A study of the properties and methods of analysis of high molecular weight N-nitrosamines
- Authors: Kelly, Felix Thomas
- Date: 1974 , 2013-10-29
- Subjects: Nitrosoamines , Chemistry, Physical and theoretical
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4421 , http://hdl.handle.net/10962/d1006878 , Nitrosoamines , Chemistry, Physical and theoretical
- Description: Various high molecular weight dialkylnitrosamines were prepared including, for the first time, methyl-n-octadecylnitrosamine and di-n-dodecylnitrosamine. The infrared, ultraviolet and mass spectra of a selection of these compounds were recorded and studied. Gel permeation chromatography was used for the isolation of individual nitrosamines in standard nitrosamine mixtures, while ion-exchange chromatography effected complete clean-up of amine-contaminated nitrosamine solutions. Thin-layer and gas-liquid chromatographic methods were developed for the detection, separation and analysis of nanogram quantities of these lipophilic nitrosamines. In addition the above chromatographic systems were used for the analysis of distillates of spiked wheat flour samples. High recoveries of dicyclopentylnitrosamine, di-n-heptylnitrosamine and di-n-octylnitrosamine, from the spiked wheat flour samples, were achieved using a specially developed freeze-drying/vacuum distillation technique, the distillates obtained being relatively free from major contaminants. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1974
- Authors: Kelly, Felix Thomas
- Date: 1974 , 2013-10-29
- Subjects: Nitrosoamines , Chemistry, Physical and theoretical
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4421 , http://hdl.handle.net/10962/d1006878 , Nitrosoamines , Chemistry, Physical and theoretical
- Description: Various high molecular weight dialkylnitrosamines were prepared including, for the first time, methyl-n-octadecylnitrosamine and di-n-dodecylnitrosamine. The infrared, ultraviolet and mass spectra of a selection of these compounds were recorded and studied. Gel permeation chromatography was used for the isolation of individual nitrosamines in standard nitrosamine mixtures, while ion-exchange chromatography effected complete clean-up of amine-contaminated nitrosamine solutions. Thin-layer and gas-liquid chromatographic methods were developed for the detection, separation and analysis of nanogram quantities of these lipophilic nitrosamines. In addition the above chromatographic systems were used for the analysis of distillates of spiked wheat flour samples. High recoveries of dicyclopentylnitrosamine, di-n-heptylnitrosamine and di-n-octylnitrosamine, from the spiked wheat flour samples, were achieved using a specially developed freeze-drying/vacuum distillation technique, the distillates obtained being relatively free from major contaminants. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 1974
Effects of precipitating electrons in the ionosphere
- Authors: Haschick, Aubrey D
- Date: 1974
- Subjects: Electrons , Electron precipitation , Ionosphere , Ionospheric electron density
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5526 , http://hdl.handle.net/10962/d1012220
- Description: As early as 1896, around the time of the discovery of the electron by J.J. Thompson, Birkeland was led to propose that aurorae were caused by fast moving electrons or similarly charged particles emitted by the sun and 'sucked in towards' the auroral zones by the geomagnetic field. He later supported this idea by firing electrons at a dipole field surrounding a sphere covered with a fluorescent coating. Extensive ground based observations of auroral features eventually led, in 1950, to the initial direct evidence of the fact that auroral emissions are due to energetic charged particles, consisting partly of protons, entering the earth's atmosphere (Meinel, 1951). However, it was only in 1952 and 1953 that the first measurements of what was later interpreted as bremsstrahlung X- rays from precipitating electrons were made at auroral latitudes. (Meredith et aI, 1955) During the IGY, 1957 - 1958, a number of rockets were fired through and near, visible aurorae and large fluxes of low energy electrons were detected ... Intro., p. 1.
- Full Text:
- Date Issued: 1974
- Authors: Haschick, Aubrey D
- Date: 1974
- Subjects: Electrons , Electron precipitation , Ionosphere , Ionospheric electron density
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5526 , http://hdl.handle.net/10962/d1012220
- Description: As early as 1896, around the time of the discovery of the electron by J.J. Thompson, Birkeland was led to propose that aurorae were caused by fast moving electrons or similarly charged particles emitted by the sun and 'sucked in towards' the auroral zones by the geomagnetic field. He later supported this idea by firing electrons at a dipole field surrounding a sphere covered with a fluorescent coating. Extensive ground based observations of auroral features eventually led, in 1950, to the initial direct evidence of the fact that auroral emissions are due to energetic charged particles, consisting partly of protons, entering the earth's atmosphere (Meinel, 1951). However, it was only in 1952 and 1953 that the first measurements of what was later interpreted as bremsstrahlung X- rays from precipitating electrons were made at auroral latitudes. (Meredith et aI, 1955) During the IGY, 1957 - 1958, a number of rockets were fired through and near, visible aurorae and large fluxes of low energy electrons were detected ... Intro., p. 1.
- Full Text:
- Date Issued: 1974
Physico-chemical and substructural studies on Nudaurelia capensis β virus
- Authors: Struthers, J Keith
- Date: 1974
- Subjects: Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4075 , http://hdl.handle.net/10962/d1007327 , Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Description: From Introduction: The pine emperor moth, Nudaurelia cytherea capensis Stoll is an insect which, during the larval stage, causes extensive defoliation of the pine tree, Pinus radiata in the Cape province. These insects are susceptible to a virus disease, which on occasions causes large scale mortality. Five nonoccluded viruses have been shown to infect the pine emperor moth, and of these, one found in the greatest concentration, Nudaurelia capensis β virus (NβV) has been characterised to the greatest extent. This virus has been shown to contain RNA, to be isometric with a diameter of 36 mm, and to have a molecular weight of 16 million. The virus occurs in all stages of the insect's development, and by fluorescent antibody staining has been shown to develop in the cytoplasm of the host's cells. There have in recent years been a number of reports describing nonoccluded RNA viruses which appear to be similar to NβV. These are the viruses isolated from the moths Gonometa podocarpi and Antheraea eucalypti, and the one from the citrus red mite, Panonychus citri. These viruses have not been as extensively characterised as NβV, so the extent of the similarity between them and NβV is not known. However it would appear as if their discovery collectively heralds the emergence of a distinct new grouping within the nonoccluded RNA viruses of insects. This work reports the isolation and further characterisation of N. capensis β virus, its protein and nucleic acid.
- Full Text:
- Date Issued: 1974
- Authors: Struthers, J Keith
- Date: 1974
- Subjects: Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4075 , http://hdl.handle.net/10962/d1007327 , Imbrasia cytherea , Insects -- Viruses , RNA viruses , DNA
- Description: From Introduction: The pine emperor moth, Nudaurelia cytherea capensis Stoll is an insect which, during the larval stage, causes extensive defoliation of the pine tree, Pinus radiata in the Cape province. These insects are susceptible to a virus disease, which on occasions causes large scale mortality. Five nonoccluded viruses have been shown to infect the pine emperor moth, and of these, one found in the greatest concentration, Nudaurelia capensis β virus (NβV) has been characterised to the greatest extent. This virus has been shown to contain RNA, to be isometric with a diameter of 36 mm, and to have a molecular weight of 16 million. The virus occurs in all stages of the insect's development, and by fluorescent antibody staining has been shown to develop in the cytoplasm of the host's cells. There have in recent years been a number of reports describing nonoccluded RNA viruses which appear to be similar to NβV. These are the viruses isolated from the moths Gonometa podocarpi and Antheraea eucalypti, and the one from the citrus red mite, Panonychus citri. These viruses have not been as extensively characterised as NβV, so the extent of the similarity between them and NβV is not known. However it would appear as if their discovery collectively heralds the emergence of a distinct new grouping within the nonoccluded RNA viruses of insects. This work reports the isolation and further characterisation of N. capensis β virus, its protein and nucleic acid.
- Full Text:
- Date Issued: 1974
Studies on anaerobic R factor transfer in facultative and anaerobic enteric bacteria
- Authors: Moodie, Hildegard Laura
- Date: 1974
- Subjects: Anaerobic bacteria , R factors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4252 , http://hdl.handle.net/10962/d1007684 , Anaerobic bacteria , R factors
- Description: Introduction: R factor mediated transfer of antibiotic resistance between Enterobacteriaceae has been reported to occur in the mammalian gastrointestinal tract (Farrar et al, 1972; Guinée, 1970; Kasuya, 1964; Reed et al, 1969; Wiedemann et al, 1970). In vivo conjugal transfer of genetic material has also been demonstrated with F¹, F⁺ and Hfr Escherichia coli strains (Jones & Curtiss, 1970). The environment in the lower gastrointestinal tract, where bacteria are abundant, is mainly anaerobic. This is demonstrated by the dominance of obligately anaerobic bacteria such as Bacteroides species (Finegold, 1969; Moore et al, 1969) and direct studies of intestinal gas composition (Askevold, 1956). However, most laboratory investigations of the incidence of R factors and their transfer frequencies have been performed under aerobic conditions using faecal facultative strains. The only investigation of resistance transfer under anaerobic conditions in vitro is that of Mitsuhashi (1965), who reported complete inhibition of transfer of an R factor from a Shigella flexneri donor to an Escherichia coli recipient. In addition, Fisher (1957) reported restriction of chromosomal transfer by an E. coli Hfr strain under anaerobic conditions in various media. On the basis of these results, it could be questioned whether in vivo R factor transfer is in fact possible (Chabbert et al, 1969). The contradictory situation prompted a reexamination of conjugation in facultative strains under anaerobic conditions. Both Fisher (1957) and Mitsuhashi (1965) obtained anaerobic conditions by evacuation. In this investigation, both mating and selection of recombinants were performed under stringent anaerobic conditions using methods developed for the isolation of obligate anaerobes (Hungate, 1969) to obtain a degree of anaerobiosis similar to that found in vivo.
- Full Text:
- Date Issued: 1974
- Authors: Moodie, Hildegard Laura
- Date: 1974
- Subjects: Anaerobic bacteria , R factors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4252 , http://hdl.handle.net/10962/d1007684 , Anaerobic bacteria , R factors
- Description: Introduction: R factor mediated transfer of antibiotic resistance between Enterobacteriaceae has been reported to occur in the mammalian gastrointestinal tract (Farrar et al, 1972; Guinée, 1970; Kasuya, 1964; Reed et al, 1969; Wiedemann et al, 1970). In vivo conjugal transfer of genetic material has also been demonstrated with F¹, F⁺ and Hfr Escherichia coli strains (Jones & Curtiss, 1970). The environment in the lower gastrointestinal tract, where bacteria are abundant, is mainly anaerobic. This is demonstrated by the dominance of obligately anaerobic bacteria such as Bacteroides species (Finegold, 1969; Moore et al, 1969) and direct studies of intestinal gas composition (Askevold, 1956). However, most laboratory investigations of the incidence of R factors and their transfer frequencies have been performed under aerobic conditions using faecal facultative strains. The only investigation of resistance transfer under anaerobic conditions in vitro is that of Mitsuhashi (1965), who reported complete inhibition of transfer of an R factor from a Shigella flexneri donor to an Escherichia coli recipient. In addition, Fisher (1957) reported restriction of chromosomal transfer by an E. coli Hfr strain under anaerobic conditions in various media. On the basis of these results, it could be questioned whether in vivo R factor transfer is in fact possible (Chabbert et al, 1969). The contradictory situation prompted a reexamination of conjugation in facultative strains under anaerobic conditions. Both Fisher (1957) and Mitsuhashi (1965) obtained anaerobic conditions by evacuation. In this investigation, both mating and selection of recombinants were performed under stringent anaerobic conditions using methods developed for the isolation of obligate anaerobes (Hungate, 1969) to obtain a degree of anaerobiosis similar to that found in vivo.
- Full Text:
- Date Issued: 1974
The Aapiesboomen magnesite deposit, Burgersfort, Transvaal
- Authors: Taylor, N C
- Date: 1974
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:21130 , http://hdl.handle.net/10962/6505
- Description: The published literature on magnesite is extensively reviewed as regards the properties, uses, production, occurrence and genesis of the mineral. Despite opposition from the producers of sea water magnesia in recent years, magnesite still finds a ready market for the manufacture of refractory materials, particularly in the steel industry. Less important uses include the manufacture of magnesium metal, carbonic acid gas, Sorel cement, paper, pharmaceuticals and fertilizers. Large bodies of cryptocrystalline magnesite occur in Greece, Yugoslavia, Turkey and India, while the deposits in Austria, Czechoslovakia, Russia and China produce most of the world supply of crystalline material. Four types of magnesite bodies may be distinguished: quartz-magnesite deposits, talc-magnesite occurrences, magnesite associated with sedimentary rocks and sagvandite, a magnesite-bronzite rock. Most South African deposits, including the Aapiesboomen body, belong to the first type. Experimental, field and textural evidence indicates that the quartz-magnesite deposits may form from serpentine under a wide range of hydrothermal and supergene conditions below about 400°C and between very low and very high concentrations of co2 in the fluid phase. Above 400°C talc is produced at the expense of quartz. Sedimentary magnesite has been observed t o form in present day saline lakes, but the large size of the older crystalline deposits associated with limestones and dolomites has been used as an argument against the use of the same principle to explain the genesis of the latter bodies : much controversy still surrounds the sedimentary-hydrothermal debate concerning their origin. It seems probable thet sagvandites are produced by a process of CO2- metasomatism.
- Full Text:
- Date Issued: 1974
- Authors: Taylor, N C
- Date: 1974
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:21130 , http://hdl.handle.net/10962/6505
- Description: The published literature on magnesite is extensively reviewed as regards the properties, uses, production, occurrence and genesis of the mineral. Despite opposition from the producers of sea water magnesia in recent years, magnesite still finds a ready market for the manufacture of refractory materials, particularly in the steel industry. Less important uses include the manufacture of magnesium metal, carbonic acid gas, Sorel cement, paper, pharmaceuticals and fertilizers. Large bodies of cryptocrystalline magnesite occur in Greece, Yugoslavia, Turkey and India, while the deposits in Austria, Czechoslovakia, Russia and China produce most of the world supply of crystalline material. Four types of magnesite bodies may be distinguished: quartz-magnesite deposits, talc-magnesite occurrences, magnesite associated with sedimentary rocks and sagvandite, a magnesite-bronzite rock. Most South African deposits, including the Aapiesboomen body, belong to the first type. Experimental, field and textural evidence indicates that the quartz-magnesite deposits may form from serpentine under a wide range of hydrothermal and supergene conditions below about 400°C and between very low and very high concentrations of co2 in the fluid phase. Above 400°C talc is produced at the expense of quartz. Sedimentary magnesite has been observed t o form in present day saline lakes, but the large size of the older crystalline deposits associated with limestones and dolomites has been used as an argument against the use of the same principle to explain the genesis of the latter bodies : much controversy still surrounds the sedimentary-hydrothermal debate concerning their origin. It seems probable thet sagvandites are produced by a process of CO2- metasomatism.
- Full Text:
- Date Issued: 1974
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