Isolation, propagation and rapid molecular detection of the Kalahari truffle, a mycorrhizal fungus occurring in South Africa
- Authors: Adeleke, Rasheed Adegbola
- Date: 2007 , 2013-04-03
- Subjects: Truffles -- Kalahari Desert , Fungi -- Identification , Mycorrhizal fungi -- South Africa , Edible fungi -- South Africa , Mushroom culture -- South Africa , Fungi -- Cultures and culture media -- South Africa , Truffles -- South Africa , Truffles -- Lifecycles , Mycorrhizal fungi -- Lifecycles
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3889 , http://hdl.handle.net/10962/d1002951 , Truffles -- Kalahari Desert , Fungi -- Identification , Mycorrhizal fungi -- South Africa , Edible fungi -- South Africa , Mushroom culture -- South Africa , Fungi -- Cultures and culture media -- South Africa , Truffles -- South Africa , Truffles -- Lifecycles , Mycorrhizal fungi -- Lifecycles
- Description: Terfezia pfeilii is an edible mycorrhizal fungus that thrives in the Kalahari Desert of southern Africa. It is best known by desert dwellers for its flavour and as a source of nutrition. Although the genus Terfezia is generally regarded as being an ectomycorrhizal mycobiont, the exact mycorrhizal type formed by T. pfeilli and its' associated host plants remains uncertain. Discovery of the host plants for T. pfeilii would first be required in order to further investigate the life cycle and cultivation of this truffle. This study focussed on the isolation of mycelia from the ascocarp, optimising the growth conditions of the mycelial cultures, rapid molecular identification of T. pfeilii, investigation of potential helper bacteria and mycorrhizal synthesis experiments. T. pfeilii ascocarps were harvested from the Spitskop Nature Reserve in Upington, South Africa. Ascocarps were successfully identified using both morphological and molecular methods. Despite the delayed growth mostly caused by contaminating microorganisms, the isolation of T. pfeilii mycelia culture was successful. Molecular techniques were used to confirm the identity of the pure culture. Further studies were conducted on ways to improve the growth conditions of the mycelial culture on Fontana medium. An optimum temperature of 32°C, the addition of Bovine Serum Albumin as a nitrogen source and a pH of 7.5 significantly improved the growth of T. pfeilii in vitro. A rapid PeR-based molecular method was developed to speed up the identification of T. pfeilii. Specific primers that can exclusively amplify the ITS region of T. pfeilii were designed and used to identify both the ascocarps and the mycelial culture. The specificity of these primers was confirmed by their inability to amplify DNA from the isolates of contamining fungi obtained during the isolation process. Molecular comparison was made to confirm the reclassification of South African samples of T. pfeilii as Kalaharituber pfeilii as proposed by Ferdman et al.,(2005). However, in this study, the name T. pfeilii has been retained. A total of 17 bacterial isolates were obtained from the fruiting bodies of T. pfeaii and these were tested for stimulation of mycelial growth in vitro, indole production and phosphate solubilising capabilities. Bacterial isolates that showed potential to be Mycorrhization Helper Bacteria (MHB) were identified as Paenibacillus sp., Bacillus sp. and Rhizobium tropici. Selected plant seedlings were inoculated with T. pfeilii cultures or ascocarp slurry in order to re-establish the mycorrhizal association. After 8 months, light microscopy observations revealed an endomycorrhizal type association between Cynodon dactylon and T. pfeilii. This was confirmed with molecular analysis using specific T. pfeilii ITS primers. After 15 months, molecular methods confirmed Acacia erioloba as another host plant. These results have provided essential information paving the way for further investigation into the life cycle and biology of the Kalahari truffle. , KMBT_363 , Adobe Acrobat 9.53 Paper Capture Plug-in
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- Date Issued: 2007
Catalytic activities of metallophthalocyanines towards detection and transformation of pollutants
- Authors: Agboola, Bolade Oyeyinka
- Date: 2007
- Subjects: Phthalocyanines Electrochemistry Pollutants -- Biodegradation Pollutants -- Measurement
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4427 , http://hdl.handle.net/10962/d1006922
- Description: Syntheses, spectral, electrochemical and spectroelectrochemical studies of new thiol-derivatised MPc complexes were satisfactorily carried out. For the first time, spectroelectrochemistry gave evidence for the formation of Ni²⁺/Ni⁺ process in a NiPc complex. Significant insights as to the nature of Fe⁺Pc and Ni⁺Pc spectra were obtained. Transformations of chlorophenols using chemical and photochemical methods are presented. For cobalt tetrasulphophthalocyanine catalysed oxidation of chlorophenols using hydrogen peroxide as the oxidant, types of oxidation products formed depended on the solvent conditions. Photolysis of aqueous solutions of chlorophenols in the presence of immobilised non-transition metal phthalocyanine photosensitisers onto Amerlite® was carried out. For the first time, MPcS[subscript mix] complexes were immobilised on Amberlite® for use in heterogeneous photocatalysis. Photolysis of the chlorophenols resulted mainly in the formation of chlorobenzoquinone derivatives. The generation of singlet oxygen (¹O₂) by these immobilised MPc photosensitisers was found to play a major role in their photoactivities. Modifications of gold electrodes with the newly synthesised thiol-derivatised MPc complexes via electropolymerisation and SAM techniques are presented. Cyclic voltammetry, impedance spectroscopy (NiPcs only) and spectroelectrochemical techniques (NiPcs only) confirmed that the complexes formed films on gold electrodes. Stable and well packed SAM films as evidenced by the voltammetric characterisation were obtained. For the first time, optimisation of the time for SAM formation based on CV technique was studied. First example of a formation of MnPc-SAM was achieved. Catalytic activities of the NiPc towards chlorophenol depended on the nature of the NiPc in the polymer films and also anti-fouling ability of the films depended on polymer film thickness. The FeTBMPc polymer modified gold electrode showed the best catalytic activity in terms of peak potential, E[subscript p] when compared to reported work in literature for nitrate electrooxidation. Cyclic voltammetry and spectroscopy studies showed that the CoPcs, FePcs and NiPcs catalysed nitrite oxidation involve 2 electrons in total while that of McPcs involve 1 electron. Better catalytic performance towards sulphite electrooxidation were obtained for the CoPcs, FePcs and MnPcs which have metal based redox processes within the range of the sulphite electrooxidation peak while the NiPcs which did not show metal based oxidation reaction performed less.
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- Date Issued: 2007
Implementation of learner-centred education by Grade 4 BETD in-service teachers in selected Namibia schools
- Authors: Amakali, Amram
- Date: 2007
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1849 , http://hdl.handle.net/10962/d1004457
- Description: Before independence Namibia's education system was predominantly teacher-centred. Soon after its independence Namibia embarked on education restructuring and transformation. A new education system, leamer-centred education, was introduced to cater for the needs of all Namibian learners. It was seen as an effective antidote to the stifling teacher-centred practices of the previous education system. A new programme, the Basic Education Teacher Diploma (BETD) was introduced to prepare teachers to teach in a leamer-centred approach. Research has, however, indicated that many Namibian teachers have a somewhat shallow or naIve understanding oflearner-centred education. This small-scale case study, conducted in Oshikoto region, focuses on two Grade 4 teachers. The study examined the two teachers ' perceptions and experiences oflearnercentred education and the teaching strategies they use to develop learners' understanding. The study uses a qualitative approach in its exploration of teachers ' lived experiences of becoming learner-centred. The data emerging from this case study identify a number of misconceptions and understandings of leamer-centred education and its implementation. The findings suggest that teachers ' misconceptions are caused by a lack of deep understanding about the epistemology and theory of learning which underpins Namibia's reform policies and principles.
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- Date Issued: 2007
An investigation of interpersonal relationships between management and lecturers in a College of Education in Namibia
- Authors: Amushigamo, Angelina Popyeni
- Date: 2007
- Subjects: Communication in education -- Namibia Teacher morale -- Namibia Employee motivation -- Namibia Teacher-administrator relationships -- Namibia College teachers -- Job satisfaction -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1850 , http://hdl.handle.net/10962/d1004458
- Description: Relationships are regarded as an important aspect of any organization's life. The purpose of this study was to investigate staff perceptions and experiences of interpersonal relationships between management and lecturers in a Namibian College of Education. I conducted a case study at the College where I teach. Two methods were used to collect data. Firstly, semi-structured interviews with two management members, two senior lecturers and two lecturers. Secondly, observation where practical aspects of interpersonal relationships in the College were observed. I used the interpretational data analysis technique to analyse my data. Themes and patterns were identified in the data, coded and sorted into categories. The study revealed staff unhappiness about the current situation in the College as far as communication is concerned. The College's hierarchical structure was described as top-down. As such, it does not allow for face to face communication. There is an absence of any social cohesion or sense of community. Relationships at a College level are characterized by personal conflict and difference. However, the study revealed a satisfaction with communication and relationships at a dl'partmentallevel. Five key features of interpersonal communication that are lacking in the College and that contribute to the unhealthy relationships in the College were identified. These are trust, respect, openness, feedback and the sharing of ideas and knowledge. Due to their absence, the College is divided into cliques. A strong desire for the establishment of interpersonal norms of openness, respect, honesty and trust was expressed. Participants expressed the need to establish an organization structure that allows for interaction with others in the College, flatter structures, teamwork and a collaborative cultu re. The study also emphasized participative democracy in building relationships. Participation in decision making is seen as satisfying the personal need to experience a sense of influence and achievement. There is evidence of a desire for distributed leadership where the College staff as a group of professionals lead the College collectively and collaboratively. There is a strong desire for a College where people are liked , valued, accepted by others and recognized for their efforts. Finally, Organization Development is recommended as an approach to enhance College staff relationships.
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- Date Issued: 2007
Investigating grade 10 geography teachers' implementation of a learner-centred approach in selected Namibian schools
- Authors: Angula, Adelheid
- Date: 2007
- Subjects: Geography -- Study and teaching (Secondary) -- Namibia Student-centered learning -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1851 , http://hdl.handle.net/10962/d1004459
- Description: Geography, more than other subjects in the curriculum, plays a central role in general education in equipping learners with skills and competencies needed for modern living and global citizenship. A learner-centred education in Namibia was adopted to provide more opportunities for learners to develop the required skills and competencies irrespective of their different cultural background. This small-scale case study investigated three Grade 10 geography teachers' understanding and implementation ofa learner-centred approach to gain insights into how leamer-centred education is being implemented in the context of Geography. The research design adopted a qualitative approach within an interpretative orientation. Data were collected through interviews, classroom observations and documentary analysis. The research was conducted at three selected schools in Oshikoto Region with three Grade 10 geography teachers The findings revealed, firstly, that teachers have a limited understanding of the key ideas ofLCE, such as, prior knowledge, role of questioning, and social interaction in learning; use of resources and the types of assessment activities which comply with the aims of LCE. Seconclly, the findings revealed that the policy documents, such as syllabuses, that are being used by the participants are not in line with the tenets ofLCE. Thirdly, teachers appeared to have limited subject knowledge, as revealed by their lack of understanding of how to translate the aims and assessment objectives into their daily lessons. The study therefore raises some possibilities for improving the implementation of LCE in the selected schools if the gaps as identified by this study are reduced.
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- Date Issued: 2007
The trouble with culture : Plato's critique of poetry
- Authors: Ansell, James D'Olier
- Date: 2007 , 2013-07-25
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2733 , http://hdl.handle.net/10962/d1004460
- Description: In this thesis I argue that Plato's critique of poetry, taken in its proper context, is a serious and relevant critique of popular culture. In the first chapter I argue that the poetic reforms proposed in Books 2 and 3 and Book 10 of Republic stand at the front of a total reform of Greek culture. I argue for the consistency of Plato's whole argument and then I claim that if we get Plato's targets right, not fine art or literature, and focus on appropriate modern analogues then we can see why his critique is still important. If we share his claim that we are influenced by popular culture in important and often insidious ways and agree that culture can promote corrupt values, then we have accepted the core of Plato's challenge. If we find his solution distasteful, then the task is to come up with a democratic alternative. In the remaining two chapters I focus specifically on the challenge to the poets, putting the other reforms to the side. In the second chapter I consider a possible reply to the challenge focusing on the worth of the poetry that was expelled. I first look elsewhere in the Platonic oeuvre at the account of beauty in Symposium and Phaedrus but I argue that neither of them gives anything like aesthetic value that could be usefully applied to poetry. Next I look to some modern accounts of aesthetic value. I argue that while they might go some of the distance against Plato's challenge, they face a difficult task because it is not sufficient positing the value, an account is needed of their positive benefit. In the third chapter I turn to a more direct response to the challenge. Arguably Aristotle offers such a response in the Poetics, in terms of the notion of katharsis. I consider two interpretative candidates for katharsis. The first takes the benefit of poetry to be psychological - katharsis is a purgation of otherwise pathological emotions. I argue that this fails because it misunderstands precisely what Plato's concerns about poetry are, and, furthermore, this account could even be compatible with Plato's worries. The second interpretation takes the benefit of poetry to be ethical- katharsis is a type of ethical clarification which is beneficial in training our emotional responses. I claim that the clarification, and education, is worryingly conventionalist, and doesn't take seriously that Plato's target was popular culture and not great, educative literature. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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- Date Issued: 2007
Address at the unveiling of the plaque in memory of Ruth First at the Ruth First residence, Aug 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University , First, Ruth, 1925-1982
- Language: English
- Type: text
- Identifier: vital:7656 , http://hdl.handle.net/10962/d1015784
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- Date Issued: 2007
Rhodes University postgraduates orientation welcome, 9 Feb 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7653 , http://hdl.handle.net/10962/d1015781
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- Date Issued: 2007
Vice Chancellor New staff welcome address, 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Type: text
- Identifier: vital:7646 , http://hdl.handle.net/10962/d1015774
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- Date Issued: 2007
Rhodes University 2007 Graduation Ceremonies Address
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: Text
- Identifier: vital:7628 , http://hdl.handle.net/10962/d1012595
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- Date Issued: 2007
Rhodes University : into 2007 and beyond
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7655 , http://hdl.handle.net/10962/d1015783
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- Date Issued: 2007
Preface to Rhodes University Research Report, 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7654 , http://hdl.handle.net/10962/d1015782
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- Date Issued: 2007
A preliminary perspective on the Office of the Vice-Chancellor and appeals related to exclusions and admissions
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7644 , http://hdl.handle.net/10962/d1015772
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- Date Issued: 2007
Rhodes University Orientation Week : Welcome address of the vice-chancellor of Rhodes University, Dr Saleem Badat, 5 February 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7651 , http://hdl.handle.net/10962/d1015779
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- Date Issued: 2007
Address at the opening of the OutRhodes Pride Week, 21 August 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7652 , http://hdl.handle.net/10962/d1015780
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- Date Issued: 2007
Vice Chancellor's message to the Toronto Old Rhodian Reunion
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7648 , http://hdl.handle.net/10962/d1015776
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- Date Issued: 2007
Message to the New Zealand Old Rhodian Reunion
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7647 , http://hdl.handle.net/10962/d1015775
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- Date Issued: 2007
Vice Chancellor's Oppidan Newspaper message, 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7649 , http://hdl.handle.net/10962/d1015777
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- Date Issued: 2007
Orientation Week : welcome message from the vice-chancellor Dr Saleem Badat, 2007
- Authors: Badat, Saleem
- Date: 2007
- Subjects: Rhodes University
- Language: English
- Type: text
- Identifier: vital:7650 , http://hdl.handle.net/10962/d1015778
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- Date Issued: 2007
SAIAB extension opening : lunch for the Deputy-Minister of Science and Technology
- Authors: Badat, Saleem
- Date: 2007
- Subjects: South African Institute of Aquatic Biodiversity (SAIAB)
- Language: English
- Type: text
- Identifier: vital:7657 , http://hdl.handle.net/10962/d1015785
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- Date Issued: 2007