Towards development of a malaria diagnostic: Generation, screening and validation of novel aptamers recognising Plasmodium falciparum lactate dehydrogenase
- Authors: Frith, Kelly-Anne
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Oligonucleotides , Lactate dehydrogenase , Biochemical markers , Systematic evolution of ligands through exponential enrichment (SELEX)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142247 , vital:38062
- Description: Malaria, caused by infection with the Plasmodium parasite, is one of the leading causes of death in under-developed countries. Early detection is crucial for the effective treatment of malaria, particularly in cases where infection is due to Plasmodium falciparum. There is, therefore, an enduring need for portable, sensitive, reliable, accurate, durable, self-validating and cost-effective techniques for the rapid detection of malaria. Moreover, there is a demand to distinguish between various infectious species causing malaria. Research in the area of malarial biomarkers has identified a unique, species-specific, epitope of P. falciparum lactate dehydrogenase (PfLDH), enhancing prospects for the development of diagnostics capable of identifying the species causing malarial infection. In recent years, improvements have been made towards the development of rapid diagnostic tests for detecting malarial biomarkers. Owing to their low cost, ease of labeling, and high thermal stability (relative to antibodies), the development and synthesis of aptamers that target the malarial lactate dehydrogenase represents one of the key innovations in the field of rapid diagnostics for malaria. This study explored the generation of aptamers that specifically target P. falciparum. Two sets of aptamers with diagnostically-supportive functions were generated independently, through parallel SELEX of recombinantly-expressed, full-length Plasmodium falciparum lactate dehydrogenase (rPfLDH), and an oligopeptide comprising the P. falciparum-specific epitope on lactate dehydrogenase (LDHp). The latter offers a promising solution for generating aptamers capable of binding with high specificity to P. falciparum. In this work, an rLDH class of aptamers was generated when SELEX was performed using the full-length rPfLDH protein as the target and the LDHp class of aptamers was generated when SELEX was performed using the oligopeptide LDHp as a target. Aptamers were successfully generated through the process of SELEX (systematic evolution of ligands through exponential enrichment) following the study and application of several optimisation steps, particularly during the amplification stage of SELEX. Optimisation steps included the study of improvements in PCR conditions; role of surfactants (Triton-X), modifying the PCR clean-up protocol; and agarose gel excision. Structurally-relevant moieties with particular consensus sequences (GGTAG and GGCG) were found in aptamers both reported here and previously published, confirming their importance in recognition of the target. Novel moieties particular to this work (ATTAT and poly-A stretches) were identified. Clades of consensus sequences were identified in both the rLDH and LDHp groups of aptamers, where sequences in the rLDH clade did not show preferential binding to rPfLDH while those in the LDHp clade (particularly LDHp 3 and 18) were able to recognise and bind only LDHp. Of the 19 sequences returned from the parallel SELEX procedures for rPfLDH (11 sequences) and LDHp (8 sequences), six rPfLDH and all eight LDHp sequences underwent preliminary screening and those with low responses eliminated. Of the eight LDHp-targeting aptamer sequences, five were preliminarily shown to bind to LDHp, whereas only two rPfLDH-targeting sequences were shown to bind to the target (rLDH 4 and 7). To this small selection of rPfLDH oligonucleotide sequences, two more (rLDH 1 and 15) were chosen for further study based on their sequences, secondary and predicted tertiary conformations. Sequences chosen for further study were therefore: rLDH 1, 4, 7 and 15 in the rLDH class, and LDHp 1, 3, 11, 14 and 18 in the LDHp class. Binding properties of the aptamers towards their targets were investigated using enzyme-linked oligonucleotide assays (ELONA), fluorophore-linked oligonucleotide assays (FLONA), electromobility shift assays (EMSA), surface plasmon resonance (SPR), and GelRed dissociation assays, while applications towards aptasensors were explored using electrochemical impedance spectroscopy (EIS) and fluorescent microscopy. Some inconsistencies were seen for specific aptamer to target binding interactions using specific techniques; however, generally, binding to the targets was observed across the techniques assessed. These varied responses demonstrate the need to screen and validate aptamers using a variety of techniques and platforms not necessarily specific for the proposed application. From the aptamer binding screening studies using ELONA, the most promising aptamers generated were identified as LDHp 11, rLDH 4, rLDH 7 and rLDH 15. Aptamer rLDH 4, which was generated against rPfLDH, exhibited preferential and specific binding to the lactate dehydrogenase from P. falciparum, over the recombinantly-expressed lactate dehydrogenase from Plasmodium vivax (rPvLDH), albeit with lowered responses compared to LDHp 11 in ELONA and EMSA studies. However, in kinetic ELONA studies rLDH 4 showed binding to both rPfLDH and rPvLDH. Aptamer rLDH 7 showed high affinity for rPfLDH and rPvLDH in kinetic studies using ELONA. However, screening studies with ELONA indicates that aptamer rLDH 7 may not be suitable for diagnostic tests in serum samples given its non-specific binding to human serum albumin (HSA). Aptamer rLDH 15 exhibited species specificity for rPfLDH in screening studies using ELONA but showed affinity towards rPvLDH (albeit lower relative to its affinity for rPfLDH) in kinetic studies using ELONA. LDHp 11, generated against the PfLDH peptide, showed a clear preference for rPfLDH when compared to rPvLDH and other control proteins, in both sets of ELONA studies conducted, as well as EMSA, thus possessing a strong ability to identify the presence of Plasmodium falciparum owing to its generation against the species-specific epitope. While LDHp 1 demonstrated binding to plasmodial LDH in a flow-through system (SPR), so reiterating ELONA responses, it did not perform well in the remaining methodologies. Aptamers rLDH 1 and 15 and LDHp 3, 14 and 18 exhibited a mixed set of results throughout the target protein screening analyses and were, thus, not considered for selective binding in P. falciparum parasite bodies. In studies aimed at exploring biosensor assemblies utilising the developed aptamers, both rLDH 4 and LDHp 11, along with rLDH 7, LDHp 1 and pL1, demonstrated in situ binding to the native PfLDH in fluorescent microscopy. LDHp 11 exhibited FITC-based fluorescence equivalent to the anti-rPfLDHp IgY antibody in confocal fluorescent microscopy indicating superior binding to the native PfLDH compared to the remaining aptamers. An examination of electrochemical impedance as a platform for a biosensor assembly did not, in these studies, exhibit the required sensitivity using physiologically relevant concentrations of analyte expected for pLDH following infection with Plasmodium spp. Malstat/LDH activity was explored for application in a colorimetric aptasensor. A decrease in both rPfLDH and rPvLDH activity was observed following incubation with the tested aptamers, but rLDH 1, rLDH 7 and LDHp 14 did not exhibit similar decreases in rPvLDH activity. Aptamers rLDH 1, 4 and 7 and LDHp 11 and 14 were, therefore, not selected as candidates for LDH capture in LDH activity-based diagnostic devices for P. falciparum. The decreases in pLDH activity in the presence of aptamers could hold promise as direct or antagonistic malaria therapeutic agents. Preliminary studies on the application of aptamers as malaria therapeutic agents, while of interest, should be viewed with due caution given the challenges of aptamers reaching the intracellular native plasmodial LDH hosted within the red blood cells. In conclusion, this work has shown the ability of the LDHp 11 aptamer, generated in these studies, to selectively bind rPfLDH over rPvLDH, and to bind to the native PfLDH in fluorescent microscopy, indicating that this aptamer holds promise as a biorecognition element in malaria biosensors and other diagnostic devices for the detection, and differentiation, of P. falciparum and P. vivax. The use of a species-specific epitope of P. falciparum as a target in aptamer generation paves the way for similar such studies aimed at generating aptamers with species selectivity for other Plasmodium species.
- Full Text:
- Date Issued: 2020
- Authors: Frith, Kelly-Anne
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Oligonucleotides , Lactate dehydrogenase , Biochemical markers , Systematic evolution of ligands through exponential enrichment (SELEX)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142247 , vital:38062
- Description: Malaria, caused by infection with the Plasmodium parasite, is one of the leading causes of death in under-developed countries. Early detection is crucial for the effective treatment of malaria, particularly in cases where infection is due to Plasmodium falciparum. There is, therefore, an enduring need for portable, sensitive, reliable, accurate, durable, self-validating and cost-effective techniques for the rapid detection of malaria. Moreover, there is a demand to distinguish between various infectious species causing malaria. Research in the area of malarial biomarkers has identified a unique, species-specific, epitope of P. falciparum lactate dehydrogenase (PfLDH), enhancing prospects for the development of diagnostics capable of identifying the species causing malarial infection. In recent years, improvements have been made towards the development of rapid diagnostic tests for detecting malarial biomarkers. Owing to their low cost, ease of labeling, and high thermal stability (relative to antibodies), the development and synthesis of aptamers that target the malarial lactate dehydrogenase represents one of the key innovations in the field of rapid diagnostics for malaria. This study explored the generation of aptamers that specifically target P. falciparum. Two sets of aptamers with diagnostically-supportive functions were generated independently, through parallel SELEX of recombinantly-expressed, full-length Plasmodium falciparum lactate dehydrogenase (rPfLDH), and an oligopeptide comprising the P. falciparum-specific epitope on lactate dehydrogenase (LDHp). The latter offers a promising solution for generating aptamers capable of binding with high specificity to P. falciparum. In this work, an rLDH class of aptamers was generated when SELEX was performed using the full-length rPfLDH protein as the target and the LDHp class of aptamers was generated when SELEX was performed using the oligopeptide LDHp as a target. Aptamers were successfully generated through the process of SELEX (systematic evolution of ligands through exponential enrichment) following the study and application of several optimisation steps, particularly during the amplification stage of SELEX. Optimisation steps included the study of improvements in PCR conditions; role of surfactants (Triton-X), modifying the PCR clean-up protocol; and agarose gel excision. Structurally-relevant moieties with particular consensus sequences (GGTAG and GGCG) were found in aptamers both reported here and previously published, confirming their importance in recognition of the target. Novel moieties particular to this work (ATTAT and poly-A stretches) were identified. Clades of consensus sequences were identified in both the rLDH and LDHp groups of aptamers, where sequences in the rLDH clade did not show preferential binding to rPfLDH while those in the LDHp clade (particularly LDHp 3 and 18) were able to recognise and bind only LDHp. Of the 19 sequences returned from the parallel SELEX procedures for rPfLDH (11 sequences) and LDHp (8 sequences), six rPfLDH and all eight LDHp sequences underwent preliminary screening and those with low responses eliminated. Of the eight LDHp-targeting aptamer sequences, five were preliminarily shown to bind to LDHp, whereas only two rPfLDH-targeting sequences were shown to bind to the target (rLDH 4 and 7). To this small selection of rPfLDH oligonucleotide sequences, two more (rLDH 1 and 15) were chosen for further study based on their sequences, secondary and predicted tertiary conformations. Sequences chosen for further study were therefore: rLDH 1, 4, 7 and 15 in the rLDH class, and LDHp 1, 3, 11, 14 and 18 in the LDHp class. Binding properties of the aptamers towards their targets were investigated using enzyme-linked oligonucleotide assays (ELONA), fluorophore-linked oligonucleotide assays (FLONA), electromobility shift assays (EMSA), surface plasmon resonance (SPR), and GelRed dissociation assays, while applications towards aptasensors were explored using electrochemical impedance spectroscopy (EIS) and fluorescent microscopy. Some inconsistencies were seen for specific aptamer to target binding interactions using specific techniques; however, generally, binding to the targets was observed across the techniques assessed. These varied responses demonstrate the need to screen and validate aptamers using a variety of techniques and platforms not necessarily specific for the proposed application. From the aptamer binding screening studies using ELONA, the most promising aptamers generated were identified as LDHp 11, rLDH 4, rLDH 7 and rLDH 15. Aptamer rLDH 4, which was generated against rPfLDH, exhibited preferential and specific binding to the lactate dehydrogenase from P. falciparum, over the recombinantly-expressed lactate dehydrogenase from Plasmodium vivax (rPvLDH), albeit with lowered responses compared to LDHp 11 in ELONA and EMSA studies. However, in kinetic ELONA studies rLDH 4 showed binding to both rPfLDH and rPvLDH. Aptamer rLDH 7 showed high affinity for rPfLDH and rPvLDH in kinetic studies using ELONA. However, screening studies with ELONA indicates that aptamer rLDH 7 may not be suitable for diagnostic tests in serum samples given its non-specific binding to human serum albumin (HSA). Aptamer rLDH 15 exhibited species specificity for rPfLDH in screening studies using ELONA but showed affinity towards rPvLDH (albeit lower relative to its affinity for rPfLDH) in kinetic studies using ELONA. LDHp 11, generated against the PfLDH peptide, showed a clear preference for rPfLDH when compared to rPvLDH and other control proteins, in both sets of ELONA studies conducted, as well as EMSA, thus possessing a strong ability to identify the presence of Plasmodium falciparum owing to its generation against the species-specific epitope. While LDHp 1 demonstrated binding to plasmodial LDH in a flow-through system (SPR), so reiterating ELONA responses, it did not perform well in the remaining methodologies. Aptamers rLDH 1 and 15 and LDHp 3, 14 and 18 exhibited a mixed set of results throughout the target protein screening analyses and were, thus, not considered for selective binding in P. falciparum parasite bodies. In studies aimed at exploring biosensor assemblies utilising the developed aptamers, both rLDH 4 and LDHp 11, along with rLDH 7, LDHp 1 and pL1, demonstrated in situ binding to the native PfLDH in fluorescent microscopy. LDHp 11 exhibited FITC-based fluorescence equivalent to the anti-rPfLDHp IgY antibody in confocal fluorescent microscopy indicating superior binding to the native PfLDH compared to the remaining aptamers. An examination of electrochemical impedance as a platform for a biosensor assembly did not, in these studies, exhibit the required sensitivity using physiologically relevant concentrations of analyte expected for pLDH following infection with Plasmodium spp. Malstat/LDH activity was explored for application in a colorimetric aptasensor. A decrease in both rPfLDH and rPvLDH activity was observed following incubation with the tested aptamers, but rLDH 1, rLDH 7 and LDHp 14 did not exhibit similar decreases in rPvLDH activity. Aptamers rLDH 1, 4 and 7 and LDHp 11 and 14 were, therefore, not selected as candidates for LDH capture in LDH activity-based diagnostic devices for P. falciparum. The decreases in pLDH activity in the presence of aptamers could hold promise as direct or antagonistic malaria therapeutic agents. Preliminary studies on the application of aptamers as malaria therapeutic agents, while of interest, should be viewed with due caution given the challenges of aptamers reaching the intracellular native plasmodial LDH hosted within the red blood cells. In conclusion, this work has shown the ability of the LDHp 11 aptamer, generated in these studies, to selectively bind rPfLDH over rPvLDH, and to bind to the native PfLDH in fluorescent microscopy, indicating that this aptamer holds promise as a biorecognition element in malaria biosensors and other diagnostic devices for the detection, and differentiation, of P. falciparum and P. vivax. The use of a species-specific epitope of P. falciparum as a target in aptamer generation paves the way for similar such studies aimed at generating aptamers with species selectivity for other Plasmodium species.
- Full Text:
- Date Issued: 2020
Hop as an anti-cancer drug target
- Vaaltyn, Michaelone Chantelle
- Authors: Vaaltyn, Michaelone Chantelle
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164704 , vital:41156 , doi:10.21504/10962/164704
- Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
- Authors: Vaaltyn, Michaelone Chantelle
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/164704 , vital:41156 , doi:10.21504/10962/164704
- Description: Thesis (PhD)--Rhodes University, Biochemistry and Microbiology, 2020
- Full Text:
- Date Issued: 2020
A management model for human settlements: a case study of Nigeria and South Africa
- Authors: Adeniran, Adeleye Ayoade
- Date: 2020
- Subjects: Human settlements -- Nigeria , Human settlements -- South Africa Sustainable development
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/45985 , vital:39403
- Description: Persistent depreciating human settlements is a global challenge, particularly in developing countries and this challenge contributes to the continuously increasing housing gap, despite several novel policies and programmes. Although there is a dearth of research-based literature on human settlements management, studies show that property management, Facility Management, housing management and urban management impact the sustainability of all these built environment milieus which form part of the human settlements’ sub-components. This research aimed at altering the afore-said challenge by exploring the management of human settlements in Nigeria and in South Africa. Philosophically, the research is pragmatic, and it rests on positivist deductive reasoning. The methods used for data collection include a review of literature, collection of archival records, and empirical studies of purposively selected professionals and stakeholders involved in human settlements’ management in Nigeria and in South Africa. The findings indicate that existing human settlements in the case studies are “good” and “acceptable” with the majority adopting an outsourced method of management. Furthermore, the lack of maintenance records, the deficiency in policy monitoring and the training of management and maintenance personnel were the three highest ranking factors indicated to be affecting human settlements management. Moreover, an inhabitant’s income, the time available for management, professional expertise and technology employed for management are recognised as the critical sustainability sub-factors affecting human settlement management. The factor analysis established ownership, human resource and capital, social, legal, technology, economic, physical and environmental factors as vital and very significant in achieving sustainable management of human settlements. The study recommends the professionalisation of the field of human settlements as well as a formulation of management policy among others. The study developed a management model for human settlements, and it advocates that human settlements managers, owners and organisations should apply the model in this study, to improve their human settlement management practices, so as to achieve sustainability, and in essence, Sustainable Development Goal 11. It is hoped that the suggested management model will influence human settlement policy development and review, the designing of human settlement management programmes including the framework for their ongoing monitoring and evaluation. The study furthermore contributes to the development of housing and human settlement curricula and evolving human settlement research in both developed and developing countries.
- Full Text:
- Date Issued: 2020
- Authors: Adeniran, Adeleye Ayoade
- Date: 2020
- Subjects: Human settlements -- Nigeria , Human settlements -- South Africa Sustainable development
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/45985 , vital:39403
- Description: Persistent depreciating human settlements is a global challenge, particularly in developing countries and this challenge contributes to the continuously increasing housing gap, despite several novel policies and programmes. Although there is a dearth of research-based literature on human settlements management, studies show that property management, Facility Management, housing management and urban management impact the sustainability of all these built environment milieus which form part of the human settlements’ sub-components. This research aimed at altering the afore-said challenge by exploring the management of human settlements in Nigeria and in South Africa. Philosophically, the research is pragmatic, and it rests on positivist deductive reasoning. The methods used for data collection include a review of literature, collection of archival records, and empirical studies of purposively selected professionals and stakeholders involved in human settlements’ management in Nigeria and in South Africa. The findings indicate that existing human settlements in the case studies are “good” and “acceptable” with the majority adopting an outsourced method of management. Furthermore, the lack of maintenance records, the deficiency in policy monitoring and the training of management and maintenance personnel were the three highest ranking factors indicated to be affecting human settlements management. Moreover, an inhabitant’s income, the time available for management, professional expertise and technology employed for management are recognised as the critical sustainability sub-factors affecting human settlement management. The factor analysis established ownership, human resource and capital, social, legal, technology, economic, physical and environmental factors as vital and very significant in achieving sustainable management of human settlements. The study recommends the professionalisation of the field of human settlements as well as a formulation of management policy among others. The study developed a management model for human settlements, and it advocates that human settlements managers, owners and organisations should apply the model in this study, to improve their human settlement management practices, so as to achieve sustainability, and in essence, Sustainable Development Goal 11. It is hoped that the suggested management model will influence human settlement policy development and review, the designing of human settlement management programmes including the framework for their ongoing monitoring and evaluation. The study furthermore contributes to the development of housing and human settlement curricula and evolving human settlement research in both developed and developing countries.
- Full Text:
- Date Issued: 2020
A Strategy for good IT Governance in South African Municipalities
- Authors: Sibanda, Mbusi
- Date: 2020
- Subjects: Information technology -- Management -- South Africa
- Language: English
- Type: Thesis , Doctoral , DIT
- Identifier: http://hdl.handle.net/10948/49573 , vital:41737
- Description: Information technology (IT) is widely used in South African municipalities as a tool for enabling business processes and therefore its design and implementation are critical to municipalities delivering on their core mandate of delivering services to their communities. The implementation of IT relies on the adoption of sound IT governance practices, whose outcome is to ensure that municipalities derive value from the use of IT and minimise the risk associated with it. However, the implementation of IT governance has proved to be challenging in most municipalities. This research sought to devise a strategic approach to the effective and viable implementation of IT governance in municipalities in South Africa. The research was carried out by adopting a design science research approach, which is increasingly being used for studies in the IT discipline as it assists in solving problems through the design of innovative artefacts, in this case a strategy for the implementation of IT governance. To address the objectives, the research was carried out through case studies of South African municipalities which enabled a detailed investigation of phenomena in its natural context. The data was collected by means of mixed methods consisting of a literature review, a survey and semi-structured interviews. A literature review connected to the research problem was done in order to identify the present state of the issues and how they have been confronted. A survey was sent out to municipalities, institutions and individuals with a mandate to support municipalities, while semi-structured interviews were conducted in five municipalities, government departments and regulatory bodies. A thematic analysis of the data collected was performed which led to a diagnosis of the issues. These include inadequate IT infrastructure; lack of coordinated external support; inadequate IT skills; lack of strong and committed IT leadership; poor IT controls; and fragmented planning. Guiding policies and a set of coherent actions for solving the issues were developed. This culminated in the devising of a strategy for the coordinated implementation of IT governance in municipalities. The primary contribution of this study is the formulation of a strategy for the sound implementation of IT governance in South African municipalities. The research contends that by identifying the strategic issues facing municipalities and implementing the proposed actions, most of which fall outside the municipalities, a new contribution to knowledge has been made. Accordingly, the strategy facilitates the development of plans that address the root causes of the poor implementation of IT in municipalities.
- Full Text:
- Date Issued: 2020
- Authors: Sibanda, Mbusi
- Date: 2020
- Subjects: Information technology -- Management -- South Africa
- Language: English
- Type: Thesis , Doctoral , DIT
- Identifier: http://hdl.handle.net/10948/49573 , vital:41737
- Description: Information technology (IT) is widely used in South African municipalities as a tool for enabling business processes and therefore its design and implementation are critical to municipalities delivering on their core mandate of delivering services to their communities. The implementation of IT relies on the adoption of sound IT governance practices, whose outcome is to ensure that municipalities derive value from the use of IT and minimise the risk associated with it. However, the implementation of IT governance has proved to be challenging in most municipalities. This research sought to devise a strategic approach to the effective and viable implementation of IT governance in municipalities in South Africa. The research was carried out by adopting a design science research approach, which is increasingly being used for studies in the IT discipline as it assists in solving problems through the design of innovative artefacts, in this case a strategy for the implementation of IT governance. To address the objectives, the research was carried out through case studies of South African municipalities which enabled a detailed investigation of phenomena in its natural context. The data was collected by means of mixed methods consisting of a literature review, a survey and semi-structured interviews. A literature review connected to the research problem was done in order to identify the present state of the issues and how they have been confronted. A survey was sent out to municipalities, institutions and individuals with a mandate to support municipalities, while semi-structured interviews were conducted in five municipalities, government departments and regulatory bodies. A thematic analysis of the data collected was performed which led to a diagnosis of the issues. These include inadequate IT infrastructure; lack of coordinated external support; inadequate IT skills; lack of strong and committed IT leadership; poor IT controls; and fragmented planning. Guiding policies and a set of coherent actions for solving the issues were developed. This culminated in the devising of a strategy for the coordinated implementation of IT governance in municipalities. The primary contribution of this study is the formulation of a strategy for the sound implementation of IT governance in South African municipalities. The research contends that by identifying the strategic issues facing municipalities and implementing the proposed actions, most of which fall outside the municipalities, a new contribution to knowledge has been made. Accordingly, the strategy facilitates the development of plans that address the root causes of the poor implementation of IT in municipalities.
- Full Text:
- Date Issued: 2020
Investigating the fitness of sea turtles nesting in South Africa
- Le Gouvello du Timat, Diane Z M
- Authors: Le Gouvello du Timat, Diane Z M
- Date: 2020
- Subjects: Sea turtles -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/48445 , vital:40877
- Description: The South African rookeries of the South Western Indian Ocean (SWIO) loggerhead and leatherback regional management units have been continuously monitored for more than 5 decades, representing one of the longest-running sea turtle beach protection and monitoring programmes in the world. Although such conservation has been successful in protecting adults on land when they come to nest, it might not be enough to ensure population recovery if survival is not ensured across all life stages. Sea turtles are particularly vulnerable to direct threats and changing environmental conditions because they are long-lived, slow maturing, and have low survival rates at early life stages, making them conservation-dependent. These characteristics also render them difficult to conserve as a consequence of their wide geographic distributions, spanning multiple jurisdictions and legislative systems with different conservation targets, and a complex life cycle with changes in habitats and diets at different life stages. Sea turtle populations have been severely depleted as a result of historical over-exploitation and current threats including fisheries bycatch, direct harvest, habitat loss, pollution and pathogens, and climate change. In many cases however, population declines have been reversed by a variety of effective conservation measures such as beach protection programs, and marine protected areas, enabling successful population recovery around the world. Despite protection and apparent increase in abundance, indirect pressures can still affect turtle populations. Therefore, it is fundamental to evaluate the effectiveness of conservation measures (using fitness traits such as female and hatchling sizes) and examine underlying trends. The aim of this thesis was thus to evaluate the fitness of loggerhead and leatherback sea turtles nesting in South Africa. Long-term female size trends over time were evaluated and the populations were split between first-time nesters and experienced nesters. As sea turtle reproductive output (clutch size only) is correlated with female size and evidence is showing that in some populations individual female size is decreasing, the effects of maternal body size on reproductive output and hatchling fitness were investigated for both species. Crawling and swimming speeds were used as proxies of hatchling fitness, because these two traits positively influence their survival. An oceanic model was further used in combination with a particle tracking framework to estimate, for the first time, post-hatchling dispersal of loggerhead and leatherback turtles in the SWIO. I investigated whether the trend in female size for loggerhead turtles nesting in South Africa is a result of their foraging strategy to determine if there is a foraging dichotomy (neritic and oceanic). Despite an apparent increasing population, a significant 3.7 and 3.6 cm decrease in the size of both recruit and remingrant female loggerhead turtles, but a stable mean size of leatherbacks for both age groups was observed. I hypothesise that these contradictory effects are attributable to the very distinct foraging ecologies of the two turtle species and speculate that these variations in carapace length may be driven by environmental change. The reproductive strategy of both loggerhead and leatherback turtles nesting in South Africa supports the optimal egg size theory with larger turtles producing larger clutches but not larger eggs. Egg size was thus constant irrespective of maternal length. Hatchlings with longer carapaces and flippers had higher swimming speed, suggesting that larger hatchlings are fitter (potentially higher survival) than smaller ones by spending less time in high predation risk areas (i.e. beach and nearshore). The model revealed that dispersal trajectories of both loggerhead and leatherback sea turtle hatchlings were very similar and simulations indicated that initial active swimming (frenzy) as well as variability in oceanic conditions strongly influenced dispersal of virtual hatchlings. The trends observed in this study emphasize the importance of long-term monitoring, examining not only abundance but also individual size, to understand population dynamics, support recovery planning and prioritize future conservation practices. Furthermore, the findings of this study have important implications for overall population growth if smaller hatchlings of lower fitness are produced as a result of climate change and thus have decreased dispersal abilities and lower likelihood of survival.
- Full Text:
- Date Issued: 2020
- Authors: Le Gouvello du Timat, Diane Z M
- Date: 2020
- Subjects: Sea turtles -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/48445 , vital:40877
- Description: The South African rookeries of the South Western Indian Ocean (SWIO) loggerhead and leatherback regional management units have been continuously monitored for more than 5 decades, representing one of the longest-running sea turtle beach protection and monitoring programmes in the world. Although such conservation has been successful in protecting adults on land when they come to nest, it might not be enough to ensure population recovery if survival is not ensured across all life stages. Sea turtles are particularly vulnerable to direct threats and changing environmental conditions because they are long-lived, slow maturing, and have low survival rates at early life stages, making them conservation-dependent. These characteristics also render them difficult to conserve as a consequence of their wide geographic distributions, spanning multiple jurisdictions and legislative systems with different conservation targets, and a complex life cycle with changes in habitats and diets at different life stages. Sea turtle populations have been severely depleted as a result of historical over-exploitation and current threats including fisheries bycatch, direct harvest, habitat loss, pollution and pathogens, and climate change. In many cases however, population declines have been reversed by a variety of effective conservation measures such as beach protection programs, and marine protected areas, enabling successful population recovery around the world. Despite protection and apparent increase in abundance, indirect pressures can still affect turtle populations. Therefore, it is fundamental to evaluate the effectiveness of conservation measures (using fitness traits such as female and hatchling sizes) and examine underlying trends. The aim of this thesis was thus to evaluate the fitness of loggerhead and leatherback sea turtles nesting in South Africa. Long-term female size trends over time were evaluated and the populations were split between first-time nesters and experienced nesters. As sea turtle reproductive output (clutch size only) is correlated with female size and evidence is showing that in some populations individual female size is decreasing, the effects of maternal body size on reproductive output and hatchling fitness were investigated for both species. Crawling and swimming speeds were used as proxies of hatchling fitness, because these two traits positively influence their survival. An oceanic model was further used in combination with a particle tracking framework to estimate, for the first time, post-hatchling dispersal of loggerhead and leatherback turtles in the SWIO. I investigated whether the trend in female size for loggerhead turtles nesting in South Africa is a result of their foraging strategy to determine if there is a foraging dichotomy (neritic and oceanic). Despite an apparent increasing population, a significant 3.7 and 3.6 cm decrease in the size of both recruit and remingrant female loggerhead turtles, but a stable mean size of leatherbacks for both age groups was observed. I hypothesise that these contradictory effects are attributable to the very distinct foraging ecologies of the two turtle species and speculate that these variations in carapace length may be driven by environmental change. The reproductive strategy of both loggerhead and leatherback turtles nesting in South Africa supports the optimal egg size theory with larger turtles producing larger clutches but not larger eggs. Egg size was thus constant irrespective of maternal length. Hatchlings with longer carapaces and flippers had higher swimming speed, suggesting that larger hatchlings are fitter (potentially higher survival) than smaller ones by spending less time in high predation risk areas (i.e. beach and nearshore). The model revealed that dispersal trajectories of both loggerhead and leatherback sea turtle hatchlings were very similar and simulations indicated that initial active swimming (frenzy) as well as variability in oceanic conditions strongly influenced dispersal of virtual hatchlings. The trends observed in this study emphasize the importance of long-term monitoring, examining not only abundance but also individual size, to understand population dynamics, support recovery planning and prioritize future conservation practices. Furthermore, the findings of this study have important implications for overall population growth if smaller hatchlings of lower fitness are produced as a result of climate change and thus have decreased dispersal abilities and lower likelihood of survival.
- Full Text:
- Date Issued: 2020
Water balance processes in indigenous and introduced spaces three production system in the Southern Cape region of South Africa
- Authors: Mapeto, Tatenda
- Date: 2020
- Subjects: Hydrology -- Research -- South Africa , Groundwater -- Research -- South Africa Trees -- Breeding Sustainable forestry
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49325 , vital:41621
- Description: Forests and trees are important modulators of water flow and their position in the landscape impacts the spatial and temporal availability of ground water and surface water resources. As such, the global extent of natural forests is linked to the availability of water. In the circumstances where tree production systems have been introduced and are managed in landscapes previously populated with other forms of vegetation, it is necessary to understand their hydrological impact so as to ensure that the benefits derived from these tree systems do not outweigh the costs of water availability for sustaining human and environmental water requirements.
- Full Text: false
- Date Issued: 2020
- Authors: Mapeto, Tatenda
- Date: 2020
- Subjects: Hydrology -- Research -- South Africa , Groundwater -- Research -- South Africa Trees -- Breeding Sustainable forestry
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49325 , vital:41621
- Description: Forests and trees are important modulators of water flow and their position in the landscape impacts the spatial and temporal availability of ground water and surface water resources. As such, the global extent of natural forests is linked to the availability of water. In the circumstances where tree production systems have been introduced and are managed in landscapes previously populated with other forms of vegetation, it is necessary to understand their hydrological impact so as to ensure that the benefits derived from these tree systems do not outweigh the costs of water availability for sustaining human and environmental water requirements.
- Full Text: false
- Date Issued: 2020
The role of custodial grandparents in psychosocial well-being of adolescents from single parents in Buffalo City Municipality of Eastern Cape, South Africa
- Authors: Adenike,Folorunsho Fausat
- Date: 2020
- Subjects: Grandparents as parents Grandparent and child Grandparenting
- Language: English
- Type: Thesis , Doctoral , PhD(Social Work)
- Identifier: http://hdl.handle.net/10353/17943 , vital:41994
- Description: It has been a historical norm in African societies for children to leave in the homes of caregivers. Among these caregivers are the grandparents, who are from time immemorial, being a pillar of support to their children in caring for their grandchildren. Against this background, the study assesses custodial the role of grandparents in the psychosocial well-being of adolescent children from single parents' homes in Buffalo City Metropolitan Municipality, Eastern Cape Province, South Africa. The study was guided theoretically by Role and Ryff's psychosocial well-being theories. Four sets of participants totaling 323 gathered using a mixed-method approach comprised of 150 adolescents and 136 custodial grandparents through questionnaires, 30 single parents, through in-depth interviews, and seven social workers through focus group discussions. The study utilized a multi-stage sampling technique to select adolescents and their grandparents, a purposive sampling technique for single parents, and a convenient sampling technique for social workers. The quantitative data were analyzed using descriptive statistics and Pearson's Correlation Coefficient, while the qualitative data were categorized manually into themes and subthemes. Findings indicated a correlation between the attainment of the adolescent children's psychosocial well-being and the effectiveness of the custodial grandparents' role. More also, the challenges encountered by custodial grandparents do affect the attainment of the psychosocial well-being of adolescent children. Furthermore, finding from the study revealed that non-governmental organizations in the municipality were not rendering social support for the custodial grandparents. Finally, the child-support grant received by poor grandparents is grossly inadequate to meet up with the astronomical needs of the adolescent children. The study concluded that custodial grandparents' social support is an effective mechanism in meeting adolescent children's psychosocial well-being. Several recommendations were made from the conclusions to various stakeholders and proposed a responsive conceptual grand-parenting model to cater for effective parenting.
- Full Text:
- Date Issued: 2020
- Authors: Adenike,Folorunsho Fausat
- Date: 2020
- Subjects: Grandparents as parents Grandparent and child Grandparenting
- Language: English
- Type: Thesis , Doctoral , PhD(Social Work)
- Identifier: http://hdl.handle.net/10353/17943 , vital:41994
- Description: It has been a historical norm in African societies for children to leave in the homes of caregivers. Among these caregivers are the grandparents, who are from time immemorial, being a pillar of support to their children in caring for their grandchildren. Against this background, the study assesses custodial the role of grandparents in the psychosocial well-being of adolescent children from single parents' homes in Buffalo City Metropolitan Municipality, Eastern Cape Province, South Africa. The study was guided theoretically by Role and Ryff's psychosocial well-being theories. Four sets of participants totaling 323 gathered using a mixed-method approach comprised of 150 adolescents and 136 custodial grandparents through questionnaires, 30 single parents, through in-depth interviews, and seven social workers through focus group discussions. The study utilized a multi-stage sampling technique to select adolescents and their grandparents, a purposive sampling technique for single parents, and a convenient sampling technique for social workers. The quantitative data were analyzed using descriptive statistics and Pearson's Correlation Coefficient, while the qualitative data were categorized manually into themes and subthemes. Findings indicated a correlation between the attainment of the adolescent children's psychosocial well-being and the effectiveness of the custodial grandparents' role. More also, the challenges encountered by custodial grandparents do affect the attainment of the psychosocial well-being of adolescent children. Furthermore, finding from the study revealed that non-governmental organizations in the municipality were not rendering social support for the custodial grandparents. Finally, the child-support grant received by poor grandparents is grossly inadequate to meet up with the astronomical needs of the adolescent children. The study concluded that custodial grandparents' social support is an effective mechanism in meeting adolescent children's psychosocial well-being. Several recommendations were made from the conclusions to various stakeholders and proposed a responsive conceptual grand-parenting model to cater for effective parenting.
- Full Text:
- Date Issued: 2020
The role of microhabitats within mangroves: an invertebrate and fish larval perspective
- Authors: Vorsatz, Lyle Dennis
- Date: 2020
- Subjects: Mangrove ecology -- South Africa , Mangrove forests -- South Africa , Niche (Ecology) , Rhizophora mucronata , Acanthaceae , Rhizophoraceae , Fishes -- Larvae -- South Africa , Aquatic ecology -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167644 , vital:41499
- Description: Microhabitats provided through structural complexity are central for the diversity, productivity, connectivity and niche differentiation within and among ecosystems. Mangrove forests afford juvenile fish and invertebrates with nursery and recruitment habitats, facilitated by the fine scale configuration of their specialised root systems. Although the importance of mangroves for resident and transient juveniles is well recognised, the roles that mangrove microhabitats play for larvae is not yet comprehensively understood. This study aimed to determine how microhabitats with varying degrees of complexity influence the composition, abundance and distribution of larval communities that inhabit mangrove forests and the physiological responses of larvae to acute temperature variations in relation to ontogenetic stage and microenvironment exposure. Two relatively pristine study sites were selected to represent a warm temperate and subtropical mangrove system in the Eastern Cape and KwaZulu-Natal on the east coast of South Africa, respectively. The differences in complexity among the root systems of Rhizophora mucronata, Avicennia marina and Bruguiera gymnorhiza were assessed using 3D scanning and the computed 3D models were then analysed using four complexity metrics. Results indicated that A. marina is the most complex in terms of surface-volume ratio, R. mucronata has the most interstitial space among its roots and B. gymnorhiza and R. mucronata differ in their fractal dimensions. Larvae collected in each microhabitat at each site using light traps showed that, despite temperature and salinity homogeneity across microenvironments, spatio-temporal differences occurred in both fish and invertebrate assemblages. This trend suggests that microhabitat structural complexity exerts an influence on larval community composition by acting as a microscape of available habitat, which ensures ecological linkages within and among the mangrove forest and adjacent ecosystems. In addition, the oxygen consumption rates of mangrove-associated brachyuran larvae varied according to mangrove microhabitat, whereby larvae collected at less complex environments had the highest metabolic rates at increased temperatures. Moreover, ontogenetic shifts in physiology were prevalent as older brachyuran larvae were more eurythermal than earlier stages, suggesting that thermally stressful events will have a greater impact on recently spawned larvae. Overall, the interstitial spaces within individual root systems are the most important complexity measure, as utilisation of these mangrove microhabitats is scale-dependent, and larvae will most likely occupy spaces inaccessible to large predators. Likewise, microscale variation in the environmental conditions and ontogenetic stage of brachyuran larvae within the mangrove microscape, can amplify the physiological responses to rapid temperature variations. Results suggest that early stage larvae are the most vulnerable to mass-mortality, and if thermally stressful events increase in frequency, duration and magnitude, the larval supply for the successful recruitment into adult populations could be under threat. Through linking how mangrove microhabitat complexity influences larvae in terms of community metrics and physiology, this study paves the way for further advancement of our understanding of how microscale processes emerge into meso- and macroscale patterns and influence the stability and functioning of highly productive ecosystems.
- Full Text:
- Date Issued: 2020
- Authors: Vorsatz, Lyle Dennis
- Date: 2020
- Subjects: Mangrove ecology -- South Africa , Mangrove forests -- South Africa , Niche (Ecology) , Rhizophora mucronata , Acanthaceae , Rhizophoraceae , Fishes -- Larvae -- South Africa , Aquatic ecology -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167644 , vital:41499
- Description: Microhabitats provided through structural complexity are central for the diversity, productivity, connectivity and niche differentiation within and among ecosystems. Mangrove forests afford juvenile fish and invertebrates with nursery and recruitment habitats, facilitated by the fine scale configuration of their specialised root systems. Although the importance of mangroves for resident and transient juveniles is well recognised, the roles that mangrove microhabitats play for larvae is not yet comprehensively understood. This study aimed to determine how microhabitats with varying degrees of complexity influence the composition, abundance and distribution of larval communities that inhabit mangrove forests and the physiological responses of larvae to acute temperature variations in relation to ontogenetic stage and microenvironment exposure. Two relatively pristine study sites were selected to represent a warm temperate and subtropical mangrove system in the Eastern Cape and KwaZulu-Natal on the east coast of South Africa, respectively. The differences in complexity among the root systems of Rhizophora mucronata, Avicennia marina and Bruguiera gymnorhiza were assessed using 3D scanning and the computed 3D models were then analysed using four complexity metrics. Results indicated that A. marina is the most complex in terms of surface-volume ratio, R. mucronata has the most interstitial space among its roots and B. gymnorhiza and R. mucronata differ in their fractal dimensions. Larvae collected in each microhabitat at each site using light traps showed that, despite temperature and salinity homogeneity across microenvironments, spatio-temporal differences occurred in both fish and invertebrate assemblages. This trend suggests that microhabitat structural complexity exerts an influence on larval community composition by acting as a microscape of available habitat, which ensures ecological linkages within and among the mangrove forest and adjacent ecosystems. In addition, the oxygen consumption rates of mangrove-associated brachyuran larvae varied according to mangrove microhabitat, whereby larvae collected at less complex environments had the highest metabolic rates at increased temperatures. Moreover, ontogenetic shifts in physiology were prevalent as older brachyuran larvae were more eurythermal than earlier stages, suggesting that thermally stressful events will have a greater impact on recently spawned larvae. Overall, the interstitial spaces within individual root systems are the most important complexity measure, as utilisation of these mangrove microhabitats is scale-dependent, and larvae will most likely occupy spaces inaccessible to large predators. Likewise, microscale variation in the environmental conditions and ontogenetic stage of brachyuran larvae within the mangrove microscape, can amplify the physiological responses to rapid temperature variations. Results suggest that early stage larvae are the most vulnerable to mass-mortality, and if thermally stressful events increase in frequency, duration and magnitude, the larval supply for the successful recruitment into adult populations could be under threat. Through linking how mangrove microhabitat complexity influences larvae in terms of community metrics and physiology, this study paves the way for further advancement of our understanding of how microscale processes emerge into meso- and macroscale patterns and influence the stability and functioning of highly productive ecosystems.
- Full Text:
- Date Issued: 2020
Ecological assessment of a temperate river system using biomonitoring techniques: a case study of the Bloukrans River system, South Africa
- Authors: Magadze, Tinotenda
- Date: 2020
- Subjects: Bloukrans River , Ecological assessment (Biology) -- South Africa , Stream ecology -- South Africa , Rivers -- Environmental aspects -- South Africa , Benthic animals -- Effect of human beings on -- South Africa , Water quality biological assessment -- South Africa , Diatoms -- South Africa -- Data processing
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142933 , vital:38177
- Description: Escalating pressures from growing human populations and environmental impacts increasingly imperil freshwater ecosystems. The Bloukrans River, which drains an urbanised and agricultural catchment in the Eastern Cape province of South Africa, is no exception. Detailed knowledge of the structure and function of the aquatic ecosystems is required in order to create models and matrices that predict, guide assessment and direct intervention on ecological integrity and water quality management in these systems. The main objectives of this research were to: examine the effects of urbanization on the benthic macroinvertebrate functional feeding guild structure among different stream orders; determine if benthic diatoms can be used as effective and reliable indicators of ionic composition and conductivity in different stream order categories and finally; to evaluate the applicability of the South African Diatom Index (SADI) and other indices in the Eastern Cape region of South Africa. Field studies were carried out to explore temporal patterns in community structure (macroinvertebrates and diatoms) and ecosystem function related to land-use patterns, instream habitat availability, and water quality parameters in the Bloukrans River system across four study periods: February (summer) and July (winter) 2016 and February (summer) and May/July (winter) 2018. The study was conducted along a gradient of impacts from less impacted agricultural headwaters to highly impacted urban sites located immediately downstream of the city of Makhanda. Macroinvertebrates were separated into functional feeding groups (FFGs) (i.e. collector-gatherer, collector-filterer, scraper, shredder, and predator) which were then used to assess the effects of selected physico-chemical variables and riparian zone condition on FFG organization. Collector-gatherers were the most abundant in the Bloukrans River and represented 71.3 % of the macroinvertebrate assemblages. Stream order 1species such as Nitzschia palea, Gomphonema parvulum, Tryblionella apiculata, Diploneis vulgaris and Staurosira elliptica. Multivariate analysis (Canonical correspondence analysis (CCA)) indicated that differences in diatom community assemblages were best explained by calcium, magnesium, pH, phosphate, nitrate, dissolved oxygen, sediment nitrate, conductivity and salinity. These results indicate that diatoms can be used as bioindicators for monitoring highly impacted river systems and to also further examine pollution gradients and impacts of specific/point pollution sources. In order to further test the application of diatom indices, nine sites with contrasting water quality were sampled along the length river system in February, May and July 2018. Diatom-based indices incorporated in OMNIDIA software were applied to assess the integrity of the water quality as indicated by diatom communities. For comparative purposes, several foreign indices (e.g. the trophic diatom index (TDI), the percentage pollution-tolerant valves (%PTV), biological diatom index (BDI)) and the South African Diatom Index (SADI) were used in the study. From the results, the Percentage Pollution-Tolerant Valves (%PTV) of most urban sites in the Bloukrans River was above the 20% limit indicating the presence of organic pollutants. Although the foreign diatom indices were applicable in the study, the SADI had significant correlations with most water quality variables (p < 0.05) compared to other indices such as Watanabe Index (WAT), Biological Index of Water Quality Trophic Index (BIWQ) and Trophic Index (TI)). These results support wider use of the SADI as an indicator of water quality conditions in South African river systems. Finally, the observed variations in diatom community structure and composition as a result of changes in water quality were broadly in agreement with the results of macroinvertebrate FFG structure indicating that the two biological indicators can, and should, be used as complementary techniques in the biomonitoring of rivers and streams in South Africa.
- Full Text:
- Date Issued: 2020
- Authors: Magadze, Tinotenda
- Date: 2020
- Subjects: Bloukrans River , Ecological assessment (Biology) -- South Africa , Stream ecology -- South Africa , Rivers -- Environmental aspects -- South Africa , Benthic animals -- Effect of human beings on -- South Africa , Water quality biological assessment -- South Africa , Diatoms -- South Africa -- Data processing
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142933 , vital:38177
- Description: Escalating pressures from growing human populations and environmental impacts increasingly imperil freshwater ecosystems. The Bloukrans River, which drains an urbanised and agricultural catchment in the Eastern Cape province of South Africa, is no exception. Detailed knowledge of the structure and function of the aquatic ecosystems is required in order to create models and matrices that predict, guide assessment and direct intervention on ecological integrity and water quality management in these systems. The main objectives of this research were to: examine the effects of urbanization on the benthic macroinvertebrate functional feeding guild structure among different stream orders; determine if benthic diatoms can be used as effective and reliable indicators of ionic composition and conductivity in different stream order categories and finally; to evaluate the applicability of the South African Diatom Index (SADI) and other indices in the Eastern Cape region of South Africa. Field studies were carried out to explore temporal patterns in community structure (macroinvertebrates and diatoms) and ecosystem function related to land-use patterns, instream habitat availability, and water quality parameters in the Bloukrans River system across four study periods: February (summer) and July (winter) 2016 and February (summer) and May/July (winter) 2018. The study was conducted along a gradient of impacts from less impacted agricultural headwaters to highly impacted urban sites located immediately downstream of the city of Makhanda. Macroinvertebrates were separated into functional feeding groups (FFGs) (i.e. collector-gatherer, collector-filterer, scraper, shredder, and predator) which were then used to assess the effects of selected physico-chemical variables and riparian zone condition on FFG organization. Collector-gatherers were the most abundant in the Bloukrans River and represented 71.3 % of the macroinvertebrate assemblages. Stream order 1species such as Nitzschia palea, Gomphonema parvulum, Tryblionella apiculata, Diploneis vulgaris and Staurosira elliptica. Multivariate analysis (Canonical correspondence analysis (CCA)) indicated that differences in diatom community assemblages were best explained by calcium, magnesium, pH, phosphate, nitrate, dissolved oxygen, sediment nitrate, conductivity and salinity. These results indicate that diatoms can be used as bioindicators for monitoring highly impacted river systems and to also further examine pollution gradients and impacts of specific/point pollution sources. In order to further test the application of diatom indices, nine sites with contrasting water quality were sampled along the length river system in February, May and July 2018. Diatom-based indices incorporated in OMNIDIA software were applied to assess the integrity of the water quality as indicated by diatom communities. For comparative purposes, several foreign indices (e.g. the trophic diatom index (TDI), the percentage pollution-tolerant valves (%PTV), biological diatom index (BDI)) and the South African Diatom Index (SADI) were used in the study. From the results, the Percentage Pollution-Tolerant Valves (%PTV) of most urban sites in the Bloukrans River was above the 20% limit indicating the presence of organic pollutants. Although the foreign diatom indices were applicable in the study, the SADI had significant correlations with most water quality variables (p < 0.05) compared to other indices such as Watanabe Index (WAT), Biological Index of Water Quality Trophic Index (BIWQ) and Trophic Index (TI)). These results support wider use of the SADI as an indicator of water quality conditions in South African river systems. Finally, the observed variations in diatom community structure and composition as a result of changes in water quality were broadly in agreement with the results of macroinvertebrate FFG structure indicating that the two biological indicators can, and should, be used as complementary techniques in the biomonitoring of rivers and streams in South Africa.
- Full Text:
- Date Issued: 2020
Understanding climate change and rural livelihoods in Zimbabwe: adaptation by communal farmers in Ngundu, Chivi District
- Authors: Nciizah, Elinah
- Date: 2020
- Subjects: Agriculture -- Zimbabwe , Agriculture -- Climatic factors -- Chivi District (Zimbabwe) , Chivi District (Zimbabwe) -- Rural conditions
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/118765 , vital:34666
- Description: Climate change and variability is a global phenomenon which has deeply localised patterns, dynamics and effects. Amongst those people who are particularly vulnerable to climate change effects are small-scale farmers who are dependent in large part on rain-fed agriculture in pursuing their livelihoods. This is true of small-scale farmers in contemporary Zimbabwe and, more specifically, farmers in communal areas. At the same time, at international and national levels, there are attempts currently to minimise the effects of, and to adapt to, climate change. However, adaptation measures also exist at local levels amongst small-scale farmers, such as communal farmers in Zimbabwe. In this context, as its main objective, this thesis examines climate change and small-scale farmer livelihood adaptation to climate change with specific reference to communal farmers in Chivi District in Zimbabwe and, in particular, in Ward 25 which is popularly known as Ngundu. In pursuing this main objective, a number of subsidiary objectives are addressed, including a focus on the established livelihoods of Ngundu farmers, the perceptions and concerns of Ngundu farmers about climate change, the coping and adaptation measures of Ngundu farmers, and the enablements and constraints which affect attempts by Ngundu farmers to adopt such measures. The fieldwork for the thesis involved a diverse array of research methods, such as a questionnaire survey, life-history interviews, key informant interviews, focus group discussions and transect walks. In terms of theoretical framing, the thesis makes use of both middle-level theory (the Sustainable Livelihoods Framework) and macro-theory in the form of the sociological work of Margaret Archer. Combined, these two theories allow for a focus on both structure and agency when seeking to understand livelihood adaptations to climate change by communal farmers in Ngundu. The thesis concludes that there are massive constraints inhibiting adaptation measures by Ngundu farmers, but that this should not distract from the deep, often historically-embedded, concerns of Ngundu farmers about climate change and the multiple ways in which they express agency in and through adaptation and coping activities. It also highlights the need for more specifically sociological investigations of climate change and small-scale farmer adaptation, as well as the need for localised studies which are able to identify and analyse the specificities of adaptation.
- Full Text:
- Date Issued: 2020
- Authors: Nciizah, Elinah
- Date: 2020
- Subjects: Agriculture -- Zimbabwe , Agriculture -- Climatic factors -- Chivi District (Zimbabwe) , Chivi District (Zimbabwe) -- Rural conditions
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/118765 , vital:34666
- Description: Climate change and variability is a global phenomenon which has deeply localised patterns, dynamics and effects. Amongst those people who are particularly vulnerable to climate change effects are small-scale farmers who are dependent in large part on rain-fed agriculture in pursuing their livelihoods. This is true of small-scale farmers in contemporary Zimbabwe and, more specifically, farmers in communal areas. At the same time, at international and national levels, there are attempts currently to minimise the effects of, and to adapt to, climate change. However, adaptation measures also exist at local levels amongst small-scale farmers, such as communal farmers in Zimbabwe. In this context, as its main objective, this thesis examines climate change and small-scale farmer livelihood adaptation to climate change with specific reference to communal farmers in Chivi District in Zimbabwe and, in particular, in Ward 25 which is popularly known as Ngundu. In pursuing this main objective, a number of subsidiary objectives are addressed, including a focus on the established livelihoods of Ngundu farmers, the perceptions and concerns of Ngundu farmers about climate change, the coping and adaptation measures of Ngundu farmers, and the enablements and constraints which affect attempts by Ngundu farmers to adopt such measures. The fieldwork for the thesis involved a diverse array of research methods, such as a questionnaire survey, life-history interviews, key informant interviews, focus group discussions and transect walks. In terms of theoretical framing, the thesis makes use of both middle-level theory (the Sustainable Livelihoods Framework) and macro-theory in the form of the sociological work of Margaret Archer. Combined, these two theories allow for a focus on both structure and agency when seeking to understand livelihood adaptations to climate change by communal farmers in Ngundu. The thesis concludes that there are massive constraints inhibiting adaptation measures by Ngundu farmers, but that this should not distract from the deep, often historically-embedded, concerns of Ngundu farmers about climate change and the multiple ways in which they express agency in and through adaptation and coping activities. It also highlights the need for more specifically sociological investigations of climate change and small-scale farmer adaptation, as well as the need for localised studies which are able to identify and analyse the specificities of adaptation.
- Full Text:
- Date Issued: 2020
Understanding biotic interactions in invaded pond communities in the Sundays River irrigation network, South Africa
- Authors: Mofu, Lubabalo
- Date: 2020
- Subjects: Mozambique tilapia -- South Africa -- Sundays River (Eastern Cape) , Western mosquitofish -- South Africa -- Sundays River (Eastern Cape) , Gobiidae -- South Africa -- Sundays River (Eastern Cape) , Clupeidae -- South Africa -- Sundays River (Eastern Cape) , Reservoirs -- South Africa -- Sundays River (Eastern Cape) , Zooplankton -- South Africa -- Sundays River (Eastern Cape) , Freshwater ecology -- South Africa -- Sundays River (Eastern Cape) , Biotic communities -- South Africa -- Sundays River (Eastern Cape) , Fishes -- Effect of temperature on -- South Africa -- Sundays River (Eastern Cape) , Stable isotopes , Relative Impact Potential
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167089 , vital:41436
- Description: The Sundays River valley irrigation ponds provide a unique opportunity to investigate biotic interactions within a biological invasions context, as they contain both native and non-native fish species. This study focusses on two native species (Glossogobius callidus and Gilchristella aestuaria) and two non-native species (Oreochromis mossambicus and Gambusia affinis). The ecology of the ponds was driven by physico-chemical variables, mainly temperature, but the interactions between fishes were a complex interplay between temperature, pond community ecology and food web structure. Seasonal changes in temperature and subsequent fluctuations in water levels resulted in changes in zooplankton community. Chlorophyll-a, temperature, G. callidus and G. affinis were the drivers of the seasonal changes in macroinvertebrate composition. Stable isotope analysis identified substantial ontogenetic dietary shifts in all species, corresponding to changes in body size. Stable isotope analysis revealed that the niche space occupied by G. affinis was broad and overlapped with that of the other three focal species. Stable isotope metrics showed that G. affinis and O. mossambicus utilised a wide range of resources compared to G. callidus and G. aestuaria. Stomach content analysis showed that G. callidus, O. mossambicus and G. affinis fed predominantly on benthic resources, while G. aestuaria fed mainly plankton resources. Functional response experiments revealed that G. callidus and G. affinis both displayed Type II functional responses. In single fish trials, G. affinis had significantly higher functional responses than G. callidus. In heterospecific G. callidus-G. affinis combinations the functional response of G. callidus was reduced by the presence of G. affinis, whereas, this combination greatly enhanced G. affinis functional response magnitudes. The functional response of G. callidus, O. mossambicus and G. affinis under two temperature treatments along with fish abundance data was used to determine temporal differences in the ecological impacts of each fish species between seasons. The relative impact potential of O. mossambicus was consistently higher than that of G. callidus and G. affinis. This study demonstrates how seasonal temperature fluctuations affect the relative impact capacities of introduced species. Overall, this thesis showed that high temperature along with life-history traits contributes to the biotic interactions between native and non-native species in novel environments.
- Full Text:
- Date Issued: 2020
- Authors: Mofu, Lubabalo
- Date: 2020
- Subjects: Mozambique tilapia -- South Africa -- Sundays River (Eastern Cape) , Western mosquitofish -- South Africa -- Sundays River (Eastern Cape) , Gobiidae -- South Africa -- Sundays River (Eastern Cape) , Clupeidae -- South Africa -- Sundays River (Eastern Cape) , Reservoirs -- South Africa -- Sundays River (Eastern Cape) , Zooplankton -- South Africa -- Sundays River (Eastern Cape) , Freshwater ecology -- South Africa -- Sundays River (Eastern Cape) , Biotic communities -- South Africa -- Sundays River (Eastern Cape) , Fishes -- Effect of temperature on -- South Africa -- Sundays River (Eastern Cape) , Stable isotopes , Relative Impact Potential
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167089 , vital:41436
- Description: The Sundays River valley irrigation ponds provide a unique opportunity to investigate biotic interactions within a biological invasions context, as they contain both native and non-native fish species. This study focusses on two native species (Glossogobius callidus and Gilchristella aestuaria) and two non-native species (Oreochromis mossambicus and Gambusia affinis). The ecology of the ponds was driven by physico-chemical variables, mainly temperature, but the interactions between fishes were a complex interplay between temperature, pond community ecology and food web structure. Seasonal changes in temperature and subsequent fluctuations in water levels resulted in changes in zooplankton community. Chlorophyll-a, temperature, G. callidus and G. affinis were the drivers of the seasonal changes in macroinvertebrate composition. Stable isotope analysis identified substantial ontogenetic dietary shifts in all species, corresponding to changes in body size. Stable isotope analysis revealed that the niche space occupied by G. affinis was broad and overlapped with that of the other three focal species. Stable isotope metrics showed that G. affinis and O. mossambicus utilised a wide range of resources compared to G. callidus and G. aestuaria. Stomach content analysis showed that G. callidus, O. mossambicus and G. affinis fed predominantly on benthic resources, while G. aestuaria fed mainly plankton resources. Functional response experiments revealed that G. callidus and G. affinis both displayed Type II functional responses. In single fish trials, G. affinis had significantly higher functional responses than G. callidus. In heterospecific G. callidus-G. affinis combinations the functional response of G. callidus was reduced by the presence of G. affinis, whereas, this combination greatly enhanced G. affinis functional response magnitudes. The functional response of G. callidus, O. mossambicus and G. affinis under two temperature treatments along with fish abundance data was used to determine temporal differences in the ecological impacts of each fish species between seasons. The relative impact potential of O. mossambicus was consistently higher than that of G. callidus and G. affinis. This study demonstrates how seasonal temperature fluctuations affect the relative impact capacities of introduced species. Overall, this thesis showed that high temperature along with life-history traits contributes to the biotic interactions between native and non-native species in novel environments.
- Full Text:
- Date Issued: 2020
A combined modelling approach for simulating channel–wetland exchanges in large African river basins
- Authors: Makungu, Eunice J
- Date: 2020
- Subjects: Watersheds -- Africa , Watershed management -- Africa , Water resources development -- Africa -- International cooperation , Floodplain management -- Africa , Wetland ecology -- Simulation methods -- Africa , Wetland management -- Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/123288 , vital:35424
- Description: In Africa, many large and extensive wetlands are hydrologically connected to rivers, and their environmental integrity, as well as their influence on downstream flow regimes, depends on the prevailing channel–wetland exchange processes. These processes are inherently complex and vary spatially and temporally. Understanding channel–wetland exchanges is therefore, indispensable for the effective management of wetlands and the associated river basins. However, this information is limited in most of the river basins containing large wetlands in Africa. Furthermore, it is important to understand the links between upstream and downstream flow regimes and the wetland dynamics themselves, specifically where there are water resource developments that may affect these links (upstream developments), or be affected by them (downstream developments). Hydrological modelling of the entire basin using basin-scale models that include wetland components in their structures can be used to provide the information required to manage water resources in such basins. However, the level of detail of wetland processes included in many basin-scale models is typically very low and the lack of understanding of the wetland dynamics makes it difficult to quantify the relevant parameters. Detailed hydraulic models represent the channel-wetland exchanges in a much more explicit manner, but require relatively more data and time resources to establish than coarser scale hydrological models. The main objective of this study was, therefore, to investigate the use of a detailed hydraulic wetland model to provide a better understanding of channel–wetland exchanges and wetland dynamics, and to use the results to improve the parameterisation of a basin-scale model. The study focused on improving the water resource assessments modelling of three data-scarce African river basins that contain large wetlands: the floodplains of the Luangwa and Upper Zambezi River basins and the Usangu wetland in the Upper Great Ruaha River basin. The overall objective was achieved through a combined modelling approach that uses a detailed high-resolution LISFLOOD-FP hydraulic model to inform the structure and parameters of the GW Pitman monthly hydrological model. The results from the LISFLOOD-FP were used to improve the understanding of the channel–wetland exchange dynamics and to establish the wetland parameters required in the GW Pitman model. While some wetland parameters were directly quantified from the LISFLOOD-FP model results, others, which are highly empirical, were estimated by manually calibrating the GW Pitman wetland sub-model implemented in excel spreadsheets containing the LISFLOOD-FP model results. Finally, the GW Pitman model with the inclusion of the estimated wetland parameters was applied for each basin and the results compared to the available downstream observed flow data. The two models have been successfully applied in southern Africa, with the GW Pitman model being one of the most widely applied hydrological models in this region. To address the issue of data scarcity, during setup of these models, the study mainly relied on the global datasets which clearly adds to the overall uncertainty of the modelling approach. However, this is a typical situation for most of the data scarce regions of the continent. A number of challenges were, however, faced during the setup of the LISFLOOD-FP, mainly due to the limitations of the data inputs. Some of the LISFLOOD-FP data inputs include boundary conditions (upstream and downstream), channel cross-sections and wetland topography. In the absence of observed daily flows to quantify the wetland upstream boundary conditions, monthly flow volumes simulated using the GW Pitman monthly model (without including the wetland sub-model) were disaggregated into daily flows using a disaggregation sub-model. The simulated wetland inflows were evaluated using the observed flow data for downstream gauging stations that include the wetland effects. The results highlighted that it is important to understand the possible impacts of each wetland on the downstream flow regime during the evaluations of the model simulation results. Although the disaggregation approach cannot be validated due to a lack of observed data, it at least enables the simulated monthly flows to be used in the daily time step hydraulic model. One of the recommendations is that improvements are required in gauging station networks to provide more observed information for the main river and the larger tributary inflows into these large and important wetland systems. Even a limited amount of newly observed data would be helpful to reduce some of the uncertainties in the combined modelling approach. The SRTM 90 m DEM (used to represent wetland topography) was filtered to reduce local variations and noise effects (mainly vegetation bias), but there were some pixels that falsely affect the inundation results, and the recently released vegetation-corrected DEMs are suggested to improve the simulation results. Channel cross-section values derived from global datasets should be examined because some widths estimated from the Andreadis et al. (2013) dataset were found to be over-generalised and did not reflect widths measured using high-resolution Google Earth in many places. There is an indication that channel cross-sections digitised from Google Earth images can be successfully used in the model setup except in densely vegetated swamps where the values are difficult to estimate, and in such situations, field measured cross-section data are required. Small channels such as those found in the Usangu wetland could play major role in the exchange dynamics, but digitising them all was not straightforward and only key ones were included in the model setup. Clearly, this inevitably introduced uncertainties in the simulated results, and future studies should consider applying methods that simplify extractions of most of these channels from high-resolution images to improve the simulated results. The study demonstrated that the wetland and channel physical characteristics, as well as the seasonal flow magnitude, largely influence the channel–wetland exchanges and wetland dynamics. The inundation results indicated that the area–storage and storage–inflow relationships form hysteretic curves, but the shape of these curves vary with flood magnitude and wetland type. Anticlockwise hysteresis curves were observed in both relationships for the floodplains (Luangwa and Barotse), whereas there appears to be no dominant curve type for the Usangu wetlands. The lack of well-defined hysteretic relationships in the Usangu could be related to some of the difficulties (and resulting uncertainties) that were experienced in setting up the model for this wetland. The storage–inflow relationships in all wetlands have quite complex rising limbs due to multiple flow peaks during the main wet season. The largest inundation area and storage volume for the Barotse and Usangu wetlands occurred after the peak discharge of the wet season, a result that is clearly related to the degree of connectivity between the main channel and those areas of the wetlands that are furthest away from the channel. Hysteresis effects were found to increase with an increase in flood magnitudes and temporal variations in the wetland inflows. Overall, hysteresis behaviour is common in large wetlands and it is recommended that hysteresis curves should be reflected in basin-scale modelling of large river basins with substantial wetland areas. At a daily time scale, inflow–outflow relationships showed a significant peak reduction and a delayed time to peak of several weeks in the Barotse and Usangu wetlands, whereas the attenuation effects of the Luangwa floodplain are minimal. To a large extent, the LISFLOOD-FP results provided useful information to establish wetland parameters and assess the structure of Pitman wetland sub-model. The simple spreadsheet used to estimate wetland parameters did not account for the wetland water transfers from the upstream to the next section downstream (the condition that is included in the LISFLOOD-FP model) for the case when the wetlands were distributed across more than one sub-basin. It is recommended that a method that allows for the upstream wetland inflows and the channel inflows should be included in the spreadsheet. The same is true to the Pitman model structure, and a downstream transfer of water can be modelled through return flows to the channel. The structure of the wetland sub-model was modified to allow an option for the return flows to occur at any time during the simulation period to provide for types of wetlands (e.g. the Luangwa) where spills from the channel and drainage back to the channel occur simultaneously. The setup of the GW Pitman model with the inclusion of wetland parameters improved the simulation results. However, the results for the Usangu wetlands were not very satisfactory and the collection of additional field data related to exchange dynamics is recommended to achieve improvements. The impacts of the Luangwa floodplain on the flow regime of the Luangwa River are very small at the monthly time scale, whereas the Barotse floodplain system and the Usangu wetlands extensively regulate flows of the Zambezi River and the Great Ruaha River, respectively. The results highlighted the possibilities of regionalising some wetland parameters using an understanding of wetland physical characteristics and their water exchange dynamics. However, some parameters remain difficult to quantify in the absence of site-specific information about the water exchange dynamics. The overall conclusion is that the approach implemented in this study presents an important step towards the improvements of water resource assessments modelling for research and practical purposes in data-scarce river basins. This approach is not restricted to the two used models, as it can be applied using different model combinations to achieve similar study purpose.
- Full Text:
- Date Issued: 2020
A combined modelling approach for simulating channel–wetland exchanges in large African river basins
- Authors: Makungu, Eunice J
- Date: 2020
- Subjects: Watersheds -- Africa , Watershed management -- Africa , Water resources development -- Africa -- International cooperation , Floodplain management -- Africa , Wetland ecology -- Simulation methods -- Africa , Wetland management -- Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/123288 , vital:35424
- Description: In Africa, many large and extensive wetlands are hydrologically connected to rivers, and their environmental integrity, as well as their influence on downstream flow regimes, depends on the prevailing channel–wetland exchange processes. These processes are inherently complex and vary spatially and temporally. Understanding channel–wetland exchanges is therefore, indispensable for the effective management of wetlands and the associated river basins. However, this information is limited in most of the river basins containing large wetlands in Africa. Furthermore, it is important to understand the links between upstream and downstream flow regimes and the wetland dynamics themselves, specifically where there are water resource developments that may affect these links (upstream developments), or be affected by them (downstream developments). Hydrological modelling of the entire basin using basin-scale models that include wetland components in their structures can be used to provide the information required to manage water resources in such basins. However, the level of detail of wetland processes included in many basin-scale models is typically very low and the lack of understanding of the wetland dynamics makes it difficult to quantify the relevant parameters. Detailed hydraulic models represent the channel-wetland exchanges in a much more explicit manner, but require relatively more data and time resources to establish than coarser scale hydrological models. The main objective of this study was, therefore, to investigate the use of a detailed hydraulic wetland model to provide a better understanding of channel–wetland exchanges and wetland dynamics, and to use the results to improve the parameterisation of a basin-scale model. The study focused on improving the water resource assessments modelling of three data-scarce African river basins that contain large wetlands: the floodplains of the Luangwa and Upper Zambezi River basins and the Usangu wetland in the Upper Great Ruaha River basin. The overall objective was achieved through a combined modelling approach that uses a detailed high-resolution LISFLOOD-FP hydraulic model to inform the structure and parameters of the GW Pitman monthly hydrological model. The results from the LISFLOOD-FP were used to improve the understanding of the channel–wetland exchange dynamics and to establish the wetland parameters required in the GW Pitman model. While some wetland parameters were directly quantified from the LISFLOOD-FP model results, others, which are highly empirical, were estimated by manually calibrating the GW Pitman wetland sub-model implemented in excel spreadsheets containing the LISFLOOD-FP model results. Finally, the GW Pitman model with the inclusion of the estimated wetland parameters was applied for each basin and the results compared to the available downstream observed flow data. The two models have been successfully applied in southern Africa, with the GW Pitman model being one of the most widely applied hydrological models in this region. To address the issue of data scarcity, during setup of these models, the study mainly relied on the global datasets which clearly adds to the overall uncertainty of the modelling approach. However, this is a typical situation for most of the data scarce regions of the continent. A number of challenges were, however, faced during the setup of the LISFLOOD-FP, mainly due to the limitations of the data inputs. Some of the LISFLOOD-FP data inputs include boundary conditions (upstream and downstream), channel cross-sections and wetland topography. In the absence of observed daily flows to quantify the wetland upstream boundary conditions, monthly flow volumes simulated using the GW Pitman monthly model (without including the wetland sub-model) were disaggregated into daily flows using a disaggregation sub-model. The simulated wetland inflows were evaluated using the observed flow data for downstream gauging stations that include the wetland effects. The results highlighted that it is important to understand the possible impacts of each wetland on the downstream flow regime during the evaluations of the model simulation results. Although the disaggregation approach cannot be validated due to a lack of observed data, it at least enables the simulated monthly flows to be used in the daily time step hydraulic model. One of the recommendations is that improvements are required in gauging station networks to provide more observed information for the main river and the larger tributary inflows into these large and important wetland systems. Even a limited amount of newly observed data would be helpful to reduce some of the uncertainties in the combined modelling approach. The SRTM 90 m DEM (used to represent wetland topography) was filtered to reduce local variations and noise effects (mainly vegetation bias), but there were some pixels that falsely affect the inundation results, and the recently released vegetation-corrected DEMs are suggested to improve the simulation results. Channel cross-section values derived from global datasets should be examined because some widths estimated from the Andreadis et al. (2013) dataset were found to be over-generalised and did not reflect widths measured using high-resolution Google Earth in many places. There is an indication that channel cross-sections digitised from Google Earth images can be successfully used in the model setup except in densely vegetated swamps where the values are difficult to estimate, and in such situations, field measured cross-section data are required. Small channels such as those found in the Usangu wetland could play major role in the exchange dynamics, but digitising them all was not straightforward and only key ones were included in the model setup. Clearly, this inevitably introduced uncertainties in the simulated results, and future studies should consider applying methods that simplify extractions of most of these channels from high-resolution images to improve the simulated results. The study demonstrated that the wetland and channel physical characteristics, as well as the seasonal flow magnitude, largely influence the channel–wetland exchanges and wetland dynamics. The inundation results indicated that the area–storage and storage–inflow relationships form hysteretic curves, but the shape of these curves vary with flood magnitude and wetland type. Anticlockwise hysteresis curves were observed in both relationships for the floodplains (Luangwa and Barotse), whereas there appears to be no dominant curve type for the Usangu wetlands. The lack of well-defined hysteretic relationships in the Usangu could be related to some of the difficulties (and resulting uncertainties) that were experienced in setting up the model for this wetland. The storage–inflow relationships in all wetlands have quite complex rising limbs due to multiple flow peaks during the main wet season. The largest inundation area and storage volume for the Barotse and Usangu wetlands occurred after the peak discharge of the wet season, a result that is clearly related to the degree of connectivity between the main channel and those areas of the wetlands that are furthest away from the channel. Hysteresis effects were found to increase with an increase in flood magnitudes and temporal variations in the wetland inflows. Overall, hysteresis behaviour is common in large wetlands and it is recommended that hysteresis curves should be reflected in basin-scale modelling of large river basins with substantial wetland areas. At a daily time scale, inflow–outflow relationships showed a significant peak reduction and a delayed time to peak of several weeks in the Barotse and Usangu wetlands, whereas the attenuation effects of the Luangwa floodplain are minimal. To a large extent, the LISFLOOD-FP results provided useful information to establish wetland parameters and assess the structure of Pitman wetland sub-model. The simple spreadsheet used to estimate wetland parameters did not account for the wetland water transfers from the upstream to the next section downstream (the condition that is included in the LISFLOOD-FP model) for the case when the wetlands were distributed across more than one sub-basin. It is recommended that a method that allows for the upstream wetland inflows and the channel inflows should be included in the spreadsheet. The same is true to the Pitman model structure, and a downstream transfer of water can be modelled through return flows to the channel. The structure of the wetland sub-model was modified to allow an option for the return flows to occur at any time during the simulation period to provide for types of wetlands (e.g. the Luangwa) where spills from the channel and drainage back to the channel occur simultaneously. The setup of the GW Pitman model with the inclusion of wetland parameters improved the simulation results. However, the results for the Usangu wetlands were not very satisfactory and the collection of additional field data related to exchange dynamics is recommended to achieve improvements. The impacts of the Luangwa floodplain on the flow regime of the Luangwa River are very small at the monthly time scale, whereas the Barotse floodplain system and the Usangu wetlands extensively regulate flows of the Zambezi River and the Great Ruaha River, respectively. The results highlighted the possibilities of regionalising some wetland parameters using an understanding of wetland physical characteristics and their water exchange dynamics. However, some parameters remain difficult to quantify in the absence of site-specific information about the water exchange dynamics. The overall conclusion is that the approach implemented in this study presents an important step towards the improvements of water resource assessments modelling for research and practical purposes in data-scarce river basins. This approach is not restricted to the two used models, as it can be applied using different model combinations to achieve similar study purpose.
- Full Text:
- Date Issued: 2020
Performance, functionalism and form in Ịzọn oral poety
- Authors: Armstrong, Imomotimi
- Date: 2020
- Subjects: Ijo (African people , Ijo language , Folk poetry, Ijo , Folk poetry -- Nigeria
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/140641 , vital:37906
- Description: Since the publication of Ruth Finnegan’s influential Oral Literature in Africa, way back in 1970, scholars have been paying earnest attention to oral traditions on the African continent. That seminal book pointed out to Africanist scholars the need to urgently collect and document the oral literatures of their various ethnic groups before they die out. However, it is the verbal arts of the major ethnic groups on the continent that very often benefit from this collection and documentation, as it were. Therefore, this study sought to examine the oral poetry of the Ịzọn, a minority ethnic nationality, located in the oil-rich Niger Delta region of Nigeria. The study employed unstructured interviews and participant observations to collect the data for the research. The transcribed and translated data was examined through three eclectic theories to the study of folklore: Russian formalism, performance and functionalism. The study found out that Ịzọn oral poetry is a combination of songs and one person’s praise chants. Moreover, it revealed that praise chanting is a recent practice amongst the Ịzọn that was introduced into Ịzọnland by Chief Adolphus Munamuna from the Yoruba of southwestern Nigeria. Furthermore, the study established that oral poetry plays important roles amongst the people. Besides, it ascertained that the performance of some sub-categories of the poetry is highly dramatic and theatrical. It also discovered that stylistic techniques such as formula, parallelism, proverb, ideophone, praise title, metaphor, repetition, alliteration, assonance, vowel lengthening, amongst others, give the poetry the quality of “literariness.” In addition, the study found out that the poetry, like oral poetry in other ethnic groups, demonstrates the three qualities of change, adaptability and survival. The study has contributed to existing scholarship on African oral traditions in the sense of collecting, documenting and generating awareness on Ịzọn oral poetry, most importantly pointing out the existence of praise chanting amongst a people who had no such culture and the conditions that gave rise to that practice.
- Full Text:
- Date Issued: 2020
- Authors: Armstrong, Imomotimi
- Date: 2020
- Subjects: Ijo (African people , Ijo language , Folk poetry, Ijo , Folk poetry -- Nigeria
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/140641 , vital:37906
- Description: Since the publication of Ruth Finnegan’s influential Oral Literature in Africa, way back in 1970, scholars have been paying earnest attention to oral traditions on the African continent. That seminal book pointed out to Africanist scholars the need to urgently collect and document the oral literatures of their various ethnic groups before they die out. However, it is the verbal arts of the major ethnic groups on the continent that very often benefit from this collection and documentation, as it were. Therefore, this study sought to examine the oral poetry of the Ịzọn, a minority ethnic nationality, located in the oil-rich Niger Delta region of Nigeria. The study employed unstructured interviews and participant observations to collect the data for the research. The transcribed and translated data was examined through three eclectic theories to the study of folklore: Russian formalism, performance and functionalism. The study found out that Ịzọn oral poetry is a combination of songs and one person’s praise chants. Moreover, it revealed that praise chanting is a recent practice amongst the Ịzọn that was introduced into Ịzọnland by Chief Adolphus Munamuna from the Yoruba of southwestern Nigeria. Furthermore, the study established that oral poetry plays important roles amongst the people. Besides, it ascertained that the performance of some sub-categories of the poetry is highly dramatic and theatrical. It also discovered that stylistic techniques such as formula, parallelism, proverb, ideophone, praise title, metaphor, repetition, alliteration, assonance, vowel lengthening, amongst others, give the poetry the quality of “literariness.” In addition, the study found out that the poetry, like oral poetry in other ethnic groups, demonstrates the three qualities of change, adaptability and survival. The study has contributed to existing scholarship on African oral traditions in the sense of collecting, documenting and generating awareness on Ịzọn oral poetry, most importantly pointing out the existence of praise chanting amongst a people who had no such culture and the conditions that gave rise to that practice.
- Full Text:
- Date Issued: 2020
Contesting boundaries: a case of municipal demarcation disputes in Vuwani Vhembe District
- Authors: Khowa, Thandeka Promise
- Date: 2020
- Subjects: Geographical boundaries
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/50767 , vital:42670
- Description: The research study in Vuwani Vhembe district sought to investigate demarcation disputes, the relationship between service delivery and demarcation disputes. The study further investigates the role of municipal demarcation board and local government adherence to sound governance principles and the causes of the demarcation dispute in Vuwani. The study additionally examines the conceptions and misconceptions, their impact on the current demarcation disputes in Vuwani. It further intended to establish the role and involvement of Vuwani stakeholders such as traditional structures/ leaders, civil unions, community members etc. in the processes of demarcating Vuwani. “What is the cause of Vuwani community member’s rejection of the re-demarcation, which is said to correct the past spatial injustices and promote municipal sizing, thus enabling better performance in service provision by municipalities?” The study employed a mixed-method involving the use of both quantitative (mini-survey) and qualitative (use of in-depth, semi structures interviews and focus group discussions). Research findings suggest that re-demarcation is a sensitive aspect/process in South Africa, mainly due to the past Apartheid era. There was forceful removal of people from their land supported by Apartheid legislation such as the Native Land Act 1913, Group Act 1950, Native Resettlement Act 1954 etc. Thus, fears of the past Apartheid system cloud any attempt to correct spatial injustices of the country. The new democratic South African government, therefore, needs to be cautious in its acts to correct the spatial injustices, thus adhering to sound governance principles. Findings reveal that several misconceptions and lack of adherence of good governance principles by the Municipal Demarcation Board and Local government have, to some extent, fueled the demarcation disputes in Vuwani. Municipal performance in the rendering of service delivery has a major impact and contributes to the community’s acceptance or rejection of demarcation/ amalgamations. The study further reveals that in Vuwani municipal performance with the establishment of the new Collins Chabane municipality has been clouded by fear of the unknown as this form of “demarcation of establishing a new municipality” altogether is relatively new. Rural land authority, the role of traditional leaders, ethnicity ties have all been found to have contributed to the disputes in Vuwani. Limpopo Province is home to three ethnic groups, namely, vaVhenda, baPedi and xiTsonga. The study findings have presented traits of tribalism as contributing to on-going demarcation disputes in Vuwani.
- Full Text:
- Date Issued: 2020
- Authors: Khowa, Thandeka Promise
- Date: 2020
- Subjects: Geographical boundaries
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/50767 , vital:42670
- Description: The research study in Vuwani Vhembe district sought to investigate demarcation disputes, the relationship between service delivery and demarcation disputes. The study further investigates the role of municipal demarcation board and local government adherence to sound governance principles and the causes of the demarcation dispute in Vuwani. The study additionally examines the conceptions and misconceptions, their impact on the current demarcation disputes in Vuwani. It further intended to establish the role and involvement of Vuwani stakeholders such as traditional structures/ leaders, civil unions, community members etc. in the processes of demarcating Vuwani. “What is the cause of Vuwani community member’s rejection of the re-demarcation, which is said to correct the past spatial injustices and promote municipal sizing, thus enabling better performance in service provision by municipalities?” The study employed a mixed-method involving the use of both quantitative (mini-survey) and qualitative (use of in-depth, semi structures interviews and focus group discussions). Research findings suggest that re-demarcation is a sensitive aspect/process in South Africa, mainly due to the past Apartheid era. There was forceful removal of people from their land supported by Apartheid legislation such as the Native Land Act 1913, Group Act 1950, Native Resettlement Act 1954 etc. Thus, fears of the past Apartheid system cloud any attempt to correct spatial injustices of the country. The new democratic South African government, therefore, needs to be cautious in its acts to correct the spatial injustices, thus adhering to sound governance principles. Findings reveal that several misconceptions and lack of adherence of good governance principles by the Municipal Demarcation Board and Local government have, to some extent, fueled the demarcation disputes in Vuwani. Municipal performance in the rendering of service delivery has a major impact and contributes to the community’s acceptance or rejection of demarcation/ amalgamations. The study further reveals that in Vuwani municipal performance with the establishment of the new Collins Chabane municipality has been clouded by fear of the unknown as this form of “demarcation of establishing a new municipality” altogether is relatively new. Rural land authority, the role of traditional leaders, ethnicity ties have all been found to have contributed to the disputes in Vuwani. Limpopo Province is home to three ethnic groups, namely, vaVhenda, baPedi and xiTsonga. The study findings have presented traits of tribalism as contributing to on-going demarcation disputes in Vuwani.
- Full Text:
- Date Issued: 2020
Practices, dynamics and discourses of cross-racial adoption: the case of Buffalo city municipality in South Africa
- Authors: Nyasha, Kausi
- Date: 2020
- Subjects: Interracial adoption -- South Africa -- Eastern Cape Adoption -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD(Social Work)
- Identifier: http://hdl.handle.net/10353/18009 , vital:42003
- Description: This study investigated the practices, dynamics, and discourses pertaining to crossracial adoption (CRA) in the Buffalo City Municipality (BCM) in South Africa. The researcher made use of qualitative methods to collect and analyse relevant data, which was made from both the in-depth interviews and focus group discussions. The research sample comprised of six adoption social workers, ten adoptive parents and ten adoptees, who were selected by means of purposive non-random sampling. The researcher also made use of observations, in order to that relevant, valuable, and rich information could be obtained. All the data was subsequently analysed by means of thematic analysis. The principal findings of the study revealed that although it has been legal since 1991, the practices of CRA in South Africa continues to be controversial and that, in the vast majority of cases, it entails black children being adopted by white parents. Relevant discourses covered cultural genocide, the best interests of adopted children, racial identity, colour-blindness, acculturation, and racial consciousness. The phenomenon of CRA needed to be evaluated from all of these standpoints, owing to the racial, cultural, and political perceptions which continue to linger after generations of enforced racial segregation in South Africa. It is evident from the findings that perceptions pertaining to race, culture, and politics exert a significant influence on social relations in the caring for and protection of vulnerable children and on family dynamics in adoptive families. It also emerged from the findings that CRA placements have begun to decline significantly, owing to factors such as long and tedious assessment processes, the xviii attitudes of social workers, a general lack of adequate pre- and post-adoption support for adoptive parents, and shortages of adoption social work staff and resources. The researcher endeavoured to assess the roles of adoption social workers within the adoption triad with respect to determining the adoptability of children and matching them with prospective adoptive parents, recruiting and selecting prospective adoptive parents, counselling prospective parents, counselling biological parents, counselling adopted children, and the providing of post-adoption services. It could be concluded from the findings that although the practice of CRA is an efficacious and desirable form of care for children who are in need of protection, despite any present shortcomings in relation to legislation pertaining to the practice, the phenomenon requires considerably more attention from relevant academics and researchers than it has received to date. The overarching conclusion which emerged from the findings was that although the practice of CRA has attracted controversy and denunciations from several racial groups, it has unlimited potential for erasing the racial stigmas which continue to haunt South African society as well as affording permanency care to those children who are in need of it. The study ends with some recommendations as well as a conceptual framework to deal with the issues of the practices, dynamics and discourses of CRA in South Africa.
- Full Text:
- Date Issued: 2020
- Authors: Nyasha, Kausi
- Date: 2020
- Subjects: Interracial adoption -- South Africa -- Eastern Cape Adoption -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD(Social Work)
- Identifier: http://hdl.handle.net/10353/18009 , vital:42003
- Description: This study investigated the practices, dynamics, and discourses pertaining to crossracial adoption (CRA) in the Buffalo City Municipality (BCM) in South Africa. The researcher made use of qualitative methods to collect and analyse relevant data, which was made from both the in-depth interviews and focus group discussions. The research sample comprised of six adoption social workers, ten adoptive parents and ten adoptees, who were selected by means of purposive non-random sampling. The researcher also made use of observations, in order to that relevant, valuable, and rich information could be obtained. All the data was subsequently analysed by means of thematic analysis. The principal findings of the study revealed that although it has been legal since 1991, the practices of CRA in South Africa continues to be controversial and that, in the vast majority of cases, it entails black children being adopted by white parents. Relevant discourses covered cultural genocide, the best interests of adopted children, racial identity, colour-blindness, acculturation, and racial consciousness. The phenomenon of CRA needed to be evaluated from all of these standpoints, owing to the racial, cultural, and political perceptions which continue to linger after generations of enforced racial segregation in South Africa. It is evident from the findings that perceptions pertaining to race, culture, and politics exert a significant influence on social relations in the caring for and protection of vulnerable children and on family dynamics in adoptive families. It also emerged from the findings that CRA placements have begun to decline significantly, owing to factors such as long and tedious assessment processes, the xviii attitudes of social workers, a general lack of adequate pre- and post-adoption support for adoptive parents, and shortages of adoption social work staff and resources. The researcher endeavoured to assess the roles of adoption social workers within the adoption triad with respect to determining the adoptability of children and matching them with prospective adoptive parents, recruiting and selecting prospective adoptive parents, counselling prospective parents, counselling biological parents, counselling adopted children, and the providing of post-adoption services. It could be concluded from the findings that although the practice of CRA is an efficacious and desirable form of care for children who are in need of protection, despite any present shortcomings in relation to legislation pertaining to the practice, the phenomenon requires considerably more attention from relevant academics and researchers than it has received to date. The overarching conclusion which emerged from the findings was that although the practice of CRA has attracted controversy and denunciations from several racial groups, it has unlimited potential for erasing the racial stigmas which continue to haunt South African society as well as affording permanency care to those children who are in need of it. The study ends with some recommendations as well as a conceptual framework to deal with the issues of the practices, dynamics and discourses of CRA in South Africa.
- Full Text:
- Date Issued: 2020
The precarious ‘good mother’ position: a psychosocial reading of maternal subjectivity of working mothers in scarcely-resourced South African communities
- Authors: Kinahan-Sweeney, Siobhan
- Date: 2020
- Subjects: Motherhood -- Psychological aspects -- South Africa , Working mothers -- South Africa -- Attitudes , Working mothers -- South Africa -- Psychology
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142464 , vital:38082
- Description: This psychosocial study investigates the maternal subjectivities of mothers returning to work after maternity leave, who are living in scarcely-resourced Cape Town communities in South Africa. Engaging with interview texts and listening to mothers’ talk, I explore how and why maternal subjectivity is constructed discursively and defensively in our talk. This thesis claims that these particular mothers predominately employ instrumental mothering discourse. The traditional subject position of the intensive mother – which is typically assumed to be the ‘good mother’ – is not a position available to these mothers due to their social circumstances and working role. Subsequently, material provision, the baby’s thriving and surviving body, finding substitute carers and maternal preoccupation are constructed as qualities of ‘good mothering’ in their talk. This ‘good mother’ position, however, is a precarious position that both these mothers and I invest in to defend against feelings towards their babies and themselves as well as to deny (maternal) ambivalence in a problematic social system. In a combined analysis drawing on discursive theory and psychoanalysis, more specifically contemporary attachment theory and intersubjectivity theory, I illustrate how both these mothers and I – as emotional, social and political subjects – co-construct maternal subjectivity. Based on the findings, recommendations for parent-infant interventions are discussed. Arguing that a purely psychoanalytic reading of investment perpetuates notions of individual blame and pathology, I advocate for a psychosocial reading that does not neglect failing social systems but rather pursues an open and reflective, yet critical, mindfulness when listening to talk.
- Full Text:
- Date Issued: 2020
- Authors: Kinahan-Sweeney, Siobhan
- Date: 2020
- Subjects: Motherhood -- Psychological aspects -- South Africa , Working mothers -- South Africa -- Attitudes , Working mothers -- South Africa -- Psychology
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142464 , vital:38082
- Description: This psychosocial study investigates the maternal subjectivities of mothers returning to work after maternity leave, who are living in scarcely-resourced Cape Town communities in South Africa. Engaging with interview texts and listening to mothers’ talk, I explore how and why maternal subjectivity is constructed discursively and defensively in our talk. This thesis claims that these particular mothers predominately employ instrumental mothering discourse. The traditional subject position of the intensive mother – which is typically assumed to be the ‘good mother’ – is not a position available to these mothers due to their social circumstances and working role. Subsequently, material provision, the baby’s thriving and surviving body, finding substitute carers and maternal preoccupation are constructed as qualities of ‘good mothering’ in their talk. This ‘good mother’ position, however, is a precarious position that both these mothers and I invest in to defend against feelings towards their babies and themselves as well as to deny (maternal) ambivalence in a problematic social system. In a combined analysis drawing on discursive theory and psychoanalysis, more specifically contemporary attachment theory and intersubjectivity theory, I illustrate how both these mothers and I – as emotional, social and political subjects – co-construct maternal subjectivity. Based on the findings, recommendations for parent-infant interventions are discussed. Arguing that a purely psychoanalytic reading of investment perpetuates notions of individual blame and pathology, I advocate for a psychosocial reading that does not neglect failing social systems but rather pursues an open and reflective, yet critical, mindfulness when listening to talk.
- Full Text:
- Date Issued: 2020
A holistic approach in understanding the effects of dietary protein sources on the growth and reproductive development of farmed abalone, Haliotis midae
- Wu, Yu
- Authors: Wu, Yu
- Date: 2020
- Subjects: Abalones -- Nutrition , Abalones -- Reproduction , Abalones -- Growth , Abalone culture , Haliotis midae -- Nutrition , Haliotis midae -- Reproduction , Haliotis midae -- Growth , Haliotis midae fisheries
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167719 , vital:41506
- Description: The combination of fishmeal and soya in the formulated feed of farmed South African abalone, Haliotis midae, not only improved abalone growth, but also the sustainability of the compound diets by reducing the reliance on fishmeal. However, the presence of soya produced larger gonads compared to those of abalone fed kelp or single-source protein diets. There is an increasing drive to control sexual maturation and reduce undesirable spawning events in farmed abalone. However, the reasons for the reported effects of soya inclusion on the reproductive development of farmed H. midae remain unresolved. The aim of this research was to use a combination of techniques to contribute to the understanding of the relationship between growth, reproductive and nutritional physiology of farmed H. midae fed diets of varying protein sources. These techniques included stable isotope analysis (SIA), fatty acid analysis, gonad histological assessments and haemolymph sexsteroid hormone analysis. The objectives were to examine the allocation of protein and nutritional components to somatic and reproductive tissues. This study also included the first attempt to investigate the role that sex steroid hormones play during gametogenesis. Abalone (40 – 50 g abalone-1) were fed one of four diets: either a single-source protein-based diet, i.e., (1) fishmeal-only (FM) or (2) soya-only (S), or a combination diet of (3) fishmeal-soya (FM S) or (4) fishmeal-sunflower meal (FM SM). Sampling occurred in 45-day intervals over one year. Dietary protein source had an effect on the growth of abalone, with enhancements in growth linked to the combination of fishmeal and a plant-protein source. The fishmeal ingredient was the most utilised protein source throughout the temporal changes in protein allocation into somatic and reproductive tissue, followed by soya and then sunflower meal. The mean whole- body mass of abalone was significantly influenced by an interaction between time and dietary treatment, with average whole-body mass changing differently over time between treatments (RM-ANOVA, F(24, 96) = 2.13, p = 0.005). Overall, abalone that were fed FM S had higher mean whole-body mass values than abalone that were fed the single-protein based diets, while abalone that were fed FM SM were similar to animals from all dietary treatments (RMANOVA, F(3, 12) = 5.75, p = 0.01). Male abalone had significantly higher gonad bulk index (GBI) values compared to females (RM-ANOVA, F(1, 24) = 49.03, p < 0.001) and this was independent of dietary treatment. Within each sex, female abalone fed the FM S diet (15.92 ± 1.88 mm3 g-1) had significantly higher GBI values than abalone fed S (9.76 ± 1.08 mm3 g-1), while abalone fed FM (11.96 ± 1.71 mm3 g-1) and FM SM (11.90 ± 0.80 mm3 g-1) were similar to abalone from all other dietary treatments (Tukey’s HSD, p < 0.05). Male abalone fed the FM S (21.59 ± 2.10 mm3 g-1) and FM SM diet (19.30 ± 2.63 mm3 g-1) had similar GBI values and they were significantly higher than in abalone fed the S diet (14.74 ± 1.27 mm3 g-1), while abalone fed FM S had significantly higher GBI values than abalone that consumed the FM diet (15.08 ±1.63mm3 g-1) ( Tukey’s HSD, p < 0.05). Although sunflower meal was poorly utilised, it produced similar overall growth to abalone that were fed the FM S diets. Yet, feed conversion ratio values were significantly lower for abalone fed FM S (1.30 ± 0.13) compared to those in the other three treatments, with ratios ranging from 1.65 – 1.72 over the one-year. The fatty acid compositions of the somatic and gonadal tissues were similar between treatments (PERMANOVA, p = 0.21), while fatty acid composition was influenced by sampling day, tissue type and abalone sex (PERMANOVA, p < 0.05). The essential fatty acids (EFAs) eicosadienoic acid and a-linolenic acid were present in abalone tissue, but they were not detected in the diets, suggesting the important role that an alternate food source (e.g. farmed abalone also had access to diatoms) may have played and the ability that H. midae may have in converting long-chain polyunsaturated fatty acids from C18 precursors. A change in the abundance of EFAs in the gonad tissue during highest and lowest GBI values suggested that arachidonic, eicosapentaenoic, g-linolenic and linoleic acid were important in females, while eicosapentaenoic, eicosadienoic and a-linolenic acid were important for male reproductive development. Dietary protein sources had an effect on the frequency distribution of maturity stages, where females that were fed FM S produced more ripe gonads and more males that were fed FM SM contained testes that showed signs of ripeness over the one-year study. Although dietary protein influenced the sex steroid concentrations in females and males, exhibiting fluctuations throughout the one-year period, no distinct pattern linked to gametogenesis were observed. The results from this study illustrate: (1) the importance of conducting laboratory studies when implementing SIA and mixing models in aquaculture nutrition; (2) conducting nutritional studies on mature, grow-out abalone; and (3) assessing the importance of naturally occurring diatoms in their diet and their contribution to growth and reproduction. The novel contribution of this research towards abalone nutritional physiology, the implications of these findings to industry as well as potential considerations for future studies were addressed.
- Full Text:
- Date Issued: 2020
- Authors: Wu, Yu
- Date: 2020
- Subjects: Abalones -- Nutrition , Abalones -- Reproduction , Abalones -- Growth , Abalone culture , Haliotis midae -- Nutrition , Haliotis midae -- Reproduction , Haliotis midae -- Growth , Haliotis midae fisheries
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167719 , vital:41506
- Description: The combination of fishmeal and soya in the formulated feed of farmed South African abalone, Haliotis midae, not only improved abalone growth, but also the sustainability of the compound diets by reducing the reliance on fishmeal. However, the presence of soya produced larger gonads compared to those of abalone fed kelp or single-source protein diets. There is an increasing drive to control sexual maturation and reduce undesirable spawning events in farmed abalone. However, the reasons for the reported effects of soya inclusion on the reproductive development of farmed H. midae remain unresolved. The aim of this research was to use a combination of techniques to contribute to the understanding of the relationship between growth, reproductive and nutritional physiology of farmed H. midae fed diets of varying protein sources. These techniques included stable isotope analysis (SIA), fatty acid analysis, gonad histological assessments and haemolymph sexsteroid hormone analysis. The objectives were to examine the allocation of protein and nutritional components to somatic and reproductive tissues. This study also included the first attempt to investigate the role that sex steroid hormones play during gametogenesis. Abalone (40 – 50 g abalone-1) were fed one of four diets: either a single-source protein-based diet, i.e., (1) fishmeal-only (FM) or (2) soya-only (S), or a combination diet of (3) fishmeal-soya (FM S) or (4) fishmeal-sunflower meal (FM SM). Sampling occurred in 45-day intervals over one year. Dietary protein source had an effect on the growth of abalone, with enhancements in growth linked to the combination of fishmeal and a plant-protein source. The fishmeal ingredient was the most utilised protein source throughout the temporal changes in protein allocation into somatic and reproductive tissue, followed by soya and then sunflower meal. The mean whole- body mass of abalone was significantly influenced by an interaction between time and dietary treatment, with average whole-body mass changing differently over time between treatments (RM-ANOVA, F(24, 96) = 2.13, p = 0.005). Overall, abalone that were fed FM S had higher mean whole-body mass values than abalone that were fed the single-protein based diets, while abalone that were fed FM SM were similar to animals from all dietary treatments (RMANOVA, F(3, 12) = 5.75, p = 0.01). Male abalone had significantly higher gonad bulk index (GBI) values compared to females (RM-ANOVA, F(1, 24) = 49.03, p < 0.001) and this was independent of dietary treatment. Within each sex, female abalone fed the FM S diet (15.92 ± 1.88 mm3 g-1) had significantly higher GBI values than abalone fed S (9.76 ± 1.08 mm3 g-1), while abalone fed FM (11.96 ± 1.71 mm3 g-1) and FM SM (11.90 ± 0.80 mm3 g-1) were similar to abalone from all other dietary treatments (Tukey’s HSD, p < 0.05). Male abalone fed the FM S (21.59 ± 2.10 mm3 g-1) and FM SM diet (19.30 ± 2.63 mm3 g-1) had similar GBI values and they were significantly higher than in abalone fed the S diet (14.74 ± 1.27 mm3 g-1), while abalone fed FM S had significantly higher GBI values than abalone that consumed the FM diet (15.08 ±1.63mm3 g-1) ( Tukey’s HSD, p < 0.05). Although sunflower meal was poorly utilised, it produced similar overall growth to abalone that were fed the FM S diets. Yet, feed conversion ratio values were significantly lower for abalone fed FM S (1.30 ± 0.13) compared to those in the other three treatments, with ratios ranging from 1.65 – 1.72 over the one-year. The fatty acid compositions of the somatic and gonadal tissues were similar between treatments (PERMANOVA, p = 0.21), while fatty acid composition was influenced by sampling day, tissue type and abalone sex (PERMANOVA, p < 0.05). The essential fatty acids (EFAs) eicosadienoic acid and a-linolenic acid were present in abalone tissue, but they were not detected in the diets, suggesting the important role that an alternate food source (e.g. farmed abalone also had access to diatoms) may have played and the ability that H. midae may have in converting long-chain polyunsaturated fatty acids from C18 precursors. A change in the abundance of EFAs in the gonad tissue during highest and lowest GBI values suggested that arachidonic, eicosapentaenoic, g-linolenic and linoleic acid were important in females, while eicosapentaenoic, eicosadienoic and a-linolenic acid were important for male reproductive development. Dietary protein sources had an effect on the frequency distribution of maturity stages, where females that were fed FM S produced more ripe gonads and more males that were fed FM SM contained testes that showed signs of ripeness over the one-year study. Although dietary protein influenced the sex steroid concentrations in females and males, exhibiting fluctuations throughout the one-year period, no distinct pattern linked to gametogenesis were observed. The results from this study illustrate: (1) the importance of conducting laboratory studies when implementing SIA and mixing models in aquaculture nutrition; (2) conducting nutritional studies on mature, grow-out abalone; and (3) assessing the importance of naturally occurring diatoms in their diet and their contribution to growth and reproduction. The novel contribution of this research towards abalone nutritional physiology, the implications of these findings to industry as well as potential considerations for future studies were addressed.
- Full Text:
- Date Issued: 2020
Phytoplankton of the Southern Agulhas Large Marine Ecosystem (sACLME)
- Authors: Sonnekus, Martinus Jakobus
- Date: 2020
- Subjects: Botanical chemistry
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/47646 , vital:40261
- Description: The southern Agulhas Large Marine Ecosystem (sACLME) consists of the greater Agulhas Current system, including the areas influenced by the Agulhas Current, the Agulhas Shelf, Agulhas Bank, Agulhas Retroflection as well as the Agulhas Return Current. Four biogeographical regions were identified within the sACLME and the composition of the phytoplankton communities and the associated physico-chemical variables in each were investigated. Water temperature and nitrate concentration were found to be the main drivers of the sACLME phytoplankton community. A total of 215 phytoplankton taxa were identified that were separated into two groups: the Agulhas Current group and the Southwest Indian Ocean Ridge group. The structure of the phytoplankton community was consistently homogeneous throughout the sACLME. Nutrient concentrations of the water varied, but nitrate was the most abundant source of nitrogen, especially in regions that were subjected to the upwelling of cold nutrient-rich water. The phytoplankton communities were significantly influenced by the availability and stoichiometry of the macronutrients nitrogen; phosphorus and silicon. The low mean N:P ratio of 5.5 is an indication that the waters of the sACLME are generally nitrogen limited, as is typical of oceanic systems. There is a change in phytoplankton cell size and functional groups within in the various water masses that are specific to certain biogeographical regions within the Agulhas Current system. It is clear that frontal systems may form barriers between phytoplankton communities.
- Full Text:
- Date Issued: 2020
- Authors: Sonnekus, Martinus Jakobus
- Date: 2020
- Subjects: Botanical chemistry
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/47646 , vital:40261
- Description: The southern Agulhas Large Marine Ecosystem (sACLME) consists of the greater Agulhas Current system, including the areas influenced by the Agulhas Current, the Agulhas Shelf, Agulhas Bank, Agulhas Retroflection as well as the Agulhas Return Current. Four biogeographical regions were identified within the sACLME and the composition of the phytoplankton communities and the associated physico-chemical variables in each were investigated. Water temperature and nitrate concentration were found to be the main drivers of the sACLME phytoplankton community. A total of 215 phytoplankton taxa were identified that were separated into two groups: the Agulhas Current group and the Southwest Indian Ocean Ridge group. The structure of the phytoplankton community was consistently homogeneous throughout the sACLME. Nutrient concentrations of the water varied, but nitrate was the most abundant source of nitrogen, especially in regions that were subjected to the upwelling of cold nutrient-rich water. The phytoplankton communities were significantly influenced by the availability and stoichiometry of the macronutrients nitrogen; phosphorus and silicon. The low mean N:P ratio of 5.5 is an indication that the waters of the sACLME are generally nitrogen limited, as is typical of oceanic systems. There is a change in phytoplankton cell size and functional groups within in the various water masses that are specific to certain biogeographical regions within the Agulhas Current system. It is clear that frontal systems may form barriers between phytoplankton communities.
- Full Text:
- Date Issued: 2020
A strategy towards implementing standardised data structures in municipal information systems
- Authors: Koekemoer, Silma Marguerite
- Date: 2020
- Subjects: Data structures (Computer science) , Municipal government -- Data processing Public administration -- Data processing Management information systems
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/46064 , vital:39482
- Description: The regulation pertaining to a Standard Chart of Accounts (SCOA) for Municipalities was published in 2014 and is applicable to all 257 municipalities and municipal entities in South Africa. The regulation represents a data classification framework or structure and affects all financial management and internal control systems (FMICS) used by municipalities and municipal entities, and affects key business processes within these organisations. Compliance with the SCOA regulation means that the full municipal accountability cycle should accommodate all seven of the SCOA segments, from the budget through transacting and reporting at the transaction level, with all seven of the segments being embedded in the master data table of the municipal FMICS. While the change to technology and systems may be self-evident, the related business change should not be underestimated. This information technology (IT) driven organisational change across the whole municipal environment represents the research topic and key objective of this research study, namely, a strategy towards implementing standard data structures in municipal IT systems. The study followed a pragmatic philosophy using diagnostic reasoning based on an inductive approach, multiple action research methods and a descriptive case study to derive the proposed implementation strategy. The research subjects, which included 25 pilot municipalities, were studied for the duration of the pilot implementation of the strategy with the objective of identifying and utilising the lessons learnt from their experience to fast track the rollout of the strategy to non-pilot municipalities. The study was limited to the local government environment and to South Africa as a geographic area and involved an accidental sample aligned to the implementation project under investigation. The proposed implementation strategy was, however, of a generic nature and is therefore applicable to any other institution or environment engaged in a similar implementation project. The main contribution of the study is an implementation strategy for standard data structures in municipal financial information systems and which consists of seven diagnostics, 17 guiding policies and 48 coherent actions. The strategy was developed and refined during six cycles of data collection, which were conducted at 25 municipalities actively involved in implementing of the standard data structures. The secondary contributions of the research study include three conference papers and one submission to an academic journal.
- Full Text:
- Date Issued: 2020
- Authors: Koekemoer, Silma Marguerite
- Date: 2020
- Subjects: Data structures (Computer science) , Municipal government -- Data processing Public administration -- Data processing Management information systems
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/46064 , vital:39482
- Description: The regulation pertaining to a Standard Chart of Accounts (SCOA) for Municipalities was published in 2014 and is applicable to all 257 municipalities and municipal entities in South Africa. The regulation represents a data classification framework or structure and affects all financial management and internal control systems (FMICS) used by municipalities and municipal entities, and affects key business processes within these organisations. Compliance with the SCOA regulation means that the full municipal accountability cycle should accommodate all seven of the SCOA segments, from the budget through transacting and reporting at the transaction level, with all seven of the segments being embedded in the master data table of the municipal FMICS. While the change to technology and systems may be self-evident, the related business change should not be underestimated. This information technology (IT) driven organisational change across the whole municipal environment represents the research topic and key objective of this research study, namely, a strategy towards implementing standard data structures in municipal IT systems. The study followed a pragmatic philosophy using diagnostic reasoning based on an inductive approach, multiple action research methods and a descriptive case study to derive the proposed implementation strategy. The research subjects, which included 25 pilot municipalities, were studied for the duration of the pilot implementation of the strategy with the objective of identifying and utilising the lessons learnt from their experience to fast track the rollout of the strategy to non-pilot municipalities. The study was limited to the local government environment and to South Africa as a geographic area and involved an accidental sample aligned to the implementation project under investigation. The proposed implementation strategy was, however, of a generic nature and is therefore applicable to any other institution or environment engaged in a similar implementation project. The main contribution of the study is an implementation strategy for standard data structures in municipal financial information systems and which consists of seven diagnostics, 17 guiding policies and 48 coherent actions. The strategy was developed and refined during six cycles of data collection, which were conducted at 25 municipalities actively involved in implementing of the standard data structures. The secondary contributions of the research study include three conference papers and one submission to an academic journal.
- Full Text:
- Date Issued: 2020
‘Implementation Strategies Used by Teachers’ Colleges to Prepare Pre-Service Teachers for Science, Technology, Engineering and Mathematics Education in Harare Metropolitan Province in Zimbabwe
- Authors: Chimwe, Ananias
- Date: 2020
- Subjects: Teachers -- In-service training -- Zimbabwe Teachers -- Education (Primary) -- Zimbabwe Science|xStudy and teaching (Primary) -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD (Education)
- Identifier: http://hdl.handle.net/10353/17931 , vital:41981
- Description: The study was carried to establish the strategies used by teacher education colleges to prepare pre-service teachers for STEM Education in Zimbabwe. Invariably, the nature of research questions led the study to be located within the pragmatic paradigm. A mixed method approach and concurrent triangulation design was adopted to examine issues under study. The study adopted stratified random sampling and purposive sampling methods to identify its respondents and participants. Data were collected from respondents who were envisaged knowledgeable about critical issues under study. Several research instruments were used to solicit quantitative and qualitative data. These included: questionnaires, interview schedules, focus group discussions and documents. The sample of the study consisted of 20 lecturers, 50 pre-service teachers, 3 Department of Teacher Education lecturers and 2 Directors in the Ministry of Higher and Tertiary Education Science Innovation, Technology and Development. The study established that 95%of the teacher educators had the requisite STEM content knowledge. Pedagogical content knowledge, knowledge of organisation and education purpose, engineering content and pedagogical content was found lacking. Furthermore, the teacher educators employed 21st century STEM specific inquiry based and constructivist teaching strategies. More so, it was established that government, the Department of Teacher Education and other development partners rendered support to teacher education colleges to prepare for pre-service teachers for STEM education. In addition, the study revealed that preparation of pre-service teachers for STEM was impeded by several structural factors that obtained in teacher education colleges. Overall, the study concluded that teacher educators had requisite STEM knowledge and employed inquiry-based strategies to prepare pre-service teachers for STEM Education. Furthermore, the study recommended that teacher educators’ knowledge in engineering needs further strengthening through workshops and synergies with industry. An alternative model for effective STEM preparation was recommended for consideration.
- Full Text:
- Date Issued: 2020
- Authors: Chimwe, Ananias
- Date: 2020
- Subjects: Teachers -- In-service training -- Zimbabwe Teachers -- Education (Primary) -- Zimbabwe Science|xStudy and teaching (Primary) -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD (Education)
- Identifier: http://hdl.handle.net/10353/17931 , vital:41981
- Description: The study was carried to establish the strategies used by teacher education colleges to prepare pre-service teachers for STEM Education in Zimbabwe. Invariably, the nature of research questions led the study to be located within the pragmatic paradigm. A mixed method approach and concurrent triangulation design was adopted to examine issues under study. The study adopted stratified random sampling and purposive sampling methods to identify its respondents and participants. Data were collected from respondents who were envisaged knowledgeable about critical issues under study. Several research instruments were used to solicit quantitative and qualitative data. These included: questionnaires, interview schedules, focus group discussions and documents. The sample of the study consisted of 20 lecturers, 50 pre-service teachers, 3 Department of Teacher Education lecturers and 2 Directors in the Ministry of Higher and Tertiary Education Science Innovation, Technology and Development. The study established that 95%of the teacher educators had the requisite STEM content knowledge. Pedagogical content knowledge, knowledge of organisation and education purpose, engineering content and pedagogical content was found lacking. Furthermore, the teacher educators employed 21st century STEM specific inquiry based and constructivist teaching strategies. More so, it was established that government, the Department of Teacher Education and other development partners rendered support to teacher education colleges to prepare for pre-service teachers for STEM education. In addition, the study revealed that preparation of pre-service teachers for STEM was impeded by several structural factors that obtained in teacher education colleges. Overall, the study concluded that teacher educators had requisite STEM knowledge and employed inquiry-based strategies to prepare pre-service teachers for STEM Education. Furthermore, the study recommended that teacher educators’ knowledge in engineering needs further strengthening through workshops and synergies with industry. An alternative model for effective STEM preparation was recommended for consideration.
- Full Text:
- Date Issued: 2020