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Evaluation of the safety and efficacy of topical mometasone furoate formulations

  • Authors: Chamboko, Bernadett Vongayi
  • Date: 2007
  • Subjects: Adrenocortical hormones -- Physiological effect , Drugs -- Testing , Dermatopharmacology , High performance liquid chromatography
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:3748 , http://hdl.handle.net/10962/d1003226 , Adrenocortical hormones -- Physiological effect , Drugs -- Testing , Dermatopharmacology , High performance liquid chromatography
  • Description: The human skin blanching assay (HSBA) is a well-researched and validated method for the bioequivalence assessment of topical corticosteroids. Traditionally, visual assessment of skin blanching has been used. Such testing methods are not conducive for interlaboratory comparisons. Regulatory bodies prefer less subjective methods of analysis. The FDA released guidelines on the assessment of bioequivalence for topical corticosteroids that recommends the use of a chromameter as a reliable method to measure skin blanching although the use of visual assessment with acceptable validation is also provided for. However, the FDA does not elucidate on the manipulation and handling of the chromameter during skin blanching measurements. The purpose of this project was several fold, which included investigations to standardize the manipulation and handling of a chromameter. In particular, measures to avoid skin whitening resulting from the effects of pressure on the skin during chromameter use were investigated. Other methods of analysis should surpass or at least be comparable to the HSBA if such methods are to be used for the assessment of topical corticosteroids. Microdialysis is a relatively new technique for assessing the rate at which drug penetrates the skin. The advantage of using this method is that there are fewer restrictions for selection of an appropriate study population unlike those required for the HSBA where one has to be both a ‘responder’ and a ‘detector’ for their results to be used in data analysis. Microdialysis was investigated by initially conducting experiments in which microdialysis probes were embedded into topical formulations containing mometasone furoate (MF) and the initial results revealed that relatively low drug was released from the formulations. These results indicated that should microdialysis be applied to measure the in vivo release of MF from such topical formulations following application to the skin, even lower concentrations of MF would likely result in the dialysate, necessitating the need for ultra-high sensitive methods of analysis. Typically, the availability of an appropriate analytical technique such as liquid chromatography coupled with mass spectrometry (LCMS) would be a pre-requisite for such in vivo studies. However, only high-pressure liquid chromatography (HPLC) and other less sensitive equipment was available in the laboratories. The study objectives were therefore focussed on in vitro assessment of the release of MF from topical formulations using microdialysis and Franz cells. In addition, the in vivo release of MF was also studied using the HSBA. Data obtained from the microdialysis experiments were compared with the data obtained from the Franz cell diffusion studies in order to provide information on the pharmaceutical availability of MF from the various topical MF dosage forms. Subsequently, pharmaceutical equivalence was investigated from the comparative pharmaceutical availability data using statistical analysis. An additional objective was to attempt to correlate in vitro with in vivo data (IVIVC) to establish a model that could be used to assess safety and efficacy of generic topical drug products. The in vivo data obtained from the HSBA were processed according to the FDA requirements and these pharmacodynamic data were subsequently compared with the microdialysis and Franz cell results. In summary the objectives of this project were: 1. To develop a system to improve the reproducibility of the use of a Minolta® chromameter and compare this with the standard/normal manipulation and handling of such instruments. 2. To develop and validate an HPLC method for the analysis of MF for use with in vitro diffusion studies using microdialysis and Franz cells. 3. To conduct a comparative HSBA on proprietary MF topical creams from two different countries in accordance with the FDA guidance. 4. To assess the pharmaceutical equivalence of topical formulations containing MF using Franz diffusion cells and in vitro microdialysis. 5. To compare the in vivo data obtained from the HSBA with those obtained in vitro using microdialysis and Franz cells.
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  • Date Issued: 2007

The value of a Rhodes University degree and securing employment

The conflict between adaptation and constraint : the case of the Siphonariid limpets

  • Authors: Chambers, Richard Jonathan
  • Date: 1995
  • Subjects: Siphonaria -- Adaptation Limpets -- Adaptation Pulmonata
  • Language: English
  • Type: Thesis , Doctoral , PhD
  • Identifier: vital:5657 , http://hdl.handle.net/10962/d1005340
  • Description: The reproductive strategies of marine invertebrates have been related to various aspects of both their ecology, and their phylogenetic history. It has been the purpose of this thesis to try and separate these components among Siphonaria, a group of marine pulmonates. The taxonomy of these species is revised and I conclude that nine species are valid. All species deposit benthic egg masses and development may be either direct (S. anneae, S. compressa, S. dayi, S. nigerrima, S. serrata and S. tenuicostulata) or planktonic (S. capensis, S.concinna and S. oculus). Data on distribution and life-history relating to mode of larval development is then presented for 26 species of Siphonaria worldwide. Fifteen species are direct developers, nine are planktonic developers and a further two appear to have a dual developmental capacity, retaining both the velar swimming apparatus of a planktonic developer and the crawling foot of a direct developer. Direct developing species hatch from larger egg capsules, and generally occur higher on the shore than planktonic developers. Worldwide, planktonic developers are more widespread than direct developers, and individual planktonic species may have a greater latitudinal range. In most S~honaria subgenera, mode of larval development appears to be constant, although two subgenera (Patel/opsis and Sacculosiphonaria) include both developmental types. Locally, the intertidal zonation of three sympatric species (S. capensis, S. concinna and S. serrata) does not support a model which predicts direct development on the high shore and planktonic development on the low shore. However, distributions do correspond to particular intertidal microhabitats, and while there may be no, direct relationship between mode of larval development and intertidal height, the physical structure of egg masses, and the microhabitats used for spawning appear adaptive with regards to desiccation in the intertidal. S. concinna (planktonic development) and S. serrata (direct development) occur in similar microhabitats and are likely to be under similar selection pressures. In having different modes of larval development, there appears to be more than one optimal solution in a particular selective regime. In addition, both species seem to apportion similar amounts of energy to reproduction for each spawning episode, and also annually suggesting an optimum allocation of resources to reproduction. Genetic investigations using PolyAcrylamide Gel Electrophoresis (PAGE) confirm the status of the southern African species initially described, and indicate greater genetic variability associated with planktonic developing species than direct developing species. The systematic relationships revealed by DNA fingerprinting support the current classification systems, and also have implications with regards the evolution of larval development: direct development may be the plesiomorphic condition in, and among, some Siphonaria groups. There are both phylogenetic and adaptive explanations for the distribution of reproductive mode among benthic marine invertebrates. An evolutionary question, however, is not just a matter of either adaptation or constraint, it is a combination of these. Both contribute to the distribution of developmental mode among Siphonaria.
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  • Date Issued: 1995

Production of Cydia pomonella granulovirus (CpGV) in a heteralogous host, Thaumatotibia Leucotreta (Meyrick) (False codling moth)

  • Authors: Chambers, Craig Brian
  • Date: 2015
  • Subjects: Cryptophlebia leucotreta -- South Africa , Codling moth -- South Africa , Apples -- Diseases and pests -- South Africa , Codling moth -- Biological control -- South Africa , Insect pests -- Biological control -- South Africa , Biological pest control agents -- South Africa , Baculoviruses -- South Africa
  • Language: English
  • Type: Thesis , Doctoral , PhD
  • Identifier: vital:5935 , http://hdl.handle.net/10962/d1017906
  • Description: Cydia pomonella (Linnaeus) (Family: Tortricidae), the codling moth, is considered one of the most significant pests of apples and pears worldwide, causing up to 80% crop loss in orchards if no control measures are applied. Cydia pomonella is oligophagous feeding on a number of alternate hosts including quince, walnuts, apricots, peaches, plums and nectarines. Historically the control of this pest has been achieved with the use of various chemical control strategies which have maintained pest levels below the economic threshold at a relatively low cost to the grower. However, there are serious concerns surrounding the use of chemical insecticides including the development of resistance in insect populations, the banning of various insecticides, regulations for lowering of the maximum residue level and employee and consumer safety. For this reason, alternate measures of control are slowly being adopted by growers such as mating disruption, cultural methods and the use of baculovirus biopesticides as part of integrated pest management programmes. The reluctance of growers to accept baculovirus or other biological control products in the past has been due to questionable product quality and inconsistencies in their field performance. Moreover, the development and application of biological control products is more costly than the use of chemical alternatives. Baculoviruses are arthropod specific viruses that are highly virulent to a number of lepidopteran species. Due to the virulence and host specificity of baculoviruses, Cydia pomonella granulovirus has been extensively and successfully used as part of integrated pest management systems for the control of C. pomonella in Europe and around the world, including South Africa. Commercial formulations have been typically based on the Mexican strain of CpGV. However due to long-term multiple applications of CpGV and the reliance on CpGV in organic farming practices in Europe, resistance to the CpGV-M strain has developed in a number of field populations of C. pomonella. This study aimed to identify and characterize novel isolates of CpGV in South Africa and compare their virulence with the commercial standard CpGV-M. Secondly, since C. pomonella is difficult to culture on a large scale, an alternate method of CpGV production was investigated in order to determine if CpGV could be produced more efficiently and at a reduced cost without negatively impacting the quality of the product. Several isolates of CpGV were recovered either from field collected larvae or from a laboratory-reared C. pomonella colony. Characterisation of DNA profiles using a variety of restriction enzymes revealed that only a single isolate, CpGV-SA, was genetically different from the Mexican strain of the virus used in the commercially available CpGV based products in South Africa. In dose-response bioassays using CpGV-SA, LC₅₀ and LC₉₀ values for neonate C. pomonella larvae were 3.18 x 10³ OBs/ml and 7.33 x 10⁴ respectively. A comparison of these values with those of CpGV-M indicated no significant difference in the virulence of the two isolates under laboratory conditions. This is a first report of a genetically distinct CpGV isolate in South Africa. The biological activity and novelty of CpGV-SA makes this isolate a potentially important tool for CpGV resistance management in South Africa. In order to justify production of CpGV in an alternative host, studies on the comparative biological performance of C. pomonella and T. leucotreta based on oviposition, time to hatch, larval developmental times and rearing efficiency as well as production costs were performed. Thaumatotibia leucotreta was found to be more fecund and to have significantly shorter egg and larval developmental times. In addition, larval production per unit of artificial diet was significantly higher than for C. pomonella. This resulted in T. leucotreta being more cost effective to produce with implications for reduced insectary space, sanitation practices as well as the labour component of production. Virus yield data generated by inoculation both C. pomonella and T. leucotreta with nine concentrations of CpGV resulted in comparable virus yields, justifying the continuation of the research into production of CpGV in T. leucotreta. It was important to determine the LC and LT values required for mass production of CpGV in late instar T. leucotreta larvae. Dose- and time-response bioassays with CpGV-M were conducted on artificial diet to determine these values. Fourth instar LC₅₀ and LC₉₀ values were 5.96 x 10³ OBs/ml and 1.64 x 10⁵ OBs/ml respectively. LT50 and LT90 values were 81.10 hours and 88.58 hours respectively. Fifth instar LC₅₀ and LC₉₀ values were 6.88 x 10⁴ OBs/ml and 9.78 x 10⁶ OBs/ml respectively. LT₅₀ and LT₉₀ values were 111.56 hours and 137.57 hours respectively. Virus produced in fourth instar T. leucotreta larvae was bioassayed against C. pomonella neonate larvae and compared to CpGV-M to establish if production in the heterologous host negatively affected the virulence of the isolate. No significant difference in virulence was observed between virus produced in T. leucotreta and that produced in C. pomonella. The data generated in the bioassays was used in CpGV mass production trials to evaluate production. All production methods tested produced acceptable virus yields. To examine the quality of the virus product, genomic DNA was extracted from larval cadavers and subjected to REN analysis with HindIII. The resulting DNA profiles indicated that the virus product was contaminated with the homologous virus, CrleGV. Based on the above results, the use of T. leucotreta as an alternate host for the in vivo production of CpGV on a commercial basis is not at this stage viable and requires further investigation before this production methodology can be reliable used to produce CpGV. However, this study has shown that CpGV can be produced in a homologous host, T. leucotreta and significant strides have been made towards developing a set of quality control standards that are essential for further development of successful production methodology. Finally a novel isolate of CpGV has been identified with comparable virulence to the CpGV-M. This is an important finding as it has broad reaching implications for resistance management of CpGV products in South Africa.
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  • Date Issued: 2015

Stakeholder relationship management and the perception of trust: a case study of a salient stakeholder

  • Authors: Chambers, Craig Brian
  • Date: 2022-06
  • Subjects: Stakeholder management South Africa Sundays River (Eastern Cape) , Stakeholder theory , Trust , Industrial priorities , Social capital (Sociology)
  • Language: English
  • Type: Academic theses , Master's theses , text
  • Identifier: http://hdl.handle.net/10962/419060 , vital:71612
  • Description: Trust is an essential aspect in developing meaningful relationships between firms and their stakeholders. Mayer, Davis and Schoorman (1995, p.709) define trust as “the willingness of a party to be vulnerable to the actions of another party based on the expectation that the other party will perform a particular action important to the trustor irrespective of the ability to monitor or control that other party”. This study took the form of an explanatory case study that focussed on an interaction between a firm and a salient stakeholder and attempted to explain how this interaction influenced the perceived trustworthiness of the firm in stakeholders that were observing the interaction. The study attempted to add to the work of Crane (2020), whose research had focussed on stakeholder connectedness, and specifically how this could be utilized by firms interacting with stakeholders to engender trust in a wider group of stakeholders. The research aimed to evaluate how the Sterile Insect Release programme, operating by the firm in the Sunday’s River Valley, was given a second chance through engagement with a salient stakeholder and how the interaction was observed by a wider group of stakeholders leading to perceived stakeholder trustworthiness and social capital. The study had five objectives, namely to; (1) analyse how a firm’s ability, benevolence, and integrity enables (or does not engender) the development of the firm’s trustworthiness amongst its stakeholders; (2) analyse how the firm’s engagement with a salient stakeholder enables (or does not engender) increased levels of trustworthiness in the firm by the stakeholders that are observing its interaction with a salient stakeholder; (3) analyse the benefits of social capital gained through increased levels of stakeholder trust; (4) identify good practice guidelines for firm engagement with salient stakeholders to ensure the correct cues are portrayed to observing stakeholders for the development of the trustworthiness of the firm; and (5) make recommendations related to management practices that will support the development of trust. The research adopted a qualitative approach and data were collected through semi-structured interviews with three groups of stakeholders that were present at the meeting. A review of the literature was undertaken to identify various propositions for the research and from which a theory-driven coding memo was developed. The derived propositions were as follows: (1) observing a firm’s engagement with a single salient stakeholder will provide cues to observing stakeholders about the trustworthiness of the firm, and (2) perceptions of a firm’s trustworthiness will result in increased social capital between the firm and its stakeholders. The first proposition was divided into three sub-propositions for the study which stated; (a) observing a firm’s engagement with a single salient stakeholder will provide cues to observing stakeholders about the ability of the firm; (b) observing a firm’s engagement with a single salient stakeholder will provide cues to observing stakeholders about the benevolence of the firm; and (c) observing a firm’s engagement with a single salient stakeholder will provide cues to observing stakeholders about the integrity of the firm. From the literature analysis, various themes were identified which include, observed interactions, ability, benevolence, integrity, trustworthiness, and increased social capital. The research findings confirmed the propositions as being relevant to the case study. The findings confirmed that ability, benevolence, and integrity are key attributes in portraying trustworthiness when interacting with a salient stakeholder. All three attributes were noted by the interviewees as being important to observing stakeholders in the development of trust in a firm, although ability and integrity were found to be the dominant attributes in this case study. The findings did present some evidence that an increased level of perceived trustworthiness in the firm did positively influence the levels of social capital, however, this was not a common theme among all the interviewees. In the light of these findings, it is recommended that firms utilize stakeholder models to identify salient stakeholders that are influential within the markets in which they operate. The firm should actively pursue a strong relationship with these stakeholders, and further attempt to generate opportunities to interact with these stakeholders in a public forum where stakeholder connectedness could be utilized to develop perceived trust in the wider group of stakeholders. The firm should focus on open, honest, and transparent communication which are key aspects for portraying the attributes of ability, benevolence, and integrity. The study addresses a gap in the trust literature at a stakeholder level and therefore has contributed to the trust literature by addressing how the connectedness between stakeholders can lead to trust in a wider group of stakeholders by increasing efficiency during these interactions and utilizing situational circumstances to build social capital. , Thesis (MBA) -- Faculty of Commerce, Rhodes Business School, 2023
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  • Date Issued: 2022-06

Reason and eros

  • Authors: Chalmers, W D
  • Date: 1967 , 2014-06-09
  • Subjects: Philosophy , Love , Reasoning
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:2748 , http://hdl.handle.net/10962/d1013331
  • Description: This study is not intended as a work of research into any existing body of philosopny. It is, rather, an independent inquiry into the origins and the objective of philosophical activity. In this it assumes the somewhat enigmatic role of a philosophy of philosophy.
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  • Date Issued: 1967

Systematic marine spatial planning and monitoring in a data poor environment: a case study of Algoa Bay, South Africa

  • Authors: Chalmers, Russell
  • Date: 2012
  • Subjects: Spatial ecology -- South Africa -- Algoa Bay , Coastal zone management -- South Africa -- Algoa Bay
  • Language: English
  • Type: Thesis , Doctoral , PhD
  • Identifier: vital:5373 , http://hdl.handle.net/10962/d1015695
  • Description: Globally the failure of traditional fisheries management approaches is evident through the increasing number of overexploited or depleted marine stocks. Past sectoral management has failed to address cumulative impacts of fisheries activities on ecosystem health. Ecosystem based approaches have been advocated as a viable alternative for sustainable management of marine ecosystems as they present a holistic and precautionary approach, which integrates management of multiple activities with that of maintaining ecological health. Although conceptually advanced, implementation has been poor due to the complexities of competing ecological and socio-economic management objectives. Marine spatial planning can facilitate the implementation of ecosystem based management as it is able to address the spatial heterogeneity of biological communities and anthropogenic activities. Ecosystem based management approaches aim to address the full range of anthropogenic drivers on the marine environment, including but not limited to fisheries, tourism, coastal development, and land and marine based pollution sources amongst others. Fisheries activities have a direct impact on the local marine environment and were therefore the focus of this study which forms a starting point for implementing ecosystem based management in Algoa Bay. It is envisaged that future research will build on this foundation and include additional anthropogenic drivers into the management and monitoring strategies developed in this study in order to achieve a truly holistic ecosystem approach to management in Algoa Bay. Algoa Bay is situated centrally within the warm-temperate Agulhas bioregion along the east coast of South Africa and is the largest and best formed logarithmic spiral bay along this section of coastline. A large city, two commercial ports and several coastal settlements are located within Algoa Bay and a wide range of marine based activities occur within the area. A large section of the coastline is proclaimed as a National Park yet only two small offshore marine areas are formally protected. The development of a large marine protected area (MPA) adjoining the terrestrial section was proposed in the mid-1990s but a lack of adequate spatial data with which to quantify the fishery costs and conservation benefits led to wide scale public opposition and halted the declaration process. The primary goal of this study was to obtain and analyse baseline data to understand spatial and temporal trends in the distribution and abundance of fish populations and fisheries activities in order to develop a spatial framework for marine conservation and management in a data limited situation using Algoa Bay, South Africa as a case study. Furthermore, it aims to contribute to the development of a monitoring framework to evaluate the success of implementation and the resultant changes in biological and socio-economic environments. This information will be used to re-initiate the stakeholder engagement process in the future.
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  • Date Issued: 2012

An investigation into the feeding biology and factors influencing the population dynamics of Terebrasabella heterouncinata (Polychaeta: Sabellidae), a problematic tube-dwelling polychaete in farmed abalone in South Africa

  • Authors: Chalmers, Russell
  • Date: 2003
  • Subjects: Polychaeta -- South Africa , Sabellidae -- South Africa , Abalones -- Feeding and feeds -- South Africa
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5291 , http://hdl.handle.net/10962/d1005136 , Polychaeta -- South Africa , Sabellidae -- South Africa , Abalones -- Feeding and feeds -- South Africa
  • Description: Since the discovery of a new species of sabellid worm in 1993, the Californian and South African abalone industries have experienced increasing shell breakage and reduced growth rates of abalone, resulting in reduced market value of product. This polychaete worm can reach very high infestation levels. It was described as Terebrasabella heterouncinata in 1999, having originated from the South African coastline where it had been observed on several wild mollusc species. Although not a shell borer, it occupies tubes on the growing edge which are formed by deposition of nacreous shell over sabellid larvae by the host abalone. Heavy sabellid infestation levels have placed the economic viability of several South African farms under threat. Past research methods have not been successful in establishing effective means of eradication or control, partly due to the lack of knowledge of basic biological information on this species. This study forms the first part of an ongoing research project into the biology of terebrasabella heterouncinata. Abalone farms in South African utilise two main diets in abalone culture. An artificial formulated feed, Abfeed, is produced in South Africa and is used by the majority of farms as it increases abalone growth rate. Kelp, a natural diet of abalone, is harvested and also used as a feed. Farmers have observed higher sabellid infestation levels on Abfeed-fed abalone and suspect that diet may be an important factor influencing sabellid infestation. Abalone stocking density has also been identified as a potential factor influencing sabellid population growth on commercial farms. In order to better understand sabellid nutrition, the feeding behaviour of the sabellid in response to suspended solids of various particle size classes from both abalone diets were investigated. Both the effect of abalone diet and stocking density and their effects on sabellid morphometrics were quantified. The proximate composition and particle size distribution of suspended-solids present within kelp and Abfeed abalone raceways were also quantified. Results indicate that sabellid feeding follows no structured behavioural pattern for both abalone diets. The quantity of suspended solids in kelp raceways was greater than in Abfeed raceways (p<0.02) while there was no difference in particulate level between three abalone stocking densities (p>0.05). Protein (p<0.0001) and energy (p<0.0.1) levels of suspended solids were significantly greater in raceways dedicated to feeding Abfeed. The infestation level (number of tubes/centimetre on the grwoing edge) was significantly higher (p<0.001) in Abfeed-fed abalone. The morphometrics of the sabellids indicated that sabellids from Abfeed-fed abalone were larger in various body measurements: length (p<0.00001); neck width (p<0.001); base width (p<0.001); and surface area (p<0.001). This study suggests that abalone diet has an important influence on the infestation level and size of sabellids and that this is likely to be due to the fragmentation and leaching of nutrients from the artificially enriched commercial abalone diet.
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  • Date Issued: 2003

The monitor and synchroniser concepts in the programming language CLANG

  • Authors: Chalmers, Alan Gordon
  • Date: 1985
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4616 , http://hdl.handle.net/10962/d1006132
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  • Date Issued: 1985

Children in conflict with the law: assessing the implementation of the Child Justice Act 75 of 2008 in the Eastern Cape province

  • Authors: Chakuwamba, Kapesi Antony
  • Date: 2015-06
  • Subjects: South Africa. -- Child Justice Act, 2008 , Children -- Legal status, laws, etc
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10353/24945 , vital:63683
  • Description: The study explored efforts made by various stakeholders involved in the implementation of the Child Justice Act (CJA). It is essential to get insight on the roles of stakeholders in the child justice and view how their efforts could or do not warrant an effective coordination of the child justice system. This study was inductive in nature. Primary data came from in-depth interviews with individuals and government officials as well as focus groups with children in conflict with the law. Various assumptions relating to delinquent behaviour were highlighted by a description of the various factors underlying or contributing to this behaviour. The theories discussed such as social disorganization and differential association are relevant to this study and help to understand juvenile delinquency. It is essential to look at the extent and causes of juvenile delinquency as well as the history of child justice in South Africa, so that this can be used as a yardstick to measure the development of the law on child justice and see if the coming of the new CJA has made improvements to address juvenile delinquency. This study showed that the development of international and regional norms and standards on juvenile justice such as the UN Convention on the Rights of a Child, the UN Minimum Rules for Administration of Juvenile Justice, the UN Standard Minimum Rules For the Protection of Juveniles Deprived of their Liberty the UN Guidelines for Prevention of Juvenile Delinquency and the African Charter on the Rights and Welfare of the Child provide a comprehensive framework at the international level within which the issue of child justice should be understood. The study also argued that although the CJA brought new innovations in the criminal justice system, challenges in various key provisions of CJA such as assessment, preliminary inquiry, diversion, sentencing, child and youth care centres are noted. Other key findings include lack of training or capacity building, unavailability of budget and public education awareness. There is a need to look at these challenges to improve the multi-sectoral implementation of the CJA. , Thesis (LLM) -- Faculty of Law, 2015
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  • Date Issued: 2015-06

Housing delivery and empowerment in Post - Apartheid South Africa: The Case of Nkonkobe Municipality

  • Authors: Chakuwamba, Kapesi Antony
  • Date: 2009-12
  • Subjects: Housing -- Development , Nation-Building , Democratization
  • Language: English
  • Type: Master's theses , text
  • Identifier: http://hdl.handle.net/10353/23293 , vital:57027
  • Description: The core argument in the study is that there have been little prospects of housing delivery in Nkonkobe Municipality. The obstacles for housing development in this area are embedded in the inflexibility of current policies and legacies of the colonial as well as the Apartheid era. Furthermore, the findings indicate that local as well as external sociopolitical practices such as lack of funding, unavailability of land, corruption, politicking, lack of community participation, impact of macro-economic policy, planning and lack of capacity seem to hamper the process of housing delivery. Hence, there is a need for an alternative development strategy which is adopted as the conceptual framework of this study. This is to enhance housing development in most rural areas. This conceptual framework propounds that housing provision in Nkonkobe region should be enhanced in the context of empowerment, nation-building and democratization in the post-Apartheid South Africa. The problem of housing has become a worldwide phenomenon. Compared to other countries, the number of people in need of adequate shelter has remarkably increased and in South Africa the number of people who live in substandard housing and those in desperate need of shelter is enormous. However, in the study a view of housing provision in post-Apartheid South Africa is advocated through looking at the nature of the state and its capacity to deliver quality housing service. A qualitative methodology comprising in-depth interviews and observation was employed in order to gain a deeper understanding of the effectiveness of the municipality in delivering quality service to its people. The ideological perspectives view historical causes and present day reasons for the perpetuation of the housing problem from different angles. A strategy based on alternative development is proposed in this study. The conceptual framework views the poor as active people engaged in the production of their own lives not passive recipients of state development projects. An underlying assumption is that housing provision is influenced by previous state forms and spatial practices. Therefore, the level of community participation could influence the success of housing development projects. , Thesis (MA) -- Faculty of Social Sciences and Humanities, 2009
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  • Date Issued: 2009-12

A critical analysis of the dynamics of intergovernmental relations in Zimbabwe

  • Authors: Chakunda, Vincent
  • Date: 2017
  • Subjects: Intergovernmental fiscal relations -- Zimbabwe International relations
  • Language: English
  • Type: Thesis , Doctoral , Public Administration
  • Identifier: http://hdl.handle.net/10353/7979 , vital:31345
  • Description: The field of intergovernmental relations (IGR), both from a conceptual and practical perspective presents a contested order in Zimbabwe’s political and public administration discourse, with a fairly long and complex historical and institutional context. The advent of colonialism in Zimbabwe (then Rhodesia) gave birth to a dualised form of government with a separatist development agenda. The dualistic model of government was anchored on a segregationist centralist ideology that advanced a white supremacist agenda while entrenching underdevelopment in native areas. This was attained through the use of draconic and ingrained racially discriminatory laws, ordinances and policy enactments, inter alia, the 1910 High Commissioner’s Proclamation, the Native Councils Act, the African Councils Act, and the District Councils Act which supported the overriding philosophy of colonialist hegemony. The direct rule policy was used and in practice, administrative, political, judicial and legislative powers were under the purview of the whites. Under this political dispensation, the nature of IGR was typically a master-servant relationship as African institutions had limited policy latitude under the tentacles of race-driven white control over the socio-economic and political space with an exploitative and subservient underpinning. The demise of colonialism and the birth of independent Zimbabwe in 1980 ushered a new political dispensation. The post-independence government embarked on a number of reforms aimed at dismantling the racist undertones of government. These reforms include the expanded decentralization frameworks supported by legislative instruments and policies such as the 1984 Prime Minister’s Directive and the 1996 thirteen principles of decentralization. Other key reforms are the 1996 Urban Councils Act, Chapter 29:15 and the 1988 Rural District Councils Act, Chapter 29:13. However, it is important to note that despite this plethora of legislation and reforms purportedly meant to dismantle racist backed institutional differentiation, the new national government did not depose its excessive control on sub-national governments. It is therefore an insoluble contradiction that the legislation and institutions created in post-independence Zimbabwe promoted the autonomy of sub-national governments while broadening democracy and citizen participation. This era rather presents an aporetic discourse epitomized by the national government’s perfection of colonial dominance approaches through creating legislation and institutions to retain wide and extensive control of sub-national governments. The Global Political Agreement of 2009 culminated into the promulgation of the Constitution of Zimbabwe Amendment Number 20 of 2013 with provisions for devolution of power, Provincial and Metropolitan Councils and the enshrinement of Local Government as a tier of government with constitutionally guaranteed autonomy. However, despite these reforms with far reaching implications on the configuration of IGR, the ZANUPF led national government is lethargic in implementing them. This has sparked controversy as these constitutional reforms and their potential to promote an integrated and efficient system of governance may turn out to be a pyrrhic victory. This is so because the necessary legislation anchoring the constitutional reforms may not be created in the foreseeable future as the current national government views devolution, for instance, as tantamount to surrendering political power to sub-national institutions. The purpose of the study was to critically examine the dynamics of intergovernmental relations in Zimbabwe. The overlapping authority model of IGR and the theory of networked governance underpinned the study. A comparative study of IGR was conducted focusing on two federal nations (United States of America) and Nigeria) and two unitary nations (United Kingdom and South Africa). A qualitative phenomenological methodology was used and the sample size was 20 respondents selected using the purposive sampling technique. Data was collected using in-depth interviews and analyzed using thematic analysis and critical discourse analysis. Key findings of the study reflected on the conception and relevance of IGR in unitary nations in contrast to classical perspectives that restricted the field as a discourse of federalism. The study established that the unitary system of Zimbabwe is anchored on a strong centralist ideology that suffocates the autonomy of sub-national institutions. In the same context, there is absence of political will on the part of the ZANUPF led government to implement crucial constitutional reforms that have a bearing on the configuration of IGR. The study also revealed that political party incongruence is a threat to intergovernmental coordination, integrated planning and collaborative development. Various recommendations were made from the study and these include that national government should expedite the implementation of the Constitution, codification of IGR by way of legislation and rationalization of fiscal transfers and intergovernmental fiscal equalization.
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  • Date Issued: 2017

Structural determinants of the HSP90-Fibronectin interaction and implications for fibrillogenesis

  • Authors: Chakraborty, Abir
  • Date: 2022-04-08
  • Subjects: To be added
  • Language: English
  • Type: thesis , text , Doctoral , PhD
  • Identifier: http://hdl.handle.net/10962/294572 , vital:57234
  • Description: Thesis embargoed. Release date April 2024. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2022
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  • Date Issued: 2022-04-08

HSP90 interacts with the fibronectin N-terminal domains and increases matrix formation:

  • Authors: Chakraborty, Abir , Boel, Natasha M-E , Edkins, Adrienne L
  • Date: 2020
  • Language: English
  • Type: text , article
  • Identifier: http://hdl.handle.net/10962/165407 , vital:41241 , https://doi.org/10.3390/cells9020272
  • Description: Heat shock protein 90 (HSP90) is an evolutionarily conserved chaperone protein that controls the function and stability of a wide range of cellular client proteins. Fibronectin (FN) is an extracellular client protein of HSP90, and exogenous HSP90 or inhibitors of HSP90 alter the morphology of the extracellular matrix. Here, we further characterized the HSP90 and FN interaction. FN bound to the M domain of HSP90 and interacted with both the open and closed HSP90 conformations; and the interaction was reduced in the presence of sodium molybdate. HSP90 interacted with the N-terminal regions of FN, which are known to be important for matrix assembly.
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  • Date Issued: 2020

The Role of HSP70/HSP90 Organizing Protein (Hop) in the Heat Shock Factor 1 (HSF1)-mediated Stress Response

  • Authors: Chakraborty, Abantika
  • Date: 2020
  • Subjects: Uncatalogued
  • Language: English
  • Type: Doctoral theses , text
  • Identifier: http://hdl.handle.net/10962/163204 , vital:41018 , doi:10.21504/10962/163204
  • Description: Molecular chaperones regulate cellular proteostasis. They control protein conformation and prevent misfolding and aggregation under both normal and stressful environments, ultimately resulting in cell survival. The project aimed to understand the role of the HSP70 – HSP90 Organizing Protein (Hop/STIP1) in the survival of stressed cells and the function of the stress-responsive transcription factor, Heat Shock Factor 1 (HSF1). HSF1 protein levels were significantly reduced in Hop-depleted HEK293T cells compared to controls by ELISA, western blot, and mass spectrometry. HSF1 transcriptional activity at the HSP70 promoter, and binding of a biotinylated HSE oligonucleotide under basal conditions were significantly reduced, consistent with the reduced levels of HSF1. In response to heat shock, HSF1 levels in Hop-depleted cells increased to that of controls, but there was still significantly lowerHSF1 transcriptional activity and HSE binding. Hop-depleted HEK293T cells were more sensitive than controls to the HSF1 inhibitor KRIBB11 and showed reduced short-term and long-term proliferation. Unlike the HSP90 inhibitor 17-DMAG, which had no effect, the HSP70 inhibitor JG98, further decreased the levels of HSF1 in Hop-depleted cells, suggesting a role for HSP70 in the Hop-mediated effects. There was punctate nuclear staining for HSF1 in Hop-depleted cells under both basal and heat shock conditions, as well as reduced nuclear localization and increased cytoplasmic accumulation of HSF1 in response to heat shock. Hop and HSF1 colocalized in cells, and HSF1 could be isolated in complex with Hop and HSP70. Loss of Hop reduced HSF1 in HSP70complexes but did not affect HSF1 abundance in HSP90 complexes. Hop-depleted cells showed reduced short-term and long-term survival compared to controls, an effect that was potentiated by the JG98 HSP70 inhibitor. Taken together, these data suggest that Hop regulation of HSF1activity is via a mechanism involving reductions in HSP70 interaction, as well as reduced nuclear localization, and DNA binding, and is consistent with reduced cellular fitness under basal and stress conditions. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2020
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  • Date Issued: 2020

Hop depletion reduces HSF1 levels and activity and coincides with reduced stress resilience:

  • Authors: Chakraborty, Abantika , Edkins, Adrienne L
  • Date: 2020
  • Language: English
  • Type: text , article
  • Identifier: http://hdl.handle.net/10962/165440 , vital:41244 , https://doi.org/10.1016/j.bbrc.2020.04.072
  • Description: Heat-shock factor 1 (HSF1) regulates the transcriptional response to stress and controls expression of molecular chaperones required for cell survival. Here we report that HSF1 is regulated by the abundance of the Hsp70-Hsp90 organizing protein (Hop/STIP1).
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  • Date Issued: 2020

Evaluation of the role of ethical leadership development on organisational performance: the case of Econet Wireless Zimbabwe

  • Authors: Chakonza, Hawkins
  • Date: 2020
  • Subjects: Econet Wireless Zimbabwe , Leadership , Leadership -- Case studies , Business ethics
  • Language: English
  • Type: Thesis , Masters , MBA
  • Identifier: http://hdl.handle.net/10962/146723 , vital:38551
  • Description: The study evaluated the role of ethical leadership development on the performance of the organisation. Econet Wireless Zimbabwe was employed as a case study. The objectives of the study were to: understand the role of ethical leadership development at Econet Wireless Zimbabwe on the day to day running of the business, understand the nature of the ethical leadership development at Econet Wireless, examine the factors influencing ethical leadership development at Econet Wireless, ascertain the role of ethical leadership development in enhancing organisational perfomance and efficiency and to investigate leaders’ perceptions on ethical leadership development on the organisation’s effectiveness. Social learning and social exchange theory informed the study. The research employed a qualitatve approache to improve the validity,reliability and credibility of the research findings. key informnat intervews and focus group of discussion and in-depth interviews were used as data collection instruments. The study found that ethical leadership development programmes improve the perfomance of orgnisations. That is, ethical leadeship development enbaled Econet Wireless Zimbabwe to achive disticinive competence, increase profit, have a large number of subscribers and also expanded among others. Furthermore, the study shows that an ethical leadership development approach brings significant value to the organisation and, suggests that ethical leaders are more influential in organizations than currently understood. The study found that leaders could influence ethics related outcomes in the organisation by engaging in behaviours that makes them perceived as credible, attractive and legitimate by their followers.
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  • Date Issued: 2020

Fast track land reform programme and women in Goromonzi district, Zimbabwe

  • Authors: Chakona, Loveness
  • Date: 2012
  • Subjects: Land reform -- Research -- Zimbabwe Land settlement -- Government policy -- Zimbabwe Land reform beneficiaries -- Zimbabwe Sex discrimination against women -- Zimbabwe Women's rights -- Zimbabwe
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:3317 , http://hdl.handle.net/10962/d1003105
  • Description: From the year 2000, land became the key signifier for tackling the unfinished business of the decolonisation process in Zimbabwe, notably by rectifying the racially-based land injustices of the past through land redistribution. This took the form of the Fast Track Land Reform Programme (FTLRP). However, the racialised character and focus of the FTLRP tended to mask or at least downplay important gender dimensions to land in Zimbabwe. Colonial and post-colonial Zimbabwe (up to 2000) had instigated, propagated and reproduced land ownership, control and access along a distinctively patriarchal basis which left women either totally excluded or incorporated in an oppressive manner. This patriarchal structuring of the land question was rooted in institutions, practices and discourses. Although a burgeoning number of studies have been undertaken on the FTLRP, few have had a distinctively gender focus in seeking to identify, examine and assess the effect of the programme on patriarchal relations and the socio-economic livelihoods of rural women. This thesis makes a contribution to filling this lacuna by offering an empirically-rich study of land redistribution in one particular district in Zimbabwe, namely, Goromonzi District. This entails a focus on women on A1 resettlement farms in the district (and specifically women who came from nearby customary areas) and on women who continue to live in customary areas in the district. My thesis concludes that the FTLRP is seriously flawed in terms of addressing and tackling the patriarchal structures that underpin the Zimbabwean land question.
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  • Date Issued: 2012

Effects of symmetry and the number of positive charges on the photocatalytic activity of indium phthalocyanines when embedded in electrospun fibers

  • Authors: Chakona, Gamuchirai , Nyokong, Tebello
  • Date: 2017
  • Subjects: To be catalogued
  • Language: English
  • Type: text , article
  • Identifier: http://hdl.handle.net/10962/188304 , vital:44743 , xlink:href="https://doi.org/10.1016/j.ica.2016.12.025"
  • Description: Photooxidations of bisphenol A and 4-chlorophenol were carried out in this study using two asymmetric indium(III) phthalocyanines photosensitizers: the first consisting of one quaternized 4-pyridyloxyl and no other substituents (complex 1) and the second containing three quaternized 4-pyridylsulfanyl units and one aminophenoxy (complex 3). The asymmetry of complex 3 is additionally caused by the presence of two linking atoms (O and S). The symmetrically substituted indium(III) phthalocyanine containing four quaternized 4-pyridyloxyl units (complex 2) was also studied. The complexes were embedded in electrospun polystyrene fiber for heterogeneous photocatalytic transformation of 4-chlorophenol and bisphenol A. When immobilized on electrospun fibers, complexes 1, 2 and 3 gave singlet oxygen values of 0.31, 0.39 and 0.50 in water, respectively. Complex 3 showed the best photocatalytic behavior with observed rate constants of 2.8 × 10−3 min−1 for bisphenol A and 3.8 × 10−3 min−1 for 4-chlorophenol.
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  • Date Issued: 2017

Minimum dietary diversity scores for women indicate micronutrient adequacy and food insecurity status in South African towns

  • Authors: Chakona, Gamuchirai , Shackleton, Charlie M
  • Date: 2017
  • Language: English
  • Type: article , text
  • Identifier: http://hdl.handle.net/10962/60930 , vital:27892 , http://dx.doi.org/10.3390/nu9080812
  • Description: The lack of dietary diversity is a severe problem experienced by most poor households globally. In particular; women of reproductive age (WRA) are at high risk of inadequate intake of micronutrients resulting from diets dominated by starchy staples. The present study considered the diets, dietary diversity, and food security of women aged 15-49 years along the rural-urban continuum in three South African towns situated along an agro-ecological gradient. A 48 h dietary recall was conducted across two seasons with 554 women from rural, peri-urban, and urban locations of Richards Bay, Dundee, and Harrismith. Minimum Dietary Diversity for WRA (MDD-W) were calculated and a dichotomous indicator based on a set of ten food groups was used to determine if women had consumed at least five food groups the previous 48 h to achieve minimum dietary intake for women. The mean (±sd) MDD-W for Richards Bay (3.78 ± 0.07) was significantly higher than at Dundee (3.21 ± 0.08) and Harrismith (3.36 ± 0.07). Food security and MDD-W were significantly higher in urban locations than in peri-urban or rural ones. There was lower dependence on food purchasing in Richards Bay compared to Dundee and Harrismith. The majority of women in Richards Bay practiced subsistence agriculture, produced a surplus for sale, and collected wild foods which improved dietary intake and food security. The peri-urban populations had limited dietary intake and were more food insecure because of high levels of poverty, unemployment, and lack of land. Peri-urban dwellers are therefore more sensitive to changes in incomes and food prices because they lack safety nets to absorb income or price shocks as they purchase more, rather than growing their own food. This compromises dietary diversity as they have limited access to diverse foods.
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  • Date Issued: 2017
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