Vitamin E supplementation and secondary metabolites interactions and effects on melanoma growth
- Authors: Ottino, Paulo
- Date: 1997
- Subjects: Vitamin E Melanoma
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4016 , http://hdl.handle.net/10962/d1004076
- Description: The present study was undertaken to determine the effects and possible mechanism of action of vitamin E succinate on malignant murine melanoma (BL6) and non-malignant monkey kidney (LLCMK) cell growth in vitro. Studies revealed that supplementation of 5, 7 and lOJLg/ml vitamin E succinate significantly inhibited BL6 cell growth, while in LLCMK cells no significant increase or decrease in growth was observed. The actual mechanism by which vitamin E succinate inhibits BL6 cell growth is at present unclear. Studies have suggested a radical or oxidant involvement in a number of degenerative diseases such as cancer, and that supplementation of antioxidant vitamins such as vitamin E may function to reduce cancer cell growth by quenching free radical species and preventing lipid peroxidation. In addition to its antioxidant role in a cell, vitamin E is believed to modulate the activities of various enzymes and metabolites in the eicosanoid pathway. Hence, this study investigated the effects of vitamin E succinate supplementation on free radical and lipid peroxidation levels, as well as the activities of various enzymes and metabolites ill the eicosanoid pathway. Throughout this study, emphasis was placed on BL6 melanoma cells since the magnitude of the relationship between LLCMK growth and the levels of various enzymes and metabolites in the eicosanoid pathway varied considerably from one experiment to another and did not show the consistent trend found with the BL6 cells. A decrease in cell growth was found to be accompanied by a concomitant increase rather than a decrease in the levels of free radicals and lipid peroxidation, suggesting that the growth inhibitory effects of vitamin E succinate on BL6 cells in vitro was not due to its antioxidant properties associated with the vitamin E component, but rather due to one or more of its other potential roles within the cell. This proposal was further strengthened by findings that vitamin E succinate, a non-physiological antioxidant in its esterified form, did not undergo significant cleavage to free vitamin E in the BL6 cells. Vitamin E succinate is believed to modulate membrane bound enzyme activities through physicochemical interactions with membrane lipids and changes in membrane fluidity. Hence, this study investigated the role of vitamin E succinate in modulating the activity of various enzymes and secondary messengers in the eicosanoid pathway. Supplementation of l-lOjLg/ml vitamin E succinate resulted in an overall increase in phospholipase A2 activity while cyclooxygenase and adenyl ate cyclase activities were found to be significantly increased at vitamin E succinate concentrations of 7 and WjLg/ml respectively. A significant increase in" 5-LOX activity was observed a! 10jLg/mi supplementation. The suggestion that vitamin E succinate modulates membrane bound enzyme activities was further strengthened by uptake and cellular distribution studies, which showed significantly higher levels of vitamin E succinate in membrane fractions of BL6 cells when compared with stroma fractions. Another factor which could account for elevated PLA2,-5-LOX and COX activities in BL6 cells as a result of vitamin E succinate supplementation, was that of intracellular calcium levels. Supplementation of BL6 cells with 1-7 jLg/ml vitamin E succinate resulted in an overall increase in intracellular calcium levels. These changes in calcium levels however were positively correlated with changes in PLA2 activity only. Since the rate of prostaglandin synthesis is controlled by phospholipase A2 activity, and net prostagiandin production is dependant on cyclooxygenase activity, the effects of vitamin E succinate supplementation on prostaglandin levels in BL6 cells was determined. Vitamin E succinate supplementation resulted in a significant decrease in prostaglandin D2 levels at vitamin E succinate concentrations of 3, 5, 7 and lOjLg/ml respectively, while prostaglandin F2a levels were significantly decreased at 1-10jLg/ml vitamin E succinate. The increases in prostaglandin E2 and 12 levels were inversely related to BL6 cell growth suggesting that both prostaglandins may act as negative regulators of BL6 cell growth. When comparing prostaglandin E2 levels to prostaglandin 12 levels in BL6 cells, significantly higher levels of prostaglandin E2 were found, suggesting that vitamin E succinate effects were mediated primarily through an increase in prostaglandin E2 levels. Furthermore, prostaglandin E2 levels are believed to modulate adenylate cyclase activity. It is therefore reasonable to conclude that the increased adenyl ate cyclase activity found in BL6 cells was dependant on prostaglandin E2 levels, since increases in prostaglandin E2 levels at 7 and lOjLg/ml vitamin E succinate correlated with an increase in adenylate cyclase activity and cyclic adenosine monophosphate levels. Thus it appeared that the observed inhibitory effects of vitamin E succinate supplementation on BL6 cell growth was not due to the antioxidant properties associated with the vitamin E component of the vitamin E succinate molecule, but was rather mediated in part through a cascade effect initiated by phospholipase A2 activation and archidonic acid release. This initial effect then appeared to result in an increase in cyclooxygenase activity and activation of a prostaglandin E2-adenylate cyclase-cyclic adenosine monophosphate linked system, ultimately altering cyclic adenosine monophosphate levels and inhibiting BL6 cell growth. This was confirmed when BL6 cells were supplemented with indomethacin, a cyclooxygenase inhibitor. Supplementation with the inhibitor resulted in vitamin E succinate having no inhibitory effects on BL6 cell growth. Furthermore, when comparing the levels of prostaglandin ~, adenylate cyclase activity and cyclIC adenosine monophosphate in the indomethacin treated cultures to non-indomethacin treated cultures, markedly lower levels of these metabolites were found in the indomethacin treated cultures. The cause of the increase in free radical and lipid peroxidation levels in BL6 cells following vitamin E succinate supplementation was further investigated. Cyclooxygenase enzymes are believed to generate free radical species and contribute to lipid peroxidation levels during catalytic activity. Markedly lower levels of free radicals and lipid peroxidation in indomethacin treated cultures were found when compared with vitamin E succinate treated cultures alone, suggesting that the increases in free radical and lipid peroxidation levels in BL6 cells supplemented with vitamin E succinate were indirectly due to an increase in cyclooxygenase activity in these cells.
- Full Text:
- Date Issued: 1997
Urban agriculture: advocacy and practice: a discursive study with particular reference to three Eastern Cape centres
- Authors: Webb, Nigel Leigh
- Date: 1997
- Subjects: Urban agriculture -- South Africa -- Eastern Cape Land use, Urban -- South Africa -- Eastern Cape Agriculture -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2095 , http://hdl.handle.net/10962/d1002658
- Description: The purpose of this thesis is to explain the continued advocacy of 'urban agriculture' (UA) in the literature in the face of the seemingly modest role played by cultivation in practice. The analytical framework adopted isrthat of discourse, the theoretical underpinnings of which are derived from the early works of Foucault, and applications such as those of Escobar (1989; 1991) and Ferguson (1990). Using a discursive orientation involves two main tasks - an explanation of how 'UA' has gained some prominence and the man~er in which its currency is able to be maintained. The investigation included an in-depth analysis of the literature and empirical research in th!"e~Eastern Cape centres. The function of the empirical findings is to expose the discourse, as well as to extend the empirical base relating to 'urban agricultural' research in general. The thesis suggests that the growth in interest in 'UA' is a result of a Foucaultian "gap" opening in the discourse. Changes in the conceptualisation of development, the rise-in prominence of the urban poor and emerging ecodevelopment views, among others, have given proponents of "UA' greater room for manoeuvre. However, most case studies promote 'UA' despite providing little evidence of its role in household welfare. The way the discourse maintains its' currency is in the manner in which the objects of 'UA' are constituted. Firstly, the people are characterised as poor, largely divorced from their rural origins, and mainly female. Each of these assertions is questioned on the basis of the literature itself and the empirical findings. Secondly, in the practice of cultivation, emphasis is placed on the inputs used, and the crops that are produced. The treatment of both the inputs and the crops by the literature is cursory. Investigations show that land availability, garden size and irrigation water are e~pecially problematic and vegetable consumption remains low. Four types of alleged benefit associated with 'UA' are analysed - environmental and ecological, psychological and social, financial, and nutritional. In each case commonly held assumptions are undermined by closer investigation. Thus, the thesis contends that the role played by cultivation is a modest one. Most of the claims made on behalf of 'UA' are more congruent with development discourse than actual cultivation practice.
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- Date Issued: 1997
Theories of exchange rates and the methodology of economics
- Authors: Hodge, Duncan
- Date: 1997
- Subjects: Foreign exchange rates Economics -- Methodology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1012 , http://hdl.handle.net/10962/d1002747
- Description: This thesis is an exercise in applied methodology. Ideas in the history and philosophy of science which have proved to be influential in the methodology of economics, and in shaping economists' self-image in this regard, are selected for closer analysis and criticism. The main ideas that are addressed are those of empiricism, with emphasis on the methodological falsificationism of Karl Popper and Imre Lakatos, and Laudan's problem solving model of scientific progress . The thesis examines the relationship between empirical evidence, in the form of both econometric test results and stylized facts, and the development of theories about exchange rates and the open economy. This analysis begins with Cassel's formulation of purchasing power parity theory in 1916, through the elasticities, absorption, and Mundell-Fleming models of exchange rates and the balance of payments, up to the present day monetary and asset market models. This is done with regard to the broad methodological issues examined earlier in the thesis. Some of the main empirical and methodological difficulties in testing such theories are addressed, with particular reference to the role played by the Duhem-Quine thesis and the ceteris paribus assumption. Although some of these difficulties may be regarded as a matter of degree compared to similar problems in the natural sciences, it is argued that this difference is significant for the workability of falsification in economics . Moreover, the presence of hypotheses about expectations in many economic theories appears to be a substantive difference such that the difficulties posed by the Duhem-Quine thesis apply with much greater force in a social science like economics. The main conclusions are that neither the Popperian nor Lakatosian versions of falsification are seriously practiced in the area of exchange rate economics and that, unlike the position taken by advocates of falsification such as Mark Blaug, it would be inappropriate and misguided to do so. A tentative case is made, with some reference to the theories surveyed in this thesis, for the possibly greater relevance of Laudan' s more pragmatic problem solving model for the methodology of economics, particularly as regards his analysis and emphasis on conceptual problem solving in the progress of knowledge.
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- Date Issued: 1997
The oral-style South African short story in English A.W. Drayson to H.C. Bosman
- Authors: MacKenzie, Craig
- Date: 1997
- Subjects: Short stories, South African (English) -- History and criticism
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2228 , http://hdl.handle.net/10962/d1002271
- Description: This study is concerned with a particular kind of short story in South African English literature - a kind of story variously called the fireside tale, tall tale, yarn, skaz narrative, frame narrative, or (the term used in this study), the 'oral-sty Ie story.' This kind of story is characterised by the use of an internal narrator (a fictional narrator or storyteller figure), the cadences of his or her speaking voice, and a 'reporting' frame narrator. Stories by A. W. Drayson, Frederick Boyle, J. Forsyth Ingram, W. C. Scully, Percy FitzPatrick, Ernest Glanville, Perceval Gibbon, Francis Carey Slater, Pauline Smith, Aegidius Jean Blignaut and Herman Charles Bosman form the principal body of primary sources examined in this study. The Bakhtinian notion of "simple" and "parodistic" skaz narratives is deployed to analyse the increasing complexity to be discerned in the works by these writers, which roughly span the 100 years from the middle of the nineteenth century to the middle of the present century. A "simple" use of the skaz narrative is evident in the early or 'ur-South African' oral-style story, represented here by Drayson, Boyle and Ingram. With Scully and FitzPatrick the form is still used 'artlessly,' although the beginnings of a greater self-consciousness can be discerned. The' Abe Pike' tales by Glanville introduce a more complex use of the fictional narrator, a process taken a step further by Gibbon in his 'Vrouw Grobelaar' tales. With the latter, in particular, the complex or "parodistic" skaz narrative makes its advent in South African literature. The oral-style stories of Slater and Smith are largely a regression to the ear lier form, although there are aspects of their stories which anticipate Bosman. With Blignaut and Bosman, however, the South African oral-style story comes into its own. In their Hottentot Ruiter and Oom Schalk Lourens characters is invested all the complexity and 'double-voicedness' that was latent, and largely dormant, in the earlier oral-style narratives. Through Blignaut, and Bosman in particular, the South African oral-style story achieves its most economical, sophisticated and successful form of expression. The study concludes by looking briefly at the use of an oral style in short stories by black South African writers and argues that their stories are not, formally speaking, to be categorised alongside those by the other~ writers examined. The oral-style story, the study concludes, achieved its apogee in Bosman's Oom Schalk Lourens sequence and went into sharp decline after Bosman's death in 1951.
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- Date Issued: 1997
The effect of elevated CO₂ on Phaseolus vulgaris L. cv Contender
- Authors: Mjwara, Jabulani Michael
- Date: 1997
- Subjects: Plants -- Effect of carbon dioxide on Kidney bean
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4215 , http://hdl.handle.net/10962/d1003784
- Description: The response of Phaseolus vulgaris L. cv. Contender grown in controlled environmental conditions, at either ambient or elevated (360 and 700 μmol mol ̄¹, respectively) CO₂ concentrations ([CO₂]), was monitored from 10 days after germination (DAG) until the onset of senescence. Elevated CO₂ had a pronounced effect on total plant height (TPH), leaf area (LA), dry weight (DW) accumulation and specific leaf area (SLA). All of these were significantly increased by elevated [CO₂] with the exception of SLA, which was significantly reduced. Except for higher initial relative growth rates (RGR) in CO₂-enriched plants, RGR did not differ significantly between the two CO₂ treatments throughout the remainder of growth period. While growth parameters clearly differed between CO₂ treatments, the effects of CO₂ on many physiological processes including net assimilation rate (NAR), Rubisco activity, and some foliar nutrient concentrations were largely transient. For example, CO₂ enrichment significantly increased NAR, but from 20 DAG onward, NAR declined to levels measured on plants grown under ambient CO₂. Similarly, the decline in both foliar N concentration and Rubisco activity in CO₂-enriched plants after 20 DAG was significantly greater than the decline observed for ambient CO₂ plants. Soluble leaf protein and total chlorophylls (a+b) were also significantly reduced in plants grown under elevated CO₂. Chlorophyll (a/b) ratios increased with time underelevated CO₂, indicating that the rate of decline of chlorophyll b was higher than that of chorophyll α. No significant changes in total carotenoid (x+c) levels were observed in either CO₂ treatment. Under enhanced CO₂, the foliar concentrations of K and Mn were increased significantly, while P, Ca, Fe and Zn were reduced significantly. However, changes in Mg and Cu concentrations were not significant. High CO₂-grown plants also exhibited pronounced leaf discoloration or chlorosis, coupled with a significant reduction in leaf longevity. The levels of non-structural carbohydrates (sucrose, glucose, fructose and starch) and nitrogenous compounds (nitrogen, total soluble proteins and free amino acids) were determined for leaves and developing seeds of P. vulgaris. Leaf tissue of elevated CO₂-grown plants accumulated significantly higher levels of both soluble sugars and starch. Leaf ultrastructure revealed considerable erilargement of starch grain sizes with surface areas more than five times larger compared to those of control plants. No apparent differences in structure and membrane integrity of chloroplasts in both CO₂ treatments were noted. Although ambient CO₂-grown plants had comparatively low levels of non-structural carbohydrates (NSC), they accumulated significantly higher levels of nitrogenous compounds. The levels of NSC were consistently higher in seeds of plants grown under elevated CO₂. In comparison to plants grown at elevated [CO₂], pods and seeds of ambient CO₂-grown plants had significantly larger pools of free amino compounds and N. Stomatal conductance (gs) declined significantly, as expected for plants grown under elevated CO₂. This was accompanied by a decline in transpiration rates (E). Reduced gs and E led to high AlE ratio, which meant improved water use efficiency (WUE) values for CO₂-enriched bean plants. Leaf carbon isotope discrimination (∆) against the heavier isotope of carbon (¹³C), has been used to select for high WUE in C₃ plants. In plants grown at elevated CO₂ concentration, ,1 was significantly reduced. Although ∆ was negatively correlated with WUE in both CO₂ treatments, the correlation was steeper and highly negative for CO₂-enriched plants. These results indicate underlying differences in gas-exchange physiology, including stomatal responses between ambient and elevated CO₂-grown plants. Photosynthetic acclimation was investigated using the response of assimilation to internal carbon dioxide concentration (A/C₁ curves). At early stages of growth, the initial slope of the A/C₁ response curve did not differ with CO₂ treatment. In contrast, CO₂-saturated photosynthetic rate (Amax) was significantly higher in plants grown under elevated versus ambient CO₂ at 15 DAG. However, at subsequent stages of growth both the initial slope and Amax declined in bean plants grown in elevated CO₂. Apparent carboxylation efficiency (ACE, estimated from the initial slope of A/C₁ response) values followed a similar trend and were significantly reduced in CO₂-enriched plants. These results indicate that acclimation or negative adjustment of photosynthesis may have been caused by a combination of both stomatal and biochemical limitations. Bean plants grown under conditions of elevated atmospheric CO₂ flowered 3 to 4 days earlier, and produced significantly more flowers and pods than plants grown at ambient conditions. Plants grown at elevated CO₂ aborted 22 and 20% more flowers and pods, respectively, than plants grown at ambient CO₂. Elevated CO₂ also significantly increased the number of tillers or lateral branches produced by plants, which contributed to a significant increase in pod number and seed yield in these plants. Although plants grown at elevated CO₂ produced on average 8 seeds per pod, while plants grown under ambient CO2 conditions produced 5 seeds per pod, the greater number of seeds was offset by lower seed weights in plants grown under _ elevated CO₂. Thus, despite high seed yield in beans grown under elevated CO₂, the harvest index (HI) did not change significantly between CO₂ treatments.
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- Date Issued: 1997
Termination of psychotherapy : a phenomenological exploration of the therapist's and the patient's experience
- Authors: Schlodder, Michelle Ingrid
- Date: 1997
- Subjects: Psychotherapy -- termination Psychotherapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3049 , http://hdl.handle.net/10962/d1002558
- Full Text:
- Date Issued: 1997
Synthetic and physical organic studies of chromone derivatives
- Authors: Ramaite, Ipfani David Isaiah
- Date: 1997
- Subjects: Benzopyrans Heterocyclic compounds -- Derivatives Coumarins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4380 , http://hdl.handle.net/10962/d1005045
- Description: A range of chromone-2-carboxylic acids has been prepared by condensing suitably substituted 2-hydroxyacetophenones with diethyl oxalate. pK₂ Studies of these acids revealed that 6- or 7-methoxy substituents decreased acidity while the 6-nitro group enhanced acidity; the strongest acid was the 3-chloro derivative, the increase in acidity being attributed to steric inhibition of acid-weakening delocalisation between the carboxyl group and the chromone system. Various chromone-2-carboxamides, derived from acid chloride precursors, were converted to polysubstituted acrylamides by nucleophilic ring-opening with selected amine nucleophiles. The main fragmentation patterns exhibited by these acrylamides were elucidated using a combination of low resolution, high resolution and meta-stable peak analysis, while the effect of substituents on the simultaneous internal rotation involving the carboxamide and enamine moieties were studied using dynamic NMR spectroscopy. Rotational barriers of ca. 67.1 kJmol ̄¹ and ca. 102 kJmol ̄¹ were found for the enamine and amide rotors, respectively. Several synthetic pathways were followed to prepare a series of 2-(N,N-dialkylamino)chromones which were subjected to detailed mass spectral analysis. In addition to substituent-specific fragmentations , the 2-aminochromones appear to fragment via 3 major pathways. The effect of substituents on the internal rotation of the amino moeity was investigated by variable temperature ¹H NMR spectroscopy and the resulting DNMR data was used to calculate the rotational barriers. Examination of the data reveals that the electron-releasing 6- and 7- substituents reduce the C-NMe₂ rotational barrier to ca. 43.5 kJmol ̄¹ , while the nitro analogue has the largest rotational barrier (ca. 46.1 kJmol ̄¹) because of the electron-withdrawing effect of this substituent.
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- Date Issued: 1997
Studies of the biology and ecology of the high shore South African limpet, Helcion pectunculus (Mollusca : patellogastropoda)
- Authors: Gray, David Richard
- Date: 1997
- Subjects: Mollusks -- South Africa Limpets -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5776 , http://hdl.handle.net/10962/d1005464
- Description: Investigations were carried out into aspects of the bio!ogy and ecology of Helcion pectunculus along the coait of South Africa. These included studies of the distribution, density and biomass of the limpet at six sites along the east coast plus one site on the west coast; the growth of H pectunculus on both west and east coasts; a comparison of the reproductive biology of the east and west coast populations; the foraging activity and feeding behaviour of H pectunculus and the driving forces behind the rhythmic behaviour of this limpet; the importance of the crevice environment in the biology and ecology of H pectunculus. Helcion pectunculus has a restricted zonation, with the majority of animals residing in crevices in the upper Balanoid zone during diurnallowtides, although individuals were occasionally found in the lower Balanoid zone on shores with a gently sloping aspect. This limpet occurs in higher densities (50-lO0 individuals/m2) on shores which have large numbers of crevices and boulders i.e. quartzitic sandstone shores. On most shores, the ratio of males to females differed significantly from a 1: 1 ratio with the highest ratio being obtained on the west .. coast (3 males: 1 female). At all sites, the populations of H pectunculus exhibited strong sexual dimorphism. Males and females were always found to differ in size, with individuals of < 20 mm shell length generally being male whilst limpets with a shell length of> 22 mm were generally female. Helcion pectunculus grows allometrically, increasing in height faster than length, which is expected of a high shore gastropod mollusc attempting to reduce evaporative water loss. Growth rates were similar on both east and west coasts regardless of the differing oceanographic conditions. The theoretical values of Lmax were also similar being 30.86 mm and 30.71 mm respectively. Micro-growth bands are laid down within its' shell which have the same periodicity as the tidal cycle and these enabled age estimates to be made. Younger individuals were male whilst older animals were female, suggesting that H pectunculus is a protandrous hermaphrodite. Histological examination proved, unequivocally, that this limpet undergoes a protandric sex change, changing from male to female when they are about 2 years old. Both east and west coast popUlations had a marked reproductive cycle, exhibiting two spawning periods a year, one in April and another in November. The possibility that the reproductive pattern exhibited is now phylogeneticallyconstrained is discussed. It is suggested that H pectunculus has evolved a reproductive cycle which will allow its planktonic larvae to utilise the valuable phytoplankton bloom food source whilst using onshore winds to ensure that larvae are not transported out to sea and lost. The number of foraging excursions carried out by individuals of H pectunculus was found to have a significant effect on Gonad Index and hence potential reproductive output. The activity pattern of H pectunculus varied depending upon micro-habitat; animals inhabiting both east and west facing rock surfaces are active during nocturnal low tides whilst animals on west facing rock surfaces are also active during daytime low tides whilst in the shade. Limpets travel further during foraging excursions in winter (X = 85.53 cm) than in either spring (x = 55.7 cm) or summer (X = 48.8 cm) and also during spring low tides (x = 89.8 cm) compared with neaps (x = 40.9 cm). This limpet exhibits rigid homing to a fixed scar within a-crevice and feeding excursions were found to cons.is.t. of three distinct phases, a rapid outward phase, a slower foraging phase and a rapid homeward phase. Foraging was always highly directional, with a mean vector which took limpets onto an area of the rockface with the highest microalgal biomass and also the smoothest rock surface. Helcion pectunculus exhibits a free-running endogenous rhythm of locomotor activity with both circadian and circatidal components and it is suggested that this rhythm plays a role in allowing the limpet to avoid unfavourable environmental conditions. The exogenous entrainment factor of this endogenous rhythm was the time of exposure to air in the field. There was found to be an organized distribution of limpets within crevices with smaller, younger limpets being towards the back of the crevice and larger, older limpets towards the crevice mouth. It is hypothesized that juvenile limpets of this species actively select and settle at the backs of crevices responding to chemical cues of adult conspecifics. The crevice refuge supplies the limpets with a stable and buffered environment with higher relative humidities (X = 72.3%) and lower rock surface temperatures (X = 19.7°C) than adjacent exposed rock surfaces (X = 64.5%; x = 22.9°C). Limpet body temperatures were significantly lower in crevic~refuges compared to limpets on exposed rock surfaces. Body temperatures never exceeded the rock surface temperatures. It is suggested that this is the result of morphological adaptations such as shell ornamentation and allometric growth. Light levels above 1000 J.1E.m-2.s-1 inhibited foraging activity in H. pectunculuswhilst limpets subjected to 30-50% shade foraged even during daytime lowtides. This limpet is one of the least tenacious (2.75 ± 0.13 kg.cm-2 ) of all South African limpets and the possibility that wave activity governs both the activity patterns and homing behaviour of this limpet is discussed. Limpets deprived of a crevice refuge experienced extremely high mortalities, with 45% of the limpets being lost during the first high tide period. A hypothetical model of the hierarchy of exogenous factors controlling limpet foraging activity is introduced and discussed in relation to the results of this study. Finally, it is suggested that in addition to the "migratory" and "non-migratory" groups of limpets present on southern African shores a third group of limpets seem to be present which may be classed as "specialized non-migratory" species. These are species that do not migrate, garden or aggressively fight off like conspecifics. They have overcome the competition for space and food on intertidal rocky shores by adapting to a particular habitat which is exclusive to them alone. From the combined results of these studies, it can be stated that H. pectunculus has adapted physiologically, morphologically and behaviourally to successfully survive the extreme conditions in the upper Balanoid zone.
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- Date Issued: 1997
Solid state thermal decomposition of amide complexes of nickel(II) chloride
- Authors: Nelwamondo, Aubrey Ndifelani
- Date: 1997
- Subjects: Decomposition (Chemistry) Materials -- Thermal properties Amides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4345 , http://hdl.handle.net/10962/d1005008
- Description: The thermal decompositions of a series of amide complexes of nickel(II) chloride have been studied. Thermochemical, kinetic, structure and solid-state stability correlations have been investigated. Complexes containing homologous amide ligands (L) of the form NiLCℓ₂, Ni₃L₂Cℓ₆, Ni₃LCℓ₆, NiL₂Cℓ₂(2H₂0) and ML₂Cℓ₂ (where M = Ni(II), Co(II) or Cu(II)) have been prepared. Chemical analysis, spectral and thermogravimetric measurements were used to characterize the complexes and their decomposition stoichiometries. Three sets of reactions were identified as yielding stable products in a single step: (i) NiLCℓ₂ (s) → NiCℓ₂ (s) + L (g) (ii) Ni₃LCℓ₆ (s) → 3NiCℓ₂ (s) + 2L (g) (iii) Ni₃LCℓ₆ (s) → 3NiCℓ₂ (s) + L (g) Characterization of the processes in the ML₂Cℓ₂ and NiL₂Cℓ₂(2H₂0) complexes was not straightforward. Reaction enthalpies (ΔH) were determined using DSC. The orders of the reaction onset temperatures (Tc), peak temperatures (Tmax) and ΔHL values for the NiCℓ₂ system were: N-methylacetamide < acetamide < N-methylformamide, suggesting the importance of steric hindrance of the methyl-substituent groups in the amide skeleton. In the Ni₃LCℓ₆, NiL₂Cℓ₂(2H₂0) and ML₂Cℓ₂ systems no simple orders could be deduced. The Te and Tmax sequences obtained from analogous metal(II) chloride complexes indicated that the copper(II) complexes were the least stable. The kinetics of the loss of L from NiLCℓ₂ complexes were investigated using isothermal TG, non-isothermal TG and DSC measurements. The contracting geometry models described the course of the decompositions in the most satisfactory manner. Apparent activation energies ( Ea) were estimated from Arrhenius plots of rate coefficients from: (i) an approximate zero-order relationship, (ii) the contracting-area (R2) and contracting-volume (R3) equations, (iii) a new empirical (B2) expression, (iv) the half-life ( 1/t₀.₅) and (v) the characteristic feature of the rate-time curve ( 1/tmax/2 ). The non-dependence of Ea on the rate equation used supports the reliability of the kinetic parameters. Non-isothermal experiments were analyzed by the Coats-Redfern, the modified BorchardtDaniels and the Kissinger methods. Arrhenius parameters were in keeping with results from the isothermal kinetic measurements. The values of Ea obtained for the NiLCℓ₂ system increased with an increase in basicity of the amide ligands. No straightforward correlation was found between Ea and Te, Tmax, ΔHL or spectral properties.
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- Date Issued: 1997
Share-net: a case study of environmental education resource material development in a risk society
- Authors: Taylor, Robert James
- Date: 1997
- Subjects: Environmental education Environmental education -- South Africa Environmental education -- Activity programs -- South Africa Environmental education -- South Africa -- Information resources
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1765 , http://hdl.handle.net/10962/d1003650
- Description: This narrative study reviews the development of Share-Net, an informal resource materials network, located within the developing environmental education activities of the Wildlife and Environment Society of South Africa. Historical shaping factors within the Society are discussed and changing views on conservation, environmental education and research are described. Rather than the research process being a utilitarian and outside endeavour for clarifying and monitoring, the research orientation of this study is one of grounded activity within and integrally part of processes of change. Within a developing story of resource materials and workshops, themes are traced and emergent tensions are critically reviewed. Teacher workshops from 1981 until 1995 are analysed and developing orientations are described. Within this review changes are evident from an orientation of 'us' informing 'them' to joint, collaborative endeavours within the development and use of resource materials. The study reviews and illuminates the Share-Net project around questions of project orientation and management and this is done through the narration of case studies. Principles and patterns emerging within these are examined to guide future resource development projects. Popularist notions of networking and structural functionalist notions of social change are also examined. Grand, modernist strategies designed to cause change in others are questioned and the role of small-scale nodes of resource material activity cooperating within an open network are clarified as useful alternatives. Local resourcing centres such as these have proved useful in supporting teachers in the development, use and adaptation of resource materials. The study also examines income and expenditure of the Share-Net project since 1988 to review economic sustainability. A case is made for sustaining the project conceptually and financially through the sales of products and services rather than through external donor sources. Finally the study raises questions and challenges within the project and proposes guiding frameworks for future review, in action, as the story continues.
- Full Text:
- Date Issued: 1997
Regulation of tryptophan-2,3-dioxygenase and pineal indoleamines by selected tryptophan derivatives and antidepressants
- Authors: Walsh, Harold Archibold
- Date: 1997
- Subjects: Antidepressants Tryptophan -- Physiological effect Tryptophan -- Therapeutic use Depression, Mental -- Treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4017 , http://hdl.handle.net/10962/d1004077
- Description: The regulation of tryptophan-2,3-dioxygenase (TDO) (EC 1.13.1.12) and, to a lesser extent, pineal indoleamines, both in vitro and in vivo, is examined in this study. Rat liver TDO is a cytosolic enzyme which plays a crucial role in the regulation of circulating tryptophan (TRP) levels. Stimulation of this enzyme by heme enhances the catabolism of TRP, making less TRP available for uptake into the brain and other tissues, and for protein synthesis. At pH 7, the enzyme has an approximate Km of 100μM, is subject to substrate inhibition immediately beyond Sopt([S] at Vmax), and response of the enzyme is cooperative in both uninhibited and inhibited regions. Hill analysis of the uninhibited region reveals a biphasic plot and two classes of binding sites. Negative cooperativity is brought about through deprotonation of the enzyme. Substrate iphibition also occurs at both acidic and basic pH values with concomitant shifts in Sopt. The results obtained indicate that substrate inhibition could be an additional mechanism whereby the flux through the TRP-kynurenine pathway is regulated. TDO is subject to a diurnal rhythm, with peak activity during the pre-dark period and the loweSt activity towards the end of the dark period. It is possible that the enzyme controls the synthesis of the neurotransmitter serotonin (5-HT), and that the circadian rhythm in TDO activity is due to the endogenous rhythm of melatonin (aMT) production by the pineal gland. In the present study, aMT displaces TRP from bovine serum albumin (BSA) in vitro, and it is therefore possible for the indoleamine to regulate the availability of TRP for uptake into the brain for conversion to its derivatives. Chronic intraperitoneal administration of aMT affects physiological hepatic parameters in rats, such as TDO activity and stromal fatty acid composition, whilst no observable effect is demonstrable with respect to protein synthesis, nucleic acid metabolism, membrane fatty acid composition and pineal indole biosynthesis. On the other hand, chronic treatment of rats with antidepressants, the tricyclic desmethylimipramine (DMI) and the selective serotonin reuptake inhibitor (SSRI), fluoxetine, reveals significant negative alterations in TDO concentrations and pineal indole amine synthesis. Combining aMT with any of these two drugs normalises the activity of the hepatic enzyme. DMI is found to be an effective inhibitor of TDO in the micromolar range in vitro, and also affects total enzyme concentrations in vivo. Fluoxetine has no effect on TDO in vitro, but in vivo also reduces total enzyme levels in the liver. However, the SSRI does not affect conjugation between apo- and holoenzyme. Instead, it decreases extant holoenzyme levels. Indoleamine synthesis by the pineal gland, in organ culture, is altered by both antidepressants, although in different ways. DMI increases N-acetylserotonin levels and reduces the output of methoxyindole acetic acid and meth6xytryptophol. Fluoxetine treatment markedly reduces aMT concentrations and also brings about high levels of the 5-HT catabolites, 5-hydroxytryptophol and 5-hydroxyindole acetic acid. Insulin also lowers aMT synthesis significantly in pineal organ cultures, via a mechamsm that involves inhibition of the enzyme, N-acetyl transferase, that regulates aMT synthesis. The effects of insulin on pineal indole metabolism are due to the observation that a carbohydrate rich diet which induces insulin release elevates plasma TRP and brain 5-HT, but has no effect on pineal TRP and indole amine synthesis. It could thus be possible for insulin to have an effect on the pineal, since the latter is outside the blood brain barrier. The finilings of this study support the biogenic amine deficiency hypothesis, implicating some of the major biogenic amines such as noradrenaline (NA), 5-HT and aMT in depression. There is believed to be a deficiency of NA and 5-HT at their respective synapses in the depressed state. The drug DMI could act, firstly, by inhibiting TDO and thus increasing plasma TRP levels, and could, secondly, stimulate NA release and inhibit NA reuptake at the pineal membrane. The combined effect would be to enhance aMT synthesis, with eventual remission. Fluoxetine, on the other hand, appears to utilize a slightly different mode of action to DMI, which seems to focus on the preservation of 5-HT. The fact that aMT counteracts the effects of both antidepressants, and restores the activity of TDO to that of the controls, is also consistent with the observation that the therapeutic action of drugs such as these coincides willi the restoration of normal plasma levels of the neurohormone in depressives. In view of the biogenic amine deficiency hypothesis of depression and the contentious claim that TDO is the major peripheral determinant of brain TRP, brain 5-HT and ultimately aMT, the regulation of TDO is investigated and discussed. The study concludes that TDO activity is regulated by a number of endogenous compounds which are mainly derivatives of TRP, such as aMT and oxidized nicotinamide adenine dinucleotide and exogenous substances, of which DMI and fluoxetine are but two. In addition, modulation of IDO activity in depression appears to be an important aspect of antidepressant action. aMT, the product of the pineal gland, also has the potential to increase plasma TRP and hence forebrain TRP levels, and ultimately 5-HT concentrations, firstly by displacing TRP from serum albumin and secondly by inhibiting TDO.
- Full Text:
- Date Issued: 1997
Regional and local economic development strategies in the Eastern Cape and guidelines for future development
- Authors: Nel, E L
- Date: 1997
- Subjects: Community development -- South Africa -- Eastern Cape Case studies Community development Community development -- South Africa -- Eastern Cape Eastern Cape (South Africa) -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4848 , http://hdl.handle.net/10962/d1005524
- Description: Local Economic Development (LED) is an applied economic development strategy which seeks to address site-specific needs through locally appropriate solutions. In this thesis, the faith being accorded to the potential of LED in South Africa is critically examined. The study is based on a detailed examination of the experience of regional development and several emerging cases of LED in the Eastern Cape Province of South Africa. High levels of poverty and unemployment necessitate and justify innovative approaches to address such problems. The thesis examines the potential of LED strategies and identifies applied policy guidelines which can help address the Province's development needs. The theoretical framework of this research is based on a critical examination of international and South African literature dealing with development issues, LED and localities theory. A detailed documentary examination of early LED endeavours and the experience of regional development in the present century was undertaken. Results suggest that LED is not a new concept and that regional development, through its political bias, achieved only limited success. Contemporary examples of LED were identified and examined through detailed site-specific investigations. This was carried out through the use of semistructured interviews supplemented by participatory rural appraisal and questionnaire surveys which served as a means to triangulate the results. The research findings indicate that regional development, as applied in the study area, did not lead to the establishment of a permanent industrial base. In terms of the concept of LED, it is apparent that it has allowed for socio-political empowerment but has only improved economic conditions in the more well-endowed case-studies. In these areas, positive antecedent conditions and the key role played by community supportive nongovernmental organizations is apparent. Despite the limited degree of success which many initiatives attain, the thesis regards LED as a development alternative for areas which are unlikely to draw in external investment. Some of the key contributions of the thesis include the advancement of a refined typology of LED, the development of a research schedule to investigate and assess LED initiatives and the postulation of appropriate development guidelines and theoretical constructs.
- Full Text:
- Date Issued: 1997
Radio observation of the Gum Nebula Region
- Authors: Woermann, Beate
- Date: 1997
- Subjects: Radio sources (Astronomy) Nebulae -- Observations Radio astronomy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5485 , http://hdl.handle.net/10962/d1005271
- Description: This thesis describes the results of an investigation of the physical properties of the Gum Nebula. For this investigation a radio continuum map of the region was made at 2326 MHz and resolution ⅓° with the HartRAO antenna. This map was used to generate spectral index images and an infrared to radio flux density ratio (IRR) image. The latter image shows that the IRR of the nebula is in the range 20 to 250, identifying it as an old SNR. Several spectral index images of this region were generated using two different methods, one based on the isolation of the nebula from its background radiation, the other based on TT-plots (Turtle et al., 1962). The two methods yield similar results, which show that the nebula has a thermal shell with a non-thermal region in its interior. Below the galactic plane the thermal region dominates and above the plane the nonthermal region. These results suggest a model of an old SNR with an H II region shell. Spectral line observations of hydrogen recombination lines and hydroxyl (OH) were made with the HartRAO and the Mopra telescopes. The detection of hydrogen recombination lines at four positions in the thermal regions of the nebula give electron temperatures and emission measures in the ranges 4000 to 6000 K and 220 to 460 pc.cm⁻⁶ respectively. The turbulent velocities are of the order of 20 km/s. A search for shocked OH lines at 1667 MHz and 1720 MHz in the Gum Nebula gave results that were negative, but numerous unshocked 1667 MHz OH lines were detected. The latter were used in a test for an expansion of the nebula. The most plausible fit to the data gives an expansion centre at l = 260.5°, b = -2.5° and at a distance of 0.7 kpc from us. The front face angular radius and expansion velocity are 10.5° and 16 km/s respectively. The back face angular radius and expansion velocity are 8.50 and 7 km/s respectively.
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- Date Issued: 1997
Parasitism and invasive species : an ecological study of mussel populations
- Authors: Calvo Ugarteburu, Miren Gurutze
- Date: 1997
- Subjects: Perna -- Parasites Mytilidae -- Parasites Perna -- Ecology Mytilidae -- Ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5736 , http://hdl.handle.net/10962/d1005422
- Description: The Mediterranean mussel Mytilus galloprovincialis, was introduced accidentally to South Africa and has since become invasive. One possible explanation for the success of this species is that it has been released from the effects of parasites which affect the indigenous species of mussels. The aim of this work was to examine the parasitic load of different mussel populations along the southern African coast and to assess the effects of parasites on their hosts. A survey was done to quantify the levels of parasitism in indigenous populations of Perna perna from Southern Africa and of Mytilus galloprovincialis from South Africa (where it is exotic) and Spain (where it is indigenous). This survey was carried out at three different geographic scales: small scale (metres), studying the incidence of parasites within a mussel bed; medium scale (kilometres to tens of kilometres), comparing prevalences of infection among different localities; and large scale (hundreds to thousands of kilometres), examining infection rates along the Southern African coast, as well as the coast of the Basque Country (North Spain). During this survey metazoan parasites other than trematodes were rare. Four species of trematodes were commonly found infecting the indigenous mussel Perna perna. These were metacercariae of the genus Proctoeces, bucephalid sporocysts, encysted metacercariae on the labial palps and gelatinous cysts with metacercariae inside. A detailed description of Proctoeces is given; the other parasites are described elsewhere. No parasites were found in M. galloprovincialis either in South Africa or in Spain. This thesis concentrates on the study of prevalences of Proctoeces and bucephalid sporocysts and their effects on Perna perna. Infection rates with Proctoeces are highly dependent on the sex of the host, with more females than males being infected, and are also size dependent, though only for females. Identification of the sex of mussels infected with bucephalid sporocysts is often not possible since the sporocysts spread over the gonad and replace it. Prevalence of infection with this parasite also increases with the size of the host. To study the influence of Proctoeces and bucephalid sporocysts on the ecological fitness of Perna perna, their effects on survival and competitive ability were tested. The results showed significant negative effects. Both parasites significantly depressed condition but only after spawning, when the mussels were already stressed. In order to check for effects on host survival, the effects of both parasites on mortality rates, gaping behaviour and water loss of mussels exposed to air were also examined. Neither parasite affected mortality rate or gaping behaviour of Perna perna. Proctoeces did not affect the amount of water lost by mussels, but the bucephalid sporocysts did. Mussels infected with sporocysts lost significantly more water than non-infected individuals. This increase in water loss was not related to the gaping behaviour, but a test of the strength of the adductor muscles showed that less force was needed to open mussels with bucephalid sporocysts than non-infected mussels. This was not the case for mussels infected by Proctoeces. Weaker mussels will fail to seal the valves properly, resulting in an increase of water loss on exposure to air by evaporation. Another factor that will have an obvious effect on a population is the reproductive output of the animals. Histological sections of the gonad of infected and non-infected females were cut to study the effects of both parasites on reproduction. Statistical tests comparing the numbers and sizes of oocytes in females infected with Proctoeces and non-infected females showed no significant differences. However, bucephalid sporocysts have a dramatic effect on reproduction by castrating the host, leaving no trace of sex products. One of the major factors shaping the composition of a mussel bed is competition for space and food, with smaller mussels being at a competitive disadvantage. Thus, in order to examine effects of both parasites on the competitive ability of Perna perna, summer and winter growth rates were compared for infected and non-infected mussels. Proctoeces reduced growth both in summer and in winter whilst bucephalid sporocysts had no significant effect. Both growth and reproduction are important components of the energy budget of an animal, and each is affected by either Proctoeces or the bucephalid sporocysts. In an attempt to test if Perna perna compensates energetically for these negative effects, filtration rates and oxygen consumption of mussels with and without parasites were measured. Neither parasite had a significant effect on filtration rates or oxygen consumption of the host. All these results indicate that both Proctoeces and the bucephalid sporocysts have a detrimental effect on their host, and that the mussels do not compensate for these negative effects. There is neither an increase in filtration, nor a decrease in respiration to balance the energy lost to the parasite. The two parasites studied affect the host in different but complementary ways. The effects of both parasites are concentrated on those size classes of mussel which channel most energy into the portion of the energy budget affected by the parasite. Proctoeces affects growth only in the smaller individuals, which under normal conditions would put most energy into growth; and the bucephalid sporocysts castrate the bigger mussels, which would expend most energy on reproduction. By reducing growth rates of small mussels or castrating large mussels, these parasites effectively remove them from the breeding population and reduce their competitive abilities. These negative effects, together with the high prevalence of both parasites in Perna perna along the South African coast and their absence in Mytilus galloprovincialis, suggest that parasites may be an important reason for the success of Mytilus.
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- Date Issued: 1997
Modelling parallel and distributed virtual reality systems for performance analysis and comparison
- Authors: Bangay, Shaun Douglas
- Date: 1997
- Subjects: Virtual reality Computer simulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4657 , http://hdl.handle.net/10962/d1006656
- Description: Most Virtual Reality systems employ some form of parallel processing, making use of multiple processors which are often distributed over large areas geographically, and which communicate via various forms of message passing. The approaches to parallel decomposition differ for each system, as do the performance implications of each approach. Previous comparisons have only identified and categorized the different approaches. None have examined the performance issues involved in the different parallel decompositions. Performance measurement for a Virtual Reality system differs from that of other parallel systems in that some measure of the delays involved with the interaction of the separate components is required, in addition to the measure of the throughput of the system. Existing performance analysis approaches are typically not well suited to providing both these measures. This thesis describes the development of a performance analysis technique that is able to provide measures of both interaction latency and cycle time for a model of a Virtual Reality system. This technique allows performance measures to be generated as symbolic expressions describing the relationships between the delays in the model. It automatically generates constraint regions, specifying the values of the system parameters for which performance characteristics change. The performance analysis technique shows strong agreement with values measured from implementation of three common decomposition strategies on two message passing architectures. The technique is successfully applied to a range of parallel decomposition strategies found in Parallel and Distributed Virtual Reality systems. For each system, the primary decomposition techniques are isolated and analysed to determine their performance characteristics. This analysis allows a comparison of the various decomposition techniques, and in many cases reveals trends in their behaviour that would have gone unnoticed with alternative analysis techniques. The work described in this thesis supports the Performance Analysis and Comparison of Parallel and Distributed Virtual Reality systems. In addition it acts as a reference, describing the performance characteristics of decomposition strategies used in Virtual Reality systems.
- Full Text:
- Date Issued: 1997
Metabolic responses in melanoma cells to combined nutrient supplementation
- Authors: Midgley, Nicola-Ann
- Date: 1997
- Subjects: Melanoma Tumors -- Growth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4036 , http://hdl.handle.net/10962/d1004096
- Description: This thesis examined the effect and biochemical mechanism by which combined vitamin E and C supplementation may influence tumour cell growth. The study initially addressed the effect of combined vitamin E succinate and Asc supplementation over a nutritional concentration range (5- 20μg/ml) and (25-50μg/ml) respectively, on the in vitro growth of non-malignant LLCMK and malignant BL6 cells. Supplementation of BL6 and LLCMK cells with combined vitamin E succinate and ascorbic acid, resulted in no significant increasing or decreasing trend in LLCMK cell growth, while in BL6 cells a significant decrease in cell growth was observed at all combined vitamin concentrations. It has been suggested that these vitamins may act synergistically to inhibit tumour cell growth through their antioxidant properties in quenching free radicals and lipid peroxidation and furthermore through their modulation of the activities of various enzymes and metabolites in the eicosanoid pathway. This study consequently investigated the effects of combined vitamin E succinate and ascorbic acid supplementation on these parameters. Throughout this study, emphasis was placed on the BL6 melanoma cells, as combined vitamin E succinate and ascorbic acid supplementation did not significantly affect growth or levels of secondary metabolites in the non-malignant LLCMK cells. Combined vitamin E succinate and ascorbic acid supplementation of BL6 cells resulted in a marked but non significant increase in free radical and a significant increase in lipid peroxidation levels. This prooxidant effect was accompanied by a significant decrease in BL6 cell growth, suggesting that the growth inhibitory effects of combined vitainin E succinate and ascorbic acid on BL6 cells in vitro was not mediated through their synergistic antioxidant properties. Vitamin E succinate is a nonphysiological antioxidant in its esterified form, hence cleavage of the succinate group must occur in order for ascorbic acid to interact with the free alcohol, vitamin E. The inability of combined vitamin E succinate and ascorbic acid to reduce free radicals and lipid peroxidation levels within BL6 cells may not be due to their ineffectiveness as antioxidants but rather the presence of other contributing factors which influence the oxidation state within the BL6 cells. Vitamin E is believed to modulate membrane-bound enzymes through membrane stabilization. Furthermore, the stabilizing effect of vitamin E may be enhanced by the ascorbic acid-sparing effect of vitamin E. Hence, this study investigated the effect of combined vitamin E succinate and ascorbic acid in modulating the activity of various enzymes and secondary messengers in the eicosanoid pathway. Supplementation with combined vitamin E succinate (5-20μg/ml) and ascorbic acid (25-50μg/ml) resulted in significant increases in phospholipase A₂, 5-lipoxygenase, cyclooxygenase and adenyl ate cyclase activity, with a significant decrease in BL6 cell growth. The possible synergistic action of these vitamins in terms of modulating membrane-bound enzymes was further substantiated by uptake and cellular distribution studies. Vitamin E succinate and vitamin E in the membrane fraction increased significantly compared to control cultures, while ascorbic acid levels were significantly higher in the stroma fraction when compared to membrane fractions. Consequently, another factor accounting for increased activities of phospholipase A2, 5-lipoxygenase and adenylate cyclase activities as a result of vitamin supplementation in BL6 cells may be an increased availability of Ca²+. Supplementation of BL6 cells with combined vitamin E succinate and ascorbic acid resulted in significant increases in intracellular Ca²+ levels at all combined vitamin groups. Furthermore, this increase in intracellular Ca²+ was positively correlated with cl1anges of the above-mentioned enzyme activities. Within the eicosanoid pathway, the rate of prostaglandin synthesis is regulated by phospholipase A₂ activity and arachidonic acid release, and the net prostaglandin production is dependent on cyclooxygenase activity, hence the effects of combined vitamin E succinate and ascorbic acid on arachidonic acid composition and prostaglandin production within BL6 cells was determined. The percentage arachidonic acid composition of the BL6 cells was elevated and inversely related to cell growth following combined vitamin E succinate and ascorbic acid supplementation. Prostaglandin E₂ and prostaglandin I₂ levels increased significantly, while those of prostaglandin D2 and prostaglandin F₂α increased markedly following supplementation of combined vitamin E succinate and ascorbic acid. These increases in prostaglandin levels were inversely related to BL6 cell growth, suggesting that the prostaglandins were involved in negative regulation of BL6 cell growth. When comparing the levels of prostaglandins, prostaglandin E2 levels were significantly higher when compared to prostaglandin D₂, prostaglandin F₂α and prostaglandin I₂ suggesting that vitamin E₂ succinate and ascorbic acid effects were mediated primarily through an increase in prostaglandin E2. Hence, prostaglandin E2 levels in combined vitamin E succinate and ascorbic acid appeared to be dependent on the amount of precursor present and the activity of its synthetic enzymes. This was confirmed when BL6 cells were supplemented with arachidonic acid. Arachidonic acid had an inhibitory effect on BL6 cell growth and also stimulated prostaglandin E₂ production. Prostaglandin E₂ levels are in turn believed to modulate adenylate cyclase activity in BL6 cells, hence it is reasonable to conclude that adenylate cyclase activity is dependent on prostaglandin E₂ levels. Combined vitamin E succinate and Asc supplementation to BL6 cells resulted in significant increases in adenyl ate cyclase and cyclic adenosine monophosphate, which again correlated with a significant decrease in cell growth. As cyclic adenosine monophosphate has a regulatory role in the cell cycle this study suggested that the effect of combined vitamin E succinate and ascorbic acid supplementation was mediated through the final effect provided by the second messenger, cyclic adenosine monophosphate. This was confirmed when BL6 cells were supplemented with dexamethasone, a phospholipase A₂ inhibitor. This treatment rsulted in combined vitamin E succinate and ascorbic acid having no inhibitory effect on BL6 cell growth. Cyclooxygenase activity, prostaglandin E₂ levels, adenylate cyclase activity and cyclic adenosine monophosphate levels were significantly lower in dexamethasone-treated cells compared to non-treated dexamethasone cultures. The reason for the increased free radical and lipid peroxidation levels in BL6 cells was further investigated. Cyclooxygenase enzymes are believed to generate free radical species during catalytic activity. Analysis of free radical and lipid peroxidation levels following supplementation with dexamethasone revealed markedly lower free radical and significantly lower lipid peroxidation levels in comparison with control cultures and non dexamethasone-treated cultures. These results suggest that the observed increases in free radical and lipid peroxidation levels in BL6 cells supplemented with combined vitamin E succinate and ascorbic acid were indirectly due to the increase in cyclooxygenase activity in these cells.
- Full Text:
- Date Issued: 1997
Healing stories of the unconscious: past-life imagery in transpersonal psychotherapy
- Authors: Knight, Zelda Gillian
- Date: 1997
- Subjects: Transpersonal psychotherapy Unconscious Reincarnation therapy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3004 , http://hdl.handle.net/10962/d1002513
- Description: Theoretically this thesis was grounded in the discourse of transpersonal psychology and the related discourse of transpersonal feminism. The focus was on a particular category of transpersonal phenomena - past-life experiences. These experiences were viewed from a poetic and therapeutic perspective as being healing stories of the unconscious that served to articulate psychological and spiritual realities of the human psyche within both the personal and the collective unconscious. They were thus not questioned in regard to their literal occurrence. The central aims of this thesis were to (a) document and faithfully describe a participant's past-life experiences that occurred during selected psychotherapy sessions, (b) engage in a hermeneutic dialogue between the participant's past-life experiences and contemporary transpersonal literature, and, in so doing, to evaluate and extend existing theory, (c) uncover the archetypal significance of past-life experience and its relationship to the re-emerging Feminine within patriarchal culture and, finally, (d) show how the past-life stories and images contribute to the process of inner healing and transformation, a process termed 'spiritual emergence'. The research was a phenomenological-hermeneutic case study, comprising the selection of eight consecutive psychotherapy sessions in which nine past-life experiences were identified. These sessions were reduced to narrative synopses, and a hermeneutically grounded thematic analysis of a total of six past-life themes were explicated. Principle conclusions reached were that past-life stories and images contribute to the process of spiritual emergence and empowerment as well as to the re-emergence of the Feminine consciousness. Moreover, as healing stories of the unconscious, these past-life experiences can be understood as expressions of the collective struggle with unresolved archetypal forces within the collective psyche, as well as echoes of personal conflicts and dilemmas from the individual unconscious.
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- Date Issued: 1997
Grouping complex systems for classification and parallel simulation
- Authors: Ikram, Ismail Mohamed
- Date: 1997
- Subjects: Digital computer simulation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4662 , http://hdl.handle.net/10962/d1006665
- Description: This thesis is concerned with grouping complex systems by means of concurrent model, in order to aid in (i) formulation of classifications and (ii) induction of parallel simulation programs. It observes, and seeks f~ furmalize _ and then exploit, the strong structural resemblance between complex systems and occam programs. The thesis hypothesizes that groups of complex systems may be discriminated according to shared structural and behavioural characteristics. Such an analysis of the complex systems domain may be performed in the abstract with the aid of a model for capturing interesting features of complex systems. The resulting groups would form a classification of complex systems. An additional hypothesis is that, insofar as the model is able to capture sufficient . programmatic information, these groups may be used to define, automatically, algorithmic skeletons for the concurrent simulation of complex systems. In order to test these hypotheses, a specification model and an accompanying formal notation are developed. The model expresses properties of complex systems in a mixture of object-oriented and process-oriented styles .. The model is then used as the basis for performing both classification and automatic induction of parallel simulation programs. The thesis takes the view that specification models should not be overly complex, especially if the specifications are meant to be executable. Therefore the requirement for explicit consideration of concurrency on the part of specifiers is minimized. The thesis formulates specifications of classes of cellular automata and neural networks according to the proposed model. Procedures for verificati6If - and induction of parallel simulation programs are also included.
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- Date Issued: 1997
Environmental education : a strategy for primary teacher education
- Authors: Wagiet, Razeena
- Date: 1997
- Subjects: Environmental education -- Study and teaching (Elementary) Environmental education -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1512 , http://hdl.handle.net/10962/d1003394
- Description: This research focuses on environmental education in initial teacher education, and is grounded in three interlinked and widely recognised assumptions. First, that education for sustainable living can assist in resolving some environmental problems that are contributing to the environmental crises of sustainability currently facing South Africa and the rest of the world. Second, that education for sustainable living can assist in the establishment of a new environmental ethic that will foster a sustainable way of living. Third, that teacher education is a vital process for the attainment of both. These assumptions inform the aim of this research, which is to explore the potential for the implementation of education for sustainable living, and to identify a strategy for this, for initial teacher education, for senior primary school student teachers in the Western Cape. The strategy is derived following the grounded theory approach, developed through the case study method. In the process of identifying the strategy, this study establishes that there are challenges at macro, meso and micro levels that are obstructing the changes necessary for education for sustainable living. Change theory provides the basis for explaining these shortcomings, by helping to identify the barriers that might obstruct the realisation of the changes that are necessary for education for sustainable living. These challenges need to be perceived in the light of overcoming three sets of barriers in the way of the potential implementation of education for sustainable living in teacher education. First are those that can be ascribed to the formal education system that, while clinging to Western, Eurocentric values on the one hand, bave also failed to secure a policy for environmental education on the other. Second are the barriers ascribed to the teacher educators themselves, with the whole notion of their powerlessness at its core. Finally, there are the logistical barriers, which encompass, for example, time and financial constraints. With these barriers as a backdrop, to facilitate the incorporation of environmental education into initial teacher education, the study identifies a need for the development of a strategy to secure that education for sustainable living assumes its rightful place in the curriculum for initial teacher education. This framework emerges from the theory grounded in the interviewees' responses during the research, and from the theory grounded in the literature. Central to this framework is for education for sustainable living to contribute to the realisation of real change, change that would further the transformation of our conflict-riddled and inequitable society towards a more democratic and just one. This thesis demonstrates that the realisation of the changes necessary for education for sustainable living demand a reconstruction of current teacher education in order to secure and to sustain an appropriate and sound education ethic to form the basis of a trans formative teacher education curriculum for sustainable living within initial teacher education. Except formal policy, but central to overcoming these barriers, is the need for professional development programmes for teacher educators. A strategy in this regard, is outlined.
- Full Text:
- Date Issued: 1997
Ecological interaction between the introduced and native rock-dwelling cichlid fishes of Lake Malawi National Park, Malawi
- Authors: Munthali, Simon Muchina
- Date: 1997
- Subjects: Cichlids -- Nyasa, Lake
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5266 , http://hdl.handle.net/10962/d1005110
- Description: More than twenty years ago, over twenty species of the rock-dwelling cichlid species (Mbuna) were translocated from the northern Lake Malawi, where they are endemic, to Thumbi West Island, Lake Malawi National Park, in the southern part of Lake Malawi. Among these species, Cynotilapia afra, Pseudotropheus callainos and Pseudotropheus tropheops 'red cheek' are strongly territorial, and have increased substantially in number and are widely distributed, particularly in the three to seven metre depth band of the rocky habitats at the Island of Thumbi West. It is feared that the increase in population density of translocated species (hereafter referred to as introduced species) may be at the expense of ecologically equivalent native species which could be eliminated. In this thesis the following key hypotheses have been tested: (i) that the introduced species having originated from a region of Lake Malawi which is generally poor in nutrients and introduced in an area which is richer in nutrients, would cope better than the native species during periods of nutrient scarcity which occur frequently, often seasonally in oligotrophic lakes, such as Lake Malawi; (ii) that the introduced species are fitter than their ecologically equivalent native species in the acquisition of territorial space in which they breed, feed and seek shelter, and (iii) that introduced and native species coexist by utilizing different microhabitats. Results show that: 1. the introduced species, P. callainos and P. tropheops 'red cheek' may have responded positively to enhanced nutrient availability, as they were found to have better condition factors and fecundity indices at Thumbi West Island than at sites of their origin, in the northern lake Malawi. Cynotilapia afra, P. callainos and P. tropheops 'red cheek' also maximise their life-span fecundity by starting to reproduce at relatively smaller size than the native species with which they overlap in microhabitat requirements. Similarly, their breeding peaks precede the breeding peaks of the native species with which they overlap in microhabitat requirements. Consequently, due to priority residence effects, the offspring of introduced species may have a competitive edge in the use of essential resources, e.g., refuge over the offspring of the native species whose peak-recruitment occurs later in the year. 2. There is an overlap between the introduced and native species in their microhabitat requirements. Consequently, interference competition between them for territorial sites occurs. The choice of optimal territory sites is constrained by the fact that females preferentially mate with males that defend significantly smaller holes, or crevices among the rocks, probably as a means of minimizing egg predation during spawning. 3. The population of territorial males of introduced species seems to grow exponentially, depending on the availability of suitable microhabitats, and an equilibrium between them and males of the native species may be reached. Competition for optimal territory sites seems to intensify, once the carrying capacity in a particular area has been reached, and it is at this stage that some territorial males of the introduced and native species with similar microhabitat requirements, e.g., C. afra and P. zebra, or P. tropheops 'red cheek' and its sibling native species, P. tropheops 'orange chest' displace each other. However, it seems unlikely that any of the native species which were compared with the introduced species would be driven to extinction because: (a) there is a considerable interspecific territory turn-over between the introduced and native species that overlap in microhabitat requirements. (b) Even in situations where some of the native species occur in microhabitats that are not of their preference, they occupy patches of suitable sites and are capable of breeding. (c) It has been suggested that since introduced and native species breed throughout the year and are polygamous and have intraspecifically shared paternity, they are capable of fertilizing many gravid females of their own species. Therefore, the population of native species may not be detrimentally limited by the presence of introduced species. (d) The introduced and native Mbuna species that prefer small rocks coexist in the same microhabitats, partly by feeding at different sites with different intensity and they also feed at different heights in the water column. 4. The following studies have been recommended before any management intervention, such as culling is adopted: (i). interaction between the introduced and native species in the shallow and deep rocky habitats; (ii) space utilization and survivorship of juveniles of the introduced and native species; (iii) laboratory studies to confirm the role of different nutrient regimes on the fecundity of Mbuna; (iv) the possibility of hybridization between the introduced and native species; (v) monitoring of population growth and distribution of the introduced species around Thumbi West Island should continue in order to detect their long-term effects on the native species.
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- Date Issued: 1997