- Title
- Sensitive high-performance liquid chromatographic determination of cyclizine and its demethylated metabolite, norcyclizine, in biological fluids using coulometric detection
- Creator
- Walker, Roderick B
- Creator
- Kanfer, Isadore
- Date Issued
- 1995
- Date
- 1995
- Type
- text
- Type
- Article
- Identifier
- vital:6452
- Identifier
- http://hdl.handle.net/10962/d1006640
- Identifier
- http://dx.doi.org/10.1016/0378-4347(95)00202-T
- Description
- An accurate, sensitive, selective and reproducible high-performance liquid chromatographic method with coulometric detection for the determination of cyclizine and its inactive demethylated metabolite, norcyclizine, in biological fluids has been developed. The drugs were separated using a custom packed reversed-phase C18 analytical column and phosphate buffer (0.05 M, pH 3)-acetonitrile (7:3) as mobile phase. The dual electrode coulometric detector was operated in the "oxidative-screen" mode with the upstream electrode (detector 1) set at 0.55 V and the downstream electrode (detector 2) set at 0.90 V. Serum and urine samples were prepared for analysis by solid-phase extraction, followed by a simple phase-separation step. The limit of quantitation was 1 ng/ml for both cyclizine and norcyclizine in serum and urine.
- Format
- 6 pages
- Format
- Language
- English
- Relation
- Walker, R.B. and Kanfer, I. (1995) Sensitive high-performance liquid chromatographic determination of cyclizine and its demethylated metabolite, norcyclizine, in biological fluids using coulometric detection. Journal of Chromatography B: Biomedical Applications, 672 (1). pp. 172-177. Available: http://dx.doi.org/10.1016/0378-4347(95)00202-T
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Walker, Roderick Bryan (Prof)
Kanfer, Isadore (Prof)
RU Department of Pharmacy
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