A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories
- Authors: Mohammadi, I , Kanfer, Isadore , Walker, Roderick B
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6406 , http://hdl.handle.net/10962/d1006479
- Description: Current compendial methods of assay for the analysis of cyclizine tablets involve the use of UV spectrophotometry. Since this is a non-selective technique its application to more complex dosage forms, such as suppositories, is unlikely to be appropriate. There is therefore a need for the development of a highly specific quantitative analytical method, such as high performance liquid chromatography (HPLC) or capillary electrophoresis (CE). The latter technique was chosen in view of some specific advantages over HPLC, such as the use of relatively non-toxic aqueous buffers, as opposed to organic solvents, which obviates the use of expensive HPLC grade solvents making CE more cost effective. Cyclizine was analyzed in 50 mM phosphate buffer (pH 2.3) and run at an applied voltage 25 kV. Detection sensitivity was enhanced by using a wavelength of 200 nm and samples were loaded hydrodynamically onto an uncoated fused-silica capillary (60 cm×50 mm i.d.). Chlorcyclizine was used as the internal standard and resolution of both compounds was achieved in less than 7 min. Stress testing was undertaken in order to investigate the appearance of breakdown products. The method has the requisite accuracy, selectivity, sensitivity and precision to assay cyclizine in tablets and suppositories. Degradation products resulting from the stress studies did not interfere with the detection of cyclizine and the assay is thus stability-indicating.
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- Date Issued: 2004
A Trypanosoma cruzi heat shock protein 40 is able to stimulate the adenosine triphosphate hydrolysis activity of heat shock protein 70 and can substitute for a yeast heat shock protein 40
- Authors: Edkins, Adrienne L , Ludewig, M H , Blatch, Gregory L
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6465 , http://hdl.handle.net/10962/d1005794 , http://dx.doi.org/10.1016/j.biocel.2004.01.016
- Description: The process of assisted protein folding, characteristic of members of the heat shock protein 70 (Hsp70) and heat shock protein 40 (Hsp40) molecular chaperone families, is important for maintaining the structural integrity of cellular protein machinery under normal and stressful conditions. Hsp70 and Hsp40 cooperate to bind non-native protein conformations in a process of adenosine triphosphate (ATP)-regulated assisted protein folding. We have analysed the molecular chaperone activity of the cytoplasmic inducible Hsp70 from Trypanosoma cruzi (TcHsp70) and its interactions with its potential partner Hsp40s (T. cruzi DnaJ protein 1 [Tcj1] and T. cruzi DnaJ protein 2 [Tcj2]). Histidine-tagged TcHsp70 (His-TcHsp70), Tcj1 (Tcj1-His) and Tcj2 (His-Tcj2) were over-produced in Escherichia coli and purified by nickel affinity chromatography. The in vitro basal specific ATP hydrolysis activity (ATPase activity) of His-TcHsp70 was determined as 40 nmol phosphate/min/mg protein, significantly higher than that reported for other Hsp70s. The basal specific ATPase activity was stimulated to a maximal level of 60 nmol phosphate/min/mg protein in the presence of His-Tcj2 and a model substrate, reduced carboxymethylated α-lactalbumin. In vivo complementation assays showed that Tcj2 was able to overcome the temperature sensitivity of the ydj1 mutant Saccharomyces cerevisiae strain JJ160, suggesting that Tcj2 may be functionally equivalent to the yeast Hsp40 homologue (yeast DnaJ protein 1, Ydj1). These data suggest that Tcj2 is involved in cytoprotection in a similar fashion to Ydj1, and that TcHsp70 and Tcj2 may interact in a nucleotide-regulated process of chaperone-assisted protein folding.
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- Date Issued: 2004
Sex work from a feminist perspective: a visit to the Jordan case
- Authors: Krüger, Rósaan
- Date: 2004
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68877 , vital:29335 , https://doi.org/10.1080/19962126.2004.11864812
- Description: Publisher version , Introduction: Contributors to the prostitution/sex work debate, whether they condone, support or oppose criminalisation, legalisation or decriminalisation of prostitution, often rely on ‘facts’ to support their arguments. A common fact is that the majority of prostitutes/sex workers in the world and in South Africa are women. Thus, when I refer to prostitutes/sex workers, I refer to women working as prostitutes in the commercial sex industry. Furthermore, the fact that the majority of sex workers are women justifies considering prostitution from a feminist perspective – women’s voices on the subject should be heard. In this note I shall use the terms ‘prostitution’ and ‘sex work’ to refer to the exchange of sexual services for money. The former, more conventional term has a negative connotation, while the term ‘sex work’ denotes a movement away from casting a moral judgment towards recognition that sex work is just another job. References will be made to the associated activities of brothel-keeping and pimping, but the focus of this note is mainly on the provision of sexual services by the prostitute/ sex worker herself. Jordan v S is a Constitutional Court judgment in which the constitutional validity of the criminalisation of prostitution and its related activities were challenged. In order to analyse this judgment from a feminist perspective, I shall first briefly set out the current legal position on prostitution. Thereafter, I shall give an overview of Western feminist perspectives on prostitution and then link this perspective with African feminism. The last part of the note will be an analysis of the Jordan judgment in light of the feminist perspectives identified before.
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- Date Issued: 2004
Transforming the media: a cultural approach
- Authors: Steenveld, Lynette N
- Date: 2007
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147891 , vital:38682 , DOI: 10.1080/02560240485310061
- Description: The change from an Apartheid state to a liberal democratic one has wrought many changes at all levels of South African society: the economic, social, political, cultural. This paper explores the impacts of these changes on the South African print media industry, with a view to assessing their contribution to the development of a democratic citizenship. While acknowledging the constraining effects of economic structures of ownership, the paper locates these within the broader social and political context of post-apartheid South Africa. It thus attempts to synthesise elements of both a political economy and cultural approach to the analysis of cultural production.
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- Date Issued: 2007