- Title
- Incidence and molecular characterization of Hepatitis E virus from swine herds in the Eastern Cape province, South Africa
- Creator
- Adelabu, Olusesan Adeyemi
- Subject
- Hepatitis -- South Africa -- Eastern Cape Virology -- South Africa -- Eastern Cape
- Date Issued
- 2016
- Date
- 2016
- Type
- Thesis
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10353/13966
- Identifier
- vital:39734
- Description
- Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented and the swine HEV identified to date in different countries belongs to either genotype 3 or 4. It has been reported to cross species barrier and cause infections in humans. Hence, Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, genotypes 1 and 2 are exclusively found in human population. However, little data are available regarding the occurrence of HEV in South Africa, especially in the Eastern Cape Province. Hence, this study was designed to assess the prevalence of Hepatitis E Virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province and screened for the presence of HEV. Faecal samples were collected, using sterile swabs, from swine between the age of 2 to 6 months old, placed on ice and transported to the laboratory for further analysis. Viral RNA extraction was carried out followed by nested reverse-transcriptase polymerase chain reaction was used for amplification, after which the amplified products were examined by electrophoresis and viewed under ultraviolet illumination. The positive PCR products were sent for sequencing. Of the 160 faecal samples screened, only seven were positive (4.4percent) for HEV. The nucleotide sequence analyses revealed isolates UFT 01-03 sharing 82percent to 99percent identities with other strains with accession numbers KX896664, KX896665 and KX896666 and UMCF 01-04 with KX896667, KX896668, KX896669 and KX896670 from different regions of the world Phylogenetic analysis also showed that all seven isolates from this study clustered with both human and swine HEV from different geographical regions of the world especially with Japan Human and swine strains, Netherland swine HEV strains (AY032758, AF332620) and human HEV strain from France (JQ763611). We conclude that HEV is present among swine in the Eastern Cape Province, albeit in low incidence, and this does have public health implications. There is need for maintenance of high hygienic standards in order to prevent human infections through swine faecal materials and appropriate cooking of pork is highly advised.
- Format
- 70 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | MSc (Microbiology) ADELABU, AO.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |