The effect of extracellular Hsp90β and TGF-β1 on colon cancer biology
- Authors: Perks, Tamarin
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/55896 , vital:26753
- Description: The TGF-β signaling pathway is known to be one of the most commonly mutated pathways in human cancers, while Hsp90 is a bone fide drug target that is involved in regulating the conformation and activity of many oncoproteins. The role of intracellular Hsp90 in cancer has thus far been established and there is a growing link between extracellular Hsp90 and cancer metastasis, as well as the role of TGF-β in metastasis. This study aimed to analyse the interaction between Hsp90 (both intracellular and extracellular) and the TGF-β machinery in cancer cells, as well as to determine the effect of these proteins on cellular responses on the biology of cancer cells. This was achieved by studying the expression of Hsp90; TGF-βRII and TGF-β1 in cancer cell lines of various origins using flow cytometry, ELISA, and western blot analysis. The genetically paired SW480 and SW620 colon cancer cell lines, derived from a primary tumour and lymph node metastasis, respectively, were selected for further study due to differences in expression levels and activation of the TGF-β1 pathway. SW480 cells expressed double the level of TGF-βRII compared to SW620 cells, while SW620 expressed two times more extracellular TGF-β1 than SW480 cells. A direct interaction between TGF-β1 and Hsp90β was determined in vitro, and confirmed in vivo in SW620 cells. Growth, adhesion and migration were analysed in SW480 and SW620 cells. SW480 cells adhered significantly faster than SW620 cells, while SW620 cells had a greater rate of migration. Inhibiting the TGF-β pathway, specifically TGF-βRI, using SB 431542, as well as inhibiting Hsp90 with novobiocin, caused an increase in migration in SW480 cells. Only the addition of TGF-β1 in combination with Hsp90 as well as SB 431542 caused an increase in migration in SW620 cells. The canonical TGF-β1/TGF-βRI/TGF-βRII pathway may be constitutively active in SW620 cells and the inhibition of TGF-βRI may suggest an alternate pathway or receptor in both SW480 and SW620 cells.
- Full Text:
- Date Issued: 2015
- Authors: Perks, Tamarin
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/55896 , vital:26753
- Description: The TGF-β signaling pathway is known to be one of the most commonly mutated pathways in human cancers, while Hsp90 is a bone fide drug target that is involved in regulating the conformation and activity of many oncoproteins. The role of intracellular Hsp90 in cancer has thus far been established and there is a growing link between extracellular Hsp90 and cancer metastasis, as well as the role of TGF-β in metastasis. This study aimed to analyse the interaction between Hsp90 (both intracellular and extracellular) and the TGF-β machinery in cancer cells, as well as to determine the effect of these proteins on cellular responses on the biology of cancer cells. This was achieved by studying the expression of Hsp90; TGF-βRII and TGF-β1 in cancer cell lines of various origins using flow cytometry, ELISA, and western blot analysis. The genetically paired SW480 and SW620 colon cancer cell lines, derived from a primary tumour and lymph node metastasis, respectively, were selected for further study due to differences in expression levels and activation of the TGF-β1 pathway. SW480 cells expressed double the level of TGF-βRII compared to SW620 cells, while SW620 expressed two times more extracellular TGF-β1 than SW480 cells. A direct interaction between TGF-β1 and Hsp90β was determined in vitro, and confirmed in vivo in SW620 cells. Growth, adhesion and migration were analysed in SW480 and SW620 cells. SW480 cells adhered significantly faster than SW620 cells, while SW620 cells had a greater rate of migration. Inhibiting the TGF-β pathway, specifically TGF-βRI, using SB 431542, as well as inhibiting Hsp90 with novobiocin, caused an increase in migration in SW480 cells. Only the addition of TGF-β1 in combination with Hsp90 as well as SB 431542 caused an increase in migration in SW620 cells. The canonical TGF-β1/TGF-βRI/TGF-βRII pathway may be constitutively active in SW620 cells and the inhibition of TGF-βRI may suggest an alternate pathway or receptor in both SW480 and SW620 cells.
- Full Text:
- Date Issued: 2015
Bush encroachment in the semi-arid communal grazing lands of Eastern Cape and farmer's perception of causes and livelihood impacts
- Authors: Libala, Notiswa
- Date: 2015
- Subjects: Communal rangelands--South Africa--Eastern Cape Grazing--South Africa--Eastern Cape egetation management--South Africa--Eastern Cape
- Language: English
- Type: Thesis , Masters , Pasture Science
- Identifier: http://hdl.handle.net/10353/11817 , vital:39109
- Description: The study was conducted in two communal rangelands of the Eastern Cape Province (Thorn and Mixed bush veld). The objective of the study was to investigate 1) the historical trends of bush encroachment, causes and impacts based on indigenous knowledge and perceptions of the communal people in two savannah vegetation types and 2) to determine the density and cover of woody species in the grazing areas. In each veld type, two villages were selected purposefully. A total of 120 households who own livestock were randomly selected from the four communal areas and a total of 48 elders were selected to provide information on bush encroachment and rangeland condition. For woody density and cover, grazing areas surrounding two homesteads (settlements) were selected from each communal rangeland, Two long transects radiating in the opposite direction from each homestead were established. The woody vegetation survey was conducted at 100, 200, 300, 400, 500, 600, 700, 800 and 900m distance from each homestead. In each distance, 3 (10 m x 10 m) quadrates were laid to record individual woody plant species, the height, basal stem and canopy diameters. Woody species were classified into seedlings (>0 - 1 m), saplings (>1 - 1.5 m), young shrubs (>1.5 - 2.0 m), mature shrubs (>2 - 3.0 m) and mature trees (>3.0 m). The average household size in of goat was higher than that of other livestock species. A total of 40 woody plant species were identified in all study areas. Acacia karoo, Coddia rudis and Scutia myrtina were the most dominant woody species in all study areas. Most of the woody plant species had the highest abundance in the height class >0 - 1 m. The average total woody plant density in Thorn bush and Mixed bush were 3461 and 2416 number ha-1, respectively. In conclusion both study areas were highly encroached and it was also perceived by elder from both areas that bush encroachment is a problem in the rangelands.
- Full Text:
- Date Issued: 2015
- Authors: Libala, Notiswa
- Date: 2015
- Subjects: Communal rangelands--South Africa--Eastern Cape Grazing--South Africa--Eastern Cape egetation management--South Africa--Eastern Cape
- Language: English
- Type: Thesis , Masters , Pasture Science
- Identifier: http://hdl.handle.net/10353/11817 , vital:39109
- Description: The study was conducted in two communal rangelands of the Eastern Cape Province (Thorn and Mixed bush veld). The objective of the study was to investigate 1) the historical trends of bush encroachment, causes and impacts based on indigenous knowledge and perceptions of the communal people in two savannah vegetation types and 2) to determine the density and cover of woody species in the grazing areas. In each veld type, two villages were selected purposefully. A total of 120 households who own livestock were randomly selected from the four communal areas and a total of 48 elders were selected to provide information on bush encroachment and rangeland condition. For woody density and cover, grazing areas surrounding two homesteads (settlements) were selected from each communal rangeland, Two long transects radiating in the opposite direction from each homestead were established. The woody vegetation survey was conducted at 100, 200, 300, 400, 500, 600, 700, 800 and 900m distance from each homestead. In each distance, 3 (10 m x 10 m) quadrates were laid to record individual woody plant species, the height, basal stem and canopy diameters. Woody species were classified into seedlings (>0 - 1 m), saplings (>1 - 1.5 m), young shrubs (>1.5 - 2.0 m), mature shrubs (>2 - 3.0 m) and mature trees (>3.0 m). The average household size in of goat was higher than that of other livestock species. A total of 40 woody plant species were identified in all study areas. Acacia karoo, Coddia rudis and Scutia myrtina were the most dominant woody species in all study areas. Most of the woody plant species had the highest abundance in the height class >0 - 1 m. The average total woody plant density in Thorn bush and Mixed bush were 3461 and 2416 number ha-1, respectively. In conclusion both study areas were highly encroached and it was also perceived by elder from both areas that bush encroachment is a problem in the rangelands.
- Full Text:
- Date Issued: 2015
Film as an agent of cultural transmission : a study of Nollywood films
- Onyenankeya, Oluwayemisi Mary
- Authors: Onyenankeya, Oluwayemisi Mary
- Date: 2015
- Subjects: Video recordings--Social aspects--Nigeria Motion pictures--Africa, West Digital video--Production and direction--Nigeria
- Language: English
- Type: Thesis , Masters , Communication
- Identifier: http://hdl.handle.net/10353/11685 , vital:39096
- Description: The popularity and viewership of Nollywood films have expanded across African countries as well as European and American cities especially those with considerable Diaspora population. This appears to underscore the assumption that Nollywood films not only project or transmit the embedded Nigerian cultural values to audience members but also actually make appreciable impression on those audience members. This study sought to ascertain the role of Nollywood film in the transference of Nigerian cultural heritage across other cultures using students at the University of Fort Hare as a case study. This study sought to determine if audience members learn, imbibe or manifest any behavioural tendencies that can be ascribed to the influence of watching Nollywood films. Specifically the study sought to establish the aspect of Nigerian culture that Nollywood films transmit to audience members. The survey research method was used while data was analysed using descriptive statistics. In all, 400 questionnaires were distributed while 370 were returned. Results show that students who were exposed to Nollywood film could mimic some common Nigerian expressions and exclamations. A small percentage of viewers could actually speak a smattering of some Nigerian language while a few have bought or made dresses in the pattern of what they saw in the films. It could be concluded that Nollywood films do exert significant influence on the viewers in the University of Fort Hare.
- Full Text:
- Date Issued: 2015
- Authors: Onyenankeya, Oluwayemisi Mary
- Date: 2015
- Subjects: Video recordings--Social aspects--Nigeria Motion pictures--Africa, West Digital video--Production and direction--Nigeria
- Language: English
- Type: Thesis , Masters , Communication
- Identifier: http://hdl.handle.net/10353/11685 , vital:39096
- Description: The popularity and viewership of Nollywood films have expanded across African countries as well as European and American cities especially those with considerable Diaspora population. This appears to underscore the assumption that Nollywood films not only project or transmit the embedded Nigerian cultural values to audience members but also actually make appreciable impression on those audience members. This study sought to ascertain the role of Nollywood film in the transference of Nigerian cultural heritage across other cultures using students at the University of Fort Hare as a case study. This study sought to determine if audience members learn, imbibe or manifest any behavioural tendencies that can be ascribed to the influence of watching Nollywood films. Specifically the study sought to establish the aspect of Nigerian culture that Nollywood films transmit to audience members. The survey research method was used while data was analysed using descriptive statistics. In all, 400 questionnaires were distributed while 370 were returned. Results show that students who were exposed to Nollywood film could mimic some common Nigerian expressions and exclamations. A small percentage of viewers could actually speak a smattering of some Nigerian language while a few have bought or made dresses in the pattern of what they saw in the films. It could be concluded that Nollywood films do exert significant influence on the viewers in the University of Fort Hare.
- Full Text:
- Date Issued: 2015
- «
- ‹
- 1
- ›
- »