An evaluation of UPLC technology for the simultaneous analysis of actives in a multi-active drug
- Authors: Bawjee, Janita
- Date: 2011
- Subjects: High performance liquid chromatography
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10384 , http://hdl.handle.net/10948/d1008407 , High performance liquid chromatography
- Description: The evaluation of the potential to use Ultra Performance Liquid Chromatography (UPLC) for the simultaneous quantification of all the actives in a multi-active tablet is described in this work. Part of the evaluation was to ensure that the necessary regulatory requirements were adhered to by ascertaining that an analytical method is suitable for a specific purpose through analytical method validation for the specific multi-active tablet. The UPLC method was also tested for the analysis of similar products, namely tablet formulations that contain similar active ingredients in the same proportions but with an additional active ingredient. A method for the simultaneous determination of paracetamol, caffeine and codeine phosphate was developed using UPLC technology. The UPLC developed method was more efficient than the existing in-house HPLC method. The UPLC method was then validated in accordance to ICH and USP guidelines. The application of this UPLC method for the analysis of similar products containing paracetamol, caffeine, codeine phosphate and one extra active ingredient was very challenging. The low concentration of the additional component, differences in sample matrix and differences in formulations added to the challenges. The direct application for the analysis of products Y and Z was not successful; however the method could be used as a platform for further research. A cost comparison between the UPLC and HPLC methods showed the UPLC method to be more cost effective. Thus, while maintenance costs are higher for the UPLC instrument, column costs are comparable to HPLC columns, but solvent and waste disposal charges decrease considerably due to lower solvent use. The reduction in instrument time dramatically improves the cost effectiveness of UPLC over HPLC due to a concurrent reduction in analyst time requirement. The results of this study show that the analytical costs associated with the analysis of multi-active drugs using HPLC procedures can be reduced substantially by the CONFIDENTIAL INTELLECTUAL PROPERTY OF ASPEN PHARMACARE implementation of UPLC technology. The hypothesis that the enhanced chromatographic power of UPLC can be leveraged to provide faster analysis times hence increased product throughput rates, and lower operating costs for the analysis of multi-active drugs was accepted. These advantages were achieved whilst meeting all regulatory requirements for analytical methods as required by regulatory bodies.
- Full Text:
- Date Issued: 2011
- Authors: Bawjee, Janita
- Date: 2011
- Subjects: High performance liquid chromatography
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10384 , http://hdl.handle.net/10948/d1008407 , High performance liquid chromatography
- Description: The evaluation of the potential to use Ultra Performance Liquid Chromatography (UPLC) for the simultaneous quantification of all the actives in a multi-active tablet is described in this work. Part of the evaluation was to ensure that the necessary regulatory requirements were adhered to by ascertaining that an analytical method is suitable for a specific purpose through analytical method validation for the specific multi-active tablet. The UPLC method was also tested for the analysis of similar products, namely tablet formulations that contain similar active ingredients in the same proportions but with an additional active ingredient. A method for the simultaneous determination of paracetamol, caffeine and codeine phosphate was developed using UPLC technology. The UPLC developed method was more efficient than the existing in-house HPLC method. The UPLC method was then validated in accordance to ICH and USP guidelines. The application of this UPLC method for the analysis of similar products containing paracetamol, caffeine, codeine phosphate and one extra active ingredient was very challenging. The low concentration of the additional component, differences in sample matrix and differences in formulations added to the challenges. The direct application for the analysis of products Y and Z was not successful; however the method could be used as a platform for further research. A cost comparison between the UPLC and HPLC methods showed the UPLC method to be more cost effective. Thus, while maintenance costs are higher for the UPLC instrument, column costs are comparable to HPLC columns, but solvent and waste disposal charges decrease considerably due to lower solvent use. The reduction in instrument time dramatically improves the cost effectiveness of UPLC over HPLC due to a concurrent reduction in analyst time requirement. The results of this study show that the analytical costs associated with the analysis of multi-active drugs using HPLC procedures can be reduced substantially by the CONFIDENTIAL INTELLECTUAL PROPERTY OF ASPEN PHARMACARE implementation of UPLC technology. The hypothesis that the enhanced chromatographic power of UPLC can be leveraged to provide faster analysis times hence increased product throughput rates, and lower operating costs for the analysis of multi-active drugs was accepted. These advantages were achieved whilst meeting all regulatory requirements for analytical methods as required by regulatory bodies.
- Full Text:
- Date Issued: 2011
Representing conflict: an analysis of The Chronicle's coverage of the Gukurahundi conflict in Zimbabwe between 1983 and 1986
- Authors: Santos, Phillip
- Date: 2011
- Subjects: The Chronicle (Zimbabwe) Zimbabwe -- History -- 1980- Zimbabwe -- Politics and government -- 1980- Mass media -- Political aspects -- Zimbabwe Mass media -- Moral and ethical aspects -- Zimbabwe Journalistic ethics -- Zimbabwe Journalism -- Objectivity -- Zimbabwe War in mass media -- Zimbabwe Violence in mass media -- Zimbabwe Mass media and peace -- Zimbabwe Discourse analysis
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3481 , http://hdl.handle.net/10962/d1002936
- Description: This research is premised on the understanding that media texts are discourses and that all discourses are functional, that is, they refer to things, issues and events, in meaningful and goal oriented ways. Nine articles are analysed to explicate the sorts of discourses that were promoted by The Chronicle during the Gukurahundi conflict in Zimbabwe between 1982 and 1986. It is argued that discourses in the news media are shaped by the role(s), the type(s) of journalism assumed by such media, and by the political environment in which the news media operate. The interplay between the roles, types of journalism practised, and the effect the political environment has on news discourses is assessed within the context of conflictual situations. This is done using insights from the theoretical position of peace journalism and its critique of professional or mainstream journalism as promoting war/violence journalism. Using the case of The Chronicle's reportage of the Gukurahundi conflict in Zimbabwe, it is concluded that, in performing the collaborative role, state owned/controlled media assume characteristics of war/violence journalism. On the other hand, it is concluded that The Chronicle developed practices consistent with peace journalism when it both espoused the facilitative role and journalistic objectivity. These findings undermine the conventional view among proponents of peace journalism that in times of conflict, the news media should be interventionist in favour of peace and that they should abandon the journalistic norm of objectivity which they argue, promotes war/violence journalism.
- Full Text:
- Date Issued: 2011
- Authors: Santos, Phillip
- Date: 2011
- Subjects: The Chronicle (Zimbabwe) Zimbabwe -- History -- 1980- Zimbabwe -- Politics and government -- 1980- Mass media -- Political aspects -- Zimbabwe Mass media -- Moral and ethical aspects -- Zimbabwe Journalistic ethics -- Zimbabwe Journalism -- Objectivity -- Zimbabwe War in mass media -- Zimbabwe Violence in mass media -- Zimbabwe Mass media and peace -- Zimbabwe Discourse analysis
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3481 , http://hdl.handle.net/10962/d1002936
- Description: This research is premised on the understanding that media texts are discourses and that all discourses are functional, that is, they refer to things, issues and events, in meaningful and goal oriented ways. Nine articles are analysed to explicate the sorts of discourses that were promoted by The Chronicle during the Gukurahundi conflict in Zimbabwe between 1982 and 1986. It is argued that discourses in the news media are shaped by the role(s), the type(s) of journalism assumed by such media, and by the political environment in which the news media operate. The interplay between the roles, types of journalism practised, and the effect the political environment has on news discourses is assessed within the context of conflictual situations. This is done using insights from the theoretical position of peace journalism and its critique of professional or mainstream journalism as promoting war/violence journalism. Using the case of The Chronicle's reportage of the Gukurahundi conflict in Zimbabwe, it is concluded that, in performing the collaborative role, state owned/controlled media assume characteristics of war/violence journalism. On the other hand, it is concluded that The Chronicle developed practices consistent with peace journalism when it both espoused the facilitative role and journalistic objectivity. These findings undermine the conventional view among proponents of peace journalism that in times of conflict, the news media should be interventionist in favour of peace and that they should abandon the journalistic norm of objectivity which they argue, promotes war/violence journalism.
- Full Text:
- Date Issued: 2011
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