- Title
- In vitro analysis of putative cancer stem cell populations and chemosensitivity in the SW480 and SW620 colon cancer metastasis model:
- Creator
- Slater, Cindy
- Creator
- de la Mare, Jo-Anne
- Creator
- Edkins, Adrienne L
- Date Issued
- 2018
- Date
- 2018
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/164401
- Identifier
- vital:41115
- Identifier
- DOI: 10.3892/ol.2018.8431
- Description
- The cancer stem cell (CSC) theory implicates a small subpopulation of cells with stem like properties, which is responsible for tumour initiation, development and metastasis. The unique biological and functional characteristics of CSCs, widely associated with treatment resistance, indicate an association between metastasis and stemness. It was hypothesised that metastatic cell lines may be enriched in CSCs and that this would correlate with a more resistant tumour. In the present study, the SW480 and SW620 paired cell lines derived from a colon adenocarcinoma and its lymph node metastasis, respectively were compared as an in vitro model of cancer progression. Their chemosensitivity and CSC properties were investigated. A range of in vitro assays were performed, including the side population assay, ALDEFLUOR assay, tumoursphere assay and assessment of CSC associated surface phenotypes.
- Format
- 12 pages
- Format
- Language
- English
- Relation
- Oncology letters
- Relation
- Slater, C., de La Mare, J.A. and Edkins, A.L., 2018. In vitro analysis of putative cancer stem cell populations and chemosensitivity in the SW480 and SW620 colon cancer metastasis model. Oncology letters, 15(6), pp.8516-8526
- Relation
- Oncology letters volume 15 number 6 1 11 April 2018 1792-1082
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Spandidos Publications Statement (https://www.spandidos-publications.com/pages/about)
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RU DST/NRF SARChI for Molecular and Cellular Biology of the Eukaryotic Stress Response
De la Mare, Jo-Anne (Dr)
Edkins, Adrienne Lesley (Associate Prof)
RU Department of Biochemistry, Microbiology and Biotechnology
SDG 03. Good Health and Well-Being
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