- Title
- The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in biological fluids by high pressure liquid chromatography
- Creator
- Gochin, Rosa
- Creator
- Kanfer, Isadore
- Creator
- Haigh, John M
- Date Issued
- 1981
- Date
- 1981
- Type
- text
- Type
- Article
- Identifier
- vital:6364
- Identifier
- http://hdl.handle.net/10962/d1006064
- Description
- The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in serum and urine by high-performance liquid chromatography using sulphafurazole as internal standard is described. The separation was achieved on a reversed-phase column employing acetic acid-methanol as the mobile phase with spectrophotometric detection at 230 nm. Precise simultaneous quantitative analysis of the relative components has been achieved at levels of 0.1 μg/ml for both sulphamethoxazole and its N4-acetyl metabolite using 1 ml of serum or urine.
- Format
- 7 pages
- Language
- English
- Relation
- Gochin, R. and Kanfer, I. and Haigh, J.M. (1981) The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in biological fluids by high pressure liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 223 (1). pp. 139-145. Available: http://dx.doi.org/10.1016/S0378-4347(00)80076-6
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Kanfer, Isadore (Prof)
RU Department of Pharmacy
Haigh, JM (Prof)
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