Versions of hospitality in recent writing on the fiction of JM Coetzee:
- Authors: Marais, Mike
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144039 , vital:38305 , DOI: 10.4314/eia.v40i1.8
- Description: In one of the interviews in Summertime, Martin, a former colleague, claims that he and the deceased John Coetzee felt their "presence" in South Africa "was legal but illegitimate," that it "was grounded in a crime, namely colonial conquest," which rendered them "sojourners, temporary residents, and to that extent without a home, without a homeland" (209-10). With this statement in mind, Maria J. Lopez argues that a sense of unbelonging underlies J. M. Coetzee's entire oeuvre, including the Australian fiction, and forms a kind of "imaginative and intellectual masterplot" (xii). ). It is this "narrative" that she traces in her monograph, starting with the early fiction and concluding with chapters on the fictionalised autobiographies and Australian fiction.
- Full Text:
- Date Issued: 2013
- Authors: Marais, Mike
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144039 , vital:38305 , DOI: 10.4314/eia.v40i1.8
- Description: In one of the interviews in Summertime, Martin, a former colleague, claims that he and the deceased John Coetzee felt their "presence" in South Africa "was legal but illegitimate," that it "was grounded in a crime, namely colonial conquest," which rendered them "sojourners, temporary residents, and to that extent without a home, without a homeland" (209-10). With this statement in mind, Maria J. Lopez argues that a sense of unbelonging underlies J. M. Coetzee's entire oeuvre, including the Australian fiction, and forms a kind of "imaginative and intellectual masterplot" (xii). ). It is this "narrative" that she traces in her monograph, starting with the early fiction and concluding with chapters on the fictionalised autobiographies and Australian fiction.
- Full Text:
- Date Issued: 2013
Xenophobia, sovereign power and the limits of citizenship:
- Idahosa, Grace E, Vincent, Louise
- Authors: Idahosa, Grace E , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141970 , vital:38020 , DOI: 10.1080/09744053.2014.914637
- Description: African foreigners in South Africa have frequently been the targets of violent and discriminatory practices, which occur in the enabling context of negative discourses concerning African foreigners that circulate in various spheres of public life. This study is interested in one particular field of interaction between African foreigners and the South African state, namely the public health sector. Discriminatory and, sometimes, violent practices towards African foreigners on the part of South African citizens are widely documented. Less discussed are the ways in which these practices of violence and discrimination are in fact state practices. We show this with reference to the treatment of African foreigners in the public health sector. We refer to this prejudicial treatment as health-care Xenophobia which is made possible by a wider set of discourses related to citizenship and the rights accruing to citizens which suggest the ‘non-rights’ of the non-citizen.
- Full Text:
- Date Issued: 2014
- Authors: Idahosa, Grace E , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141970 , vital:38020 , DOI: 10.1080/09744053.2014.914637
- Description: African foreigners in South Africa have frequently been the targets of violent and discriminatory practices, which occur in the enabling context of negative discourses concerning African foreigners that circulate in various spheres of public life. This study is interested in one particular field of interaction between African foreigners and the South African state, namely the public health sector. Discriminatory and, sometimes, violent practices towards African foreigners on the part of South African citizens are widely documented. Less discussed are the ways in which these practices of violence and discrimination are in fact state practices. We show this with reference to the treatment of African foreigners in the public health sector. We refer to this prejudicial treatment as health-care Xenophobia which is made possible by a wider set of discourses related to citizenship and the rights accruing to citizens which suggest the ‘non-rights’ of the non-citizen.
- Full Text:
- Date Issued: 2014
Preliminary assessment of the gender aspects of disaster vulnerability and loss of human life in South Africa
- Tandlich, Roman, Chirenda, Tatenda G, Srinivas, Sunitha C
- Authors: Tandlich, Roman , Chirenda, Tatenda G , Srinivas, Sunitha C
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/76183 , vital:30518 , https://doi.org/10.4102/jamba.v5i2.84
- Description: South Africa has reached a medium level of human development and has a heterogeneous situation with respect to disaster risk management. In this article, a preliminary assessment of the gender aspects of disaster vulnerability and fatalities is presented. The United Nations, the Health Systems Trust and Statistics South Africa were used as data sources for the following gender-segregated values: the life expectancy at birth, unemployment rates, the human development index values, the maternal mortality rates and the number of deaths from unnatural and non-natural causes. The relevant inequality indices were then calculated and used to draw conclusions regarding the gender aspects of disaster risk management in South Africa. Results of the calculations indicate that between 1980 and 2011 men were 10% more vulnerable with respect to their health status. However, the gender differences have been decreasing in recent years. Access of women to healthcare is decreasing with time, potentially decreasing the recovery potential of whole families. Women are more economically vulnerable than men in South Africa, as they are 16.3% – 33% more likely to be unemployed than men. Educational status of both genders in South Africa is comparable based on literacy and enrolment rates at primary and secondary level. On the other hand, men are five times more likely to suffer fatal injuries during disasters.
- Full Text:
- Date Issued: 2013
- Authors: Tandlich, Roman , Chirenda, Tatenda G , Srinivas, Sunitha C
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/76183 , vital:30518 , https://doi.org/10.4102/jamba.v5i2.84
- Description: South Africa has reached a medium level of human development and has a heterogeneous situation with respect to disaster risk management. In this article, a preliminary assessment of the gender aspects of disaster vulnerability and fatalities is presented. The United Nations, the Health Systems Trust and Statistics South Africa were used as data sources for the following gender-segregated values: the life expectancy at birth, unemployment rates, the human development index values, the maternal mortality rates and the number of deaths from unnatural and non-natural causes. The relevant inequality indices were then calculated and used to draw conclusions regarding the gender aspects of disaster risk management in South Africa. Results of the calculations indicate that between 1980 and 2011 men were 10% more vulnerable with respect to their health status. However, the gender differences have been decreasing in recent years. Access of women to healthcare is decreasing with time, potentially decreasing the recovery potential of whole families. Women are more economically vulnerable than men in South Africa, as they are 16.3% – 33% more likely to be unemployed than men. Educational status of both genders in South Africa is comparable based on literacy and enrolment rates at primary and secondary level. On the other hand, men are five times more likely to suffer fatal injuries during disasters.
- Full Text:
- Date Issued: 2013
An Unprecedented 'Precedent'?: Phodiclinics (Pty) Ltd v Pinehaven Private Hospital (Pty) Ltd (594/2010)[2011] ZASCA163 (28 September 2011); 2011 4 All SA 331 (SCA)
- Authors: Glover, Graham B
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69950 , vital:29598 , https://papers.ssrn.com/sol3/papers.cfm?abstract_id=2364288
- Description: There are some fundamental underlying requirements that need to be in place for a system of precedent to operate. Of these I wish to isolate two that are germane to this note. The first is that court decisions (especially those in which a written judgment is handed down) need to be clearly reasoned and logically articulated to be understandable, effective and to have value in a system of precedent that finds its ultimate lodestar in the rule of law. There is no constitutional or statutory requirement that a court in all cases must furnish reasons, or even written reasons, for its decision But the practice of doing so, the Constitutional Court has said, supports the rule of law, ensuring that judicial decision-making does not occur in a manner that is arbitrary, and ensures that judges may be held accountable for their decisions. The second requirement is a technical rule – the rule of majority: if the case is heard by a full bench, and where differing opinions are handed down by the judges hearing the case, the ratio decidendi can only be identified from those judges whose reasoning is in the majority.
- Full Text: false
- Date Issued: 2013
- Authors: Glover, Graham B
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/69950 , vital:29598 , https://papers.ssrn.com/sol3/papers.cfm?abstract_id=2364288
- Description: There are some fundamental underlying requirements that need to be in place for a system of precedent to operate. Of these I wish to isolate two that are germane to this note. The first is that court decisions (especially those in which a written judgment is handed down) need to be clearly reasoned and logically articulated to be understandable, effective and to have value in a system of precedent that finds its ultimate lodestar in the rule of law. There is no constitutional or statutory requirement that a court in all cases must furnish reasons, or even written reasons, for its decision But the practice of doing so, the Constitutional Court has said, supports the rule of law, ensuring that judicial decision-making does not occur in a manner that is arbitrary, and ensures that judges may be held accountable for their decisions. The second requirement is a technical rule – the rule of majority: if the case is heard by a full bench, and where differing opinions are handed down by the judges hearing the case, the ratio decidendi can only be identified from those judges whose reasoning is in the majority.
- Full Text: false
- Date Issued: 2013
Alternative/Experimental art spaces in Johannesburg:
- Authors: Malatjie, Portia
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147692 , vital:38661 , https://0-doi.org.wam.seals.ac.za/10.1080/09528822.2013.798181
- Description: There are a number of recent exciting developments in the Johannesburg alternative art scene. Alternative or experimental art spaces often exist as an alternative to commercial galleries and government or privately funded galleries as well as museums. They are therefore governed through a different set of rules, rules that often aim to transgress the institutional. These spaces, including the Parking Gallery, the Keleketla! Library and arguably the Wits School of Art's Substation gallery, invite debates about alternative art programming, collaborative art making and the necessity of institutional funding in order to maintain such spaces. In addition to these questions, it is pertinent to first determine what it is that classifies a space as alternative.
- Full Text:
- Date Issued: 2013
- Authors: Malatjie, Portia
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147692 , vital:38661 , https://0-doi.org.wam.seals.ac.za/10.1080/09528822.2013.798181
- Description: There are a number of recent exciting developments in the Johannesburg alternative art scene. Alternative or experimental art spaces often exist as an alternative to commercial galleries and government or privately funded galleries as well as museums. They are therefore governed through a different set of rules, rules that often aim to transgress the institutional. These spaces, including the Parking Gallery, the Keleketla! Library and arguably the Wits School of Art's Substation gallery, invite debates about alternative art programming, collaborative art making and the necessity of institutional funding in order to maintain such spaces. In addition to these questions, it is pertinent to first determine what it is that classifies a space as alternative.
- Full Text:
- Date Issued: 2013
Movement patterns and habitat selection of invasive African sharptooth catfish
- Kadye, Wilbert T, Booth, Anthony J
- Authors: Kadye, Wilbert T , Booth, Anthony J
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125276 , vital:35767 , http://dx.doi.10.1111/j.1469-7998.2012.00960.x
- Description: Information on the movement behaviour and habitat use by non-native invasive African catfish Clarias gariepinus is crucial in understanding and possibly mitigating its potential impacts. The aim of this study was to examine catfish movement and habitat selection within an invaded impoundment in the Eastern Cape, South Africa. Acoustic telemetry data for 10 tagged catfish were analyzed to identify spatial patterns in home ranges and seasonal changes in habitat associations. Long-distance movements were observed for most catfish from common central release point, whereas short-distance movements defined their home ranges and utilization distributions that were categorized as localized within single or multiple habitats. Habitat selection was non-random with most catfish utilizing the shallow river mouth and upper section of the reservoir that were dominated by a rocky substratum interspersed with submerged trees. These localities were likely to be preferred for spawning and/or feeding. Utilization of these habitats by catfish is likely to be associated with probable impact due to predation and interference competition for feeding and breeding grounds with other species. Although most catfish maintained their home ranges throughout the study, seasonal shifts in habitat use, which was reflected by the utilization of deep and silt-dominated habitats, were also observed for some catfish. Non-random habitat use and homing behaviour within single and multiple habitats by non-native sharptooth catfish suggests that its impact within the invaded habitats may be associated with particular habitats both at broad spatial and temporal scales. Protection of habitats from catfish invasion should be considered as a management option to conserve native biota.
- Full Text:
- Date Issued: 2013
- Authors: Kadye, Wilbert T , Booth, Anthony J
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125276 , vital:35767 , http://dx.doi.10.1111/j.1469-7998.2012.00960.x
- Description: Information on the movement behaviour and habitat use by non-native invasive African catfish Clarias gariepinus is crucial in understanding and possibly mitigating its potential impacts. The aim of this study was to examine catfish movement and habitat selection within an invaded impoundment in the Eastern Cape, South Africa. Acoustic telemetry data for 10 tagged catfish were analyzed to identify spatial patterns in home ranges and seasonal changes in habitat associations. Long-distance movements were observed for most catfish from common central release point, whereas short-distance movements defined their home ranges and utilization distributions that were categorized as localized within single or multiple habitats. Habitat selection was non-random with most catfish utilizing the shallow river mouth and upper section of the reservoir that were dominated by a rocky substratum interspersed with submerged trees. These localities were likely to be preferred for spawning and/or feeding. Utilization of these habitats by catfish is likely to be associated with probable impact due to predation and interference competition for feeding and breeding grounds with other species. Although most catfish maintained their home ranges throughout the study, seasonal shifts in habitat use, which was reflected by the utilization of deep and silt-dominated habitats, were also observed for some catfish. Non-random habitat use and homing behaviour within single and multiple habitats by non-native sharptooth catfish suggests that its impact within the invaded habitats may be associated with particular habitats both at broad spatial and temporal scales. Protection of habitats from catfish invasion should be considered as a management option to conserve native biota.
- Full Text:
- Date Issued: 2013
'The Area of a Triangle is 1800C’: an analysis of Learners' Idiosyncratic Geometry Responses through the Lenses of Vygotsky's Theory of Concept Formation
- Mhlolo, Michael K, Schäfer, Marc
- Authors: Mhlolo, Michael K , Schäfer, Marc
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141127 , vital:37946 , DOI: 10.1080/10288457.2013.826973
- Description: In this paper, we focus specifically on Vygotsky's theory of concept formation to gain initial insights into seemingly garbled and incoherent connections we observed in learners' responses to a geometry task. While acknowledging research that is supportive of the van Hiele model as being useful when analysing concept formation in geometry, in this paper we use empirical evidence to argue that today's geometry requires learners to reason with many tools which the van Hiele model does not seem to accommodate. Working with 470 written responses of 12–14-year-old learners to a geometry task, we then explored the potential of Vygotsky's theory of concept formation as an alternative framework. We conclude by discussing the paper's contribution both to theory and to classroom practice.
- Full Text:
- Date Issued: 2013
- Authors: Mhlolo, Michael K , Schäfer, Marc
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141127 , vital:37946 , DOI: 10.1080/10288457.2013.826973
- Description: In this paper, we focus specifically on Vygotsky's theory of concept formation to gain initial insights into seemingly garbled and incoherent connections we observed in learners' responses to a geometry task. While acknowledging research that is supportive of the van Hiele model as being useful when analysing concept formation in geometry, in this paper we use empirical evidence to argue that today's geometry requires learners to reason with many tools which the van Hiele model does not seem to accommodate. Working with 470 written responses of 12–14-year-old learners to a geometry task, we then explored the potential of Vygotsky's theory of concept formation as an alternative framework. We conclude by discussing the paper's contribution both to theory and to classroom practice.
- Full Text:
- Date Issued: 2013
ICT as an enabler of Socio Economic Development
- Authors: Van Greunen, Darrelle
- Subjects: Information technology , Digital divide , f-sa
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/10948/20725 , vital:29383
- Description: Today’s is a world of many divides, one of the most typical being the Digital Divide, which in itself has given birth to or is worsening other economic and social divides. In this world, more suffer and less are able to benefit from technology. This paper aims to promote the importance of and need for inter-disciplinary cooperation for the use and promotion of Information and Communication Technologies (ICT) as a bridge for the Digital Divide within disciplines. Information and communication technologies (ICTs) include any communication device—radio, television, cellular phones, computer and network hardware and software, satellite systems, the services and applications associated with them, such as the Internet, geographic positioning systems (GPS), banking, e-health, e-learning and electronic government services. The paper will touch on tangible examples of inter-disciplinary cooperation and the use of ICT in different community interventions.
- Full Text:
- Authors: Van Greunen, Darrelle
- Subjects: Information technology , Digital divide , f-sa
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/10948/20725 , vital:29383
- Description: Today’s is a world of many divides, one of the most typical being the Digital Divide, which in itself has given birth to or is worsening other economic and social divides. In this world, more suffer and less are able to benefit from technology. This paper aims to promote the importance of and need for inter-disciplinary cooperation for the use and promotion of Information and Communication Technologies (ICT) as a bridge for the Digital Divide within disciplines. Information and communication technologies (ICTs) include any communication device—radio, television, cellular phones, computer and network hardware and software, satellite systems, the services and applications associated with them, such as the Internet, geographic positioning systems (GPS), banking, e-health, e-learning and electronic government services. The paper will touch on tangible examples of inter-disciplinary cooperation and the use of ICT in different community interventions.
- Full Text:
“Face the music!”: the Daily Sun's representation of adolescent sex in the Jules High sex scandal
- Boshoff, Priscilla A, Prinsloo, Jeanne
- Authors: Boshoff, Priscilla A , Prinsloo, Jeanne
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143483 , vital:38250 , DOI: 10.1080/10130950.2013.834660
- Description: Rather than being merely a physiological stage, adolescence is variously constructed through social institutions. The media plays a significant role in such constructions, including that of adolescent sexuality. In the recent past there have been several cases of sexual acts involving adolescents that have received prominent media coverage as they have been considered shocking. The Jules High School sex scandal related to sex acts between a single adolescent girl and two adolescent boys. It was recorded on mobile phones by their peers and circulated on their mobile networks, or ‘went viral’ as the media continuously noted. The press coverage surrounding this incident and the legal process that ensued is the focus of this Article which undertakes a critical textual analysis of the coverage in the popular tabloid, the Daily Sun, in order to make explicit the contesting sets of discourses around adolescence and sexuality that were articulated in this popular public sphere. The Article uses a Foucauldian framework in order to probe the discourses of sexuality that are articulated and contested in this space. As the most widely read newspaper in South Africa it serves as a powerful site of definition of teen sexuality. The analysis suggests that, rather than allowing for teen sexuality, it is disavowed by villainising teen sex and responsibility for such ‘deviance’ is directed to various adult and social adult actors.
- Full Text:
- Date Issued: 2013
- Authors: Boshoff, Priscilla A , Prinsloo, Jeanne
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143483 , vital:38250 , DOI: 10.1080/10130950.2013.834660
- Description: Rather than being merely a physiological stage, adolescence is variously constructed through social institutions. The media plays a significant role in such constructions, including that of adolescent sexuality. In the recent past there have been several cases of sexual acts involving adolescents that have received prominent media coverage as they have been considered shocking. The Jules High School sex scandal related to sex acts between a single adolescent girl and two adolescent boys. It was recorded on mobile phones by their peers and circulated on their mobile networks, or ‘went viral’ as the media continuously noted. The press coverage surrounding this incident and the legal process that ensued is the focus of this Article which undertakes a critical textual analysis of the coverage in the popular tabloid, the Daily Sun, in order to make explicit the contesting sets of discourses around adolescence and sexuality that were articulated in this popular public sphere. The Article uses a Foucauldian framework in order to probe the discourses of sexuality that are articulated and contested in this space. As the most widely read newspaper in South Africa it serves as a powerful site of definition of teen sexuality. The analysis suggests that, rather than allowing for teen sexuality, it is disavowed by villainising teen sex and responsibility for such ‘deviance’ is directed to various adult and social adult actors.
- Full Text:
- Date Issued: 2013
Assessment of the long-term response to rehabilitation of two wetlands in KwaZulu-Natal, South Africa:
- Cowden, Craig, Kotze, Donovan C, Ellery, William F N, Sieben, E J J
- Authors: Cowden, Craig , Kotze, Donovan C , Ellery, William F N , Sieben, E J J
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144431 , vital:38345 , DOI: 10.2989/16085914.2014.954518
- Description: Assessing the ecological outcomes of wetland rehabilitation activities is an important need recognised by the ‘Working for Wetlands’ programme in South Africa. An assessment of ecological response was conducted in the Killarney and Kruisfontein wetlands, KwaZulu-Natal, in 2005 prior to rehabilitation in 2006, and again in 2011 and 2012, respectively, following rehabilitation. The assessment criteria included an evaluation of changes in ecological integrity, the supply of ecosystem services, and vegetation composition. Improvements in hydrological and geomorphic integrity were recorded in both wetlands, resulting in improved ecosystem delivery.
- Full Text:
- Date Issued: 2014
- Authors: Cowden, Craig , Kotze, Donovan C , Ellery, William F N , Sieben, E J J
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144431 , vital:38345 , DOI: 10.2989/16085914.2014.954518
- Description: Assessing the ecological outcomes of wetland rehabilitation activities is an important need recognised by the ‘Working for Wetlands’ programme in South Africa. An assessment of ecological response was conducted in the Killarney and Kruisfontein wetlands, KwaZulu-Natal, in 2005 prior to rehabilitation in 2006, and again in 2011 and 2012, respectively, following rehabilitation. The assessment criteria included an evaluation of changes in ecological integrity, the supply of ecosystem services, and vegetation composition. Improvements in hydrological and geomorphic integrity were recorded in both wetlands, resulting in improved ecosystem delivery.
- Full Text:
- Date Issued: 2014
13C pulse-chase labeling comparative assessment of the active methanogenic archaeal community composition in the transgenic and nontransgenic parental rice rhizospheres
- Zhu, Weijing, Lu, Haohao, Hill, Jaclyn M, Wang, Hailong, Wu, Weixiang
- Authors: Zhu, Weijing , Lu, Haohao , Hill, Jaclyn M , Wang, Hailong , Wu, Weixiang
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/122993 , vital:35389 , https://doi.org/10.1111/1574-6941.12261
- Description: Rhabdosargus holubi (Steindachner, 1881) is a small (maximum size = 450 mm total length; Heemstra and Heemstra 2004) sparid that is distributed along the south-east coast of Africa from St Helena Bay, South Africa, to Maputo, Mozambique (Götz and Cowley 2013). Spawning occurs in the nearshore marine environment primarily during winter, specifically May–August in KwaZulu-Natal (KZN) (Wallace 1975) and July–February in the South-Eastern Cape (Whitfield 1998). Individuals reach 50% sexual maturity at approximately 150 mm standard length (SL) in the Eastern Cape (Whitfield 1998). The early life stages are transported by the south-westward-flowing Agulhas Current, and recruit as post-flexion larvae and early juveniles into estuaries during late winter and early summer (Blaber 1974). The warm temperatures and high nutrient levels in estuaries favour fast growth (Blaber 1973a), and fish spend their first year of life in these environments, migrating back out to sea after reaching approximately 120 mm SL. Some individuals remain trapped in closed estuaries, where they may reach sizes greater than 200 mm SL (James et al. 2007a). Rhabdosargus holubi is the dominant estuarine-dependent marine teleost species recorded in permanently open and temporarily open/closed estuaries in the warm-temperate region, which spans the south, south-east and east coast of South Africa (Harrison 2005). The species is also an important component of the linefishery in many SouthAfrican estuaries (10–15.6% by number) (Pradervand and Baird 2002), particularly in Eastern Cape estuaries (Cowley et al. 2003). These figures underestimate the presence of R. holubi, as most individuals making use of estuaries are young, feeding predominately on filamentous macroalgae and diatom flora, and are generally too small to be caught with hook and line (De Wet and Marais 1990). James et al. (2007b) showed that R. holubi made up 34–92% of the annual seine-net catch in the East Kleinemonde Estuary. Rhabdosargus holubi is also important in the KZN shorebased linefishery, representing 4.6% of the total landed catch (Dunlop and Mann 2012)More and more investigations indicate that genetic modification has no significant or persistent effects on microbial community composition in the rice rhizosphere. Very few studies, however, have focused on its impact on functional microorganisms. This study completed a 13C-CO2 pulse-chase labeling experiment comparing the potential effects of cry1Ab gene transformation on 13C tissue distribution and rhizosphere methanogenic archaeal community composition with its parental rice variety (Ck) and a distant parental rice variety (Dp). Results showed that 13C partitioning in aboveground biomass (mainly in stems) and roots of Dp was significantly lower than that of Ck. However, there were no significant differences in 13C partitioning between the Bt transgenic rice line (Bt) and Ck. RNA-stable isotope probing combined with clone library analyses inferred that the group Methanosaetaceae was the predominant methanogenic Archaea in all three rice rhizospheres. The active methanogenic archaeal community in the Bt rhizosphere was dominated by Methanosarcinaceae, Methanosaetaceae, and Methanomicrobiaceae, while there were only two main methanogenic clusters (Methanosaetaceae and Methanomicrobiaceae) in the Ck and Dp rhizospheres. These results indicate that the insertion of cry1Ab gene into the rice genome has the potential to result in the modification of methanogenic community composition in its rhizosphere.
- Full Text:
- Date Issued: 2013
- Authors: Zhu, Weijing , Lu, Haohao , Hill, Jaclyn M , Wang, Hailong , Wu, Weixiang
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/122993 , vital:35389 , https://doi.org/10.1111/1574-6941.12261
- Description: Rhabdosargus holubi (Steindachner, 1881) is a small (maximum size = 450 mm total length; Heemstra and Heemstra 2004) sparid that is distributed along the south-east coast of Africa from St Helena Bay, South Africa, to Maputo, Mozambique (Götz and Cowley 2013). Spawning occurs in the nearshore marine environment primarily during winter, specifically May–August in KwaZulu-Natal (KZN) (Wallace 1975) and July–February in the South-Eastern Cape (Whitfield 1998). Individuals reach 50% sexual maturity at approximately 150 mm standard length (SL) in the Eastern Cape (Whitfield 1998). The early life stages are transported by the south-westward-flowing Agulhas Current, and recruit as post-flexion larvae and early juveniles into estuaries during late winter and early summer (Blaber 1974). The warm temperatures and high nutrient levels in estuaries favour fast growth (Blaber 1973a), and fish spend their first year of life in these environments, migrating back out to sea after reaching approximately 120 mm SL. Some individuals remain trapped in closed estuaries, where they may reach sizes greater than 200 mm SL (James et al. 2007a). Rhabdosargus holubi is the dominant estuarine-dependent marine teleost species recorded in permanently open and temporarily open/closed estuaries in the warm-temperate region, which spans the south, south-east and east coast of South Africa (Harrison 2005). The species is also an important component of the linefishery in many SouthAfrican estuaries (10–15.6% by number) (Pradervand and Baird 2002), particularly in Eastern Cape estuaries (Cowley et al. 2003). These figures underestimate the presence of R. holubi, as most individuals making use of estuaries are young, feeding predominately on filamentous macroalgae and diatom flora, and are generally too small to be caught with hook and line (De Wet and Marais 1990). James et al. (2007b) showed that R. holubi made up 34–92% of the annual seine-net catch in the East Kleinemonde Estuary. Rhabdosargus holubi is also important in the KZN shorebased linefishery, representing 4.6% of the total landed catch (Dunlop and Mann 2012)More and more investigations indicate that genetic modification has no significant or persistent effects on microbial community composition in the rice rhizosphere. Very few studies, however, have focused on its impact on functional microorganisms. This study completed a 13C-CO2 pulse-chase labeling experiment comparing the potential effects of cry1Ab gene transformation on 13C tissue distribution and rhizosphere methanogenic archaeal community composition with its parental rice variety (Ck) and a distant parental rice variety (Dp). Results showed that 13C partitioning in aboveground biomass (mainly in stems) and roots of Dp was significantly lower than that of Ck. However, there were no significant differences in 13C partitioning between the Bt transgenic rice line (Bt) and Ck. RNA-stable isotope probing combined with clone library analyses inferred that the group Methanosaetaceae was the predominant methanogenic Archaea in all three rice rhizospheres. The active methanogenic archaeal community in the Bt rhizosphere was dominated by Methanosarcinaceae, Methanosaetaceae, and Methanomicrobiaceae, while there were only two main methanogenic clusters (Methanosaetaceae and Methanomicrobiaceae) in the Ck and Dp rhizospheres. These results indicate that the insertion of cry1Ab gene into the rice genome has the potential to result in the modification of methanogenic community composition in its rhizosphere.
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- Date Issued: 2013
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