extrapolation (the tendency to project recent experience too far into the future). This study applies propositions and methods from behavioral finance to investigate the South African equity market. The existence of a value premium in South Africa was investigated by using twenty-nine years’ worth of accounting and share price data. The study employed one- and two-dimensional tests for portfolio formation, and tracked share price returns for up to five years after portfolio formation. The results indicated that a statistically and economically significant value premium existed in South Africa for the period between 1972 and 2001. Extrapolation as a potential explanation for the value premium observation was investigated by applying internationally used methods. Extrapolation was found to provide a robust explanation for the South African value premium.
- Full Text:
- Date Issued: 2011
The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies
- Authors: Goble, Jessica Leigh
- Date: 2011
- Subjects: Antimalarials -- Development Plasmodium falciparum Drug development Plasmodium falciparum -- Purification Plasmodium falciparum -- Inhibitors Enzyme kinetics Malaria -- Chemotherapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3949 , http://hdl.handle.net/10962/d1004008
- Description: Plasmodium falciparum 1–deoxy–D–xylulose–5 phosphatereductoisomerase (PfDXR) plays a role in isoprenoid biosynthesis in the malaria parasite and is absent in the human host, making this parasite enzyme an attractive target for antimalarial drug design. To characterize PfDXR, it is necessary to produce large quantities of the enzyme in a soluble and functional form. However, the over–production of malarial proteins in prokaryotic host systems often results in the formation of truncated proteins or insoluble protein aggregates. A heterologous expression system was developed for the production of active PfDXR using codon harmonization and tight control of expression in the presence of lac repressor. Yields of up to 2 mg/l of enzyme were reported using the optimised expression system, which is 8 to 10– fold greater than previously reported yields. The kinetic parameters Km, Vmax and kcat were determined for PfDXR; values reported in this study were consistent with those reported in the literature for other DXR enzymes. A three–dimensional model of the malarial drug target protein PfDXR was generated, and validated using structure–checking programs and protein docking studies. Structural and functional features unique to PfDXR were identified using the model and comparative sequence analyses with apicomplexan and non–apicomplexan DXR proteins. Residues Val44 and Asn45, essential for NADPH binding; and catalytic hatch residues Lys224 and Lys226, which are unique to the species of Plasmodium, were mutated to resemble those of E. coli DXR. Interestingly,these mutations resulted in significant reductions in substrate affinity, when compared to the unmutated PfDXR. Mutant enzymes PfDXR(VN43,44AG) and PfDXR(KK224,226NS) also demonstrated a decreased ability to turnover substrate by 4–fold and 2–fold respectively. This study indicates a difference in the role of the catalytic hatch of PfDXR with regards to the way in which it captures substrates. The study also highlights subtle differences in cofactor binding to PfDXR, compared with the well characterized EcDXR enzyme. The validated PfDXR model was also used to develop a novel efficient in silico screening method for potential tool compounds for use in the rational design of novel DXR inhibitors. Following in silico screening of 46 potential DXR inhibitors, a two–tiered in vitro screening approach was undertaken. DXR inhibition was assessed for the 46 novel compounds using an NADPH– ependant DXP enzyme inhibition assay and antimalarial potential was assessed using P.falciparum–infected erythrocyte growth assays. Select compounds were tested in human cells in order to determine whether they were toxic to the host. From the parallel in silico and in vitro drug screening, it was evident that only a single compound demonstrated reasonable potential binding to DXR (determined using in silico docking), inhibited DXR in vitro and inhibited P. falciparum growth, without being toxic to human cells. Its potential as a lead compound in antimalarial drug development is therefore feasible. Two outcomes were evident from this work. Firstly, analogues of known antimalarial natural products can be screened against malaria, which may then lead towards the rational design of novel compounds that are effective against a specific antimalarial drug target enzyme, such as PfDXR. Secondly, the rational design of novel compounds against a specific antimalarial drug target enzyme can be untaken by adopting a coupled in silico and in vitro approach to drug discovery.
- Full Text:
- Date Issued: 2011
- Authors: Goble, Jessica Leigh
- Date: 2011
- Subjects: Antimalarials -- Development Plasmodium falciparum Drug development Plasmodium falciparum -- Purification Plasmodium falciparum -- Inhibitors Enzyme kinetics Malaria -- Chemotherapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3949 , http://hdl.handle.net/10962/d1004008
- Description: Plasmodium falciparum 1–deoxy–D–xylulose–5 phosphatereductoisomerase (PfDXR) plays a role in isoprenoid biosynthesis in the malaria parasite and is absent in the human host, making this parasite enzyme an attractive target for antimalarial drug design. To characterize PfDXR, it is necessary to produce large quantities of the enzyme in a soluble and functional form. However, the over–production of malarial proteins in prokaryotic host systems often results in the formation of truncated proteins or insoluble protein aggregates. A heterologous expression system was developed for the production of active PfDXR using codon harmonization and tight control of expression in the presence of lac repressor. Yields of up to 2 mg/l of enzyme were reported using the optimised expression system, which is 8 to 10– fold greater than previously reported yields. The kinetic parameters Km, Vmax and kcat were determined for PfDXR; values reported in this study were consistent with those reported in the literature for other DXR enzymes. A three–dimensional model of the malarial drug target protein PfDXR was generated, and validated using structure–checking programs and protein docking studies. Structural and functional features unique to PfDXR were identified using the model and comparative sequence analyses with apicomplexan and non–apicomplexan DXR proteins. Residues Val44 and Asn45, essential for NADPH binding; and catalytic hatch residues Lys224 and Lys226, which are unique to the species of Plasmodium, were mutated to resemble those of E. coli DXR. Interestingly,these mutations resulted in significant reductions in substrate affinity, when compared to the unmutated PfDXR. Mutant enzymes PfDXR(VN43,44AG) and PfDXR(KK224,226NS) also demonstrated a decreased ability to turnover substrate by 4–fold and 2–fold respectively. This study indicates a difference in the role of the catalytic hatch of PfDXR with regards to the way in which it captures substrates. The study also highlights subtle differences in cofactor binding to PfDXR, compared with the well characterized EcDXR enzyme. The validated PfDXR model was also used to develop a novel efficient in silico screening method for potential tool compounds for use in the rational design of novel DXR inhibitors. Following in silico screening of 46 potential DXR inhibitors, a two–tiered in vitro screening approach was undertaken. DXR inhibition was assessed for the 46 novel compounds using an NADPH– ependant DXP enzyme inhibition assay and antimalarial potential was assessed using P.falciparum–infected erythrocyte growth assays. Select compounds were tested in human cells in order to determine whether they were toxic to the host. From the parallel in silico and in vitro drug screening, it was evident that only a single compound demonstrated reasonable potential binding to DXR (determined using in silico docking), inhibited DXR in vitro and inhibited P. falciparum growth, without being toxic to human cells. Its potential as a lead compound in antimalarial drug development is therefore feasible. Two outcomes were evident from this work. Firstly, analogues of known antimalarial natural products can be screened against malaria, which may then lead towards the rational design of novel compounds that are effective against a specific antimalarial drug target enzyme, such as PfDXR. Secondly, the rational design of novel compounds against a specific antimalarial drug target enzyme can be untaken by adopting a coupled in silico and in vitro approach to drug discovery.
- Full Text:
- Date Issued: 2011
The discoursal construction of female physical identity in selected works in children's literature
- Authors: Hunt, Sally Ann
- Date: 2011 , 2013-09-20
- Subjects: Rowling, J K -- Criticism and interpretation Lewis, C S (Clive Staples), 1898-1963 -- Criticism and interpretation Girls in literature Girls -- Identity Human body in literature Gender identity in literature Sex role in literature Children's stories, English -- History and criticism Young women in literature Women in literature
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2377 , http://hdl.handle.net/10962/d1005965
- Description: This thesis reports on an analysis of the discursive construction of female and male physical identity in children’s literature and explicitly combines corpus linguistic methods with a critical discourse approach. Based on three novels from each of the Chronicles of Narnia and the Harry Potter series, it shows clear gendering of body parts, not only in terms of the purely quantitative preferences for certain body parts to be associated with one or other gender, but in terms of discourse prosody, or the uses to which the body parts are put. Human body parts in these series are mostly used in the following four ways, all of which show differences in realisation in terms of gender: · to describe individuals, physically, in order to distinguish one from the other; · to convey emotion, unintentionally as well as consciously; · for physical interaction between people and · for interaction with the world more broadly: responses to danger and agency, i.e. the ability to act on the world and the nature of what is achieved. The use of body parts by characters to express emotion and act agentively on the world is revealed to be strongly gendered in the two series. I characterise the most prominent patterns in terms of the bodily products blood, sweat and tears, of which the last is strongly connected to female characters, who are generally associated with emotion. The other two, referring to active participation in fighting and injury, as well as agency, are almost exclusively reserved for males, with female characters rendered unable to act on the physical world as a result of overwhelming feelings. The females’ response to danger suggests stereotyped discourses of inequality which see women and girls as requiring protection and being physically incapable. Thus gender is still a particularly salient aspect in these widely-read examples of children’s literature, despite plots which appear to be fairly positive towards women. The strength of the inclusion of a corpus approach in this study lies in its capacity to reveal objective, and often fairly covert, trends in language use. These in turn enrich the critical analysis of discourses in these influential texts, which facilitates social change through linguistic analysis.
- Full Text:
- Date Issued: 2011
- Authors: Hunt, Sally Ann
- Date: 2011 , 2013-09-20
- Subjects: Rowling, J K -- Criticism and interpretation Lewis, C S (Clive Staples), 1898-1963 -- Criticism and interpretation Girls in literature Girls -- Identity Human body in literature Gender identity in literature Sex role in literature Children's stories, English -- History and criticism Young women in literature Women in literature
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2377 , http://hdl.handle.net/10962/d1005965
- Description: This thesis reports on an analysis of the discursive construction of female and male physical identity in children’s literature and explicitly combines corpus linguistic methods with a critical discourse approach. Based on three novels from each of the Chronicles of Narnia and the Harry Potter series, it shows clear gendering of body parts, not only in terms of the purely quantitative preferences for certain body parts to be associated with one or other gender, but in terms of discourse prosody, or the uses to which the body parts are put. Human body parts in these series are mostly used in the following four ways, all of which show differences in realisation in terms of gender: · to describe individuals, physically, in order to distinguish one from the other; · to convey emotion, unintentionally as well as consciously; · for physical interaction between people and · for interaction with the world more broadly: responses to danger and agency, i.e. the ability to act on the world and the nature of what is achieved. The use of body parts by characters to express emotion and act agentively on the world is revealed to be strongly gendered in the two series. I characterise the most prominent patterns in terms of the bodily products blood, sweat and tears, of which the last is strongly connected to female characters, who are generally associated with emotion. The other two, referring to active participation in fighting and injury, as well as agency, are almost exclusively reserved for males, with female characters rendered unable to act on the physical world as a result of overwhelming feelings. The females’ response to danger suggests stereotyped discourses of inequality which see women and girls as requiring protection and being physically incapable. Thus gender is still a particularly salient aspect in these widely-read examples of children’s literature, despite plots which appear to be fairly positive towards women. The strength of the inclusion of a corpus approach in this study lies in its capacity to reveal objective, and often fairly covert, trends in language use. These in turn enrich the critical analysis of discourses in these influential texts, which facilitates social change through linguistic analysis.
- Full Text:
- Date Issued: 2011
Systematics, morphology, phylogeny and historical biogeography of the Mayfly family Prosopistomatidae (Ephemeroptera: Insecta) of the world
- Barber-James, Helen Margaret
- Authors: Barber-James, Helen Margaret
- Date: 2011
- Subjects: Mayflies Mayflies -- Classification Mayflies -- Morphology Mayflies -- Phylogeny Mayflies -- Geographical distribution Ephemeridae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5785 , http://hdl.handle.net/10962/d1005473
- Description: The diversity, classification and historical biogeography of the mayfly family Prosopistomatidae are explored. First, the higher classification of the Ephemeroptera is reviewed, focussing on the phylogenetic placement of the Prosopistomatidae relative to other mayfly families. All relevant literature from 1762 to 2010 is synthesized. Baetiscidae are established as the probable sister lineage of Prosopistomatidae, the two constituting the superfamily Baetiscoidea. Next, qualitative morphological variation within the Prosopistomatidae is reviewed and revised, emphasizing nymphs because imaginal specimens are few. The labium and associated structures and the hypopharynx of nymphs, and the highly-derived wing venation of the imaginal stages, are re-interpreted. The structure of the male tarsal claws changes considerably between subimago and imago, which, together with deeply scalloped ridges on male imaginal forelegs and unusual pits on the female thorax, are interpreted as providing an unusual mating mechanism. These structures provide morphological characters for species definition and phylogenetic analyses. Two approaches to species delimitation are explored. First, morphometric variation is analysed using Principal Component Analysis, revealing groupings that can be interpreted as species, although there is some overlap between them. Discriminant Function Analysis shows that head width and carapace shape have the most value in identifying nymphs of different species. The carapace of Prosopistoma nymphs is shown to grow allometrically and gradually, in contrast with that of Baetisca, indicating a difference in early ontogeny. Second, an Artificial Neural Network algorithm applied to nymphal morphological characters accurately identified species. This computer-driven artificial intelligence method has power to provide future easy-to-use electronic identification aids. Phylogenetic analysis of nymphal morphology using the parsimony method shows two clades of Prosopistomatidae, one sharing characters with the type species, Prosopistoma variegatum and the other predominating in Africa, although also occurring in Asia; these clades are named the “P. variegatum” and “African” clades, respectively. Parsimony analysis of adult morphology supports these two clades, but supertree analysis obscures the relationships, nesting the “P. variegatum” lineage within the other clade. Preliminary molecular phylogenetic analysis of the 16S rRNA, (mitochondrial) 18S rRNA and Histone-3 genes using Bayesian Inference methods does not support the two clades shown by morphology. Instead, there is a strong relationship between the European species and one African species, with the single Asian representative being most distantly related. These results are limited by lack of fresh material, patchy taxon sampling, and problems with finding suitable primers. A molecular clock program, BEAST, calibrated using fossils, suggests divergence times for the oldest crown-group Prosopistoma clade, represented by the Asian P. wouterae, of about 131 Ma, with the youngest species, the African P. crassi, of 1.21 Ma. Stem-group relationships are analysed using parsimony analysis, focussing on wing characters of the Baetiscoidea, other extant mayfly lineages, and extinct stem-group lineages. This suggests that the Baetiscoidea diverged from main-line Ephemeroptera earlier than any other extant mayfly lineage. This approach expands upon ideas hinted at by earlier scientists. Finally, historical biogeographical analysis of the distribution of known Baetiscoidea s.s. stem-group fossils implies a once Pangean distribution of the lineage. Changing palaeo-climate, catastrophic extinction events and plate tectonic movements in relation to the distribution of crown-group species are reviewed. Other approaches to historical biogeography that build on both morphological and molecular phylogenies are used to interpret disperalist and vacarianist arguments. Distribution patterns of eight unrelated freshwater organisms which share a similar distribution pattern are compared, assuming that shared patterns indicate similar historic biogeographic processes. The distribution of recent Prosopistoma species is seen to be the product of evolution resulting from both vicariance and dispersal. In conclusion, this thesis encompasses a variety of disciplines. It successfully recognises new characters and distinguishes previously unknown species. It uses new approaches to delimiting species and known methods to determine phylogeny from several angles. The analysis of stem-group relationships offers an insight into possible early lineage splitting within Ephemeroptera. Interpretation of historical biogeography allows for both a Gondwanan origin of Prosopistomatidae, with rafting of species on the Deccan plate to Asia, and for subsequent dispersal from Asia down to Australia and across to Europe, and possibly back to Africa.
- Full Text:
- Date Issued: 2011
- Authors: Barber-James, Helen Margaret
- Date: 2011
- Subjects: Mayflies Mayflies -- Classification Mayflies -- Morphology Mayflies -- Phylogeny Mayflies -- Geographical distribution Ephemeridae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5785 , http://hdl.handle.net/10962/d1005473
- Description: The diversity, classification and historical biogeography of the mayfly family Prosopistomatidae are explored. First, the higher classification of the Ephemeroptera is reviewed, focussing on the phylogenetic placement of the Prosopistomatidae relative to other mayfly families. All relevant literature from 1762 to 2010 is synthesized. Baetiscidae are established as the probable sister lineage of Prosopistomatidae, the two constituting the superfamily Baetiscoidea. Next, qualitative morphological variation within the Prosopistomatidae is reviewed and revised, emphasizing nymphs because imaginal specimens are few. The labium and associated structures and the hypopharynx of nymphs, and the highly-derived wing venation of the imaginal stages, are re-interpreted. The structure of the male tarsal claws changes considerably between subimago and imago, which, together with deeply scalloped ridges on male imaginal forelegs and unusual pits on the female thorax, are interpreted as providing an unusual mating mechanism. These structures provide morphological characters for species definition and phylogenetic analyses. Two approaches to species delimitation are explored. First, morphometric variation is analysed using Principal Component Analysis, revealing groupings that can be interpreted as species, although there is some overlap between them. Discriminant Function Analysis shows that head width and carapace shape have the most value in identifying nymphs of different species. The carapace of Prosopistoma nymphs is shown to grow allometrically and gradually, in contrast with that of Baetisca, indicating a difference in early ontogeny. Second, an Artificial Neural Network algorithm applied to nymphal morphological characters accurately identified species. This computer-driven artificial intelligence method has power to provide future easy-to-use electronic identification aids. Phylogenetic analysis of nymphal morphology using the parsimony method shows two clades of Prosopistomatidae, one sharing characters with the type species, Prosopistoma variegatum and the other predominating in Africa, although also occurring in Asia; these clades are named the “P. variegatum” and “African” clades, respectively. Parsimony analysis of adult morphology supports these two clades, but supertree analysis obscures the relationships, nesting the “P. variegatum” lineage within the other clade. Preliminary molecular phylogenetic analysis of the 16S rRNA, (mitochondrial) 18S rRNA and Histone-3 genes using Bayesian Inference methods does not support the two clades shown by morphology. Instead, there is a strong relationship between the European species and one African species, with the single Asian representative being most distantly related. These results are limited by lack of fresh material, patchy taxon sampling, and problems with finding suitable primers. A molecular clock program, BEAST, calibrated using fossils, suggests divergence times for the oldest crown-group Prosopistoma clade, represented by the Asian P. wouterae, of about 131 Ma, with the youngest species, the African P. crassi, of 1.21 Ma. Stem-group relationships are analysed using parsimony analysis, focussing on wing characters of the Baetiscoidea, other extant mayfly lineages, and extinct stem-group lineages. This suggests that the Baetiscoidea diverged from main-line Ephemeroptera earlier than any other extant mayfly lineage. This approach expands upon ideas hinted at by earlier scientists. Finally, historical biogeographical analysis of the distribution of known Baetiscoidea s.s. stem-group fossils implies a once Pangean distribution of the lineage. Changing palaeo-climate, catastrophic extinction events and plate tectonic movements in relation to the distribution of crown-group species are reviewed. Other approaches to historical biogeography that build on both morphological and molecular phylogenies are used to interpret disperalist and vacarianist arguments. Distribution patterns of eight unrelated freshwater organisms which share a similar distribution pattern are compared, assuming that shared patterns indicate similar historic biogeographic processes. The distribution of recent Prosopistoma species is seen to be the product of evolution resulting from both vicariance and dispersal. In conclusion, this thesis encompasses a variety of disciplines. It successfully recognises new characters and distinguishes previously unknown species. It uses new approaches to delimiting species and known methods to determine phylogeny from several angles. The analysis of stem-group relationships offers an insight into possible early lineage splitting within Ephemeroptera. Interpretation of historical biogeography allows for both a Gondwanan origin of Prosopistomatidae, with rafting of species on the Deccan plate to Asia, and for subsequent dispersal from Asia down to Australia and across to Europe, and possibly back to Africa.
- Full Text:
- Date Issued: 2011
Synthesis of novel inhibitors of 1-Deoxy-D-xylulose-5-phosphate reductoisomerase as potential anti-malarial lead compounds
- Authors: Mutorwa, Marius Kudumo
- Date: 2011
- Subjects: Antimalarials -- Development Plasmodium falciparum Malaria -- Chemotherapy Drug development Lead compounds Phosphonates Phosphonic acids Ligands
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4372 , http://hdl.handle.net/10962/d1005037
- Description: This research has focused on the development of novel substrate mimics as potential DXR inhibitors of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), an essential enzyme in the mevalonate-independent pathway for the biosynthesis of isoprenoids in Plasmodium falciparum. DXR mediates the isomerisation and reduction of 1-deoxy-D-xylulose-5-phosphate (DOXP) into 2C-methyl-D-erithrytol 4-phosphate (MEP) and has been validated as an attractive target for the development of novel anti-malarial chemotherapeutic agents. Reaction of various amines with specially prepared 4-phosphonated crotonic acid in the presence of the peptide coupling reagent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), has afforded a series of amido-phosphonate esters in moderate to good yields (48% - 73%) which, using a RuCl₃/CeCl₃/NaIO₄ catalyst system, have been dihydroxylated to furnish the dihydroxy-amido phosphonate ester pro-drugs; subsequent hydrolysis under microwave irradiation has afforded the corresponding phosphonic acids. A second series of potential inhibitors viz., 3-substituted aniline-derived phosphonate esters, their corresponding phosphonic acids and mono-sodium salts, have also been successfully synthesised. In these compounds, the essential functional groups are separated by one, two, three or four methylene groups, Deprotonation of the 3-substituted aniline substrates, followed by reaction with the appropriate ω-chloroalkanoyl chloride produced the ω-chloroamide intermediates, which were subjected to the Michaelis-Arbuzov reaction to afford the diethyl phosphonate esters in moderate to good yields (48% - 74%). Microwave-assisted TMSBrmediated cleavage of the phosphonate esters furnished the phosphonic acids, neutralisation of which afforded the mono-sodium salts. Furan-derived phosphate esters and phosphonic acids have been prepared as conformationally-restricted DOXP analogues. Functionalization at C-5 of the trityl-protected furan was achieved using the Vilsmeier-Haack formylation and Friedel-Crafts acylation reactions and, following de-tritylation, phosphorylation and oximation, using hydroxylamine hydrochloride, the novel oxime derivatives have been isolated as a third series of potential DXR inhibitors in very good yields (87% - 96%). Finally, in order to exploit an additional binding pocket in the PƒDXR active site, a series of N-benzylated phosphoramidic derivatives were obtained in seven steps from the starting material, diethyl phosphoramidate. The known inhibitors, fosmidomycin and its acetyl derivative FR900098, were also successfully synthesised as standards for STD-NMR binding and inhibition assays. In all, over 200 compounds (136 novel) have been prepared and appropriately characterised using 1-and 2-D NMR and IR spectroscopic analysis and, where necessary, HRMS or combustion analysis. Saturation Transfer Difference (STD) protein-NMR experiments, undertaken using selected compounds, have revealed binding of most of the ligands examined to EcDXR. Computersimulated docking studies have also been used to explore the preferred ligand-binding conformations and interactions between the ligands and essential DXR active-site residues, while DXR-enzyme inhibition assays of selected synthesised ligands have revealed certain patterns of inhibitory activity.
- Full Text:
- Date Issued: 2011
- Authors: Mutorwa, Marius Kudumo
- Date: 2011
- Subjects: Antimalarials -- Development Plasmodium falciparum Malaria -- Chemotherapy Drug development Lead compounds Phosphonates Phosphonic acids Ligands
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4372 , http://hdl.handle.net/10962/d1005037
- Description: This research has focused on the development of novel substrate mimics as potential DXR inhibitors of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), an essential enzyme in the mevalonate-independent pathway for the biosynthesis of isoprenoids in Plasmodium falciparum. DXR mediates the isomerisation and reduction of 1-deoxy-D-xylulose-5-phosphate (DOXP) into 2C-methyl-D-erithrytol 4-phosphate (MEP) and has been validated as an attractive target for the development of novel anti-malarial chemotherapeutic agents. Reaction of various amines with specially prepared 4-phosphonated crotonic acid in the presence of the peptide coupling reagent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), has afforded a series of amido-phosphonate esters in moderate to good yields (48% - 73%) which, using a RuCl₃/CeCl₃/NaIO₄ catalyst system, have been dihydroxylated to furnish the dihydroxy-amido phosphonate ester pro-drugs; subsequent hydrolysis under microwave irradiation has afforded the corresponding phosphonic acids. A second series of potential inhibitors viz., 3-substituted aniline-derived phosphonate esters, their corresponding phosphonic acids and mono-sodium salts, have also been successfully synthesised. In these compounds, the essential functional groups are separated by one, two, three or four methylene groups, Deprotonation of the 3-substituted aniline substrates, followed by reaction with the appropriate ω-chloroalkanoyl chloride produced the ω-chloroamide intermediates, which were subjected to the Michaelis-Arbuzov reaction to afford the diethyl phosphonate esters in moderate to good yields (48% - 74%). Microwave-assisted TMSBrmediated cleavage of the phosphonate esters furnished the phosphonic acids, neutralisation of which afforded the mono-sodium salts. Furan-derived phosphate esters and phosphonic acids have been prepared as conformationally-restricted DOXP analogues. Functionalization at C-5 of the trityl-protected furan was achieved using the Vilsmeier-Haack formylation and Friedel-Crafts acylation reactions and, following de-tritylation, phosphorylation and oximation, using hydroxylamine hydrochloride, the novel oxime derivatives have been isolated as a third series of potential DXR inhibitors in very good yields (87% - 96%). Finally, in order to exploit an additional binding pocket in the PƒDXR active site, a series of N-benzylated phosphoramidic derivatives were obtained in seven steps from the starting material, diethyl phosphoramidate. The known inhibitors, fosmidomycin and its acetyl derivative FR900098, were also successfully synthesised as standards for STD-NMR binding and inhibition assays. In all, over 200 compounds (136 novel) have been prepared and appropriately characterised using 1-and 2-D NMR and IR spectroscopic analysis and, where necessary, HRMS or combustion analysis. Saturation Transfer Difference (STD) protein-NMR experiments, undertaken using selected compounds, have revealed binding of most of the ligands examined to EcDXR. Computersimulated docking studies have also been used to explore the preferred ligand-binding conformations and interactions between the ligands and essential DXR active-site residues, while DXR-enzyme inhibition assays of selected synthesised ligands have revealed certain patterns of inhibitory activity.
- Full Text:
- Date Issued: 2011
Synthesis and evaluation of novel HIV-1 enzyme inhibitors
- Olomola, Temitope Oloruntoba
- Authors: Olomola, Temitope Oloruntoba
- Date: 2011
- Subjects: HIV infections -- Treatment HIV infections -- Chemotherapy HIV (Viruses) Enzyme inhibitors AZT (Drug) Reverse transcriptase Proteolytic enzymes Ligands Psoralens Resorcinol
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4369 , http://hdl.handle.net/10962/d1005034
- Description: This study has involved the design, synthesis and evaluation of novel HIV-1 enzyme inhibitors accessed by synthetic elaboration of Baylis-Hillman adducts. Several series of complex coumarin-AZT and cinnamate ester-AZT conjugates have been prepared, in high yields, by exploiting the click reaction between appropriate Baylis-Hillman derived precursors and azidothymidine (AZT), all of which have been fully characterised using spectroscopic techniques. These conjugates, designed as potential dual-action HIV-1 inhibitors, were tested against the appropriate HIV-1 enzymes, i.e. HIV-1 reverse transcriptase and protease or HIV-1 reverse transcriptase and integrase. A number of the ligands have exhibited % inhibition levels and IC50 values comparable to drugs in clinical use, permitting their identification as lead compounds for the development of novel dual-action inhibitors. In silico docking of selected ligands into the active sites of the respective enzymes has provided useful insight into binding conformations and potential hydrogen-bonding interactions with active-site amino acid residues. A series of furocoumarin carboxamide derivatives have been synthesised in four steps starting from resorcinol and these compounds have also been tested for HIV-1 integrase inhibition activity. The structures of unexpected products isolated from Aza-Baylis-Hillman reactions of N-tosylaldimines have been elucidated by spectroscopic analysis, and confirmed by single crystal X-ray analysis. A mechanism for what appears to be an unprecedented transformation has been proposed. Microwave-assisted SeO₂ oxidation of Baylis-Hillman-derived 3-methylcoumarins has provided convenient and efficient access to coumarin-3-carbaldehydes, and a pilot study has revealed the potential of these coumarin-3-carbaldehydes as scaffolds for the construction of tricyclic compounds. The HCl-catalysed reaction of tert-butyl acrylate derived Baylis-Hillman adducts has been shown to afford 3-(chloromethyl)coumarins and α-(chloromethyl)cinnamic acids, the Zstereochemistry of the latter being established by X-ray crystallography. ¹H NMR-based experimental kinetic and DFT-level theoretical studies have been undertaken to establish the reaction sequence and other mechanistic details. Base-catalysed cyclisation on the other hand, has been shown to afford 2H-chromene rather than coumarin derivatives.
- Full Text:
- Date Issued: 2011
- Authors: Olomola, Temitope Oloruntoba
- Date: 2011
- Subjects: HIV infections -- Treatment HIV infections -- Chemotherapy HIV (Viruses) Enzyme inhibitors AZT (Drug) Reverse transcriptase Proteolytic enzymes Ligands Psoralens Resorcinol
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4369 , http://hdl.handle.net/10962/d1005034
- Description: This study has involved the design, synthesis and evaluation of novel HIV-1 enzyme inhibitors accessed by synthetic elaboration of Baylis-Hillman adducts. Several series of complex coumarin-AZT and cinnamate ester-AZT conjugates have been prepared, in high yields, by exploiting the click reaction between appropriate Baylis-Hillman derived precursors and azidothymidine (AZT), all of which have been fully characterised using spectroscopic techniques. These conjugates, designed as potential dual-action HIV-1 inhibitors, were tested against the appropriate HIV-1 enzymes, i.e. HIV-1 reverse transcriptase and protease or HIV-1 reverse transcriptase and integrase. A number of the ligands have exhibited % inhibition levels and IC50 values comparable to drugs in clinical use, permitting their identification as lead compounds for the development of novel dual-action inhibitors. In silico docking of selected ligands into the active sites of the respective enzymes has provided useful insight into binding conformations and potential hydrogen-bonding interactions with active-site amino acid residues. A series of furocoumarin carboxamide derivatives have been synthesised in four steps starting from resorcinol and these compounds have also been tested for HIV-1 integrase inhibition activity. The structures of unexpected products isolated from Aza-Baylis-Hillman reactions of N-tosylaldimines have been elucidated by spectroscopic analysis, and confirmed by single crystal X-ray analysis. A mechanism for what appears to be an unprecedented transformation has been proposed. Microwave-assisted SeO₂ oxidation of Baylis-Hillman-derived 3-methylcoumarins has provided convenient and efficient access to coumarin-3-carbaldehydes, and a pilot study has revealed the potential of these coumarin-3-carbaldehydes as scaffolds for the construction of tricyclic compounds. The HCl-catalysed reaction of tert-butyl acrylate derived Baylis-Hillman adducts has been shown to afford 3-(chloromethyl)coumarins and α-(chloromethyl)cinnamic acids, the Zstereochemistry of the latter being established by X-ray crystallography. ¹H NMR-based experimental kinetic and DFT-level theoretical studies have been undertaken to establish the reaction sequence and other mechanistic details. Base-catalysed cyclisation on the other hand, has been shown to afford 2H-chromene rather than coumarin derivatives.
- Full Text:
- Date Issued: 2011
Surface properties and electrocatalytic applications of metallophthalocyanines confined on electrode surfaces
- Authors: Akinbulu, Isaac Adebayo
- Date: 2011
- Subjects: Phthalocyanines Electrochemistry Electrocatalysis Pesticides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4365 , http://hdl.handle.net/10962/d1005030
- Description: New cobalt (13, 16 19 and 22), manganese (14, 17, 20 and 23) and iron (15, 18, 21 and 24)phthalocyanine complexes were synthesized and characterized. The UV-Vis spectral properties of the complexes were typical of the nature of central metal and position of substituent on the Pc ligand. Their electrochemical behaviors were signatures of the central metals, with varying influences of the nature and position of substituents. Nanocomposite of complex 18 and single walled carbon nanotubes (SWCNTs) (SWCNT-18)was fabricated. Formation of this nano-composite was confirmed by infrared (IR)spectroscopy, X-ray diffraction (XRD) spectroscopy and transmission electron microscopy (TEM). Self-assembled monolayers (SAMs) of SWCNT-18, complexes 13-15, and 20 were electropolymerized on glassy carbon electrodes (GCE). Complex 14 was also electrodeposited on GCE. Surface properties of the SAMs were consistent with the molecular feature of the substituent and the nature of central metal in the adsorbed species, while those of the MnPc modified GCEs were dependent on point of substitution and number of substituent. The SAM-modified gold electrodes were used for the electrocatalytic oxidation of the carbamate insecticide, carbofuran. Amplification of the current signal of the insecticide, at more energetically feasible oxidation potentials, on the SAM-modified gold electrodes, relative to bare gold electrode,justified electrocatalysis. There was enhanced sensitivity (attributed to the presence of SWCNT) of the SWCNT-18-SAM-modified gold electrode towards carbofuran, relative to the signals observed on the other SAMs. Current response of the insecticide,bendiocarb, was also intensified, at more favorable oxidation potentials, on the MnPc (14 and 17) modified GCEs, relative to the response on bare GCE, substantiating electrocatalysis. Also, catalysis of the oxidation of the herbicide, bentazon, was observed on polymeric film of complex 20. The current response of the herbicide on this film was better than that observed on bare GCE. Electrocatalysis of the analytes, on the respective modified electrodes, occurred via closely related mechanisms.
- Full Text:
- Date Issued: 2011
- Authors: Akinbulu, Isaac Adebayo
- Date: 2011
- Subjects: Phthalocyanines Electrochemistry Electrocatalysis Pesticides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4365 , http://hdl.handle.net/10962/d1005030
- Description: New cobalt (13, 16 19 and 22), manganese (14, 17, 20 and 23) and iron (15, 18, 21 and 24)phthalocyanine complexes were synthesized and characterized. The UV-Vis spectral properties of the complexes were typical of the nature of central metal and position of substituent on the Pc ligand. Their electrochemical behaviors were signatures of the central metals, with varying influences of the nature and position of substituents. Nanocomposite of complex 18 and single walled carbon nanotubes (SWCNTs) (SWCNT-18)was fabricated. Formation of this nano-composite was confirmed by infrared (IR)spectroscopy, X-ray diffraction (XRD) spectroscopy and transmission electron microscopy (TEM). Self-assembled monolayers (SAMs) of SWCNT-18, complexes 13-15, and 20 were electropolymerized on glassy carbon electrodes (GCE). Complex 14 was also electrodeposited on GCE. Surface properties of the SAMs were consistent with the molecular feature of the substituent and the nature of central metal in the adsorbed species, while those of the MnPc modified GCEs were dependent on point of substitution and number of substituent. The SAM-modified gold electrodes were used for the electrocatalytic oxidation of the carbamate insecticide, carbofuran. Amplification of the current signal of the insecticide, at more energetically feasible oxidation potentials, on the SAM-modified gold electrodes, relative to bare gold electrode,justified electrocatalysis. There was enhanced sensitivity (attributed to the presence of SWCNT) of the SWCNT-18-SAM-modified gold electrode towards carbofuran, relative to the signals observed on the other SAMs. Current response of the insecticide,bendiocarb, was also intensified, at more favorable oxidation potentials, on the MnPc (14 and 17) modified GCEs, relative to the response on bare GCE, substantiating electrocatalysis. Also, catalysis of the oxidation of the herbicide, bentazon, was observed on polymeric film of complex 20. The current response of the herbicide on this film was better than that observed on bare GCE. Electrocatalysis of the analytes, on the respective modified electrodes, occurred via closely related mechanisms.
- Full Text:
- Date Issued: 2011
Studies in marine quinone chemistry
- Authors: Sunassee, Suthananda Naidu
- Date: 2011
- Subjects: Quinone Marine natural products Marine invertebrates Marine pharmacology Cancer -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4355 , http://hdl.handle.net/10962/d1005020
- Description: This thesis is divided into two parts and the rationale of the research conducted is based on the cytotoxicity of the prenylated quinones 1.24-1.29, isolated from the South African nudibranch Leminda millecra, against oesophageal cancer cells. The first part (Chapters 2 and 3) of the thesis initially documents the distribution of cytotoxic and antioxidant prenylated quinones and hydroquinones in the marine environment. We have been able to show, for the first time, that these compounds can be divided into eight structural classes closely related to their phyletic distribution. Secondly, we attempted to synthesize the two marine natural products 1.24 and 1.26 in an effort to contribute to an ongoing collaborative search with the Division of Medical Biochemistry at the University of Cape Town for new compounds with anti-oesophageal cancer activity. Accordingly, we followed the published synthetic procedure for 1.26 and, although we were unable to reproduce the reported results, we have generated five new prenylated quinone analogues 3.53-3.55, 3.63 and 3.71, which are a potentially viable addition to our ongoing structure-activity relationship (SAR) studies. Moreover, we embarked on a 7Li NMR mechanistic study for the synthesis of 3.2 from 3.1 which rewarded us with an improved and reproducible methodology for this crucial reaction that is detailed in Chapter 3. The second part of this thesis (Chapters 4 and 5) is concerned with a synthetic, structural, electrochemical and biological exploration of the 1,4-naphthoquinone nucleus as a primary pharmacophore in our search for new chemical entities which can induce apoptosis in oesophageal cancer cells, thus contributing to our overall ongoing SAR study in this class of compounds. Seven new naphthoquinone derivatves (4.19, 4.30, 4.31, 4.33 and 4.46-4.48) of the natural products 2-deoxylapachol (2.44), lapachol (4.1) and β-lapachone (5.2) were synthesized and 2-(1`-hydroxy-`-phenylmethyl)-1,4-naphthoquinone (4.29) was found to be the most cytotoxic (IC50 1.5 μM) against the oesophageal cancer cell line WHCO1, while 5.2, which is currently in phase II clinical trials as an anticancer drug, was found to be similarly active (IC50 1.6 μM). Electrochemical investigations of the redox properties of the benzylic alcohol derivatives 4.29-4.31 indicated a higher reduction potential compared to their oxidized counterparts 4.45-4.48, and this finding has been correlated to the increased activity of 4.29-4.31 against the WHCO1 cell line. Additionally, 4.29 is synthetically more accessible than either 1.26 or 5.2 and potentially a lead compound in our search for new and more effective chemotherapeutic agents against oesophageal cancer
- Full Text:
- Date Issued: 2011
- Authors: Sunassee, Suthananda Naidu
- Date: 2011
- Subjects: Quinone Marine natural products Marine invertebrates Marine pharmacology Cancer -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4355 , http://hdl.handle.net/10962/d1005020
- Description: This thesis is divided into two parts and the rationale of the research conducted is based on the cytotoxicity of the prenylated quinones 1.24-1.29, isolated from the South African nudibranch Leminda millecra, against oesophageal cancer cells. The first part (Chapters 2 and 3) of the thesis initially documents the distribution of cytotoxic and antioxidant prenylated quinones and hydroquinones in the marine environment. We have been able to show, for the first time, that these compounds can be divided into eight structural classes closely related to their phyletic distribution. Secondly, we attempted to synthesize the two marine natural products 1.24 and 1.26 in an effort to contribute to an ongoing collaborative search with the Division of Medical Biochemistry at the University of Cape Town for new compounds with anti-oesophageal cancer activity. Accordingly, we followed the published synthetic procedure for 1.26 and, although we were unable to reproduce the reported results, we have generated five new prenylated quinone analogues 3.53-3.55, 3.63 and 3.71, which are a potentially viable addition to our ongoing structure-activity relationship (SAR) studies. Moreover, we embarked on a 7Li NMR mechanistic study for the synthesis of 3.2 from 3.1 which rewarded us with an improved and reproducible methodology for this crucial reaction that is detailed in Chapter 3. The second part of this thesis (Chapters 4 and 5) is concerned with a synthetic, structural, electrochemical and biological exploration of the 1,4-naphthoquinone nucleus as a primary pharmacophore in our search for new chemical entities which can induce apoptosis in oesophageal cancer cells, thus contributing to our overall ongoing SAR study in this class of compounds. Seven new naphthoquinone derivatves (4.19, 4.30, 4.31, 4.33 and 4.46-4.48) of the natural products 2-deoxylapachol (2.44), lapachol (4.1) and β-lapachone (5.2) were synthesized and 2-(1`-hydroxy-`-phenylmethyl)-1,4-naphthoquinone (4.29) was found to be the most cytotoxic (IC50 1.5 μM) against the oesophageal cancer cell line WHCO1, while 5.2, which is currently in phase II clinical trials as an anticancer drug, was found to be similarly active (IC50 1.6 μM). Electrochemical investigations of the redox properties of the benzylic alcohol derivatives 4.29-4.31 indicated a higher reduction potential compared to their oxidized counterparts 4.45-4.48, and this finding has been correlated to the increased activity of 4.29-4.31 against the WHCO1 cell line. Additionally, 4.29 is synthetically more accessible than either 1.26 or 5.2 and potentially a lead compound in our search for new and more effective chemotherapeutic agents against oesophageal cancer
- Full Text:
- Date Issued: 2011
Structural and functional characterisation of the protein inhibitor of activated STAT3 (PIAS3)
- Authors: Mautsa, Nicodemus
- Date: 2011
- Subjects: Cytokines Immune response Proteins Cancer cells -- Growth -- Regulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3991 , http://hdl.handle.net/10962/d1004050
- Description: The signal transducer and activator of transcription (STAT) and protein inhibitor of STAT(PIAS) system represent an elegant regulatory mechanism of transcriptional control IN mammalian cytokine signalling. Abnormal activation of the system is associated with immune disorders and a large group of diverse tumours. PIAS3 is a multiple domain protein with distinct functions involved in regulation of cytokine-mediated gene activation pathways.Its over-expression significantly inhibits cell growth and renders cancer cells more sensitive to drugs. The objective of this study was to structurally and biochemically characterise the function of the PIAS3 protein using in silico, in vivo and in vitro analysis approaches.The conservation pattern of the PIAS protein family and critical conserved residues in the PINIT (Proline, Isoleucine, Asparagine, Isoleucine, Tyrosine) domain were identified. The PINIT domain model was generated based on the PINIT domain structure of yeast PIAS3 homologue Siz1 and structural determinants in the PIAS3-STAT3 interaction were evaluated.Guided by the in silico findings, in vivo analysis of the localisation of the PIAS3, mutantderivatives of PIAS3 (PIAS3-L97A, PIAS3-R99N, PIAS3-R99Q), PINIT and acidic domain was conducted. PIAS3 was completely localised in the nucleus while PIAS3 mutants appeared to exhibit diffuse cytoplasmic distribution. The PINIT domain was predominantly localised in the nucleus with some apparent perinuclear staining while the acidic domain exhibited a predominantly perinuclear staining pattern. Further analysis of the PINIT domain and the effect of the mutants on PIAS3-STAT3 interaction were assessed by in vitro analysis. Guided by in silico analysis, the PINIT domain and mutant derivatives of PINIT domain (PINIT-L97A, PINIT-R99N, and PINIT-R99Q) were heterologously expressed in Escherichia coli and subsequently purified using a combination of immobilized metal affinity and size exclusion based chromatography. The size and structural elements of the PINIT domain and its mutants were characterised. The 23 kDa PINIT domain was found to exist as a monomer in solution and its secondary structure was shown to consist of 66 % β-sheets by fourier transformed infrared spectroscopy consistent with the generated homology model.Using surface plasmonresonance spectroscopy (SPR) the PINIT domain was shown to bind to STAT3 in a specific concentration dependent manner. Recombinant PINIT-L97A,PINITR99N and PINIT-R99Q mutants, which exhibited similar structural integrity to the wildtype, were found to abrogate binding to STAT3. These findings suggest that these residues form part of a potential binding surface for stat3. In conclusion, this study has provided evidence that the PINIT domain is an important determinant of PIAS3 interaction with STAT3 and that the interaction is mediated by defined conserved residues directly involved in the PINITSTAT3 interaction.
- Full Text:
- Date Issued: 2011
- Authors: Mautsa, Nicodemus
- Date: 2011
- Subjects: Cytokines Immune response Proteins Cancer cells -- Growth -- Regulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3991 , http://hdl.handle.net/10962/d1004050
- Description: The signal transducer and activator of transcription (STAT) and protein inhibitor of STAT(PIAS) system represent an elegant regulatory mechanism of transcriptional control IN mammalian cytokine signalling. Abnormal activation of the system is associated with immune disorders and a large group of diverse tumours. PIAS3 is a multiple domain protein with distinct functions involved in regulation of cytokine-mediated gene activation pathways.Its over-expression significantly inhibits cell growth and renders cancer cells more sensitive to drugs. The objective of this study was to structurally and biochemically characterise the function of the PIAS3 protein using in silico, in vivo and in vitro analysis approaches.The conservation pattern of the PIAS protein family and critical conserved residues in the PINIT (Proline, Isoleucine, Asparagine, Isoleucine, Tyrosine) domain were identified. The PINIT domain model was generated based on the PINIT domain structure of yeast PIAS3 homologue Siz1 and structural determinants in the PIAS3-STAT3 interaction were evaluated.Guided by the in silico findings, in vivo analysis of the localisation of the PIAS3, mutantderivatives of PIAS3 (PIAS3-L97A, PIAS3-R99N, PIAS3-R99Q), PINIT and acidic domain was conducted. PIAS3 was completely localised in the nucleus while PIAS3 mutants appeared to exhibit diffuse cytoplasmic distribution. The PINIT domain was predominantly localised in the nucleus with some apparent perinuclear staining while the acidic domain exhibited a predominantly perinuclear staining pattern. Further analysis of the PINIT domain and the effect of the mutants on PIAS3-STAT3 interaction were assessed by in vitro analysis. Guided by in silico analysis, the PINIT domain and mutant derivatives of PINIT domain (PINIT-L97A, PINIT-R99N, and PINIT-R99Q) were heterologously expressed in Escherichia coli and subsequently purified using a combination of immobilized metal affinity and size exclusion based chromatography. The size and structural elements of the PINIT domain and its mutants were characterised. The 23 kDa PINIT domain was found to exist as a monomer in solution and its secondary structure was shown to consist of 66 % β-sheets by fourier transformed infrared spectroscopy consistent with the generated homology model.Using surface plasmonresonance spectroscopy (SPR) the PINIT domain was shown to bind to STAT3 in a specific concentration dependent manner. Recombinant PINIT-L97A,PINITR99N and PINIT-R99Q mutants, which exhibited similar structural integrity to the wildtype, were found to abrogate binding to STAT3. These findings suggest that these residues form part of a potential binding surface for stat3. In conclusion, this study has provided evidence that the PINIT domain is an important determinant of PIAS3 interaction with STAT3 and that the interaction is mediated by defined conserved residues directly involved in the PINITSTAT3 interaction.
- Full Text:
- Date Issued: 2011
Strategies to create a post-merged organisational culture conducive to effective performance management
- Authors: Paul, Gary William
- Date: 2011
- Subjects: Corporate culture , Organizational change , Organizational effectiveness
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9385 , http://hdl.handle.net/10948/d1010857 , Corporate culture , Organizational change , Organizational effectiveness
- Description: Mergers have been described as the most complex business process that an organisation can be faced with, requiring executives and other stakeholders to discharge the promise of a more successful merged organisation. However, several studies have highlighted the factors that led to the demise of the merged organisations. One of the often quoted and frequently blamed aspects related to merger failure has been the lack of effective post-merged organisational culture integration and alignment. Where mergers have been successful, it was attributed to a structured approach to integrating and aligning all aspects related to organisational culture thus ensuring the creation of a high performing organisation, conducive to effective performance management. The main research problem in this study centred around the identification of strategies that could be used to design an integrated model for creating a post-merged organisational culture which is conducive to effectively managing performance. To achieve this objective, the following approaches were adopted: A literature study was conducted with the view to identifying the challenges facing merged organisations in general and post-merged South African Higher Education institutions in particular. The researcher also conducted interviews with senior HR practitioner at the institutions participating in this study to gain insights into their experiences of performance within their merged institutions. The institutions involved in this study were Nelson Mandela Metropolitan University (NMMU), Cape Peninsula University of Technology (CPUT and Durban University of Technology (DUT). The insights gleaned from these interviews were incorporated into the survey questionnaire. The literature study also concerned itself with the identification of strategies that merged organisations could use in its pursuit of organisational culture alignment and integration. These strategies included conducting critical pre-merger assessments or due-diligence studies, adopting structured approaches to dealing with resistance to change, employee engagement, the design, implementation and communication of monitoring and evaluation of merger success measures as well as several other moderating variables referred to in figure 1.2. In terms of sub-problem six of the study, the findings of sub-problems one, two and five were used to develop an eight-step integrated theoretical model to create an organisational culture conducive to effective performance management in a post-merged environment. The model served as a basis for the design of a survey questionnaire. The questionnaire was used to ascertain the extent to which respondents from the three participating institutions (NMMU, CPUT and DUT), perceived the various strategies as being important in establishing a post-merged organisational culture conducive to effective performance management. The results that emerged from the empirical study showed a strong concurrence with the strategies identified in the literature study and included in the integrated theoretical model. The quantitative and qualitative results from the empirical study where incorporated into the integrated theoretical model, which lead to a refined Eight-Step Integrated Post-merged Organisational Culture Creation Model as depicted in Figure 7.1 with associated details in Figure 7.2.
- Full Text:
- Date Issued: 2011
- Authors: Paul, Gary William
- Date: 2011
- Subjects: Corporate culture , Organizational change , Organizational effectiveness
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9385 , http://hdl.handle.net/10948/d1010857 , Corporate culture , Organizational change , Organizational effectiveness
- Description: Mergers have been described as the most complex business process that an organisation can be faced with, requiring executives and other stakeholders to discharge the promise of a more successful merged organisation. However, several studies have highlighted the factors that led to the demise of the merged organisations. One of the often quoted and frequently blamed aspects related to merger failure has been the lack of effective post-merged organisational culture integration and alignment. Where mergers have been successful, it was attributed to a structured approach to integrating and aligning all aspects related to organisational culture thus ensuring the creation of a high performing organisation, conducive to effective performance management. The main research problem in this study centred around the identification of strategies that could be used to design an integrated model for creating a post-merged organisational culture which is conducive to effectively managing performance. To achieve this objective, the following approaches were adopted: A literature study was conducted with the view to identifying the challenges facing merged organisations in general and post-merged South African Higher Education institutions in particular. The researcher also conducted interviews with senior HR practitioner at the institutions participating in this study to gain insights into their experiences of performance within their merged institutions. The institutions involved in this study were Nelson Mandela Metropolitan University (NMMU), Cape Peninsula University of Technology (CPUT and Durban University of Technology (DUT). The insights gleaned from these interviews were incorporated into the survey questionnaire. The literature study also concerned itself with the identification of strategies that merged organisations could use in its pursuit of organisational culture alignment and integration. These strategies included conducting critical pre-merger assessments or due-diligence studies, adopting structured approaches to dealing with resistance to change, employee engagement, the design, implementation and communication of monitoring and evaluation of merger success measures as well as several other moderating variables referred to in figure 1.2. In terms of sub-problem six of the study, the findings of sub-problems one, two and five were used to develop an eight-step integrated theoretical model to create an organisational culture conducive to effective performance management in a post-merged environment. The model served as a basis for the design of a survey questionnaire. The questionnaire was used to ascertain the extent to which respondents from the three participating institutions (NMMU, CPUT and DUT), perceived the various strategies as being important in establishing a post-merged organisational culture conducive to effective performance management. The results that emerged from the empirical study showed a strong concurrence with the strategies identified in the literature study and included in the integrated theoretical model. The quantitative and qualitative results from the empirical study where incorporated into the integrated theoretical model, which lead to a refined Eight-Step Integrated Post-merged Organisational Culture Creation Model as depicted in Figure 7.1 with associated details in Figure 7.2.
- Full Text:
- Date Issued: 2011
Sedimentology of plio-pleistocene gravel barrier deposits in the palaeo-Orange River mouth, Namibia : depositional history and diamond mineralisation
- Authors: Spaggiari, Renato Igino
- Date: 2011 , 2013-08-19
- Subjects: Diamond mines and mining -- Namibia Sediments (Geology) -- South Africa and Namibia -- Orange River Estuary Diamond deposits -- Namibia Orange River Estuary (Namibia and South Africa)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4927 , http://hdl.handle.net/10962/d1004636
- Description: The largest known marine diamond placer, the Namibian mega-placer, lies along the Atlantic coast of south-western Africa from the Orange River mouth 1,000 km northwards to the Namibian-Angolan border. The most economically viable portion of the Namibian mega-placer (>75 million carats recovered at >95% gem quality) comprises onshore and offshore marine deposits that are developed within ∼100km of the Orange River outfall. For much of the Cainozoic, this long-lived fluvial system has been the main conduit transporting diamonds from kimberlitic and secondary sources in the cratonic hinterland of southern Africa to the Atlantic shelf that has been neutrally buoyant over this period. Highly energetic marine processes, driven in part, by southerly winds with an attendant northward-directed longshore drift, have generated terminal placers that are preserved both onshore and offshore. This study, through detailed field sedimentological and diamond analyses, investigates the development and mineralisation of gravel barrier deposits within the ancestral Orange River mouth area during a major ∼30 m regional transgression ('30 m Package') in the Late Pliocene. At that time, diamond supply from this fluvial conduit was minimal, yet the corresponding onshore marine deposits to the north of the Orange River mouth were significantly diamond enriched, enabling large-scale alluvial diamond mining to take place for over 75 years. Of the entire coastline of south-western Africa, the most complete accumulation of the '30 m Package' is preserved within the palaeo-Orange River mouth as barrier spit and barrier beach deposits. Arranged vertically and laterally in a 16m thick succession, these are deposits of: (1) intertidal beach, (2) lagoon and washover, (3) tidal inlet and spit recurve and (4) storm-dominated subtidal settings. These were parts of larger barrier features, the bulk of which are preserved as highstand deposits that are diamond-bearing with varying, but generally low grades (<13 stones (diamonds) per hundred tons, spht). Intertidal beach and spit recurve deposits have higher economic grades (12-13 spht) due to the energetic sieving and mobile trapping mechanisms associated with their emplacement. In contrast, the less reworked and more sandy subtidal, tidal inlet and washover deposits have un-economic grades (<2 spht). Despite these low grades, the barrier deposits have the largest average stone (diamond) size (1-2 carats/stone, cts/stn) of the entire Namibian mega-placer, given their proximity to the ancestral Orange River outfall. This study demonstrates that barrier shoreline evolution at the fluvial/marine interface was controlled by: (1) a strong and coarse fluvial sediment supply that sustained shoreline growth on a highly energetic coast, (2) accommodation space facilitating sediment preservation and (3) short-duration, high-frequency sea-level cycles superimposed on the∼30 m regional transgression, promoting hierarchal stacking of progradational deposits. During these sea-level fluctuations, diamonds were 'farmed' from older, shelf sequences in the offshore and driven landward to accumulate in '30 m Package' highstand barrier deposits. In spite of the large supply of diamonds, their retention in these deposits was poor due to an incompetent footwall of ancestral Orange River mouth sediment and the inherent cobble-boulder size of the barrier gravels. Thus the principal process controlling diamond entrapment in these barrier deposits was kinetic sieving in a coarse-grained framework. Consequently, at the marine/fluvial interface and down-drift for ∼5 km, larger diamonds (1-2 cts/stn) were retained in low-grade (<2 spht), coarse-gravel barrier shorelines. Smaller diamonds (mostly < I cts/stn) were rejected into the northward-driven littoral sediments and further size-sorted along ∼95 km of Namibian coast to accumulate in finer, high-grade beach placers (> 100 spht) where bedrock footwall promoted such high concentrations. The gravel-dominated palaeo-Orange River mouth is considered to be the ' heart' of the Namibian mega-placer, controlling sediment and diamond supply to the littoral zone further north. Although coarse gravel is retained at the river mouth, the incompetence of this highly energetic setting to trap diamonds renders it sub-economic. This ineffectiveness at the fluvial/marine interface is thus fundamental in enriching the coastal tract farther down-drift and developing highly economic coastal placers along the Atlantic coast of south-western Africa. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2011
- Authors: Spaggiari, Renato Igino
- Date: 2011 , 2013-08-19
- Subjects: Diamond mines and mining -- Namibia Sediments (Geology) -- South Africa and Namibia -- Orange River Estuary Diamond deposits -- Namibia Orange River Estuary (Namibia and South Africa)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4927 , http://hdl.handle.net/10962/d1004636
- Description: The largest known marine diamond placer, the Namibian mega-placer, lies along the Atlantic coast of south-western Africa from the Orange River mouth 1,000 km northwards to the Namibian-Angolan border. The most economically viable portion of the Namibian mega-placer (>75 million carats recovered at >95% gem quality) comprises onshore and offshore marine deposits that are developed within ∼100km of the Orange River outfall. For much of the Cainozoic, this long-lived fluvial system has been the main conduit transporting diamonds from kimberlitic and secondary sources in the cratonic hinterland of southern Africa to the Atlantic shelf that has been neutrally buoyant over this period. Highly energetic marine processes, driven in part, by southerly winds with an attendant northward-directed longshore drift, have generated terminal placers that are preserved both onshore and offshore. This study, through detailed field sedimentological and diamond analyses, investigates the development and mineralisation of gravel barrier deposits within the ancestral Orange River mouth area during a major ∼30 m regional transgression ('30 m Package') in the Late Pliocene. At that time, diamond supply from this fluvial conduit was minimal, yet the corresponding onshore marine deposits to the north of the Orange River mouth were significantly diamond enriched, enabling large-scale alluvial diamond mining to take place for over 75 years. Of the entire coastline of south-western Africa, the most complete accumulation of the '30 m Package' is preserved within the palaeo-Orange River mouth as barrier spit and barrier beach deposits. Arranged vertically and laterally in a 16m thick succession, these are deposits of: (1) intertidal beach, (2) lagoon and washover, (3) tidal inlet and spit recurve and (4) storm-dominated subtidal settings. These were parts of larger barrier features, the bulk of which are preserved as highstand deposits that are diamond-bearing with varying, but generally low grades (<13 stones (diamonds) per hundred tons, spht). Intertidal beach and spit recurve deposits have higher economic grades (12-13 spht) due to the energetic sieving and mobile trapping mechanisms associated with their emplacement. In contrast, the less reworked and more sandy subtidal, tidal inlet and washover deposits have un-economic grades (<2 spht). Despite these low grades, the barrier deposits have the largest average stone (diamond) size (1-2 carats/stone, cts/stn) of the entire Namibian mega-placer, given their proximity to the ancestral Orange River outfall. This study demonstrates that barrier shoreline evolution at the fluvial/marine interface was controlled by: (1) a strong and coarse fluvial sediment supply that sustained shoreline growth on a highly energetic coast, (2) accommodation space facilitating sediment preservation and (3) short-duration, high-frequency sea-level cycles superimposed on the∼30 m regional transgression, promoting hierarchal stacking of progradational deposits. During these sea-level fluctuations, diamonds were 'farmed' from older, shelf sequences in the offshore and driven landward to accumulate in '30 m Package' highstand barrier deposits. In spite of the large supply of diamonds, their retention in these deposits was poor due to an incompetent footwall of ancestral Orange River mouth sediment and the inherent cobble-boulder size of the barrier gravels. Thus the principal process controlling diamond entrapment in these barrier deposits was kinetic sieving in a coarse-grained framework. Consequently, at the marine/fluvial interface and down-drift for ∼5 km, larger diamonds (1-2 cts/stn) were retained in low-grade (<2 spht), coarse-gravel barrier shorelines. Smaller diamonds (mostly < I cts/stn) were rejected into the northward-driven littoral sediments and further size-sorted along ∼95 km of Namibian coast to accumulate in finer, high-grade beach placers (> 100 spht) where bedrock footwall promoted such high concentrations. The gravel-dominated palaeo-Orange River mouth is considered to be the ' heart' of the Namibian mega-placer, controlling sediment and diamond supply to the littoral zone further north. Although coarse gravel is retained at the river mouth, the incompetence of this highly energetic setting to trap diamonds renders it sub-economic. This ineffectiveness at the fluvial/marine interface is thus fundamental in enriching the coastal tract farther down-drift and developing highly economic coastal placers along the Atlantic coast of south-western Africa. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2011