Exploring targeted metagenomics and untargeted metabolomics for characterising aquaponics bacterial ecology and phytochemistry
- Authors: Abraham, Benjamin Melakail
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192453 , vital:45227
- Description: Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Abraham, Benjamin Melakail
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192453 , vital:45227
- Description: Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
Design of Immunobiosensors for Detection of Tumor-Associated Anti-P53 Autoantibodies: Method Development
- Authors: Adeniyi, Omotayo Kayode
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/162988 , vital:41002 , 10.21504/10962/162988
- Description: Introduction -- Experimental -- Label-Free Impedimetric Sensing of Anti-P53ab... -- Fluorescent detection of Anti-P53ab -- Peroxidase-like activity of Fe3O4@SiNP-APTES-Au@Pd... -- Colorimetric detection of Anti-P53ab , Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020. , Detection and profiling of circulating tumor-associated autoantibodies (TAAbs) are useful for screening and early-stage diagnosis of asymptomatic lung cancer. Immunobiosensor technologies aimed to accomplish the highly sensitive, rapid and low-cost detection of TAAbs can improve the early-stage detection of lung cancer. Immunobiosensors for the detection of anti-P53-tumour associated autoantibodies have been developed in this work. The design of sensing interfaces with immobilized P53 protein (P53ag) as a sensing element layer on a solid interface was investigated. Several methods of detecting anti-P53-antibodies (anti-P53ab) were investigated. These methods are label-free detection using electrochemical impedance spectroscopy (EIS) and two label techniques. The label-free electrochemical techniques utilize gold electrode pre-modified with a conducting layer of electrochemically grafted phenylethylamine for covalent immobilization of P53ag. The limit of anti-P53ab detection with the label-free EIS was 103.0 pg.ml-1. The labeled technique developed utilizes fluorescent, and peroxidase-like nanomaterial labeled antibody as a detection probe. For the fluorescence detection, fluorescent silica nanoparticles were synthesized by overloading FITC into the silica matrix and conjugated to detection antibody (anti-IgG). The detection of the anti-P53ab was based on the dissolution of the silica nanoparticles to release the loaded dye as a signal amplification strategy. The fluorescence detection was carried out on a microplate, and magnetic bead modified P53-antigen platforms and limit of detection (LoD) were 42.0 fg.ml-1 and 3.3 fg.ml-1 for anti-P53ab; respectively. Fe3O4@SiNP-APTES-Au@Pd hybrid nanoparticles were synthesized, and their peroxidase-like activity and colorimetric detection were evaluated. The Fe3O4@SiNP-APTES-Au@Pd exhibited comparable activity to HRP. The Fe3O4@SiNP-APTES-Au@Pd was conjugated to protein-G-anti-IgG for the detection of anti-P53ab on a microplate and cellulose paper platforms. The LoD was 20.0 fg.ml-1 and 63.0 fg.ml-1 for the microplate and cellulose paper platform; respectively. The potential application of the designed immunobiosensor was evaluated in simulated serum samples. The developed sensors showed higher detection sensitivity, stability and had a lower detection limit for anti-P53ab when compared with the ELISA based detection. The results have provided alternative and effective quantification approaches to ELISA and a promising future for multiplexed detection of tumor-associated autoantibodies. The developed methodologies in this thesis could be applied for the detection of other autoantibodies in other cancer types and auto-immune diseases.
- Full Text:
- Date Issued: 2020
- Authors: Adeniyi, Omotayo Kayode
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/162988 , vital:41002 , 10.21504/10962/162988
- Description: Introduction -- Experimental -- Label-Free Impedimetric Sensing of Anti-P53ab... -- Fluorescent detection of Anti-P53ab -- Peroxidase-like activity of Fe3O4@SiNP-APTES-Au@Pd... -- Colorimetric detection of Anti-P53ab , Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020. , Detection and profiling of circulating tumor-associated autoantibodies (TAAbs) are useful for screening and early-stage diagnosis of asymptomatic lung cancer. Immunobiosensor technologies aimed to accomplish the highly sensitive, rapid and low-cost detection of TAAbs can improve the early-stage detection of lung cancer. Immunobiosensors for the detection of anti-P53-tumour associated autoantibodies have been developed in this work. The design of sensing interfaces with immobilized P53 protein (P53ag) as a sensing element layer on a solid interface was investigated. Several methods of detecting anti-P53-antibodies (anti-P53ab) were investigated. These methods are label-free detection using electrochemical impedance spectroscopy (EIS) and two label techniques. The label-free electrochemical techniques utilize gold electrode pre-modified with a conducting layer of electrochemically grafted phenylethylamine for covalent immobilization of P53ag. The limit of anti-P53ab detection with the label-free EIS was 103.0 pg.ml-1. The labeled technique developed utilizes fluorescent, and peroxidase-like nanomaterial labeled antibody as a detection probe. For the fluorescence detection, fluorescent silica nanoparticles were synthesized by overloading FITC into the silica matrix and conjugated to detection antibody (anti-IgG). The detection of the anti-P53ab was based on the dissolution of the silica nanoparticles to release the loaded dye as a signal amplification strategy. The fluorescence detection was carried out on a microplate, and magnetic bead modified P53-antigen platforms and limit of detection (LoD) were 42.0 fg.ml-1 and 3.3 fg.ml-1 for anti-P53ab; respectively. Fe3O4@SiNP-APTES-Au@Pd hybrid nanoparticles were synthesized, and their peroxidase-like activity and colorimetric detection were evaluated. The Fe3O4@SiNP-APTES-Au@Pd exhibited comparable activity to HRP. The Fe3O4@SiNP-APTES-Au@Pd was conjugated to protein-G-anti-IgG for the detection of anti-P53ab on a microplate and cellulose paper platforms. The LoD was 20.0 fg.ml-1 and 63.0 fg.ml-1 for the microplate and cellulose paper platform; respectively. The potential application of the designed immunobiosensor was evaluated in simulated serum samples. The developed sensors showed higher detection sensitivity, stability and had a lower detection limit for anti-P53ab when compared with the ELISA based detection. The results have provided alternative and effective quantification approaches to ELISA and a promising future for multiplexed detection of tumor-associated autoantibodies. The developed methodologies in this thesis could be applied for the detection of other autoantibodies in other cancer types and auto-immune diseases.
- Full Text:
- Date Issued: 2020
Design of immunosensor for the detection of C-reactive protein using oriented antibody immobilization
- Authors: Adesina, Abiola Olanike
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163080 , vital:41010 , https://dx.doi.org/10.21504/10962/163080
- Description: Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020.
- Full Text:
- Date Issued: 2020
- Authors: Adesina, Abiola Olanike
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/163080 , vital:41010 , https://dx.doi.org/10.21504/10962/163080
- Description: Thesis (PhD)--Rhodes University, Science Faculty, Department of Chemistry, 2020.
- Full Text:
- Date Issued: 2020
The design, synthesis and antiplasmodial activity of a series of halogenated fosmidomycin analogues and hybrid drugs
- Authors: Afolayan, Anthonia Folake
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64370 , vital:28538
- Description: Malaria continues to be a devastating disease and a major cause of death in sub-Saharan Africa. With resistance against most of the available antimalarial drugs, there is a need for ongoing research and development of antimalarial agents. Fosmidomycin and its acetyl analogue FR900098 have been identified as potent inhibitors of Plasmodium falciparum, the causative agent of the most deadly form of malaria. Clinical trials of these agents have revealed poor absorption due to their high hydrophilicity. In the present studies the effect of halogenation of the acyl chain as well as the biological effect of extending the acyl sidechain was explored. This provided the basis on which fosmidomycin hybrids were designed to investigate the feasibility of hybrid extending into NADPH binding pocket. Synthesis of a series of halogenated FR900098 analogues was carried out in three stages. This included i) The introduction of the phosphonate group by reaction with 1,3dibromopropane in an Arbuzov reaction, ii) The introduction of a hydroxamate group by reaction of the propyl phosphonate by means of a nucleophilic substitution reaction with BocNHOBn and iii) The introduction of a halogenated acyl side chain on a protected fosmidomycin backbone. The synthesis of fosmidomycin-hybrids for which chloroquinefosmidomycin hybrids were used as the prototype, involved convergence of the two separately constructed moieties i.e. fosmidomycin and the quinoline moieties in a covalent linkage. The quinoline moiety was easily synthesized from the reaction of 4,7dichloroquinoline with 1,2-diamino ethane. The aminoquinoline so formed resulted in chloroquine-fosmidomycin hybrids 3.8 and 3.9 when reacted with halogenated FR900098 analogues. Antiplasmodial assays were conducted on the chloroquine-fosmidomycin hybrids and the halogenated fosmidomycin derivatives against the chloroquine resistant Gambian FCR-3 strain of P. falciparum. The most potent iodoacetyl fosmidomycin analogues 2.21 gave an IC50 value of 5.54 µM which is eight times more potent than the known antiplasmodial FR900098 which gave an IC50 value of 41.67 µM. All the halogenated FR900098 analogues showed better antiplasmodial activity than their non-halogenated derivatives. This indicated that the presence of halogens in the FR900098 analogues contributes to their biological Chapter 1 Literature review activity. The acetyl and propyl linked hybrids 3.8 and 3.9 showed potent antiplasmodial activity with IC50 values of 0.18 and 0.82 µM respectively. These were by far the most potent hybrids synthesized and provided leads for a new class of promising antimalarial agents. Preliminary E. coli DXR enzyme inhibition assays were carried out on the halogenated fosmidomycin analogues. The results showed good inhibition of the enzyme by the phosphonic acids of the chloroacetyl and chloropropyl analogues 2.1 and 2.2 respectively. Molecular modelling of the compounds on E. coli (PDB code: 2EGH) and P. falciparum (PDB code: 3AUA) DXR showed strong binding of the halogenated fosmidomycin analogues while the hybrids in the absence of docked NADPH showed minimum binding to the enzymes.
- Full Text:
- Date Issued: 2012
- Authors: Afolayan, Anthonia Folake
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64370 , vital:28538
- Description: Malaria continues to be a devastating disease and a major cause of death in sub-Saharan Africa. With resistance against most of the available antimalarial drugs, there is a need for ongoing research and development of antimalarial agents. Fosmidomycin and its acetyl analogue FR900098 have been identified as potent inhibitors of Plasmodium falciparum, the causative agent of the most deadly form of malaria. Clinical trials of these agents have revealed poor absorption due to their high hydrophilicity. In the present studies the effect of halogenation of the acyl chain as well as the biological effect of extending the acyl sidechain was explored. This provided the basis on which fosmidomycin hybrids were designed to investigate the feasibility of hybrid extending into NADPH binding pocket. Synthesis of a series of halogenated FR900098 analogues was carried out in three stages. This included i) The introduction of the phosphonate group by reaction with 1,3dibromopropane in an Arbuzov reaction, ii) The introduction of a hydroxamate group by reaction of the propyl phosphonate by means of a nucleophilic substitution reaction with BocNHOBn and iii) The introduction of a halogenated acyl side chain on a protected fosmidomycin backbone. The synthesis of fosmidomycin-hybrids for which chloroquinefosmidomycin hybrids were used as the prototype, involved convergence of the two separately constructed moieties i.e. fosmidomycin and the quinoline moieties in a covalent linkage. The quinoline moiety was easily synthesized from the reaction of 4,7dichloroquinoline with 1,2-diamino ethane. The aminoquinoline so formed resulted in chloroquine-fosmidomycin hybrids 3.8 and 3.9 when reacted with halogenated FR900098 analogues. Antiplasmodial assays were conducted on the chloroquine-fosmidomycin hybrids and the halogenated fosmidomycin derivatives against the chloroquine resistant Gambian FCR-3 strain of P. falciparum. The most potent iodoacetyl fosmidomycin analogues 2.21 gave an IC50 value of 5.54 µM which is eight times more potent than the known antiplasmodial FR900098 which gave an IC50 value of 41.67 µM. All the halogenated FR900098 analogues showed better antiplasmodial activity than their non-halogenated derivatives. This indicated that the presence of halogens in the FR900098 analogues contributes to their biological Chapter 1 Literature review activity. The acetyl and propyl linked hybrids 3.8 and 3.9 showed potent antiplasmodial activity with IC50 values of 0.18 and 0.82 µM respectively. These were by far the most potent hybrids synthesized and provided leads for a new class of promising antimalarial agents. Preliminary E. coli DXR enzyme inhibition assays were carried out on the halogenated fosmidomycin analogues. The results showed good inhibition of the enzyme by the phosphonic acids of the chloroacetyl and chloropropyl analogues 2.1 and 2.2 respectively. Molecular modelling of the compounds on E. coli (PDB code: 2EGH) and P. falciparum (PDB code: 3AUA) DXR showed strong binding of the halogenated fosmidomycin analogues while the hybrids in the absence of docked NADPH showed minimum binding to the enzymes.
- Full Text:
- Date Issued: 2012
The relative suitability of knowledge paradigms to indigenous African resource management and their implications for environmental bioethics, environmental policy and food security
- Authors: Agbor Ambang, Oscar Mbi
- Date: 2020-04
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , M.Pharm
- Identifier: http://hdl.handle.net/10962/163091 , vital:41011
- Description: Thesis (M.Pharm)--Rhodes University, Faculty of Pharmacy, Pharmacy, 2020.
- Full Text:
- Date Issued: 2020-04
- Authors: Agbor Ambang, Oscar Mbi
- Date: 2020-04
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , M.Pharm
- Identifier: http://hdl.handle.net/10962/163091 , vital:41011
- Description: Thesis (M.Pharm)--Rhodes University, Faculty of Pharmacy, Pharmacy, 2020.
- Full Text:
- Date Issued: 2020-04
A phenomenological investigation of the beginning therapist's experience of the first session of psychotherapy with the first patient
- Authors: Allen, Jennifer Ann
- Date: 1987
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193129 , vital:45301
- Description: The aim of this project is to come to an understanding of how the situation of the first session of psychotherapy with the first patient is lived by the beginning therapist and what meaning this situation holds for him. The writer's interest in this phenomenon grew out of her own experience of this situation as a clinical Masters coursework student, an experience which was of important to the writer and meaningful still as a therapist in training. In dialogue with experienced therapists the importance of this situation was again made apparant. It was the opinion of these therapists that although for some the details of this experience had become dulled by time, what remained meaningful to them was that this experience was seen as the beginning of a project which remains important to them - they identified this situation as an important moment in the history of their development as psychotherapists. In the hope that the literature pertaining to psychotherapy would throw some light on this situation, the writer turned to a number of sources in this area to discover that no literature available to her elucidated this situation in a holistic manner. This led the writer to go back to the beginning therapists themselves so that they may speak for themselves of their experience of this situation. A phenomenological method of enquiry is implemented in this study as it renders the subject matter accessible to investigation, and allows it to reveal itself as it essentially is. This project is then an attempt to come to a general description of the beginning therapist's experience of the first session of psychotherapy with the first patient and thus to articulate the structure of the beginning therapist's lived situation (world) in this context. , Thesis (MA) -- Faculty of Humanities, Psychology, 1987
- Full Text:
- Date Issued: 1987
- Authors: Allen, Jennifer Ann
- Date: 1987
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193129 , vital:45301
- Description: The aim of this project is to come to an understanding of how the situation of the first session of psychotherapy with the first patient is lived by the beginning therapist and what meaning this situation holds for him. The writer's interest in this phenomenon grew out of her own experience of this situation as a clinical Masters coursework student, an experience which was of important to the writer and meaningful still as a therapist in training. In dialogue with experienced therapists the importance of this situation was again made apparant. It was the opinion of these therapists that although for some the details of this experience had become dulled by time, what remained meaningful to them was that this experience was seen as the beginning of a project which remains important to them - they identified this situation as an important moment in the history of their development as psychotherapists. In the hope that the literature pertaining to psychotherapy would throw some light on this situation, the writer turned to a number of sources in this area to discover that no literature available to her elucidated this situation in a holistic manner. This led the writer to go back to the beginning therapists themselves so that they may speak for themselves of their experience of this situation. A phenomenological method of enquiry is implemented in this study as it renders the subject matter accessible to investigation, and allows it to reveal itself as it essentially is. This project is then an attempt to come to a general description of the beginning therapist's experience of the first session of psychotherapy with the first patient and thus to articulate the structure of the beginning therapist's lived situation (world) in this context. , Thesis (MA) -- Faculty of Humanities, Psychology, 1987
- Full Text:
- Date Issued: 1987
Exploring ESL teachers’ self-developed pedagogical practices for teaching reading comprehension in Namibian primary schools
- Authors: Alumbungu, Marta Ndakalako
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419714 , vital:71669
- Description: Embargoed. Possible release date 2026 pending publication. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Alumbungu, Marta Ndakalako
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419714 , vital:71669
- Description: Embargoed. Possible release date 2026 pending publication. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2023
- Full Text:
- Date Issued: 2023-10-13
In silico analysis of plasmodium falciparum Hsp70-x for potential binding sites and hits
- Authors: Amusengeri, Arnold
- Date: 2017
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59136 , vital:27435
- Description: Restricted access-thesis embargoed for 1 year - release date April 2019
- Full Text:
- Date Issued: 2017
- Authors: Amusengeri, Arnold
- Date: 2017
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59136 , vital:27435
- Description: Restricted access-thesis embargoed for 1 year - release date April 2019
- Full Text:
- Date Issued: 2017
Self-reports on the effectiveness of psychotherapy with therapists-in-training: an 18-month follow-up study at a psychology training clinic
- Authors: Angus, Catherine Clare
- Date: 1993
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193069 , vital:45295
- Description: The purpose of this study is to ascertain whether the clients who make use of the services of the Psychology Clinic at Rhodes University perceive the masters students being trained at the Clinic as providing adequate psychotherapy. Using a sample of 18 clients, who underwent psychotherapy at the Rhodes Psychology Clinic during 1990, a follow-up study was conducted to ascertain the perceived success of that psychotherapy. Those variables most pertinent to the outcome of psychotherapy are examined. The study uses both qualitative and quantitative methodology and shows that, overall, 89% of the clients who participated in this study perceived the psychotherapy at the Rhodes Psychology Clinic to be satisfactory. The study also highlights those factors which were perceived to be negative in the psychotherapeutic equation and it is hoped that the findings can be beneficial in planning more efficient services for the Clinic in the future. , Thesis (MA) -- Faculty of Humanities, Psychology, 1993
- Full Text:
- Date Issued: 1993
- Authors: Angus, Catherine Clare
- Date: 1993
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193069 , vital:45295
- Description: The purpose of this study is to ascertain whether the clients who make use of the services of the Psychology Clinic at Rhodes University perceive the masters students being trained at the Clinic as providing adequate psychotherapy. Using a sample of 18 clients, who underwent psychotherapy at the Rhodes Psychology Clinic during 1990, a follow-up study was conducted to ascertain the perceived success of that psychotherapy. Those variables most pertinent to the outcome of psychotherapy are examined. The study uses both qualitative and quantitative methodology and shows that, overall, 89% of the clients who participated in this study perceived the psychotherapy at the Rhodes Psychology Clinic to be satisfactory. The study also highlights those factors which were perceived to be negative in the psychotherapeutic equation and it is hoped that the findings can be beneficial in planning more efficient services for the Clinic in the future. , Thesis (MA) -- Faculty of Humanities, Psychology, 1993
- Full Text:
- Date Issued: 1993
Composition Portfolio
- Authors: Appollis, Sylvester
- Date: 201u
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193476 , vital:45335
- Description: Composition portfolio. , Thesis (MMus) -- Faculty of Humanities, Music and Musicology, 201u
- Full Text:
- Date Issued: 201u
- Authors: Appollis, Sylvester
- Date: 201u
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193476 , vital:45335
- Description: Composition portfolio. , Thesis (MMus) -- Faculty of Humanities, Music and Musicology, 201u
- Full Text:
- Date Issued: 201u
Archontic Aporias: the mediums of memory
- Authors: Arbuckle, Julia Ruth
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408727 , vital:70520
- Description: Contextualising my research within the Eastern Cape as a descendant of 1820 settlers, I question the modalities of historical recollection by introducing memory as a viable mode of archival production alongside that of the archive. Through interrogating Eastern Cape archival institutes and employing an autoethnographic approach to my familial archives, I show that archival curation affects the gaps, schisms, and interpretations of archives as much as the ‘unreliability’ of memory. I rely on definitions from Jacques Derrida and literature from Achille Mbembe and Verne Harris, as well as reflexive methodologies, to engage the ways of remembering the past and methods of storytelling. With this undertaking, I expose the aporias within archival processes. This written component is part of broader research that encompasses theoretical study and a practice-based Fine Arts research project culminating in an exhibition that shares themes of memory, archive, trauma, and curatorial and personal heritage management. This research engages in case studies of artworks by Angela Deane and Maureen de Jager to contextualise and position the creative process. , Thesis (MFA) -- Faculty of Humanities, Fine Art, 2023
- Full Text:
- Date Issued: 2023-03-30
- Authors: Arbuckle, Julia Ruth
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408727 , vital:70520
- Description: Contextualising my research within the Eastern Cape as a descendant of 1820 settlers, I question the modalities of historical recollection by introducing memory as a viable mode of archival production alongside that of the archive. Through interrogating Eastern Cape archival institutes and employing an autoethnographic approach to my familial archives, I show that archival curation affects the gaps, schisms, and interpretations of archives as much as the ‘unreliability’ of memory. I rely on definitions from Jacques Derrida and literature from Achille Mbembe and Verne Harris, as well as reflexive methodologies, to engage the ways of remembering the past and methods of storytelling. With this undertaking, I expose the aporias within archival processes. This written component is part of broader research that encompasses theoretical study and a practice-based Fine Arts research project culminating in an exhibition that shares themes of memory, archive, trauma, and curatorial and personal heritage management. This research engages in case studies of artworks by Angela Deane and Maureen de Jager to contextualise and position the creative process. , Thesis (MFA) -- Faculty of Humanities, Fine Art, 2023
- Full Text:
- Date Issued: 2023-03-30
South African traditional healers’ perceptions and treatment of the symptoms of depression
- Authors: Augustine, Daniella Joan
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408789 , vital:70525
- Description: While depression is experienced worldwide, the World Health Organization indicated that over 80% of this disease burden is found in low- and middle-income countries, with 16% of depressive disorder cases being found in Africa. Specifically, in South Africa, the lifetime prevalence of major depressive disorder is 9.7%. Research conducted on traditional healing estimates that as high as 80% of black individuals in South Africa choose to consult traditional healers. Although there was initial consensus that the use of traditional healers reflected a lack of access to alternative resources, more recent research has however indicated that traditional healers are consulted even when psychiatric facilities and medication are available. Research has illustrated that many patients that chose to consult traditional healers do so as a result of their shared beliefs, and traditional healers' ability to understand the cultural frameworks underlying illness. The current study investigated how traditional healers in the Eastern Cape perceive and treat phenomena currently understood as the symptoms of depression and how these understandings can be incorporated into collaborations between Western medicine and traditional healing practice. A qualitative research design was used to examine the perspectives and treatment methods of isiXhosa traditional healers in the Eastern Cape. Four healers participated in the study. Key findings indicated that there is no single cause or definition of the phenomenon of depression by healers. Causality is greatly attributed to spiritual causes and ancestors play a key role in guiding the diagnosis and multiple treatment interventions employed by healers to treat the experience of depression. Finally, all traditional healers expressed a willingness to collaborate and work with the Western healthcare system, however, they indicated a strong need for greater recognition as a valid healthcare system. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-03-30
- Authors: Augustine, Daniella Joan
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408789 , vital:70525
- Description: While depression is experienced worldwide, the World Health Organization indicated that over 80% of this disease burden is found in low- and middle-income countries, with 16% of depressive disorder cases being found in Africa. Specifically, in South Africa, the lifetime prevalence of major depressive disorder is 9.7%. Research conducted on traditional healing estimates that as high as 80% of black individuals in South Africa choose to consult traditional healers. Although there was initial consensus that the use of traditional healers reflected a lack of access to alternative resources, more recent research has however indicated that traditional healers are consulted even when psychiatric facilities and medication are available. Research has illustrated that many patients that chose to consult traditional healers do so as a result of their shared beliefs, and traditional healers' ability to understand the cultural frameworks underlying illness. The current study investigated how traditional healers in the Eastern Cape perceive and treat phenomena currently understood as the symptoms of depression and how these understandings can be incorporated into collaborations between Western medicine and traditional healing practice. A qualitative research design was used to examine the perspectives and treatment methods of isiXhosa traditional healers in the Eastern Cape. Four healers participated in the study. Key findings indicated that there is no single cause or definition of the phenomenon of depression by healers. Causality is greatly attributed to spiritual causes and ancestors play a key role in guiding the diagnosis and multiple treatment interventions employed by healers to treat the experience of depression. Finally, all traditional healers expressed a willingness to collaborate and work with the Western healthcare system, however, they indicated a strong need for greater recognition as a valid healthcare system. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-03-30
An in vitro investigation of novel quinolone derivatives on selected pharmacological targets for diabetes mellitus and associated complications
- Authors: Ayodele, Omobolanle Opeyemi
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/409813 , vital:70632
- Description: Diabetes mellitus (DM) is a group of endocrine and metabolic disorders characterised and identified by the presence of hyperglycaemia over a long period and, to an extent, accompanied by hyperlipidaemia. CVD has been reported to be the leading cause of mortality in patients with DM. Several antidiabetic agents are available for managing DM, but these agents are not for curative therapy and present with undesirable side effects. In addition, these agents become less effective as the patient's condition progresses to complete beta-cell failure. Therefore, developing newer antidiabetic agents with minimal undesirable side effects, prolonged efficacy and protection against the development of DM complications are necessary. This study was conducted to identify potential novel antidiabetic agents with cardiovascular-protective activity. The compounds of interest for the study were quinolone derivatives since quinolones have been reported to have an antihyperglycaemic effect. , Thesis (MSc) -- Faculty of Pharmacy, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Ayodele, Omobolanle Opeyemi
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/409813 , vital:70632
- Description: Diabetes mellitus (DM) is a group of endocrine and metabolic disorders characterised and identified by the presence of hyperglycaemia over a long period and, to an extent, accompanied by hyperlipidaemia. CVD has been reported to be the leading cause of mortality in patients with DM. Several antidiabetic agents are available for managing DM, but these agents are not for curative therapy and present with undesirable side effects. In addition, these agents become less effective as the patient's condition progresses to complete beta-cell failure. Therefore, developing newer antidiabetic agents with minimal undesirable side effects, prolonged efficacy and protection against the development of DM complications are necessary. This study was conducted to identify potential novel antidiabetic agents with cardiovascular-protective activity. The compounds of interest for the study were quinolone derivatives since quinolones have been reported to have an antihyperglycaemic effect. , Thesis (MSc) -- Faculty of Pharmacy, 2023
- Full Text:
- Date Issued: 2023-03-29
Potential of the Gravel Filter Towers, Pilot-scale Filter system and Biochar/Clay adsorbents for Bio-Physicochemical remediation and Desalination of greywater
- Authors: Bani, Siphumze
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/188375 , vital:44748
- Description: Thesis embargoed until 2023 , Thesis (MSc) -- Faculty of Science, Rhodes University Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Bani, Siphumze
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/188375 , vital:44748
- Description: Thesis embargoed until 2023 , Thesis (MSc) -- Faculty of Science, Rhodes University Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
"Burnout" in children's home houseparents
- Authors: Bath, Peter John
- Date: 1983
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193118 , vital:45300
- Description: Aimed at replicating the results of an American study into "Burnout" in Group Home houseparents, this research had the following aims: 1) To ascertain the validity and reliability of the instrument used in the original study and presented as a "useful measure of burnout”. 2) To establish whether this line of research, within a highly problematic research field, can at present offer any guidelines in the resolution of the current staffing crisis faced by South African children's homes. 3} To describe more closely the burnout syndrome. 4) To study possible etiological factors within a local context. Sixty three houseparents completed questionaires and three independent measures of burnout were obtained. The results were regarded as having failed to replicate those of the original study. The reason for this was found to be the low validity of the original instrument. The main conclusion drawn was that the line of research adopted in the original study can offer only very tentative guidelines towards the resolution of the staffing crisis faced until such time as valid and reliable instruments to measure burnout have been developed. Many of the suggested relationships between situational variables and ones of personal characteristics were confirmed for the local population of houseparents. , Thesis (MSocSci) -- Faculty of Humanities, Psychology, 1983
- Full Text:
- Date Issued: 1983
- Authors: Bath, Peter John
- Date: 1983
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193118 , vital:45300
- Description: Aimed at replicating the results of an American study into "Burnout" in Group Home houseparents, this research had the following aims: 1) To ascertain the validity and reliability of the instrument used in the original study and presented as a "useful measure of burnout”. 2) To establish whether this line of research, within a highly problematic research field, can at present offer any guidelines in the resolution of the current staffing crisis faced by South African children's homes. 3} To describe more closely the burnout syndrome. 4) To study possible etiological factors within a local context. Sixty three houseparents completed questionaires and three independent measures of burnout were obtained. The results were regarded as having failed to replicate those of the original study. The reason for this was found to be the low validity of the original instrument. The main conclusion drawn was that the line of research adopted in the original study can offer only very tentative guidelines towards the resolution of the staffing crisis faced until such time as valid and reliable instruments to measure burnout have been developed. Many of the suggested relationships between situational variables and ones of personal characteristics were confirmed for the local population of houseparents. , Thesis (MSocSci) -- Faculty of Humanities, Psychology, 1983
- Full Text:
- Date Issued: 1983
Biochemical characterisation and small molecule modulation of the interaction between two cytosolic Hsp70s from Trypanosoma brucei and potential co-chaperones
- Authors: Bentley, Stephen John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63402 , vital:28407
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
- Authors: Bentley, Stephen John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63402 , vital:28407
- Description: Expected release date-April 2019
- Full Text:
- Date Issued: 2018
A study of four-dimensional oscillator groups and the associated left-invariant control affine systems
- Authors: Biggs, Rory
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164941 , vital:41186
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Department of Mathematics, 2012
- Full Text:
- Date Issued: 2012
- Authors: Biggs, Rory
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164941 , vital:41186
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Department of Mathematics, 2012
- Full Text:
- Date Issued: 2012
Constructions of Ukuyalwa and marriage satisfaction: experiences of Xhosa couples in the Eastern Cape
- Authors: Bikwe, Siphuxolo
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408805 , vital:70527
- Description: Marital satisfaction may be described as the extent to which one’s expectations, desires and needs are being satisfied in their marriage. Studies that concern marital satisfaction provide us with crucial insights into factors that support or compromise the longevity of long-term, committed relationships. Little work has been done in understanding the role of cultural practices in marital satisfaction particularly in Africa, and South Africa more specifically. Ukuyalwa is a Xhosa ritual that takes place during a Xhosa traditional wedding, whereby the bride is introduced to the (traditional) guiding practices of her new home by the women of the family together with her sisters-in-law. This study informs the practice of African psychology as formulated by Ratele (2017b).Critical African psychology as a framework recognizes the construction of culture alongside political, socioeconomic, and historical matrices. These are acknowledged as an important element in psychological theories, methods, and explanations. This research project aimed to identify the discourses that circulate around ukuyalwa as a Xhosa cultural practice and consider the implications for the marital satisfaction of amaXhosa couples. The study interviewed 3 Xhosa couples of varying ages who had been married for different durations to develop an understanding of their experiences of ukuyalwa and marital satisfaction. Data was analyzed using a Foucauldian Discourse Analysis (FDA) which allowed for the identifications of the discourses that the couples used to construct ukuyalwa and marital satisfaction. The couples discursively constructed marriage as ‘divine unity’, ‘unification of families’ and ‘partnership’. Ukuyalwa was constructed as a ‘gendered process’, ideas of tradition and modernity, and a ‘foundation’ also became apparent. Satisfaction was constructed as ‘open communication’ and ‘work’. The couples’ positions shifted in their conversations, where in some instances they were 'learners’, ‘initiates’, and/or ‘passive’, and in other instances, they were ‘in charge’, ‘knowledgeable’, and ‘experts’. The discourses of culture, religion, and partnership circulated prominently in relation to marriage, ukuyalwa, and marital satisfaction. The findings of the study speak to how the couples’ constructions of marriage and cultural customs such as ukuyalwa are valuable as they often inform how couples behave in their marriage relationships and what they regard as satisfactory to them when it comes to marriage. These insights into what informs the couples’ ideas on marriage and satisfaction can be utilised in informing couples therapy interventions. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-03-30
- Authors: Bikwe, Siphuxolo
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408805 , vital:70527
- Description: Marital satisfaction may be described as the extent to which one’s expectations, desires and needs are being satisfied in their marriage. Studies that concern marital satisfaction provide us with crucial insights into factors that support or compromise the longevity of long-term, committed relationships. Little work has been done in understanding the role of cultural practices in marital satisfaction particularly in Africa, and South Africa more specifically. Ukuyalwa is a Xhosa ritual that takes place during a Xhosa traditional wedding, whereby the bride is introduced to the (traditional) guiding practices of her new home by the women of the family together with her sisters-in-law. This study informs the practice of African psychology as formulated by Ratele (2017b).Critical African psychology as a framework recognizes the construction of culture alongside political, socioeconomic, and historical matrices. These are acknowledged as an important element in psychological theories, methods, and explanations. This research project aimed to identify the discourses that circulate around ukuyalwa as a Xhosa cultural practice and consider the implications for the marital satisfaction of amaXhosa couples. The study interviewed 3 Xhosa couples of varying ages who had been married for different durations to develop an understanding of their experiences of ukuyalwa and marital satisfaction. Data was analyzed using a Foucauldian Discourse Analysis (FDA) which allowed for the identifications of the discourses that the couples used to construct ukuyalwa and marital satisfaction. The couples discursively constructed marriage as ‘divine unity’, ‘unification of families’ and ‘partnership’. Ukuyalwa was constructed as a ‘gendered process’, ideas of tradition and modernity, and a ‘foundation’ also became apparent. Satisfaction was constructed as ‘open communication’ and ‘work’. The couples’ positions shifted in their conversations, where in some instances they were 'learners’, ‘initiates’, and/or ‘passive’, and in other instances, they were ‘in charge’, ‘knowledgeable’, and ‘experts’. The discourses of culture, religion, and partnership circulated prominently in relation to marriage, ukuyalwa, and marital satisfaction. The findings of the study speak to how the couples’ constructions of marriage and cultural customs such as ukuyalwa are valuable as they often inform how couples behave in their marriage relationships and what they regard as satisfactory to them when it comes to marriage. These insights into what informs the couples’ ideas on marriage and satisfaction can be utilised in informing couples therapy interventions. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-03-30
Neutral Atomic Hydrogen in Gravitationally Lensed Systems
- Authors: Blecher, Tariq Dylan
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192776 , vital:45263
- Description: Thesis (PhD) -- Faculty of Law, Law, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Blecher, Tariq Dylan
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192776 , vital:45263
- Description: Thesis (PhD) -- Faculty of Law, Law, 2021
- Full Text:
- Date Issued: 2021-10-29
Diversified computational approaches for the identification of orthosteric drugs, allosteric modulators and unveiling drug resistance mechanisms: application to infectious diseases
- Authors: Boateng, Rita Afriyie
- Date: 2022-04-08
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/234173 , vital:50169
- Description: Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-04-08
- Authors: Boateng, Rita Afriyie
- Date: 2022-04-08
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/234173 , vital:50169
- Description: Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2022
- Full Text:
- Date Issued: 2022-04-08