The priesthood of all believers : a re-statement
- Authors: Borman, John
- Date: 1966
- Subjects: Priesthood, Universal , Christian life , Church renewal , Ministry and Christian union
- Language: English
- Type: Thesis , Bachelor , BDiv
- Identifier: vital:1286 , http://hdl.handle.net/10962/d1013515
- Description: From Preface: In this age of ecumenical discussion it has becone necessary for all Christian communions to examine afresh their traditional beliefs. This has frequently led to restatement and reformulation of such traditional beliefs. It has also driven us back to seek new light from the Biblical sources. It seemed that such a re-examination of the traditional doctrine of the priesthood of all believers was necessary so that those who claim to hold it know what they hold, and so that those who do not may know what it is that their fellow-Christians believe, and why. This was the first reason for this study.
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- Date Issued: 1966
A contribution to the biology of warthog (Phacochoerus africanus, Gmelin) in the Sengwa region of Rhodesia
- Authors: Cumming, D H M
- Date: 1971
- Subjects: Warthog Warthog -- Physiology Warthog -- Ecology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5843 , http://hdl.handle.net/10962/d1010740
- Description: To the AmaZulu a warthog is "inhlovudawana" or "little elephant"; to arrogant hunters it is the "poor man's rhinoceros"; to some writers of encyclopaediae it is a "grotesque and hideous beast"; to stockmen it has long been a carrier of diseases. Injury was added to insult with the discovery that the blood of these self-assured, often comical and certainly engaging animals supports the greater proportion of tsetse flies in the African savannas. Their significance as the primary hosts of Glossina morsitans Westw., the vectors of tryanosomiasis, justified an extended field study of warthog biology. This thesis reports four years of field work on warthogs, together with complementary observations of hand-reared warthogs (and their offspring) which roamed freely in the vicinity of the remote field station on which I live. The Sengwa Research Project, of which this study forms a part, was initiated in 1965 to study relationships between game animals and tsetse flies. One of the main problems investigated in the Sengwa Project is that of "host encounter" (Glasgow, 1961, Bursell, 1970) and how the distribution, abundance and behaviour of game animals may affect their availability to hungry tsetse flies. I have, accordingly, been concerned with discovering how warthog are dispersed in the Sengwa area and have attempted to gain some understanding of the factors, both environmental and social, which may affect or govern their dispersion and possibly population number. Intro. p. 1.
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- Date Issued: 1971
The keyboard sonatas of Pietro Augusto : an investigation into their formal and stylistic apsects
- Authors: Radloff, Timothy Edward Kingswell
- Date: 1976
- Subjects: Augusto, Pietro , Sonatas (Piano)
- Language: English
- Type: Thesis , Masters , MMus
- Identifier: vital:2689 , http://hdl.handle.net/10962/d1013047
- Description: This thesis supplements "The Piano Sonata of the Eighteenth Century in Germany" (Vol. 2 in the publication series "Contributions to the development of the Piano Sonata"). It does not aim at a comparative study between Pieto Augusto and his contemporaries but intends to establish the composer's individual characteristics in the light of the general development of the piano sonata during the eighteenth century.
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- Date Issued: 1976
The in-service development of primary school teachers in the use of computers in education with special reference to computer assisted learning: a case study
- Authors: Murray, John Charles
- Date: 1992
- Subjects: Education, Elementary -- South Africa -- Data processing Computer-assisted instruction Teachers -- In-service training -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1572 , http://hdl.handle.net/10962/d1003454
- Description: Many white primary schools in South Africa have acquired computers which are being used in various ways. In my experience few members of staff are confident enough to use the computers in the school for educational purposes. Those who have been using them, generally have been doing computer literacy. The teachers in our school expressed the view that they would like to be able to use the computer, but that they lack the necessary skills and therefore would welcome some form of training. In my opinion computers should form an integral part of a child's learning environment. It has, for some time formed a part of his social environment, either in the home or in the workplace. For computers to be used effectively in the classroom, it follows that the teachers need to be trained to use them. In an attempt to overcome this problem, I designed and presented an in-service course, which used selected computer assisted learning (CAL) programs with teachers. These teachers then used these programs with the pupils in their class and evaluated the pupils' reactions to the innovation. As a result of the observations and the feedback provided by the sample of teachers, it is suggested that this might be a possible approach to in-service training for other schools. With adaptation it should be possible to follow this approach in any school which has a computer and someone on the staff who is willing to take the initiative to implement such an innovation.
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- Date Issued: 1992
'People's English' in South Africa : theory and practice
- Authors: Butler, Ian
- Date: 1994
- Subjects: English language -- Study and teaching -- South Africa Second language acquisition -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1691 , http://hdl.handle.net/10962/d1003574
- Description: Communicative Language Teaching, an approach to language teaching currently widely prescribed, has been criticised for its unquestioning acceptance of the cultural and political norms of the target language. People's English (an aspect of the larger People's Education movement), on the other hand, offers a philosophy and methodology that takes an actively critical view of language and its relationship to power. In the context of South African society, this stance has had clear political implications. Although still imprecisely defined , the concepts of People's Education and People's English have been debated and discussed by various ant-apartheid movements in recent years. Attempts have also been made to translate the evolving theory into practice through the development of materials and methodologies. This has been achieved on a relatively small scale, with varying degrees of success. Recent reform measures by the South African government have, however, prompted the proponents of People's Education to reassess their position. This thesis presents a historical overview and critical assessment of the development of People's English in South Africa.
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- Date Issued: 1994
Photosensitizing properties of non-transition metal porphyrazines towards the generation of singlet oxygen
- Authors: Seotsanyana-Mokhosi, Itumeleng
- Date: 2001 , 2013-05-02
- Subjects: Phthalocyanines , Photosensitization, Biological , Active oxygen -- Physiological effect , Photosensitizing compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4395 , http://hdl.handle.net/10962/d1006086 , Phthalocyanines , Photosensitization, Biological , Active oxygen -- Physiological effect , Photosensitizing compounds
- Description: Metallophthalocyanine complexes containing non-transition metals are very useful as sensitizers for photodynamic therapy, a cure for cancer that is based on visible light activation of tumour localized photo sensitizers. Excited sensitizers generate singlet oxygen as the main hyperactive species that destroy the tumour. Water soluble sensitizers are sought after for the convenience of delivery into the body. Thus, phthalocyanine (pc), tetrapyridinoporphyrazines (tppa) and tetramethyltetrapyridinoporphyrazines (tmtppa) with non-transition central metal atoms of Ge, Si, Sn and Zn were studied. First was the synthesis of these complexes, followed by their characterisation. The characterisation involved the use of ultraviolet and visible absorption spectroscopy, infrared spectroscopy, nuclear magnetic resonance spectroscopy, electrochemical properties and elemental analysis. Photochemical properties of the complexes were then investigated. Photolysis of these macrocycles showed two processes; -reduction of the dye and photobleaching, which leads to the disintegration of the conjugated chromophore structure of the dye. Photobleaching is the reductive quenching of the excited state of the sensitizers. The intensity of the quenching decreased progressively from tmtppa, tppa to pc metal complexes with photobleaching quantum yields, 6.6 x 10.5⁻¹, 1.8 x 10.5⁻¹ and 5.4 x 10⁻⁶ for Zntmtppa, Zntppa and Znpc, respectively. Efficiency of singlet oxygen sensitization is solvent dependent with very different values obtained for the same compound in different solvents, for example, 0.25 and 0.38 were observed as singlet oxygen quantum yields for Gepc complex in DMSO and DMF respectively. In DMSO the efficiency of ¹O₂ generation decrease considerably from pc to tppa and finally tmtppa. In water Getmtppa exhibits much higher singlet oxygen quantum yield, hence promising to be effective as a sensitizer for photodynamic therapy.
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- Date Issued: 2001
Exploring the perceived value of work as part of psychosocial rehabilitation of the state patient : Komani Hospital, Queenstown
- Authors: Crocker, Ann Petro
- Date: 2002
- Subjects: Work -- Psychological aspects Mentally ill -- Attitudes
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:5953 , http://hdl.handle.net/10962/d1007464
- Description: During the research an attempt was made to gain an understanding of the perceived value of work in psychosocial rehabilitation with the Sate patient at Komani Hospital. The respondents' motivation to work and what they gain from working, was investigated, as well as the experience of the nonworking respondents and their motivation not to work. To achieve this, ten respondents who were working and ten respondents who were not working, were interviewed. This study involves only a limited number of State patients at Komani Hospital and therefore findings cannot be generalised and also because the study was done at one Hospital only. A literature study was undertaken during which it was evident that work as part of vocational rehabilitation contributed to the well being of the mentally disabled person. It was also stated in the literature that the disabled person must be actively involved in his/her own rehabilitation planning and that the person must make conscious decisions to move away from the "sick" role and participate in his/her rehabilitation process. Interview schedules were developed. Response received from the twenty participants was analysed. The overall findings of the study showed that the majority of respondents who were working, were motivated by money. The fact that the respondents work from Hospital and therefore disrupt the Hospital routine, work with abled co-workers and feeling needed by the factory where they are employed. also emphasised the respondents' motivation to keep on working. The findings of the study with the non-working group showed that some respondents want to work, but there are not enough work opportunities available in and from Hospital. The non-working respondents are not interested in working at the occupational therapy department because the pay patients get for work done at the department is too little, and therefore they reject the work there. Some respondents do not want to work. whether work is available or not. The non-working group is largely dependent on staff and co-patients for an income, or tobacco. The predominant activity of the non-working group of respondents during the day, as well as that of the majority of the working group respondents during weekends was sleeping, talking to friends, and smoking. The research findings, conclusions and recommendations could contribute to a better understanding of patients and the value of work for the patient in a Psychiatric Hospital.
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- Date Issued: 2002
An investigation of code switching in different learning areas in a grade seven class
- Authors: Ndabeni, Bulelwa
- Date: 2003
- Subjects: Code switching (Linguistics) -- South Africa Language and education -- South Africa Education, Bilingual -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1621 , http://hdl.handle.net/10962/d1003503
- Description: The research described in this thesis is a case study of the communicative repertoires of teachers and learners of a grade seven class in a small rural primary school. The aim of the investigation was to find out if code switching is different in content-subject classes than in an English language class. The study accomplishes this by looking at code switching in different learning areas. Different research techniques such as observations, video. recording, taking of field notes and interviews are used as the means of data collection. The study does not attempt a full linguistic description of the switches made by the research subjects. For example, it does not deal with linguistic constraints on code switching, but instead it focuses on the functional aspects of code switching. The study reveals that there are various functions of code switching in the classroom. The following are the major findings noted in this study: • Code switching is a communicative resource, which enables the teachers and learners to accomplish a considerable number and range of social and educational objectives. • Code switching is used to negotiate and renegotiate meaning. • How teachers code switch in class depends on how difficult the lesson is for the learners. • The study reveals no major differences between code switching In content subjects than in English as a subject. • Although tbe subjects engage in code switching, they seem to feel guilty about their behaviour.
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- Date Issued: 2003
"Feeling foggy?": an investigation into the self-reported post-concussive symptoms in rugby union players at university level
- Authors: Boulind, Melissa
- Date: 2005
- Subjects: Brain damage , Brain -- Wounds and injuries -- Complications , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Rugby football injuries , Rugby Union football players
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2938 , http://hdl.handle.net/10962/d1002447 , Brain damage , Brain -- Wounds and injuries -- Complications , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Rugby football injuries , Rugby Union football players
- Description: A study was conducted on the self-reported symptoms of Mild Traumatic Brain Injury sustained in Rugby Union at the pre- and post-season stages. A full sample of 30 rugby players at Rhodes University was compared to 27 non-contact sport controls. A reduced sample of 20 rugby players and 9 control participants provided improved control for education and IQ and was compared. Measures included the WAIS-III Vocabulary and Picture Completion Sub-tests to estimate IQ level, the symptom checklist on a widely used computer-based program (ImPACT), and a paper and pencil self-report 31-Item Post-Concussion Symptom Questionnaire. Independent and Dependent T-Test comparisons were conducted on the full and reduced samples. The symptoms reported by the rugby group appeared to be more pronounced on both the ImPACT Symptom Scale and the 31-Item Post-Concussion Symptom Questionnaire when compared to the control group at both the pre-and post-season stages. It was concluded that the rugby players demonstrated evidence to support the hypothesis of having sustained more previous concussions and reporting more symptoms at the pre-season stage when compared to comtrol participants. No prevalent changes for either the rugby or control groups were seen in dependent comparisons from pre-to post-season.
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- Date Issued: 2005
Investigating teacher's perceptions about the value and implementation of Arts at the lower primary phase: a case study in selected schools in Namibia
- Authors: Chombo, Stanley Chombo
- Date: 2009
- Subjects: Education -- Namibia Teachers -- Attitudes -- Namibia Arts -- Study and teaching (Elementary) -- Namibia -- Case studies
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1952 , http://hdl.handle.net/10962/d1008207
- Description: This study was conducted in order to investigate teachers' perceptions about the value and implementation of Arts in the Lower Primary Phase. The study adopted a qualitative approach and seeks to investigate (a) teachers' perceptions of the role and value of Arts at the Lower Primary Phase (b) the issues and problems teachers have in teaching Arts as a separate subject at the Lower Primary Phase (c) the opportunities for incorporating Arts with other subjects and with the cross-curricular issues. The data was gathered by using interviews, class observations and document analysis. The study focused on six teachers in two Schools in the Caprivi education region of the Republic of Namibia. The findings of the study revealed that Arts stimulates the learner's imagination and creativity. The teachers felt that by encouraging learners to explore their creativity through Arts we maintain the practice of the Arts through drawing, carving, modelling and music.
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- Date Issued: 2009
The binding roots of free speech
- Authors: Boughey, Thomas John George
- Date: 2009
- Subjects: Freedom of speech
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2849 , http://hdl.handle.net/10962/d1006255
- Description: This thesis argues that the modern notion of free-speech was born within the Westphalian nation-state. It suggests that the legal rights framework - particular to the Westphalian nationstate - not only legitimizes and legalizes the right to free-speech, but also enables us to invoke legally the necessary limitations that demand the limitation of free-speech in certain contexts. However, such a legal-rights framework is exclusive to the nation-state and cannot be enforced on an international level, outside of the nation-state boundary. With reference to examples on an international level, this thesis demonstrates that calls for the limitation of free-speech are indeed legitimate and necessary but cannot be enforced on an international level for the reasons just mentioned. In order to address this problem, this thesis proposes a framework - based on a Kantian model - that enables us to invoke the limitation of free-speech on an international level without appealing to a legal-rights discourse to do so.
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- Date Issued: 2009
Induced plant responses of different Lantana camara L. (Verbenaceae) varieties to herbivory by Falconia intermedia (distant) (Hemiptera: Miridae)
- Authors: Heshula, Unathi-Nkosi Lelethu Peter
- Date: 2010
- Subjects: Lantana camara -- South Africa -- Eastern Cape , Lantana camara -- Biological control -- South Africa -- Eastern Cape , Biological pest control agents -- South Africa -- Eastern Cape , Weeds -- Biological control -- South Africa -- Eastern Cape , Invasive plants -- Biological control -- South Africa -- Eastern Cape , Hemiptera -- South Africa -- Eastern Cape , Miridae -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5911 , http://hdl.handle.net/10962/d1015368
- Description: A highly variable invasive shrub, Lantana camara L. (Verbenaceae), has been notoriously difficult to control thus far despite a well established biological control programme in South Africa. A promising leaf-feeding biological control agent, Falconia intermedia (Distant) (Hemiptera: Miridae), released to control this invasive plant eventually crashed at three out of five sites in the Eastern Cape Province. In the Mpumalanga Province, after initially colonising and building up high numbers on the L. camara stands the agent populations crashed. Several reasons for these population crashes have been suggested, but induced plant defences have not been investigated. Although plants face the challenge of herbivory by various organisms while remaining immobile, some plants may possess the ability to induce physical and/or chemical defensive responses following feeding and thus prevent further plant tissue damage and loss. Laboratory trials were conducted to determine the existence, nature and effect of physical and chemical feeding-induced responses of L. camara on the performance of the leaf-feeding biological control agent, F. intermedia. Lantana camara plants used in the study were obtained from five localities in the Eastern Cape Province, South Africa, while the insect culture was established from field populations. Plants from all varieties on which F. intermedia was released significantly increased the toughness of their leaves compared to control treatment plants. In addition, plants from three localities: Lyndhurst Farm, East London and Port Alfred, significantly increased trichome density after prolonged feeding by F. intermedia. On the three varieties showing increases in these two factors (i.e. leaf toughness and trichome density), oviposition, survival and feeding damage by the mirid agent was significantly lower on previously damaged plants. A significant negative correlation between trichome density and population numbers was found (R²= 0.52, p < 0.0003), suggesting that an increase in trichome density strongly contributes to a reduction in F. intermedia's growth. The growth and reproduction of the resistant plants was not significantly impacted by F. intermedia feeding. The defensive responses were found to be plant systemic and rapidly induced as they were elicited and expressed throughout the plant in both damaged and undamaged leaves within five weeks after insect release. Leaf toughness and trichome density were not significantly increased after feeding on plants from Whitney Farm and Heather Glen Farm. On the contrary, mirid individuals performed significantly better on plants from Whitney Farm and Heather Glen Farm than on plants of other varieties, indicating their susceptibility and suitability to the agent and the lack of induced resistance against the agent. Plants from all localities besides East London showed some level of tolerance and overcompensated for feeding damage by increasing plant growth and reproductive factors on plants fed upon. This was however only significant in two variables of the more susceptible localities, Whitney Farm and Heather Glen Farm. This increase in plant fitness did however indicate an induced defence response by these plants to feeding, a response designed to lessen the effects of agent feeding. Headspace volatile analysis was used to investigate any volatile chemical responses by L. camara due to F. intermedia feeding at two of the five localities chosen: East London and Whitney Farm. There was no significant difference in headspace volatiles emitted by leaves of plants from the East London insect infested and control treatment plants. On the Whitney Farm damaged plants however there was a 2.5 fold increase in the emission intensity of one of the three main compounds, later identified as Beta-caryophyllene. Three major chemical constituents which were found to be common to leaf volatiles of the two varieties were identified through gas chromatography-mass spectrometry (GC-MS) from the damaged and undamaged leaves of these two varieties. The methods used in collecting leaf volatiles were shown to be significant in the strength of chromatogram peaks. Using general authentication methods and purified standards, one of these was identified as the sesquiterpene, Beta-caryophyllene (C₁₅H₂₄). This compound is one of the major constituents found in isolations of L. camara varieties worldwide. This is the first such work done on a variety of L. camara in South Africa, and hopefully the beginning of more in-depth studies of the volatile organic chemicals from the numerous naturalised varieties of L. camara. It is suggested that the sum of these responses may play a role bigger than is currently understood in this plant-insect relationship. It is also argued that feeding induced plant defences may play an important role in attempts to control alien plants using insect agents.
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- Date Issued: 2010
When birthing makes the news : the depiction of women as a newsworthy item in Die Burger (Oos-Kaap)
- Authors: Preller, Cindy
- Date: 2010
- Subjects: Mass media and women -- South Africa Childbirth -- South Africa Women -- South Africa Die Burger (Port Elizabeth, South Africa) Journalism -- South Africa -- 21st century Mass media -- Moral and ethical aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3480 , http://hdl.handle.net/10962/d1002935
- Description: The thesis “When birthing makes the news: the depiction of women as a newsworthy item in Die Burger (Oos-Kaap)” analyses a common, yet complex news topic in the South African print media due to the sensitive, often sensationalised, nature of the topic. The private experience of birthing is featured more and more in the public domain of newspapers because of widespread service delivery problems within the South African health department. Focussing on the Eastern Cape, I examine the representation of birthing in Die Burger (Oos-Kaap) in texts printed between 2005 and 2007, and scrutinise the media’s monitorial role of a self-appointed public hero acting on behalf of the women, to expose the poor conditions at government hospitals, specifically in the Nelson Mandela Bay region. How the women and their bodies are reported on, creates a discursive tension between the negative portrayals of the birthing women and the monitorial role of the media. The news values of sensationalism and profit are achieved with visceral representations of the reproductive functions of the birthing women. A poststructuralist feminist theoretical framework reveals discourses that perpetuate race, class and gender inequalities in the apparently socially-concerned sample of texts. A Critical discourse analysis (CDA) provides an approach and method to inform a close textual analysis of both the lexical and visual elements of the texts. The discourses in the sample differed from text to text. Despite these differences, the monitorial role of the media is still achieved. My research argues that acting in the public interest with sensationalist copy is still acting in the public interest. I conclude that it is not easy for newspapers to separate sensationalism from accountability. Media practitioners should be aware of their role in constructing women’s identities and be particularly thoughtful when reporting on birthing. In doing so, this research aims to improve the manner in which women and their bodies are reported on within the news industry.
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- Date Issued: 2010
Operational risk model for MSES :impact on organisational information communication technology
- Authors: Bayaga, Anass
- Date: 2011
- Subjects: Risk management -- Statistical methods Computer networks -- Security measures Risk assessment
- Language: English
- Type: Thesis , Masters , M Comm
- Identifier: http://hdl.handle.net/10353/8332 , vital:32270
- Description: The aim of the study was to investigate the impact of Information Communication Technology Operational Risk Management (ICT ORM) on the performance of a Medium Small Enterprise (MSE). The study was based upon a survey design to collect the primary data from 107 respondents using simple random sampling. The research instrument was administered online. A one stage normative model, associative in nature, was developed based upon reviewing previous research and in line with the research findings. The model elicited five factors based upon the multiple regression analysis of the data: principal causes of ORM failure related to ICT; change management requirements and ICT risk; characteristic(s) of information; challenges posed by ORM solutions and evaluation models affecting ICT adoption within MSEs. Based on the methodologies used in this study including factor analysis and multivariate regression analysis, it is recommended that this model be applied to monitor these changes more closely and to measure the changing strategies and the associated factors such as insufficient or improper user participation in systems development process, identified as potential barriers to the effective adoption and implementation of ICT within an MSE.
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- Date Issued: 2011
A role for heat shock protein 90 (Hsp90) in fibronectin matrix dynamics
- Authors: O'Hagan, Kyle Leonard
- Date: 2013
- Subjects: Molecular chaperones , Heat shock proteins , Metastasis , Cancer -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4157 , http://hdl.handle.net/10962/d1018260
- Description: To date, a significant portion of research has been devoted to understanding the biological role of the molecular chaperone, heat shock protein 90 (Hsp90), in cancer development and metastasis. Studies have alluded to over 300 clients for intracellular Hsp90, many of which are involved in oncogenic signaling pathways, making Hsp90 a bone fide drug target with several inhibitors already in clinical trials. In recent years, a limited number of extracellular Hsp90 clients have been elucidated with roles in cancer cell migration and invasion. Examples of such clients include matrix metalloproteinase-2 (MMP-2), LRP-1/CD91 and HER-2. Inhibition of extracellular Hsp90 using cellimpermeable inhibitors has been shown to reduce cancer cell migration and metastasis by a hitherto undefined mechanism. Using surface biotinylation and an enzyme linked immunosorbent assay, we provided evidence to support that Hsp90 was found extracellularly in cancers of different origin, cell type and malignancy. Next, we isolated extracellular Hsp90-containing complexes from MDA-MB-231 breast cancer cells using a cell impermeable crosslinker followed by immunoprecipitation and identified by mass spectrometry that the extracellular matrix protein, fibronectin, co-precipitated with Hsp90β. This interaction between Hsp90β and fibronectin was confirmed using pull down assays and surface plasmon resonance spectroscopy with the purified proteins. The ability of exogenous Hsp90β to increase the insoluble fibronectin matrix in Hs578T breast cancer cells indicated a role for Hsp90 in fibronectin matrix stability or fibrillogenesis. Hsp90 knockdown by RNA interference or inhibition with the small molecule inhibitor, novobiocin, resulted in a dose and time-dependent reduction of the extracellular fibronectin matrix. Furthermore, novobiocin was shown to cause the internalization of a fluorescently-labeled exogenous fibronectin matrix incorporated into the extracellular matrix by Hs578T cells. This suggested endocytosis as a possible mechanism for fibronectin turnover. This was supported by the colocalization of fibronectin with key vesicular trafficking markers (Rab-5 and LAMP-1) in small, intracellular vesicles. Furthermore, treatment with the vesicular trafficking inhibitor, methyl-β-cyclodextrin, resulted in a dose-dependent recovery in the extracellular fibronectin matrix following treatment with novobiocin. Taken together, these data provided the first evidence to suggest fibronectin as a new client of Hsp90 and that Hsp90 was involved in regulating extracellular fibronectin matrix dynamics.
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- Date Issued: 2013
Characterization of some virulence and antibiotic resistance genes of Staphylococcus aureus isolated from cases of Bovine Mastitis in Nkonkobe Municipality, Eastern Cape Province, RSA
- Authors: Pekana, Abongile
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11293 , http://hdl.handle.net/10353/d1021133
- Description: Staphylococcus aureus is one of the predominant causative agents of mastitis disease in dairy herds. Mastitis disease has a negative impact in the economic losses in the dairy sector across the globe. The aim of this study is to detect some of the virulence genes in the S. aureus isolated from 400 milk samples of subclinical and clinical mastitis dairy cows in Fort Hare dairy farm and Middle Drift dairy farm in Alice in the Eastern Cape province of South Africa. In addition antibiotic resistance pattern and antibiotic resistance genes were investigated. Gram-staining, oxidase test, catalase test and API Staph kit were preliminary biochemical tests used for the identification of S. aureus isolates. The MALDI-TOF-MS was also used for further identification. Polymerase chain reaction was performed of genes encoding antibiotic resistance as well as clumping (clfA), coagulase (coa) gene, toxic shock syndrome (tsst), exfoliative toxin A and B (eta and etb), and the gene segment encoding the immunoglobulin G binding region and X region of protein gene spa. A total of 20 (5%) S. aureus strains obtained from 400 milk samples from the two farms were subjected to 16 antibiotics for antibiotic susceptibility testing. In Middle Drift dairy farm 11 (5.5%) isolates were obtained from 200 samples and 9 (4.5%) isolates were obtained in Fort Hare dairy farm from 200 samples. A large percent of the isolates were resistant to penicillin G (60%), followed by trimethoprim (60%) and tetracycline (60%), trimethoprim-sulfamethaxazole (55%), telithroprim (55%) and doxycycline (45%). Most of the isolates were sensitive to several (50-85%) antibiotics. Of the twenty isolates tested 12 samples contained the penicillin antibiotic resistance gene (blaZ gene), 8 samples contained at least one aminoglycoside-modifying enzyme gene (AME gene); the (aac(6’)/aph(2’’) gene and no amplification occurred for aph(3’)-IIIa and ant(4’)-Ia) genes. In the case of the tetracycline antibiotic resistance gene (tetK and tetM), 2 samples contained tetM and a single sample contained tetK gene. No amplification was observed for the erythromycin antibiotic resistance genes (ermA, ermB, ermC, Mef and msrA). All the samples tested were negative for the expression of toxic syndrome gene (tsst), etb, and Immunoglobulin G binding region. However, amplification of the clumping factor was observed in 7 (35%) isolates of S. aureus, exfoliative toxin (eta) expressed 4(20%) isolates; coagulase gene (coa) yielded six DNA bands of six differences sizes from 16 (80%) isolates. A total of four different bands size were expressed for the spa X region from 12 (60%) isolates. The data obtained in this study suggests that poor hygienic practices and inadequate management practices are responsible for the increase in Staphylococcus aureus isolation. The high resistance of S. aureus to antibiotics and the distribution of virulence genes contribute in bovine mastitis in these farms may cause health problems in the community consuming raw milk purchased from these farms.
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- Date Issued: 2015
Photodynamic antimicrobial chemotherapy activities of porphyrin- and phthalocyanine-platinum nanoparticle conjugates
- Authors: Managa, Muthumuni Elizabeth
- Date: 2015
- Subjects: Photochemotherapy , Anti-infective agents , Porphyrins , Phthalocyanines , Platinum , Nanoparticles , Bioconjugates , Electrospinning
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4539 , http://hdl.handle.net/10962/d1017919
- Description: This work reports on the conjugation of differently shaped Pt nanoparticles (PtNPs) with ClGa(III) 5,10,15,20-tetrakis-(4-carboxyphenyl) porphyrin (1) as well as chloro - (5,10,15,20-tetrakis (4- (4- carboxy phenycarbonoimidoyl) phenyl) porphyrinato) gallium(III) (2) The work also reports on platination of dihydroxosilicon octacarboxyphthalocyanine (OH)₂SiOCPc (3) to give dihydroxosilicontris(diaquaplatinum)octacarboxyphthalocyanine (OH)₂SiOCPc(Pt)₃ (4). The resulting conjugates were used for photodynamic antimicrobial chemotherapy against S. aureus, E. coli and C. albicans. The degree of photo-inactivation is dependent on concentration of the conjugates, light dose (fluence) and illumination time. The log reduction obtained for 1 when conjugated to cubic PtNPs was 4.64 log (which indicate 99.99 percent of the bacteria have been killed), which is much higher than 3.94 log unit for 1-hexagonal PtNPs and 3.31 log units for 1-unshaped PtNPs. Complex 2 conjugated to hexagonal PtNPs showed 18 nm red shift in the Soret band when compared to 2 alone. Complex 2 and 2-hexagonal PtNPs as well showed promising photodynamic antimicrobial chemotherapy (PACT) activity against S. aureus, E. coli and C. albicans in solution where the log reduction obtained was 4.92, 3.76, and 3.95 respectively for 2-hexagonal PtNPs. The singlet oxygen quantum yields obtained were higher at 0.56 for 2-hexagonl PtNPs in DMF while that of 2 was 0.52 in the same solvent. This resulted in improved PACT activity for 2-hexagonal PtNPs compared to 2. Complex 4 showed slight blue shifting of the absorption spectrum when compared to complex 3 The antimicrobial activity of 4 were promising as the highest log reduction value was observed when compared to the porphyrin conjugates.
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- Date Issued: 2015
Synthesis, characterisation and evaluation of functionalized Lignocelluloses-clay nanocomposites for organic pollutant removal from water
- Authors: Mafukidze, Donovan M
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11356 , http://hdl.handle.net/10353/d1021296
- Description: PMPSgLig-NaMMT nanocomposites were prepared from methacryloxypropyltrimethoxysilane (MPS), lignocellulose and montmorillonite clay. The potential enhancement of organic pollutant adsorption capabilities of PMPSgLig-NaMMT nanocomposite from water through functionalization was investigated. PMPSgLig-NaMMT was functionalized by esterification and etherification using different methods so as to increase the surface hydrophobicity of the material and hence improve its compatibility with the target pollutants. Specific chemical routes specially tailored for PMPSgLig-NaMMT were established for functionalization mostly based on the common esterification (Fischer esterification) and etherification (Williamson‟s etherification) reactions. In the functionalization methods, factors such as pH environment, nanocomposite composition, nature of functionalization moiety, and use of or absence of solvents and their variations were studied. FT-IR, XRD, SEM and TGA were used to characterize the synthesized and functionalized nanoadsorbents. The techniques showed successful functionalization via esterification and etherification methods albeit to different extents, with clear retention of the material‟s original structure though there were signs of degradation with some methods. Characterization was supported by adsorption studies to validate implications and draw conclusions. The use of 1,10-phenathroline as a model organic pollutant in water in the adsorption studies showed that adsorbents conformed to monolayer adsorption following pseudo-second order kinetics for adsorption of organic pollutants accurately represented. Most importantly the studies revealed the significant impact of the nanocomposite composition on the overall absorbent performance. Adsorption studies also showed that functionalization via esterification methods gave rise to better adsorbents.
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- Date Issued: 2015
The ‘obesity epidemic’ : an analysis of representations of obesity in mainstream South African newspapers post-1997
- Authors: Malan, Chantelle Therese
- Date: 2015
- Subjects: Obesity -- South Africa , Obesity -- Press coverage -- South Africa , Obesity -- Social aspects -- South Africa , Discrimination against overweight persons -- South Africa , Social medicine -- South Africa , Agent (Philosophy) , Social control -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2892 , http://hdl.handle.net/10962/d1019751
- Description: This study of 449 newspaper articles from South Africa from 1997 provides an analysis of the representations of obesity evinced in the corpus. The research argues that obesity is overwhelmingly framed as being diseased and that there are four main refrains within this frame, namely, statistics on obesity, the naturalisation of negative assumptions about fat, the social dysfunction of fat and the use of crisis metaphors to describe fatness. This framing lends itself to representations of obesity which are raced, gendered and classed. Fat bodies are portrayed as being in deficit and fat people as lacking agency. The disproportional focus on black bodies in the corpus can be attributed to assumptions of ‘incivility’ which are premised on racial stereotypes which construct black people as being unintelligent, irrational, lacking agency and being largely dependent on others to survive. This disproportional focus on black bodies can also be understood in the context of emerging markets. This study argues that the medicalisation of obesity has contributed to many oversimplifications and contradictions in the representation of obesity in the corpus, which seem to go unquestioned, such as the conflation of weight and health, something I argue is one of the main contributors to the negative consequences of the dominant framing of obesity. Framing obesity as medicalised also promotes fat shaming and acts as a form of social control which maintains existing power relations through the use of discursive practices for the identification and control of deviants. These representations are problematic chiefly because they promote the dehumanisation of fat people, but also because that they do not promote good health as they claim to do.
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- Date Issued: 2015
A corpus-based approach to writing in German as a foreign language in the South African tertiary context
- Authors: Ortner, Gwyndolen Jeanie
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3660 , http://hdl.handle.net/10962/d1021256
- Description: German Studies students at Rhodes University have normally never studied the German language before enrolling for the first-year course and face the challenge of a fairly rapid linguistic advancement in order to engage meaningfully with the literatures and cultures of German-speaking countries. This thesis investigates the process of teaching and learning to write in a more academic way in German as a foreign language at Rhodes University, using corpus linguistic tools for both analysis and instruction. The past 20 years have shown a shift from traditional teaching methods resting on notions of an underlying prescriptive grammar, to teaching based on insights from real-life language data (Gabrielatos, 2005; Krummes & Ensslin, 2012; Sinclair, 1997) and applications of corpora to teaching and learning have shown to be highly successful in many European contexts (Aijmer, 2010; Johns, 1991; Granger, et al., 2002; Varley, 2009). In the South African context however, this is a relatively new concept with few publications on the application of corpus linguistics to language teaching (Van Rooy, 2008), and one which does not seem to have reached its full potential. A writing course was instituted whose aim was two-fold: 1. to teach learners “every-day academic” German words (TAG words) and phrases (collocations) based on German mother-tongue corpus evidence; 2. to have learners write short assignments in German at regular intervals (Homstad & Thorson, 1996; Estes, et al., 1998); both aims with the overarching objective to improve the students’ academic register in German. After the writing course, 80% of the participants perceived that their writing had improved and specifically attributed this to the corpus-based instruction received during the writing course, and regular writing in German. Quantitative data (from the learner corpus created) shows a marked improvement in the use of the collocations taught. Moreover, participants (weaker students in particular) also found that their writing in English had improved as a result of the various exercises they had to complete as part of our German writing course.
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- Date Issued: 2016