Isolation, purification and effect of ligands on the nicotinic cholinergic receptor
- Authors: Kapp, Eugene Anthony
- Date: 1989
- Subjects: Ligands (Biochemistry) , Nicotinic receptors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4550 , http://hdl.handle.net/10962/d1018235
- Description: The nicotinic cholinergic receptor protein of the fish electric organ, Torpedo fuscomaculata, has been isolated, purified and shown to represent a true model for the nAChR from other species and higher vertebrates. It is an integral membrane protein composed of four different subunits, tightly associated with other functional, but non-specific proteins. Purification of the nicotinic cholinergic receptor by chromatofocusing demonstrates an improved method over that of affinity and ion-exchange chromatography. Gel chromatography and SDS-polyacrylamide gel electrophoresis show evidence of four subunits; a(40-44 kDa), 6(53 kDa ),'Y(63 kDa) and 6(66 kDa) despite some degradation of receptor molecules by intracellular proteases. Spectrophotometric and fluorimetric studies of receptor-ligand interactions, show the functional and chemical integrity of the receptor to remain intact after solubilisation. The effect of cholinergic ligands on purified receptor preparations indicate quenching of the intrinsic fluorescence of the receptor. Agonists, like acetylcholine, bind and cause local conformational transitions, changing the active region from a hydrophobic to a hydrophilic environment. This phenomenon is illustrated by the 10-fold increase in fluorescence when the receptor is in a desensitised state. Antagonists, such as d-Tubocurarine, block this conformational transition. In vitro rectus abdominis muscle preparations . show the nitrosamines, dimethylnitrosamine and diphenylnitrosamine, to be true agonists of the nAChR. However their low affinity and specificity for the receptor precludes them as photoaffmity labelling agents. Photoactivation of dimethylnitrosamine occurs when associated with an acidic hydrogen at the active site of the receptor, suggesting energy-transfer labelling to be more facile than photoaffmity labelling. The membrane-bound receptor, in the presence of these nitrosamines, undergoes conformational transitions regulating the opening and closing of the ion-channel. Desensitisation and receptor activation are shown to involve one and the same molecular transition.
- Full Text:
- Date Issued: 1989
It's a long way to go to heaven
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: English
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316175 , vital:59797 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD137-30
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Jazz d'O 89
- Authors: Unknown
- Subjects: McGregor, Chris--1936-1990 , Brotherhood of Breath (Musical group) , Shepp, Archie , Jazz
- Language: French
- Type: Article
- Identifier: vital:13716 , http://hdl.handle.net/10962/d1012728
- Description: Photocopied article from the French magazine Jazz Hot about the third edition of the festival Jazz d'O 89 in France. There is a picture of Philippe Gaillot with the article.
- Full Text:
Jean Dubuffet : a study of his concepts pertaining to the alienation of the artist
- Authors: Mather-Pike, Richard
- Date: 1989
- Language: English
- Type: Thesis , Masters , MFA
- Identifier: vital:21086 , http://hdl.handle.net/10962/6290
- Description: I decided on an investigation into the nature of Dubuffet's remarks concerning the plight of the artist in modern societies, believing that the difficulties facing the artist are, at the time of this writing, still in force, and perhaps even more so now than they were in his day. These difficulties I believe, arise in the form of curtailments on personal and aesthetic freedoms brought about as the result of the artist's position in, and conformity to, the norms and values of his society. The problem is I believe, that these value systems not only censor or curb artistic freedom but actually restrict all individual enterprises by demanding that the indi vidual conform to the greater wish of the group, at the expense of his own creative and individual potential.
- Full Text:
- Date Issued: 1989
Jesus is coming over and over
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: English
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316134 , vital:59792 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD137-25
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Ka dijo tsa gago tsa Moya
- Authors: Church Choir and Congregation , Composer not specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/313773 , vital:59526 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD132-23
- Description: Church hymn.
- Full Text: false
- Date Issued: 1989
Ka mponwane
- Authors: Oageng, Broer , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Botswana Mahalapye f-bs
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/313226 , vital:59466 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD131-07
- Description: Traditional Tswana song, accompanied by the Segapa Bow.
- Full Text: false
- Date Issued: 1989
Ka Tsholetsa
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316238 , vital:59804 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD137-37
- Description: Music Festival Performance with ululation accompaniment.
- Full Text: false
- Date Issued: 1989
Ke ipolela
- Authors: Music Workshop Group , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Botswana Kanamo f-bs
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/313247 , vital:59467 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD131-08
- Description: Practise and performance of church hymn.
- Full Text: false
- Date Issued: 1989
Ke lo naya taola entsha
- Authors: Church Choir and Congregation , Composer not specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/313746 , vital:59523 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD132-22
- Description: Church hymn.
- Full Text: false
- Date Issued: 1989
Ke lone lerato la Jesu
- Authors: Sacred Music Festival Participants , Composer not specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/314789 , vital:59639 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-14
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Ke Tsepile
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/315329 , vital:59702 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-66
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Kea Dumela
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/315496 , vital:59722 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-79
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Kea Dumela
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/316206 , vital:59800 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD137-33
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Kgosi lona ke mang
- Authors: Church Music Workshop Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Botswana Mahalapye f-bs
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/312920 , vital:59431 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD0130-01
- Description: Traditional Tswana song, accompanied by drum.
- Full Text: false
- Date Issued: 1989
Khumbula Ukwenza Isabatha
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/315000 , vital:59666 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-36
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Koma
- Authors: Oageng, Broer , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa Botswana Mahalapye f-bs
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/313071 , vital:59448 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD0130-13
- Description: Traditional Tswana song, accompanied by the Segapa Bow.
- Full Text: false
- Date Issued: 1989
Konyana Ya Modimo
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: English
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/315112 , vital:59678 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-48
- Description: Music Festival Performance with clapping accompaniment.
- Full Text: false
- Date Issued: 1989
Konyana Ya Modimo
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa City not specified f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/315512 , vital:59725 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-81
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989
Konyana ya Modimo
- Authors: Sacred Music Festival Participants , Composer Not Specified , Dargie, Dave
- Date: 1989
- Subjects: Folk music , Field recordings , Africa, Sub-Saharan , Africa South Africa Ga-Rankuwa f-sa
- Language: Setswana
- Type: sound recordings , field recordings , sound recording-musical
- Identifier: http://hdl.handle.net/10962/315140 , vital:59682 , International Library of African Music, Rhodes University, Makhanda, South Africa , Dave Dargie Field Tapes, Rhodes University, Makhanda, South Africa , DD136-52
- Description: Unaccompanied Music Festival Performance.
- Full Text: false
- Date Issued: 1989