- Title
- Correlations between the occurence of virulent helicobacter pylori strains and chronic disease conditions in dyspeptic patients hospitalized in Chris Hani Baragwanath Hospital, Gauteng Province, South Africa
- Creator
- Mzukwa, Asisipho
- Subject
- Helicobacter pylori infections Helicobacter pylori Bacterial diseases
- Date Issued
- 2018
- Date
- 2018
- Type
- Thesis
- Type
- Masters
- Type
- Microbiology
- Identifier
- http://hdl.handle.net/10353/10885
- Identifier
- vital:35905
- Description
- Introduction: Individuals predisposed to different chronic disease conditions such as type 2 diabetes mellitus (T2DM), hypertension (HPT) and human immunodeficiency virus (HIV) might be susceptible to persistent H. pylori colonization, which overtime encourages infection with virulent strains. The presence of different types of H. pylori virulence factors especially cagA, dupA and various combinations of vacA subtypes (s1/m1, s2/m1 and s2m2) confer a higher risk of gastric disease development. Aim: This study seeks to recover H. pylori from gastric biopsy specimen from patients with different chronic disease conditions referred to the GIT unit at Chris Hani Baragwanath Academic Hospital, Soweto, and confirm them and further determine the presence of virulent H. pylori strains in patients with T2DM, HPT and HIV. Methods: Gastric biopsies were obtained from 101 patients with different chronic disease conditions after obtaining consent between August 2017 and February 2018. H. pylori was isolated on selective Colombia blood agar. Presumptive isolates were identified following standard microbiology and biochemical technique. These isolates were confirmed by polymerase chain reaction (PCR) targeting the glmM gene. The H. pylori positive samples were further screened for cagA, dupA and vacA genotypes. Results: H. pylori was recovered from 26 patients, of which 8 were patients with T2DM, 10 with HPT and 8 with HIV. There was no correlation between H. pylori infection and the chronic conditions, but the majority of H. pylori positive participant carried virulent H. pylori strains. The cagA and the dupA genes were more prevalent in T2DM and HPT patients, however the association between these genes and the 2 chronic conditions was not significant. The vacA subtypes common in hypertensive patients compared to the other conditions were the s1m1, s1m2, and s2m2 and the association between these vacA forms and hypertension was significant. Majority of HIV infected patients were found to carry non-virulent H. pylori strains. Conclusion: The prevalence of H. pylori among the general population of patients with T2DM, HPT and HIV was low. Although infected patients are found to be carrying virulent strains of H. pylori, there is no significant association established between the virulent strains and the chronic conditions. Furthermore, HIV-infected patients were found to be carrying non-virulent strains of H. pylori.
- Format
- 66 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | MSc (Microbiology) MZUKWA, A Oct Grad 2018.pdf | 2 MB | Adobe Acrobat PDF | View Details Download |