Reduction in transport in wheat (Triticum aestivum) is caused by sustained phloem feeding by the Russian wheat aphid (Diuraphis noxia)
- Botha, Christiaan E J, Matsiliza, Babalwa
- Authors: Botha, Christiaan E J , Matsiliza, Babalwa
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6498 , http://hdl.handle.net/10962/d1004498
- Description: The Russian wheat aphid (Diuraphis noxia) feeds preferentially from the phloem of longitudinal veins of nonresistant wheat leaves. The xenobiotic, 5,6-CFDA was applied to exposed leaf blade mesophyll cells in control and aphid-infested plants. In control plants, the fluorophore moved approximately 5–6cm from the point of application of 5,6-CFDA within 3h of application. The fluorochrome was transported in the sieve tube companion cell complex, including those in the numerous interconnecting transverse veins. Leaf blades on which the Russian wheat aphid had been feeding demonstrated a marked decrease in 5,6-CF transport. Aphids feeding on the leaves formed local sinks and redirected the fluorophore (and presumably associated assimilate) to the aphids’ gut, with little longitudinal translocation of 5,6-CF below inserted stylets or aphid feeding areas. Aniline blue staining revealed massive deposits of wound and reaction callose caused by the aphids, with callose associated with the sieve plates, pore-plasmodesma between the companion cells and their associated sieve tubes, as well as with plasmodesmal aggregates in parenchymatous elements within the vascular bundles. Leaves that had been colonised by aphids but from which the aphids were removed, showed extensive wound callose deposits, which persisted for up to 48h after removal of aphid colonies, suggesting that the damage caused by aphid feeding is a long-term, non-transient event in non-resistant plants.
- Full Text:
- Date Issued: 2004
- Authors: Botha, Christiaan E J , Matsiliza, Babalwa
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6498 , http://hdl.handle.net/10962/d1004498
- Description: The Russian wheat aphid (Diuraphis noxia) feeds preferentially from the phloem of longitudinal veins of nonresistant wheat leaves. The xenobiotic, 5,6-CFDA was applied to exposed leaf blade mesophyll cells in control and aphid-infested plants. In control plants, the fluorophore moved approximately 5–6cm from the point of application of 5,6-CFDA within 3h of application. The fluorochrome was transported in the sieve tube companion cell complex, including those in the numerous interconnecting transverse veins. Leaf blades on which the Russian wheat aphid had been feeding demonstrated a marked decrease in 5,6-CF transport. Aphids feeding on the leaves formed local sinks and redirected the fluorophore (and presumably associated assimilate) to the aphids’ gut, with little longitudinal translocation of 5,6-CF below inserted stylets or aphid feeding areas. Aniline blue staining revealed massive deposits of wound and reaction callose caused by the aphids, with callose associated with the sieve plates, pore-plasmodesma between the companion cells and their associated sieve tubes, as well as with plasmodesmal aggregates in parenchymatous elements within the vascular bundles. Leaves that had been colonised by aphids but from which the aphids were removed, showed extensive wound callose deposits, which persisted for up to 48h after removal of aphid colonies, suggesting that the damage caused by aphid feeding is a long-term, non-transient event in non-resistant plants.
- Full Text:
- Date Issued: 2004
Pictograms for conveying medicine instructions: comprehension in various South African language groups
- Dowse, Roslind, Ehlers, Martina S
- Authors: Dowse, Roslind , Ehlers, Martina S
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6354 , http://hdl.handle.net/10962/d1006038
- Description: The comprehension of medicine instructions is essential for the safe and effective use of medicines. In cases where low literacy constitutes a possible barrier to understanding written medicine information fully, the inclusion of pictograms may facilitate comprehension. Twenty-three internationally available pictograms and 23 corresponding locally developed images were evaluated in 304 low-literate respondents from eight different South African language groups. Demographic data were collected and an English literacy test was administered to those respondents who had stated that they could read English. Interviewees were shown the 46 pictograms in random order and were asked for their interpretation. Preference for either the international or local version was assessed. Correct interpretation of individual pictograms ranged from 14% to 97%. Images which had been developed locally were more successful in eliciting correct interpretations than those obtained from an international source (USP pictograms). Only 2 of the 23 USP pictograms achieved the 85% criterion of the American National Standards Institute compared with 12 of 23 locally developed counterparts. Local pictograms were preferred over the American ones in all cases. Standard of education had a significant influence on interpretation, whereas no significant differences in interpretation could be attributed to cultural diversity between the African language groups. Almost all respondents (98%) reacted positively to the idea of having pictograms on their medicine labels. The success of the local pictograms highlighted the value of a rigorous and consultative design and test process. Results from one African language group can reliably be extrapolated to other groups in South Africa.
- Full Text:
- Date Issued: 2004
- Authors: Dowse, Roslind , Ehlers, Martina S
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6354 , http://hdl.handle.net/10962/d1006038
- Description: The comprehension of medicine instructions is essential for the safe and effective use of medicines. In cases where low literacy constitutes a possible barrier to understanding written medicine information fully, the inclusion of pictograms may facilitate comprehension. Twenty-three internationally available pictograms and 23 corresponding locally developed images were evaluated in 304 low-literate respondents from eight different South African language groups. Demographic data were collected and an English literacy test was administered to those respondents who had stated that they could read English. Interviewees were shown the 46 pictograms in random order and were asked for their interpretation. Preference for either the international or local version was assessed. Correct interpretation of individual pictograms ranged from 14% to 97%. Images which had been developed locally were more successful in eliciting correct interpretations than those obtained from an international source (USP pictograms). Only 2 of the 23 USP pictograms achieved the 85% criterion of the American National Standards Institute compared with 12 of 23 locally developed counterparts. Local pictograms were preferred over the American ones in all cases. Standard of education had a significant influence on interpretation, whereas no significant differences in interpretation could be attributed to cultural diversity between the African language groups. Almost all respondents (98%) reacted positively to the idea of having pictograms on their medicine labels. The success of the local pictograms highlighted the value of a rigorous and consultative design and test process. Results from one African language group can reliably be extrapolated to other groups in South Africa.
- Full Text:
- Date Issued: 2004
In situ feeding rates of the copepods, Pseudodiaptomus hessei and Acartia longipatella, in a temperate, temporarily open/closed Eastern Cape estuary
- Authors: Froneman, P William
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6901 , http://hdl.handle.net/10962/d1011754
- Description: Size-fractionated chlorophyll-a (chl-a) concentrations and the in situ grazing rates of the copepods, Pseudodiaptomus hessei and Acartia longipatella, were assessed seasonally at the temporarily open/closed Kasouga estuary situated along the southeast coast of southern Africa. Total integrated chl-a concentration ranged between 1.17 and 12.18 mg chl-a m^(–3) and was always dominated by small phytoplankton cells (<20 μm), which comprised up to 86% (range 64–86%) of the total pigment. Total zooplankton abundance ranged between 2676 and 62 043 individuals m^(–3). These copepods numerically dominated the zooplankton counts, accounting for between 79% and 91% of the total. Gut pigment concentrations of the two species at night were significantly higher than the daytime values (P<0.05 in all cases). The observed pattern could be related to the marked diurnal vertical migration patterns exhibited by the copepods. Gut evacuation rates of P. hessei during the study ranged between 0.29 and 0.77 h^(–1) and between 0.39 and 0.58 h^(–1) for A. longipatella. The rate of gut pigment destruction for P. hessei and A. longipatella ranged between 55% and 81% and between 88% and 92% of the total chl-a ingested, respectively. The combined grazing impact of the two copepods ranged between 0.65 and 4.37 mg chl-a m^(–3), or between 4.3% and 35.9% of the available chl-a in the water column. Variations in the grazing activity of the two species could be attributed largely to seasonality in water temperature and shifts in the phytoplankton community structure and zooplankton abundance.
- Full Text:
- Date Issued: 2004
- Authors: Froneman, P William
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6901 , http://hdl.handle.net/10962/d1011754
- Description: Size-fractionated chlorophyll-a (chl-a) concentrations and the in situ grazing rates of the copepods, Pseudodiaptomus hessei and Acartia longipatella, were assessed seasonally at the temporarily open/closed Kasouga estuary situated along the southeast coast of southern Africa. Total integrated chl-a concentration ranged between 1.17 and 12.18 mg chl-a m^(–3) and was always dominated by small phytoplankton cells (<20 μm), which comprised up to 86% (range 64–86%) of the total pigment. Total zooplankton abundance ranged between 2676 and 62 043 individuals m^(–3). These copepods numerically dominated the zooplankton counts, accounting for between 79% and 91% of the total. Gut pigment concentrations of the two species at night were significantly higher than the daytime values (P<0.05 in all cases). The observed pattern could be related to the marked diurnal vertical migration patterns exhibited by the copepods. Gut evacuation rates of P. hessei during the study ranged between 0.29 and 0.77 h^(–1) and between 0.39 and 0.58 h^(–1) for A. longipatella. The rate of gut pigment destruction for P. hessei and A. longipatella ranged between 55% and 81% and between 88% and 92% of the total chl-a ingested, respectively. The combined grazing impact of the two copepods ranged between 0.65 and 4.37 mg chl-a m^(–3), or between 4.3% and 35.9% of the available chl-a in the water column. Variations in the grazing activity of the two species could be attributed largely to seasonality in water temperature and shifts in the phytoplankton community structure and zooplankton abundance.
- Full Text:
- Date Issued: 2004
Rhizome connections...
- Authors: Lotz-Sisitka, Heila
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6097 , http://hdl.handle.net/10962/d1008620
- Description: There are a number of papers in the journal that reflect a trend in environmental education towards deliberating recuperative, relational epistemologies. In their paper addressing the near-schism between those that appear to be antagonistic to post-structuralism and deconstruction, and those that find them generative in their inquiries, Noel Gough and Leigh Price go right to the heart of human inquiry by questioning the most commonly held assumption in the research enterprise – that the social sciences require a different methodology from the natural sciences.Through giving attention to relativist (constructionist) epistemology and a stratified, realist ontology – which assumes a relational account of ontology – they suggest the same basic methodology for both the social and natural sciences, arguing that ‘... society and humans mutually transform/reproduce each other, just as nature and humans mutually transform/reproduce each other’. In doing this, they address over-simplified dialectics between ‘constructionism’ and realism which has shaped much human inquiry (including environmental education research).
- Full Text:
- Date Issued: 2004
- Authors: Lotz-Sisitka, Heila
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6097 , http://hdl.handle.net/10962/d1008620
- Description: There are a number of papers in the journal that reflect a trend in environmental education towards deliberating recuperative, relational epistemologies. In their paper addressing the near-schism between those that appear to be antagonistic to post-structuralism and deconstruction, and those that find them generative in their inquiries, Noel Gough and Leigh Price go right to the heart of human inquiry by questioning the most commonly held assumption in the research enterprise – that the social sciences require a different methodology from the natural sciences.Through giving attention to relativist (constructionist) epistemology and a stratified, realist ontology – which assumes a relational account of ontology – they suggest the same basic methodology for both the social and natural sciences, arguing that ‘... society and humans mutually transform/reproduce each other, just as nature and humans mutually transform/reproduce each other’. In doing this, they address over-simplified dialectics between ‘constructionism’ and realism which has shaped much human inquiry (including environmental education research).
- Full Text:
- Date Issued: 2004
The Southern Ocean Group at Rhodes University: seventeen years of biological oceanography in the Southern Ocean reviewed
- McQuaid, Christopher D, Froneman, P William
- Authors: McQuaid, Christopher D , Froneman, P William
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6834 , http://hdl.handle.net/10962/d1010423
- Description: This paper reviews the main findings of the Southern Ocean Group at Rhodes University over the last 17 years. A primary contribution has been the development of conceptual models of the physical-biological driving mechanisms that support enormous seasonal populations of land-based top predators at the Prince Edward Islands. Collectively, these models are referred to as the life-support system of the islands. Near-shore subcomponents of the ecosystem, including inshore feeding predators, are largely supported by autochthonous primary production of kelps and localized diatom blooms. These energy sources feed indirectly into top predator populations via the benthic communities. A crucial link is formed by the bottom-dwelling shrimp, Nauticaris marionis, which feeds largely on benthic species and detritus and is eaten by a number of diving seabirds. The frontal systems that lie north and south of the islands are important feeding grounds for offshore feeding birds. A decadal-scale southward shift in the position of the Sub-antarctic Front towards the islands is reflected in increases in populations of these species. , Rhodes Centenary issue
- Full Text:
- Date Issued: 2004
- Authors: McQuaid, Christopher D , Froneman, P William
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6834 , http://hdl.handle.net/10962/d1010423
- Description: This paper reviews the main findings of the Southern Ocean Group at Rhodes University over the last 17 years. A primary contribution has been the development of conceptual models of the physical-biological driving mechanisms that support enormous seasonal populations of land-based top predators at the Prince Edward Islands. Collectively, these models are referred to as the life-support system of the islands. Near-shore subcomponents of the ecosystem, including inshore feeding predators, are largely supported by autochthonous primary production of kelps and localized diatom blooms. These energy sources feed indirectly into top predator populations via the benthic communities. A crucial link is formed by the bottom-dwelling shrimp, Nauticaris marionis, which feeds largely on benthic species and detritus and is eaten by a number of diving seabirds. The frontal systems that lie north and south of the islands are important feeding grounds for offshore feeding birds. A decadal-scale southward shift in the position of the Sub-antarctic Front towards the islands is reflected in increases in populations of these species. , Rhodes Centenary issue
- Full Text:
- Date Issued: 2004
Evidence for Archaean lamprophyre from the Kaapvaal Craton, South Africa
- Prevec, S A, Anhaeusser, C R, Poujol, M
- Authors: Prevec, S A , Anhaeusser, C R , Poujol, M
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6739 , http://hdl.handle.net/10962/d1007555
- Description: A suite of mafic dykes occurs as a late component in a wellcharacterized trondhjemite–tonalite–diorite–granodiorite assemblage in the Johannesburg Dome of the central Kaapvaal Craton, southern Africa. The dykes have been subdivided into two sets, based on their orientation, and major and trace element geochemistry. Set 1 dykes are characterized by elevated SiO2, Al2O3 and TiO2, and particularly by enriched LILE and HSFE (e.g. Zr > 200 ppm, Nb > 20 ppm, Ba > 300 ppm), higher than in any of the accompanying felsic rocks. REE and trace element values for Set 1 dykes are similar to those for calc-alkaline lamprophyres. The Set 2 dykes have similar trace element distributions, but are significantly less enriched in general, and are broadly tholeiitic in composition, with enriched MgO (>11 wt. percentage) indicative of an olivine–phyric tholeiitic basaltic protolith. Field relationships and available U–Pb zircon geochronology indicate that the dykes are contemporaneous with components of the trondhjemitic host rocks, and with late granodiorites. The geochemical, geochronological and field petrological setting indicates partial melting of basaltic and eclogitic lithosphere at c. 3120 Myr ago in the basal Kaapvaal Craton, and subsequent emplacement into pre-existing c. 3430 Myr tonalitic to dioritic crust.
- Full Text:
- Date Issued: 2004
- Authors: Prevec, S A , Anhaeusser, C R , Poujol, M
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6739 , http://hdl.handle.net/10962/d1007555
- Description: A suite of mafic dykes occurs as a late component in a wellcharacterized trondhjemite–tonalite–diorite–granodiorite assemblage in the Johannesburg Dome of the central Kaapvaal Craton, southern Africa. The dykes have been subdivided into two sets, based on their orientation, and major and trace element geochemistry. Set 1 dykes are characterized by elevated SiO2, Al2O3 and TiO2, and particularly by enriched LILE and HSFE (e.g. Zr > 200 ppm, Nb > 20 ppm, Ba > 300 ppm), higher than in any of the accompanying felsic rocks. REE and trace element values for Set 1 dykes are similar to those for calc-alkaline lamprophyres. The Set 2 dykes have similar trace element distributions, but are significantly less enriched in general, and are broadly tholeiitic in composition, with enriched MgO (>11 wt. percentage) indicative of an olivine–phyric tholeiitic basaltic protolith. Field relationships and available U–Pb zircon geochronology indicate that the dykes are contemporaneous with components of the trondhjemitic host rocks, and with late granodiorites. The geochemical, geochronological and field petrological setting indicates partial melting of basaltic and eclogitic lithosphere at c. 3120 Myr ago in the basal Kaapvaal Craton, and subsequent emplacement into pre-existing c. 3430 Myr tonalitic to dioritic crust.
- Full Text:
- Date Issued: 2004
The importance of non-timber forest products in rural livelihood security and as safety nets: a review of evidence from South Africa
- Shackleton, Charlie M, Shackleton, Sheona E
- Authors: Shackleton, Charlie M , Shackleton, Sheona E
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6650 , http://hdl.handle.net/10962/d1007061
- Description: We review and synthesize recent South African work that examines the role and importance of non-timber forest products (NTFPs) in the daily lives of rural people in South Africa. The most commonly used such products are wild spinaches, fuelwood, wooden utensils edible fruits, grass hand-brushes, and twig hand-brushes, used by 85% or more of households. More than half the households investigated also make use of edible insects, wood for construction, bushmeat, wild honey and reeds for weaving. Individual households may exploit dozens of animal and plant species. The range in annual, direct-use values is large, from less than R1000 per household per year to over R12 000. The value to rural households is manifest through a daily net function which represents a cost saving to the families involved and to the state, as well as through an emergency net, which serves as an insurance in times of misfortune, such as drought, disease, and unexpected economic hardship. The emergency net function has hardly been quantified in South Africa and internationally. Ad hoc trade in NTFPs is a common emergency net, which in some instances evolves into a permanent way of life. Financial returns from trade are variable, depending on resource type and hours worked, but are typically low. Despite the small cash incomes from trade, they provide an important contribution that complement the diverse livelihood strategies within a household, especially for the poorer sectors of rural society. Moreover, there are non-financial benefits of NTFP trade that are commonly overlooked.
- Full Text:
- Date Issued: 2004
- Authors: Shackleton, Charlie M , Shackleton, Sheona E
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6650 , http://hdl.handle.net/10962/d1007061
- Description: We review and synthesize recent South African work that examines the role and importance of non-timber forest products (NTFPs) in the daily lives of rural people in South Africa. The most commonly used such products are wild spinaches, fuelwood, wooden utensils edible fruits, grass hand-brushes, and twig hand-brushes, used by 85% or more of households. More than half the households investigated also make use of edible insects, wood for construction, bushmeat, wild honey and reeds for weaving. Individual households may exploit dozens of animal and plant species. The range in annual, direct-use values is large, from less than R1000 per household per year to over R12 000. The value to rural households is manifest through a daily net function which represents a cost saving to the families involved and to the state, as well as through an emergency net, which serves as an insurance in times of misfortune, such as drought, disease, and unexpected economic hardship. The emergency net function has hardly been quantified in South Africa and internationally. Ad hoc trade in NTFPs is a common emergency net, which in some instances evolves into a permanent way of life. Financial returns from trade are variable, depending on resource type and hours worked, but are typically low. Despite the small cash incomes from trade, they provide an important contribution that complement the diverse livelihood strategies within a household, especially for the poorer sectors of rural society. Moreover, there are non-financial benefits of NTFP trade that are commonly overlooked.
- Full Text:
- Date Issued: 2004
Livelihood benefits from the local level commercialization of savanna resources: a case study of the new and expanding trade in marula (Sclerocarya birrea) beer in Bushbuckridge, South Africa
- Authors: Shackleton, Sheona E
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6649 , http://hdl.handle.net/10962/d1007060
- Description: In the face of economic hardship and poverty, rural people in South Africa and globally are increasingly turning to the natural resource base to generate income. One product that has recently entered into local commercial markets is a traditional alcoholic beverage brewed from the fruits of Sclerocarya birrea (marula), commonly known as marula beer. It was rarely traded in the past due to customary taboos prohibiting its sale. In Bushbuckridge, Limpopo province, rural women have been selling marula beer in the business centres of the district and along the roadside since 1998. A survey of these markets was undertaken in 2002, to discover the commercialization process involved, from raw material harvesting to marketing, and to assess the importance of the trade for household livelihoods and poverty alleviation. Fifty-one traders were interviewed, who indicated that the sale of marula beer provides a vital source of income to several hundred households from amongst the poorest sector of the Bushbuckridge community. Of particular importance were the low barriers of entry to the trade, as the resource is abundant and freely accessible and there are few costs to produce marula beer. Incomes earned were modest, averaging a net R500 per season, and highly seasonal, but came at a critical point in the household calendar, after the festive season and when cash was required for the new school year. Income bridging, livelihood diversification, and the provision of a safety net for the poorest members of society are some of the key benefits of the beer trade.
- Full Text:
- Date Issued: 2004
- Authors: Shackleton, Sheona E
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6649 , http://hdl.handle.net/10962/d1007060
- Description: In the face of economic hardship and poverty, rural people in South Africa and globally are increasingly turning to the natural resource base to generate income. One product that has recently entered into local commercial markets is a traditional alcoholic beverage brewed from the fruits of Sclerocarya birrea (marula), commonly known as marula beer. It was rarely traded in the past due to customary taboos prohibiting its sale. In Bushbuckridge, Limpopo province, rural women have been selling marula beer in the business centres of the district and along the roadside since 1998. A survey of these markets was undertaken in 2002, to discover the commercialization process involved, from raw material harvesting to marketing, and to assess the importance of the trade for household livelihoods and poverty alleviation. Fifty-one traders were interviewed, who indicated that the sale of marula beer provides a vital source of income to several hundred households from amongst the poorest sector of the Bushbuckridge community. Of particular importance were the low barriers of entry to the trade, as the resource is abundant and freely accessible and there are few costs to produce marula beer. Incomes earned were modest, averaging a net R500 per season, and highly seasonal, but came at a critical point in the household calendar, after the festive season and when cash was required for the new school year. Income bridging, livelihood diversification, and the provision of a safety net for the poorest members of society are some of the key benefits of the beer trade.
- Full Text:
- Date Issued: 2004
Recent African derivation of Chrysomya putoria from C. chloropyga and mitochondrial DNA paraphyly of cytochrome oxidase subunit one in blowflies of forensic importance
- Wells, J D, Lunt, N, Villet, Martin H
- Authors: Wells, J D , Lunt, N , Villet, Martin H
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6888 , http://hdl.handle.net/10962/d1011648
- Description: Chrysomya chloropyga (Wiedemann) and C. putoria (Wiedemann) (Diptera: Calliphoridae) are closely related Afrotropical blowflies that breed in carrion and latrines, reaching high density in association with humans and spreading to other continents. In some cases of human death, Chyrsomya specimens provide forensic clues. Because the immature stages of such flies are often difficult to identify taxonomically, it is useful to develop DNA-based tests for specimen identification. Therefore we attempted to distinguish between C. chloropyga and C. putoria using mitochondrial DNA (mtDNA) sequence data from a 593-bp region of the gene for cytochrome oxidase subunit one (COI). Twelve specimens from each species yielded a total of five haplotypes, none being unique to C. putoria. Therefore it was not possible to distinguish between the two species using this locus. Maximum parsimony analysis indicated paraphyletic C. chloropyga mtDNA with C. putoria nested therein. Based on these and previously published data, we infer that C. putoria diverged very recently from C. chloropyga.
- Full Text:
- Date Issued: 2004
- Authors: Wells, J D , Lunt, N , Villet, Martin H
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:6888 , http://hdl.handle.net/10962/d1011648
- Description: Chrysomya chloropyga (Wiedemann) and C. putoria (Wiedemann) (Diptera: Calliphoridae) are closely related Afrotropical blowflies that breed in carrion and latrines, reaching high density in association with humans and spreading to other continents. In some cases of human death, Chyrsomya specimens provide forensic clues. Because the immature stages of such flies are often difficult to identify taxonomically, it is useful to develop DNA-based tests for specimen identification. Therefore we attempted to distinguish between C. chloropyga and C. putoria using mitochondrial DNA (mtDNA) sequence data from a 593-bp region of the gene for cytochrome oxidase subunit one (COI). Twelve specimens from each species yielded a total of five haplotypes, none being unique to C. putoria. Therefore it was not possible to distinguish between the two species using this locus. Maximum parsimony analysis indicated paraphyletic C. chloropyga mtDNA with C. putoria nested therein. Based on these and previously published data, we infer that C. putoria diverged very recently from C. chloropyga.
- Full Text:
- Date Issued: 2004
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