Characterisation of two novel ferrocenyl benzoxazines as in vitro triple-negative breast cancer inhibitors
- Authors: Mhlanga, Richwell
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/365689 , vital:65776
- Description: Thesis access embargoed. Expected released date early 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
- Authors: Mhlanga, Richwell
- Date: 2022-10-14
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/365689 , vital:65776
- Description: Thesis access embargoed. Expected released date early 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-10-14
Ectomycorrhizal fungal assessment of South African Pinus patula seedlings and their biological control potential to enhance seedling growth
- FitzGerald, Véronique Chartier
- Authors: FitzGerald, Véronique Chartier
- Date: 2019
- Subjects: Ectomycorrhizal fungi , Pinus patula Diseases and pests Biological control South Africa , Fusarium , Forest nurseries , Seedlings Growth , Seedlings Diseases and pests
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/67902 , vital:29167
- Description: The South Africa forestry industry, covering 1.3 million hectares, is dependent on exotic pine and eucalyptus species. Nursery seedlings are not inoculated with ectomycorrhizal (ECM) fungi or other beneficial microbes. Fusarium circinatum is an economically important pathogen affecting seedling survival. The purpose of this investigation was to assess levels of naturally occurring ECM colonisation in South African nurseries and to determine the effects of seedling inoculation with selected ECM and bacterial isolates on plant growth and resistance to the fungal pathogen F. circiantum. Pinus patula seedlings from 10 different South African nurseries were assessed for ECM colonisation using a grid line intersect method and molecularly identified using morphological and next-generation Illumina sequencing. Explants from ECM basidiocarps, collected from Pinus stands, were plated onto MMN medium to obtain isolates which were verified using molecular techniques. Mycorrhizal helper bacteria (MHB) were also isolated from these basiocarps, tested for MHB properties, siderophore production, phospahte solubilising and IAA production. ECM and associated bacterial isolates were used to inoculate seedlings and growth was assessed over a 5 month period. Colonisation of seedlings in production nurseries was low (2-21%). Morphologically the ECM fungi T. terrestris, Suillus sibiricus, and the genera Russula, Pseudotomentella were identified. Molecularly the ECM fungi T. terrestris, Inocybe jacobi and the genera Sphaerosporella and several other ECM containing families were identified along with many saprotrophic/endophytic fungi belonging to genera such as, Penicillium, Ramasonia and Talaromyces. Inoculated seedlings showed a significant increase in growth in comparison to the un-inoculated control seedlings. ECM fungal colonisation levels of these seedlings were significantly increased and colonisation was promoted by the Suillus isolate, Salmon Suillus. Seedling growth in the presence of the pathogen F. circinatum was significantly increased and promoted by the Lactarius isolate Lactarius quieticolor. Inoculation of seedlings in the nursery would ensure the production of stronger healthy plants which may be more tolerant to fusarial infection increasing survival in the plantation. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
- Authors: FitzGerald, Véronique Chartier
- Date: 2019
- Subjects: Ectomycorrhizal fungi , Pinus patula Diseases and pests Biological control South Africa , Fusarium , Forest nurseries , Seedlings Growth , Seedlings Diseases and pests
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/67902 , vital:29167
- Description: The South Africa forestry industry, covering 1.3 million hectares, is dependent on exotic pine and eucalyptus species. Nursery seedlings are not inoculated with ectomycorrhizal (ECM) fungi or other beneficial microbes. Fusarium circinatum is an economically important pathogen affecting seedling survival. The purpose of this investigation was to assess levels of naturally occurring ECM colonisation in South African nurseries and to determine the effects of seedling inoculation with selected ECM and bacterial isolates on plant growth and resistance to the fungal pathogen F. circiantum. Pinus patula seedlings from 10 different South African nurseries were assessed for ECM colonisation using a grid line intersect method and molecularly identified using morphological and next-generation Illumina sequencing. Explants from ECM basidiocarps, collected from Pinus stands, were plated onto MMN medium to obtain isolates which were verified using molecular techniques. Mycorrhizal helper bacteria (MHB) were also isolated from these basiocarps, tested for MHB properties, siderophore production, phospahte solubilising and IAA production. ECM and associated bacterial isolates were used to inoculate seedlings and growth was assessed over a 5 month period. Colonisation of seedlings in production nurseries was low (2-21%). Morphologically the ECM fungi T. terrestris, Suillus sibiricus, and the genera Russula, Pseudotomentella were identified. Molecularly the ECM fungi T. terrestris, Inocybe jacobi and the genera Sphaerosporella and several other ECM containing families were identified along with many saprotrophic/endophytic fungi belonging to genera such as, Penicillium, Ramasonia and Talaromyces. Inoculated seedlings showed a significant increase in growth in comparison to the un-inoculated control seedlings. ECM fungal colonisation levels of these seedlings were significantly increased and colonisation was promoted by the Suillus isolate, Salmon Suillus. Seedling growth in the presence of the pathogen F. circinatum was significantly increased and promoted by the Lactarius isolate Lactarius quieticolor. Inoculation of seedlings in the nursery would ensure the production of stronger healthy plants which may be more tolerant to fusarial infection increasing survival in the plantation. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
Identification of potential inhibitors of the folate biosynthesis enzymes HPPK of Salmonella enterica and Escherichia coli and pteridine reductase of Trypanosoma brucei through molecular docking and enzyme assays
- Authors: Gerwel, Tiaan Marc
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192419 , vital:45224
- Description: Thesis (MSc (Pharm)) -- Faculty of Pharmacy, Pharmacy, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Gerwel, Tiaan Marc
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192419 , vital:45224
- Description: Thesis (MSc (Pharm)) -- Faculty of Pharmacy, Pharmacy, 2021
- Full Text:
- Date Issued: 2021-10-29
The perceived socio-environmental impacts of illegal sand mining along the Umvoti River in KwaDukuza Local Municipality, KwaZulu - Natal Province, South Africa
- Authors: Mbiko, Lubabalo
- Date: 2020-07
- Subjects: Ecosystem health , Biodiversity
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20474 , vital:45669
- Description: This study examined and documented the perceptions of local communities towards the socio-environmental impacts associated with illegal sand mining activities along the Umvoti River, in the KwaDukuza Local Municipality in South Africa. It was imperative to elicit the perceptions of both the local people and the government officials to understand the extent of the socio- environmental effects of illegal sand mining in the study area. To this end, a qualitative research approach was designed and used to collect the data. Data collection techniques employed included site observations and a semi-structured interview schedule with key informants. Additionally, 50 questionnaire surveys were administered to the residents of the Nsikeni and Melville communities within the study area. The results of the study revealed that illegal sand mining along the Umvoti River has contributed significantly to the degradation of grazing and farm lands, including diversion of the river and the destruction of the riparian vegetation. Evidence of water pollution, and air pollution in the form of dust, were also reported. The study observed problems in ecosystem functioning, particularly in regard to the flora and fauna, and there was extensive destruction of the aesthetic beauty of the local landscape. In addition, the study indicated that people and livestock drowned in the deep trenches that were not rehabilitated following illegal sand mining practise that was migrating along the river. Furthermore, illegal sand mining was linked with an increase in poverty and prostitution, as well as the destruction of the infrastructure in the nearby communities. Moreover, community conflicts arose from the differing perceptions on the existence of illegal sand mining in these communities. To curb illegal sand mining, the study recommends that the current legislation on the extraction of mineral commodities in South Africa should be stringently and effectively enforced. The research further recommends that community policing forums should be established and work closely with law enforcement agencies to deal with illegal sand mining activities. Accordingly, the research study recommends the elimination of the current tedious and burdensome process of obtaining amining permit. , Thesis (MPhil) -- Faculty of Science and Agriculture, 2020
- Full Text:
- Date Issued: 2020-07
- Authors: Mbiko, Lubabalo
- Date: 2020-07
- Subjects: Ecosystem health , Biodiversity
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20474 , vital:45669
- Description: This study examined and documented the perceptions of local communities towards the socio-environmental impacts associated with illegal sand mining activities along the Umvoti River, in the KwaDukuza Local Municipality in South Africa. It was imperative to elicit the perceptions of both the local people and the government officials to understand the extent of the socio- environmental effects of illegal sand mining in the study area. To this end, a qualitative research approach was designed and used to collect the data. Data collection techniques employed included site observations and a semi-structured interview schedule with key informants. Additionally, 50 questionnaire surveys were administered to the residents of the Nsikeni and Melville communities within the study area. The results of the study revealed that illegal sand mining along the Umvoti River has contributed significantly to the degradation of grazing and farm lands, including diversion of the river and the destruction of the riparian vegetation. Evidence of water pollution, and air pollution in the form of dust, were also reported. The study observed problems in ecosystem functioning, particularly in regard to the flora and fauna, and there was extensive destruction of the aesthetic beauty of the local landscape. In addition, the study indicated that people and livestock drowned in the deep trenches that were not rehabilitated following illegal sand mining practise that was migrating along the river. Furthermore, illegal sand mining was linked with an increase in poverty and prostitution, as well as the destruction of the infrastructure in the nearby communities. Moreover, community conflicts arose from the differing perceptions on the existence of illegal sand mining in these communities. To curb illegal sand mining, the study recommends that the current legislation on the extraction of mineral commodities in South Africa should be stringently and effectively enforced. The research further recommends that community policing forums should be established and work closely with law enforcement agencies to deal with illegal sand mining activities. Accordingly, the research study recommends the elimination of the current tedious and burdensome process of obtaining amining permit. , Thesis (MPhil) -- Faculty of Science and Agriculture, 2020
- Full Text:
- Date Issued: 2020-07
- «
- ‹
- 1
- ›
- »