Extraction, Isolation and Biological studies of Pentanisia prunelloides and Hippobromus pauciflorus
- Authors: Ngcetane , Lazola Luyolo
- Date: 2020
- Subjects: Medicinal plants Antioxidants
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: http://hdl.handle.net/10353/18766 , vital:42731
- Description: Enormous amounts of drugs or drug components have been found with the use and help of ethnobotanical knowledge of plants by the indigenous civilisation of that region. The primary reason that plants are chosen as a potential source of new medicines is that they are readily available to researcher and the public either free of charge or at a low and affordable cost in the rural majority community in the developing world. The aim of this study was to evaluate the chemical content of two commonly used medicinal plants of the Eastern Cape Province of South Africa and to explore their antioxidant potential. Crude extracts of Hippobromus pauciflorus and Pentanisia prunelloides were obtained by means of sequential solvent extraction followed by evaluation of the phenolic and flavonoid content of extracts and the anti-oxidant ability of the both plant samples and also their ability to hinder lipid peroxidation. For the H.pauciflorus sample, different masses of crude extracts were obtained with Dichloromethane (0.817 g) yielding the smallest mass and the 70% Ethanol (120.196 g) extract yielding the largest mass. The total phenolic content of the plant sample extracts were measured using the Folin-Ciocalteau reagent. The Methanol extract (133.019 ± 0.003 μgGAE/mL) exhibited the largest content while the EtOH extract (13.981 ± 0.001 μgGAE/mL) exhibited the least content. The MeOH extract exhibited the highest flavonoid content (46.005 ± 0.001 μgQE/mg extract) while the Hexane extract (19.000 ± 0.008 μgQE/mg extract) exhibited the least. 4 Final Submission of Thesis, Dissertation or Research Report/Project, Conference or Exam Paper Frap assay was performed with the Ethyl Acetate extract displaying the least anti-oxidant ability (10.284 ± 0.014 AAE/mL) while the Hex extract (204.705 ± 0.119 AAE/mL) displayed the highest anti-oxidant ability. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay was performed with the hexane, methanol and ethanol extracts exhibiting an anti-oxidant property with IC50 values of 3.594x10-3 ± 0.133 mg/mL, 0.185 ± 0.019 mg/mL and 0.011 ± 0.002 mg/mL respectively. Inhibition of lipid peroxidation at 150 μg/mL was performed where the Dichloromethane extract showed the highest inhibition percentage (-75.000 ± 0.119%) while the Hex extract (-41.667 ± 0.021005%) showed the lowest inhibition percentage at 300 μg/mL; only the DCM extract (-3.051 ± 0.018%) displayed inhibition ability. For the P.prunelloides sample, different masses of crude extracts were obtained with EA extract (0.348 g) yielding the smallest mass and the MeOH (47.941 g) extract yielding the largest mass. Phenolic content was evaluated with the DCM extract (122.827 ± 0.010 μgGAE/mL) exhibited the largest content while the MeOH extract (48.788 ± 0.001 μgGAE/mL) exhibited the least content. Flavonoid content was evaluated with the DCM extract (88.543 ± 0.005 μgQE/mg extract) exhibited the largest content while the EtOH extract (19.254 ± 0.001 μgQE/mg extract) exhibited the least content. FRAP assay was performed with the DCM extract (13.021 ± 0.008 AAE/mL) displaying the least anti-oxidant ability while the MeOH extract (217.758 ± 0.025 AAE/mL) displaying the highest anti-oxidant ability. DPPH assay was conducted with EA and methanol extracts exhibiting antianti-oxidant ability and having IC50 values of 0.579 ± 0.021 mg/mL and 0.006± 0.001 mg/mL respectively. Inhibition of lipid peroxidation at 150 μg/mL was performed with the n-Hex extract (-36.395 ± 0.015%) showing the highest inhibition percentage while the DCM extract (-17.647 ± 0.005%) the lowest and at 300μg/mL the EA (- 12.881 ± 0.018%) and DCM (-0.847 ± 0.009%) extracts displayed inhibition. An attempt was made to isolate and elucidate the compounds in the extracts but unsuccessful, although the TLC results indicated several compounds that can be elucidated in future study
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- Date Issued: 2020
The effectiveness of HIV/AIDS programmes in tertiary institutions of higher learning: the case of the University of Forte Hare
- Authors: Chiwara, Emmanuel
- Date: 2020
- Subjects: HIV infections AIDS (Disease)
- Language: English
- Type: Thesis , Masters , MSW
- Identifier: http://hdl.handle.net/10353/18085 , vital:42228
- Description: This study explored the effectiveness of HIV/AIDS programmes in tertiary institutions in South Africa, and used the University of Forte Hare as a research domain. The study entailed making a cross-examination of the UFH HIV/AIDS programmes in an attempt to test the effectiveness of HIV/AIDS programmes in South African Universities. The study had three objectives, which were to establish the perceptions of the UFH stakeholders pertaining HIV/AIDS programmes; to document the HIV/AIDS programmes offered at UFH; and to determine the resources available to implement the HIV/AIDS Programmes at UFH. The study used mixed methods, that is, incorporating both quantitative and qualitative approaches. The study used combined methods of data collection and instruments, such as in-depth interviews, focus groups interviews, key informants, and a questionnaire. The study adopted Structural Functionalist Theory. The researcher drew a sample of forty (40) participants from the UFH community. Thirty-three (33) student respondents were provided with questionnaire interview; while 5 (five) members of the staff; two (2) members of the UFH HIV/AIDS managers; and two (2) members of administration were interviewed. The study used a combination of both probability and non-probability sampling methods, specifically purposive sampling and systematic sampling methods to select the participants. The data was analyzed using a qualitative and quantitative approaches. The study established that there was inadequacy of financial and material resources to support the implementation of the programmes; inconsistency in condom distribution on campus; limited and unprofessional HIV/AID workers at the UFH campus; availability of various HIV/AIDS programme activities; adequate support structures but failing to be responsive towards HIV/AIDS; and adequate HIV/AIDS knowledge among the students.
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- Date Issued: 2020