Assessing the physical fitness level of children with intellectual disability in the Grahamstown region of the Eastern Cape, and subsequently designing, implementing and evaluating the efficacy of an exercise intervention
- Authors: Parsons, Samantha Lee
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/19723 , vital:22478
- Description: Background: Extensive research has addressed physical fitness as an agent in promoting health and well-being; however, there is little research on this topic for special populations, such as individuals with intellectual disability and even less relating to the South African context. Children with intellectual disability attending special need schools in disadvantaged communities in the Eastern Cape have lacked the opportunity to participate in structured physical education programs. Implementing a solution to this problem was seen to be a challenge, due to the lack of informative research and available data. Aims: The purpose of this research was two-fold; firstly, it aimed to identify the physical fitness levels of the intellectually disabled children in the Grahamstown region of the Eastern Cape province of South Africa; and secondly, it aimed to implement and evaluate the efficacy of an exercise intervention programme on physical fitness in a local school for children with intellectual disability. Phase 1: The descriptive study compared basic anthropometric as well as physical fitness measures from a sample of participants with intellectual disability (n=29) with a comparable sample of typically developed children (n=25). There were 15 males and 14 females in the intellectually disabled sample group, with an average age of 10.69 ± 1.26 years, and the typically developed sample had 13 males and 12 females, with an average age of 10.51 ± 0.74 years. The anthropometric measures included stature and mass; while the health-related physical fitness components included cardiorespiratory endurance, muscular endurance, strength, body composition, and flexibility; and the skill-related physical fitness components were balance, agility, speed, power, coordination and reaction time. The results revealed that the children with intellectual disability were significantly shorter in stature and lighter in mass (p <0.001) compared to their typically developed peers. Muscular endurance and strength, balance, speed, power, coordination and reaction time were poorer among those with intellectual disabilities (p <0.05). Cardiorespiratory endurance, flexibility and agility were similar between groups. Results of the correlation analyses determined significant (p <0.05) relationships between certain health-related and/or skill-related physical fitness components, which were instrumental for selecting measures for Phase 2. The physical fitness components that had the most significant (p <0.05) correlations were speed with five significant correlations, both muscular endurance and balance with six, coordination with seven, and power with eight significant correlations of the eleven physical fitness components. The comparative results, in conjunction with the correlations, determined that the most suitable physical fitness components to be implemented in the exercise intervention for Phase 2 were muscular endurance, balance, and power. Phase 2: The intervention study was a case-control study (intervention group: n = 16; control group: n = 15), whereby the intervention group was exposed to an 8-week multi-modal exercise intervention training muscular endurance, balance and power. Pre-and post-intervention measures were performed using the same eleven physical fitness tests as in Phase 1. The interaction effects and effect sizes were determined and the results showed significant improvements and large effect sizes for the intervention group’s performance of muscular endurance (p=0.026; d=0.617) and power (p<0.000; d=0.999), whereas no significant changes were found for balance. Furthermore, the exercise intervention also impacted the overall level of physical fitness, as significant changes were found for cardiorespiratory endurance (p<0.001; d=0.98), strength (p=0.021; d=0.654) and flexibility (p=0.032; d=586). In conclusion, the exercise intervention was effective for improving the intellectually disabled participants’ muscular endurance and power but not found effective for balance.
- Full Text:
- Date Issued: 2017
- Authors: Parsons, Samantha Lee
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/19723 , vital:22478
- Description: Background: Extensive research has addressed physical fitness as an agent in promoting health and well-being; however, there is little research on this topic for special populations, such as individuals with intellectual disability and even less relating to the South African context. Children with intellectual disability attending special need schools in disadvantaged communities in the Eastern Cape have lacked the opportunity to participate in structured physical education programs. Implementing a solution to this problem was seen to be a challenge, due to the lack of informative research and available data. Aims: The purpose of this research was two-fold; firstly, it aimed to identify the physical fitness levels of the intellectually disabled children in the Grahamstown region of the Eastern Cape province of South Africa; and secondly, it aimed to implement and evaluate the efficacy of an exercise intervention programme on physical fitness in a local school for children with intellectual disability. Phase 1: The descriptive study compared basic anthropometric as well as physical fitness measures from a sample of participants with intellectual disability (n=29) with a comparable sample of typically developed children (n=25). There were 15 males and 14 females in the intellectually disabled sample group, with an average age of 10.69 ± 1.26 years, and the typically developed sample had 13 males and 12 females, with an average age of 10.51 ± 0.74 years. The anthropometric measures included stature and mass; while the health-related physical fitness components included cardiorespiratory endurance, muscular endurance, strength, body composition, and flexibility; and the skill-related physical fitness components were balance, agility, speed, power, coordination and reaction time. The results revealed that the children with intellectual disability were significantly shorter in stature and lighter in mass (p <0.001) compared to their typically developed peers. Muscular endurance and strength, balance, speed, power, coordination and reaction time were poorer among those with intellectual disabilities (p <0.05). Cardiorespiratory endurance, flexibility and agility were similar between groups. Results of the correlation analyses determined significant (p <0.05) relationships between certain health-related and/or skill-related physical fitness components, which were instrumental for selecting measures for Phase 2. The physical fitness components that had the most significant (p <0.05) correlations were speed with five significant correlations, both muscular endurance and balance with six, coordination with seven, and power with eight significant correlations of the eleven physical fitness components. The comparative results, in conjunction with the correlations, determined that the most suitable physical fitness components to be implemented in the exercise intervention for Phase 2 were muscular endurance, balance, and power. Phase 2: The intervention study was a case-control study (intervention group: n = 16; control group: n = 15), whereby the intervention group was exposed to an 8-week multi-modal exercise intervention training muscular endurance, balance and power. Pre-and post-intervention measures were performed using the same eleven physical fitness tests as in Phase 1. The interaction effects and effect sizes were determined and the results showed significant improvements and large effect sizes for the intervention group’s performance of muscular endurance (p=0.026; d=0.617) and power (p<0.000; d=0.999), whereas no significant changes were found for balance. Furthermore, the exercise intervention also impacted the overall level of physical fitness, as significant changes were found for cardiorespiratory endurance (p<0.001; d=0.98), strength (p=0.021; d=0.654) and flexibility (p=0.032; d=586). In conclusion, the exercise intervention was effective for improving the intellectually disabled participants’ muscular endurance and power but not found effective for balance.
- Full Text:
- Date Issued: 2017
Assessing the potential impact of climate change in the south-eastern Great Escarpment, Southern Africa
- Authors: Bentley, Luke Kinross
- Date: 2017
- Subjects: Mountain plants -- Climatic factors -- Drakensberg Mountains , Mountain biodiversity -- Drakensberg Mountains , Climatic changes -- Drakensberg Mountains , Rain and rainfall -- Drakensberg Mountains , Plant diversity -- Climatic factors -- Drakensberg Mountains
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4657 , vital:20709
- Description: Rapid, anthropogenic climate change is a contemporary phenomenon which is threatening natural ecosystems. Mountains are globally important, housing high levels of plant and animal biodiversity, as well as being important suppliers of water with 50% of mountainous regions being essential for the provision of water to downstream inhabitants. Owing to their importance, an assessment of the historical, current and potential impacts of climate change on the south-eastern Great Escarpment encompassing South Africa and Lesotho is necessary and is the primary aim of this thesis. In order to assess the historical and current impacts of climate change, historical rainfall records were collected from 78 sites throughout the Eastern Cape. These records were used to determine rainfall trends and trends in the frequency of daily extreme events. Climate change projections predict increasing trends in summer and annual rainfall, and a marginal increase to a slightly decreasing trend in winter rainfall, depending on if statistically downscaled or dynamically downscaled projections are examined. Extreme events are also predicted to increase in frequency. Results showed trends which generally agree with the predictions, except for extreme events for which there was insufficient data to support the projections. This has implications for farmers in the area and validates the downscaled Regional Climate Models (RCMs) to be used for the predictive analysis determining the potential future impacts of climate change on plant species' distributions in the south-eastern Great Escarpment. In order to assess montane plant species to future climate predictions, occurrence records of 46 species were correlated to rainfall and temperature predictor variables and predictions for species richness and beta diversity were made for current and future climates. This predictive analysis used and compared two correlative species distribution modelling methods - one method used one correlating algorithm in a nuanced manner and the other method used five correlating algorithms in an ensemble. A trend of range restriction to higher elevations for the 46 species analysed was as expected from a warming climate. The nuanced, single algorithm modelling method produced less conservative models. The predicted trend of an elevational increase in montane vegetation in this region points to Lesotho and the Drakensberg highlands as being an important refugium for montane plant taxa of the southeastern Great Escarpment. The Maloti Drakensberg Transfrontier Conservation Area hence needs to be expanded and its mandate properly enforced to protect this region adequately.
- Full Text:
- Date Issued: 2017
- Authors: Bentley, Luke Kinross
- Date: 2017
- Subjects: Mountain plants -- Climatic factors -- Drakensberg Mountains , Mountain biodiversity -- Drakensberg Mountains , Climatic changes -- Drakensberg Mountains , Rain and rainfall -- Drakensberg Mountains , Plant diversity -- Climatic factors -- Drakensberg Mountains
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4657 , vital:20709
- Description: Rapid, anthropogenic climate change is a contemporary phenomenon which is threatening natural ecosystems. Mountains are globally important, housing high levels of plant and animal biodiversity, as well as being important suppliers of water with 50% of mountainous regions being essential for the provision of water to downstream inhabitants. Owing to their importance, an assessment of the historical, current and potential impacts of climate change on the south-eastern Great Escarpment encompassing South Africa and Lesotho is necessary and is the primary aim of this thesis. In order to assess the historical and current impacts of climate change, historical rainfall records were collected from 78 sites throughout the Eastern Cape. These records were used to determine rainfall trends and trends in the frequency of daily extreme events. Climate change projections predict increasing trends in summer and annual rainfall, and a marginal increase to a slightly decreasing trend in winter rainfall, depending on if statistically downscaled or dynamically downscaled projections are examined. Extreme events are also predicted to increase in frequency. Results showed trends which generally agree with the predictions, except for extreme events for which there was insufficient data to support the projections. This has implications for farmers in the area and validates the downscaled Regional Climate Models (RCMs) to be used for the predictive analysis determining the potential future impacts of climate change on plant species' distributions in the south-eastern Great Escarpment. In order to assess montane plant species to future climate predictions, occurrence records of 46 species were correlated to rainfall and temperature predictor variables and predictions for species richness and beta diversity were made for current and future climates. This predictive analysis used and compared two correlative species distribution modelling methods - one method used one correlating algorithm in a nuanced manner and the other method used five correlating algorithms in an ensemble. A trend of range restriction to higher elevations for the 46 species analysed was as expected from a warming climate. The nuanced, single algorithm modelling method produced less conservative models. The predicted trend of an elevational increase in montane vegetation in this region points to Lesotho and the Drakensberg highlands as being an important refugium for montane plant taxa of the southeastern Great Escarpment. The Maloti Drakensberg Transfrontier Conservation Area hence needs to be expanded and its mandate properly enforced to protect this region adequately.
- Full Text:
- Date Issued: 2017
Assessing the potential role of microorganisms in the production of seedlings for the restoration of Albany Thicket
- Authors: Mpama, Nelisa
- Date: 2017
- Subjects: Vesicular-arbuscular mycorrhizas , Rhizobacteria , Restoration ecology South Africa Albany , Microorganisms
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64555 , vital:28558
- Description: The role of microorganisms in restoration of the Albany Thicket has not been well documented, although the benefits to plants of these various interactions has been well documented. Microorganisms are chief ecological engineers and assist in resolving environmental problems and act to restore degraded ecosystem function by forming mutual relationships with the roots of the plants. The aim of this study was to assess the potential of microorganisms for the improved biomass production of selected woody and succulent seedlings used in mesic thicket restoration. Three tree species were selected for propagation in this study namely; Mystroxylon aethiopicum Scutia myrtina and Aloe ferox. Soil samples were collected from a degraded and intact thicket site from Bathurst, South Africa. Soils were evaluated for number of arbuscular mycorrhizal (AM) spores, mycorrhizal infectivity potential and nutrient availability both before and after seedling propagation. Pasteurized soil from the degraded site was used in a pot trial. Ten replicates seedling for plant species were planted and subjected to four treatments which included inoculation with AM fungi and the rhizobacterium, Enterobacter sp., alone and in combination; the fourth treatment was an un-inouclated control. Plant growth parameters were recorded at regular intervals where appropriate and seedlings were harvested after 24 weeks for biomass measurements and AM colonisation assessments. Although generally low (< 1 spore per gram) the density of AM fungal spores was significantly higher in soils from the intact site when compared with soils from the degraded site. The mycorrhizal potential of the soils was however not significantly different. Mystroxylon aethiopicum seedling shoot height, canopy diameter and shoot biomass showed a significant increase when inoculated with AM fungi while S. myrtina seedlings showed increased shoot height when inoculated with both AM fungi and Enterobacter sp. Aloe ferox seedlings did not respond to microbial inoculation. The concentration of soil P and Na increased in treatments with Enterobacter sp. alone and in combination with AM fungi. Mystroxylon aethiopicum and S. myrtina seedlings showed a dependency on microbial inoculants indicating the importance of inoculation in the nursery before planting out into the field. Overall AM fungal inoculants applied to seedlings can be used to compensate for nutrient deficiency in soils. Although the Enterobacter isolate used was known to have various plant growth promoting capabilities. It is recommended that other rhizobacterial isolates be investigated. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2017
- Full Text:
- Date Issued: 2017
- Authors: Mpama, Nelisa
- Date: 2017
- Subjects: Vesicular-arbuscular mycorrhizas , Rhizobacteria , Restoration ecology South Africa Albany , Microorganisms
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/64555 , vital:28558
- Description: The role of microorganisms in restoration of the Albany Thicket has not been well documented, although the benefits to plants of these various interactions has been well documented. Microorganisms are chief ecological engineers and assist in resolving environmental problems and act to restore degraded ecosystem function by forming mutual relationships with the roots of the plants. The aim of this study was to assess the potential of microorganisms for the improved biomass production of selected woody and succulent seedlings used in mesic thicket restoration. Three tree species were selected for propagation in this study namely; Mystroxylon aethiopicum Scutia myrtina and Aloe ferox. Soil samples were collected from a degraded and intact thicket site from Bathurst, South Africa. Soils were evaluated for number of arbuscular mycorrhizal (AM) spores, mycorrhizal infectivity potential and nutrient availability both before and after seedling propagation. Pasteurized soil from the degraded site was used in a pot trial. Ten replicates seedling for plant species were planted and subjected to four treatments which included inoculation with AM fungi and the rhizobacterium, Enterobacter sp., alone and in combination; the fourth treatment was an un-inouclated control. Plant growth parameters were recorded at regular intervals where appropriate and seedlings were harvested after 24 weeks for biomass measurements and AM colonisation assessments. Although generally low (< 1 spore per gram) the density of AM fungal spores was significantly higher in soils from the intact site when compared with soils from the degraded site. The mycorrhizal potential of the soils was however not significantly different. Mystroxylon aethiopicum seedling shoot height, canopy diameter and shoot biomass showed a significant increase when inoculated with AM fungi while S. myrtina seedlings showed increased shoot height when inoculated with both AM fungi and Enterobacter sp. Aloe ferox seedlings did not respond to microbial inoculation. The concentration of soil P and Na increased in treatments with Enterobacter sp. alone and in combination with AM fungi. Mystroxylon aethiopicum and S. myrtina seedlings showed a dependency on microbial inoculants indicating the importance of inoculation in the nursery before planting out into the field. Overall AM fungal inoculants applied to seedlings can be used to compensate for nutrient deficiency in soils. Although the Enterobacter isolate used was known to have various plant growth promoting capabilities. It is recommended that other rhizobacterial isolates be investigated. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2017
- Full Text:
- Date Issued: 2017
Automation of source-artefact classification
- Sebokolodi, Makhuduga Lerato Lydia
- Authors: Sebokolodi, Makhuduga Lerato Lydia
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4920 , vital:20743
- Description: The high sensitivities of modern radio telescopes will enable the detection of very faint astrophysical sources in the distant Universe. However, these high sensitivities also imply that calibration artefacts, which were below the noise for less sensitive instruments, will emerge above the noise and may limit the dynamic range capabilities of these instruments. Detecting faint emission will require detection thresholds close to the noise and this may cause some of the artefacts to be incorrectly detected as real emission. The current approach is to manually remove the artefacts, or set high detection thresholds in order to avoid them. The former will not be possible given the large quantities of data that these instruments will produce, and the latter results in very shallow and incomplete catalogues. This work uses the negative detection method developed by Serra et al. (2012) to distinguish artefacts from astrophysical emission in radio images. We also present a technique that automates the identification of sources subject to severe direction-dependent (DD) effects and thus allows them to be flagged for DD calibration. The negative detection approach is shown to provide high reliability and high completeness catalogues for simulated data, as well as a JVLA observation of the 3C147 field (Mitra et al., 2015). We also show that our technique correctly identifies sources that require DD calibration for datasets from the KAT-7, LOFAR, JVLA and GMRT instruments.
- Full Text:
- Date Issued: 2017
- Authors: Sebokolodi, Makhuduga Lerato Lydia
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4920 , vital:20743
- Description: The high sensitivities of modern radio telescopes will enable the detection of very faint astrophysical sources in the distant Universe. However, these high sensitivities also imply that calibration artefacts, which were below the noise for less sensitive instruments, will emerge above the noise and may limit the dynamic range capabilities of these instruments. Detecting faint emission will require detection thresholds close to the noise and this may cause some of the artefacts to be incorrectly detected as real emission. The current approach is to manually remove the artefacts, or set high detection thresholds in order to avoid them. The former will not be possible given the large quantities of data that these instruments will produce, and the latter results in very shallow and incomplete catalogues. This work uses the negative detection method developed by Serra et al. (2012) to distinguish artefacts from astrophysical emission in radio images. We also present a technique that automates the identification of sources subject to severe direction-dependent (DD) effects and thus allows them to be flagged for DD calibration. The negative detection approach is shown to provide high reliability and high completeness catalogues for simulated data, as well as a JVLA observation of the 3C147 field (Mitra et al., 2015). We also show that our technique correctly identifies sources that require DD calibration for datasets from the KAT-7, LOFAR, JVLA and GMRT instruments.
- Full Text:
- Date Issued: 2017
Back to nowhere
- Authors: Fundakubi, Zukile Anthony
- Date: 2017
- Subjects: Creative writing (Higher education) South Africa , South African fiction (English) 21st century , Short stories, South African (English) 21st century , Xhosa fiction 21st century , Short stories, Xhosa 21st century , Detective and mystery stories 21st century
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/5071 , vital:20763
- Description: My writing contains elements of hard-boiled detective fiction and crime writing. My stories, written in isiXhosa and English and a mixture of both, transplant these genres into a South African township setting where gang violence dominates and life is cheap. They are driven by uniquely South African characters, brutal crime scenes and fear-inspiring suspense, but none the less still full of humour. I want my work to entertain the reader while also looking realistically and critically at the problem of crime in our townships. I draw on influences of African and Latin American writers to create South African crime fiction in a realistic urban setting, with dynamic characters and sharp dialogue. , Le ngqokelela yamabali iqulathe amabali angobomi babantu abasezilokishini nabo bahlala ezilalini. Nangona umfundi angahle awafumanisa ehlekisa amanye elusizi, injongo yombhali asikukuhlekisa nakunyanzelisa imfundiso koko ikuzoba ubomi bababantu, bephila kwezi ndawo neengxaki abajongene nazo. Imeko yaba bantu kumakhaya ngamakhaya yiyo ebangele ukuba umbhali abelane nomfundi ngokuqhubekayo ebomini. , This thesis is presented in two parts: English and isiXhosa
- Full Text:
- Date Issued: 2017
- Authors: Fundakubi, Zukile Anthony
- Date: 2017
- Subjects: Creative writing (Higher education) South Africa , South African fiction (English) 21st century , Short stories, South African (English) 21st century , Xhosa fiction 21st century , Short stories, Xhosa 21st century , Detective and mystery stories 21st century
- Language: English , Xhosa
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/5071 , vital:20763
- Description: My writing contains elements of hard-boiled detective fiction and crime writing. My stories, written in isiXhosa and English and a mixture of both, transplant these genres into a South African township setting where gang violence dominates and life is cheap. They are driven by uniquely South African characters, brutal crime scenes and fear-inspiring suspense, but none the less still full of humour. I want my work to entertain the reader while also looking realistically and critically at the problem of crime in our townships. I draw on influences of African and Latin American writers to create South African crime fiction in a realistic urban setting, with dynamic characters and sharp dialogue. , Le ngqokelela yamabali iqulathe amabali angobomi babantu abasezilokishini nabo bahlala ezilalini. Nangona umfundi angahle awafumanisa ehlekisa amanye elusizi, injongo yombhali asikukuhlekisa nakunyanzelisa imfundiso koko ikuzoba ubomi bababantu, bephila kwezi ndawo neengxaki abajongene nazo. Imeko yaba bantu kumakhaya ngamakhaya yiyo ebangele ukuba umbhali abelane nomfundi ngokuqhubekayo ebomini. , This thesis is presented in two parts: English and isiXhosa
- Full Text:
- Date Issued: 2017
Being young, black, woman academics on an Accelerated Development Programme in an Historically White University in South Africa: a narrative analysis
- Mohoto, Nkoe Lieketso Paballo
- Authors: Mohoto, Nkoe Lieketso Paballo
- Date: 2017
- Subjects: New generation academic professionals Programme (South Africa) , College teachers, Black -- South Africa , Women college teachers, Black -- South Africa -- Case studies , Rhodes University
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/13202 , vital:21813
- Description: The national program for the development of next and new generation academic professionals (NGAP) aims to help Universities to diversify their academic teaching staff to be more reflective of the national demographics of the country. Through NGAP and policies of redress, a Historically White University would predictably introduce young black women into their academic teaching staff. This is a category of the population who would have been most affected by the exclusionary hiring policies that would have generally been in use in historically white universities before 1995, the year following the first democratic elections. The selection of staff according to criteria that has historically been used to exclude them is a policy which is widely considered to be a useful and necessary way to institute redress. While this half thesis does not disagree with this social and moral imperative, I find interest in the lack of focus on the emotional, psychological, spiritual and otherwise personal toll of the implementation of such a policy on those who are introduced through it and related policies. I believe there is a need to problematise the highly normative environments in which staff (to benefit from redress) are required to function. This half thesis examines the narrated experiences of three such staff members at Rhodes University with specific interest in their everyday experiences in an institution which has historically been tailored for (and in many cases is still run by) white, older male academics. The thesis indicates that the emotional and psychological effects and 'taxes' of being on an accelerated development programme may be worth noting and appreciating in order to think about the retention of black woman academics. The findings show that the complexity of younger black women's experiences within historically white universities such as Rhodes University requires equally complex and multifaceted strategies and programmes. These programmes should not only support these academics but also undermine existing exclusionary institutional cultures in order to facilitate true, deep transformational practice in historically white universities such as Rhodes University.
- Full Text:
- Date Issued: 2017
- Authors: Mohoto, Nkoe Lieketso Paballo
- Date: 2017
- Subjects: New generation academic professionals Programme (South Africa) , College teachers, Black -- South Africa , Women college teachers, Black -- South Africa -- Case studies , Rhodes University
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/13202 , vital:21813
- Description: The national program for the development of next and new generation academic professionals (NGAP) aims to help Universities to diversify their academic teaching staff to be more reflective of the national demographics of the country. Through NGAP and policies of redress, a Historically White University would predictably introduce young black women into their academic teaching staff. This is a category of the population who would have been most affected by the exclusionary hiring policies that would have generally been in use in historically white universities before 1995, the year following the first democratic elections. The selection of staff according to criteria that has historically been used to exclude them is a policy which is widely considered to be a useful and necessary way to institute redress. While this half thesis does not disagree with this social and moral imperative, I find interest in the lack of focus on the emotional, psychological, spiritual and otherwise personal toll of the implementation of such a policy on those who are introduced through it and related policies. I believe there is a need to problematise the highly normative environments in which staff (to benefit from redress) are required to function. This half thesis examines the narrated experiences of three such staff members at Rhodes University with specific interest in their everyday experiences in an institution which has historically been tailored for (and in many cases is still run by) white, older male academics. The thesis indicates that the emotional and psychological effects and 'taxes' of being on an accelerated development programme may be worth noting and appreciating in order to think about the retention of black woman academics. The findings show that the complexity of younger black women's experiences within historically white universities such as Rhodes University requires equally complex and multifaceted strategies and programmes. These programmes should not only support these academics but also undermine existing exclusionary institutional cultures in order to facilitate true, deep transformational practice in historically white universities such as Rhodes University.
- Full Text:
- Date Issued: 2017
Between reproductive rights and access to reproductive healthcare services: narratives of reproductive rights activists in South Africa
- Authors: Jacobs, Marc
- Date: 2017
- Subjects: Reproductive rights -- South Africa , Reproductive rights -- Political aspects -- South Africa , Human reproduction -- Law and legislation -- South Africa , Reproductive health -- South Africa , Abortion -- Government policy -- South Africa , South Africa. Choice of Termination of Pregnancy Act, 1996
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/17707 , vital:22270
- Description: The motivation behind the research paper, “Between reproductive rights and access to reproductive healthcare services: Narratives of reproductive rights activists in South Africa” stems from the notion that civil society groups have often played a critical role in addressing social justice issues concerning women’s rights. Since 1996 after implementation of the Choice on Termination of Pregnancy (CTOP) Act, South Africa has frequently been praised for its progressive abortion laws that formally recognises women’s need for reproductive autonomy and equality. However, ineffective implementation has resulted in many women facing a combination of barriers to accessing reproductive healthcare services. Thus, resulting in some women opting for unsafe, illegal abortion services and placing their health and lives in danger. The purpose of the study, therefore, is to capture the perceptions of contemporary South African based reproductive rights activists and NGOs who engage in campaigns that seek to highlight the complex relationship between reproductive rights and access to such rights. Data for the study was collected through open-ended questionnaires in which participants provided their unique opinions as activists who regularly engage with the abortion and access issues in South Africa. Results from the study suggest that ineffective implementation of the CTOP Act can be attributed to a lack of political will to prioritise women’s sexual and reproductive rights, thereby further marginalising women in society. Results also point to the need for widespread support from civil society on women’s rights matters so that the State can more effectively be held accountable for catering to the most marginalised women in South African society.
- Full Text:
- Date Issued: 2017
- Authors: Jacobs, Marc
- Date: 2017
- Subjects: Reproductive rights -- South Africa , Reproductive rights -- Political aspects -- South Africa , Human reproduction -- Law and legislation -- South Africa , Reproductive health -- South Africa , Abortion -- Government policy -- South Africa , South Africa. Choice of Termination of Pregnancy Act, 1996
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/17707 , vital:22270
- Description: The motivation behind the research paper, “Between reproductive rights and access to reproductive healthcare services: Narratives of reproductive rights activists in South Africa” stems from the notion that civil society groups have often played a critical role in addressing social justice issues concerning women’s rights. Since 1996 after implementation of the Choice on Termination of Pregnancy (CTOP) Act, South Africa has frequently been praised for its progressive abortion laws that formally recognises women’s need for reproductive autonomy and equality. However, ineffective implementation has resulted in many women facing a combination of barriers to accessing reproductive healthcare services. Thus, resulting in some women opting for unsafe, illegal abortion services and placing their health and lives in danger. The purpose of the study, therefore, is to capture the perceptions of contemporary South African based reproductive rights activists and NGOs who engage in campaigns that seek to highlight the complex relationship between reproductive rights and access to such rights. Data for the study was collected through open-ended questionnaires in which participants provided their unique opinions as activists who regularly engage with the abortion and access issues in South Africa. Results from the study suggest that ineffective implementation of the CTOP Act can be attributed to a lack of political will to prioritise women’s sexual and reproductive rights, thereby further marginalising women in society. Results also point to the need for widespread support from civil society on women’s rights matters so that the State can more effectively be held accountable for catering to the most marginalised women in South African society.
- Full Text:
- Date Issued: 2017
Beyond War, Violence, and Suffering: Everyday Life in the Honde Valley Borderland Communities during Zimbabwe’s Liberation War and the RENAMO Insurgency, c.1960-2016
- Authors: Nyachega, Nicholas
- Date: 2017
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/7023 , vital:21210
- Description: This thesis examines the history of the Honde Valley area, in Mutasa District, along the Zimbabwe-Mozambique border. It uses two historic developments: the Zimbabwe liberation war and RENAMO insurgency to explore daily life and mundane experiences of the borderland communities, mainly from the late 1970s to 2016. Because earlier historians of these two historic developments have been much interested in studying the aspects of violence and suffering, this study extends the focus of analysis to the mundane experiences. I argue that in borderland areas, there are other wartime aspects of life worth investigating other than violence and suffering. In doing so, the thesis deploys the notions of conviviality and the everyday to understand the daily experiences of the Honde Valley communities during the disruptions caused to everyday life by these wars. Admittedly, twentieth century wars in Zimbabwe and Mozambique transformed the area that had previously remained at the fringes of colonial power from 1890-1950, into a new and bitterly contested ‘sharp end’ of the war. Nonetheless, peoples’ experiences during these wars cannot be understood merely in relation to violence and suffering. Furthermore, I argue that although some families were forcibly moved into liberation war “Protected Villages ”, they innovatively designed new mechanisms and alternative lifestyles in response to the state’s routinised control. The thesis concludes that beyond the confines of war-induced violence and suffering, Honde Valley communities used their borderland location to evade the pressures of war and continued with life.
- Full Text:
- Date Issued: 2017
- Authors: Nyachega, Nicholas
- Date: 2017
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/7023 , vital:21210
- Description: This thesis examines the history of the Honde Valley area, in Mutasa District, along the Zimbabwe-Mozambique border. It uses two historic developments: the Zimbabwe liberation war and RENAMO insurgency to explore daily life and mundane experiences of the borderland communities, mainly from the late 1970s to 2016. Because earlier historians of these two historic developments have been much interested in studying the aspects of violence and suffering, this study extends the focus of analysis to the mundane experiences. I argue that in borderland areas, there are other wartime aspects of life worth investigating other than violence and suffering. In doing so, the thesis deploys the notions of conviviality and the everyday to understand the daily experiences of the Honde Valley communities during the disruptions caused to everyday life by these wars. Admittedly, twentieth century wars in Zimbabwe and Mozambique transformed the area that had previously remained at the fringes of colonial power from 1890-1950, into a new and bitterly contested ‘sharp end’ of the war. Nonetheless, peoples’ experiences during these wars cannot be understood merely in relation to violence and suffering. Furthermore, I argue that although some families were forcibly moved into liberation war “Protected Villages ”, they innovatively designed new mechanisms and alternative lifestyles in response to the state’s routinised control. The thesis concludes that beyond the confines of war-induced violence and suffering, Honde Valley communities used their borderland location to evade the pressures of war and continued with life.
- Full Text:
- Date Issued: 2017
Biogeomorphic aspects of lichen colonisation in part of western Dronning Maud Land, Antarctica
- Authors: Ayres, Gabrielle Alexzena
- Date: 2017
- Subjects: Lichen communities -- Queen Maud Land , Lichens -- Ecology -- Queen Maud Land , Polar regions , Ecology -- Polar regions , Antarctica , Ecology -- Antarctica
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/19713 , vital:22477
- Description: Ice-free regions in Antarctica provide natural laboratories for investigating and modelling change in landscapes and ecosystems. The terrestrial ecosystems in western Dronning Maud Land are potentially critical to improve current understanding of polar ecosystems, however, they remain poorly represented and under-protected. Lichen habitats were used to elucidate geomorphic-biological feedbacks that shape the landscape’s topography and biodiversity, which in turn promote environmental heterogeneity. Environmental parameters acting on two landforms were measured to determine the colonisation and growth of lichens in niche habitats. The habitats were identified and characterised, and topographic heterogeneity was found to control lichen colonisation. Micro-topographical niches provide the most stable environments for colonisation, and are preferred habitats. Lichen-habitat interactions were species specific, and closely related to insolation and moisture availability. The bidirectional relationships between rock weathering and lichen colonisation was ascertained. Results showed that lichens are good indicators of landform heterogeneity, but also promote landform heterogeneity. Given the habitat preferences of specific lichen species, they provide proxy to evaluating environmental responses, especially environmental change. The findings of this research are to be used as baseline data for future studies exploring ecosystem development in emerging landforms.
- Full Text:
- Date Issued: 2017
- Authors: Ayres, Gabrielle Alexzena
- Date: 2017
- Subjects: Lichen communities -- Queen Maud Land , Lichens -- Ecology -- Queen Maud Land , Polar regions , Ecology -- Polar regions , Antarctica , Ecology -- Antarctica
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/19713 , vital:22477
- Description: Ice-free regions in Antarctica provide natural laboratories for investigating and modelling change in landscapes and ecosystems. The terrestrial ecosystems in western Dronning Maud Land are potentially critical to improve current understanding of polar ecosystems, however, they remain poorly represented and under-protected. Lichen habitats were used to elucidate geomorphic-biological feedbacks that shape the landscape’s topography and biodiversity, which in turn promote environmental heterogeneity. Environmental parameters acting on two landforms were measured to determine the colonisation and growth of lichens in niche habitats. The habitats were identified and characterised, and topographic heterogeneity was found to control lichen colonisation. Micro-topographical niches provide the most stable environments for colonisation, and are preferred habitats. Lichen-habitat interactions were species specific, and closely related to insolation and moisture availability. The bidirectional relationships between rock weathering and lichen colonisation was ascertained. Results showed that lichens are good indicators of landform heterogeneity, but also promote landform heterogeneity. Given the habitat preferences of specific lichen species, they provide proxy to evaluating environmental responses, especially environmental change. The findings of this research are to be used as baseline data for future studies exploring ecosystem development in emerging landforms.
- Full Text:
- Date Issued: 2017
Biotic and abiotic factors promoting the development and proliferation of water hyacinth (eichhornia crassipes (Mart.) Solms-Laub.) in the Wouri Basin (Douala-Cameroon) and environs, with implications for its control
- Voukeng, Sonia Nadege Kenfack
- Authors: Voukeng, Sonia Nadege Kenfack
- Date: 2017
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/7644 , vital:21281
- Description: The Wouri River, situated in the Wouri Basin, is one of the main rivers of the Littoral Region in the city of Douala in Cameroon. It is a source of income and food for the population living around these areas. Since the 1990s, the fishing, transportation, irrigation and sand extraction activities have been impeded by the invasion of aquatic plants, specifically water hyacinth (Eichhornia crassipes [Mart.] Solms-Laubach: Pontederiaceae). Introduced in 1997 to the shore of Lake Chad, water hyacinth has invaded almost 114 ha of the Wouri Basin. Furthermore, Douala, the economic capital of the Cameroon and location for more than 70% of the country’s industries, uses the Wouri River and its tributaries to deposit its effluent and waste, which has worsened the problem of water hyacinth. This thesis examined the ecological and socio-economic impacts of water hyacinth in the Wouri Basin and its possible control. An increase in the nutrients in the water has provided water hyacinth with appropriate conditions for its fast growth during both the rainy and dry seasons. The availability of nutrients in these areas is enhanced by the constant, daily tidal fluctuation of water, providing enough water to the plant for easy nutrient uptake. A survey of the impacts of water hyacinth on aquatic plant communities in the Wouri Basin showed that this plant is able to out-compete native species. Assessment of the impact of water hyacinth on the abundance and diversity of plant communities indicated that at some invaded sites, 65% of the vegetation consisted of water hyacinth. Species found in association with water hyacinth with a high level of abundance-dominance were Pistia stratiotes L. (Araceae) (another invader), Commelina benghalensis L. (Commelinaceae) and Echinochloa pyramidalis (Lam.) Hitchc. & Chase (Poaceae). This component of the study also showed that habitats rich in water hyacinth were poor in diversity, while habitats without water hyacinth were rich in diversity, thus raising awareness of the importance of monitoring invasive aquatic weeds along the Wouri Basin, and of implementing correct control management of all invasive aquatic weeds. Communities living along the invaded rivers are well aware of the range of problems caused by the weed; because as the rivers and water bodies used for fishing, transportation, and sand extraction are progressively invaded by the weed, the riparian population is the first to feel the impact. The impact on people has been noticeable, with an increase in diseases, such as malaria, cholera, diarrhoea, typhoid, filariasis, schistosomiasis, scabies and yellow fever increasing the need for a medicine and hospitalization. Economic losses due to the management of invasive aquatic weeds were recorded, and the Ministry of Environment spent an estimated US$1 200 000 between 2010 and 2015 to manage this scourge. In 2016, an amount of US$160 000 was transferred to these regions to manage invasive aquatic weeds, especially water hyacinth, although manual clearing is still the only method used to control this weed. Isolation of fungi from diseased water hyacinth plants in the Wouri Basin revealed several fungal species, most of which have been isolated from water hyacinth species in water bodies elsewhere, which showed a higher diversity during the dry season than during the rainy season. These fungi included Acremonium zonatum (Sawada). W. Gams (Hypocreaceae), Alternaria eichhorniae Nag Raj & Ponnappa (Pleosporaceae), Chaetomium sp., Colletotrichum sp., Curvularia pallescens Boedjin (Pleosporaceae), Curvalaria sp., Epicoccum nigrum Link (Pleosporaceae), Fusarium sp., Pithomyces chartarum fBerk. & M. A. Curtis) M. B. Ellis (Montagnulaceae), to a lesser extent Myrothecium roridum Tode ex Fr. (Incertae sedis) and Nigrospora sp. Although never released in Cameroon, arthropod biological control agents (Neochetina eichhorniae Warner (Coleoptera, Curculionidae) and N. bruchi Hustache (Coleoptera, Curculionidae)) were present, but their populations were relatively low. The slow spread of the insect population was explained by several factors, among them the tidal fluctuation of water, which has an impact on the population growth of the weevils. Whilst adults may be able to survive tidal fluctuations, larvae are severely impacted by them, contributing to the slow success of biological control. In this study, a significant increase in pathogen-induced disease severity and incidence was noted when Neochetina eichhorniae weevils were present, possibly because larvae tunnelling on the petiole created openings for the penetration of fungal spores. This study highlights the negative impacts of water hyacinth, on the environment, people, and thus economy of Cameroon. The presence of biological control agents and pathogens offers Cameroon the possibility of initiating and properly implementing the biological control option, or an integrated management solution, to manage water hyacinth in the Wouri Basin, and in the rest of Cameroon.
- Full Text:
- Date Issued: 2017
- Authors: Voukeng, Sonia Nadege Kenfack
- Date: 2017
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/7644 , vital:21281
- Description: The Wouri River, situated in the Wouri Basin, is one of the main rivers of the Littoral Region in the city of Douala in Cameroon. It is a source of income and food for the population living around these areas. Since the 1990s, the fishing, transportation, irrigation and sand extraction activities have been impeded by the invasion of aquatic plants, specifically water hyacinth (Eichhornia crassipes [Mart.] Solms-Laubach: Pontederiaceae). Introduced in 1997 to the shore of Lake Chad, water hyacinth has invaded almost 114 ha of the Wouri Basin. Furthermore, Douala, the economic capital of the Cameroon and location for more than 70% of the country’s industries, uses the Wouri River and its tributaries to deposit its effluent and waste, which has worsened the problem of water hyacinth. This thesis examined the ecological and socio-economic impacts of water hyacinth in the Wouri Basin and its possible control. An increase in the nutrients in the water has provided water hyacinth with appropriate conditions for its fast growth during both the rainy and dry seasons. The availability of nutrients in these areas is enhanced by the constant, daily tidal fluctuation of water, providing enough water to the plant for easy nutrient uptake. A survey of the impacts of water hyacinth on aquatic plant communities in the Wouri Basin showed that this plant is able to out-compete native species. Assessment of the impact of water hyacinth on the abundance and diversity of plant communities indicated that at some invaded sites, 65% of the vegetation consisted of water hyacinth. Species found in association with water hyacinth with a high level of abundance-dominance were Pistia stratiotes L. (Araceae) (another invader), Commelina benghalensis L. (Commelinaceae) and Echinochloa pyramidalis (Lam.) Hitchc. & Chase (Poaceae). This component of the study also showed that habitats rich in water hyacinth were poor in diversity, while habitats without water hyacinth were rich in diversity, thus raising awareness of the importance of monitoring invasive aquatic weeds along the Wouri Basin, and of implementing correct control management of all invasive aquatic weeds. Communities living along the invaded rivers are well aware of the range of problems caused by the weed; because as the rivers and water bodies used for fishing, transportation, and sand extraction are progressively invaded by the weed, the riparian population is the first to feel the impact. The impact on people has been noticeable, with an increase in diseases, such as malaria, cholera, diarrhoea, typhoid, filariasis, schistosomiasis, scabies and yellow fever increasing the need for a medicine and hospitalization. Economic losses due to the management of invasive aquatic weeds were recorded, and the Ministry of Environment spent an estimated US$1 200 000 between 2010 and 2015 to manage this scourge. In 2016, an amount of US$160 000 was transferred to these regions to manage invasive aquatic weeds, especially water hyacinth, although manual clearing is still the only method used to control this weed. Isolation of fungi from diseased water hyacinth plants in the Wouri Basin revealed several fungal species, most of which have been isolated from water hyacinth species in water bodies elsewhere, which showed a higher diversity during the dry season than during the rainy season. These fungi included Acremonium zonatum (Sawada). W. Gams (Hypocreaceae), Alternaria eichhorniae Nag Raj & Ponnappa (Pleosporaceae), Chaetomium sp., Colletotrichum sp., Curvularia pallescens Boedjin (Pleosporaceae), Curvalaria sp., Epicoccum nigrum Link (Pleosporaceae), Fusarium sp., Pithomyces chartarum fBerk. & M. A. Curtis) M. B. Ellis (Montagnulaceae), to a lesser extent Myrothecium roridum Tode ex Fr. (Incertae sedis) and Nigrospora sp. Although never released in Cameroon, arthropod biological control agents (Neochetina eichhorniae Warner (Coleoptera, Curculionidae) and N. bruchi Hustache (Coleoptera, Curculionidae)) were present, but their populations were relatively low. The slow spread of the insect population was explained by several factors, among them the tidal fluctuation of water, which has an impact on the population growth of the weevils. Whilst adults may be able to survive tidal fluctuations, larvae are severely impacted by them, contributing to the slow success of biological control. In this study, a significant increase in pathogen-induced disease severity and incidence was noted when Neochetina eichhorniae weevils were present, possibly because larvae tunnelling on the petiole created openings for the penetration of fungal spores. This study highlights the negative impacts of water hyacinth, on the environment, people, and thus economy of Cameroon. The presence of biological control agents and pathogens offers Cameroon the possibility of initiating and properly implementing the biological control option, or an integrated management solution, to manage water hyacinth in the Wouri Basin, and in the rest of Cameroon.
- Full Text:
- Date Issued: 2017
Bird-Monk Seding
- Authors: Rampolokeng, Lesego
- Date: 2017
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/5049 , vital:20758
- Description: This novella is made up of interlinked stories from a rural township in the North West province. The stories of this surface-tranquil place are told through descriptive passages, vignettes, snatches of dialogue, profiles and picture-postcards, all organically interwoven and entwined, and rendered in non-linear fashion. They are set in shebeens, shops, farmlands and the dusty empty spaces of the South African landscape, peopled by police, tourists, and prostitutes of all sorts. The pervasiveness of violence in all forms has the fictional narrator reflecting on the violence of his own past. A smattering of musicians' musings gleaned from interviews and album liner-notes helps him navigate his way through this morass, the rage and frustration that simmers beneath it all.
- Full Text:
- Date Issued: 2017
- Authors: Rampolokeng, Lesego
- Date: 2017
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/5049 , vital:20758
- Description: This novella is made up of interlinked stories from a rural township in the North West province. The stories of this surface-tranquil place are told through descriptive passages, vignettes, snatches of dialogue, profiles and picture-postcards, all organically interwoven and entwined, and rendered in non-linear fashion. They are set in shebeens, shops, farmlands and the dusty empty spaces of the South African landscape, peopled by police, tourists, and prostitutes of all sorts. The pervasiveness of violence in all forms has the fictional narrator reflecting on the violence of his own past. A smattering of musicians' musings gleaned from interviews and album liner-notes helps him navigate his way through this morass, the rage and frustration that simmers beneath it all.
- Full Text:
- Date Issued: 2017
Browsing as a demographic bottleneck in a semi-arid savanna: the effect of size and age on compensatory responses of Vachellia karroo seedlings after simulated herbivory
- Authors: Perumal, Lavinia
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4899 , vital:20741
- Description: Savannas are characterised by a continuous grass layer with scattered trees at varying densities. This vegetation structure is determined by several interacting factors, including fire, herbivory, resource competition and atmospheric CO2 concentration. The preservation of savanna biomes is important and a shift towards a woody biome threatens savanna globally. Bush encroachment which describes the shift towards domination of savannas by C3 woody plants, is especially acute in southern Africa. In semi-arid rangelands, encroachment progresses to dense thickets dominated by thorny and unpalatable bushes and trees. There is evidence that bush encroachment is driven by a reduction in fire and browsing events as well as their interaction. Despite browsing having strong effects on African savannas its isolated role in maintaining tree-grass coexistence has not received as much attention as the role of fire. Therefore the overall aim of this study was to examine the effects of browsing on seedlings of a commonly encroaching species, Vachellia karroo. Browsing was hypothesized to be a demographic release bottleneck for bush encroachment in a semiarid (MAP ~550mm) savanna in the Eastern Cape of South Africa, where fire has been historically rare. In a single study I explored the fate of V. karroo seedlings (less than a year old) following browsing in sub canopy and inter canopy microhabitats. Additionally, I explored how the fate of a seedling changed under high and low tree cover. Firstly, I investigated the type, intensity and frequency of herbivory, from both small and large herbivores, which seedlings were subjected to. Results revealed that browsing was severe and frequent with the majority of seedlings browsed more than twice over a 12 month period. Large browsers such as kudu and impala caused high seedling mortality (46%) while smaller browsers such as invertebrates were more effective at suppressing growth. Microhabitat had little impact on seedling survival, but significantly influenced plant compensatory growth. Reduced seedling growth following browsing was observed in the sub-canopy in comparison to seedling growth in full sunlight in the intercanopy, suggesting V.karroo may be shade intolerant. Secondly, the effect of tree cover on browsed seedlings was determined by quantifying browsing frequency and intensity at high and low tree cover. No differences in browsing intensity and frequency were observed between high and low tree cover. However, high tree cover due to bush encroachment limited seedling above ground growth. The aim of the second study was to investigate how V.karroo survival and growth were influenced by its age and size following simulated browsing. I explored this aim through field and greenhouse experiments. I was particularly interested in testing how plant sensitivity to varying defoliation intensities of repeated browsing varied with plant age (known ages of 6, 12, 16 and 30 weeks). There were large differences in mortality between the different age groups. Furthermore, age interacted with repeated browsing and negatively influenced seedling survival and regrowth. Older seedlings (16 and 30 week old) had greater survival and higher browsing frequencies resulted in greater mortality and reduced growth. The threshold age after which seedlings become more tolerant to herbivory occurs at an age of 28 weeks. Seedlings less than six weeks old experiencing intense (100 % defoliation)browsing had a very low probability (33%) of survival following just a single defoliation. Interestingly, all 16 week old seedlings regrew most of their foliage following a moderate (50%) defoliation with some plants overcompensating for leaf loss. The field study revealed two distinct demographic stages based on age and size (seedlings< 9 mm and saplings >9 mm in stem basal diameter (SBD)). Browsing had a strong negative effect on seedlings, resulting in reduced investment in leaf biomass. These findings suggest plant size and age can be used as robust predictors of a plants vulnerability to browsing. An increase in tree cover requires successful transitions of seedlings to saplings (also known as release). The results of this study suggest that in semi-arid savannas, browsing can impact tree cover through imposing a release bottleneck for tree seedlings and to lesser extent saplings. By limiting tree growth plants are kept in a disturbance trap and will therefore be exposed to not only browsers but fire. These findings also have important implications for tree-grass coexistence dynamics, suggesting that specific size and browsing thresholds should be considered in savanna management.
- Full Text:
- Date Issued: 2017
- Authors: Perumal, Lavinia
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4899 , vital:20741
- Description: Savannas are characterised by a continuous grass layer with scattered trees at varying densities. This vegetation structure is determined by several interacting factors, including fire, herbivory, resource competition and atmospheric CO2 concentration. The preservation of savanna biomes is important and a shift towards a woody biome threatens savanna globally. Bush encroachment which describes the shift towards domination of savannas by C3 woody plants, is especially acute in southern Africa. In semi-arid rangelands, encroachment progresses to dense thickets dominated by thorny and unpalatable bushes and trees. There is evidence that bush encroachment is driven by a reduction in fire and browsing events as well as their interaction. Despite browsing having strong effects on African savannas its isolated role in maintaining tree-grass coexistence has not received as much attention as the role of fire. Therefore the overall aim of this study was to examine the effects of browsing on seedlings of a commonly encroaching species, Vachellia karroo. Browsing was hypothesized to be a demographic release bottleneck for bush encroachment in a semiarid (MAP ~550mm) savanna in the Eastern Cape of South Africa, where fire has been historically rare. In a single study I explored the fate of V. karroo seedlings (less than a year old) following browsing in sub canopy and inter canopy microhabitats. Additionally, I explored how the fate of a seedling changed under high and low tree cover. Firstly, I investigated the type, intensity and frequency of herbivory, from both small and large herbivores, which seedlings were subjected to. Results revealed that browsing was severe and frequent with the majority of seedlings browsed more than twice over a 12 month period. Large browsers such as kudu and impala caused high seedling mortality (46%) while smaller browsers such as invertebrates were more effective at suppressing growth. Microhabitat had little impact on seedling survival, but significantly influenced plant compensatory growth. Reduced seedling growth following browsing was observed in the sub-canopy in comparison to seedling growth in full sunlight in the intercanopy, suggesting V.karroo may be shade intolerant. Secondly, the effect of tree cover on browsed seedlings was determined by quantifying browsing frequency and intensity at high and low tree cover. No differences in browsing intensity and frequency were observed between high and low tree cover. However, high tree cover due to bush encroachment limited seedling above ground growth. The aim of the second study was to investigate how V.karroo survival and growth were influenced by its age and size following simulated browsing. I explored this aim through field and greenhouse experiments. I was particularly interested in testing how plant sensitivity to varying defoliation intensities of repeated browsing varied with plant age (known ages of 6, 12, 16 and 30 weeks). There were large differences in mortality between the different age groups. Furthermore, age interacted with repeated browsing and negatively influenced seedling survival and regrowth. Older seedlings (16 and 30 week old) had greater survival and higher browsing frequencies resulted in greater mortality and reduced growth. The threshold age after which seedlings become more tolerant to herbivory occurs at an age of 28 weeks. Seedlings less than six weeks old experiencing intense (100 % defoliation)browsing had a very low probability (33%) of survival following just a single defoliation. Interestingly, all 16 week old seedlings regrew most of their foliage following a moderate (50%) defoliation with some plants overcompensating for leaf loss. The field study revealed two distinct demographic stages based on age and size (seedlings< 9 mm and saplings >9 mm in stem basal diameter (SBD)). Browsing had a strong negative effect on seedlings, resulting in reduced investment in leaf biomass. These findings suggest plant size and age can be used as robust predictors of a plants vulnerability to browsing. An increase in tree cover requires successful transitions of seedlings to saplings (also known as release). The results of this study suggest that in semi-arid savannas, browsing can impact tree cover through imposing a release bottleneck for tree seedlings and to lesser extent saplings. By limiting tree growth plants are kept in a disturbance trap and will therefore be exposed to not only browsers but fire. These findings also have important implications for tree-grass coexistence dynamics, suggesting that specific size and browsing thresholds should be considered in savanna management.
- Full Text:
- Date Issued: 2017
Calibration and imaging with variable radio sources
- Authors: Mbou Sob, Ulrich Armel
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/37977 , vital:24721
- Description: Calibration of radio interferometric data is one of the most important steps that are required to produce high dynamic range radio maps with high fidelity. However, naive calibration (inaccurate knowledge of the sky and instruments) leads to the formation of calibration artefacts: the generation of spurious sources and the deformations in the structure of extended sources. A particular class of calibration artefacts, called ghost sources, which results from calibration with incomplete sky models has been extensively studied by Grobler et al. (2014, 2016) and Wijnholds et al. (2016). They developed a framework which can be used to predict the fluxes and positions of ghost sources. This work uses the approach initiated by these authors to study the calibration artefacts and ghost sources that are produced when variable sources are not considered in sky models during calibration. This work investigates both long-term and short-term variability and uses the root mean square (rms) and power spectrum as metrics to evaluate the “quality” of the residual visibilities obtained through calibration. We show that the overestimation and underestimation of source flux density during calibration produces similar but symmetrically opposite results. We show that calibration artefacts from sky model errors are not normally distributed. This prevents them from being removed by employing advanced techniques, such as stacking. The power spectrums measured from the residuals with a variable source was significantly higher than those from residuals without a variable source. This implies advanced calibration techniques and sky model completeness will be required for studies such as probing the Epoch of Reoinization, where we seek to detect faint signals below thermal noise.
- Full Text:
- Date Issued: 2017
- Authors: Mbou Sob, Ulrich Armel
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/37977 , vital:24721
- Description: Calibration of radio interferometric data is one of the most important steps that are required to produce high dynamic range radio maps with high fidelity. However, naive calibration (inaccurate knowledge of the sky and instruments) leads to the formation of calibration artefacts: the generation of spurious sources and the deformations in the structure of extended sources. A particular class of calibration artefacts, called ghost sources, which results from calibration with incomplete sky models has been extensively studied by Grobler et al. (2014, 2016) and Wijnholds et al. (2016). They developed a framework which can be used to predict the fluxes and positions of ghost sources. This work uses the approach initiated by these authors to study the calibration artefacts and ghost sources that are produced when variable sources are not considered in sky models during calibration. This work investigates both long-term and short-term variability and uses the root mean square (rms) and power spectrum as metrics to evaluate the “quality” of the residual visibilities obtained through calibration. We show that the overestimation and underestimation of source flux density during calibration produces similar but symmetrically opposite results. We show that calibration artefacts from sky model errors are not normally distributed. This prevents them from being removed by employing advanced techniques, such as stacking. The power spectrums measured from the residuals with a variable source was significantly higher than those from residuals without a variable source. This implies advanced calibration techniques and sky model completeness will be required for studies such as probing the Epoch of Reoinization, where we seek to detect faint signals below thermal noise.
- Full Text:
- Date Issued: 2017
Carbon credit restoration projects in the Eastern Cape province of South Africa: considerations for sustainable local economic development
- Authors: Polak, James Samuel
- Date: 2017
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/8069 , vital:21349
- Description: Although global climate change has been identified as a serious global economic, social, and environmental threat to society, national governments have been slow to respond on a global scale. Environmental economic theory offers market-based solutions to address climate change efficiently through carbon control regimes, such as carbon taxation and cap-and-trade policy. A major political milestone was reached when an international agreement entered into force in 2008, known the Kyoto Protocol, which incorporated a market-based solution to address climate change on a global scale. This allowed a global market for emissions to form through the Clean Development Mechanism. Although the Kyoto Protocol aimed to address a global issue on a global scale through a single global market for emissions, fragmented sovereign cap-and-trade schemes have since emerged in the form of national and regional emission markets, commonly referred to as carbon markets. The Clean Development Mechanism offered the opportunity to generate carbon credits through carbon offset projects, such as carbon restoration projects. Although the Kyoto Protocol did not achieve the objective of forming an internationally accepted global carbon control regime, it seems to have set a trend of including offset programs in newly emerging carbon control regimes, such as South Africa’s proposed carbon tax. This study set out to assess the extent to which carbon control regimes are enabling sustainable local economic development, based on carbon restoration projects in the Eastern Cape province of South Africa using Portulacaria afra, commonly known as spekboom. As a starting point, this study assessed the current state of the international carbon markets using significant international cap-and-trade based markets as examples. Based on Newell et al.’s (2013) selection of significant carbon markets and data availability, the United States’ Regional Greenhouse Gas Initiative and the European Union’s Emission Trading Scheme were selected. Historical, current and forecasted supply and demand data were gathered from Thomson Reuters’ Point Carbon research division. Further, historical futures and spot market price and volume data were gathered from the markets to compare how prices have fared over time. The Clean Development Mechanism’s market for Certified Emission Reduction credits was used for comparative purposes. The markets were found to be systemically oversupplied, leading to systemically low prices. The systemic oversupply in credits provides a limited incentive to initiate carbon offset projects, however, South Africa’s proposed carbon tax may be able to stimulate demand for domestic offset projects. Key success factors established through a comparative literature review on local economic development theory were incorporated into key informant interviews. The results were then analysed through the lens of Connelly’s (2007) model for sustainable development to provide recommendations for sustainable local economic development, regarding carbon restoration projects in the Eastern Cape using P. afra. The following opportunities were identified: the planting of P. afra on degraded land has the potential to mitigate climate change, offer water benefits to the surrounding communities, and promote biodiversity regeneration. There is potential for economic growth through job creation and the economic multiplier effect. Government funding is available and voluntary offset agreements do exist, eliminating a large portion of the cumbersome accreditation requirements. These projects also offer potential for social justice through the government funding requirements which stipulate that youth and women should be given preference for employment, potentially helping to alleviate inequality. The funding further stipulates that employees should be trained in transferable skills, offering potential for capacity building and social capital accumulation through education. These transferable skills include skills geared towards encouraging entrepreneurialism. Corresponding challenges were also found: excessive overgrazing through pastoralism has rendered some land degraded beyond restoration. Opportunists may resort to planting P. afra outside of the subtropical Albany Thicket biome to which it is endemic, leading to potential biodiversity loss rather than gains. Sufficient buy-in is required from private landowners for these projects to be sustainable, however, the projects entail a large opportunity cost to farmers as returns take at least 5 to 6 years. This may render these projects undesirable to most landowners and provides scope for free-riding, should pastoralists not have to bear the full costs of the project and property rights not be enforced through land user agreements. The projects require an exorbitant amount of upfront funding. Cash flow received from the projects does not extend in perpetuity. Requirements for social justice pertain only to government funded projects. A working model, generating and selling carbon credits through land restoration using P. afra, has not yet been established. As it stands, these carbon restoration projects are still highly speculative and carry a significant amount of investment risk, given the high mortality and low growth rates associated with the current planting method. The current systemic oversupply of carbon. credits in the international markets signal that returns from carbon credits are set to be low, at least until the oversupply issues are resolved. Should these challenges be overcome, carbon restoration projects using P. afra may have the potential to bolster sustainable local economic development in the impoverished regions of province as well as provide a locally-driven adaptation and mitigation strategy to address global climate change.
- Full Text:
- Date Issued: 2017
- Authors: Polak, James Samuel
- Date: 2017
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/8069 , vital:21349
- Description: Although global climate change has been identified as a serious global economic, social, and environmental threat to society, national governments have been slow to respond on a global scale. Environmental economic theory offers market-based solutions to address climate change efficiently through carbon control regimes, such as carbon taxation and cap-and-trade policy. A major political milestone was reached when an international agreement entered into force in 2008, known the Kyoto Protocol, which incorporated a market-based solution to address climate change on a global scale. This allowed a global market for emissions to form through the Clean Development Mechanism. Although the Kyoto Protocol aimed to address a global issue on a global scale through a single global market for emissions, fragmented sovereign cap-and-trade schemes have since emerged in the form of national and regional emission markets, commonly referred to as carbon markets. The Clean Development Mechanism offered the opportunity to generate carbon credits through carbon offset projects, such as carbon restoration projects. Although the Kyoto Protocol did not achieve the objective of forming an internationally accepted global carbon control regime, it seems to have set a trend of including offset programs in newly emerging carbon control regimes, such as South Africa’s proposed carbon tax. This study set out to assess the extent to which carbon control regimes are enabling sustainable local economic development, based on carbon restoration projects in the Eastern Cape province of South Africa using Portulacaria afra, commonly known as spekboom. As a starting point, this study assessed the current state of the international carbon markets using significant international cap-and-trade based markets as examples. Based on Newell et al.’s (2013) selection of significant carbon markets and data availability, the United States’ Regional Greenhouse Gas Initiative and the European Union’s Emission Trading Scheme were selected. Historical, current and forecasted supply and demand data were gathered from Thomson Reuters’ Point Carbon research division. Further, historical futures and spot market price and volume data were gathered from the markets to compare how prices have fared over time. The Clean Development Mechanism’s market for Certified Emission Reduction credits was used for comparative purposes. The markets were found to be systemically oversupplied, leading to systemically low prices. The systemic oversupply in credits provides a limited incentive to initiate carbon offset projects, however, South Africa’s proposed carbon tax may be able to stimulate demand for domestic offset projects. Key success factors established through a comparative literature review on local economic development theory were incorporated into key informant interviews. The results were then analysed through the lens of Connelly’s (2007) model for sustainable development to provide recommendations for sustainable local economic development, regarding carbon restoration projects in the Eastern Cape using P. afra. The following opportunities were identified: the planting of P. afra on degraded land has the potential to mitigate climate change, offer water benefits to the surrounding communities, and promote biodiversity regeneration. There is potential for economic growth through job creation and the economic multiplier effect. Government funding is available and voluntary offset agreements do exist, eliminating a large portion of the cumbersome accreditation requirements. These projects also offer potential for social justice through the government funding requirements which stipulate that youth and women should be given preference for employment, potentially helping to alleviate inequality. The funding further stipulates that employees should be trained in transferable skills, offering potential for capacity building and social capital accumulation through education. These transferable skills include skills geared towards encouraging entrepreneurialism. Corresponding challenges were also found: excessive overgrazing through pastoralism has rendered some land degraded beyond restoration. Opportunists may resort to planting P. afra outside of the subtropical Albany Thicket biome to which it is endemic, leading to potential biodiversity loss rather than gains. Sufficient buy-in is required from private landowners for these projects to be sustainable, however, the projects entail a large opportunity cost to farmers as returns take at least 5 to 6 years. This may render these projects undesirable to most landowners and provides scope for free-riding, should pastoralists not have to bear the full costs of the project and property rights not be enforced through land user agreements. The projects require an exorbitant amount of upfront funding. Cash flow received from the projects does not extend in perpetuity. Requirements for social justice pertain only to government funded projects. A working model, generating and selling carbon credits through land restoration using P. afra, has not yet been established. As it stands, these carbon restoration projects are still highly speculative and carry a significant amount of investment risk, given the high mortality and low growth rates associated with the current planting method. The current systemic oversupply of carbon. credits in the international markets signal that returns from carbon credits are set to be low, at least until the oversupply issues are resolved. Should these challenges be overcome, carbon restoration projects using P. afra may have the potential to bolster sustainable local economic development in the impoverished regions of province as well as provide a locally-driven adaptation and mitigation strategy to address global climate change.
- Full Text:
- Date Issued: 2017
Cardiovascular disease risk in Black and Caucasian females: an Eastern Cape sample
- Authors: Remsing, Sandra Claudia
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/40737 , vital:25021
- Description: The purpose of this study was to investigate cardiovascular disease risk in Black and Caucasian urban working females of the Makana region of the Eastern Cape. One- hundred and sixty six age-matched urban-working individuals (Black n = 88; Caucasian n = 78) voluntarily participated in this cross-sectional study. Cardiovascular disease risk was assessed in four categories: 1) obesity, classified as ‘morphological risk’, 2) blood pressure, classified as ‘cardiovascular risk’, 3) diet, physical activity, smoking, and alcohol consumption, classified as ‘lifestyle risk’, and 4) education and income, classified as ‘socioeconomic factors’. Results showed that Black females were significantly (p<0.01) heavier than Caucasian females and categorized as ‘obese’ according to BMI classifications (31.19 ± 8.09 kg.m2 and 25.90 ±4.67 kg.m2, respectively). Black females also presented with significantly (p<0.01) higher waist circumferences and waist-to-stature ratios, further confirming the obesity prevalence in this group. When accounting for those who were on anti-hypertensive mediation, Black females additionally presented with significantly (p<0.05) higher blood pressure, categorizing them as ‘pre-hypertensive’ (MAP = 98 ± 16 mmHg and 85 ± 11 mmHg, respectively). These results therefore placed Black females at increased cardiovascular disease risk compared to Caucasian females. With respect to lifestyle factors, Black females consumed significantly (p<0.05) more kilojoules carbohydrates, and total sugar than Caucasian females, and expended more physical activity MET- minutes per week (2688.86 ± 1486.56 MET-minutes and 1828.27 ± 2238.10 MET- minutes, respectively). However, reduced validity of physical activity data as well as poor dietary recall limited the interpretations of these findings. In addition, despite significant differences between smoking and alcohol consumption between groups, the majority of both Black and Caucasian females reported being non-smokers and consumed alcohol minimally. Thus, these were concluded to be unlikely contributors to cardiovascular disease risk in these samples. Socioeconomic results nevertheless expectedly showed that Black females were significantly (p<0.05) less educated, and subsequently earned lower incomes. It was thus concluded that this may be a likely contributor to higher cardiovascular risk in Black females. Future recommendations include the incorporation of exercise intensity measures as well as objective measures of habitual diet. This is in order to get a clearer understanding of the impact of these lifestyle factors on cardiovascular disease risk.
- Full Text:
- Date Issued: 2017
- Authors: Remsing, Sandra Claudia
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/40737 , vital:25021
- Description: The purpose of this study was to investigate cardiovascular disease risk in Black and Caucasian urban working females of the Makana region of the Eastern Cape. One- hundred and sixty six age-matched urban-working individuals (Black n = 88; Caucasian n = 78) voluntarily participated in this cross-sectional study. Cardiovascular disease risk was assessed in four categories: 1) obesity, classified as ‘morphological risk’, 2) blood pressure, classified as ‘cardiovascular risk’, 3) diet, physical activity, smoking, and alcohol consumption, classified as ‘lifestyle risk’, and 4) education and income, classified as ‘socioeconomic factors’. Results showed that Black females were significantly (p<0.01) heavier than Caucasian females and categorized as ‘obese’ according to BMI classifications (31.19 ± 8.09 kg.m2 and 25.90 ±4.67 kg.m2, respectively). Black females also presented with significantly (p<0.01) higher waist circumferences and waist-to-stature ratios, further confirming the obesity prevalence in this group. When accounting for those who were on anti-hypertensive mediation, Black females additionally presented with significantly (p<0.05) higher blood pressure, categorizing them as ‘pre-hypertensive’ (MAP = 98 ± 16 mmHg and 85 ± 11 mmHg, respectively). These results therefore placed Black females at increased cardiovascular disease risk compared to Caucasian females. With respect to lifestyle factors, Black females consumed significantly (p<0.05) more kilojoules carbohydrates, and total sugar than Caucasian females, and expended more physical activity MET- minutes per week (2688.86 ± 1486.56 MET-minutes and 1828.27 ± 2238.10 MET- minutes, respectively). However, reduced validity of physical activity data as well as poor dietary recall limited the interpretations of these findings. In addition, despite significant differences between smoking and alcohol consumption between groups, the majority of both Black and Caucasian females reported being non-smokers and consumed alcohol minimally. Thus, these were concluded to be unlikely contributors to cardiovascular disease risk in these samples. Socioeconomic results nevertheless expectedly showed that Black females were significantly (p<0.05) less educated, and subsequently earned lower incomes. It was thus concluded that this may be a likely contributor to higher cardiovascular risk in Black females. Future recommendations include the incorporation of exercise intensity measures as well as objective measures of habitual diet. This is in order to get a clearer understanding of the impact of these lifestyle factors on cardiovascular disease risk.
- Full Text:
- Date Issued: 2017
Changes in household food security, nutrition and food waste along an agro-ecological gradient and the rural-urban continuum in mid-sized South African towns
- Authors: Chakona, Gamuchirai
- Date: 2017
- Subjects: Food security -- South Africa , Women -- South Africa -- Nutritiona , Urban women -- South Africa -- Nutrition , Rural women -- South Africa -- Nutrition , Wild foods -- South Africa , Food supply -- South Africa , Malnutrition in children -- South Africa , Grants-in-aid -- South Africa , Household ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/6484 , vital:21127
- Description: The lack of dietary diversity is a severe problem experienced by most poor households globally. Most poor communities are at high risk of inadequate intake of micronutrients resulting from diets dominated by starchy staples. The present study considered the diets, dietary diversities and food security of women of reproductive age between 15-49 years, households and communities along the rural-urban continuum in three mid-sized towns situated along an agro-ecological gradient in South Africa. A 48-hour dietary recall was performed across two seasons (twice in summer and once in winter) and focus group discussions were held to gather all information including food abundance, seasons of food scarcity as well as coping strategies which households employ during periods of food shocks. Households were further asked to quantify any type of food waste that they could have generated in the previous 48 hours. Nutritional status of children under the age of five in all three towns was also measured using height-for-age (HAZ) and mid-upper arm circumference (MUAC) as indicators of stunting and wasting respectively. The household surveys were conducted with 554 women randomly selected in rural, peri-urban and urban locations of Richards Bay, Dundee and Harrismith. For nutritional status, the sample consisted of 216 children who were randomly selected from the sampled households in rural, peri-urban and urban locations of the study sites. Household Dietary Diversity Scores (HDDS) and Women’s Dietary Diversity Scores (WDDS) were calculated from the food items consumed by each household and each woman over a two-day period, respectively. Household food access was also measured for each household using Household Food Insecurity Access Scale (HFIAS). The mean WDDS and HDDS for the wettest site of Richards Bay (3.8±0.29 and 8.44±1.72, respectively) was significantly higher than at Dundee (3.4±0.30 and 7.76±1.63, respectively) and Harrismith (3.5±0.27 and 7.83±1.59, respectively) which were not different from one another. The mean HFIAS for Dundee (9.39±7.13) was significantly higher than that in Richards Bay (5.57±6.98) and Harrismith (6.43±6.59) which were not significantly different from one another. Dietary diversity scores were also significantly higher in urban locations than in peri-urban and rural ones whilst HFIAS was significantly lower in the urban locations than peri-urban and rural locations. There was lower dependence on food purchasing in Richards Bay compared to Dundee and Harrismith where the majority of the population was purchasing most of their food. The majority of Richards Bay households were involved in subsistence agriculture and also produced a surplus for sale, as well as collecting wild foods, which improved food security, unlike Dundee and Harrismith. Food insecurity was higher in rural and peri-urban areas compared to urban areas. In all towns, food was always available throughout the year but was beyond the reach of many households. In urban areas food was readily available and only limited by access, whereas rural populations have limited access to affordable food and face higher prices. The peri-urban populations were more food insecure because of high levels of poverty, unemployment and lack of access/entitlements to land. Periurban dwellers are therefore more sensitive to changes in incomes and food prices because they lack safety nets to absorb income or price shocks as they purchase more, rather than growing their own food. Household dietary diversity was significantly negatively correlated with household food access, that is households with low HDDS had higher HFIAS scores. Due to high levels of food insecurity, a greater percentage of children under the age of five years were stunted (35 %) and wasted (18 %). There were no significant differences in stunting along the agro-ecological gradient, along the rural-urban continuum and sex of child. However, significant differences were observed in child wasting along the agro-ecological gradient with Harrismith having more wasted children than the other two towns, which were not significantly different from each other. Significant differences were also observed between MUAC and sex of child where male children had higher MUAC than females. Wasting was significantly negatively associated with HDDS, with children from households with low HDDS tending to have large MUAC thus showing an inverse association among HDDS and obesity. However, further studies are needed to confirm this finding. In general food insecurity was closely associated with low wealth, food expenditure, large household size and limited access to land. The study did not find any significant role in the use of wild foods and social grants in improving food security for those households who were consuming wild foods and those receiving social grants. Although a greater percentage of households were food insecure, significant amounts of unprepared food (495±179 g per household) were wasted in Richards Bay alone in 48 hours mainly because the food had passed the best before date or had visibly gone bad. The amount of food waste was closely significantly negatively associated with household size. In general, the prevalence of food insecurity and wasting followed the agro-ecological gradient, with households in Richards Bay where the area.
- Full Text:
- Date Issued: 2017
- Authors: Chakona, Gamuchirai
- Date: 2017
- Subjects: Food security -- South Africa , Women -- South Africa -- Nutritiona , Urban women -- South Africa -- Nutrition , Rural women -- South Africa -- Nutrition , Wild foods -- South Africa , Food supply -- South Africa , Malnutrition in children -- South Africa , Grants-in-aid -- South Africa , Household ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/6484 , vital:21127
- Description: The lack of dietary diversity is a severe problem experienced by most poor households globally. Most poor communities are at high risk of inadequate intake of micronutrients resulting from diets dominated by starchy staples. The present study considered the diets, dietary diversities and food security of women of reproductive age between 15-49 years, households and communities along the rural-urban continuum in three mid-sized towns situated along an agro-ecological gradient in South Africa. A 48-hour dietary recall was performed across two seasons (twice in summer and once in winter) and focus group discussions were held to gather all information including food abundance, seasons of food scarcity as well as coping strategies which households employ during periods of food shocks. Households were further asked to quantify any type of food waste that they could have generated in the previous 48 hours. Nutritional status of children under the age of five in all three towns was also measured using height-for-age (HAZ) and mid-upper arm circumference (MUAC) as indicators of stunting and wasting respectively. The household surveys were conducted with 554 women randomly selected in rural, peri-urban and urban locations of Richards Bay, Dundee and Harrismith. For nutritional status, the sample consisted of 216 children who were randomly selected from the sampled households in rural, peri-urban and urban locations of the study sites. Household Dietary Diversity Scores (HDDS) and Women’s Dietary Diversity Scores (WDDS) were calculated from the food items consumed by each household and each woman over a two-day period, respectively. Household food access was also measured for each household using Household Food Insecurity Access Scale (HFIAS). The mean WDDS and HDDS for the wettest site of Richards Bay (3.8±0.29 and 8.44±1.72, respectively) was significantly higher than at Dundee (3.4±0.30 and 7.76±1.63, respectively) and Harrismith (3.5±0.27 and 7.83±1.59, respectively) which were not different from one another. The mean HFIAS for Dundee (9.39±7.13) was significantly higher than that in Richards Bay (5.57±6.98) and Harrismith (6.43±6.59) which were not significantly different from one another. Dietary diversity scores were also significantly higher in urban locations than in peri-urban and rural ones whilst HFIAS was significantly lower in the urban locations than peri-urban and rural locations. There was lower dependence on food purchasing in Richards Bay compared to Dundee and Harrismith where the majority of the population was purchasing most of their food. The majority of Richards Bay households were involved in subsistence agriculture and also produced a surplus for sale, as well as collecting wild foods, which improved food security, unlike Dundee and Harrismith. Food insecurity was higher in rural and peri-urban areas compared to urban areas. In all towns, food was always available throughout the year but was beyond the reach of many households. In urban areas food was readily available and only limited by access, whereas rural populations have limited access to affordable food and face higher prices. The peri-urban populations were more food insecure because of high levels of poverty, unemployment and lack of access/entitlements to land. Periurban dwellers are therefore more sensitive to changes in incomes and food prices because they lack safety nets to absorb income or price shocks as they purchase more, rather than growing their own food. Household dietary diversity was significantly negatively correlated with household food access, that is households with low HDDS had higher HFIAS scores. Due to high levels of food insecurity, a greater percentage of children under the age of five years were stunted (35 %) and wasted (18 %). There were no significant differences in stunting along the agro-ecological gradient, along the rural-urban continuum and sex of child. However, significant differences were observed in child wasting along the agro-ecological gradient with Harrismith having more wasted children than the other two towns, which were not significantly different from each other. Significant differences were also observed between MUAC and sex of child where male children had higher MUAC than females. Wasting was significantly negatively associated with HDDS, with children from households with low HDDS tending to have large MUAC thus showing an inverse association among HDDS and obesity. However, further studies are needed to confirm this finding. In general food insecurity was closely associated with low wealth, food expenditure, large household size and limited access to land. The study did not find any significant role in the use of wild foods and social grants in improving food security for those households who were consuming wild foods and those receiving social grants. Although a greater percentage of households were food insecure, significant amounts of unprepared food (495±179 g per household) were wasted in Richards Bay alone in 48 hours mainly because the food had passed the best before date or had visibly gone bad. The amount of food waste was closely significantly negatively associated with household size. In general, the prevalence of food insecurity and wasting followed the agro-ecological gradient, with households in Richards Bay where the area.
- Full Text:
- Date Issued: 2017
Characterisation, antimalarial and biological activities of secondary metabolites from leaves of anonidium mannii
- Authors: Makoni, Pfungwa Gervase
- Date: 2017
- Subjects: Anonidium mannii -- Therapeutic use , Botanical chemistry , Annonaceae -- Therapeutic use , Apocynaceae -- Therapeutic use , Malaria -- Chemotherapy , Tuberculosis -- Chemotherapy , Bacterial diseases -- Chemotherapy , Cancer -- Chemotherapy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4797 , vital:20725
- Description: Anonidium mannii is a plant of the Annonaceae genus which is used traditionally in Africa for the treatment of gonorrhoea, malaria, cancer, skin inflammation and dysentery. In this study we will evaluate antimalarial, antifungal, anti - tuberculosis, antibacterial activities and cytotoxicity of different fractions in order to provide a scientific rationale for the traditional use of Anonidium mannii as well as provide possible novel drugs in the treatment of multi drug resistant strains of parasites and bacteria. Extracts from dried leaves were obtained by using solvent extraction and different fractions obtained using column chromatography eluted with solvents of varying polarities to obtain a wide range of metabolites. The antimalarial activity of the various fractions and some pure compounds was evaluated using plasmodium lactate dehydrogenase (pLDH) assay. Cytotoxicity was evaluated using HeLa cells while anti – tuberculosis assay was evaluated using the green fluorescent protein. Antibacterial activity of the extracts was evaluated using micro-dilution assay against Gram-positive (Staphylococcus aureus and Enterococcus faecalis) bacteria and Gram-negative (Escherichia coli and Salmonella typhi) bacteria. Antifungal activity was evaluated against Candida albicans. The antimalarial assays yielded some fractions with promising IC50 values. The selected fractions yielded activities ranging between 0.73 μg/mL and 20.23 μg/mL. The fraction with the best activity was obtained from a hexane/ethyl acetate fraction. AM1C, a cholestane, showed the best activity from the pure metabolites that were screened. AM3C, stigmasterol, a pure compound gave the best antifungal activity with an MIC of 0.063 μg/mL. AM9C another pure compound (sterol) showed the best activity against S. typhi with a value of 0.031 μg/mL. AM2C a pure compound showed an activity of 0.063 μg/mL against E. faecalis. The best cytotoxicity was demonstrated by the fraction C2AM3P with a cell viability of 7.1 ± 0.2 % while AM1C had a viability of 20.2 ± 1.2 %. Several pure metabolites were isolated and four of these were positively identified as steroids. Of these steroids the structure of three novel metabolites from A. mannii was deduced. The study showed promising antibacterial, antifungal, anti – tuberculosis, antimalarial and anticancer activity of A. mannii. These results validate the use of A. manni against cancer, skin inflammation which is caused by fungus, malaria and bacterial diseases.
- Full Text:
- Date Issued: 2017
- Authors: Makoni, Pfungwa Gervase
- Date: 2017
- Subjects: Anonidium mannii -- Therapeutic use , Botanical chemistry , Annonaceae -- Therapeutic use , Apocynaceae -- Therapeutic use , Malaria -- Chemotherapy , Tuberculosis -- Chemotherapy , Bacterial diseases -- Chemotherapy , Cancer -- Chemotherapy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4797 , vital:20725
- Description: Anonidium mannii is a plant of the Annonaceae genus which is used traditionally in Africa for the treatment of gonorrhoea, malaria, cancer, skin inflammation and dysentery. In this study we will evaluate antimalarial, antifungal, anti - tuberculosis, antibacterial activities and cytotoxicity of different fractions in order to provide a scientific rationale for the traditional use of Anonidium mannii as well as provide possible novel drugs in the treatment of multi drug resistant strains of parasites and bacteria. Extracts from dried leaves were obtained by using solvent extraction and different fractions obtained using column chromatography eluted with solvents of varying polarities to obtain a wide range of metabolites. The antimalarial activity of the various fractions and some pure compounds was evaluated using plasmodium lactate dehydrogenase (pLDH) assay. Cytotoxicity was evaluated using HeLa cells while anti – tuberculosis assay was evaluated using the green fluorescent protein. Antibacterial activity of the extracts was evaluated using micro-dilution assay against Gram-positive (Staphylococcus aureus and Enterococcus faecalis) bacteria and Gram-negative (Escherichia coli and Salmonella typhi) bacteria. Antifungal activity was evaluated against Candida albicans. The antimalarial assays yielded some fractions with promising IC50 values. The selected fractions yielded activities ranging between 0.73 μg/mL and 20.23 μg/mL. The fraction with the best activity was obtained from a hexane/ethyl acetate fraction. AM1C, a cholestane, showed the best activity from the pure metabolites that were screened. AM3C, stigmasterol, a pure compound gave the best antifungal activity with an MIC of 0.063 μg/mL. AM9C another pure compound (sterol) showed the best activity against S. typhi with a value of 0.031 μg/mL. AM2C a pure compound showed an activity of 0.063 μg/mL against E. faecalis. The best cytotoxicity was demonstrated by the fraction C2AM3P with a cell viability of 7.1 ± 0.2 % while AM1C had a viability of 20.2 ± 1.2 %. Several pure metabolites were isolated and four of these were positively identified as steroids. Of these steroids the structure of three novel metabolites from A. mannii was deduced. The study showed promising antibacterial, antifungal, anti – tuberculosis, antimalarial and anticancer activity of A. mannii. These results validate the use of A. manni against cancer, skin inflammation which is caused by fungus, malaria and bacterial diseases.
- Full Text:
- Date Issued: 2017
Characterization of the distribution of platinum group elements in sulphide ores within the Merensky Reef at Modikwa and Two Rivers Platinum Mines, Eastern Bushveld Complex, South Africa
- Authors: Zilibokwe, Nosibulelo Julie
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/7938 , vital:21326
- Description: The distribution of the platinum group element (PGE), in the Merensky Reef was characterized by, first determining the occurrence of the platinum group minerals (PGM), then by establishing the PGE concentration in the base metal sulphides (BMS) associated with the PGE mineralization in the Merensky Reef from selected borehole intersections, at the Two Rivers (TRP) and Modikwa Platinum Mines in the Eastern Bushveld Complex. A mineral liberation analyser (MLA) was then used to identify the PGM phases; their silicate and base metal associations; and their grain size distribution. Electron microprobe quantitative analysis and mapping were then used to determine the compositional variation of the PGM and the PGE elemental distribution in the BMS, respectively. The study showed that the BMS including pyrrhotite, pentlandite, and chalcopyrite were the principal sulphides, where pyrrhotite was most prominent with minor quantities of pyrite. Orthopyroxene, clinopyroxene and plagioclase were the most abundant primary silicate minerals identified, while secondary silicates identified included talc, serpentine and amphibole. Platinum group minerals showed three distinct groups with respect to the mineralogical association with the PGE; (i) BMS association; (ii) chromite association; and (iii) silicate association. Of the BMS, chalcopyrite showed the most dominant association with the PGMs. All samples from both mines exhibited a wide range of PGMs, including maslovite, braggite, cooperate, laurite and PGE alloys such as ferroplatinum as well as other unidentified platinum and palladium sulphides, arsenides and bismuthides, while gold was present as electrum. The PGMs ranged in size from less than a micron to about 125 microns with an average of 20 microns. The close association of PGM with BMS along the margins of sulphides indicates that the PGMs were derived from the sulphide melt. PGE distribution in the sulphides at Modikwa showed pentlandite contained the highest concentrations of palladium (up to 379 ppm) and chalcopyrite hosting the highest rhodium concentrations (up to 793 ppm). Samples from Two Rivers revealed pentlandite as the principal host to both palladium and rhodium, with concentrations reaching up to 695 and 930 ppm, respectively. Magnetite at both Modikwa and Two Rivers showed significant rhodium content, reaching up to 982 and 930 ppm, respectively. The pyrrhotite compared to other sulphides contained all the elements found in the platinum group (PPGE), namely, platinum, palladium and rhodium, with all the platinum identified found in the pyrrhotite. The concentrations for the iridium group (IPGE) namely, iridium, osmium, and ruthenium were below the detection limit. The PGE mineralization in the stratigraphy varied within each mine. The mineralization revealed top loading in the central sector (Modikwa) and bottom loading in the southern sector (Two Rivers). The sequence of the Merensky Reef at the two sectors of the Eastern Bushveld Complex showed a remarkable similarity in their mineralogy suggesting that these two sectors were formed from the same liquid or formed simultaneously within a single magma chamber; however the PGE distribution within the stratigraphy may have been controlled by the presence of cumulate sulphides.
- Full Text:
- Date Issued: 2017
- Authors: Zilibokwe, Nosibulelo Julie
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/7938 , vital:21326
- Description: The distribution of the platinum group element (PGE), in the Merensky Reef was characterized by, first determining the occurrence of the platinum group minerals (PGM), then by establishing the PGE concentration in the base metal sulphides (BMS) associated with the PGE mineralization in the Merensky Reef from selected borehole intersections, at the Two Rivers (TRP) and Modikwa Platinum Mines in the Eastern Bushveld Complex. A mineral liberation analyser (MLA) was then used to identify the PGM phases; their silicate and base metal associations; and their grain size distribution. Electron microprobe quantitative analysis and mapping were then used to determine the compositional variation of the PGM and the PGE elemental distribution in the BMS, respectively. The study showed that the BMS including pyrrhotite, pentlandite, and chalcopyrite were the principal sulphides, where pyrrhotite was most prominent with minor quantities of pyrite. Orthopyroxene, clinopyroxene and plagioclase were the most abundant primary silicate minerals identified, while secondary silicates identified included talc, serpentine and amphibole. Platinum group minerals showed three distinct groups with respect to the mineralogical association with the PGE; (i) BMS association; (ii) chromite association; and (iii) silicate association. Of the BMS, chalcopyrite showed the most dominant association with the PGMs. All samples from both mines exhibited a wide range of PGMs, including maslovite, braggite, cooperate, laurite and PGE alloys such as ferroplatinum as well as other unidentified platinum and palladium sulphides, arsenides and bismuthides, while gold was present as electrum. The PGMs ranged in size from less than a micron to about 125 microns with an average of 20 microns. The close association of PGM with BMS along the margins of sulphides indicates that the PGMs were derived from the sulphide melt. PGE distribution in the sulphides at Modikwa showed pentlandite contained the highest concentrations of palladium (up to 379 ppm) and chalcopyrite hosting the highest rhodium concentrations (up to 793 ppm). Samples from Two Rivers revealed pentlandite as the principal host to both palladium and rhodium, with concentrations reaching up to 695 and 930 ppm, respectively. Magnetite at both Modikwa and Two Rivers showed significant rhodium content, reaching up to 982 and 930 ppm, respectively. The pyrrhotite compared to other sulphides contained all the elements found in the platinum group (PPGE), namely, platinum, palladium and rhodium, with all the platinum identified found in the pyrrhotite. The concentrations for the iridium group (IPGE) namely, iridium, osmium, and ruthenium were below the detection limit. The PGE mineralization in the stratigraphy varied within each mine. The mineralization revealed top loading in the central sector (Modikwa) and bottom loading in the southern sector (Two Rivers). The sequence of the Merensky Reef at the two sectors of the Eastern Bushveld Complex showed a remarkable similarity in their mineralogy suggesting that these two sectors were formed from the same liquid or formed simultaneously within a single magma chamber; however the PGE distribution within the stratigraphy may have been controlled by the presence of cumulate sulphides.
- Full Text:
- Date Issued: 2017
Characterization of the Mitochondrial Plasmodium falciparum heat shock protein 70
- Authors: Nyakundi, David Onchong’a
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/44449 , vital:25408 , https://doi.org/10.21504/10962/44448
- Description: Malaria remains a global health problem and accounts for many deaths and illnesses in subSaharan Africa. Plasmodium falciparum, the causative agent of the most fatal form of malaria, expresses a repertoire of heat shock proteins for cytoprotection, survival and pathogenesis. The parasite genome encodes six Hsp70 proteins found in various cell compartments. However, the putative parasite mitochondrial Hsp70 (PfHsp70-3) has not been investigated. The J-proteins, Pfj1 and PFF1415c, were proposed to function as co-chaperones of PfHsp70-3. The biochemical characterization of PfHsp70-3 was initially complicated by the fact that the protein was insoluble when expressed in E. coli cells. Various approaches to solubilize it resulted in inactive protein. A general characteristic of eukaryotic mitochondrial Hsp70s is their insolubility and their reliance on an Hsp70 escort protein (Hep) for solubility and ultimate functions. In this study, a putative Hep protein was identified in the genome of P. falciparum that is referred to as PfHep1. Coexpression of PfHep1 with PfHsp70-3 resulted in soluble and biochemically active PfHsp70-3. Size exclusion chromatography was employed to separate PfHsp70-3 from PfHep1 after coexpression. PfHep1 suppressed thermally induced aggregation of PfHsp70-3 but not the aggregation of malate dehydrogenase or citrate synthase, thus showing specificity for PfHsp70-3. Zinc ions were also found to be essential for maintaining the functions of PfHep1, as EDTA chelation abrogated its abilities to suppress the aggregation of PfHsp70-3. Furthermore, PfHep1 did not stimulate the basal ATPase or increase refoldase activities of PfHsp70-3 hence displaying no co-chaperone roles. The full-length putative mitochondrial type I J protein, Pfj1, could not be produced in E.coli but a truncated protein containing the J-domain was produced which stimulated both the ATPase and refoldase activities of PfHsp70-3. Further, this study demonstrated that both PfHep1 and PfHsp70-3 localized to the mitochondrion in the erythrocytic stage of P. falciparum development thus confirming in silico predictions of their localization. Besides, PfHsp70-3 was expressed during all stages of the intraerythrocytic cycle of parasite development and was heat inducible. Generally, the data obtained in this study will enhance the existing knowledge on the biology of the parasite mitochondrial chaperone functions and open the possible avenue of drug targeting considering the specificity of PfHsp70- 3 and PfHep1 partnerships.
- Full Text:
- Date Issued: 2017
- Authors: Nyakundi, David Onchong’a
- Date: 2017
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/44449 , vital:25408 , https://doi.org/10.21504/10962/44448
- Description: Malaria remains a global health problem and accounts for many deaths and illnesses in subSaharan Africa. Plasmodium falciparum, the causative agent of the most fatal form of malaria, expresses a repertoire of heat shock proteins for cytoprotection, survival and pathogenesis. The parasite genome encodes six Hsp70 proteins found in various cell compartments. However, the putative parasite mitochondrial Hsp70 (PfHsp70-3) has not been investigated. The J-proteins, Pfj1 and PFF1415c, were proposed to function as co-chaperones of PfHsp70-3. The biochemical characterization of PfHsp70-3 was initially complicated by the fact that the protein was insoluble when expressed in E. coli cells. Various approaches to solubilize it resulted in inactive protein. A general characteristic of eukaryotic mitochondrial Hsp70s is their insolubility and their reliance on an Hsp70 escort protein (Hep) for solubility and ultimate functions. In this study, a putative Hep protein was identified in the genome of P. falciparum that is referred to as PfHep1. Coexpression of PfHep1 with PfHsp70-3 resulted in soluble and biochemically active PfHsp70-3. Size exclusion chromatography was employed to separate PfHsp70-3 from PfHep1 after coexpression. PfHep1 suppressed thermally induced aggregation of PfHsp70-3 but not the aggregation of malate dehydrogenase or citrate synthase, thus showing specificity for PfHsp70-3. Zinc ions were also found to be essential for maintaining the functions of PfHep1, as EDTA chelation abrogated its abilities to suppress the aggregation of PfHsp70-3. Furthermore, PfHep1 did not stimulate the basal ATPase or increase refoldase activities of PfHsp70-3 hence displaying no co-chaperone roles. The full-length putative mitochondrial type I J protein, Pfj1, could not be produced in E.coli but a truncated protein containing the J-domain was produced which stimulated both the ATPase and refoldase activities of PfHsp70-3. Further, this study demonstrated that both PfHep1 and PfHsp70-3 localized to the mitochondrion in the erythrocytic stage of P. falciparum development thus confirming in silico predictions of their localization. Besides, PfHsp70-3 was expressed during all stages of the intraerythrocytic cycle of parasite development and was heat inducible. Generally, the data obtained in this study will enhance the existing knowledge on the biology of the parasite mitochondrial chaperone functions and open the possible avenue of drug targeting considering the specificity of PfHsp70- 3 and PfHep1 partnerships.
- Full Text:
- Date Issued: 2017
Chemical composition of leaf essential oils of Lantana camara varieties in South Africa and their effect on the behavioural preference of Falconia intermedia
- Authors: Ngxande-Koza, Samella W
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4877 , vital:20739
- Description: Lantana camara L. (Verbenaceae) is one the most problematic invaders in South Africa invading forest edges, sand dunes, and shorelines by forming impenetrable thickets. Lantana camara invasions degrade natural biodiversity, reduce the value of land and consequently it has been a target for biological control, over the last 50 years in South Africa. Studies that have reported on chemical profile of Lantana camara have been conducted around the world but not in South Africa. Hence, the first aim of the current study was to identify the chemical baseline of L. camara varieties in the Eastern Cape, South Africa. Recent studies have shown that feeding by one of the agents released against L. camara, Falconia intermedia (Distant) (Hemiptera: Miridae), induces anti-herbivory response through increased leaf toughness and trichome density. A preliminary study conducted also reported the production of volatile chemicals by one variety, Whitney Farm, due to feeding by the mirids. Therefore, the second aim was to determine the induced changes in chemical compounds of L. camara varieties after feeding by F. intermedia. A third aim was to determine the effect these chemical compounds have on the behaviour of F. intermedia. To identify the chemical baseline of L. camara varieties, the essential oils of four L. camara varieties (East London, Port Alfred, Whitney Farm and Heather Glen) were analysed using gas chromatography mass spectrometry (GC-MS) and that resulted to the identification of 163 constitutive and 75 induced chemicals across the varieties tested. Lantana camara varieties showed different chemical classes but were highly dominated by terpenes. A great variation in the number of constitutive chemical compounds was found in all the varieties. There were 56 constitutive chemical compounds in the Whitney Farm variety, 41 in the East London variety, 36 in the Heather Glen variety and 30 in the Port Alfred variety. The Whitney Farm variety had the highest number (22) of unique constitutive chemicals identified when compared with other varieties. This indicates the chemical distinctiveness of the Whitney Farm variety from the other varieties. In the varieties tested, there were common chemical compounds identified in constitutive and induced (discussed below) states of the plants such as caryophyllene, hexane, naphthalene, copaene and a-caryophyllene. Besides naphthalene, the majority of chemical compounds in South African L. camara varieties were similar to compounds that have been identified across the world, suggesting that they are closely related. The expression of naphthalene in these varieties may be due to changes in the chemicals expressed over evolutionary time as predicted by the Novel Weapons Hypothesis. Amongst the varieties, a great variation in chemical compounds and their concentrations was shown in the induced states of the plants. The concentration of constitutive caryophyllene ranged from (3.13 - 15.7) %, to (4.02 - 11.10) % after feeding. The concentration of constitutive hexane ranged from (6.13 - 71.19) %, to (33.3 - 75.8) % after feeding. The concentration of constitutive naphthalene ranged from (0.21 - 4.79) %, to (0.92 - 2.11) % after feeding. The concentration of constitutive copaene ranged from (0.57 - 1.57) %, to (1.20 - 2.72) %. Lastly, the concentration of constitutive a-caryophyllene ranged from (1.18 - 9.03) %, to (0.78 - 5.48) % after feeding. The changes in chemical concentrations in lantana varieties indicated that feeding by the mirid on L. camara varieties causes an induction by either reducing or increasing the chemical concentrations. To determine the effect of the identified compounds on the behaviour of F. intermedia adults, olfactometer bioassays were conducted using a Y-tube technique. A significantly higher proportion of F. intermedia were attracted to undamaged leaves over damaged leaves and purified air. Undamaged leaves attracted 52 % of F. intermedia from the East London variety, 62.5 % from the Port Alfred variety, 56 % from the Whitney Farm variety, 58 % from the Lyndhurst variety and 54.5 % from the Heather Glen variety in dual choice trials versus damaged leaves. Furthermore, a significantly higher proportion of F. intermedia were attracted to damaged leaves over purified air. Damaged leaves attracted 67 % of F. intermedia from the East London variety, 67 % from the Port Alfred variety, 65.9 % from the Whitney Farm variety, 65.3 % from the Heather Glen variety and 64.5 % from the Lyndhurst variety. Olfactometer bioassays were also conducted using purified standard compounds of four chemical compounds identified from essential oils, hexane was used as a positive control as it is reported to be an insect attractant in literature. Hexane was highly attractive to the mirids compared to three standard compounds caryophyllene, caryophyllene oxide and naphthalene at the rate of 80 %, 73 % and 80 %, respectively. The standard compounds tested against F. intermedia are major compounds contained by L. camara varieties and they have proven to have a repellent effect. This may indicate that after feeding by F. intermedia, the major compounds expressed by the plant varieties repel F. intermedia contributing to the invasiveness of this weed. The increased expression of hexane and caryophyllene after feeding may also indicate increased attraction to some insects, opening up the potential for third trophic level interactions in varieties where this is the case. This is the first study on the chemical composition of essential oils of L. camara in South Africa. Therefore, we recommend that where appropriate chemical profile studies of the invasive alien plants should be considered during host specificity testing, and the vital role of chemical compounds on agent-weed interactions must be taken into consideration with other factors before and after the biological control agents are released.
- Full Text:
- Date Issued: 2017
- Authors: Ngxande-Koza, Samella W
- Date: 2017
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/4877 , vital:20739
- Description: Lantana camara L. (Verbenaceae) is one the most problematic invaders in South Africa invading forest edges, sand dunes, and shorelines by forming impenetrable thickets. Lantana camara invasions degrade natural biodiversity, reduce the value of land and consequently it has been a target for biological control, over the last 50 years in South Africa. Studies that have reported on chemical profile of Lantana camara have been conducted around the world but not in South Africa. Hence, the first aim of the current study was to identify the chemical baseline of L. camara varieties in the Eastern Cape, South Africa. Recent studies have shown that feeding by one of the agents released against L. camara, Falconia intermedia (Distant) (Hemiptera: Miridae), induces anti-herbivory response through increased leaf toughness and trichome density. A preliminary study conducted also reported the production of volatile chemicals by one variety, Whitney Farm, due to feeding by the mirids. Therefore, the second aim was to determine the induced changes in chemical compounds of L. camara varieties after feeding by F. intermedia. A third aim was to determine the effect these chemical compounds have on the behaviour of F. intermedia. To identify the chemical baseline of L. camara varieties, the essential oils of four L. camara varieties (East London, Port Alfred, Whitney Farm and Heather Glen) were analysed using gas chromatography mass spectrometry (GC-MS) and that resulted to the identification of 163 constitutive and 75 induced chemicals across the varieties tested. Lantana camara varieties showed different chemical classes but were highly dominated by terpenes. A great variation in the number of constitutive chemical compounds was found in all the varieties. There were 56 constitutive chemical compounds in the Whitney Farm variety, 41 in the East London variety, 36 in the Heather Glen variety and 30 in the Port Alfred variety. The Whitney Farm variety had the highest number (22) of unique constitutive chemicals identified when compared with other varieties. This indicates the chemical distinctiveness of the Whitney Farm variety from the other varieties. In the varieties tested, there were common chemical compounds identified in constitutive and induced (discussed below) states of the plants such as caryophyllene, hexane, naphthalene, copaene and a-caryophyllene. Besides naphthalene, the majority of chemical compounds in South African L. camara varieties were similar to compounds that have been identified across the world, suggesting that they are closely related. The expression of naphthalene in these varieties may be due to changes in the chemicals expressed over evolutionary time as predicted by the Novel Weapons Hypothesis. Amongst the varieties, a great variation in chemical compounds and their concentrations was shown in the induced states of the plants. The concentration of constitutive caryophyllene ranged from (3.13 - 15.7) %, to (4.02 - 11.10) % after feeding. The concentration of constitutive hexane ranged from (6.13 - 71.19) %, to (33.3 - 75.8) % after feeding. The concentration of constitutive naphthalene ranged from (0.21 - 4.79) %, to (0.92 - 2.11) % after feeding. The concentration of constitutive copaene ranged from (0.57 - 1.57) %, to (1.20 - 2.72) %. Lastly, the concentration of constitutive a-caryophyllene ranged from (1.18 - 9.03) %, to (0.78 - 5.48) % after feeding. The changes in chemical concentrations in lantana varieties indicated that feeding by the mirid on L. camara varieties causes an induction by either reducing or increasing the chemical concentrations. To determine the effect of the identified compounds on the behaviour of F. intermedia adults, olfactometer bioassays were conducted using a Y-tube technique. A significantly higher proportion of F. intermedia were attracted to undamaged leaves over damaged leaves and purified air. Undamaged leaves attracted 52 % of F. intermedia from the East London variety, 62.5 % from the Port Alfred variety, 56 % from the Whitney Farm variety, 58 % from the Lyndhurst variety and 54.5 % from the Heather Glen variety in dual choice trials versus damaged leaves. Furthermore, a significantly higher proportion of F. intermedia were attracted to damaged leaves over purified air. Damaged leaves attracted 67 % of F. intermedia from the East London variety, 67 % from the Port Alfred variety, 65.9 % from the Whitney Farm variety, 65.3 % from the Heather Glen variety and 64.5 % from the Lyndhurst variety. Olfactometer bioassays were also conducted using purified standard compounds of four chemical compounds identified from essential oils, hexane was used as a positive control as it is reported to be an insect attractant in literature. Hexane was highly attractive to the mirids compared to three standard compounds caryophyllene, caryophyllene oxide and naphthalene at the rate of 80 %, 73 % and 80 %, respectively. The standard compounds tested against F. intermedia are major compounds contained by L. camara varieties and they have proven to have a repellent effect. This may indicate that after feeding by F. intermedia, the major compounds expressed by the plant varieties repel F. intermedia contributing to the invasiveness of this weed. The increased expression of hexane and caryophyllene after feeding may also indicate increased attraction to some insects, opening up the potential for third trophic level interactions in varieties where this is the case. This is the first study on the chemical composition of essential oils of L. camara in South Africa. Therefore, we recommend that where appropriate chemical profile studies of the invasive alien plants should be considered during host specificity testing, and the vital role of chemical compounds on agent-weed interactions must be taken into consideration with other factors before and after the biological control agents are released.
- Full Text:
- Date Issued: 2017