Assessment of the Department of Rural Development and Agrarian Reform's maize cropping programme in the Eastern Cape
- Authors: Mqamelo, Luvolwethu
- Date: 2017
- Subjects: Cropping systems Crops and climate Crop yields
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9165 , vital:34288
- Description: The Department of Rural Development and Agrarian Reform maize cropping programme is one of the flagship projects that has been entrusted by the provincial government to tackle the social ills faced by the rural communities of the Eastern Cape Province. The programme is in line with a number of policies that strive to unlock the agricultural potential in inducing the much talked about radical economic transformation. This study was aimed at establishing if the outlay of the implementation processes and the outcomes of the maize cropping programme in Eastern Cape are sufficient to achieve the stated objectives. The study adopted a triangulation method to get comprehensive understanding of the programme and for accuracy and verification of the data collected. A pre-tested semistructured questionnaire was used to collect the demographics and socio-economic status of the respondents; technical skills and capabilities; implementation processes of the programme and the perceptions of the participants and key strategic role players in the maize cropping programme. A total of 120 respondents from DRDAR’s 2016/2017 financial year supported list and 9 key informants were sampled for the study. Furthermore, a gross margin analysis was used to establish the viability and the sustainability of the programme with and without DRDAR’s subsidies. For purposes of determining if there was any relation between the maize enterprise income and 13 independent variables, a multiple regression model was employed. The results of the study show that the programme is dominated by old and uneducated individuals with an average age of 60 years. The implementation processes are proving to be fairly progressed however, at a very limited scale. This includes provision of production inputs and mechanization services on time, formation of self-serving legal entities and unlocking the private sector investment. The gross margins generated from the programme have been observed to be non-viable and unsustainable. As a result, only 31% of the respondents were able to attain a gross margin equal or greater than the allocable costs of producing maize both under irrigation and dry land. The results of the regression model indicated that, only 5 of the 13 independent variables (household gender, marital status, years of participation, area planted and yield) were found to have a significant effect to the maize enterprise income. Overall, the DRDAR’s maize cropping programme has failed to meet its intended objectives. In addition, more than 70 percent of the programme participants produced for subsistence purposes and showed no desire to fully commercialise their operations.
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- Date Issued: 2017
Assessment strategy framework for the National Diploma : fashion course at one Eastern Cape Comprehensive University
- Authors: McLaren, Lorian
- Date: 2017
- Subjects: Fashion -- Study and teaching Fashion design -- Study and teaching Clothing trade -- Handbooks, manuals, etc
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10353/4904 , vital:28750
- Description: The purpose of the study was born out of a perceived need to establish an assessment strategy framework for the fashion programme of one Eastern Cape comprehensive university. The study focused on one Eastern Cape comprehensive university. The programme, National Diploma: Fashion, is offered by the university at two campuses (120km apart). Although this programme is currently offered at both of the sites under the auspices of the university, disparity exists in many of the academic functions within the programme. The most challenging is assessment and the implications of a non-existent standard framework for assessment across both campuses. This research undertook to identify a framework that would best serve the Fashion programme of the researched university. Assessment in the context of this study referred to the process of both gathering evidence of student learning as well as assigning grades to that learning. The lack of an assessment framework affects the quality of assessment. Consistency in the assessment process across both campuses is important. At present assessment is not consistent as it is done independently on each campus. This lack of consistency could prove to favour students at the one campus while marginalizing students at the other campus and vice versa. Inconsistency arises from staff having no common assessment framework to refer to when assessment takes place. This study was a case study. Interviews were conducted with a sample of lecturers and students from both sites. A document analysis of relevant policies was done. The documents included the Higher Education Quality Committee document Criteria for Programme Accreditation, 2004, the South African Qualification Authority document Criteria and Guidelines for Assessment of NQF Registered Unit Standards and Qualifications, 2001, and the South African Qualification Authority document Guidelines for Integrated Assessment, 2005. University policy documents pertaining to assessment were also included in the research.The findings of this study lead to the conclusion that there is no clear assessment framework currently in place for the National Diploma: Fashion at one Eastern Cape comprehensive university. The assessment methods currently in use are not fully understood and comprehended by lecturers or students. The assessment types are limited with little or no variety as to how assessment is practiced. Although continuous assessment is advocated in the department, a lack of understanding by lecturers and students as to the true practice of continuous assessment is evident. Much of the assessment is done at the end of a teaching module, rather than embedded in the teaching module. This means that assessment is done of learning rather than for learning.
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- Date Issued: 2017
Attitudes of community stakeholders towards stigmatization of people living with HIV/AIDS in Highland View, linked to St Patrick's Clinic, Bizana, Eastern Cape
- Authors: Nkalane, Athenkosi
- Date: 2017
- Subjects: AIDS (Disease)-- Social aspects -- South Africa -- Eastern Cape HIV-positive persons -- South Africa -- Eastern Cape Stigma (Social psychology)
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/9446 , vital:34352
- Description: The researcher attempted to investigate attitudes of community stakeholders towards stigmatization of people living with HIV/AIDS (PLWHA). This is because people living with HIV/AIDS are usually subjected to negative and positive attitudes in South African communities. The study made use of a case study as a specific research design which was explorative and descriptive. The study employed in-depth interviews, focus group discussions and secondary data sources to collect data. The study was a cross-sectional and adopted a non-probability sampling methodology, but specifically purposive technique. The study used 25 participants who consisted of 12 PLWHA, 5 nurses, and 8 selected community members. The data were analyzed through content thematic analysis. The findings that emanated from the study were: awareness of stigmatizing attitudes subjected to people living with HIV/AIDS (PLWHA); the attitudes displayed towards PLWHA, the drivers of stigma; the impact of stigma on PLWHA; PLWHA suffers immense psychosocial deficit; PLWHA face a state of abject poverty; PLWHA faces Psycho-emotional deficit; the secrecy surrounding the virus forms a fertile ground for infection; fear of contagion; stigma confounds chances of HIV/AIDS disclosure and issues of confidentiality. The study recommended that: the government should prioritize the PLWHA’s human rights; adoption of social media platforms to raise awareness and education of HIV/AIDS; collaboration between the Department of Health and the Department of Traditional affairs; invention of a youth-based psycho-emotional programme; and establishment of in-depth community supported interventions.
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- Date Issued: 2017
Attitutes of professional nurses towards the dimensions of quality care in the community health centres of the King Sabatha Dalindyebo sub-district, Eastern Cape
- Authors: Ntuku, Nonzukiso Sheila
- Date: 2017
- Subjects: Community health services -- South Africa -- Eastern Cape Nursing services -- South Africa-- Eastern Cape
- Language: English
- Type: Thesis , Masters , MCur
- Identifier: http://hdl.handle.net/10353/13989 , vital:39737
- Description: The study focused on attitudes of professional nurses towards the dimensions of quality care. Quality care is the main focus of the Department of Health in the Republic of South Africa. The researcher believes that professional nurses form the backbone of the whole health care fraternity; hence they are central to the study of quality care dimensions. The aim of the study was to determine and describe the attitudes of professional nurses towards the dimensions of quality care in the community health centres of the KingSabatha Dalindyebo sub-district. A quantitative descriptive design was employed to meet the objectives of the study. The non-probability purposive sampling method was used to select participants to provide information on the attitudes of professional nurses towards the dimensions of quality care in the community health centres of the King Sabatha Dalindyebo sub-district, Eastern Cape. From the target of 84 professional nurses who were employed at the five community health centres of King Sabatha Dalindyebo sub-district of the O.R. Tambo District, Eastern Cape Province, 70 (83,3percent) professional nursesresponded to the questionnaires. A self-administered questionnaire written in simple English was used to collect data from the professional nurses regarding their attitudes towards dimensions of quality care. The researcher adhered to the ethical standards of nursing research as described by the Democratic Nursing Organisationof South Africa regarding anonymity, confidentiality, privacy and quality research, according to which the names of professional nurses were not recorded on the questionnaires. The data were analysed using the Statistical Package for the Social Sciences (SPSS) computer software. The distribution of the results has been presented in tables and a bar graph. The results of the study revealed that the professional nurses of these community health centres had positive attitudes towards the dimensions of quality care.
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- Date Issued: 2017
Av evaluation of the contribution of shelters in offering psychological empowerment to survivors of abuse : a study of selected shelters in Pretoria
- Authors: Nyamutinga, Dudzai
- Date: 2017
- Subjects: Women's shelters -- South Africa Abused women -- Services for -- South Africa Victims of family violence
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9209 , vital:34305
- Description: This research explored the contribution made by shelters in the psychosocial empowerment of survivors of abuse in the contemporary South Africa. Abuse of women is increasing at an alarming rate and government is not able to handle the task single-handedly. The study utilized convergent mixed methods approach (Creswell, 2014) with qualitative and quantitative approaches used in tandem; but with the qualitative approach being more dominant, while quantitative approach was less dominant. For research designs, the qualitative approach used a case study design; while the quantitative method utilized a mini survey design. On the unit of analysis, 52 participants participated in the qualitative sample, with the following sample distribution: 30 participants (15 from each shelter) participated using in-depth interviews, 10 participants (5 from each shelter) constituted a focus group discussion with 12 participants (6 from each shelter) interviewed as key informants. The quantitative approach had a sample of 100 samples women who were victims of abuse complete a mini survey based on questionnaire interviews. The study identified many factors that prompted women to go and reside in shelters from physical abuse and sexual abuse usually perpetrated by women’s spouses taking the larger share. Other important drivers included economic and emotional abuse. This resonates with some of the tenets of the critical feminist theory that an offender’s victimization of another person is an expression of domination and control (Brownmiller and Russell, 1975 in Wilcox 2010). Furthermore, the United Nations recognizes that violence against women is a manifestation of historically unequal power relations between men and women, contributing from male domination and discrimination over women. The study also established various psychosocial services provided to survivors of abuse. Such services were important in order to strengthen survivors’ coping capacities, raise their confidence and reinforce their decision-making processes pertaining to their lives. Some of these psychosocial services provided include provision of accommodation, nutritional meals, therapeutic counselling, basic counselling sessions, group work sessions, access to skills development programmes, assistance in maneuvering the judicial system, job placements, parenting skills, access to education and provision of preschool for their children and information and referrals. This study also managed to unearth and identify the various institutional and operational challenges that beset the running of the two selected shelters in Pretoria. Acutely, shelters suffer funding challenges, with funding either being inadequate or having to take so long before it is availed and, therefore, almost affecting the day to day operations of the shelters. These shelters were also challenged by donor regulations, inadequate infrastructure and a continuous increase of survivors and children in their care. Furthermore, the study identified the shelters’ effectiveness to raise survivors’ hope for the future; boost their confidence; improve their social functioning; provide emotional and practical support; offer them the requisite accommodation and meals; and also provided them with different kinds of skills that made them financially independent. The research recommended that a decentralization of shelters to rural areas across all provinces and the nation at large would go a long way in ensuring that all survivors will get the assistance they need. As the rural-urban migration takes its toll, the abuse of women also continues to increase. Funding is the key to the success of every project, and, therefore, the provision of enough and consistent funding and a sustainable plan to the shelters will ensure the smoothness of the provision of services and reduce staff burnout, reduce high staff turnover, and increase the stability of trained personnel. The study also recommends the government to consider subsidizing the salaries for the staff working in shelters so that their salaries would be on par with their colleagues in other organizations. Government should also facilitate the institution of shelters for survivors of abuse as some men feel that the issue of abuse focuses on females and therefore men shy away from accessing the services of the shelters.
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- Date Issued: 2017
Bank Competition and Economic Growth: The Case of South Africa, Russia and China
- Authors: Sokapase ,Zukile
- Date: 2017
- Subjects: Banks and banking
- Language: English
- Type: Thesis , Masters , MCom (Economics)
- Identifier: http://hdl.handle.net/10353/14120 , vital:39846
- Description: This study examined the relationship between bank competition and economic growth across South Africa, Russia and China. In this regard, the study conducted a comparative analysis which allowed for country differences and thus enhanced the provision of relevant policy guidance given how each of the country’s economic growth is reacting to changes in bank competition. To account for country differences, the study estimated a random coefficient panel (RCP) model using Swamy’s generalised least squares (GLS) estimators for the period 1999 – 2015. Across the three countries, bank competition and economic growth have been found to be positively related. In addition, bank competition has been found to be highly significant in influencing economic growth across the three countries. However, the extent of influence that bank competition has on economic growth differs in each country. Therefore, this study recommends that policies aimed at promoting competition in the banking sector of South Africa, Russia and China should be adopted in order to promote economic growth.
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- Date Issued: 2017
Biological children's experiences and perceptions about having foster children in their households: a case study of Zwelitsha township
- Authors: Ntshongwana, Zintle
- Date: 2017
- Subjects: Foster children -- South Africa -- Eastern Cape Social work with teenagers -- South Africa -- Eastern Cape Foster parents -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/3033 , vital:28240
- Description: Most research studies focus on foster children or foster parents, there is dearth of research on the impact of fostering on biological children. This aim of this study was to fill the gap in the literature regarding the experiences of biological children sharing a home with foster children. It also investigated how biological children perceive foster care and challenges encountered by children under foster care. This was achieved through a qualitative research approach. The study was conducted at Zwelitsha Township in King Williams Town. Data was collected through semi-structured interviews with thirty biological children of foster parents and ten social workers in practice. Two focus group discussions were held with twenty foster parents. A focus group discussion guide was used in collecting information during the discussions. Purposive sampling was used in the study and data was analysed according to themes. The major findings revealed that fostering has both positive and negative impacts on biological children and the foster family. Biological children find foster care placement as helpful to children whose parents cannot provide care and protection for children. They gain and learn new knowledge through fostering and are happy about their new siblings in the family since they have companionship. On the other hand, minority of biological children were ambivalent about foster care as they were uncertain between altruistic feelings of making a difference in the lives of needy children against the negative outcomes of fostering. One biological child was negative about foster care. This participant did not like the idea of fostering and did not describe any personal positive outcomes resulting from his fostering experiences. The findings revealed that biological children are unable to voice out their views and ideas regarding foster care placement. The findings also revealed that biological children are not passive observers on how fostering influences their daily lives. Instead, they use strategies to influence fostering processes, especially to protect their foster siblings for the fostering process to be successful, while also having feelings of responsibility for their foster siblings. The findings further suggest that despite the complex nature of the fostering task, professionals should recognise and acknowledge the input of biological children to fostering. It is, therefore, suggested more training should be provided to foster parents to continually include the opinions of their own children in fostering decisions.
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- Date Issued: 2017
Biomass yield, nutrient content and silage quality of three forage sorghum (Sorghum Bicolour (L.) varieties grown in the semi-arid region of Eastern Cape Province, South Africa
- Authors: Gwantshu, Vuyolwethu Hamilton
- Date: 2017
- Subjects: Sorghum -- Varieties -- South Africa -- Eastern Cape Sorghum -- South Africa -- Eastern Cape Forage
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9545 , vital:34368
- Description: The study determined the biomass yield, nutrient content and silage quality of forage sorghum varieties. Three varieties were used (King, PAN 868, and PAN 888), in a randomized complete block design (RCBD) with three blocks and three replicates per treatment at the University of Fort Hare research farm. The research study was conducted during the 2016/2017 growing season. The forages were harvested at flowering stage of growth. Biomass yields of fresh forage and dry matter yield were measured. Sub-samples were then dried, milled and analysed for nutrient content. Furthermore, the forage sorghum was ensiled and the silage was analysed for fermentation parameters and nutrient content. The fresh and dry matter biomass yield showed no significant differences (P>0.05) among the varieties. The highest fresh biomass yields were observed in PAN 888 variety was 13400 kg/ha, King had 12600 kg/ha and PAN 868 had 12600 kg/ha. The dry matter yield observed in PAN 888 was 4300.00 DM kg/ha, PAN 868 had 3633.33 DM kg/ha and King yielded 3533.33 DM kg/ha. The different sorghum varieties showed a significant (P<0.01) difference in some chemical composition parameters namely DM, NDIN and NSC. The different sorghum varieties had significant difference (P<0.01) in the silage fermentation parameters of pH, starch and lactic acid. The sorghum silage varieties were significantly (P<0.01) different in the starch fermentation with values of 0.001percent. The sorghum silage varieties were significant different for fermentation of lactic acid with values ranging from 1.99 to 2.62percent. However, PAN 868 and PAN 888 were not different from each other. Similarly, all varieties were different (P<0.05) in the pH with values ranging from 4.16 to 4.53. There were no significant (P>0.05) differences in CP, Fat, NDF, ADF, Hemicellulose, Ash, and ADIN content of the silages. The mineral composition (macro and micro) of the silage were not significantly (P>0.05) different among the varieties. In conclusion, the dry matter production and nutrient content of the three forage sorghum varieties were not significantly different. The silage fermentation quality showed significant (P<0.05) differences among the three sorghum varieties. Therefore, according to the results of this study PAN 888 is the recommended variety to grow in the semi-arid region of the Eastern Cape.
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- Date Issued: 2017
Blessing or curse? : an evaluation of the African Growth Opportunity Act
- Authors: Matenga, Lloyd
- Date: 2017
- Subjects: United States -- African Growth and Opportunity Act Tariff preferences Terms of trade
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10353/8825 , vital:33667
- Description: The core of the commercial relations between the United States (US) and Sub-Saharan African (SSA) countries is primarily based on the advancement of unilateral preferential treatment as envisaged under the African Growth and Opportunity Act (AGOA). AGOA is a bipartisan US legislation promulgated to govern the North-South agreement between the US and SSA countries. Notably, AGOA can be categorized as an exception to the Most Favored Nation (MFN) clause of the General Agreement on Tariffs and Trade (GATT) and consequently under the legal framework of the World Trade Organisation (WTO). The US through the AGOA Extension and Enhancement Act (TPEA) has prolonged the lifespan of AGOA to 2025 in consonance with AGOA and other relevant US legislation. However, there is an ongoing debate as to whether AGOA is working to the advantage or benefit of the eligible SSA countries. This is due to several problematic issues which inter alia relate to the legitimacy of the unilateral preferential treatment, the applicable Rules of Origin (RoO) and the requirement for adoption of robust intellectual property protection regimes as impediments to the enjoyment of AGOA duty and quota free benefits. This study will thus examine the pertinent legal issues underpinning the granting of unilateral preferential treatment in favour of the SSA countries under AGOA and assess the extent to which AGOA complies with or fulfills the purposes and objects of the Enabling Clause.
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- Date Issued: 2017
Characterisation and cryopreservation of semen from indigenous Namaqua Afrikaner sheep breed, in comparison with Dorper and Dohne Merino breeds
- Authors: Letsoalo, Phutiane Thomas
- Date: 2017
- Subjects: Merino sheep Dorper sheep Semen
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/11707 , vital:39099
- Description: The aim of this study was to characterise and cryopreserve semen of the indigenous Namaqua Afrikaner breed, and to compare it to that of Dorper and Dohne Merino sheep, whose semen is commercially frozen on a large scale. The study was conducted between January and August 2015. September 2013-born Namaqua Afrikaner (12), Dohne Merino (12) and Dorper (9) rams were used in the study. The rams were kept under kraal conditions with adequate shade, and they received a high protein, high energy diet. Originally it was envisaged to collect semen samples using the artificial vagina (AV) method, which proved to be problematic with the Namaqua Afrikaner rams. Semen samples were subsequently collected twice a week by either AV (Dohne Merino and Dorper) or electro-ejaculation (EE; all three breeds). Macroscopic sperm traits were assessed and sperm concentration determined immediately after collection. Each semen sample was diluted with Triladyl® (1:3) and subsequently frozen in liquid nitrogen vapour in straws. Frozen straws were thawed and evaluated at 7, 30 and 90 days after cryopreservation. A droplet (0.5 ml) from each thawed sample was assessed microscopically for post-thaw motility and percentage live sperm. Nigrosin-Eosin smears were prepared of fresh, diluted and frozen-thawed semen samples for determination of percentage live sperm. Data of all the traits were analysed with the GLM and CHI-SQUARE procedures of the SAS statistical package. Breed had a significant effect on ejaculate volume of fresh semen collected either via electro-ejaculation or artificial vagina. Dorper rams (1.37 ± 0.08 ml) and Dohne Merino rams (1.20 ± 0.08 ml) produced ejaculates with higher (P <0.05) semen volume than Namaqua Afrikaner rams (1.09 ± 0.08 ml) when using the EE. With the AV method, Dohne Merino rams (1.46 ± 0.08 ml) produced a higher (P <0.05) ejaculate volume than Dorper rams (1.22 ± 0.08 ml). Motility of the frozen-thawed semen samples was lower than that of the fresh and diluted samples for all breeds and collection methods. Furthermore, motility 3 hours after thawing was also lower than motility assessed immediately after thawing for all breeds and collection methods. Motility of frozen-thawed semen collected with an AV and evaluated at 7, 30 and 90 days after freezing was significantly higher than that collected via EE. Dorper rams had the lowest sperm concentration (1.10 ± 5.29x109 sperm/ml) and the Namaqua rams the highest sperm concentration (1.22 ± 5.20x109 sperm/ml) (P ˂0.05). The AV samples had a higher (P >0.05) sperm concentration (1.20 ± 3.68x109 sperm/ml) than the EE samples (1.11 ± 3.51x109 sperm/ml). The percentage live sperm in the fresh semen samples did not differ among Namaqua Afrikaner (67.76 ± 1.94percent), Dohne Merino (68.59 ± 1.94percent) and Dorper (72.82 ± 1.98percent) rams. The percentage live sperm for all three breeds dropped considerably after freezing to 17.76 ± 2.03percent, 17.86 ± 2.03percent and 22.72 ± 2.07percent respectively. It remained constant for all the breeds from 7 until 90 days after freezing, indicating that length of storage should not have an effect on percentage live sperm for semen collected via AV or EE. Percentage live sperm of the frozen-thawed semen of the Namaqua rams was lower than that of the Dorper rams, indicating that the Namaqua semen collected via EE did not freeze as well as that of the Dorper semen. In conclusion, neither fresh nor frozen-thawed Dorper and Dohne Merino semen collected via EE did differ significantly. Furthermore, except for semen volume, Dorper and Dohne Merino semen collected via AV did not differ significantly whether evaluated as fresh or frozen-thawed semen. However, both Dorper and Dohne Merino rams produced semen with higher motility and a higher percentage live sperm post-thaw when the semen samples were collected via an AV than via EE. From these results it can therefore be postulated that if Namaqua semen were collected via AV it could have a higher post-thaw percentage live sperm than if semen was collected via EE. Advanced further studies are necessary to investigate the reason for the lower post-thaw survival rate of sperm of the Namaqua Afrikaner rams. This is necessary as semen stored in a cryobank for breeding and conservation purposes for this endangered breed has to be of high quality. Such resources as cryobanks are expensive and funds cannot be wasted on preserving inferior samples that could not fertilize an ovum when needed. The low percentage of live sperm obtained with the frozen-thawed samples in this, as well as other studies on indigenous breeds, is an indication that further research is needed into more suitable freezing protocols. It can be concluded that Namaqua Afrikaner semen cannot be frozen successfully for the purpose of storage in a cryobank, when using a freezing protocol based on Triladyl® as extender. Furthermore, any increase in post-thaw survival rate of sperm will be beneficial and it is therefore suggested that all efforts be made to solve the problem of the Namaqua Afrikaner rams that do not want to ejaculate into an artificial vagina.
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- Date Issued: 2017
Characterisation and cryopreservation of semen from indigenous Namaqua Afrikaner sheep breed, in comparison with Dorper and Dohne Merino breeds
- Authors: Letsoalo, Phutiane Thomas
- Date: 2017
- Subjects: Cryopreservation of organs, tissues, etc Merino sheep Dorper sheep
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/4759 , vital:28510
- Description: The aim of this study was to characterise and cryopreserve semen of the indigenous Namaqua Afrikaner breed, and to compare it to that of Dorper and Dohne Merino sheep, whose semen is commercially frozen on a large scale. The study was conducted between January and August 2015. September 2013-born Namaqua Afrikaner (12), Dohne Merino (12) and Dorper (9) rams were used in the study. The rams were kept under kraal conditions with adequate shade, and they received a high protein, high energy diet. Originally it was envisaged to collect semen samples using the artificial vagina (AV) method, which proved to be problematic with the Namaqua Afrikaner rams. Semen samples were subsequently collected twice a week by either AV (Dohne Merino and Dorper) or electro-ejaculation (EE; all three breeds). Macroscopic sperm traits were assessed and sperm concentration determined immediately after collection. Each semen sample was diluted with Triladyl® (1:3) and subsequently frozen in liquid nitrogen vapour in straws. Frozen straws were thawed and evaluated at 7, 30 and 90 days after cryopreservation. A droplet (0.5 ml) from each thawed sample was assessed microscopically for post-thaw motility and percentage live sperm..
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- Date Issued: 2017
Characterisation of antibacterial compounds produced by Pseudomonas spp. isolated from Hogsback wetlands, Eastern Cape, South Africa
- Authors: Obi, Akudo Odochi
- Date: 2017
- Subjects: Bacteriology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15688 , vital:40507
- Description: With microbial pathogens developing resistance against currently existing antimicrobials, there is an earnest need to discover and develop new therapeutic compounds with unique and/or precise modes of action. Natural products have provided the inspiration for most of the active ingredients in medicines and thus are regarded as a potential screening resource lead for discovery of new drugs. Pseudomonads are ubiquitous, Gram-negative, motile bacteria that flourish and inhabit a diversity of environments. Literature has vastly revealed that some members of the genus Pseudomonas are capable of producing a plethora of biologically active metabolites and thus are regarded as “attractive” bacteria for testing as potential sources for new therapeutic compounds. In this regard, this study was undertaken with an aim to isolate and characterize antibacterial compounds produced by Pseudomonas species isolated from aquatic milieu of the Hogsback wetlands, Eastern Cape, South Africa. A total of 12 water samples were aseptically collected from Hogsback wetlands. To ascertain antimicrobial potential of the isolates, 9 test bacterial (sensitive) strains, namely Escherichia coli ATCC 3695, Vibrio fluvialis, Salmonella typhimurium, Salmonella enteritidis, Pseudomonas aeruginosa ATCC 27853, Escherichia coli (2), Listeria ivanovii, Listeria monocytogenes and Enterococcus faecalis ATCC 19433 were used for antimicrobial screening. A total of 500 presumptive Pseudomonas spp. were isolated and screened for the production of antibacterial compound through the Agar-well diffusion method. About 8percent of the presumptive Pseudomonas spp. produced compound with activity against the test bacteria and based on the levels of activity shown, three isolates coded as A53, Y55 and Y138 were selected and identified by 16S rDNA sequence analysis and The Basic Local Alignment Search Tool analysis revealed that the three isolates (A53, Y55 and Y138) belonged to the Pseudomonas genus. Using MEGA6 software, a molecular phylogenetic tree was constructed by Maximum Likelihood Method. The phylogenetic analysis by this method showed that all three isolate codes (A53, Y55 and Y138) formed the same clade with Pseudomonas viridiflava strain P3 (Accession number: AY972186) and Pseudomonas plecoglossicida strain P9 (Accession number: AY972231) at a high bootstrap value of 100percent. Isolate code A53 and Y55 also grouped together at a bootstrap value of 96percent. Factors affecting antibacterial compound production by the three isolates were elucidated. The effect of pH on the three isolates revealed that isolate code A53 and Y55 could produce antibacterial compounds over all pH ranges (pH 4-9), however, antibacterial production was produced optimally at acidic conditions (pH 4) and a less acidic-neutral condition (pH 6) respectively. Isolate Y138 showed optimal production at pH 5. The effect of temperature on all three isolates was studied at different temperatures ranging between 25°C-40°C. Study on effect of temperature variations on antibacterial production revealed that all three isolates are capable of growing and producing antibacterial compounds over all the different temperature ranges, however, maximum/optimum production was observed at temperature of 30°C. In order to establish the role of plasmids in antibacterial production, two curing agents; Sodium dodecyl sulfate (SDS) and Ethidium bromide under different sub-lethal concentrations (1 mg/ml -11mg/ml and 2.5 μg/ml- 125 μg/ml respectively) were used to cure the selected isolates. Plasmid DNA samples of A53, Y55 and Y138, were separated by electrophoresis on a 0.7percent (w/v) agarose. Agarose gel electrophoresis revealed that not all the concentrations used were effective in curing the plasmid. A Quick Load 1 kb Extend DNA ladder marker was used for determining the size of the plasmids. Isolate code A53 and Y138 had a plasmid size of 48.5 kb while isolate Y55 had a plasmid size of approximately 20 kb. Antibacterial activity by agar-well diffusion method after the curing process revealed that antibacterial production was not lost or prevented, but rather increased. Thus, it is suggested that the genes responsible for antibiotic production in this study were chromosomal-encoded and not plasmid-encoded. This study revealed that Hogsback wetlands may represent a previously unexplored environment/source of discovering novel and potent antibacterial compounds.
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- Date Issued: 2017
Characterisation of human pathogenic vibrios and methicillin-resistant staphylococcus aureus (MRSA) from wild and tank cultured dusky kob (agyrosomus japonicus) in the Eastern Cape Province, South Africa
- Authors: Justine, Fri
- Date: 2017
- Subjects: Marine microbiology Marine microbiology--South Africa--Eastern Cape Staphylococcus aureus infections--South Africa--Eastern Cape
- Language: English
- Type: Thesis , Doctoral , Microbiology
- Identifier: http://hdl.handle.net/10353/16047 , vital:40607
- Description: Introduction: Marine finfish including dusky kob (Agyrosomus japonicus), in addition to its high protein value, is bestowed with numerous health benefits including being heart friendly due to high proportion of omega 3 fatty acids. They are therefore used as powerful preventive measures against cardiovaslcular diseases. They are also important in neurological development during gestation and infancy, thus a regular recommendation by nutritionists. However, fish and its environment may be contaminated by bacteria pathogens. Human infections caused by members of the genus Vibrio, and Staphylococcus especially Methicillin-resistant S. aureus (MRSA) are of increased concern worldwide especially with increased Vibrio disease outbreaks and rapid spread of MRSA in the community. Methicillin-resistant Staphylococcus aureus is regarded as an emerging zoonotic agent, therefore marine fish may be considered as a reservoir for infection. The emergence of multi-drug resistant organisms in both farm animals and humans has also been a challenge that hurdles chemotherapy worldwide. Methods: We enumerated the bacteria flora of skin, gill and gut of marine dusky kob (Agyrosomus japonicus) raised in a recirculatory aquaculture system using metagenomic sequencing of the 16S rRNA. We further screened 120 dusky kob fishes (skin, gill and gut) and 80 water samples from two fish farms and Kariega estuary collected over a 10 month period for human pathogenic vibrios, and MRSA using standard procedures. Vibrio species were further subjected to species specific PCRs delineating them into V. cholerae, V. parahaemolyticus, V. vulnificus, and V. fluvialis. This was followed by elucidation of the genotypic virulence determinants. The Reverse Fragment Length Polymorphism (RFLP) of the cytotoxin hemolysin (vvhA) gene of V. vulnificus strains was carried out to determine the associated biotypes. A total of 277 Vibrio strains consisting of 126 V. fluvialis, 45 Vibrio vulnificus, 30 V. parahaemolyticus and 76 vibrios belonging to other species were subjected to susceptibility testing to 15 antibiotics by the disc diffusion method and resistant strains were further evaluated for their genotypic antimicrobial resistant determinants by polymerase chain reaction followed by plasmid profiling. For MRSA screening, we evaluated the reliability of cefoxitin disc diffusion, oxacillin screen agar and BrillianceTM MRSA 2 agar tests, in detecting methicillin resistance from fish (environmental) samples using PCR detection of the mecA as the gold standard. A total of 33 mecA positive S. aureus strains (MRSA) were subjected to PCR detection of enterotoxins (Staphylococcal enterotoxin A-E) and Panton Valentine Leucocidin (PVL) encoding genes. The SCCmec types were also investigated by multiplex PCR targeting genes encoding type I-V. The antibiogram profiles of MRSA strains to 12 antibiotics by the disc diffusion method and to vancomycin by broth micro dilution method were elucidated. Polymerase chain reaction was employed to evaluate the presence of antibiotic resistant genes. The nucleotide sequences of the rpoB gene were analysed to determine mutations that could lead to rifampicin resistance. Results: A broad diversity of pathogenic and non-pathogenic bacterial species of different taxonomic groups was observed with Proteobacteria revealed as the most common bacteria phyla. Fifty one families were identified with Enterobacteriaceae the most frequently detected in all the anatomical sites and the gut showing the highest species diversity. A total of 606 (60.23 percent ) presumptive Vibrio isolates were confirmed by PCR of which V. fluvialis was the most predominant, 193 (31.85 percent ). This was followed by Vibrio vulnificus 74 (12.21 percent ), and V. parahaemolyticus 33 (5.45 percent ). No V. cholerae strain was detected. Only one of the V. parahaemolyticus strains possessed the trh virulent gene with no tdh detected. Virulence factors of V. fluvialis detected were stn (13.5 percent ), hupO (10.4 percent ), and vfpA (1.0 percent ). V. vulnificus biotype 3, formerly restricted to Isreal was identified. Generally, resistance among Vibrio isolates varied considerably with highest resistances recorded to amoxicillin (76.2 percent ), ampicillin (67.5 percent ), erythromycin (38.3 percent ), and doxycycline (35.0 percent ) while higher susceptibilities were revealed by gentamicin (100 percent ), norfloxacin (97.8 percent ), florfenicol (90.3 percent ), tetracycline (87.7 percent ), and chloramphenicol (87.4 percent ). Multiple antibiotic resistance (MAR) (resistance to ≥ 2 classes of antibiotics) was 58.5 percent . The MAR index did not vary significantly between sites (P>0.05) and values of >0.20 were recorded in 40 percent (108/277) of all the isolates. The resistant genes ampC, blaOXA, tetA, tetM, dfr1, sul1, sul2, ermB, nptII and strA were detected in two or more Vibrio isolates while the SXT intergrase was detected only in one isolate. Seven of the 18 extreme drug resistant isolates possessed 1–3 plasmids of 1.5–10 kb range. A total of 321 presumptive Staphylococcus aureus isolates were recovered by culture out of which 202 (62.9 percent ) were identified as S. aureus by PCR. Of these, 33 (16.3 percent ) strains were mecA positive while the rest, 169 (83.7 percent ) were mecA negative. The sensitivity and specificity of MRSA detection by use of cefoxitin disc (30 μg) test was 93.9 percent and 91.7 percent , that for oxacillin screen agar test (6 μg/mL) was 81.8 percent and 92.3 percent while that of BrillianceTM MRSATM 2 agar was 87.9 percent and 94.1 percent respectively. Staphylococcal enterotoxin SEE was detected in 12.1 percent (4/33) of total strains followed by SEA 3.0 percent (1/33), and SEC 3.0 percent (1/33). SEB and SED were absent. Six of the isolates had the PVL gene, giving a prevalence of 18.2 percent . The most frequently detected SCCmec type was SCCmec type III (17/33; 51.5 percent ), closely followed by SCCmec subtype IVc (10/33; 30.3 percent ) and the least were SCCmec types I and II with only 3.0 percent (1/33) each. The rest four (4/33; 12.1 percent ) did not belong to any of the above types and were therefore considered to belong to other subtypes of SCCmec type IV not tested in the study. For antibiogram patterns, 12 (36.4 percent ) strains were resistant to doxycyline, 10 (30.3 percent ) to trimethroprim-sulfamethoxazole, 9 (27.3 percent ) to tetracycline and only 1 (3.0 percent ) strain was resistant to ciprofloxacin. Nineteen (57.6 percent ) of the MRSA strains had vancomycin MIC of ≤ 2 μg/mL, 4 (12.1 percent ) with MIC range of 4-8 μg/mL, and 10 (30.3 percent ) with values ≥ 16 μg/mL. Overall, 27 (91.percent) isolates showed simultaneous resistance to 3 or more antibiotics. The resistant genes tetM, tetA, ermB, BlaZ femA were detected in two or more strains resistant to various antimicrobials. Resistance to rifampicin was not caused by mutations in the rpoB gene. Conclusions: To the best of our knowledge, this study is the first study reporting a broad, and diverse bacteria flora of skin, gill, and gut of the South African dusky kob as well as the first study on human pathogenic vibrios, and MRSA from marine finfish especially those from recirculatory aquaculture systems. Also, our study revealed that Vibrio species with pathogenic potential are present in marine dusky kob and its environs. Dusky kob is contaminated with CA- MRSA and HA- MRSA that may cause difficult to treat infections with regular antibiotics. Some of the MRSA strains contained toxins that can cause food-borne gastroeneteritis. The present study revealed that MRSA and Vibrio species are potential reservoirs for antibiotic resistant genes, which could be disseminated to other bacterial species probably through plasmids, a public health risk.
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- Date Issued: 2017
Characterization of livestock manures biochar and their effect on soil chemical properties and crop growth under glasshouse conditions
- Authors: Dzvene, Admire Rukudzo
- Date: 2017
- Subjects: Biochar Soil fertility
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9863 , vital:35091
- Description: Biochar is being promoted as an amendment to improve soil properties, crop productivity, and carbon sequestration. In Africa, biochar adoption is hindered by production systems which include technology and feedstocks availability. However, little research has been published on the influence of biochar incorporation on soil chemical properties and early crop establishment. The aim of this study was to characterize biochar from cattle, goat, sheep and poultry kraal manures and their effect on soil properties and crop growth. This was guided by the following specific objectives, (i) to explore the mixed-farming system of Raymond Mhlaba Municipality on availability and utilization of livestock kraal manures (ii) to characterize biochar samples produced from cattle, goat, sheep and poultry kraal manures (iii) to determine the nutrient release patterns of biochars amended in a degraded soil (iv) to determine the effectiveness of converting manure to biochar and cattle manure on maize early development and, (v) to determine the effects of biochar type and application rate on early maize development. The farming system was surveyed to identify quantities of livestock manure, its availability and utilization for soil fertility amendment in cropping in the Raymond Mhlaba Municipality. The emerging results across all the villages revealed that, the mean livestock numbers were, 9.24±8.21 sheep, 9.37±8.15 goats, 7.95±7.66 cattle and 9.02±9.47 chickens. The findings revealed that 94 (82.4percent) of the respondents had access to cropland allocations in the form of outfields and homegardens. However, only 55 (48.2percent) of the respondents were currently cropping their fields. Maize was the most common crop grown followed by butternuts and potatoes. The application of manure by the respondents currently cropping their lands was only 40 (35.1percent) and the quantities used ranged from 210-1450 kg ha-1. The results further showed that the estimated total manure production was 2.9 t year-1, 0.82 t year-1, 0.04 t year-1, and 0.8 t year-1 from cattle, goats, chicken, and sheeps respectively. Drum retort method of slow pyrolysis at a temperature of 400°C was used to produce biochar from cattle, goat, sheep and poultry manure feedstocks. The biochar yields were 63percent, 72percent, 61percent and 83percent on a weight basis for the different feedstocks. The chemical properties of the biochar were significantly different from those of the manure from which they were made. Biochars that were high in Ca and K such as poultry manure biochar and sheep manure biochar indicated higher pH and electrical conductivity values. For instance, sheep manure biochar was (8.1 mS cm-1) and poultry manure biochar was (9.2 mS cm-1). The scanning electron microscopy (SEM) revealed that, the biochars had porous structures ranging from 1.23um to 5.23um in diameter which are important for water conductance and holding capacity. The target soil carbon level to determine the effects of biochar soil incorporation was 2percent and the soil had 0.7percent. Therefore, it was treated with four livestock manure biochars at application rates of 0; 53.2 t ha-1 (CMB); 48.1 t ha-1 (GMB); 50.7 t ha-1 (SMB); and 40.2 t ha-1 (PMB) based on their carbon content to supplement the soil carbon difference. The effect on soil pH was such that SMB increased to 6.44, PMB (6.45), CMB (6.54), and GMB (6.53) relative to the control which did not show any changes. An increase was also observed on Olsen P concentrations (mg P kg-1) which varied with biochar treatments: PMB (6.22), GMB (6.37), SMB (6.44) and CMB (6.44) and were significantly higher than the control. Ammonium-N(NH4+) concentrations (mg NH4+-N) were increased in biochar treatments but, no significant differences were obtained with sampling time. SMB released 7.95 mg kg-1, CMB 7.50 mg kg- 1, PMB 7.46 mg kg-1 and GMB 7.05 mg kg-1, compared to the control 3.23 mg kg-1. Maize growth in soil sampled from farmers fields in cultivation and abandoned treated with biochar without application of inorganic fertilizer did not differ with control (soil only) treatments. However, maize growth in soil treated with biochar and inorganic fertilizer was comparative to manure treatments. This resulted in a follow up study to elucidate the effects of biochar alone and was carried out with cattle, goat, sheep and poultry biochars at five application rates (0, 100, 200, 300, and 400 kg C ha-1) applied to a sandy loam and a clayey loam soil of the Oakleaf and Tukulu soil forms respectively. Post-harvest soil pH, electrical conductivity and Olsen P showed improvements in biochar treatments relative to the control. Improvements in the chemical parameters and plant growth increased simultaneously with biochar application rate. Maize growth was not affected by biochar application at different rates.
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- Date Issued: 2017
Characterization of maize biochars, their effects on soils, early maize growth and effectiveness of fertilizers
- Authors: Pinkson, Sibusisiwe
- Date: 2017
- Subjects: Soil fertility Crops and soils Biochar
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9974 , vital:35211
- Description: The use of maize biochar in smallholder cropping systems of the Eastern Cape (EC) Province could be a strategy that helps mitigate a common challenge such as poor soil fertility in maize productivity. The current study investigated the following: a) comparison of maize biochars derived from the drum and pit kiln pyrolytic systems as soil amendments; b) effects of maize biochars on soil acidity and extractable nutrients of three arable soil types of the EC Province; c) effects of maize biochars on early maize growth and nutrient uptake; d) effect of maize biochars on the effectiveness of organic and inorganic fertilizers for improved soil quality and maize growth. Maize biochars were produced from two pyrolytic systems namely the pit and drum kiln, each maize biochar type was analyzed with respect to its chemical and physical characteristics to investigate its potential as a soil amendment. Both pyrolytic systems were monitored to identify optimum pyrolytic temperatures reached by each process. Observed peak pyrolytic temperature were 300 °C and 500 °C for the pit and drum pyrolytic systems respectively. Both maize biochar types were alkaline in nature with pH values of 8.36 (H2O) and 7.45 (KCl) and 8.95 (H2O) and 7.82 (KCl) for pit and drum biochar respectively. Electrical conductivity (Ec) of drum biochar was 199 mS /m and that of pit biochar 186 mS /m. Generally, there were no differences in the chemical compositions of the two biochar types. However, in most analysis drum biochar gave higher values in nutrient composition compared to the pit biochar. Moreover, according to SEM analysis both biochar materials consisted of an increase in pore size distribution compared to the original feedstock material. In addition, an increase in nutrient composition in the two biochar types compared to the original feedstock was also observed. In an incubation experiment, potential effects of the two maize biochar types derived from the pit and drum kiln pyrolytic systems on soil acidity and nutrients were investigated on three arable soil types of the EC Province. Three soil types investigated were obtained from the University of Fort Hare (UFH), Phandulwazi (PND) and Msobomvu (MSV). The UFH soil was a sandy loam of the Oakleaf form or Eutric Cambisol, the PND soil was a sandy loam over sandy clay a Haplic Luvisol, while the MSV soil was a clay loam of Westleigh form a Ferric luvisol. Both maize biochar types significantly (p< 0.05) increased soil pH of the three soil types in the incubation study from an average of 4.68- 7.5. Soil pH improvement was in the following order pit biochar> drum biochar> control. Also, the two maize biochar types significantly (p< 0.05) increased the release of cations namely Ca, Mg and Na however, some decrease in nutrient concentrations were observed due to increase in pH levels at the later stage of the study. Biochar incorporation increased extractable P release from 30- 40 mg P/kg to an average range of between 120- 160 mg P/kg across all soil types. A glasshouse study was also conducted investigating the effect of different biochar rates of the two maize biochar types used in the previous studies on early maize growth and nutrient uptake, same soil types were used as those in the incubation study. Biochar was applied at application rates of 0, 100, 200 and 300 kg C/ha of biochar. The glasshouse experiment consisted of two cycles, each with a six week period of monitoring maize seedling growth development. Generally, a significant increase in maize growth parameters such as plant height, stem girth, number of leaves and leaf area increased after biochar additions compared to the control. Both biochars significantly improved maize seedling growth at an application rate of 200 kg C/haHowever, the two biochar types displayed varying impacts on maize growth due to influence of soil type and biochar application rates. Nutrient- uptake of Ca, P, Na and Mg showed to improve at an application rate of 200 kg C/ha for both biochar types showing a 50 percent increase compared to the control. Nutrient- uptake patterns for P, Ca, Mg and Na differed across all soil types, mainly due to changes in pH and Na levels which were influenced by increased biochar concentrations resulting in nutrient deficiency. Another glasshouse experiment aimed at investigating the effects on the two maize biochar types on influencing the effectiveness of both organic and inorganic fertilizers for improved early maize growth and soil productivity. Biochar was applied at an application rate of 400 kg C/ha of biochar, fertilizer was applied at application rates of 0, 10, 20, 30 and 40 kg N/ha and manure at 0, 10, 20, 30 and 40 t/ha respectively. The glasshouse experiment consisted of two cycles, each with a six week period of monitoring maize seedling growth development. In the 1st cycle, manure rate of 40 t/ha resulted in the highest plant height of maize seedlings grown in the glasshouse, biochar type also showed to influence plant height. After the 1st harvest Mg, K and P levels improved at 30 t/ha, Na at 20 t/ha and Ca 40 t/ha of manure. Plant height and stem girth improved at 30 kg N/ha of fertilizer, however, nutrient levels for Ca, K and Mg decreased after the 1st harvest, while Na levels increased. Nutrient uptake was the highest for Mg and Na at 40 kg N/ha while for Ca at 30 kg N/ha of fertilizer. During the 2nd cycle plant height was the highest at 30 t/ha of manure, and effect of manure rate was significant (p<0.05) for number of leaves, plant height and biomass. Whereas, manure rate and biochar type effect were insignificant (p>0.05) for pH, Ec and nutrient uptake changes. Maize seedling showed significant response to fertilizer 40 kg N/ha for most of the growth parameters measured (P<0.05), except for germination percentage. All treatments investigated had no effect on germination percentage. The two biochar types showed significant (p<0.05) effects on soil pH and Ec. Drum biochar was slightly better performing than pit biochar. Results obtained from the various studies suggests that maize biochars derived from the pit and drum pyrolytic systems are potential soil amendments in correcting soil acidity and nutrient deficiency in the EC Province. Both biochar types have shown abilities to improve early maize growth and effectiveness of fertilizers in maize based systems of the EC Province. However, some variations in soil fertility and early maize growth were observed as a result of the soil type response towards the two biochar applications. This could also have risen as a result of a number of factors such as the nutritional composition of biochar material, initial soil type chemical composition, biochar and fertilizer application rates and time taken or period to complete each study. Therefore, further experiments should be conducted to validate these results, preferable having longer periods of study, moreover, field experiments. In addition, investigations on potential feedstock materials such as animal wastes used as biochar in the EC Province can be conducted.
- Full Text:
- Date Issued: 2017
Characterization of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) in selected recreational marine waters and beach sand in the Eastern Cape Province, South Africa
- Authors: Ankabi, Olufemi Emmanuel
- Date: 2017
- Subjects: Marine microbiology -- South Africa -- Eastern Cape Staphylococcus aureus infections -- South Africa -- Eastern Cape Pathogenic microorganisms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4992 , vital:28915
- Description: Staphylococcus aureus is a Gram-positive bacterium predominantly found on human skin and in nasal passages with 20 to 40 percent of the population carrying this organism. Although S. aureus is an unspectacular, non-motile coccoid bacterium, it is a perilous human pathogen associated with both nosocomial and community-acquired infections and it is increasingly becoming virulent and resistant to most antibiotics. It is responsible for several infections such as osteomyelitis, toxin-mediated diseases and bacteraemia, with severe infections arising from strains harbouring antibiotic resistance genes together with virulence genes. S. aureus has been largely confined to hospitals and long-term care facilities, but it is now emerging in the community in places such as recreational beach waters, and occurring in healthy individuals with no associated risk factors. This organism has been reported to be released by swimmers in beaches, suggesting that recreational waters are a potential source of community-acquired S. aureus infections. It is possibly the pathogen of greatest concern due to its intrinsic virulence, its capacity to cause various life-threatening infections, and its ability to adapt to varying environmental conditions. This study was aimed at characterizing S. aureus and methicillin-resistant S. aureus (MRSA) in Port Elizabeth, Port Alfred, Kenton-on-sea and East London beaches of the Eastern Cape Province of South Africa. This was done by investigating the occurrence, antibiotic susceptibilities, antibiotic-resistant genes and virulence genes profiles of S. aureus in the selected beaches. To achieve this aim, 249 beach sand and water samples were obtained from the beaches during the period of April 2015 to April 2016. Physico-chemical parameters of beach water was investigated on site using a multi-parameter ion specific meter during sample collection. Samples were filtered and inoculated on m-Endo agar, m-FC agar and bile aesculin azide agar for total and faecal coliform as well as Enterococci respectively. For isolation of S. aureus and MRSA, samples were cultured on Mannitol salt agar and Staph 24 agar. S. aureus was identified using morphological, Gram staining and molecular (PCR) methods. The isolates were further characterized by determining their antimicrobial resistance profiles, antibiotic resistant genes (mecA, rpoB, blaZ, ermB and tetK genes) and detection of virulent genes encoding intracellular adhesion (icaA), enterotoxin (seaA) and cytolytic toxins (PVL). The majority of study sites passed the directives of physico-chemical standards levels set by WHO during the study period. A total of 143 presumptive isolates were obtained of which 30 (30 percent) were confirmed as S. aureus with 22 (73.3 percent) of these confirmed isolates from marine water and 8 (26.7 percent) from marine sand. Upon culturing on MRSA 2 agar, 15 (50 percent) of isolates showed phonotypic resistance to methicillin. Based on Antimicrobial susceptibility tests, (22/30) 73.3 percent of the isolates showed phonotypic resistance to oxacillin. Out of the 30 isolates, 16 (53.3 percent) were mecA positive and were considered methicillin-resistant S. aureus. S. aureus showed high susceptibility to gentamycin, cefoxithin, levofloxacin, ciprofloxacin, imipenem, and chloramphenicol. A large proportion (36.67 percent to 96.7 percent) of the S. aureus isolates was resistant to penicillin G, ampicillin, oxacillin, tetracycline, clindamycin, rifampicin, vancomycin, sulfamethoxazole-Trimethoprim and erythromycin. Multiple antibiotic resistance (MAR) phenotypes were generated from 7 S. aureus isolates showing resistance to three or more antibiotics. The mecA, rpoB, blaZ, ermB and tetM genes coding for methicillin, rifampicin, βeta-lactam, erythromycin and tetracycline antibiotics resistance was detected in 5 (22.7 percent), 11 (45.8 percent), 16 (55.2 percent), 15 (71.4 percent) and 8 (72.7 percent) respectively. The PVL, icaA and seaA genes coding for virulent determinants were detected in 50, 20 and 13.3 percent of the confirmed isolates respectively. Physico-chemical and faecal indicator bacteria results obtained from this study can assist municipal authorities in developing appropriate management strategies for beaches in the study area. The findings of this study showed that the investigated beaches were contaminated with toxigenic and multi-drug resistant S. aureus strains. This emphasizes the need for the implementation of better control measures to reduce the occurrence of antibiotic resistant S. aureus and of virulent S. aureus strains in recreational waters. In our study it was established that the potential of recreational waters to be reservoirs of S. aureus should not be taken for granted, and it is important that beach goers be educated about this organism as well as other related pathogens that could affect human health, especially immuno-compromised individuals. The community should be educated on antibiotic stewardship and the detrimental effects of antibiotics abuse.
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- Date Issued: 2017
Choice of market channels by smallholder vegetable farmers in King Sabata Dalindyebo Municipality in the Eastern Cape province of South Africa
- Authors: Yokwana, Aphelele Lucia
- Date: 2017
- Subjects: Vegetables -- Marketing Farms, Small -- South Africa -- Eastern Cape Vegetable trade -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MAgric
- Identifier: http://hdl.handle.net/10353/11906 , vital:39117
- Description: The study investigates the choice of market channels by smallholder vegetable farmers in King Sabatha Dalindyebo Municipality, in the Eastern Cape Province of South Africa. Vegetables as a group of horticultural crops are important for their contribution as an income support to a large proportion of the rural households. However, enhancing vegetable farmers to reach markets and actively engage in the markets is a key challenge influencing vegetable production in South Africa. The perishable nature of vegetables demands effective marketing channels. The main objective of the study was to investigate factors affecting vegetable farmers’ choice of market channels in King Sabata Dalindyebo Municipality in the Eastern Cape Province. The study adopted a multistage stratified sampling method. Multi-stage sampling was done in which the first stage involved selecting respondents from the different wards in the King Sabata Dalindyebo Municipality. This was done through stratification by separating vegetable smallholder farmers and homesteads within that area (different villages within the area). This was followed by employing quota sampling through the different households and smallholder farmers within those areas in order to determine households and smallholder farmers that are able to provide the needed information concerning the choice of market channels. By stratified random sampling based on village, project membership and smallholder farmers, a sample of 110 heads of households was chosen for the study in eight different wards of KSD. The sampling for this study was based on a large sampling technique of n ≥ 30 as there is no information regarding the population of the total number of homesteads and smallholder farmers that are under each traditional leader (chief) in these study sites. This sample comprises homesteads and smallholder farmers that took part in vegetable production. A structured questionnaire together with field observations and measurements were adopted for obtaining information from household respondents. Descriptive statistics (percentages, means, frequency tables and figures) and a Multinomial logistic regression model have been used to analyze the data. From the Multinomial logistic regression results, farming experience is positively related to choice of farm gate market channel at 1percent level of significance. The age of vegetable farmers was positively related to the choice of direct to consumer market channel at 5percent significance level. The level of education of the vegetable farmers was positively related to the choice of direct to consumer market channel at 5percent significance level. Moreover, the results also showed that the inputs used are positively related to the choice of farm-gate as well as direct to consumer market channels at 5percent significance level. Similarly, means of transportation used and choice of marketing channel of the vegetable farmers are positively related at 1percent significance level at the direct to consumer choice of market channel. Furthermore, access to extension services indicated a positive relationship to the choice of market channel amongst the smallholder vegetable farmers for the direct to consumer at 5percent level of significance which is insignificant for that of the farm-gate. There is therefore, need for strong extension support in assisting the farmers to diversify their production, provide market information thereby enhancing production and opening up channels for market accessibility. This is seen to enhance rural households’ livelihood outcomes in agricultural production thereby improving the choice of market channels by smallholder vegetable farmers and alleviating poverty and improving food security. More so, the government and research institutes need to organize workshops and extension programs in famer’ training for more efficiency in their vegetable production and marketing.
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- Date Issued: 2017
Climate change mitigation and resilience by four major supermarkets in East London, South Africa
- Authors: Sibanda, Patience
- Date: 2017
- Subjects: Climatic changes -- Economic aspects -- South Africa -- Eastern Cape Climate change mitigation -- South Africa -- Eastern Cape Agriculture -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5406 , vital:29231
- Description: Climate change is a human development challenge. Its negative impacts have the potential to reverse the human development gains made in Africa. South Africa, just like many other countries in the Global South, is being negatively affected by climate change. The country`s economy is largely dependent on agriculture, extractive industries and manufacturing, all of which are vulnerable to climate change in different ways. This vulnerability means different public and private stakeholders must institute sustainable climate change mitigation and adaptation measures so as to ameliorate climate change impacts. Gradually transforming to an environmentally friendly ‘green economy’ and the reduction of greenhouse gas emissions are some of South Africa’s climate change mitigation priorities. Just like the public sector, the private sector has a major role to play in this transition to a low carbon economy through minimising its carbon emissions in their operations. Against this background, this qualitative research examines the role played by four major supermarkets (Shoprite, Spar, Pick n Pay and Woolworths), in climate change mitigation and resilience building. It does so through an appraisal of their mitigation and resilience programs. The four supermarkets were purposively selected in East London, Eastern Cape. Data collection combined heavy reliance on desktop discourse analysis with field interviews in the form of purposively sampled key informant interviews. The results show that the four major supermarkets are implementing a variety of climate change mitigation and resilience strategies. These include reduction of greenhouse gas emissions, prioritisation of renewable energy, recycling and environmentally friendly packaging, promotion of sustainable ways of farming and improved fuel efficiency in their transport systems. In terms of policy improvement, the study recommends that there is need for supermarkets to learn from each other’s’ climate change mitigation initiatives so as to advance South Africa’s ‘green economy’ agenda.
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- Date Issued: 2017
Commerce powered by 'National culture'? : an assessment of "Wear Local" campaigns as tools for reinvigorating the textile and clothing industries in Ghana and South Africa
- Authors: Darku, Esther Naa Dodua
- Date: 2017
- Subjects: Textile industry -- Economic aspects -- Ghana Textile industry -- Economic aspects -- South Africa Sustainable development -- Ghana , Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2172 , vital:27619
- Description: This study examines the use of ‘cultural’ imagery and messaging as a tool to revitalise crucial national industries. Specifically, it examines the use of Wear Local campaigns in Ghana and South Africa as strategies to rejuvenate their textile industries and to make them viable in an increasingly competitive global market. Conceptualising Wear Local campaigns as possessing both cultural and economic imperatives, this study highlights how both factors contribute to making products of Buy Local campaigns marketable by showing their importance as both cultural and economic products. Using a descriptive-evaluative design, the study adopted a triangulated research approach comprising a survey, key informant interviews and document analysis. Survey questionnaires were administered to a total sample of 308 respondents in Ghana and South Africa. The qualitative phase of the study involved 10 key informant interviews (comprising textile labour unions, clothing designers, and government officials in both countries) and document/documentary research. The quantitative data were analysed using descriptive and inferential statistics, while the qualitative data were analysed using interpretive approaches, such as content analysis. The results indicate significant uses of national cultural elements in the campaign messaging in both Ghana and South Africa, as well as notable differences in the ways in which these campaigns resonated with consumers in the two countries. For instance, cultural differences accounted for high popularity of the campaign in Ghana and low popularity in South Africa. Following from these findings, the study concludes that the discourse on Buy Local and Wear Local, and the use of national culture in commerce, must go beyond the question of efficacy to examine the conditions under which these campaigns can become an effective economic/market tool. The study makes an important contribution to the existing knowledge on national culture, national economy and globalisation.
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- Date Issued: 2017
Community attitudes on differences between therapeutic interventions offered by traditional healers and biomedics in combatting HIV/AIDS. The case of Dimbaza township
- Authors: Duda, Bathandwa Zimkhitha
- Date: 2017
- Subjects: Healers -- South Africa -- Eastern Cape Traditional medicine -- South Africa -- Eastern Cape AIDS (Disease)-- Treatment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/12788 , vital:39361
- Description: The researcher attempted to investigate and assess the attitudes about therapeutic interventions of traditional healers and bio medics which throw PLWHA into confusion in regards to their health. People living with the virus tend to use both therapies in tandem, yet the rate of HIV/AIDS is escalating in South Africa. This study sought to explore the community attitudes about traditional healing and bio- medics in treating HIV/AIDS. The study used a qualitative approach with explorative and descriptive in designs and a case study as the specific research design. It was also guided by a qualitative research paradigm. For data collection, the study employed in-depth interviews, focus groups and document analysis. The study used non-probability methodology of sample selection, but specifically, snow ball sampling was used to reach to twenty (20) participants; who comprised 6 community members, 6 people living with HIV/AIDS, 4 nurses and 4 traditional healers. Data was analysed qualitatively through content thematic analysis. The study came up with the following findings: lack of faith in traditional healers and their therapies; traditional healers put the PLWHA’s health at stake; cultural beliefs and faith systems’ conformity prompts PLWHA to use healer’s concoctions; ARVs passes the litmus test as a perfect medication in treating PLWHA; ARVs administration fosters good behaviour change; the side effects of the use of ARVs usher in an opportunity to spread the disease; health care workers are source of inspiration in combating stigma; traditional healers’ therapeutic modalities are dangerous to the health of the PLWHA; traditional healers hoodwink societies that HIV/AIDS is a spiritual disease and use of traditional medication in treatment of various ailments supported. The study recommended the following; Scientific researchers need to shed more light on the concurrent use of traditional and bio-medical therapy in managing HIV; World Health Organization need to develop campaigns on the use of African and Western therapies in treating HIV/AIDS; Ongoing training of traditional healers regarding health care practise need to be enforced; Promote cultural methods as a vehicle for prevention strategies to prevent and manage HIV/AIDS and referral of clients to the hospital by traditional healers. Recommendations for social workers; Community based campaigns in managing and preventing the spread of HIV/AIDS and Empower, educate and monitor traditional healers.
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- Date Issued: 2017