- Title
- Survival of bifidobacteria and their usefulness in faecal source tracking
- Creator
- Luyt, Catherine D
- Creator
- Khamanga, Sandile M
- Creator
- Muller, Wilhelmine J
- Creator
- Tandlich, Roman
- Date Issued
- 2015
- Date
- 2015
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/76117
- Identifier
- vital:30508
- Identifier
- https://doi.org/10.1515/lwr-2015-0001
- Description
- Bifidobacteria have long since been recommended as indicators of human and animal pollution. Concentration ratio (tracking ratio) of the sorbitol-utilising bifidobacteria (SUB) and the total bifidobacteria (TB) can be used to distinguish between animal and human sources of faecal water contamination. The cut-off value needs to be calibrated in a given geographical area. Seven sites with permanent faecal contamination were selected in South Africa. Concentrations of SUB ranged from 10-50000 cells/100 mL, while TB ranged from 0-8000 cells/100 mL. The tracking ratio ranged from 0.10 to 6.25, but no clear cut-off value could be established. The YN-17 agar was replaced for TB with the modified Beerens medium with pH = 5.70, to suppress the growth of faecal streptococci. Tracking ratios observed are most likely the results of different survival rates of SUB and TB. Bifidobacteria die-off due to nutrients was not found to be significant using design of experiment. Thus a lack of continuous input or oxygen levels in water may be major factors. This would limit the ratios used as a faecal source tracking method.
- Format
- Language
- English
- Relation
- Waste Treatment and Recovery
- Relation
- Luyt, C.D., Khamanga, S.M.M., Muller, W.J. and Tandlich, R., 2015. Survival of Bifidobacteria and their usefulness in Faecal Source Tracking. Waste Treatment and Recovery, 1(1).
- Relation
- Waste Treatment and Recovery volume 1 number 1 July 2015 2544-8544
- Rights
- Authors
- Rights
- Use of this resource is governed by the terms and conditions of the De Gruyter Terms and Conditions statement (https://www.degruyter.com/page/218)
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Tandlich, Roman (Dr)
RU Department of Pharmacy
Khamanga, Sandile S M (Assoc Prof)
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