Evaluation of the in vitro differential protein adsorption patterns of didanosine-loaded nanostructured lipid carriers (NLCs) for potential targeting to the brain

Kasongo, Kasongo W; Jansch, Mirko; Müller, Rainer H

Title: Evaluation of the in vitro differential protein adsorption patterns of didanosine-loaded nanostructured lipid carriers (NLCs) for potential targeting to the brain
Creator: Kasongo, Kasongo W
Creator: Jansch, Mirko
Creator: Müller, Rainer H
Creator: Walker, Roderick B
Subject: To be catalogued
Date Issued: 2011
Date: 2011
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/184035
Identifier: vital:44160
Identifier: xlink:href="https://doi.org/10.3109/08982104.2010.539186"
Description: The preferential in vitro adsorption of apolipoprotein E (Apo E) onto the surface of colloidal drug carriers may be used as a strategy to evaluate the in vivo potential for such systems to transport drugs to the brain. The aim of this research was to investigate the in vitro protein adsorption patterns of didanosine-loaded nanostructured lipid carriers (DDI-NLCs), using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), in order to establish the potential for NLCs to deliver DDI to the brain. NLC formulations were manufactured using high-pressure homogenization using a lipid matrix consisting of a mixture of Precirol® ATO 5 and Transcutol® HP. The 2-D PAGE analysis revealed that NLCs in formulations stabilized using Solutol® HS 15 alone or with a ternary surfactant system consisting of Solutol® HS 15, Tween® 80, and Lutrol® F68, preferentially adsorbed proteins, such as Apo E. Particles stabilized with Tween® 80 and Lutrol® F68 did not adsorb Apo E in these studies, which could be related to the relatively large particle size and hence small surface area observed for these NLCs. These findings have revealed that DDI-loaded NLCs may have the potential to deliver DDI to the brain in vivo and, in addition, to Tween® 80, which has already been shown to have the ability to facilitate the targeting of colloidal drug delivery systems to the brain. Solutol® HS 15–stabilized nanoparticles may also achieve a similar purpose.
Format: computer
Format: online resource
Format: application/pdf
Format: 1 online resource (9 pages)
Format: pdf
Publisher: Taylor and Francis Online
Language: English
Relation: Journal of Liposome Research
Relation: Kasongo, K.W., Jansch, M., Müller, R.H. and Walker, R.B., 2011. Evaluation of the in vitro differential protein adsorption patterns of didanosine-loaded nanostructured lipid carriers (NLCs) for potential targeting to the brain. Journal of liposome research, 21(3), pp.245-254
Relation: Journal of Liposome Research volume 21 number 3 p.245 2011 1532-2394
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Taylor and Francis Online Terms and Conditions Statement (https://www.tandfonline.com/terms-and-conditions)
Rights: Closed Access

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Walker, Roderick Bryan (Prof)

RU Department of Pharmacy

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