Phloem loading in the sucrose-export-defective (SXD-1) mutant maize is limited by callose deposition at plasmodesmata in bundle sheath-vascular parenchyma interface
- Botha, Christiaan E J, Cross, Robin H M, Van Bel, A J E, Peter, Craig I
- Authors: Botha, Christiaan E J , Cross, Robin H M , Van Bel, A J E , Peter, Craig I
- Date: 2000
- Language: English
- Type: Article
- Identifier: vital:6503 , http://hdl.handle.net/10962/d1005926
- Description: Using Lucifer Yellow we have demonstrated that the phloem-loading pathway from the mesophyll to the bundle sheath-vascular parenchyma interface in Zea mays source leaves follows a symplasmic route in small and intermediate vascular bundles in control as well as in the green sections of mutant sucrose-export-defective (SXD-1) plants. In the anthocyanin-rich mutant leaf sections, Lucifer Yellow transport was prohibited along the same path, at the bundle sheath-vascular parenchyma interface in particular. Plasmodesmata at the latter interface in SXD-1 anthocyanin-rich leaf sections appear to be structurally altered through callose deposition at the plasmodesmal orifices. We suggest that a transport bottleneck at the bundle sheath-vascular parenchyma interface is thus orchestrated and regulated through callose formation, preventing symplasmic transport across this important loading interface.
- Full Text:
- Date Issued: 2000
- Authors: Botha, Christiaan E J , Cross, Robin H M , Van Bel, A J E , Peter, Craig I
- Date: 2000
- Language: English
- Type: Article
- Identifier: vital:6503 , http://hdl.handle.net/10962/d1005926
- Description: Using Lucifer Yellow we have demonstrated that the phloem-loading pathway from the mesophyll to the bundle sheath-vascular parenchyma interface in Zea mays source leaves follows a symplasmic route in small and intermediate vascular bundles in control as well as in the green sections of mutant sucrose-export-defective (SXD-1) plants. In the anthocyanin-rich mutant leaf sections, Lucifer Yellow transport was prohibited along the same path, at the bundle sheath-vascular parenchyma interface in particular. Plasmodesmata at the latter interface in SXD-1 anthocyanin-rich leaf sections appear to be structurally altered through callose deposition at the plasmodesmal orifices. We suggest that a transport bottleneck at the bundle sheath-vascular parenchyma interface is thus orchestrated and regulated through callose formation, preventing symplasmic transport across this important loading interface.
- Full Text:
- Date Issued: 2000
An empirical formula for estimating the water use of Scaevola plumieri
- Peter, Craig I, Ripley, Bradley S
- Authors: Peter, Craig I , Ripley, Bradley S
- Date: 2000
- Language: English
- Type: Article
- Identifier: vital:6530 , http://hdl.handle.net/10962/d1005971
- Description: Transpirational water loss of Scaevola plumieri (L) Vahl. (= Scaevola thunbergii Eckl. & Zeyh.) (Goodeniaceae), a dune pioneer plant along the eastern and southern coasts of South Africa, was measured over a wide range of atmospheric conditions and related to the atmospheric vapour pressure deficit (VPD). A plot of leaf transpiration rate (E) against VPD yielded a curvilinear relationship (r[sup 2] = 0.88, P = 0.000). As both leaf and canopy E were found to be closely coupled to atmospheric conditions, this correlation was used to calculate canopy E for entire days. The bulk volumes of water transpired per day were related to the mean daily temperature as recorded at a nearby weather station and were strongly related (r2 = 0.71, P = 0.0037). This correlation allowed the bulk volume of water transpired by one square metre of a S. plumieri-covered dune to be calculated on a daily basis for an entire year. Transpirational losses (566 litres) in 1997 were then compared with rainfall (539 litres), indicating that rainfall was perhaps insufficient to support the plants that year. Similar deficits were calculated for 1996, suggesting that plants were possibly reliant on water stored in the sand or taped ground water. This species showed an unusual positive response of increasing leaf conductance to increasing VPD over the range 0-3 kPa.
- Full Text: false
- Date Issued: 2000
- Authors: Peter, Craig I , Ripley, Bradley S
- Date: 2000
- Language: English
- Type: Article
- Identifier: vital:6530 , http://hdl.handle.net/10962/d1005971
- Description: Transpirational water loss of Scaevola plumieri (L) Vahl. (= Scaevola thunbergii Eckl. & Zeyh.) (Goodeniaceae), a dune pioneer plant along the eastern and southern coasts of South Africa, was measured over a wide range of atmospheric conditions and related to the atmospheric vapour pressure deficit (VPD). A plot of leaf transpiration rate (E) against VPD yielded a curvilinear relationship (r[sup 2] = 0.88, P = 0.000). As both leaf and canopy E were found to be closely coupled to atmospheric conditions, this correlation was used to calculate canopy E for entire days. The bulk volumes of water transpired per day were related to the mean daily temperature as recorded at a nearby weather station and were strongly related (r2 = 0.71, P = 0.0037). This correlation allowed the bulk volume of water transpired by one square metre of a S. plumieri-covered dune to be calculated on a daily basis for an entire year. Transpirational losses (566 litres) in 1997 were then compared with rainfall (539 litres), indicating that rainfall was perhaps insufficient to support the plants that year. Similar deficits were calculated for 1996, suggesting that plants were possibly reliant on water stored in the sand or taped ground water. This species showed an unusual positive response of increasing leaf conductance to increasing VPD over the range 0-3 kPa.
- Full Text: false
- Date Issued: 2000
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