A stability-indicating high performance liquid chromatographic assay for the determination of orlistat in capsules
- Authors: Mohammadi, Ali , Haririan, I , Rezanour, I , Ghiasi, L , Walker, Roderick B
- Date: 2006
- Language: English
- Type: Article , text
- Identifier: vital:6407 , http://hdl.handle.net/10962/d1006480
- Description: A stability-indicating HPLC method was developed and validated for the quantitative determination of orlistat in capsule dosage forms. An isocratic separation was achieved using a Perfectsil® target ODS-3, 250 mm × 4.6 mm i.d., 5 µm particle size column with a flow rate of 0.7 ml/min and using a UV detector to monitor the eluate at 210 nm. The mobile phase consisted of methanol:acetonitrile:trifluoroacetic acid (82.5:17.5:0.01, v/v/v). The drug was subjected oxidation, hydrolysis, photolysis and heat to apply stress conditions. Complete separation was achieved for the parent compound and all degradation products in an overall analytical run time of approximately 15 min with the parent compound orlistat eluting at approximately 9 min. The method was linear over the concentration range of 0.02–0.75 mg/ml (r = 0.9998) with a limit of detection and quantitation 0.006 and 0.02 mg/ml, respectively. The method has the requisite accuracy, selectivity, sensitivity and precision to assay orlistat in capsules. Degradation products resulting from the stress studies did not interfere with the detection of orlistat and the assay is thus stability-indicating.
- Full Text:
- Date Issued: 2006
Academic Library Consortia in South Africa : where we come from and where we are heading
- Authors: Thomas, G M E , Fourie, I
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:6987 , http://hdl.handle.net/10962/d1012410
- Description: The purpose of this article is to give an overview of the establishment of the five academic library consortia in South Africa, their status quo, and a summary of their successes and plans for the future. Although useful information can be found from the consortia Web sites, much of it is no longer current. Similarly, there is limited published literature on the academic library consortia in South Africa. Apart from the sources referenced, the authors therefore relied heavily on an unpublished conference paper by Thomas[reference 1], which included findings gathered during a short survey among the five academic library consortia. In addition, Rowley and Slack[reference 2] and Sekabembe[reference 3] provide useful overviews of the library consortia in South Africa at the time of publication. Further information is available in published and unpublished sources.
- Full Text:
- Date Issued: 2006
Application of reverse-flow micellar electrokinetic chromatography for the simultaneous determination of flavonols and terpene trilactones in Ginkgo biloba dosage forms
- Authors: Dubber, Mary-Jean , Kanfer, Isadore
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:6357 , http://hdl.handle.net/10962/d1006049
- Description:
A reverse-flow micellar electrokinetic chromatographic (RF-MEKC) method was developed for the simultaneous qualitative determination of 10 components consisting of the flavonol glycosides, rutin and quercitrin, the flavonol aglycones, isorhamnetin, kaempferol and quercetin, the terpene trilactones, ginkgolides A, B, C and J and the sesquiterpene, bilobalide. This method was used to fingerprint Ginkgo biloba solid oral dosage forms and validated for the quantitation of the marker compounds, rutin and quercetin in some commercial products. In addition to the usual variables, the influence of some essential background electrolyte (BGE) components such as sodium dodecyl sulphate (SDS) and
-cyclodextrin concentrations were investigated. A polyimide fused-silica square capillary column (75 μm I.D. × 360 μm O.D.) with a total length of 60.0 cm and effective length of 45.0 cm was used for the separation. The final BGE consisted of 20 mM phosphoric acid, 40 mM SDS and 12 mM -cyclodextrin (pH 2.2) using reverse polarity with a voltage of −17.5 kV. Samples were injected electrokinetically at −5 kV for 3 s for the qualitative analysis and hydrodynamically at 20 mbar for 0.6 s for the quantitative assay. The total run time was 22 min and the limits of detection were 3.13 μg/ml and 1.88 μg/ml for rutin and quercetin, respectively. Fingerprint profiles of the solid oral dosage forms and the results of the quantitative analysis indicated that there were major discrepancies in the marker content between products and illustrates the value of this method for use as a procedure to assess product quality of commercially available Ginkgo biloba products. - Full Text:
- Date Issued: 2006
Comprehension and acceptability of a patient information leaflet (PIL) for antiretroviral therapy:
- Authors: Mwingira, Betty , Dowse, Roslind
- Date: 2006
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/156902 , vital:40065 , https://doi.org/10.4102/hsag.v11i3.236
- Description: The patient information leaflet (PIL) is recognised as playing a key role in informing patients about their medicines. The objectives of this research were to evaluate the readability and understanding of a PIL for the first-line ARV (antiretroviral) regimen available in the South African public health sector, and investigate its acceptability in the target Xhosa population. The study took place between August 2003 and July 2004. A PIL was designed for the antiretroviral regimen of stavudine, lamivudine and efavirenz, using established usability guidelines. South African legal requirements concerning PILs were incorporated and the PILs were available in both English and isiXhosa.
- Full Text:
- Date Issued: 2006
Effects of ring substituents on electrocatalytic activity of manganese phthalocyanines towards the reduction of molecular oxygen
- Authors: Sehlotho, Nthapo , Nyokong, Tebello
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:6594 , http://hdl.handle.net/10962/d1004343
- Description: Reduction of oxygen electrocatalyzed by adsorbed films of manganese phthalocyanine complexes is reported. The complexes studied were: manganese phthalocyanine (MnPc, 1); manganese tetraamino phthalocyanine (MnTAPc, 2); manganese tetrapentoxy pyrrole phthalocyanine (MnTPePyrPc, 3); manganese tetra phenoxy pyrrole phthalocyanine (MnTPPyrPc, 4); manganese tetra mercaptopyrimidine phthalocyanine (MnTMPyPc, 5) and manganese tetra ethoxy thiophene phthalocyanine (MnTETPc, 6). The reaction was conducted in buffer solutions of pH range 1–12. Rotating disk electrode voltammetry revealed two electron reduction in acidic and slightly alkaline media due to the formation of hydrogen peroxide. In highly basic media, water is the major product formed via four electron transfer. The reaction was found to be first order in the diffusing analyte oxygen.
- Full Text:
- Date Issued: 2006
Enabling environmental and sustainability education in South Africa’s national curriculum: Context, culture and learner aspirations for agency
- Authors: Lotz-Sisitka, Heila
- Date: 2006
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/437486 , vital:73388 , ISBN 978-1594549458
- Description: This chapter describes the broad agenda for cultural and social change in South Africa, as introduced by post-apartheid policy development. It briefly describes how environment and sustainability questions have been incorporated into the National Curriculum Statements, alongside a concern for culture, context and indigenous knowledges. The chapter considers some of the contemporary concerns with the disjuncture be-tween policy and practice, and highlights learner aspirations for agency, and associated expectations for education. The chapter draws its orien-tation from the Rhodes University Environmental Education and Sus-tainability's' Environmental Learning and Curriculum' research pro-gramme. This programme is funded by the National Research Founda-tion and aims to inform curriculum change in South Africa. It programme incorporates a strand on indigenous knowledge and curriculum.
- Full Text:
- Date Issued: 2006
Estimation of small reservoir storage capacities in Limpopo River Basin using geographical information systems (GIS) and remotely sensed surface areas: case of Mzingwane catchment
- Authors: Sawunyama, Tendai , Senzanje, J , Mhizha, A
- Date: 2006
- Language: English
- Type: Article
- Identifier: vital:7077 , http://hdl.handle.net/10962/d1009741
- Description: The current interest in small reservoirs stems mainly from their utilization for domestic use, livestock watering, fishing and irrigation. Rarely were small reservoirs considered in the water resources system even though they are important in water resource planning and management. The main limitation being lack of knowledge on small reservoir capacities, for the methodologies used to quantify physical parameters of reservoirs are costly, time consuming and laborious. To address this challenge an attempt has been made in this study to estimate small reservoir storage capacities using remotely sensed surface areas. A field study on 12 small reservoirs was carried out in Mzingwane catchment in Limpopo River Basin; Zimbabwe. The depths of water accompanied with their coordinates were measured; from which area and capacity were calculated for each reservoir using geographical information system based on data acquired from the field and that from satellite images. The output data was compared and a linear regression analysis was carried out to establish a power relationship between surface area and storage capacity of small reservoirs. The Pearson correlation analysis at 95% confidence interval indicated that the variances of the two surface areas (field area and image area) were not significantly different (p < 0.05). The findings from linear regression analysis (log capacity–log area) show that there exist a power relationship between remotely sensed surface areas (m^2) and storage capacities of reservoirs (m^3), with 95% variation of the storage capacity being explained by surface areas. The relationship can be used as a tool in decision-making processes in integrated water resources planning and management in the river basin. The applicability of the relationship to other catchments requires further research as well as investigating the impacts of small reservoirs in water resources available in the river basin by carrying out a hydrological modelling of the catchment.
- Full Text:
- Date Issued: 2006
Lattice-valued continuous convergence is induced by a lattice-valued uniform convergence structure
- Authors: Jäger, Gunter
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:6824 , http://hdl.handle.net/10962/d1012332
- Description: We define a stratified L-uniform convergence structure on the set of all continuous mappings from a stratified L-limit space to a stratified L-uniform convergence space. This structure induces L-continuous convergence. This shows that the category of all L-limit-uniformizable spaces is cartesian closed.
- Full Text:
- Date Issued: 2006
Olive Schreiner at 150: some thoughts on re-editing Cronwright's The Reinterment on Buffelskop
- Authors: Walters, Paul S , Fogg, W Jeremy M
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:6124 , http://hdl.handle.net/10962/d1004708
- Description: [From the introduction]: The original edition of Cronwright’s The Reinterment on Buffelskop (1983) was produced by Guy Butler and Nick Visser to commemorate the centenary of the 1883 publication of The Story of an African Farm. The Butler-Visser text was a photographic reproduction of a typed carbon copy of the first part of Cronwright’s extant diaries plus a special diary he had kept covering in detail the events of the actual reinterment. (The originals are now at the National English Literary Museum [NELM].) Butler included a comprehensive and illuminating introduction to these texts, as well as – under separate soft cover – a set of “Provisional Notes” which draw deeply on his own and his family’s accumulated knowledge of Cradock, its environs and inhabitants. In addition, Butler and Visser included two passages excised by Cronwright from the typescript of his Life of Olive Schreiner: a word picture of Charles Heathcote, and the longer account of “The Nienaber Incident” – pages which deal with the execution of three innocent men at De Aar on 19 March 1901, and Cronwright’s subsequent attempts at legal reparation for them and their families. The substantive text of the Butler-Visser edition is often difficult to read because of the method of reproduction; moreover, because it also reproduces Cronwright’s emendations (in ink) of the typescript, it is frankly uninviting. Thus, when the NELM Council proposed a publication commemorating the 150th anniversary of Olive Schreiner’s birth on 24 March 1855, it seemed appropriate that a second attempt be made to give students of Olive Schreiner’s works easier access to Cronwright’s detailed account of this “bizarre, romantic” episode. Furthermore, from the perspective of text history, the typescript of the Reinterment antedates both Cronwright’s Life and The Letters of Olive Schreiner. Parts of it are clearly Cronwright’s preliminary ‘notes towards’ his Life, and, as Butler hypothesizes, the whole of the Reinterment might have been intended as a separate (and earlier) publication. Finally, the sarcophagus on Buffelskop is one of South Africa’s more noteworthy literary shrines: while the idea of re-editing an account of Olive Schreiner’s reinterment might be thought to be a futile exercise in intellectual recycling, our intention is that both husband and wife should live again through a rediscovery of the thoughts and feelings that led them to this dramatic final resting-place.
- Full Text:
- Date Issued: 2006
Predictor variables for moggel (Labeo umbratus) biomass and production in small South African reservoirs
- Authors: Potts, Warren M , Booth, Anthony J , Hecht, Thomas , Andrew, Timothy G
- Date: 2006
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125763 , vital:35815 , https://doi.10.2989/16085910609503877
- Description: South Africa has approximately 3 100 registered reservoirs, ranging in size from 1–1 000 hectares, with a surface area totalling 84 439 hectares (SADC Surface Water Body Database, unpublished data). Within southern and eastern Africa, Lindqvist (1994) estimated the number of small reservoirs to be between 50 000 and 100 000. Given Bernacsek’s (1986) estimate of the total fishery potential of small reservoirs in Africa at between 1 and 2.3 million tons, this number of reservoirs clearly could provide fishery opportunities for rural communities.
- Full Text:
- Date Issued: 2006
Spatial description of hake-directed fishing activity off the west coast of South Africa
- Authors: Fairweather, T P , Booth, Anthony J , Sauer, Warwick H H , Leslie, Rob W
- Date: 2010
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/127031 , vital:35945 , https://doi.10.2989/18142320609504129
- Description: Historically, the two species of Cape hakes Merluccius capensis and M. paradoxus off South Africa were commercially exploited exclusively by demersal trawling. In 1994, hake-directed demersal longline was introduced on an experimental basis, and in 1998 was initiated as a commercial fishing sector. The effect of a combined fleet composed of both trawlers and longliners on the Cape hake resource is not fully understood. Analysis of fishing intensity and catch-rate data revealed that the highest catch rates were found around the 400m and 500m isobaths for the trawl and longline fisheries respectively. Catch rates from both fishing sectors were also noted to be higher over sediments with a sand component. Differences between areas of the highest fishing intensity and highest catch rates were noted. In addition to other factors, it is suggested that a ‘friction of distance’ effect applies — vessels will trade-off higher catch rates with the increased costs associated with fishing in deeper waters.
- Full Text:
- Date Issued: 2010
The importance of phytoplankton size in mediating trophic interactions within the plankton of a southern African estuary
- Authors: Froneman, P William
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:6928 , http://hdl.handle.net/10962/d1011944
- Description: The influence of the phytoplankton size composition in mediating the trophic interactions between the bacteria, phytoplankton, microheterotrophs (<200 μm) and mesozooplankton (>200 μm) was investigated on three occasions in a warm temperate, temporarily open/closed estuary situated along the southern African coastline. Results of the investigation indicated that the microheterotrophs represented the most important consumers of bacteria and chlorophyll (chl)-a <5.0 μm. The low impact of the mesozooplankton on the bacteria and chl-a <5.0 μm during the study appeared to be related to the inability of the larger zooplankton to feed efficiently on small particles. During those periods when total chl-a concentration was dominated by picophytoplankton (<2.0 μm) and microphytoplankton (>20 μm), mesozooplankton were unable to feed efficiently on the chl-a due to feeding constraints. In response to the unfavorable size structure of the phytoplankton assemblages, mesozooplankton appeared to consume the microheterotrophs. The negative impact of the mesozooplankton on the microheterotrophs resulted in a decrease in the impact of these organisms on the bacteria and the chl-a <5.0 μm. This result is consistent with the predator-prey cascades. On the other hand, when the total chl-a was dominated by nanophytoplankton (2–20 μm), mesozooplankton were able to feed directly on the phytoplankton. Results of the study indicate that size structure of the phytoplankton assemblages within estuaries plays an important role in mediating the trophic interactions between the various components of the plankton food web.
- Full Text:
- Date Issued: 2006