The phloem unloading and sucrose-sequestration pathway in the internodal stem tissue of the Saccharum hybrid var. NCo376
- Authors: Gerber, Jacqués
- Date: 2001
- Subjects: Saccharum , Sugarcane , Sugar -- Synthesis , Sugar growing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4194 , http://hdl.handle.net/10962/d1003763
- Description: Internodes 5-8, 10, 13 and 15 of Saccharum sp. var. NCo376 were examined for evidence of symplasmic phloem unloading of sucrose from the phloem, via the bundle sheath to the storage parenchyma. The vascular bundle possesses wellisolated phloem comprised of large diameter sieve elements and small diameter companion cells. A layer of phloem parenchyma surrounds the phloem, except where the phloem abuts the crushed protophloem. Outside this is a sclerenchymatous sheath, directly endarch to a parenchymatous bundle sheath, which is surrounded by storage parenchyma. The bundle sheath is interrupted at the centrifugal pole of the vascular bundle by a phloem fibre cap. Scanning Electron Microscopy revealed plasmodesmal fields throughout the bundle sheath and pith tissue. Transmission Electron Microscopy studies provided evidence of plasmodesmal occlusion, but not in all tissues. Aniline blue reactions under UV light indicate the presence of occluded plasmodesmal fields at the phloem parenchyma / sclerenchymatous sheath interface, and in localised regions of cells which are smaller than the surrounding storage parenchyma cells. This suggests a symplasmic transport pathway at these locations, and, based on these positive aniline blue reactions, with regulation via callose-mediated transplasmodesmal transport. Osmotic stress experiments, which included the addition of Ca2+, did not reveal further callose occlusion in the parenchyma, suggesting that the plasmodesmata in these regions may be closed via a noncallosic mechanism. Dye-coupling studies, using Lucifer Yellow (LYCH), which was iontophoretically injected following turgor-pressure equalisation, showed only rare, limited symplastic transport, usually only between the injected cell and one adjacent cell. Most injections did not result in transport of LYCH, suggesting either a lack of plasmodesmal connectivity, occlusion, or gating of any plasmodesmata present. This limited symplasmic transport, combined with the presence of occluded plasmodesmata at the phloem parenchyma / sclerenchymatous sheath interface suggests the presence of a two-domain phloem-unloading pathway. While symplastic transport may occur from the phloem to the sclerenchymatous sheath, further sucrose transport to the storage parenchyma appears to proceed apoplasmically from the sclerenchymatous sheath / bundle sheath interface, and into storage parenchyma cells across the cell wall and cell membrane via specialised sucrose transporters.
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- Date Issued: 2001
The vegetation potential of natural rangelands in the mid-Fish River Valley, Eastern Cape, South Africa: towards a sustainable and acceptable management system
- Authors: Birch, Natalie Vivienne Evans
- Date: 2001
- Subjects: Vegetation and climate -- South Africa -- Eastern Cape Vegetation dynamics -- South Africa -- Eastern Cape Vegetation surveys -- South Africa -- Eastern Cape Land -- South Africa -- Eastern Cape Rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4186 , http://hdl.handle.net/10962/d1003754
- Description: Desertification is the diminution or destruction of the biological potential of land, and can lead ultimately to desert-like conditions. The vegetation of southern Africa is claimed to have altered over the past 100 years and much of the change is attributed to pastoral practice. In recent years however there has been much debate around the issue of the deterioration and loss of productivity of the natural rangelands, specifically those under communal management. It is one thing to claim that the vegetation has changed but quite another to produce data and analyses to show this unequivocally. Furthermore it is generally difficult to determine the nature and extent of change in natural ecosystems, as one does not know what the optimal base-line conditions should be. For this reason emphasis has been placed on developing models of potential or expected vegetation. By comparing a model of potential or expected vegetation with that of the contemporary vegetation, areas that deviate from expectation can be identified, in so doing providing evidence of the direction of change in the rangelands under various management treatments. The objective of this study was to determine shifts in the vegetation under different land-use treatments, by developing a technique to predict the potential vegetation of an area. In order to explore the nature and extent of degradation at the landscape scale a study site was selected where a range of land-use and rangeland management practices could be studied in parallel. The mid-Fish River valley consists of three markedly different units of land management, namely commercial rangelands, communal rangelands and nature conservation areas. The vegetation within the mid-Fish River valley falls within the Thicket biome and consists of three main vegetation types namely, Short Succulent Thicket, Medium Succulent Thicket and Mesic Bushclump Savanna. The creation of this potential vegetation model was dependent on the direct gradient analysis approach of relating the community patterns with environmental variables. To achieve this, floristic information was collected at sites along a topographical-moisture gradient. A Canonical Correspondence Analysis (CCA) between the environmental variables and the plant communities produced a classification from which the conditions normally associated with the major plant communities were predicted. When projected as a digital map, the qualifying sites provided a testable hypothesis of the potential vegetation. The results of this study showed a definite grazing gradient, which reflects a change from a more mesic environment towards a more arid environment with an increase in utilisation pressure. The predictive vegetation model proved to be useful for predicting the occurrence of the valley thicket communities within the Eastern Cape.
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- Date Issued: 2001
Vein structure in relation to phloem loading in selected Ranunculaceae, Apocynaceae and Asclepiadaceae of the Eastern Cape
- Authors: Buswell, Alison Mary
- Date: 2001
- Subjects: Ranunculaceae Apocynaceae Asclepiadaceae Phloem Plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4243 , http://hdl.handle.net/10962/d1007277
- Description: The relationship between leaf architecture, vein anatomy and phloem ultrastructure, and that of possible routes from mesophyll cells to phloem and potential phloem loading method was investigated using species adapted to the southern African climate. The research was based on the hypothesis of Gamaiei and Van Bel, using nothern hemisphere species only (Gamalei 1985a, b, 1989, 1991, VanBeletal. 1988, Van Bel 1992, 1994, 1996, Van Bel & Gamalei 1991, 1992, Gamalei et al. 1992, 1994, 1996, Van Bel 1992a-c, 1993a, b, 1996). The thesis commenced with a survey ofleafarchitecture of the Ranunculaceae, Apocynaceae and Asclepiadaceae. Anatomical and ultrastructural studies followed. Leaf architecture was described according to Hickey (1973). Within the Ranuncuiaceae, leaf architecture was found to be marginally actinodromous. Venation pattern consisted of a widely spaced reticulum of delicate veins, especially in Ranunculus. Leaf architecture of the Apocynaceae was described as pinnate, camptodromous and brochidodromous. The Asclepiadaceae showed less uniformity in terms of leaf architecture, being pinnate and camptodromous, with mostly brochidodromous and, unexpectedly, eucamptodromous patterns of secondary venation. A predominantly common leaf architecture supported the move to amalgamate the two families. As the less advanced eucamptodromous arrangement could represent a more primitive branch of this huge family, the phylogenetic classification of the new amalgamated family is eagerly awaited for discussion. Allocation of vein order allowed comparisons between species and families to be drawn. Reticulum density and vein order anatomy was used to indicate potential routes from mesophyll to phloem. A definite contrast was obvious between the loose arrangement of mesophyll and veins in the mesic Ranunculus, and the close mesophyll and dense venation of the xeric apocynate and asclepiad species, and was related to habitat. Ultrastructural characteristics of companion cells, together with plasmodesmatal abundance, were considered especially important for the determination of minor vein configuration. Descriptions of plasmodesmatal distribution did not consider functional status. In this thesis, vein structure and ultrastructure were considered in relation to phloem loading, not as a demonstration thereof. All three families were designated minor vein configuration type 2a. Two interesting examples that did not adhere to the familial norm, viz. few plasmodesmata and normal companion cells, occurred in the Asclepiadaceae. Secamone alpinii had abundant aggregated plasmodesmata, forming a potential symplasmic continuum from mesophyll to companion cells. The question of plasmodesmatal functionality remained open. Ceropegia carnosa showed folding of the companion cell membrane, but no accompanying wall ingrowths. The folds were suggested to increase surface area for apoplasmic phloem loading in the noted absence of plasmodesmata. Loading routes and methods suggested were based on anatomical and ultrastructural evidence only. Whilst these results were supported by published data for other species of these families, the prediction of the Gamalei and Van Bel hypothesis did not hold true. The relatively primitive Ranunculaceae were expected to have the least advanced type 1 minor vein configuration, with abundance plasmodesmata providing a symplasmic phloem loading pathway. The relatively advanced Apocynaceae and Asc1epiadaceae were predicted to have the most progressive minor vein configuration, type 2b, with specialised transfer cells to maximise apoplasrnic uptake. As families with type 2a minor vein configurations, the Ranunculaceae were more advanced than expected and the Apocynaceae and Asc1epiadaceae less so.
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- Date Issued: 2001