Leisure time and holiday aspirations of black upper middle class in East London
- Authors: Ngozwana, Baselwa
- Date: 2016
- Subjects: Social classes -- South Africa -- Eastern Cape Lifestyles -- South Africa
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6380 , vital:29600
- Description: This study examined the holiday and leisure aspirations of the new upper black middle class in East London using qualitative approach. The study investigated aspirations of the black middle class on international travels through the lens of Veblen’s (2002) conspicuous consumption theory which entails that black middle class citizens spend in order to assert their status and belongingness to the middle class. The aim of the study was to understand their holiday and leisure aspirations, what the idea is behind or what motivates their international travels, their travel experiences and class affirmations. This exploration included the challenges and frustrations such as Ebola scare, racism, issues with foreign exchange and variations from these international visits. Data was collected through interviews with upper black middle class who are based in the Buffalo City Metro Municipality in East London, Eastern Cape. Findings from this study revealed that consumption patterns of the emerging black middle class to diverge substantially from the other groups, in terms of greater signalling of social status via visible consumption and preoccupation with reducing an historical asset deficit. Various themes emerged as meanings attached to international travelling and leisure by those black middle class international travellers. These are sense of wealth, status, religion, self-esteem, sense of reward, bonding with friends and conspicuous/visible consumption.
- Full Text:
- Date Issued: 2016
A study of lanthanide complexes with di-2-pyridyl ligands
- Authors: Coetzee, Louis-Charl Cloete
- Date: 2016
- Subjects: Rare earth metals
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/4731 , vital:20659
- Description: The ligands di(2-pyridyl) ketone (DPK) and cis-1,2 di(2-pyridyl) ethylene (DPE) are N,N,Odonor ligands that can undergo nucleophilic addition and become more flexible for coordination. The reaction between the lanthanide thiocyanate salt and DPK gave rise to seven novel complexes of the general formula [Ln(NCS)3(DPKOH)3], where Ln = La, Ce, Nd, Eu, Tb, Dy and Ho. 1H NMR spectroscopy verified that the ligand underwent nucleophilic addition upon coordination. This was further confirmed using UV-Vis spectroscopy which showed a shift in the absorption bands due to conjugation of electrons within the pyridyl ring upon coordination. UV-Vis spectroscopy was also utilised to test the covalent character of the neodymium and holmium complexes. X-ray diffraction and IR spectroscopy showed that three DPK ligands coordinated bidentately through a pyridinic nitrogen and a hydroxyl oxygen, while three isothiocyanato molecules completed the coordination environment around each metal. Furthermore, X-ray diffraction also revealed that these complexes are isostructural, ninecoordinate and the polyhedron which encloses each ion is of trigonal tricapped prismatic shape with D3h symmetry. Micro-analysis on all the complexes, except lanthanum and holmium confirmed the molecular formulae produced from the crystallographic data of each complex. The reaction between the lanthanide thiocyanate salt and DPE produced poor quality crystals which could not be detected by X-ray diffraction. The lanthanide salts used for this reaction were lanthanum, neodymium, europium, dysprosium and holmium. Upon coordination, conductivity measurements detected the presence of lanthanide ions in each solution. 1H NMR and IR spectroscopic studies showed that the ethylenic moiety of DPE underwent nucleophilic addition upon coordination. UV-Vis spectroscopy further confirmed nucleophilic addition upon coordination due to shifts in absorption bands. IR spectroscopy verified the possibility of a bidentate coordination to each metal through a pyridinic nitrogen and a hydroxyl oxygen as well as a monodentate coordination through isothiocyanato ligands. A micro-analysis on all the complexes provided the molecular formulae that can best fit each complex. The effect of the solvent molecules on the bonding parameters of the lanthanum complex was investigated. An analysis of the results produced from crystallographic data revealed the presence of intermolecular forces which interacted and stabilised the complex.
- Full Text:
- Date Issued: 2016
Business ethics in Ugandan small and medium-sized enterprises
- Authors: Mayanja, Jamiah
- Date: 2016
- Subjects: Business ethics -- Uganda , Small business -- Uganda
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/8521 , vital:26400
- Description: SMEs have been acknowledged by governments globally as a prime source of economic growth and development. In Africa there has been a noticeable increase in the number of SME establishments. In Uganda, SMEs are the most popular business choice and play a major role in the national economy. Although SMEs significant economic contributions are generally acknowledged, being ethical and successful has become a challenge, as many SMEs in Uganda have not fully adopted and integrated ethics into their business strategies. Understanding the reasons for the increased unethical behaviour in SMEs is central to their continued business success. The primary objective of the study was to investigate the factors that influence ethical business conduct in Ugandan SMEs. From a comprehensive literature review, three main independent variables (staff-, business- and external environment factors) were identified as variables influencing ethical business conduct (dependent variable) of SMEs. A hypothetical model was developed to determine whether the independent variables have an influence on the dependent variable. Twelve hypotheses were formulated to test the relationships between three staff factors, five business factors, four external environment factors and ethical business conduct. The study sought the perceptions of SME owners or managers in the Kampala District and utilised the quantitative research paradigm. A survey was conducted with the aid of a structured self-administered questionnaire distributed by three fieldworkers. A combination of convenience and snowball sampling was utilised. The final sample comprised 384 respondents. The validity of the measuring instrument was ascertained by using exploratory factor analysis. The Cronbach‟s alpha values for reliability were calculated for each of the factors identified during the exploratory factor analysis. A total of ten valid and reliable factors were retained. Pearson product-moment correlation and multiple regression analysis were used to test the correlation and statistical significance of the relationships hypothesised between the various independent and dependent variables. One statistically significant relationship was found between the staff factors (employee attitude) and ethical business conduct. Two statistically significant relationships were found between the business factors (knowledge acquisition and management practices) and ethical business conduct. Three statistically significant relationships were found between the external environment factors (legal requirements, industry norm and media power) and ethical business conduct. External environmental factors seem to have a greater influence on SME ethical business conduct in Uganda. Multivariate Analysis of Variance (MANOVA) was used to identify if significant relationships exist between the eight demographic variables and seven reliable and valid independent variables. Furthermore, post-hoc Scheffé tests identified where the significant differences occurred between the different categories. Cohen‟s d-values were calculated in order to assess the practical significance of the mean scores. A total of twelve practical significant relationships were identified. SME owners or managers should consider employing staff with the right attitude to uphold sound ethical business values. They should implement ethical management practices to promote ethical business conduct amongst employees and ensure that employees are made aware of what is regarded as acceptable ethical business behaviour. SME owners or managers should adhere to legal requirements and industry norms to be known as businesses exhibiting ethical behaviour and utilise media to instil and guide ethical values in employees. Lastly, they must pay attention to the role that demographical variables such as: gender, level of education, current employment status, number of years in business and number of employees, play in behaving ethically in business.
- Full Text:
- Date Issued: 2016
Influence of argon-implantation on conventional and phototransferred thermoluminescence of synthetic quartz
- Authors: Nsengiyumva, S , Chithambo, Makaiko L , Pichon, L
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124732 , vital:35656 , DOI: 10.1080/10420150.2016.1194412
- Description: Conventional and phototransferred thermoluminescence of crystalline synthetic quartz implanted with 70 keV Ar ions at fluences in the range 1 × 1014–5 × 1015 ions/cm2 is reported. The glow curves, recorded at 5°C/s from beta-irradiated samples of similar mass, show a prominent peak between 100°C and 120°C. The thermoluminescence intensity of all implanted samples was greater than that of the unimplanted one. The increase in sensitivity is attributed to a corresponding increase in the concentration of point defects, as a result of the implantation, which act as electron traps or recombination centres. Kinetic analysis carried out using the peak shape, whole glow-peak and curve-fitting methods produced values of the activation energy, frequency factor and order of kinetics that are generally independent of implantation fluence. This result suggests that implantation did not necessarily affect the nature of the electron traps. With respect to phototransferred thermoluminescence, it was observed that it only appeared in the sample implanted at the highest fluence of 5 × 1015 ions/cm2. This may be so because the concentration of deep traps produced as a result of implantation at low fluence is too low to give rise to phototransferred thermoluminescence. The intensity of the phototransferred thermoluminescence goes through a peak with illumination time. We attribute this behaviour to the relative concentration of holes at recombination centres and phototransferred electrons at shallow traps.
- Full Text: false
- Date Issued: 2016
The taxability of rewards from suppliers
- Authors: Akoonjie, Ahmed Hassen
- Date: 2016
- Subjects: Taxation -- South Africa Industrial procurement -- South Africa
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10948/11253 , vital:26902
- Description: Successful business in today’s competitive climate thrives on building relationships with one’s suppliers, clientele and employees. Businesses, as a form of strategic engagement, extend their appreciation and recognition to these key role players with rewards (or incentives) such as prizes, holidays and free goods and/or services. This treatise examines the taxation implications of such rewards and determines that the market value thereof should be taxed in the hands of their recipients. This study also investigates possible indicators (or points of reference) to establish such market value and suggests alternate values that may be ascribed thereto for inclusion in the recipient’s gross income. Where the rewards are passed onwards by the recipient to its employees, fringe benefit consequences are occasioned and this study observes that the market value thereof should be taxed in the employees’ hands. The study goes on further to consider the position of the supplier (or provider) of the rewards and finds that the supplier is entitled to a deduction of the cost of the rewards it has provided to its clients or customers. This study also reflects upon the penal implications of not including the market value of rewards in gross income and notes that such non-inclusion constitutes a fiscal loss and is subject to an understatement penalty. Key recommendations on how such omission may be addressed by the tax authorities are also presented.
- Full Text:
- Date Issued: 2016
Marie Curie : a psychobiography
- Authors: Roets, Elmeret
- Date: 2016
- Subjects: Curie, Marie -- 1867-1934 -- Psychology , Women chemists -- Poland -- Biography , Scientists -- Biography
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3269 , http://hdl.handle.net/10962/d1020326
- Description: While researchers debate the value of psychobiographical research, interest in this area is growing on a national and international basis. Every year, the number of psychobiographical studies at universities in South Africa is growing. Psychobiographical research is qualitative research that utilises psychological theory to explore and describe the lives of extraordinary individuals. The primary aim of this psychobiography was to examine the life of Marie Curie (1867–1934) by employing developmental psychologist and psychoanalyst Erik Erikson’s (1959) theory of psychosocial personality development. Marie Curie was chosen as the research subject because of the researcher’s personal interest and the subject’s prominence as a female scientist. She was a Polish-born and naturalised French scientist who conducted pioneering research on radioactivity. Marie Curie’s ground-breaking discoveries changed the way scientists think about matter and energy and introduced a new era in medical knowledge and the treatment of disease. Her life exemplifies a love of science, commitment, and perseverance. Data were collected from several primary and secondary sources on Marie Curie’s life. The researcher developed a data-collection and analysis matrix to facilitate the systematic collection of data and analysis according to Erikson’s stage theory of psychosocial personality development. This psychobiography suggests that unresolved infantile and early childhood crises gave rise to personality traits that eventually contributed to Curie’s extraordinariness. In the case of Curie, personality traits that are often regarded as atypical or malignant, ironically encouraged perseverance, creativity, and productivity. This study complements the psychobiographical studies done in South Africa on extraordinary individuals. It demonstrated the value of psychobiographical research as a teaching instrument, revealed the usefulness of Erikson’s theory, and illustrated the uniqueness of individuals.
- Full Text:
- Date Issued: 2016
Risk analysis and management systems in South African construction project management practices
- Authors: Cook, Iain Murray
- Date: 2016
- Subjects: Construction projects -- Management , Construction industry -- Risk management , Project management -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/3469 , vital:20433
- Description: Risk management (RM) should be seen as one of the most important functions in the South African built environment. Without the effective management of the risks associated with the industry, the noble vision of a sector that is efficient, profitable, and sustainable cannot be achieved. By embracing tried and tested policies that successfully mitigate risk, industry stakeholders will achieve many project successes, and will outlast any competitors that choose to ignore, or are ignorant of the fact, that the negative impact risk has on projects is inversely proportional to the level of RM employed. Construction Project Management (CPM) practices, realising that there are excellent business opportunities across South Africa’s borders, and faced with a competitive South African market, are engaging with developers and government entities involved in cross border projects in the hope of securing these potentially lucrative African projects. With this move into Africa comes increased uncertainty and risk for these CPM practices, and other project stakeholders. Similarly, CPM practices that have made the strategic decision to remain operational only within South Africa’s borders, are faced with a competitive and complex built environment and industry, made increasingly challenging by a weakening economy, exacerbated by industrial strikes, infrastructure deficiencies and a decrease in industry skill levels. This study reports on Project Managers’ (PMs’) perceptions of project failures and inefficiencies resulting from inadequate RM on projects, including the RM methodologies currently being employed. The study focused on perceptions of PMs who operate within South Africa’s borders, PMs that operate across border into other African countries, as well as PMs who operate exclusively within South Africa’s built environment framework. A study was undertaken incorporating qualitative methodologies via a normative survey. The survey was split into three main phases. Phase one employed the use of a pilot survey executed with the objective of further investigating the main sub-problems to gain more insight into the related issues and challenges. For the pilot survey, PMs were selected based on their engagement in CPM activities within South Africa as well as across South Africa’s borders into other African countries. Phase two of the main survey, with the sample stratum being the Association of Construction Project Managers (ACPM), was aimed at PMs within the ACPM who have engaged, or are engaging, in CPM activities both within South Africa’s borders as well as across South Africa’s borders into other African countries. Phase 3 of the main survey, with the sample stratum being the ACPM, was aimed at PMs within the ACPM who have engaged, or are engaging, in CPM activities within South Africa’s borders only and have not engaged in cross border activities. Survey findings identified the commercial sector and value of the projects undertaken by the practices, the level of risk associated with different client typologies, the link between inadequate RM and project inefficiency and failure, and the importance of RM on projects. Findings also identified that RM methodologies are employed by CPM practices, and that CPM practices generally endeavour to create a culture of risk awareness amongst employees. Further findings indicated that CPM practices may not always understand the risks associated with new industry sectors, regions or countries that they are considering operating within, and that that there is room for improvement regarding the effectiveness of current RM systems. Survey findings also indicated that risk is not always transferred to the correct project stakeholder most suited to managing the risk, and CPM practices are not always able to accurately quantify the costs associated with project risk. Furthermore, it was identified that CPM practices do not always undertaken risk assessments (RAs) at the correct project stage resulting in inadequate risk contingencies allowances, regular risk reviews are not always undertaken for projects, project pre-mortems are seen as valuable tools by CPM practices as a method to reduce future risk, and project post-mortems relative to ‘lessons learnt’ are not always undertaken. Conclusions outline the link between effective RM, project inefficiencies and project failure, as well as the increase or decrease in risk relative to ineffective or effective use of risk identification and management methodologies for time, cost, and quality factors respectively. Conclusions also outline the fact that although CPM practices generally understand the link between RM and project success, they are not always able to fully comprehend the risks associated with new industry sectors, regions or cross border countries. This indicates that without the adequate identification of risk, the RM process or steps that follow the qualitative risk identification process will have little or no value. This is indicative of the requirement for professional associations to consolidate risk data for industry activities with the aim of improving the level of RM industry wide. Recommendations highlight the importance of the compiling of sector specific risk registers, compiled by the South African Council for the Project and Construction Management Professions (SACPCMP) with registered member input, made available to all PMs via the SACPCMPs online database. Further recommendations include: the engendering, by senior management of CPM practices; a healthy ‘risk aware’ culture, by promoting RM practices aligned with best practice methodologies; the implementation of well balanced and formal RM systems throughout the CPM practice, with the aim of achieving effective RM without overburdening PMs with unnecessary documentation or ‘paperwork’; the attendance of risk conferences and workshops by all CPM practices, aimed at specifically identifying challenges that exist with RM and methods that can be employed to improve the status quo; the attendance of formal risk training courses, by all CPM practices, aimed at improving the knowledge base of PMs relative to effective RM, and the appointment of risk professionals, driven by the monetary value and risk levels of the project, to undertake the RM process and unburden PMs from the task, allowing PMs to concentrate on the other project knowledge areas.
- Full Text:
- Date Issued: 2016
Colonised minds?: post-development theory and the desirability of development in Africa
- Authors: Matthews, Sally
- Date: 2017
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142213 , vital:38059 , DOI: 10.1080/01436597.2017.1279540
- Description: While post-development theory is very concerned with the ways in which development has impacted upon the countries of the Global South, there has been relatively little written on post-development theory from an African perspective. This paper identifies some of the ways in which post-development theory fails to adequately understand the African experience of development. In particular, I explore the difficulty that post-development theory confronts when faced with the continued desire on the part of many people in Africa for development.
- Full Text:
- Date Issued: 2017
A situational survey of Siyazondla homestead food production proogramme and food security, poverty alleviation in selected communities of Nkonkobe Local Municipality of the Eastern Cape
- Authors: Phezisa, Bulelani
- Date: 2016
- Subjects: Food security -- South Africa -- Eastern Cape Food supply -- South Africa -- Eastern Cape Poverty -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2392 , vital:27795
- Description: People living in rural areas are continually losing their value, dignity, ubuntu and rural nature of livelihood. Agriculture in these areas characterises the nature of rural livelihood. Traditionally, people living in rural areas were highly dependent on their production from gardens for food, income (barter exchange), and other social activities. Nowadays, that motive and interest is no longer operating at its full potential, so to revive that history and nature, household (homestead) production, certain programmes were introduced to greatly improved the standard of living of the poor, and it has proved to have an impact as it has generated income, and created food stability and employment through the project. Home gardens are there and can be used as the method and the strategy toward improvement and development of the people around the world, more especial to developing countries. Certain programmes that are initiated for acting against the challenges faced by rural people have failed to materialise and operate on their fully potential. The Siyazondla homestead food production programme is the one of the programmes practiced by the government of South Africa to act against such challenges, affecting especially poor people. This study was aimed at assessing the role of Siyazondla homestead food production programme in food security and poverty alleviation in selected communities of Nkonkobe local municipality of the Eastern Cape. It took the form of a case study in Msobomvu, Ngcothoyi and Binfield locations. The Nkonkobe local municipality is demarcated into five major areas: Middledrift, Alice, Seymour, Fort Beaufort and Balfour. The study sample was collected from beneficiaries and non-beneficiaries of the Siyazondla programme. The sample size was 90 respondents, from which in one area, a 15 beneficiaries and anticipated non- beneficiaries were targeted. The target sample from each village was collected from 15 beneficiaries and non-beneficiaries. Many farmers from the study area benefited from the programme, though a limited number was targeted. Therefore, a probability sampling was applied and employed, where there were more than required number of beneficiaries of the programme and anticipated beneficiaries of the programme. The study was effectively and efficiently carried out and achieved with the use of personal interviewing of the recipients by use of questionnaires. The study was analysed by a computerised programme of analyses called SPSS v.2.1 and excel. The objectives of the study based on food security, poverty alleviation and reduction, and also improving nutritious status. The finding of the research clearly indicates the outcome of the programme on livelihood basis of the beneficiaries. Some of the objectives of the programme were achieved, such as food security and nutritious levels, whilst other objectives had shortcomings. The finding of the study also shows that programme, though had original benefits but also there are the anticipated and probable benefits of the programme Siyazondla, such as income, skill of farming, improving social status, and there are some possibilities when the programme is properly implemented. Though the majority of people are concentrated in rural areas, the programme on its own had shortcoming to meet needs of the whole population, as food insecurity, unemployment and poverty are taking its course. The programme on its own had shortcoming such as lack of adequate resource and as results, very few people benefited from the programme. Another shortcoming of the programme is the effectiveness of agricultural extension personnel to certain aspect such as marketing of farmers produce and proper monitoring and evaluation of the programme. Therefore, in the long run programmes of this nature are promising, there will be some development and improvement toward growth and better standards of livelihood. It is through this study that had to assess the structure, procedure, strategies implemented of the programme and impact of the programme to both beneficiaries and anticipated beneficiaries of the programme.
- Full Text:
- Date Issued: 2016
Evaluating farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients in Sheshegu communal rangelands of the Eastern Cape, South Africa
- Authors: Tokozwayo, Sive
- Date: 2016
- Subjects: Range management -- South Africa -- Eastern Cape Brush -- Control -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2733 , vital:28066
- Description: Communal rangelands occupy 13 percent of the agricultural land in South Africa, and these rangelands serve as a source of feed to livestock. These areas are threatened by bush encroachment due to poor rangeland management. This study was conducted in Alice (Sheshegu communal area) and the objective was to assess farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients. Structured questionnaires were used to assess indigenous knowledge of communal farmers on the impact of bush encroachment on rangelands. Fourty (40) respondents from households who owned livestock were randomly selected and interviewed at Sheshegu village. About 89 percent of communal farmers perceived that change of their grasslands to encroached savannas was caused by unreliable rainfall, prolonged drought, and poor rangeland management. Rangeland assessment was performed at four sites (Scattered, Moderate, Mixed and Dense bushland. 100mx50m were demarcated per site, and four 100m transects were laid parallel to each other, 30m apart. The step point method was used to determine both species composition and basal cover. Biomass production was determined by harvesting forage within randomly-paced a 0.25m2 quadrats. Aristada congesta and Eragrostis obtuse were the most dominating grass species in dense, mixed and moderate bushland. Scattered bushland was dominated by Themeda triandra. Biomass production increases with increase in bush density and basal cover improved from winter to summer season. Increaser grass species increase with the increase in bush density, this indicated that the rangeland was poorly managed and palatable species were being replaced by less palatable ones. Species composition of woody plants was determined within a 200m2 belt transect in all sites. Maytenus polycantha, Aloe ferox, Erehia rigida and lucas capensis were the most dominant woody species in Mixed bushland while Acacia karroo was most dominated in Scattered, Moderate and Dense bushland. Woody density showed no significant differences (P> 0.05) between Dense, Mixed and Moderate bushland. Woody plant density in all these sites exceed 2500 plants/ha, which showed that the rangeland was encroached with woody plants. Soil nutrient content was determined for each site. Five samples of soil were collected per site to test the effect of bush encroachment on soil nutrient composition. The concentration of nitrogen and soil pH increased with increase in bush density, while that of potassium, phosphorous, magnesium, sodium and sulphur showed no significant differences (P>0.05) across all homogenous vegetation units. The study revealed that Sheshegu communal farmers are aware of both negative and positive effects of encroachment on their community, and also argue that the government is not willing to support communal farmers in the process of reducing the spread of bush encroachment. It was concluded that the concentration of nitrogen and soil pH increased with increasing bush density and that bush encroachment had negative effects on herbaceous species. It was recommended that communal farmers should be formulating rangeland rules and regulations to manage rangeland resources. Rangeland rules and regulations should be based on the management of livestock management during grazing and harvesting of rangeland resources. The harvesting of woody and good timing of application of fire can also reduce the spread of bush encroachment. Veld resting and rotational grazing can also improve fuel load which is required for burning of woody plants. Furthermore, Department of agriculture should also provide workshops or training to communal farmers use different approaches or cheap ways of reducing bush encroachment and conserving rangeland resources.
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- Date Issued: 2016
The development of rhenium nanoradiopharmaceuticals
- Authors: Ntsimango, Songeziwe
- Date: 2016
- Subjects: Rhenium -- South Africa Radiopharmaceuticals -- South Africa , Nanoparticles -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/13017 , vital:27144
- Description: The dissertation details the experimental work on the attempt to develop rhenium(V)phthalocyanine complexes directly from its +7 oxidation state (perrhenate). Different reducing agents (PPh3, Na2S2O5 and NaBH4) were employed and consequently, different results were acquired, such as rhenium(V)-mediated oxidative hydrolysis of the phthalocyanines (Pcs), the formation of a rhenium-phthalocyanine complex and phthalocyanine-capped nanoparticles. The rhenium nanoparticles that were formed were optimized from a synthesis point of view and, cancer localizing ability of the rhenium nanoparticles was investigated. The complexes were synthesized through direct metalation of pre-formed metal-free phthalocyanines using the “cold isotopes” of the rhenium metal. Rhenium nanoparticles (Re NPs) were synthesized in aqueous saline medium so as to imitate the environment on which Re is produced from its reactor. The nanoparticles (NPs) were capped with phthalocyanines which were covalently biofunctionalized with a folic acid moiety to enhance the targeting ability of the Re NPs. These NP systems were characterised with techniques such as ultraviolet-visible UV-Vis spectroscopy and transmission electron microscopy TEM. Cytotoxicity of the NPs was tested against four different cell lines and subsequently their cytotoxicity profiles were elucidated, and the profiles shown a dose-dependent responsealthough the results in some cell lines were unclear. Their fluorescence properties were also studied to provide photophysical information for investigation of their tumor localization using human cancer cells lines via confocal fluorescence microscopy studies. Particle size effect on localization of NPs was also investigated using confocal fluorescence and TEM. Two sizes were chosen (10 and 50 nm), and the smaller NPs (10 nm) were found to exhibit stronger fluorescence properties than the 50 nm NPs, and they were also found to have a better localization ability than the 50 nm NPs. Finally, their tumor and organ biodistribution studies will be carried out using micro-SPECT kits and model mice (using the “hot” isotopes in a radiopharmacy laboratory).
- Full Text:
- Date Issued: 2016
Localizing selected endocytosis protein candidates in Plasmodium falciparum using GFP-tagged fusion constructs
- Authors: Basson, Travis
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2680 , vital:20316
- Description: Malaria is a mosquito-borne infectious disease caused by several obligate intracellular protozoan parasites in the Plasmodium genus, with Plasmodium falciparum causing the most widespread cases and malaria deaths. In 2013 there were approximately 190 million cases of the disease and between 584,000 and 855,000 deaths. It is essential to identify novel drug targets and develop novel drug candidates due to the increase in resistance of P. falciparum parasites to the current arsenal of antimalarial drugs. Endocytosis is an essential process in eukaryotic cells in which the external environment is internalized by the cell in order to obtain various particles from the extracellular space. This extracellular cytoplasm is internalized in membrane-bound invaginations at the plasma membrane. During the blood stage of malaria infection, the parasite requires nutrients from the host red blood cell. To obtain these nutrients, the parasite internalizes haemoglobin in large amounts and degrades it in an acidic, lysosome-like organelle, known as the digestive vacuole. Whilst the exact molecular mechanism of malaria parasite endocytosis is not yet fully understood, a number of proteins have been suggested to be involved. The most expedient approach in identifying candidate endocytosis proteins is to investigate parasite homologues of proteins known to be involved in endocytosis in mammalian cells. The three proteins selected for investigation in this study were the P. falciparum homologues of coronin, dynamin 2, and μ4. The coding sequences for the candidate endocytosis proteins were amplified by PCR and cloned into the pARL2-GFP expression vector. P. falciparum 3D7 parasites were transfected with these vectors and the episomal expression of full-length GFP-tagged fusion protein was confirmed by Western blot analysis using commercially available anti-GFP antibodies. Microscopic analysis of live parasites using fluorescence and confocal microscopy was used to determine the localization of the candidate endocytosis proteins. Coronin appeared to display diffuse cytoplasmic GFP localization during the trophozoite stage, arguing against a role in endocytosis. However, distinct localization during the schizont stage at what appears to be the inner membrane complex was observed. Coronin is thus likely required to coordinate the formation of the actin network between the merozoite IMC and the plasma membrane on which the glideosome is dependant for generating the motile forces required for the merozoite motility and invasion of RBCs. Dynamin 2 displayed localization at three potential locii: the parasite periphery (plasma membrane), punctuate regions within the cytoplasm (potentially at membrane bound organelles) and at the parasite food vacuole. The data suggested that dynamin 2 is involved in endocytosis and membrane trafficking in a similar manner to classical dynamins, potentially as a vesicle scission molecule at the plasma membrane, mediating vesicle formation at the food vacuole to recycle membrane to the plasma membrane, and possibly mitochondria organelle division. μ4 displayed transient localization, cycling between cytosolic localization, and localization to distinct regions at the plasma membrane and the food vacuole. Localization of Pfμ4 to the plasma membrane is indicative of a role for μ4 as a part of an adaptor protein (AP) complex which may be responsible for recruitment of clathrin to initiate endocytosis in a manner similar to mammalian AP-2. As was observed with PfDYN2, Pfμ4 localizes to the FV, which suggests that Pfμ4 forms part of a coat complex that mediates the formation of vesicles that recycle membrane from the FV to the parasite plasma membrane. This study showed that expressing proteins as full-length GFP-tagged fusion constructs is an effective approach in the early stages of determining the localization and function of P. falciparum proteins in vitro, and distinguishing between candidates that have a potential role in endocytosis and those that are unlikely to do so.
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- Date Issued: 2016
Aggregation control of robust water-soluble zinc (II) phthalocyanine-based photosensitizers
- Authors: Ikeuchi, Takuro , Mack, John , Nyokong, Tebello , Kobayashi, Nagao , Kimura, Mutsumi
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239709 , vital:50757 , xlink:href="https://doi.org/10.1021/acs.langmuir.6b03552"
- Description: A water-soluble zinc phthalocyanine (ZnPc) complex with four negatively charged electron-withdrawing sulfonic acid substituents at the nonperipheral positions (α-ZnTSPc) is found to have a high singlet oxygen (1O2) quantum yield and exhibits high photostability. The formation of aggregates is hindered and the highest occupied molecular orbital is significantly stabilized, making α-ZnTSPc potentially suitable for its use as a photosensitizer for photodynamic therapy and photoimmunotherapy. Atomic force microscopy (AFM) reveals that mixtures of the negatively charged α-ZnTSPc complex with a similar positively charged ZnPc were found to result in the self-assembly of one-dimensional accordion-like fibers. Supramolecular fibers can be formed in aqueous solutions through intermolecular electrostatic and donor–acceptor interactions between the two water-soluble ZnPcs.
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- Date Issued: 2016
Detecting derivative malware samples using deobfuscation-assisted similarity analysis
- Authors: Wrench, Peter Mark
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/383 , vital:19954
- Description: The overwhelming popularity of PHP as a hosting platform has made it the language of choice for developers of Remote Access Trojans (RATs or web shells) and other malicious software. These shells are typically used to compromise and monetise web platforms by providing the attacker with basic remote access to the system, including _le transfer, command execution, network reconnaissance, and database connectivity. Once infected, compromised systems can be used to defraud users by hosting phishing sites, performing Distributed Denial of Service attacks, or serving as anonymous platforms for sending spam or other malfeasance. The vast majority of these threats are largely derivative, incorporating core capabilities found in more established RATs such as c99 and r57. Authors of malicious software routinely produce new shell variants by modifying the behaviours of these ubiquitous RATs, either to add desired functionality or to avoid detection by signature-based detection systems. Once these modified shells are eventually identified (or additional functionality is required), the process of shell adaptation begins again. The end result of this iterative process is a web of separate but related shell variants, many of which are at least partially derived from one of the more popular and influential RATs. In response to the problem outlined above, the author set out to design and implement a system capable of circumventing common obfuscation techniques and identifying derivative malware samples in a given collection. To begin with, a decoder component was developed to syntactically deobfuscate and normalise PHP code by detecting and reversing idiomatic obfuscation constructs, and to apply uniform formatting conventions to all system inputs. A unified malware analysis framework, called Viper, was then extended to create a modular similarity analysis system comprised of individual feature extraction modules, modules responsible for batch processing, a matrix module for comparing sample features, and two visualisation modules capable of generating visual representations of shell similarity. The principal conclusion of the research was that the deobfuscation performed by the decoder component prior to analysis dramatically improved the observed levels of similarity between test samples. This in turn allowed the modular similarity analysis system to identify derivative clusters (or families) within a large collection of shells more accurately. Techniques for isolating and re-rendering these clusters were also developed and demonstrated to be effective at increasing the amount of detail available for evaluating the relative magnitudes of the relationships within each cluster.
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- Date Issued: 2016
Preference for C4 shade grasses increases hatchling performance in the butterfly, Bicyclus safitza
- Authors: Nokelainen, Ossi , Ripley, Bradford Sherman , Van Bergen, Erik , Osborne, Colin P , Brakefield, Paul M
- Date: 2016
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61424 , vital:28025 , http://onlinelibrary.wiley.com/doi/10.1002/ece3.2235/full
- Description: The Miocene radiation of C4 grasses under high-temperature and low ambient CO2 levels occurred alongside the transformation of a largely forested landscape into savanna. This inevitably changed the host plant regime of herbivores, and the simultaneous diversification of many consumer lineages, including Bicyclus butterflies in Africa, suggests that the radiations of grasses and grazers may be evolutionary linked. We examined mechanisms for this plant–herbivore interaction with the grass-feeding Bicyclus safitza in South Africa. In a controlled environment, we tested oviposition preference and hatchling performance on local grasses with C3 or C4 photosynthetic pathways that grow either in open or shaded habitats. We predicted preference for C3 plants due to a hypothesized lower processing cost and higher palatability to herbivores. In contrast, we found that females preferred C4 shade grasses rather than either C4 grasses from open habitats or C3 grasses. The oviposition preference broadly followed hatchling performance, although hatchling survival was equally good on C4 or C3 shade grasses. This finding was explained by leaf toughness; shade grasses were softer than grasses from open habitats. Field monitoring revealed a preference of adults for shaded habitats, and stable isotope analysis of field-sampled individuals confirmed their preference for C4 grasses as host plants. Our findings suggest that plant–herbivore interactions can influence the direction of selection in a grass-feeding butterfly. Based on this work, we postulate future research to test whether these interactions more generally contribute to radiations in herbivorous insects via expansions into new, unexploited ecological niches.
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- Date Issued: 2016
Exploring the influence of learners’ participation in an after-school science enrichment programme on their disposition towards science: a case study of Khanya Maths and Science Club
- Authors: Agunbiade, Esther Arinola
- Date: 2016
- Subjects: Mathematics -- Study and teaching Science -- Study and teaching After-school programs Academic achievement
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/334 , vital:19949
- Description: The ongoing advancement of science and technology is creating an increasing need for more entrants into science oriented careers. However, numerous studies have fueled growing concerns regarding the poor achievement of learners in science. Over the years, science education researchers have emphasized the importance of the affective domain of learning as a central component of strategies used to address learners’ lack of interest and poor achievement in science. In the literature, the affective domain is characterized by constructs such as disposition, attitude, interest, and motivation. Studies showing a correlation between the affective domain and academic achievement suggest that nurturing a positive disposition towards science is an antecedent to learners’ improved science achievement and entering science fields. This study focuses on the ‘disposition’ aspect of the affective domain, and follows in the path of earlier studies which use the term interchangeably with ‘attitude’. Learners’ experiences in a particular science education environment influence the development of a positive or negative disposition towards science. However, there is a need to explore the factors in the learning environments which influence learners’ disposition towards science. Previous studies have shown that the informal science environment may influence learners’ disposition towards science. One example of an informal science environment is the Khanya Maths and Science Club, which is an after-school science and mathematics enrichment programme in Grahamstown, South Africa. This study explores the influence of learners’ participation in an informal science education environment on their dispositions towards science, using the case of the Khanya Maths and Science Club. This study views disposition through the constructivist-developmental lens. The community of practice elements from situated learning theory is drawn on to explore how learners’ disposition can be influenced by their interactions in the context of the Khanya Maths and Science Club. The pragmatic paradigm is adopted, which considers how well the research tools work to provide answers to the research questions. This thus, provides an avenue for exploring how learners’ disposition towards science is influenced and what factors influenced their shift in disposition through their participation in the club. A mixed-methods approach is employed when focusing on the affective domain sub-constructs of: enjoyment of science, interest in science and perception of science. These are sub-scales in the test of science related attitude (TOSRA) questionnaire which was adapted for use in measuring learners’ attitude before and after 16 weeks of participating in the science club. The particular mixed-methods approach selected can be summarized as quan QUAL since the method is primarily qualitative, but sequential with the quantitative phase preceding the qualitative phase. The TOSRA questionnaire was used as the quantitative data collection instrument while semi-structured interviews and learners’ journal entries were the qualitative data collection instruments. The results revealed significant shifts in learners’ perception of, interest in science and enjoyment of science though interest in science and enjoyment of science shifted appreciably in a positive direction more than the perception of science. It was also found that learners’ attitude towards science was influenced by; instructional characteristics, facilitators/environmental characteristics, learners making connection between science and everyday life and learners’ perceived difficulty of science. These factors variably influenced their attitude towards science in the club, corroborating what had been found in similar studies. This study corroborates what the literature offers for achieving effective outcomes in Afterschool science enrichment programmes. It contributes to the growing body of literature on features for quality outcomes in Afterschool science enrichment programmes. This study also makes a theoretical contribution to science education research particularly with regard to how the emergence of a community of practice framework in the club activities provide useful information for planning club activities and the analysis of learners’ evolving disposition towards science. Key words: Khanya Maths and Science Club, disposition, attitude, after-school enrichment programmes, constructivist-developmental approach, situated learning theory, community of practice, Test of Science Related Attitude (TOSRA).
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- Date Issued: 2016
Management of street traders to boost the local economy in King William's Town (Buffalo City Municipality)
- Authors: Hlope, Sithabiso Patrick
- Date: 2016
- Subjects: Economic policy King William's Town (South Africa) -- Economic policy , Street vendors -- South Africa -- King William's Town
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/13049 , vital:27147
- Description: This study deals with the management of street traders to boost the local economy of King William‟s Town. The purpose of the study is to conduct the research on how street traders can be managed to boost the local economy of King William‟s Town. The intention is to research the challenges they have and how to improve them. To be able to conduct the study, the researcher deemed it necessary to study existing literature on Local Economic Development. The way street traders are managed presently has been investigated and analysed in order to develop recommendations for solving the challenges facing street trading in King William‟s Town. The researcher conducted interviews in the form of face-to-face discussions and scheduled interviews with street traders, municipal authorities, and the iQonce Small Business Association. The literature review and interviews enabled the researcher to develop recommendations to solve the challenges. These recommendations will be helpful to the King William‟s Town municipality. The findings of this study reveal that there are challenges in the manner in which street trading is managed in King William‟s Town. Some of the challenges are a lack of a LED policy, poor infrastructure, failure to implement decisions, lack of financial support for street traders, and underutilisation of the budget. The researcher has made some recommendations in terms of support and assistance for street traders, the formulation of a LED policy, and plans and systems needed to be put in place for the management of street trading in King William‟s Town. The researcher summarises the study by claiming that if the proposed recommendations can be thoroughly implemented, there will be some improvement in the manner in which the street traders are managed.
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- Date Issued: 2016
Designing a proxemic natural user interface to support information sharing among co-located mobile devices
- Authors: Lee Son, Timothy
- Date: 2016
- Subjects: User interfaces (Computer systems) Mobile computing
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10948/12845 , vital:27126
- Description: Existing information sharing methods used by mobile devices require the user to repeat a series of steps to share one or more selected files with another individual, where the entire process is repeated for sharing the same file(s) with multiple individuals. Due to constant advancements in mobile computing, mobile devices are able to provide new, more intuitive, and easier solutions to sharing information. Natural User Interfaces (NUIs) primarily focus on the reuse of existing knowledge (from other applications or activities) or human abilities (such as touch, speech, and gestures) to provide a more accurate and usable solution to existing human computer interaction (HCI) systems. The interaction techniques of NUIs have transformed these human abilities. The main research objective was to design a proxemic NUI to provide an accurate and usable solution to support information sharing among co-located mobile devices. The development of MotionShare supported multiple devices to share information simultaneously using NUI interaction techniques. An initial calibration setup allowed MotionShare to calculate the approximate positions and orientations of every device in the environment. Novel NUI interaction techniques were implemented because of the known positions of these devices. MotionShare was evaluated using two evaluation techniques, namely analytical and experimental. The results showed device positioning to have a mean precision, trueness, and recall of 72.21%, 91.39%, and 71.63% respectively. The results showed MotionShare gestures to have a recall of 90.50% and 100.00% for the point gesture and the touch gesture respectively. The experimental technique consisted of a pilot study (formative evaluation) and a usability evaluation (summative evaluation). The results of the usability evaluation showed high user satisfaction and statistical analysis, which revealed MotionShare to achieve the main research objective. These results also showed that participants preferred the touch gesture to the point gesture, but expressed both gestures can be utilised for the tasks of MotionShare.
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- Date Issued: 2016
Investigating the role of Hsp90 and LRP1 in FN matrix dynamics
- Authors: Boël, Natasha Marie-Eraine
- Date: 2016
- Subjects: Extracellular matrix , Molecular chaperones , Heat shock proteins , Cancer , Fibronectins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/2713 , vital:20319
- Description: Fibronectin (FN), a matrix protein responsible for regulating processes including migration and differentiation, is secreted as a soluble dimer which is assembled into an insoluble extracellular matrix. The dynamics of FN matrix assembly and degradation play a large role in cell migration and invasion contributing to the metastatic potential of cancer cells. Previous studies from our group have shown the direct binding of Hsp90 and FN in vitro and that inhibition of Hsp90 with novobiocin (NOV) caused internalisation of the FN matrix. However, the receptor mediating this internalisation is currently unknown. Low density lipoprotein 1 (LRP1) is a likely candidate as it is a ubiquitous receptor responsible for regulating internalisation of diverse ligands and is known to bind both Hsp90 and FN. We used wild type and knockout LRP1 cell lines to study the endocytosis of FN via this receptor. Here, we demonstrate that LRP1-deficient cells accumulated greatly increased levels of FN and were found to be less sensitive to pharmacological inhibition of Hsp90 by NOV. LRP1-expressing MEF-1 and Hs578T breast cancer cells experienced an increase in total FN in response to NOV, at concentrations below the EC50 value, followed by a dose-dependent loss of FN. We attributed greater FN levels to a loss of extracellular FN matrix coupled with increased internalisation of FN. Cell-surface biotinylation and DOC assays showed that loss of extracellular FN was specific to LRP1-expressing MEF-1 cells. Furthermore, we demonstrate that the loss of extracellular FN is not affected by changes in FN mRNA levels as determined by qRT-PCR, and that treatment with NOV resulted in the accelerated degradation of FN in the presence of cycloheximide. Immunoprecipitation studies reveal a putative complex exists between FN, Hsp90 and LRP1 in both cancer and non-cancer cells which is not perturbed by NOV. Western analyses revealed increased proteolytic processing of LRP1 in response to NOV which we proposed, based on literature, to modulate signalling pathways as a potential mechanism for regulating FN turnover. Moreover, using wound healing assays we identified increased migration to be one of the consequences associated with loss of extracellular FN by Hsp90 inhibition but only in cells containing LRP1. In summary, this study provides new insights into the Hsp90-LRP1 mediated loss of FN matrix and also reveals for the first time the functional consequence related to FN turnover by NOV was an increase in migration in LRP1-expressing cells.
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- Date Issued: 2016
Foundation Phase male student-teachers’ experiences during Teaching Practice: Implications for Initial Teacher Training
- Authors: Makeleni, Sive
- Date: 2016
- Subjects: Student teaching -- South Africa Teachers -- Training of -- South Africa Teaching -- South Africa
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/5171 , vital:29095
- Description: The aim of this study was to explore experiences of Foundation Phase male studentteachers during teaching practice. Four Bachelor of Education Foundation Phase male student-teachers were selected for the purpose of this study. Venturing of males in Foundation Phase specialisation is phenomenon. Foundation Phase has been viewed as a female territory for many years. As a result, there are few males teaching in FP. The study employed the qualitative approach as a suitable approach. As the study sought to explore experiences, a phenomenological design was followed. In a true phenomenological design, unstructured interviews and personal diaries are used for data collection. In this study data were collected through unstructured interviews and personal diaries of the participants. I employed interpretivism as a research paradigm. The study found that despite South Africa being a democratic country Foundation Phase is still viewed as a female space. Findings reveal that male student-teachers feel that in a democratic country, gender equality should be exercised. The study revealed that teaching profession is undermined. As much as participants reported about insecurities, they also reported about the benefits of being a male studentteacher in Foundation Phase specialisation. One of the benefits reported is knowledge and understanding of child development theories. The participants reported that during TP, they were seen as role-models, as father figures and resources. The research recommends that Initial Teacher Education Programme should do more in preparing male student-teachers venturing in Foundation Phase. The research also suggests that all stakeholders involved in education should have a programme focusing on developing teaching profession to be a prestigious profession. The government and all education stakeholders should also focus on gender equality within FP specialisation, both in Initial Teacher Education institutions and in schools.
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- Date Issued: 2016