A P2P middleware design for digital access nodes in marginalised rural areas
- Authors: Wertlen, Ronald
- Date: 2010
- Subjects: Software design , Rural development -- South Africa -- Eastern Cape , Rural development projects -- South Africa -- Eastern Cape , Sustainable development -- Simulation methods , Computer networks , Data transmission systems , Electronic data processing -- Distributed processing , Middleware -- Development , Computer software
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11391 , http://hdl.handle.net/10353/254 , Software design , Rural development -- South Africa -- Eastern Cape , Rural development projects -- South Africa -- Eastern Cape , Sustainable development -- Simulation methods , Computer networks , Data transmission systems , Electronic data processing -- Distributed processing , Middleware -- Development , Computer software
- Description: This thesis addresses software design within the field of Information and Communications Technology for Development (ICTD). Specifically, it makes a case for the design and development of software which is custom-made for the context of marginalised rural areas (MRAs). One of the main aims of any ICTD project is sustainability and such sustainability is particularly difficult in MRAs because of the high costs of projects located there. Most literature on ICTD projects focuses on other factors, such as management, regulations, social and community issues when discussing this issue. Technical matters are often down-played or ignored entirely. This thesis argues that MRAs exhibit unique technical characteristics and that by understanding these characteristics, one can possibly design more cost-effective software. One specific characteristic is described and addressed in this thesis – a characteristic we describe here for the first time and call a network island. Further analysis of the literature generates a picture of a distributed network of access nodes (DANs) within such network islands, which are connected by high speed networks and are able to share resources and stimulate usage of technology by offering a wide range of services. This thesis attempts to design a fitting middleware platform for such a context, which would achieve the following aims: i) allow software developers to create solutions for the context more efficiently (correctly, rapidly); ii) stimulate product managers and business owners to create innovative software products more easily (cost-effectively). A given in the context of this thesis is that the software should use free/libre open source software (FLOSS) – good arguments do also exist for the use of FLOSS. A review of useful FLOSS frameworks is undertaken and several of these are examined in an applied part of the thesis, to see how useful they may be. They form the basis for a walking skeleton implementation of the proposed middleware. The Spring framework is the basis for experiments, along with Spring-Webservices, JMX and PHP 5’s web service capabilities. This thesis builds on three years of work at the Siyakhula Living Lab (SLL), an experimental testbed in a MRA in the Mbashe district of the Eastern Cape of South Africa. Several existing products are deployed at the SLL in the fields of eCommerce, eGovernment and eLearning. Requirements specifications are engineered from a variety of sources, including interviews, mailing lists, the author’s experience as a supervisor at the SLL, and a review of the existing SLL products. Future products are also investigated, as the thesis considers current trends in ICTD. Use cases are also derived and listed. Most of the use cases are concerned with management functions of DANs that can be automated, so that operators of DANs can focus on their core business and not on technology. Using the UML Components methodology, the thesis then proceeds to design a middleware component architecture that is derived from the requirements specification. The process proceeds step-by-step, so that the reader can follow how business rules, operations and interfaces are derived from the use cases. Ultimately, the business rules, interfaces and operations are related to business logic, system interfaces and operations that are situated in specific components. The components in turn are derived from the business information model, that is derived from the business concepts that were initially used to describe the context for the requirements engineering. In this way, a logical method for software design is applied to the problem domain to methodically derive a software design for a middleware solution. The thesis tests the design by considering possible weaknesses in the design. The network aspect is tested by interpolating from formal assumptions about the nature of the context. The data access layer is also identified as a possible bottleneck. We suggest the use of fast indexing methods instead of relational databases to maintain flexibility and efficiency of the data layer. Lessons learned from the exercise are discussed, within the context of the author’s experience in software development teams, as well as in ICTD projects. This synthesis of information leads to warnings about the psychology of middleware development. We note that the ICTD domain is a particularly difficult one with regards to software development as business requirements are not usually clearly formulated and developers do not have the requisite domain knowledge. In conclusion, the core arguments of the thesis are recounted in a bullet form, to lay bare the reasoning behind this work. Novel aspects of the work are also highlighted. They include the description of a network island, and aspects of the DAN middleware requirements engineering and design. Future steps for work based on this thesis are mapped out and open problems relating to this research are touched upon.
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- Date Issued: 2010
Agricultural mechanization for sustainable agriculture and food security in Zimbabwe: a case of Bindura District in Mashonaland Central Province
- Authors: Chisango, Future, T
- Date: 2010
- Subjects: Farm mechanization -- Zimbabwe , Agricultural machinery -- Zimbabwe , Agricultural productivity -- Zimbabwe , Sustainable agriculture -- Zimbabwe , Food supply -- Zimbabwe , Agriculture and state -- Zimbabwe
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11156 , http://hdl.handle.net/10353/348 , Farm mechanization -- Zimbabwe , Agricultural machinery -- Zimbabwe , Agricultural productivity -- Zimbabwe , Sustainable agriculture -- Zimbabwe , Food supply -- Zimbabwe , Agriculture and state -- Zimbabwe
- Description: A cardinal development goal of the Zimbabwean government is agricultural mechanization through the acquisition and use of tractors by arable crop farmers in communal and resettlement state land. This research project therefore aimed at investigating the impact of mechanization on agricultural productivity focusing on farmers in Bindura district of Zimbabwe who benefitted under the ongoing farm mechanization program. The existing land policy and the issue of technical efficiency in agricultural productivity are assumed to be the drivers of the program. It is likely that these key issues will constitute an important consideration in determining the sustainability of the mechanization policy. A multistage sampling technique was used to randomly select 90 farmers in the study area. The Cobb Douglas approach and Logistic regression were used to analyze data obtained from the respondents. Results revealed that tractor use was positively influenced by household size, access to extension services and crop output equivalent. Education, land area cultivated, stoniness negatively influenced the probability of adoption of mechanized farming. Furthermore, the technical efficiency estimate of adopter and nonadopters of mechanized farming showed no difference in their level of technical efficiency in agricultural productivity that was 64 percent on average. The level of observed inefficiency was increased by slope, stoniness and household size while age reduced technical inefficiency. It is recommended that government should consolidate the present gains arising from extension services. Also, environmental factors such as slope (topography) and stoniness, which constituted major disincentives in communal areas, could be overcome if government and farmers can identify and open up new areas of farmland for occupation by farmers.
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- Date Issued: 2010
An analysis of groundwater in Mjinchi District of Central Malawi
- Authors: Mleta, Prince W C
- Date: 2010
- Subjects: Groundwater -- Malawi , Water-supply -- Malawi
- Language: English
- Type: Thesis , Masters , MSc (Geography)
- Identifier: vital:11510 , http://hdl.handle.net/10353/366 , Groundwater -- Malawi , Water-supply -- Malawi
- Description: Groundwater resources is the major source of potable water in Mchinji District of Central Malawi and globally. Although the total amount of water on and under the earth‟s surface is generally assumed to have remained constant, the rapid population boom coupled with extension of agricultural farmland and industrial development are putting pressure and stress on the quality and quantity of water resources. In principal therefore, „use and discard‟ philosophy in water resources cannot be subscribed (Lloyd, 1999). The situation ultimately calls for rational management of water resources to ensure its sustainability. Water intended for human consumption must be free from organisms that are the causative agents of diseases and must not contain chemical substances at concentrations that may be hazardous to human health. In addition drinking water should be aesthetically acceptable, free from unpleasant taste, color, odor and turbidity. Drinking water should also be free from bacteria and viruses whose presence would indicate fecal contamination. Some are known to be toxic and their concentrations must be below acceptable value, taking into account that drinking water is but one of the several pathways by which substances enters the body. ix Due to perceived concerns of over extraction and rapid water resources depletion, optimal management of groundwater resources are now receiving much attention. Their associated literatures have taken quite different approaches both philosophically and operationally to the analysis of how groundwater should be managed, allocated and cared for spatially and temporally. This study investigated the spatial and temporal fluctuations of concentrations of chemical and biological substances in groundwater such as pH, electrical conductivity, total dissolved solids, iron, fluoride, manganese, chloride, sulfates, sodium and fecal coliform in Mchinji District of Central Malawi. Through examination and analysis of static water levels as an indicator of water table fluctuations, groundwater availability was also measured. It was found that anthropogenic activities on the landscape can impact the quality and quantity of the water resources in this area and this impact on the various sectors of the inhabitant‟s livelihoods. Groundwater in Mchinji is composed of a number of chemical and biological elements whose origin is either from the material in which it percolates through, or stored before exploitation. Anthropogenic activities in this area plays a role in the quality and quantity of groundwater through land use and land cover change as evidenced by comparisons of Landsat Thematic x Mapper™ satellite images over different time scales. In Mchinji no regular groundwater monitoring is being done indicating a deficiency in sustainability interventions of the resource. This study calls for integrated and sustainable water resources management and coordinated efforts amongst water users, local councils, regulatory authorities and environmental policy makers. Of far greater importance in groundwater sustainability analysis is the issue of groundwater monitoring. It is imperative therefore to preserve the resource while preservation is still possible. Groundwater is now turning into „blue gold‟ and becoming a highly sought-after commodity. It should, however, be utilized sustainably to meet the needs of the present without compromising the ability of future generations to meet their own needs.
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- Date Issued: 2010
Application of the sustainable livelihoods framework to the analysis of the Provincial Growth and Development Plan of the Eastern Cape: a case study of the Massive Food Production Programme in Nkonkobe Municipality and Buffalo City Municipality
- Authors: Dirwayi, Tafadzwa P
- Date: 2010
- Subjects: Sustainable agriculture -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Poverty -- South Africa -- Eastern Cape , Rural poor -- South Africa -- Eastern Cape , Food supply -- South Africa -- Eastern Cape , Food security -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11159 , http://hdl.handle.net/10353/353 , Sustainable agriculture -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Poverty -- South Africa -- Eastern Cape , Rural poor -- South Africa -- Eastern Cape , Food supply -- South Africa -- Eastern Cape , Food security -- South Africa -- Eastern Cape
- Description: The Government of the Eastern Cape Province introduced the Massive Food Production programme, which is a cornerstone within the agrarian reform initiative of the Provincial Growth and Development Plan (PGDP). The programme has been going on for more than seven years, however little has been documented on its impact. The main objective of the study was to investigate the impact of the Massive Food Production Programme on the livelihoods in Nkonkobe Municipality. This study did not only investigate its impact on the participants but also assessed its impact on the recipient communities. Accordingly, the specific objectives of the study were to investigate the impact of the Massive Food Programme on the asset base of participants, the general livelihood activities, and the livelihood outcomes. Among other livelihood activities, the study made an in-depth investigation on the impact of the Massive Food Production Programme on maize crop production. This is because the Massive Food Production was aimed at maize crop production. Previous studies investigated on the indicators of success that can be used to measure the impact of this programme. After the wide-ranging evaluation of views, arguments and research findings, a model to measure impact of the programme was designed. The asset base improvement was used as the proxy of impact. Nine factors were selected from the principal component analysis of the many factors that were taken to affect participation. Three other dummy variables to proximate location, participation and group turnover were added to the regression model that was developed to measure impact. The DFID Sustainable Livelihood Framework was used to investigate the impact of the Massive Food Production Programme. This approach was used in both conceptualizing the study and the selection of variables. The DFID Sustainable Livelihood Approach was selected because unlike the CARE or UNDP Sustainable Livelihood models, it was designed for such purposes. Data collection was accomplished through observation, interviewing, and focus group discussions. The researcher also made use of project annual reports on change of livelihoods, baseline survey reports, project log frame, internal reports, work plans, budgets and mid-term evaluation reports as sources of secondary data. v The research findings were analysed using several analytical procedures, including the conventional descriptive statistics, principal components analysis, and linear regression analysis. The use of the different types of analysis was driven by the research questions under investigation and the theories on which they are based, and by the available data. The study revealed that to some extent the Massive Food Production Programme has managed to improve the asset base of the farmers. However, its impact on ensuring food security is still debatable. Findings of the study revealed that most of the participants and the non-participants communities experienced food shortages in the last season 2007/8 for at most three months. The study revealed that the highest agricultural income is from livestock sales. The varying locations showed the potential of livestock production. It is recommended that development agencies consider livestock production as an agricultural strategy with immense potential for enhancing sustaining rural livelihoods
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- Date Issued: 2010
Assessment of antibiotic production by some marine Streptomyces isolated from the Nahoon Beach
- Authors: Ogunmwonyi, Isoken Nekpen Henrietta
- Date: 2010
- Subjects: Streptomyces , Actinobacteria , Gram-positive bacteria , Antibiotics , Antibiotics -- Testing , Drug resistance in microorganisms
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11243 , http://hdl.handle.net/10353/264 , Streptomyces , Actinobacteria , Gram-positive bacteria , Antibiotics , Antibiotics -- Testing , Drug resistance in microorganisms
- Description: Rapidly emerging strains of bacteria resistant to most advanced antibiotics have become issues of very important public health concern. Research currently directed towards marine actinomycetes presents a vast potential for new compounds that could be able to safely and effectively target resistant species. In this regard, ten putative Streptomyces strains isolated from the Nahoon beach were selected and assessed for antibiotic production and activity against a wide range of bacteria including reference strains, environmental strain and clinical isolates. The ethyl acetate extracts of the putative Streptomyces isolates showed activities against at least 6 and up to 26 of the 32 test bacteria. Inhibition zones were found to range between 9-32 mm diameters at a concentration of 10 mg/ml. The minimum inhibitory concentrations (MICs) of the crude extracts ranged from 0.039 - 10 mg/ml and the least minimum bactericidal concentration (MBC) demonstrated was 0.625 mg/ml against a reference strain Staphylococcus aureus ATCC 6538. Time kill kinetics of all extracts revealed bacteristatic and bactericidal activities. Average Log reductions in viable cell counts for all the extracts ranged from 0.86 Log10 and 3.99 Log10 cfu/ml after 3 h interaction and 0.01 Log10 and 4.86 Log10 after 6 h interaction at MIC, 2 × MIC, 3 × MIC and 4 × MIC concentrations. Most of the extracts were speedily bactericidal at 3 × MIC and 4 × MIC resulting in over 50 % elimination of most of the test bacteria within 3 h and 6 h interaction. The partial characterization of the crude extracts by IR spectral analysis revealed possibility of terpenoid, long chain fatty acids and secondary amine derivatives compounds in the extracts. It is therefore recommended that further investigation should address the relationship between the structure of the active component of the extracts and the broad spectrum activity, as well as a rapid method for large scale production and purification and whether this group of antibiotics has any application in managing human infectious disease.
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- Date Issued: 2010
Assessment of bioflocculant production by some marine bacteria isolated from the bottom sediment of Algoa Bay
- Authors: Cosa, Sekelwa
- Date: 2010
- Subjects: Flocculants , Bacteria -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11244 , http://hdl.handle.net/10353/404 , Flocculants , Bacteria -- South Africa -- Algoa Bay
- Description: Several problems concerning the use of conventional synthetic flocculants has necessitated the need for alternative cost effective, safe and efficient bioflocculants from microorganisms inhabiting many environments, particularly those from unusual environments. Hence, this study assessed bioflocculant production by three marine bacteria isolated from the bottom sediment of Algoa Bay in the Eastern Cape Province of South Africa. Analysis of the 16S rDNA sequences led to their identification as Halobacillus sp. Mvuyo, Virgibacillus sp. Rob and Oceanobacillus sp. Pinky. Several factors affecting the production and activity of the bioflocculant(s) were studied. Halobacillus sp. Mvuyo produced bioflocculant optimally with glucose (76%) and ammonium chloride (93%) as sole carbon and nitrogen sources, respectively and at neutral pH and in the presence of Ca2+. On the other hand, Virgibacillus sp. Rob preferred glucose (70.4 %) and iron sulphate (74%) as carbon and nitrogen source respectively; an alkaline pH (12.0) and Fe2+. Oceanobacillus sp. Pinky produced bioflocculant optimally when sucrose (80%) and peptone (72.4 %) were used as carbon and nitrogen source respectively, at neutral pH, and in the presence of Ca2+ cation. The chemical analyses of the partially purified bioflocculants revealed that the bioflocculants produced by Halobacillus sp. Mvuyo and Oceanobacillus sp. Pinky were glycoproteins, while that produced by Virgibacillus sp. Rob was a polysaccharide. We thus conclude that Halobacillus sp. Mvuyo, Virgibacillus sp. Rob and Oceanobacillus sp. Pinky hold promise as producers of new and efficient bioflocculant(s). We recommended development of process conditions for large scale production of the bioflocculants followed by their detailed characterization, as well as pilot scale assessment of the applicability of the purified bioflocculant in water/wastewater treatment and other industrial uses
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- Date Issued: 2010
Assessment of plants used for the treatment of cattle wounds and myiasis in Amatola Basin, Eastern Cape Province, South Africa
- Authors: Soyelu, Oluseyi Temitope
- Date: 2010
- Subjects: Domestic animals -- Parasites , Insect pests -- Control , Cattle -- Health -- South Africa -- Eastern Cape , Screwworm -- Biological control , Traditional medicine -- South Africa -- Eastern Cape , Medicinal plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11160 , http://hdl.handle.net/10353/d1000986 , Domestic animals -- Parasites , Insect pests -- Control , Cattle -- Health -- South Africa -- Eastern Cape , Screwworm -- Biological control , Traditional medicine -- South Africa -- Eastern Cape , Medicinal plants -- South Africa -- Eastern Cape
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- Date Issued: 2010
Assessment of the prevalence of virulent Eschericia coli strains in the final effluents of wastewater treatment plants in the Eastern Cape Province of South Africa
- Authors: Osode, Augustina Nwabuje
- Date: 2010
- Subjects: Escherichia coli , Escherichia coli -- Genetics , Effluent quality -- Testing , Water -- Purification , Sewage disposal plants , Escherichia coli -- South Africa -- Eastern Cape , Whole effluent toxicity testing
- Language: English
- Type: Thesis , Doctoral , PhD (Microbiology)
- Identifier: vital:11246 , http://hdl.handle.net/10353/d1001062 , Escherichia coli , Escherichia coli -- Genetics , Effluent quality -- Testing , Water -- Purification , Sewage disposal plants , Escherichia coli -- South Africa -- Eastern Cape , Whole effluent toxicity testing
- Description: Escherichia coli (E. coli) is a common inhabitant of surface waters in the developed and developing worlds. The majority of E. coli cells present in water are not particularly pathogenic to humans; however, there are some present in small proportion that possess virulence genes that allow them to colonize the digestive tract. Pathogenic E. coli causes acute and chronic diarrheal diseases, especially among children in developing countries and in travelers in these locales. The present study, conducted between August 2007 and July 2008, investigated the prevalence and distribution of virulent E. coli strains as either free or attached cells in the final effluents of three wastewater treatment plants located in the Eastern Cape Province of South Africa and its impact on the physico-chemical quality of the receiving water body. The wastewater treatment plants are located in urban (East Bank Reclamation Works, East London), peri-urban (Dimbaza Sewage Treatment Works) and in rural area (Alice Sewage Treatment Works). The effluent quality of the treatment plants were acceptable with respect to pH (6.9-7.8), temperature (13.8-22.0 °C), dissolved oxygen (DO) (4.9-7.8 mg/L), salinity (0.12-0.17 psu), total dissolved solids (TDS) (119-162 mg/ L) and nitrite concentration (0.1-0.4 mg/l). The other xii physicochemical parameters that did not comply with regulated standards include the following: phosphate (0.1-4.0 mg/L); chemical oxygen demand (COD) (5-211 mg/L); electrical conductivity (EC) (237-325 μS/cm) and Turbidity (7.7-62.7 NTU). Results suggest that eutrophication is intensified in the vicinity of the effluent discharge points, where phosphate and nitrate were found in high concentrations. Presumptive E. coli was isolated from the effluent samples by culture-based methods and confirmed using Polymerase Chain Reaction (PCR) techniques. Antibiogram assay was also carried out using standard in vitro methods on Mueller Hinton agar. The viable counts of presumptive E. coli for the effluent samples associated with 180 μm plankton size ranged between 0 – 4.30 × 101 cfu/ml in Dimbaza, 0 – 3.88 × 101 cfu/ml in Alice and 0 – 8.00 × 101 cfu/ml in East London. In the 60 μm plankton size category E. coli densities ranged between 0 and 4.2 × 101 cfu/ml in Dimbaza, 0 and 2.13 × 101 cfu/ml in Alice and 0 and 8.75 × 101 cfu/ml in East London. Whereas in the 20 μm plankton size category presumptive E. coli density varied from 0 to 5.0 × 101 cfu/ml in Dimbaza, 0 to 3.75 × 101 cfu/ml in Alice and 0 to 9.0 × 101 cfu/ml in East London. The free-living presumptive E. coli density ranged between 0 and 3.13 × 101 cfu/ml in Dimbaza, between 0 and 8.0 × 101 cfu/ml in Alice and between 0 and 9.5 × 101 cfu/ml in East London. Molecular analysis successfully amplified target genes (fliCH7, rfbEO157, ial and aap) which are characteristic of pathogenic E. coli strains. The PCR assays using uidA-specific primer confirmed that a genetic region homologous in size to the E. coli uidA structural gene, including the regulatory region, was present in 3 of the E. coli isolates from Alice, 10 from Dimbaza and 8 from East London. Of the 3 E. coli isolates from Alice, 1 (33.3%) was positive for the fliCH7 genes and 3 was positive for rfbEO157 genes. Out of the 10 isolates from Dimbaza, 4 were xiii positive for fliCH7 genes, 6 were positive for the rfbEO157 genes and 1 was positive for the aap genes; and of the 8 isolates from East London, 1 was positive for fliCH7 genes, 2 were for the rfbEO157 genes, 6 were positive for the ial genes. Antimicrobial susceptibility profile revealed that all of the E. coli strains isolated from the effluent water samples were resistant (R) to linezolid, polymyxin B, penicillin G and sulfamethoxazole. The E. coli isolates from Dimbaza (9/10) and East London (8/8) respectively were resistant to erythromycin. All the isolates were found to be susceptible (S) to amikacin, ceftazidime, ciprofloxacin, colistin sulphate, ceftriaxone, cefotaxime, cefuroxime, ertapenem, gatifloxacin, gentamycin, imidazole, kanamycin, meropenem, moxifloxacin, neomycin, netilmicin, norfloxacin and tobramycin. The findings of this study revealed that the Alice wastewater treatment plant was the most efficient as it produced the final effluent with the least pathogenic E. coli followed by the Dimbaza wastewater treatment plant. In addition, the findings showed that the wastewater treatment plant effluents are a veritable source of pathogenic E. coli in the Eastern Cape Province watershed. We suggest that to maximize public health protection, treated wastewater effluent quality should be diligently monitored pursuant to ensuring high quality of final effluents.
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- Date Issued: 2010
Building a semantic web-based e-health component for a multipurpose communication centre
- Authors: Hlungulu, Bulumko
- Date: 2010
- Subjects: Rural development -- South Africa -- Eastern Cape , Information technology -- South Africa -- Eastern Cape , Internet in medicine -- South Africa -- Eastern Cape , Telecommunication in medicine -- South Africa -- Eastern Cape , Health -- Computer network resources
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11380 , http://hdl.handle.net/10353/374 , Rural development -- South Africa -- Eastern Cape , Information technology -- South Africa -- Eastern Cape , Internet in medicine -- South Africa -- Eastern Cape , Telecommunication in medicine -- South Africa -- Eastern Cape , Health -- Computer network resources
- Description: Rural communities have limited access to health information which is made available on the internet. This is due to poor infrastructure (i.e., lack of clinics or Internet access) and that gives them problems in accessing information within the domain of health. The availability of Information and Communication Technologies (ICTs) in a rural community can provide the community with a number of beneficial solutions to their problems as they maximize the potential of knowledge sharing and delivery. This research seeks to make use of ICTs deployed in the community of Dwesa, in order to contribute to improving the health standards of the community. It seeks to accomplish this by carrying out an investigation and literature review with the aim of understanding health knowledge sharing dynamics in the context of marginalized communities. The knowledge acquired will then be used in the development and implementation of a semantic web-based e-Health portal as part of the Siyakhula Living Lab (SLL) project. This portal will share and deliver western medical knowledge, traditional knowledge and indigenous knowledge. This research seeks to make use of a combination of Free and/or Open Sources Software in developing the portal to make it affordable to the community.
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- Date Issued: 2010
Chemical transformations and phytochemical studies of bioactive components from extracts of Rosmarinus officinalis L
- Authors: Okoh, Omobola Oluranti
- Date: 2010
- Subjects: Essences and essential oils , Rosmarinus , Lamiaceae , Solution (Chemistry) , Extractive distillation , Medicinal plants , Bioactive compounds
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11331 , http://hdl.handle.net/10353/354 , Essences and essential oils , Rosmarinus , Lamiaceae , Solution (Chemistry) , Extractive distillation , Medicinal plants , Bioactive compounds
- Description: Variations in the yield, chemical composition, antibacterial, and antioxidant properties of the essential oils of Rosmarinus officinalis L. cultivated in Alice, Eastern Cape of South Africa over a period of 12 months using the solvent-free microwave extraction and traditional hydrodistillation methods were evaluated. The GC-MS analyses of the essential oils revealed the presence of 33 compounds with 1,8-cineole, a-pinene, camphor, verbenone, bornyl acetate and camphene constituting about 80 percent of the oils throughout the period of investigation, with the solvent-free microwave extraction method generally yielding more of the major components than the hydrodistillation method. Each of the major components of the oils varied in quantity and quality of yield at different periods of the year. The method of extraction and time of harvest are of importance to the quantity and quality of essential oil of Rosmarinus officinalis. Higher amounts of oxygenated monoterpenes such as borneol, camphor, terpene- 4-ol, linalool, a-terpeneol were present in the oil of SFME in comparison with HD. However, HD oil contained more monoterpene hydrocarbons such as a-pinene, camphene, β-pinene, myrcene, a-phellanderene, 1,8-cineole, trans- β-ocimene, γ-teprinene, and cis-sabinene hydrate than SFME extracted oil. Accumulation of monoterpene alcohols and ketones was observed during maturation process of Rosmarinus leaves. Quantitative evaluation of antibacterial activity, minimum inhibitory concentration values were determined using a serial microplate dilution method. The essential oils obtained using both methods of extraction were active against all the bacteria tested at a concentration of 10 mg mL-1. The minimum inhibitory concentrations for the SFME extracted oils ranged between 0.23 and 1.88 mg mL-1, while those of the HD extracted oils varied between 0.94 and 7.5 mg mL-1, thus suggesting that the oil obtained by solvent free microwave extraction was more active against bacteria than the oil obtained through hydrodistillation. The antioxidant and free radical scavenging activity of the obtained oils were tested by means of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH+) assay and β- carotene bleaching test. In the DPPH+ assay, while the free radical scavenging activity of the oil obtained by SFME method showed percentage inhibitions of between 48.8 percent and 67 percent, the HD derived oil showed inhibitions of between 52.2 percent and 65.30 percent at concentrations of 0.33, 0.50 and 1.0 mg mL-1, respectively. In the β-carotene bleaching assay, the percentage inhibition increased with increasing concentration of both oils with a higher antioxidant activity of the oil obtained through the SFME than the HD method. Thin layer chromatography (TLC) was used to analyze the chemical composition of the extracts using three eluent solvent systems of varying polarities i. e. CEF, BEA and EMW and sprayed with vanillin-sulfuric acid. The chemical composition of the different extracts was similar with the exception of methanol and water extracts which had only one or two visible compounds after treating with vanillin-spray reagent. To evaluate the number of antibacterial compounds present in the fractions, bioautography was used against two most important nosocomial microorganisms. S. aureus (Gram positive) and E. coli (Gram negative). Nearly all the crude serial extraction fractions contained compounds that inhibited the growth of E. coli. The hexane extract had the most lines of inhibition followed by ethyl acetate. Bioassay-guided fractionation against E. coli was used to isolate antibacterial compounds. The largest number of antibacterial compounds occurred in the hexane fraction. Furthermore we tried to complete the characterization by extracting and studying other biologically important plant metabolites such as phenolic compounds to evaluate the antioxidant capacity of Rosmarinus extracts.
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- Date Issued: 2010
Citrus, labour and gender in the Eastern Cape: the case of the Kat River area
- Authors: Mzitshi, Zoleka Alice Florence
- Date: 2010
- Subjects: Citrus -- South Africa -- Eastern Cape , Citrus , Working class women -- South Africa -- Eastern Cape , Sex role -- South Africa -- Eastern Cape , Citrus fruit industry -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Geography)
- Identifier: vital:11511 , http://hdl.handle.net/10353/379 , Citrus -- South Africa -- Eastern Cape , Citrus , Working class women -- South Africa -- Eastern Cape , Sex role -- South Africa -- Eastern Cape , Citrus fruit industry -- South Africa -- Eastern Cape
- Description: Agriculture plays a significant role in South Africa’s export earning and in providing employment opportunities. Amongst the major agricultural crops is citrus. Within the context of postmodern and feminist geographies and utilizing the intensive research design, this thesis discusses citrus production with a focus on growers and black women workers in the Kat River Valley area in Fort Beaufort, Eastern Cape. The thesis also explores the challenges and implications of restructuring within agriculture for growers, cooperatives and labour within the citrus industry. It is argued that whilst legislation related to labour and tenure within agriculture has changed since the mid-1990s, power relations in the citrus industry have remained firmly entrenched. Consequently, the flexible labour strategies that the citrus farmers adopted have had serious consequences for women worker
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- Date Issued: 2010
Developing SOA wrappers for communication purposes in rural areas
- Authors: Samalenge, Jimmy
- Date: 2010
- Subjects: Computer network architectures , Computer architecture , Computer software -- Specifications , Rural development projects -- South Africa -- Eastern Cape , Web services , System design , Technological innovations -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11386 , http://hdl.handle.net/10353/320 , Computer network architectures , Computer architecture , Computer software -- Specifications , Rural development projects -- South Africa -- Eastern Cape , Web services , System design , Technological innovations -- South Africa -- Eastern Cape
- Description: The introduction of Web Services as a platform upon which applications can communicate has contributed a great deal towards the expansion of World Wide Web technologies. The Internet and computing technologies have been some of the factors that have contributed to the socio-economic improvement of urban and industrial areas. This research focuses on the application of Service-Oriented Architecture (SOA) and Web Services technologies in Information and Communication Technologies for Development (ICT4D) contexts. SOA is a style used to design distributed systems, and Web Services are some of the common realizations of the SOA. Web Services allow the exchange of data between two or more machines in a simple and standardized manner over the network. This has resulted in the augmentation of ways in which individuals in a society and in the world communicate. This research aims to develop a SOA-based system with services that are implemented as Web Services. The system is intended to support communication activities of Dwesa community members. The communication methods identified as the most commonly used in the Dwesa community are Short Message Services (SMSs) and voice calls. In this research we have identified further methods (i.e. Multimedia Message Service, Electronic mail and Instant Messaging) to augment communication activities in Dwesa. The developed system, therefore, exposes SMS Web Service, MMS Web Service, Email Web Service and IM Web Service that are consumed in machine-to-machine, machine-to-person and person-to-person types of communication. We have also implemented a one-stop communication shop, through a web portal which provides interfaces to the different communication modules. Elaborate functional and usability testing have also been undertaken to establish the viability and end-user acceptance of the system respectively. This research has provided the initial validation of the effectiveness of the SOA-based system in ICT4D contexts
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- Date Issued: 2010
Dynamic adaptive cost model for wireless Internet connectivity in African rural communities
- Authors: Sibanda, Khulumani
- Date: 2010
- Subjects: Internet , Community development -- South Africa -- Eastern Cape , Cost effectiveness , Wireless communication systems , Rural development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Computer Science)
- Identifier: vital:11389 , http://hdl.handle.net/10353/380 , Internet , Community development -- South Africa -- Eastern Cape , Cost effectiveness , Wireless communication systems , Rural development -- South Africa -- Eastern Cape
- Description: In today’s dynamic technological landscape, wireless communication networks have become an important part of economic development. The emergence of wireless technologies raises hopes to extend communication to remote areas that have not seen any tangible deployment to date. As developing nations pin their hopes to wireless technologies, cost models for wireless communication networks are becoming vital to support the emerging technologies. However, varying cost changes raise critical challenges to the estimation of both capital expenditure and operational expenditure. The network deployment process has numerous events that may cause adjustments to initially estimated project costs. These adjustments are necessary for a cost management plan and this plan includes monitoring cost performance and ensuring that only appropriate changes are made to the network project. The incidents that may cause cost changes can not be entirely predicted as their distribution tend to change dynamically from time to time. Estimating network deployment costs in such a dynamic environment necessitates cost models that can adapt to random occurrence of cost changes. Widely used cost models are usually performed by experienced personnel whose engineering experience is derived from deploying similar networks. In this approach experienced personnel add a certain percentage to the cost estimate to cater for contingency costs. Certainly such an approach depends on individual opinion, making it subjective and void of mathematical estimating relationships which are of paramount importance in ensuring that estimated deployment costs are sufficient to deal with cost uncertainties. We observe that existing approaches can only explore a limited solution space and hence can lead to cost overruns if implemented in dynamically cost changing environments. This thesis presents a wireless communication network deployment cost model that incorporates uncertainties into the final cost estimate. The model is adaptive to unpredictable cost changes since it allows adjustments of confidence levels when calculating contingency costs. This allows dynamically updating the cost changes without the cost model being reconstructed from scratch. We make use of the Poisson process in modeling the occurrence of incidents that are responsible for causing cost changes during network deployment. We also show that the occurrence of the incidents causing cost change are random and tend to follow the Poisson distribution. Using different levels of confidence we model various cost contingencies and make sensitivity analyses to identify the probability of cost overrun when given different contingencies. The dynamic adaptive cost model can be used either at the strategic level to understand the cost of a particular technique or at the operational level, as a way to show how Poisson process in network deployment can compare with engineering experience and other estimating techniques. We believe that the model is useful for remote areas where deployment costs are volatile and the distribution of incidents causing cost change to original cost estimates are diverse and dynamically changing. Further we expect that our research improves the knowledge base of information about the costs for rural communities to connect to the Internet, consequently providing useful input to future policy debates. This work is further poised to be a utility function to help those planning internet infrastructure deployments in least developed regions.
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- Date Issued: 2010
Effects of dietary supplementation on physico-chemical and consumer sensory characteristics of chevon from South African indigenous goat genotypes
- Authors: Xazela, Nomasonto Martha
- Date: 2010
- Subjects: Color of meat , Food -- Sensory evaluation , Commercial products -- Testing , Goats -- South Africa -- Eastern Cape , Goats -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11809 , http://hdl.handle.net/10353/357 , Color of meat , Food -- Sensory evaluation , Commercial products -- Testing , Goats -- South Africa -- Eastern Cape , Goats -- Feeding and feeds
- Description: The study was conducted at the University of Fort Hare farm to assess the effect of dietary supplementation on physico-chemical and consumer sensory characteristics of chevon from South African indigenous goat genotypes. Forty-eight 6-month-old Xhosa lop-eared (XLE), Nguni (NGN), Xhosa-Boer cross (XBC) and Boer (BOR) castrated goats with a body weight range from 20 to 25 kg were used in this study. Half of the goats in each genotype were supplemented with 200g/head/day of sunflower cake. The other half of goats in each genotype was not supplemented. The goats were slaughtered at day 90 to determine slaughter weight (SLW), cold dress mass (CDM), meat colour, cooking loss, meat pH and Warner-Bratzler (WB) shear force values. Furthermore, a consumer sensory evaluation of cooked or fried chevon from supplemented and non-supplemented goats was conducted with consumers of different ages, tribes and gender. The XLE and NGN goats had higher pH24 (P < 0.05) than BOR and XBC goats. Supplemented BOR goats had higher L* values than their non-supplemented counterparts (P < 0.05). The other meat quality attributes in XLE and NGN were comparable to those in meat from the Boer goat. Female respondents gave higher (P < 0.05) sensory scores than male respondents for both cooked and fried meat on aroma intensity. Shona consumers gave higher (P < 0.05) aroma intensity scores than the Xhosa and the Zulu consumers for both cooked and fried meat. In the non-supplemented goats, fried meat for all genotypes was superior (P < 0.05) to the cooked meat for initial impression of juiciness. Age and gender of respondents and thermal iv treatment influenced initial impression of juiciness scores (P < 0.05). The quality of chevon from XLE and NGN was comparable to that of the Boer goat, and dietary supplementation improved most meat quality attributes. Chevon from the supplemented goats had higher consumer sensory scores than chevon from the non-supplemented goats. Keywords: supplementation, meat colour, ultimate pH, consumer sensory evaluation, Xhosa lop eared, Nguni, Boer goats.
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- Date Issued: 2010
Effects of marketing channel on bruising, ultimate pH and colour of beef, and stakeholder perceptions on the quality of beef from cattle slaughtered at smallholder abattoir
- Authors: Vimiso, Peter
- Date: 2010
- Subjects: Beef -- South Africa -- Marketing , Beef -- Quality , Beef cattle -- Breeding -- South Africa , Beef cattle breeds -- South Africa , Color of meat , Beef industry -- South Africa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11172 , http://hdl.handle.net/10353/d1001009 , Beef -- South Africa -- Marketing , Beef -- Quality , Beef cattle -- Breeding -- South Africa , Beef cattle breeds -- South Africa , Color of meat , Beef industry -- South Africa
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- Date Issued: 2010
Effects of removing Acacia Mearnsii on the water table, soil and vegetation properties in the Tsomo Valley of the Eastern Cape Province, South Africa
- Authors: Moyo, Hloniphani Peter Mthunzi
- Date: 2010
- Subjects: Plant-water relationships , Acacia mearnsii , Water table , Wattles (Plants) -- South Africa -- Eastern Cape , Streamflow -- South Africa -- Eastern Cape , Groundwater -- South Africa -- Eastern Cape , Groundwater recharge -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Pasture Science)
- Identifier: vital:11173 , http://hdl.handle.net/10353/d1001011 , Plant-water relationships , Acacia mearnsii , Water table , Wattles (Plants) -- South Africa -- Eastern Cape , Streamflow -- South Africa -- Eastern Cape , Groundwater -- South Africa -- Eastern Cape , Groundwater recharge -- South Africa -- Eastern Cape
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- Date Issued: 2010
Efficient rectenna circuits for microwave wireless power transmission
- Authors: Teru, Agboola Awolola
- Date: 2010
- Subjects: Electric power production -- Technological innovations , Integrated circuits -- Design and construction , Electronic circuit design , Semiconductors -- Design and construction
- Language: English
- Type: Thesis , Masters , MSc (Physics)
- Identifier: vital:11590 , http://hdl.handle.net/10353/481 , Electric power production -- Technological innovations , Integrated circuits -- Design and construction , Electronic circuit design , Semiconductors -- Design and construction
- Description: Miniaturisation has been the holy grail of mobile technology. The ability to move around with our gadgets, especially the ones for communication and entertainment, has been what semiconductor scientists have battled over the past decades. Miniaturisation brings about reduced consumption in power and ease of mobility. However, the main impediment to untethered mobility of our gadgets has been the lack of unlimited power supply. The battery had filled this gap for some time, but due to the increased functionalities of these mobile gadgets, increasing the battery capacity would increase the weight of the device considerably that it would eventually become too heavy to carry around. Moreover, the fact that these batteries need to be recharged means we are still not completely free of power cords. The advent of low powered micro-controllers and sensors has created a huge industry for more powerful devices that consume a lot less power. These devices have encouraged hardware designers to reduce the power consumption of the gadgets. This has encouraged the idea of wireless power transmission on another level. With lots of radio frequency energy all around us, from our cordless phones to the numerous mobile cell sites there has not been a better time to delve more into research on WPT. This study looks at the feasibilities of WPT in small device applications where very low power is consumed to carry out some important functionality. The work done here compared two rectifying circuits’ efficiencies and ways to improve on the overall efficiencies. The results obtained show that the full wave rectifier would be the better option when designing a WPT system as more power can be drawn from the rectenna. The load also had a great role as this determined the amount of power drawn from the circuitry.
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- Date Issued: 2010
Evaluation and management of cover crop species and their effects on weed dynamics, soil fertility and maize (Zea mays L.) productivity under irrigation in the Eastern Cape Province, South Africa
- Authors: Murungu, Farayi Solomon
- Date: 2010
- Subjects: Crops -- South Africa , Conservation of natural resources -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape , Soil percolation -- South Africa -- Eastern Cape , Soil permeability -- South Africa -- Eastern Cape , Irrigation farming -- South Africa -- Eastern Cape , Corn -- South Africa -- Eastern Cape , Soil fertility -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Crop Science)
- Identifier: vital:11866 , http://hdl.handle.net/10353/335 , Crops -- South Africa , Conservation of natural resources -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape , Soil percolation -- South Africa -- Eastern Cape , Soil permeability -- South Africa -- Eastern Cape , Irrigation farming -- South Africa -- Eastern Cape , Corn -- South Africa -- Eastern Cape , Soil fertility -- South Africa -- Eastern Cape
- Description: The current interest in conservation agriculture (CA) technologies is a result of the need to reduce excessive land degradation in most crop producing areas as well as to enhance sustainable food production. Cover crops that are usually grown under CA to provide soil cover, may offer secondary benefits, depending on the farming system. The concept of growing cover crops is a relatively new phenomenon to smallholder farmers. Production of large biomass yields and weed suppression from cover crops were major challenges affecting success and uptake of CA technologies by smallholder irrigation farmers. Coupled with this, low soil fertility limit maize productivity and reduce water use efficiency on smallholder irrigation schemes in what is largely a water strained agro-ecology in South Africa. While cover cropping can increase maize productivity, benefits of different types of mulch are not well understood, leading to challenges in selecting the most appropriate cover crop species to grow in the Eastern Cape Province (EC) of South Africa (SA) which has a warm temperate climate. With respect to any new technology, smallholder farmers are more interested in the economic benefits. Cover crops have been defined as leguminous or non-leguminous plants used for ground cover in various temporal and special configurations used in crop or animal production systems. The purpose of these cover crops is to improve on or more of the following: soil erosion, availability and cycling of N, P, K, Ca and other nutrients, soil moisture and water infiltration, and weed or pest control (Eilitta et al., 2004).. Improvement of animal or human diet may be additional goals. This definition accommodates diverse systems which may include intercrop and sole-cropping systems. In the Eastern Cape Province of South Africa, a government initiative has promoted the growing of winter cover crops in smallholder irrigation schemes (Allwood, 2006). In other parts of Africa, legume food crops have been simultaneously grown with cereal staples to improve both soil cover and human diet (Eilitta et al., 2004). Winter experiments were undertaken in 2007 and 2008 to evaluate biomass accumulation, C and N uptake, weed suppression and response to fertilization. Winter cover crops planted included; oats (Avena sativa), grazing vetch (Vicia dasycarpa), faba bean (Vicia faba), forage peas (Pisum sativum) and lupin (Lupinus angustifolius). After cover crops were terminated, the effects of residues on weeds, fertility, moisture conservation and maize productivity were undertaken in the 2007/08 and 2008/09 summer seasons. Field studies were also done in the 2007/08 and 2008/09 summer seasons to investigate effects of strip intercropping patterns (3:2; 4:2; and 6:2 patterns) of maize (cv. PAN 6479) with mucuna (Mucuna pruriens) or sunnhemp (Crotalaria juncea) on maize productivity and summer cover crop biomass production. In a separate experiment effects of relay intercropping sunnhemp, mucuna and sorghum (Sorghum bicolor) on biomass accumulation and maize productivity were investigated. Decomposition, N and P release from both winter cover crops and summer cover crops were also assessed in laboratory incubation experiments. Oats, grazing vetch and forage peas cover crops produced mean dry mass of 13873 kg/ha, 8945.5 kg/ha and 11073 kg/ha, respectively, while lupin had the lowest dry mass of 1226 kg/ha over the two seasons. Oats responded to fertilization while, there was little or no response from the other winter cover crops. Oats and grazing vetch also reduced weed density by 90 % and 80 % respectively while lupin only reduced weed density by 23 % in relation to the control plots. Nitrogen uptake was 254 kg N/ha for oats while it was 346 kg N /ha for grazing vetch. In the subsequent summer season, grazing vetch and forage pea residues significantly (P < 0.01) improved soil inorganic N. Oat and grazing vetch residues significantly (P < 0.05) reduced weed dry masss and weed species diversity compared to plots with lupin residues and the control. Lack of maize fertilization tended to reduce maize yields but not for maize grown on grazing vetch residues. From an economic perspective, grazing vetch resulted in the highest returns. Decomposition of winter cover crops was much faster for grazing vetch followed by forage peas and lastly oats. Oats had 40 % ash free dry mass remaining after 124 days while grazing vetch and forage peas had 7 % and 16 % respectively. Maximum net mineralized N and P were greater for grazing vetch (84.8 mg N/kg; 3.6 mg P/kg) compared to forage peas (66.3 mg N/kg; 2.7 mg P/ha) and oats (13.7 mg N/kg; 2.8 mg P/kg). In the strip intercropping trials, sunnhemp achieved the highest biomass yield of 4576 kg/ha in the 3:2 pattern while mucuna achieved 1897 kg/ha for the same strip pattern. The 3:2 strip intercropping pattern slightly depressed yields, however, yield reduction was more pronounced in the first season where water stress was experienced. Growing maize on previous cover crop strips failed to increase maize productivity probably due to weed growth during the fallow reducing mineral N in these strips. Decomposition was faster in sunnhemp leaves and mucuna compared to sunnhemp stems. Sunnhemp stems had about 65 % of ash free dry mass remaining after the end of the experiment at 132 days while just over 10 % of mucuna and sunnhemp leaves still remained. Mucuna mineralized 60 mg N/kg and 3.2 mg P/kg and sunnhemp mineralized 45 mg N/kg and 3.5 mg P/kg. Relay intercropping did not significantly (P > 0.05) affect maize biomass and grain yield. Sorghum experienced the largest drop in biomass when relay-intercropped with maize. Mucuna resulted in the highest N uptake (271 kg N/ha) in sole cropping while sorghum had the lowest (88 kg N/ha). Grazing vetch results in high biomass yields with minimal fertilizer application in a warm-temperate climate. Grazing vetch mulch is also the most cost effective mulch for better early weed control, improving soil mineral N status, water conservation and ultimately enhanced maize productivity in smallholder irrigation maize-based systems. The 3:2 pattern maximizes summer cover crop biomass yields compared to the 6:2 and 4:2 patterns. However, the 3:2 pattern may slightly depress yields in a water stressed environment. Relay intercropping mucuna, sunnhemp and sorghum into a maize crop at 42 days after maize sowing has no effect on maize productivity while cover crop biomass yields are low. Having a long winter fallow period after maize harvesting, a common practice in the study area, reduces the positive impact of legume cover crops on soil mineral N. Results suggest that winter cover crops may result in weed control, soil fertility and maize yield improvement benefits while a long fallow period may cancel-out these benefits for summer cover crops. Grazing vetch is a cost effective cover crop that produces high maize yields with minimal fertilizer input. Maize growing on oat mulch requires more fertilizer application than crops growing on grazing vetch mulch. Conservation agriculture systems in which summer cover crops are grown alongside the maize crop with a long winter fallow period do not produce the intended CA benefits.
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- Date Issued: 2010
Evaluation and mapping of the spatial variability of soil fertility at Zanyokwe Irrigation Scheme in the Eastern Cape, South Africa
- Authors: Manyevere, Alen
- Date: 2010
- Subjects: Soil surveys -- South Africa -- Eastern Cape , Irrigation farming -- South Africa , Soil fertility -- South Africa -- Eastern Cape , Soils -- Analysis , Soils -- Testing , Crop yields , Soils Classification
- Language: English
- Type: Thesis , Masters , MSc Agric (Soil Science)
- Identifier: vital:11176 , http://hdl.handle.net/10353/d1001019 , Soil surveys -- South Africa -- Eastern Cape , Irrigation farming -- South Africa , Soil fertility -- South Africa -- Eastern Cape , Soils -- Analysis , Soils -- Testing , Crop yields , Soils Classification
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- Date Issued: 2010
Evaluation of antidiarrhoeal and toxicological properties of Hermannia Incana cav.: a South African medicinal plant
- Authors: Appidi, Jaipal Reddy
- Date: 2010
- Subjects: Sterculiaceae , Medicinal plants -- South Africa -- Eastern Cape , Diarrhea , Botany, Medical
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:11304 , http://hdl.handle.net/10353/259 , Sterculiaceae , Medicinal plants -- South Africa -- Eastern Cape , Diarrhea , Botany, Medical
- Description: Hermannia incana Cav. (Sterculiaceae), known as sweet yellow bells, is a medicinal plant used by the people of the Eastern Cape for the treatment of stomach-ache and diarrhoea. It has purgative and diaphoretic effects. It is a prostrate herb with yellow flowers and sparsely hairy and slightly glandular leaves, occurring in grassland and marshes in the Eastern Cape Province of South Africa. Based on the ethnomedical uses of this plant, the research project was designed to evaluate its antidiarrhoeal and toxicological properties. An ethnobotanical study of plants used for the treatment of diarrhoea in the Eastern Cape Province was carried out, using a questionnaire which was administered to herbalists, traditional healers and rural dwellers. This survey indicated a total of 17 plant species from 14 families. Elephantorrhiza elephantine (Burch.) Skeels, Hermannia incana Cav., Pelargonium reniforme Curt., Alepidea amatymbica Eckl. & Zeyh. and Bulbine latifolia (L.f.) Roem. et Schult. were the most frequently mentioned and highly recommended plants for the treatment of diarrhoea by both the traditional healers and rural dwellers. The root, bark and leaves are the common parts of plants used, while decoctions and infusions are the main methods of preparation. The agar dilution method was used to study the antimicrobial activity. The methanol extracts of the plant showed appreciable activity against Gram-positive and Gram-negative bacteria at concentrations ranging from 0.5 to 7.0 mg/ml. The acetone and water extracts of both the leaves and the roots showed moderate activity against Gram positive bacteria and less activity against Gram negative bacteria. All the extracts inhibited the growth of the fungi Aspergillus flavus, Aspergillus niger, and Mucor hiemalis with growth inhibition ranging from 54.31 percent to 96.67 percent at 0.1-10 mg/ml. None of the extracts suppressed the growth of Candida albicans at the maximum concentration (10 mg/ml) tested. iii In the in vivo antidiarrhoeal evaluation using Wistar rats, the aqueous extract at all the doses tested, significantly prolonged the time of induction of diarrhoea and also reduced the frequency of diarrhoeal episodes and fecal parameters (total number, number of wet, fresh and dry weight and water content of the faeces). The percentage inhibition of defecation and intestinal content (enteropooling) were increased in dose dependent manner. The doses also reduced the intestinal transit time of charcoal, masses and volumes of intestinal fluid (gastrointestinal motility). These results are indications of antidiarrhoeal property of H. incana leaf extract with the 600 mg/kg body weight of the extract being the most effective. In the toxicological evaluation using Wistar rats, the oral administration of the extract did not produce any significant effect on the liver and kidney body weight ratios, RBC, HB, PCV, MCV MCH, MCHC, RCDW, WBC, neutrophils, monocytes and basophils cholesterol, triacylglycerol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and atherogenic index. The extract also did not affect the levels of sodium, potassium, chloride, inorganic phosphorus, urea, creatinine, total protein, globulin, albumin, total and conjugated bilirubin. The activities of alkaline phosphatase, gamma glutamyl transferase and alanine aminotransaminase in the serum were increased by the extract whereas aspartate aminotransaminase was decreased. The levels of LUC, platelets, lymphocytes and eosinophils were significantly affected at 600 mg/kg body weight. The available evidence in this study suggests that the extract of H. incana leaf is mild, parameter and dose specific. The structure and distribution of foliar appendages on the leaves of this plant were investigated with the JEOL (JSM-6390LV) scanning electron microscope (SEM). Both glandular and non-glandular trichomes were observed. Long stalked glandular trichomes were present on both the abaxial and adaxial surfaces while short stalked glandular trichomes were present only on the adaxial surface. Glandular trichomes were capitate while nonglandular trichomes were stellate with many arms. Energy dispersive X-ray spectroscopyiv SEM showed that Al, Ca, K, Na, Ti and Si were the major constituents of the crystals analyzed from the leaf surfaces. The phytochemical screening of H. incana revealed the presence of bioactive antidiarrhoeal agents such as alkaloids, tannins, saponins, phenolics, triterpenes, cardiac glycosides, flavonoids, cardenolides and dienolides. Two flavonoids, epicatechin and 3, 5, 7, 2’ tetra-hydroxy flavone-3- O--D-glucopyranoside were isolated from the leaves of the plant through bio-active guided fractionation. Both these compounds were screened against diarrhoea causative organisms (Echerichia coli, Shigella flexneri, Bacillus cereus and Staphylococcus aureus) and exhibiting minimum inhibitory concentrations ranging from 12.5 to 100 μg/ml. The findings from this research have generally justified the traditional use of this plant for the treatment of diarrhoea in this province.
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- Date Issued: 2010