Stress-inducible protein 1: a bioinformatic analysis of the human, mouse and yeast STI1 gene structure
- Authors: Aken, Bronwen Louise
- Date: 2005
- Subjects: Molecular chaperones , Proteins -- Analysis , Heat shock proteins , Bioinformatics , Genetics -- Data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3990 , http://hdl.handle.net/10962/d1004049 , Molecular chaperones , Proteins -- Analysis , Heat shock proteins , Bioinformatics , Genetics -- Data processing
- Description: Stress-inducible protein 1 (Sti1) is a 60 kDa eukaryotic protein that is important under stress and non-stress conditions. Human Sti1 is also known as the Hsp70/Hsp90 organising protein (Hop) that coordinates the functional cooperation of heat shock protein 70 (Hsp70) and heat shock protein 90 (Hsp90) during the folding of various transcription factors and kinases, including certain oncogenic proteins and prion proteins. Limited studies have been conducted on the STI1 gene structure. Thus, the aim of this study was to develop a comprehensive description of human STI1 (hSTI1), mouse STI1 (mSTI1), and yeast STI1 (ySTI1) genes, using a bioinformatic approach. Genes encoded near the STI1 loci were identified for the three organisms using National Centre for Biotechnology Information (NCBI) MapViewer and the Saccharomyces Genome Database. Exon/intron boundaries were predicted using Hidden Markov model gene prediction software (HMMGene) and Genscan, and by alignment of the mRNA sequence with the genomic DNA sequence. Transcription factor binding sites (TFBS) were predicted by scanning the region 1000 base pairs (bp) upstream of the STI1 orthologues’ transcription start site (TSS) with Alibaba, Transcription element search software (TESS) and Transcription factor search (TFSearch). The promoter region was defined by comparing the number, type and position of TFBS across the orthologous STI1 genes. Additional putative TFBS were identified for ySTI1 by searching with software that aligns nucleic acid conserved elements (AlignACE) for over-represented motifs in the region upstream of the TSS of genes thought to be co-regulated with ySTI1. This study showed that hSTI1 and mSTI1 occur in a region of synteny with a number of genes of related function. Both hSTI1 and mSTI1 comprised 14 putative exons, while ySTI1 was encoded on a single exon. Human and mouse STI1 shared a perfectly conserved 55 bp region spanning their predicted TSS, although their TATA boxes were not conserved. A putative CpG island was identified in the region from -500 to +100 bp relative to the hSTI1 and mSTI1 TSS. This region overlapped with a region of high TFBS density, suggesting that the core promoter region was located in the region approximately 100 to 200 bp upstream of the TSS. Several conserved clusters of TFBS were also identified upstream of this promoter region, including binding sites for stimulatory protein 1 (Sp1), heat shock factor (HSF), nuclear factor kappa B (NF-kappaB), and the cAMP/enhancer binding protein (C/EBP). Microarray data suggested that ySTI1 was co-regulated with several heat shock proteins and substrates of the Hsp70/Hsp90 heterocomplex, and several putative regulatory elements were identified in the upstream region of these co-regulated genes, including a motif for HSF binding. The results of this research suggest several avenues of future experimental work, including the confirmation of the proposed core promoter, upstream regulatory elements, and CpG island, and the investigation into the co-regulation of mammalian STI1 with its surrounding genes. These results could also be used to inform STI1 gene knockout experiments in mice, to assess the biological importance of mammalian STI1.
- Full Text:
- Date Issued: 2005
The role of parallel computing in bioinformatics
- Authors: Akhurst, Timothy John
- Date: 2005
- Subjects: Bioinformatics , Parallel programming (Computer science) , LINDA (Computer system) , Java (Computer program language) , Parallel processing (Electronic computers) , Genomics -- Data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3986 , http://hdl.handle.net/10962/d1004045 , Bioinformatics , Parallel programming (Computer science) , LINDA (Computer system) , Java (Computer program language) , Parallel processing (Electronic computers) , Genomics -- Data processing
- Description: The need to intelligibly capture, manage and analyse the ever-increasing amount of publicly available genomic data is one of the challenges facing bioinformaticians today. Such analyses are in fact impractical using uniprocessor machines, which has led to an increasing reliance on clusters of commodity-priced computers. An existing network of cheap, commodity PCs was utilised as a single computational resource for parallel computing. The performance of the cluster was investigated using a whole genome-scanning program written in the Java programming language. The TSpaces framework, based on the Linda parallel programming model, was used to parallelise the application. Maximum speedup was achieved at between 30 and 50 processors, depending on the size of the genome being scanned. Together with this, the associated significant reductions in wall-clock time suggest that both parallel computing and Java have a significant role to play in the field of bioinformatics.
- Full Text:
- Date Issued: 2005
Euphausiid population structure and grazing in the Indian sector of the Antarctic Polar Frontal Zone, during austral autumn
- Authors: Bernard, Anthony Thomas Firth
- Date: 2005
- Subjects: Euphausiacea -- Antarctic Ocean , Euphausiacea -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5913 , http://hdl.handle.net/10962/d1015960
- Description: The trophodynamics of the numerically dominant euphausiid species within a region of high mesoscale oceanographic variability in the southwest Indian sector of the Antarctic Polar Frontal Zone (PFZ) were investigated during the austral autumns April/May) of 2004 and 2005. During the 2004 survey, sub-surface (200 m) temperature profiles indicated that an intense frontal feature, formed by the convergence of the Sub-Antarctic Front (SAF) and the Antarctic Polar Front (APF) bisected the survey area into two distinct zones, the Sub- Antarctic Zone (SAZ) and the Antarctic Zone (AAZ). Total integrated chlorophyll-a (chl-a) biomass was typical for the region (< 25 mg chl-a m⁻²), and was dominated by picophytoplankton. Total euphausiid abundance and biomass ranged from 0.1 to 3.1 ind m⁻³ and 0.1 to 8.1 mg dwt m⁻³, respectively, and did not differ significantly between the stations occupied in the SAZ and AAZ (p > 0.05). The multivariate analysis identified two interacting mechanisms controlling the distribution patterns, abundance and biomass of the various euphausiid species, namely (1) diel changes in abundance and biomass, (2) and restricted distribution patterns associated with the different water masses. Ingestion rates were determined for five euphausiid species. E. triacantha was found to have the highest daily ingestion rate ranging from 1 226.1 to 6 029.1 ng pigm ind⁻¹d⁻¹, while the lowest daily ingestion rates were observed in the juvenile Thysanoessa species (6.4 to 943.0 ng pigm ind⁻¹ d⁻¹). The total grazing impact of the selected euphausiids ranged from < 0.1 to 20.1 μg pigm m⁻²d⁻¹, corresponding to < 0.15 % of the areal chl-a biomass. The daily ration estimates of autotrophic carbon for the euphausiids suggested that phytoplankton represented a minor component in their diets, with only the sub-adult E. vallentini consuming sufficient phytoplankton to meet their daily carbon requirements. A cyclonic cold-core eddy spawned from the region of the APF located in the southwest Indian sector of the PFZ was the dominant feature during the 2005 survey. The total areal chl-a biomass throughout the region was low, ranging between 5.6 and 11.4 mg chl-a m⁻², and was significantly higher within the core of the eddy compared to the surrounding waters (p < 0.05). RMT-8 and WP-2 total euphausiid abundance and biomass estimates were high, and ranged from 0.004 to 0.36 ind m⁻³ and 0.065 to 1.21 mg dwt m⁻³, and from 0.01 to 18.2 ind m⁻³ and 0.01 to 15.7 mg dwt m⁻³, respectively. A distinct spatial pattern in the euphausiid community was evident with the Antarctic species, Euphausia frigida, E. triacantha and E. superba predominating within the core of the eddy, while the PFZ waters were characterized by the sub-Antarctic species, E. longirostris, Stylocheiron maximum, Nematoscelis megalops and Thysanoessa gregaria. The eddy edge acted as a transition zone where species from both regions co-occurred. Within the survey area the combined ingestion rate of the six numerically dominant euphausiid species ranged between 0.02 and 5.31 μg pigm m⁻²d¹, which corresponded to a loss of between < 0.001 and 0.11 % of the available chl-a biomass. E. triacantha and juvenile T. macura were identified as the dominant grazers. There was no apparent spatial pattern in the grazing activity of the euphausiids within the region of investigation. The average daily rations of the euphausiids examined were < 2 % of their body carbon. The low daily ration of the euphausiids could be ascribed to the predominance of small picophytoplankton in the region of investigation, which are too small to be grazed efficiently by larger zooplankton. The marked spatial patterns in species composition and the elevated abundance and biomass of euphausiids, suggest that the mesoscale eddies contribute to the spatial and temporal heterogeneity of the planktonic community of the PFZ and may represent important foraging regions for many of the apex predators within the region.
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- Date Issued: 2005
The feeding ecology, habitat selection and hunting behaviour of re-introduced cheetah on Kwandwe Private Game Reserve, Eastern Cape Province
- Authors: Bissett, Charlene
- Date: 2005
- Subjects: Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5698 , http://hdl.handle.net/10962/d1005384 , Kwandwe Game Reserve (South Africa) , Cheetah -- Behavior -- South Africa -- Eastern Cape , Cheetah -- Nutrition -- South Africa -- Eastern Cape , Cheetah -- South Africa -- Eastern Cape
- Description: The re-introduction of cheetah onto small reserves in the Eastern Cape Province has created the opportunity to study this species in an environment (Valley Bushveld) in which it has not previously been studied and which is quite different from the less wooded habitats in which previous studies have occurred. Thus the aim of this study is to add to the growing number of studies of cheetah in more wooded habitats and to contribute a better understanding of the level of specialization or adaptability in space use, habitat selection, diet and hunting behaviour that the cheetah possesses. The research was conducted on Kwandwe Private Game Reserve from February 2003 to August 2004. Direct observations, scat analysis and continuous observations were used to avoid bias towards large sized prey found when only using direct observations to study diet. The cheetah killed 15 species and four of the five most important prey species (kudu, springbok, grey duiker and bushbuck) were hunted according to their abundance on the reserve, while impala, the fifth most important prey species, was avoided (i.e. preference index less than one) by the cheetah. The cheetah’s main prey was medium sized ungulates, although the three male coalition killed larger prey than females and females with cubs. The daily food intake per cheetah per day was calculated to range from 5.3kg/cheetah/day for the coalition to 8kg/cheetah/day for a solitary female. Home range areas ranged from 11.1 km² for female cheetah with cubs in a den to 65.6 km² for single female cheetah and core areas ranged from 6 km² for the three male coalition to 26.5 km² for independent cubs. Habitat selection by cheetah on Kwandwe varied between the social groups depending on their susceptibility to predation by lions, their need for cover and need for water, and was similar to what has been previously reported. The home range of the coalition incorporated the most open vegetation type (karroid shrubland) with surrounding denser vegetation, while females occupied areas of denser vegetation. Activity patterns and hunting behaviour varied between different cheetah social groups with female cheetah being more active during day light hours compared to males which made 38% of their kills after dark. The average chase distances for the various cheetah groups varied considerably, and the chase distance for successful hunts was longer than for unsuccessful for all groups except single female cheetah. The percentage of kills’ kleptoparasitised on Kwandwe was very low compared to other studies possibly due to the low density of direct competitors, which in turn lead to longer mean kill retention times. These results suggest that cheetah are more adaptable than previously thought and this adaptability may have important implications for their conservation.
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- Date Issued: 2005
The biology of suburban olive thrushes (Turdus olivaceus olivaceus) in the Eastern Cape, South Africa
- Authors: Bonnevie, Bo Tørris
- Date: 2005
- Subjects: Thrushes , Turdus , Thrushes -- South Africa -- Eastern Cape , Turdus -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5651 , http://hdl.handle.net/10962/d1005333 , Thrushes , Turdus , Thrushes -- South Africa -- Eastern Cape , Turdus -- South Africa -- Eastern Cape
- Description: This study investigated the biology of the Olive Thrush Turdus olivaceus olivaceus in Grahamstown, South Africa from 1998 to 2003. Behavioural differences between males and females, parent-young interactions, development of fledglings, and the role of song and moult in territorial behaviour were investigated. Comparisons of biometrics, moult and survival were made using Olive Thrush ringing data from other regions. There were no significant differences in mass, wing length or survival rate between the Eastern and Western Cape provinces, but primary wing moult started earlier in the Western Cape and coincided with the end of the main breeding periods in both provinces. Both males and females of breeding pairs remained in and defended their territories throughout the year, but there was some evidence that territorial defence was strongest during the breeding periods. Roughly, every 100 eggs laid produce 50 fledglings. Out of these 20 to 30 reach the age of independence at approximately 50 days, and only five of these juvenile birds reach maturity. Adult survival was estimated at 80%, with a mean life expectancy of 4.5 years and a conservative estimate of maximum lifespan of 11 years. Using ringing data and museum specimens, the Olive Thrush was compared with the Karoo Thrush Turdus smithi, a former race of the Olive Thrush. Olive Thrushes had shorter bills and wings, but were heavier than Karoo Thrushes from the Cradock district. There were also differences in bill and eye-ring colouration between these populations. No morphological differences were found between the sexes in either species.
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- Date Issued: 2005
The role of acute toxicity data for South African freshwater macroinvertebrates in the derivation of water quality guidelines for salinity
- Authors: Browne, Samantha
- Date: 2005
- Subjects: Water-supply -- South Africa , Water quality management -- South Africa , Aquatic ecology -- South Africa , Ecosystem management -- South Africa , Freshwater invertebrates -- South Africa -- Ecology , Water -- Toxicology -- South Africa , Water quality biological assessment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4742 , http://hdl.handle.net/10962/d1006173 , Water-supply -- South Africa , Water quality management -- South Africa , Aquatic ecology -- South Africa , Ecosystem management -- South Africa , Freshwater invertebrates -- South Africa -- Ecology , Water -- Toxicology -- South Africa , Water quality biological assessment
- Description: Water resources are under ever-increasing pressure to meet the demands of various water users both nationally and internationally. The process of anthropogenically-induced salinisation serves to exacerbate this pressure by limiting the quantity and quality of water available for future use. Water quality guidelines provide the numerical goals which water resource managers can use to adequately manage and protect aquatic ecosystems. Various methods which have been developed and used internationally to derive such guidelines are discussed. Acute toxicity tests were conducted using two inorganic salts, NaCl and Na₂SO₄. Field collected, indigenous, freshwater macroinvertebrates were used as tests organisms. Data generated from these tests contributed to the expansion of the currently limited toxicological database of response data for indigenous organisms and the suitability of using such organisms for future testing was discussed. Salt sensitivities of indigenous freshwater invertebrates were compared those of species sourced from an international toxicological database and were found to have similar ranges of tolerances to NaCl and Na₂SO₄. Species sensitivity distributions (SSDs), a method of data extrapolation, were derived using different types of toxicological data, and hence different guideline values or protective concentrations were derived. These concentrations were equated to boundary values for South Africa’s ecological Reserve categories, which are used to describe degrees of health for aquatic ecosystems. Provisional results suggest that using only acute toxicity data in guideline derivation provides ecosystem protection that is under-protective. Chronic toxicity data, which include endpoints other than mortality, provide the most realistic environmental protection but lack data confidence due to small sample sizes (acute tests are more readily conducted than chronic tests). The potential contribution of sub-chronic data to guideline derivation is highlighted as these data are more readily extrapolated to chronic endpoints than acute data and sub-chronic tests are not as complex and demanding to conduct as chronic tests.
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- Date Issued: 2005
A floristic study of a portion of the Pondoland Centre of Endeminism, Port St Johns, South Africa
- Authors: Cloete, Elizabeth Carinus
- Date: 2005
- Subjects: Endemic plants -- South Africa -- Port St Johns , Phytogeography -- South Africa -- Port St Johns , Plants -- South Africa -- Port St Johns , Alien plants -- South Africa -- Port St Johns
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4188 , http://hdl.handle.net/10962/d1003757 , Endemic plants -- South Africa -- Port St Johns , Phytogeography -- South Africa -- Port St Johns , Plants -- South Africa -- Port St Johns , Alien plants -- South Africa -- Port St Johns
- Description: Analysis of the flora of the Pondoland Centre of Endemism (PCE) recorded 2253 species in the combined checklist of four sites (Port St. Johns, Mkambati, Umtamvuna and Oribi Gorge). Of these 1 % species are endemic to Pondoland, representing 8.7% of the Species, 15% of the genera and 26% of the families of the combined flora. Forty-four percent ofthe combined flora was only recorded from one locality (between 17% and 26% of each flora) and only 12% of the flora was present in all four localities. Of the endemics only sixteen (8%) occurred in all four sites thus each site had its own complement of unique endemics and 21 % endemics were not recorded from any of the four sites. At species level the floras of Mkambati and Umtamvuna were the most similar, followed by that of Umtamvuna and Oribi Gorge. Port St Johns had the least in common with any of the othersites, but more in common with non-neighbours Umtamvuna and Oribi Gorge than with its nearest neighbour Mkambati. Mkambati and Umtamvuna had the largest proportion of PCE endemics and Port St Johns had the lowest. The four sites are quite similar at family level, sharing thirteen families in the top ten family list between them, but much less similar at generic level.
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- Date Issued: 2005
Towards the development of a protocol for rearing juvenile rock lobster, Jasus lalandii
- Authors: Esterhuizen, J. A
- Date: 2005
- Subjects: Lobsters -- Growth , Lobster fisheries -- South Africa , Crustacea -- Growth , Crustacea -- South Africa , Jasus lalandii , Lobster culture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5328 , http://hdl.handle.net/10962/d1005174 , Lobsters -- Growth , Lobster fisheries -- South Africa , Crustacea -- Growth , Crustacea -- South Africa , Jasus lalandii , Lobster culture
- Description: The diversification of current aquaculture practise in South Africa is crucial for the future development of the industry. The rock lobster, Jasus lalandii has been identified as a possible candidate species for shore based culture in systems similar to those used for abalone farming. Reduced Total Allowable Catch (TAC's) and an increased minimum legal size were implemented as management strategies to relieve fishing pressure on natural stocks of J. lalandii, and in turn led to increased market demand and a possible niche for farmed lobsters. High puerulus settlement along the Namibian coast, together with other favourable aquaculture attributes, indicate that the commercial grow out of wild harvested juveniles could be feasible. The aim of study was to investigate the feasibility of growing out rock lobsters in shore based systems in Namibia and South Africa. The experimental objectives of the project were to investigate the nutritional requirements, as well as the effect of stocking density and tank design on growth and survival of J. lalandii. The economic viability of shore based rock lobster farming was then evaluated based on the experimental results and typical capital requirements of an abalone farm in South Africa. Puerulus and early juvenile rock lobsters were collected in Luderitz, Namibia and transported to HIK Abalone Farm in Hermanus, South Africa where they were acclimated in black fibre glass tanks in a flow through system. The pueruli were stocked at 50, 75, 100 and 125/m² and early juveniles at 20, 30, 40 and 50/m² to test the effect of density on growth and survival. A comparative dietary study with both size classes was conducted using a mussel diet (Choromytilus meridionalis and Mytilus galloprovincialis), a commercial shrimp feed diet and a rotational diet comprising both mussels and shrimp feed. Tanks provided with "v-shaped" asbestos hides, PVC hides and no hides were compared to test the effect on growth rate and survival of pueruli. All treatments were conducted in triplicate. Temperatures were recorded twice daily while water quality parameters were tested every second week. The results indicate that density had a significant effect on growth and survival on post-pueruli. An initial stocking density of 75 post-pueruli/m² is regarded as optimal both in terms of the growth rate and biomass production per tank. No differences in growth and survival rates were attained in the early juvenile size class. This indicates that higher densities can be used to rear juvenile J. lalandii. No significant differences were obtained in growth rate when reared under different hide conditions although asbestos hides yielded significantly higher survival rates (93.3 %) compared to the tanks provided with PVC hides (74.04 %) and no hides (77.8 %, p≤0.05). As a result of the high survival rate observed in tanks provided with "V-shaped" asbestos hides, these tanks also yielded higher biomass production per tank (297.8 grams) compared to the tanks provided with PVC hides and no hides (261.09 and 260.5 grams respectively). In the diet trials, growth rates of post pueruli and juvenile lobsters fed the mussel and rotational (mussel and shrimp feed) diets did not differ significantly, however, growth rates of lobsters fed the shrimp feed only diet was significantly lower than both the mussel and rotational diets (p≤0.05). Lobsters fed the mussel diet yielded significantly higher survival rates compared to lobsters fed either the rotational diet or shrimp feed diet only. An imbalance in the fatty acid profile of the shrimp feed could have been a major contributing factor to the poor growth and survival in lobsters fed the shrimp feed only diet as the level of linoleic acid (LOA) was exceptionally high resulting in a high (n -6):(n-3) ratio (1.01) compared to the mussel and rotational diets (0.17 and 0.56 respectively). The low level of arachidonic acid (ARA) in the shrimp feed diet could be a further contributing factor explaning for the poor growth and survival of juvenile rock lobsters. An economic feasibility study was conducted at the end of the experimental phase by modelling a hypothetical shore based rock lobster farm. A projection of production costs and revenues was based on the typical costs of a shore based abalone farm and the current market prices for wild harvested J. lalandii. The economic viability was evaluated using benefit-cost ratios, payback period, internal rate of return and breakeven analysis. Sensitivity analyses revealed that the projected lobster growth and survival rates were the main biological factors influencing the economic feasibility of the hypothetical rock lobster farm. An assumed four year grow out period at a low stocking density yielded more lucrative internal rate of return (IRR), benefit-cost ratio, payback period and net present values (NPV) than a five year grow out period at a high density. The four year grow out scenario proved to be more robust to the fluctuating Rand/US$ exchange rate and could accommodate a lower lobster survival rate. Results presented in this study indicate that rock lobster farming is a marginal commercial prospect based on current production performance and costs. Further research to develop effective puerulus collection techniques as well as to make lobster grow out technology more cost effective is required.
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- Date Issued: 2005
The role of anthropogenic disturbance in the creation of a socio-ecological landscape
- Authors: Fox, Helen Elizabeth
- Date: 2005
- Subjects: Human ecology -- South Africa -- Kat River Valley , Land use -- Environmental aspects-- South Africa -- Kat River Valley , Landscapes -- Social aspects -- South Africa -- Kat River Valley , Landscape ecology -- South Africa -- Kat River Valley , Vegetation and climate -- South Africa -- Kat River Valley , Xhosa (African people) -- Folklore , Kat River Valley (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4772 , http://hdl.handle.net/10962/d1008061 , Human ecology -- South Africa -- Kat River Valley , Land use -- Environmental aspects-- South Africa -- Kat River Valley , Landscapes -- Social aspects -- South Africa -- Kat River Valley , Landscape ecology -- South Africa -- Kat River Valley , Vegetation and climate -- South Africa -- Kat River Valley , Xhosa (African people) -- Folklore , Kat River Valley (South Africa)
- Description: The aim of this thesis is to examine the role of anthropogenic disturbance in the creation of a socio-ecological landscape. Three key questions were answered: what impact has past anthropogenic disturbance had on present vegetative characteristics; what value did this disturbed landscape have to local people; how did the local peoples' worldviews and eco-cosmologies influence how they perceived, valued and managed their landscape? Research was based in a rural, predominantly amaXhosa village in the Kat River valley, Eastern Cape, South Africa. Four major landscape components characterised the environment, namely dense forests, former grazing lands, abandoned fields and old settlements. A combination of qualitative and quantitative methods were adopted, consisting of a vegetation and soil survey and social science methods including semistructured interviews, focus groups, transect walks and participant observation. The key finding was that anthropogenic disturbance is necessary to enhance the potential of the area to support human habitation. However, anthropogenic disturbance can have positive or negative effects for both local people and the environment. An intermediate level of disturbance is a key factor leading to a resilient socio-ecological system. Various anthropogenic disturbances have had significant affects on vegetation characteristics in terms of species richness, and a change in vegetation composition and species heights. Of the three anthropogenic landscapes examined, former grazing lands were the least intensively disturbed. They were also more species rich and structurally diverse than areas that were under agricultural production or used as a settlement. Dense forests, although the least desirable landscape to local people were, together with sacred pools, sources of ecosystem renewal and played a critical role during times of disturbance. Anthropogenic disturbance has added value to local people's livelihoods; this disturbance has resulted in a patchy landscape that supplies a wide variety of resources to local people. Local people are dependent on their environment for their basic needs; this has encouraged sustainable management practices. The local AmaXhosa still retain many elements of their traditional worldview, which has many characteristics of Animism. The environment is an integral component of their belief system and certain sites and species have significant cultural value and are protected.
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- Date Issued: 2005
Coastal dune dynamics and management at the Bushman's River mouth, Kenton-on-Sea
- Authors: Fraser, Claire
- Date: 2005
- Subjects: Sand dunes -- South Africa -- Management , Sand dune ecology -- South Africa -- Kenton-on-Sea , Coastal ecology -- South Africa -- Kenton-on-Sea , Coastal zone management -- South Africa -- Kenton-on-Sea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4192 , http://hdl.handle.net/10962/d1003761 , Sand dunes -- South Africa -- Management , Sand dune ecology -- South Africa -- Kenton-on-Sea , Coastal ecology -- South Africa -- Kenton-on-Sea , Coastal zone management -- South Africa -- Kenton-on-Sea
- Description: A study of the dynamics and functions of a coastal dunefield at the Bushman’s River Mouth in Kenton-on-Sea was conducted to provide essential information for the formulation of a management strategy based on an ecosystem approach. Bi-monthly tacheometric surveys were undertaken at the Westbourne Road Car Park dunefield to investigate the sedimentological processes. Changes in the topography, the amount and rate of sand accretion were monitored. Accretion and progradation were the dominant long-term sedimentological processes at the site. Long-term spatial variation in these processes corresponded to the temporal pattern of sedimentation in the river mouth while short-term variation resulted from dynamics inherent to the types of dunes present. Factors affecting aeolian accretion at the site are topography, river mouth configuration, dune types and the presence of vegetation as well as the seasonal wind regime. A vegetation study, recording species present, cover and abundance of the plants along the surveyed transects, was also conducted to examine the progressive establishment of vegetation. Five plant communities were discerned namely, saltmarsh, pioneer, enriched pioneer, open scrub, closed scrub and scrub-thicket communities. The spatial distribution of these communities coincided with the north-westerly development of the sandbank. A successional trend in community change occurred along a gradient of increasing diversity, complexity and age. Initially, autogenic changes wrought by pioneer species facilitated the recruitment and survival of other species and commenced succession at the study site. Multiple successional pathways were discussed in relation to subsequent species establishment that occurred after facilitation. The amalgamation of ecological data with a social study in the form of public perception surveys allowed for a greater understanding of the present interactions between the ecosystem and the demands placed on it. The area primarily functions as a recreational site that offered numerous beach and water-related activities. A lack of information regarding the ecology and management programmes influenced public perception of the environment and management issues in general. Different management perspectives and the use of the information presented in this study were also discussed.
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- Date Issued: 2005
Establishment and impact of the sap-sucking mirid, Falconia intermedia (Distant) (Hemiptera: Miridae) on Lantana camara (Verbenaceae) varieties in the Eastern Cape Province, South Africa
- Authors: Heshula, Unathi-Nkosi Lelethu Peter
- Date: 2005
- Subjects: Lantana camara -- South Africa -- Eastern Cape , Biological pest control agents -- South Africa -- Eastern Cape , Hemiptera -- South Africa -- Eastern Cape , Miridae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5832 , http://hdl.handle.net/10962/d1008085
- Description: The biological control of the weedy complex Lantana camara (L.) (Verbenaceae) has been ongoing in South Africa for over 40 years. Despite this, the weed is still not under sufficient control and continues to invade new territories in the country. The biological control programme needs to be bolstered with releases of new and potentially damaging biological control agents. A promising biological control agent endemic to Central America, Falconia intermedia (Distant) (Hemiptera: Miridae), was imported into quarantine from Jamaica in 1994. This agent was released on sites in KwaZulu-Natal and Limpopo provinces of South Africa in 1999. Even though it initially established and damaged L. camara, populations died out at most of the release sites. As varietal difference and adverse climate have been cited as the reason for non-establishment and ineffective control in L. camara biocontrol programmes worldwide, this study attempts to investigate the role that these two factors play in this weed herbivore relationship. Laboratory no-choice trials were conducted to determine the varietal performance of F intermedia, among five Eastern Cape varieties of the weed from East London, Whitney Farm, Heather Glen Farm, Port Alfred and Lyndhurst Farm, and a variety from the Plant Protection Research Institute (PPRI), Pretoria. However, there were differences in performance as the adult mirids performed better on white-pink varieties from Whitney Farm and Heather Glen Farm. To test varietal preference in field conditions, field releases of F intermedia were also made at East London, Whitney Farm, Heather Glen Farm, Port Alfred and Lyndhurst Farm. Post release evaluations were conducted monthly for two years (2002 and 2003). The insect established at East London and Whitney Farm, both of which have white-pink varieties. Insect populations quickly died out at the Lyndhurst Farm and Port Alfred sites, which have dark pink varieties. It is suggested that field conditions may have resulted in poor plant quality and led indirectly to varietal preference, and to non-establishment at these two sites. With the onset of cooler weather, populations disappeared at Heather Glen Farm. This suggested that F. intermedia was suitable for release in more subtropical areas within South Africa where climatic conditions would be suitable throughout the year. The mirid performed well at Whitney Farm, resulting in significant reduction in plant growth parameters such as height and percentage cover, and increasing the cover of other flora growing beneath L. camara plants. Finally, ways to improve the efficacy of this agent are considered in an effort towards better control of L. camara in South Africa.
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- Date Issued: 2005
Phylogeography and comparative ecophysiology of Chrysanthemoides Turn. Ex Medik. (Tribe Calenduleae)
- Authors: Howis, Seranne
- Date: 2005
- Subjects: Chrysanthemoides , Phylogeny , Ecophysiology , DNA , Plant genetics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4256 , http://hdl.handle.net/10962/d1008189
- Description: Chrysanthem Oides is a common Southern African shrub that grows in a variety of habitats. From coastal shrubland and fynbos to mountainous areas as far north as Kenya. The genus has two species and 8 subspecies. The diagnoses and delimitation of which have been based almost exclusively on morphological characteristics. This project aims to investigate, with the use of phylogenetic species concepts. The validity of these subspecies. Unlike biological species concepts that rely on reproductive isolation as a defining character of a species. Phylogenetic species concepts (PSC) are concerned with delimiting evolutionary significant units (ESUs). ESUs are evolutionarily isolated lineages, and under the PSC a species is an aggregation of organisms consistently diagnosable by a fixed character or combination of characters. This project therefore searched for genetic and physiological characters by which to delimit ESUs within the Cill), samhemoides genus. DNA sequencing was used to investigate the genetic characters, while gas exchange studies were used to investigate the ecophysiological characters. DNA sequence analysis indicated that the ESUs can be diagnosed by genetic means and that one species may be of hybrid origin. Field studies of three disparate genetically identifiable ESUs from three disparate climates found that there are noticeable differences in ecophysiological responses of these ESUs in the field. Plants from each ESU were transferred to a greenhouse and grown under identical conditions for several months and compared to determine if these traits are inherent, or elastic in relation to environmental conditions. Under simulated high rainfall conditions. There does not appear to be a significant difference in the photosynthetic traits.
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- Date Issued: 2005
Service provisioning in two open-source SIP implementation, cinema and vocal
- Authors: Hsieh, Ming Chih
- Date: 2013-06-18
- Subjects: Real-time data processing , Computer network protocols , Internet telephony , Digital telephone systems , Communication -- Technological innovations
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4687 , http://hdl.handle.net/10962/d1008195 , Real-time data processing , Computer network protocols , Internet telephony , Digital telephone systems , Communication -- Technological innovations
- Description: The distribution of real-time multimedia streams is seen nowadays as the next step forward for the Internet. One of the most obvious uses of such streams is to support telephony over the Internet, replacing and improving traditional telephony. This thesis investigates the development and deployment of services in two Internet telephony environments, namely CINEMA (Columbia InterNet Extensible Multimedia Architecture) and VOCAL (Vovida Open Communication Application Library), both based on the Session Initiation Protocol (SIP) and open-sourced. A classification of services is proposed, which divides services into two large groups: basic and advanced services. Basic services are services such as making point-to-point calls, registering with the server and making calls via the server. Any other service is considered an advanced service. Advanced services are defined by four categories: Call Related, Interactive, Internetworking and Hybrid. New services were implemented for the Call Related, Interactive and Internetworking categories. First, features involving call blocking, call screening and missed calls were implemented in the two environments in order to investigate Call-related services. Next, a notification feature was implemented in both environments in order to investigate Interactive services. Finally, a translator between MGCP and SIP was developed to investigate an Internetworking service in the VOCAL environment. The practical implementation of the new features just described was used to answer questions about the location of the services, as well as the level of required expertise and the ease or difficulty experienced in creating services in each of the two environments. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Investigating call control using MGCP in conjuction with SIP and H.323
- Authors: Jacobs, Ashley
- Date: 2005 , 2005-03-14
- Subjects: Communication -- Technological innovations , Digital telephone systems , Computer networks , Computer network protocols , Internet telephony
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4631 , http://hdl.handle.net/10962/d1006516 , Communication -- Technological innovations , Digital telephone systems , Computer networks , Computer network protocols , Internet telephony
- Description: Telephony used to mean using a telephone to call another telephone on the Public Switched Telephone Network (PSTN), and data networks were used purely to allow computers to communicate. However, with the advent of the Internet, telephony services have been extended to run on data networks. Telephone calls within the IP network are known as Voice over IP. These calls are carried by a number of protocols, with the most popular ones currently being Session Initiation Protocol (SIP) and H.323. Calls can be made from the IP network to the PSTN and vice versa through the use of a gateway. The gateway translates the packets from the IP network to circuits on the PSTN and vice versa to facilitate calls between the two networks. Gateways have evolved and are now split into two entities using the master/slave architecture. The master is an intelligent Media Gateway Controller (MGC) that handles the call control and signalling. The slave is a "dumb" Media Gateway (MG) that handles the translation of the media. The current gateway control protocols in use are Megaco/H.248, MGCP and Skinny. These protocols have proved themselves on the edge of the network. Furthermore, since they communicate with the call signalling VoIP protocols as well as the PSTN, they have to be the lingua franca between the two networks. Within the VoIP network, the numbers of call signalling protocols make it difficult to communicate with each other and to create services. This research investigates the use of Gateway Control Protocols as the lowest common denominator between the call signalling protocols SIP and H.323. More specifically, it uses MGCP to investigate service creation. It also considers the use of MGCP as a protocol translator between SIP and H.323. A service was created using MGCP to allow H.323 endpoints to send Short Message Service (SMS) messages. This service was then extended with minimal effort to SIP endpoints. This service investigated MGCP’s ability to handle call control from the H.323 and SIP endpoints. An MGC was then successfully used to perform as a protocol translator between SIP and H.323.
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- Date Issued: 2005
An investigation into improved ionospheric F1 layer predictions over Grahamstown, South Africa
- Authors: Jacobs, Linda
- Date: 2005
- Subjects: Ionosphere , Ionospheric electron density -- South Africa -- Grahamstown , Neural networks (Computer Science)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5511 , http://hdl.handle.net/10962/d1008094 , Ionosphere , Ionospheric electron density -- South Africa -- Grahamstown , Neural networks (Computer Science)
- Description: This thesis describes an analysis of the F1 layer data obtained from the Grahamstown (33.32°S, 26.500 E), South Africa ionospheric station and the use of this data in improving a Neural Network (NN) based model of the F1 layer of the ionosphere. An application for real-time ray tracing through the South African ionosphere was identified, and for this application real-time evaluation of the electron density profile is essential. Raw real-time virtual height data are provided by a Lowell Digisonde (DPS), which employs the automatic scaling software, ARTIST whose output includes the virtual-toreal height data conversion. Experience has shown that there are times when the ray tracing performance is degraded because of difficulties surrounding the real-time characterization of the F1 region by ARTIST. Therefore available DPS data from the archives of the Grahamstown station were re-scaled manually in order to establish the extent of the problem and the times and conditions under which most inaccuracies occur. The re-scaled data were used to update the F1 contribution of an existing NN based ionospheric model, the LAM model, which predicts the values of the parameters required to produce an electron density profile. This thesis describes the development of three separate NNs required to predict the ionospheric characteristics and coefficients that are required to describe the F1 layer profile. Inputs to the NNs include day number, hour and measures of solar and magnetic activity. Outputs include the value of the critical frequency of the F1 layer, foF1, the real height of reflection at the peak, hmFl, as well as information on the state of the F1 layer. All data from the Grahamstown station from 1973 to 2003 was used to train these NNs. Tests show that the predictive ability of the LAM model has been improved by incorporating the re-scaled data.
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- Date Issued: 2005
The effect of personalised adjustments to computer workstations on the efficiency and physical comfort of computer operators
- Authors: James, Genevieve
- Date: 2005
- Subjects: Human engineering , Engineering design , Work environment , Microcomputer workstations -- Health aspects , Occupational diseases -- Prevention
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5112 , http://hdl.handle.net/10962/d1005190 , Human engineering , Engineering design , Work environment , Microcomputer workstations -- Health aspects , Occupational diseases -- Prevention
- Description: The present study sought to investigate the effects of a Standard workstation, designed for “average” users, on an anthropometrically diverse sample of computer operators, and to assess whether physical and perceptual responses, as well as performance efficiency were dependent on stature. Further investigation assessed the influence of personalised adjustments to the Standard workstation, based on the anthropometric characteristics of the subjects, as well as the introduction of a custom-designed ‘floating’ wrist support, on subject responses. All subjects (n=30) were tested in each of the three workstations: Standard, Personalised and Wrist Support. For analysis of responses in the Standard workstation, subjects were divided into three groups depending on their stature: Short (<1650mm), Medium (1650mm to 1800mm), Tall (>1800mm). The musculoskeletal responses indicated that Tall subjects were forced to adopt the most awkward general body postures as a result of the low computer screen. However, the low screen allowed for the Short subjects to adopt the most natural general body postures, although levels of muscular activity in the upper trapezius suggest that the muscular load imposed on both Short and Tall subjects was significantly greater than that imposed on the Medium subjects. In addition, the Medium subjects’ perceptions of the Standard workstation dimensions support the fact that this workstation was better suited to users with “average” morphologies. The responses elicited in the Personalised and Wrist Support workstations were improved significantly when compared to the Standard workstation. Joint angles were more natural, upper trapezius EMG was reduced, standard of performance improved and perceptual responses indicated a diminished incidence of body and visual discomfort, as well as greater perceived satisfaction with these workstation dimensions. The improved physical responses suggest a decrease in the risk of developing cumulative trauma disorders. Although subjects were unaccustomed to the wrist support device, this workstation demonstrated a further reduction in the range of wrist angles, as well as a general positive attitude towards the concept.
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- Date Issued: 2005
Puerulus and early juvenile recruitment of the rock lobster jasus lalandii in relation to the environment at Lüderitz Bay, Namibia
- Authors: Keulder, Felicia Juanita
- Date: 2005
- Subjects: Jasus lalandii , Lobster fisheries -- Namibia , Lobster culture -- Namibia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5315 , http://hdl.handle.net/10962/d1005160 , Jasus lalandii , Lobster fisheries -- Namibia , Lobster culture -- Namibia
- Description: A global decline in the lobster fishery has necessitated the growout of wild-caught postlarvae (pueruli) on farms to meet the worlds lobster demand. A limited knowledge on puerulus settlement in the West Coast rock lobster (Jasus lalandii) constitutes a major barrier for J. lalandii culture development in Southern Africa. As an extension of an existing long-term puerulus monitoring program of the Ministry of Fisheries and Marine Resources of Namibia, the present study monitored puerulus recruitment (supply), puerulus condition and juvenile growth of J. lalandii and their relationship with environmental variables on an oyster farm at Lüderitz Bay, Namibia. Implications for lobster aquaculture and fisheries management are discussed. Oyster bags, crates and collectors were sampled daily, weekly and bimonthly, for pueruli and juveniles, respectively, between August 2001 and July 2003 to establish spatialtemporal patterns in their distribution. Animals were transported to the laboratory for morphometric analyses where they were staged as puerulus (Pt = transparent; Pp = pigmented) and juvenile lobsters. The carapace lengths (0.1mm CL) and wet weights (0.01gram) of animals were recorded. Carapace length frequencies were subjected to Modal Progression analysis to identify early juvenile cohorts and calculate their growths. The weight-length ratios calculated and percentage fat content were used as indices of nutritional condition. Seasonal peaks in puerulus and juvenile abundance were estimated using Periodic Regression analysis. The relationships of puerulus and juvenile abundance with respect to sea surface temperature (SST), wind speed-direction, moon phase, swell and tidal range were established using time lag analysis (ADL). Transparent pueruli were randomly distributed on oyster bags and crates, and also between collector sites, suggesting that they have no particular settlement preference for certain areas on these structures (p>0.05; n=150). Pigmented puerulus (p<0.05; n=566) and juveniles (p<0.05; n=3528) preferred structurally complex structures that provided better shelter such as crates, which were more sheltered from sea swells, compared to oyster bags. Among oyster rafts, pigmented puerulus and juveniles preferred occupying the sheltered and centred raft areas, which provided better protection from wave action, compared to exposed raft areas, which were exposed to the incoming swell. Puerulus and juvenile lobster appeared to recolonise adjacent bags and crates, and possibly migrate to the substrate and off the farm due to the disturbance of the habitat during harvesting. The booth crevice collector was more efficient in collecting puerulus possibly due to the high structurally complex habitat and the better shelter they provided compared to the hogshair collector. The occupation of structurally complex habitats by early benthic stages may be related to morphological changes associated with metamorphosis. Puerulus settlement peaked during early spring (August-September) and was related to low sea surface temperatures (12.3°C), strong south winds, and moderate swells in the week preceding settlement. These conditions are synonymous with the “spring upwelling” season of the Lüderitz upwelling cell in the central Benguela current region. Puerulus nutritional condition peaked during this period possibly due to high levels of planktonic food (due to high nutrients from upwelling) that was available to the late stage phyllosoma larvae further offshore. A “good” puerulus settlement year during 2000 was associated with lower than average SST (11.6°C) and strong south winds and was followed by “poor” puerulus settlement years during 2001 and 2002 when higher than average SST were experienced with below average wind speeds experienced during 2001 only. Puerulus of J. lalandii recruited in high numbers into Lüderitz Bay during the new moon, full moon and last quarter moon phases by making use of the increased tides during these phases of the lunar cycle. Peak juvenile abundance during early summer (November-December) lagged by 8 weeks was significantly correlated with peak puerulus abundance. Puerulus size was similar (8.5–9.7mm CL) over the study period. Juvenile cohorts, 9.9-20.6mm CL in size recruited in 2 distinct waves in August 2001-June 2002 and July 2002-February 2003. The 2001/2002 recruits had higher monthly growth increments and better nutritional condition compared to 2002/2003 recruits. Peak frequencies of 2nd moult juveniles (9.9- 10.8mm CL) occurred in September of both recruitment events with nutritional condition being poorest during the 2002/2003 event. The nutritional condition of the settling puerulus did not appear to be related to the subsequent growth of juvenile lobster. These preliminary results suggest that pueruli recruit once a year mainly during late winter/ early spring under conditions typical of coastal upwelling in the Benguela current near Lüderitz. It appears that puerulus settlement is driven by favourable shoreward currents, which help in transporting puerulus to their settlement grounds. Puerulus supply is highly variable between years, which appears to be consistent with a highly variable Benguela environment. The oyster farm supported a substantial standing stock averaging 123 800 lobster per day (range: 22 600-325 300 lobster per day) of which 26 600 were puerulus and 97 200 were juveniles, that may have been harvested once off on any given day during the sample period. Monthly harvesting of the total lobster standing stock during the one and a half year sampling period was just over 2 million lobsters (505 700 pueruli and 1.85 million juveniles) and would potentially have been sufficient seed for a 200 ton lobster production in puerulus lobster growout facilities. In the light of high inter-annual variation in puerulus settlement observed at Lüderitz, the seed supply at the Lüderitz oyster farm is clearly limited and therefore it is prudent that other sites of high puerulus settlement is established before large commercial scale lobster growout can commence. The migration of lobster off the farm suggested that these lobsters potentially recruit to the fishery and therefore harvesting the entire standing stock on the farm may have adverse negative effects on the wild fishery. A policy decision will be required as to whether more efficient harvesting of lobster from the farm should be allowed. If puerulus removal in J. lalandii for aquaculture is allowed in the near future, it should be conducted on an experimental scale or small commercial scale until the potential effects on recruitment into the fishery are clearer.
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- Date Issued: 2005
Formulation and assessment of verapamil sustained release tablets
- Authors: Khamanga, Sandile Maswazi Malungelo
- Date: 2005
- Subjects: Verapamil , Tablets (Medicine) , Drugs -- Administration , Cardiovascular agents , Calcium -- Antagonists , Drugs -- Controlled release
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3869 , http://hdl.handle.net/10962/d1018236
- Description: The oral route of drug administration is most extensively used due to the obvious ease of administration. Verapamil hydrochloride is a WHO listed phenylalkylarnine, L-type calcium channel antagonist that is mainly indicated for cardiovascular disorders such as angina pectoris, supraventricular tachycardia and hypertension. Due to its relatively short half-life of approximately 4.0 hours, the formulation of a sustained-release dosage form is useful to improve patient compliance and to achieve predictable and optimized therapeutic plasma concentrations. Direct compression and wet granulation were initially used as methods for tablet manufacture. The direct compression method of manufacture produced tablets that exhibited formulation and manufacturing difficulties. Mini-tablets containing veraparnil hydrochloride were then prepared by wet granulation using Surelease® E-7-19010.and Eudragit® NE 30D as the granulating agents after which the granules were incorporated with an hydrophilic matrix material, Carbopol® 974P NF. Granule and powder blends were evaluated using the angle of repose, loose and tapped bulk density, Can's compressibility index, Hausner's ratio and drug content. Granules with good flow properties and satisfactory compressibility were used for further studies. Tablets were subjected to thickness, diameter and weight variation tests, crushing strength, tensile strength, friability and content uniformity studies. Tablets that showed acceptable pharmaco-technical properties were selected for further analysis. Drug content uniformity and dissolution release rates were determined using a validated isocratic HPLC method. Initially, USP apparatus 1 and 3 dissolution apparatus were used to determine in-vitro drug release rates from the formulations over a 22-hour period. USP apparatus 3 was finally selected as it offers the advantages of mimicking, in part, the changes in the physicochemical environment experienced by products in the gastro-intestinal tract. Differences in release rates between the test formulations and a commercially available product, Isoptin® SR were observed at different pH's using USP apparatus 1. The release of veraparnil hydrochloride from matrix tablets was pH dependent and was markedly reduced at higher pH values. This may be due, in part, to the poor solubility of veraparnil hydrochloride at these pH values and also the possible interaction of verapamil hydrochloride with anionic polymers used in these formulations. Swelling and erosion behaviour of the tablets were evaluated and differences in behaviour were observed which may be attributed to the physico-chemical characteristics of the polymers used in this study. In-vitro dissolution profiles were characterized by the difference (j1) and similarity factor (j2) and also by a new similarity factor, Sct. In addition, the mechanism of drug release from these dosage forms was mainly evaluated using the Korsmeyer-Peppas model and the kinetics of drug release assessed using other models, including Zero order, First order, Higuchi, HixsonCrowell, Weibull and the Baker-Lonsdale model. Dissolution kinetics were best described by application of the Weibull model, and the Korsmeyer-Peppas model. The release exponent, n, confirmed that drug release from these dosage forms was due to the mixed effects of diffusion and swelling and therefore, anomalous release kinetics are predominant. In conclusion, two test batches were found to be comparable to the reference product Isoptin® SR with respect to their in-vitro release profiles.
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- Date Issued: 2005
The development of the emerging technologies sustainability assessment (ETSA) and its application in the design of a bioprocess for the treatment of wine distillery effluent
- Authors: Khan, Nuraan
- Date: 2005
- Subjects: Technology assessment , Wine and wine making -- Waste disposal , Distilleries -- Waste disposal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3953 , http://hdl.handle.net/10962/d1004012 , Technology assessment , Wine and wine making -- Waste disposal , Distilleries -- Waste disposal
- Description: Emerging Technologies Sustainability Assessment (ETSA) is a new technology assessment tool that was developed in order to compare emerging processes or technologies to existing alternatives. It utilizes infoIDlation modules, with the minimum use of resources such as time and money, in order to deteIDline if the process under development is comparatively favourable and should be developed beyond the early conceptual phase. The preliminary ETSA is vital in order to identify the gaps in the existing information and the specific methodologies to be used for data capture and analysis. The use of experimental design tools, such as Design-Expert, can facilitate rapid and efficient collection of necessary data and fits in well with the rationale for the ETSA. Wine distillery effluent (vinasse) is the residue left after alcohol has been distilled from fennented grape juice. It is an acidic, darkly coloured effluent, with a high COD and polyphenol content. The most popular method of disposal of this effluent, land application, is no longer viable due to stricter legislation and pressure on the industry to better manage its wastes. Although the ability of whiterot fungi to degrade a number of pollutants is well-known, fungal treatment of wine distillery effluent is still in the conceptual phase. The perfoIDlance of the fungal remediation system was assessed experimentally in terms of COD removal and laccase production using Design-Expert. Although Pycnoporus sanguine us was found to be most efficient at COD removal (85%) from 30% vinasse, laccase production was low (0.021 U/I). The optimum design for economically viable fungal treatment used Trametespubescens. This fungus was able to remove over 50% of the COD from undiluted vinasse while producing almost 800U/l of the valuable laccase enzyme within three days. Since the effluent from the fungal system did not meet the legal limits for wastewater disposal, a two-stage aerobicanaerobic system is suggested to improve the quality of the effluent prior to disposal. The ETSA was used to assess the fungal technology in relation to the two current methods of vinasse treatment and disposal, namely land application and anaerobic digestion. Based on the ETSA, which considered environmental, social and economic impacts, the fungal system proved to be potentially competitive and further development of the technology is suggested.
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- Date Issued: 2005
The mining and visualisation of application services data
- Authors: Knoetze, Ronald Morgan
- Date: 2005
- Subjects: Data mining -- South Africa , Computer algorithms , Network performance (Telecommunication) -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10480 , http://hdl.handle.net/10948/451 , Data mining -- South Africa , Computer algorithms , Network performance (Telecommunication) -- Research -- South Africa
- Description: Many network monitoring tools do not provide sufficiently in-depth and useful reports on network usage, particularly in the domain of application services data. The optimisation of network performance is only possible if the networks are monitored effectively. Techniques that identify patterns of network usage can assist in the successful monitoring of network performance. The main goal of this research was to propose a model to mine and visualise application services data in order to support effective network management. To demonstrate the effectiveness of the model, a prototype, called NetPatterns, was developed using data for the Integrated Tertiary Software (ITS) application service collected by a network monitoring tool on the NMMU South Campus network. Three data mining algorithms for application services data were identified for the proposed model. The data mining algorithms used are classification (decision tree), clustering (K-Means) and association (correlation). Classifying application services data serves to categorise combinations of network attributes to highlight areas of poor network performance. The clustering of network attributes serves to indicate sparse and dense regions within the application services data. Association indicates the existence of any interesting relationships between different network attributes. Three visualisation techniques were selected to visualise the results of the data mining algorithms. The visualisation techniques selected were the organisation chart, bubble chart and scatterplots. Colour and a variety of other visual cues are used to complement the selected visualisation techniques. The effectiveness and usefulness of NetPatterns was determined by means of user testing. The results of the evaluation clearly show that the participants were highly satisfied with the visualisation of network usage presented by NetPatterns. All participants successfully completed the prescribed tasks and indicated that NetPatterns is a useful tool for the analysis of network usage patterns.
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- Date Issued: 2005