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Conceptualisations and pedagogical practices of academic literacy in Namibian higher education

- Julius, Lukas Homateni

Authors: Julius, Lukas Homateni
Date: 2021-04
Subjects: Information literacy -- Study and teaching (Higher) -- Namibia , Academic writing -- Study and teaching -- Namibia , Qualitative research -- Methodology , Academic language -- Namibia , Information literacy -- Social aspects
Language: English
Type: thesis , text , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/177293 , vital:42807 , 10.21504/10962/177293
Description: The purpose of this study was to investigate academic literacy development lecturers’ conceptualisations of academic literacy and resultant pedagogical practices in academic development courses at three different Higher Education Institutional types in Namibia. The research sites were a Traditional University, a University of Technology and a Comprehensive University. The focus was to understand the extent to which the academics’ conceptions of academic literacy and the resultant pedagogical practices in the academic development courses at these three Higher Education Institutions (HEIs) facilitate epistemological access into students’ chosen fields of study. Bernstein’s Pedagogical theory (1990), Genre theory (1996) and Halliday’s Systemic Functional Linguistics (1978) were used as the study’s theoretical lenses and analytical framework. An interpretative paradigm and a qualitative case study design were employed as the research approach. Semi-structured interviews, classroom observations and documentary evidence were used to generate data. Research findings revealed a common (mis)conception of the nature of academic literacy, the resultant inadequate learning support offered to students in the selected academic literacy development courses, and a clear divorcing of academic literacy interventions from the students’ ‘home’ or mainstream disciplines at the three HEIs. The participants understood academic literacy from an autonomous position as a set of generic skills which could be taught outside of mainstream classes. Moreover, findings revealed that this understanding impacted on the design and assessments of all the academic literacy courses across the three universities under study. The study calls for a context sensitive model through which academic literacy acquisition can be scaffolded to meet the discipline-specific epistemological needs of the students. , Elalakano lyehokololoningomwa lyomapekapeko ndika olyo okukonakona ehumithokomeho lyomikalo dhokulesha nokushanga meilongngo lyopombada (oAcademic Literaci) maaputudhilongi, okukonakona omafatululo giisimanintsa moAcademic Literaci osho wo okutala iizemo yomikalo dhayooloka dhokulonga noku ilonga iilongwa yayooloka miiputudhilo yelongo lyopombada moNamibia. Omapekapeko ngaka oga li ga ningilwa miiputudhilo yomaukwatya ta ga landula; Oshiputudhiilo shopamudhigululwakalo, Oshiputudhilo shopaunongononi, nOshiputudilo shomailongo gaandjakana. Oshintsa shopokati shomapekapeko ngaka osho okuuva ko ondodo yowino osho wo euveko lyoAcademic Literaci maaputudhilongi nonkene euveko nontseyo ndjika tayi longithwa oku eta oshizemo tashi humitha komeho euveko lyopombanda lyaalongwa yomailongo geewino dhayooloka miiputudhilo itatu yelongo lyopombanda; shino otashi kwathele aalongwa yamone ontseyo ndjoka tayi ya kwathele meilongo lyawo. Omapekapeko ngano oga longitha omadhiladhiloukithi (eetheori) ga Bernstein’s Pedagogical theori (1990), Genre theori (1996) na Halliday’s Systemic Functional Linguistics theori (1978), mokufatulula nokundjandjukununa iizemo yomapekapeko. Omodela yokukonakona iizemo yongushu tayi ziilile maakonakonwa, oya tala ekonakono ndika onga oshintsa shopokati, oyo ya longithwa, opo ku monike uuyelele wothaathaa. Omikalo dha longifwa mokukonakona noku gongela uuyelele momapekapeko ngano ongaashi, eenkundathana dhayaali, omatalelo geetundi oshoyo omakonakono giinyanyangidhwa tayi kwandjangele nepekapeko ndika. Iizedjemo yepekapeko ndika otayi ulike kutya opena engwangwano montseyo nenge mefatululo lyuukwatya woAcademic Literaci, shoka sha eta enkundipalo meyambidhidho hali pewa aalongwa miilongwa yeewino dhayooloka. Shika otashi ulike kutya kapena etsokumwe pokati keenkambadhala tadhi ningwa kaapudhilongi dhokulonga oAcademic Litraci miilongwa ya yooloka mbyoka tayi ilongelwa kaalongwa miiputudhilo itatu yopombada. iizemmo yepekapeko olyo tuu mdika oya ulike wo kutya aalongwa mboka yaza komailongo ga yooloka oha yi ilongo nuudhigu opo ya pondole ondondo yomadhiladhilo gopombanda meilongo lyuukumwe. Mokukonakona euveko lyoAcademic Literaci, epekapeko ndika olya ndhindhilike kutya aakuthimbinga oyena euveko lyankundipala lyoterma ‘Academic Literaci,’ ano ya nyengwa okukwatakanitha oohedi dhopetameko ndhoka dhina oku ilongwa meikalekelo - ano pondje yiilongwa ikwao. Oshikwao, iizemo oya ulike kutya euveko ndika otali nwetha mo etungepo lyoAcademic Literaci onga oshilongwa, osho wo omakonakono gasho miiputudilo yombombanda itatu yakwatelwa momapekapeko. Hugunina, epekapeko ndika otali ulike/gandja oshiholelwa shomodela ndjoka oAcademic literacy tai vulu okulongwa opo yi kwatelemo eilongo lyiikwatelela kiilongwa osho yo komaitaalo nokeempumbwe dhaalongwa miiputudhilo yopombabda. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning (CHERTL), 2021
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Date Issued: 2021-04

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Ritualised discourse practices of feedback in a university foundation programme: a critical investigation

- Richardson, Amy

Authors: Richardson, Amy
Date: 2012
Subjects: Uncatalogued
Language: English
Type: thesis , text , Masters , MA
Identifier: http://hdl.handle.net/10962/164955 , vital:41187
Description: In order for students to become true members of academic communities of practice they need epistemological access, including guidance on central institutional knowledge-producing processes and mastery of key academic literacy practices. A powerful source of guidance is marker feedback. Drawing on key insights from the New Literacy Studies and taking up the mandate of Critical Ethnography to improve the status quo, this thesis reports on the feedback practices in a university foundation programme. The findings are based on three micro-case studies compiled and analysed by means of methods drawn from Ethnography, Sociolinguistics and Critical Analysis in conjunction with an expanded, multimodal, APPRAISAL analysis, including adjusted categories and the author’s own feedback typologies. Two major arguments emerge: the feedback provided amounts to a set of ritualised discourse practices and its effects can be likened to the product of the children’s game of Head-body-tail. Consequently, feedback conventions are opaque and, potentially, impede epistemological access. They further entrench five sets of ideologies: (1) Students must master basic English literacy before they are coached in more complex issues such as argumentation; an assumption which leads to differential socialisation. (2) There is a single set of literacy practices that is rewarded. (3) Students have different levels of authorial authority depending on their language abilities. (4) ‘Middle students’ may benefit the most from feedback. (5) Specific comments are preferred over general ones. Analysis of feedback, furthermore, shows that markers’ frames of reference shape their pedagogy and that draft and final versions of work may be framed differently by markers. These findings require a response and, in order to facilitate epistemological access, suggestions are made for improved marker training based on the problematisation of the ritual involved in marking students’ work in this university foundation programme. , Thesis (MA)--Rhodes University, Faculty of Humanities, Linguistics, 2012
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Date Issued: 2012

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A Systematic Visualisation Framework for Radio-Imaging Pipelines

- Andati, Lexy Acherwa Livoyi

Authors: Andati, Lexy Acherwa Livoyi
Date: 2021-04
Subjects: Radio interferometers , Radio astronomy -- Data processing , Radio astronomy -- Data processing -- Software , Jupyter
Language: English
Type: thesis , text , Masters , MSc
Identifier: http://hdl.handle.net/10962/177338 , vital:42812
Description: Pipelines for calibration and imaging of radio interferometric data produce many intermediate images and other data products (gain tables, etc.) These often contain valuable information about the quality of the data and the calibration, and can provide the user with valuable insights, if only visualised in the right way. However, the deluge of data that we’re experiencing with modern instruments means that most of these products are never looked at, and only the final images and data products are examined. Furthermore, the variety of imaging algorithms currently available, and the range of their options, means that very different results can be produced from the same set of original data. Proper understanding of this requires a systematic comparison that can be carried out both by individual users locally, and by the community globally. We address both problems by developing a systematic visualisation framework based around Jupyter notebooks, enriched with interactive plots based on the Bokeh and Datashader visualisation libraries. , Thesis (MSc) -- Faculty of Science, Department of Physics and Electronics, 2021
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Date Issued: 2021-04

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In silico identification of selective novel hits against the active site of wild type mycobacterium tuberculosis pyrazinamidase and its mutants

- Gowo, Prudence

Authors: Gowo, Prudence
Date: 2021-04
Subjects: Mycobacterium tuberculosis , Pyrazinamide , Multidrug resistance , Antitubercular agents , Molecular dynamics , Hydrogen bonding , Ligand binding (Biochemistry) , Dynamic Residue Network
Language: English
Type: thesis , text , Masters , MSc
Identifier: http://hdl.handle.net/10962/178007 , vital:42898
Description: The World Health Organization declared Tuberculosis a global health emergency and has set a goal to eradicate it by 2035. However, effective treatment and control of the disease is being hindered by the emerging Multi-Drug Resistant and Extensively Drug Resistant strains on the most effective first line prodrug, Pyrazinamide (PZA). Studies have shown that the main cause of PZA resistance is due to mutations in the pncA gene that codes for the target protein Pyrazinamidase (PZase). Therefore, this study aimed to identify novel drug compounds that bind to the active site of wild type PZase and study the dynamics of these potential anti-TB drugs in the mutant systems of PZase. This approach will aid in identifying drugs that may be repurposed for TB therapy and/or designed to counteract PZA resistance. This was achieved by screening 2089 DrugBank compounds against the whole wild type (WT) PZase protein in molecular docking using AutoDOCK4.2. Compound screening based on docking binding energy, hydrogen bonds, molecular weight and active site proximity identified 47 compounds meeting all the set selection criteria. The stability of these compounds were analysed in Molecular Dynamic (MD) simulations and were further studied in PZase mutant systems of A3P, A134V, A146V, D8G, D49A, D49G, D63G, H51P, H137R, L85R, L116R, Q10P, R140S, T61P, V139M and Y103S. Generally, mutant-ligand systems displayed little deviation from the WT systems. The compound systems remained compact, with less fluctuations and more hydrogen bond interactions throughout the simulation (DB00255, DB00655, DB00672, DB00782, DB00977, DB01196, DB04573, DB06414, DB08981, DB11181, DB11760, DB13867, DB13952). From this research study, potential drugs that may be repurposed for TB therapy were identified. Majority of these drugs are currently used in the treatment of hypertension, menopause disorders and inflammation. To further understand the mutant-ligand dynamic systems, calculations such as Dynamic Residue Network (DRN) may be done. Also, the bioactivity of these drugs on Mycobacterium tuberculosis may be studied in wet laboratory, to understand their clinical impart in vivo experiments. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
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Date Issued: 2021-04

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Constraining simulation uncertainties in a hydrological model of the Congo River Basin including a combined modelling approach for channel-wetland exchanges

- Kabuya, Pierre Mulamba

Authors: Kabuya, Pierre Mulamba
Date: 2021-04
Subjects: Congo River Watershed , Watersheds -- Congo (Democratic Republic) , Hydrologic models , Rain and rainfall -- Mathematical models , Runoff -- Mathematical models , Wetland hydrology
Language: English
Type: thesis , text , Doctoral , PhD
Identifier: http://hdl.handle.net/10962/177997 , vital:42897 , 10.21504/10962/177997
Description: Compared to other large river basins of the world, such as the Amazon, the Congo River Basin appears to be the most ungauged and less studied. This is partly because the basin lacks sufficient observational hydro-climatic monitoring stations and appropriate information on physiographic basin properties at a spatial scale deemed for hydrological applications, making it difficult to estimate water resources at the scale of sub-basins (Chapter 3). In the same time, the basin is facing the challenges related to rapid population growth, uncontrolled urbanisation as well as climate change. Adequate quantification of hydrological processes across different spatial and temporal scales in the basin, and the drivers of change, is essential for prediction and strategic planning to ensure sustainable management of water resources in the Congo River Basin. Hydrological models are particularly important to generate the required information. However, the shortness of the available streamflow records, lack of spatial representativeness of the available streamflow gauging stations and the lack of understanding of the processes in channel-wetland exchanges, are the main challenges that constrain the use of traditional approaches to models development. They also contribute to increased uncertainty in the estimation of water resources across the basin (Chapter 1 and 2). Given this ungauged nature of the Congo River Basin, it is important to resort to hydrological modelling approaches that can reasonably quantify and model the uncertainty associated with water resources estimation (Chapter 4) to make hydrological predictions reliable. This study explores appropriate methods for hydrological predictions and water resources assessment in ungauged catchments of the Congo River Basin. In this context, the core modelling framework combines the quantification of uncertainty in constraint indices, hydrological modelling and hydrodynamic modelling. The latter accounts for channel-wetland exchanges in sub-basins where wetlands exert considerable influence on downstream flow regimes at the monthly time scale. The constraint indices are the characteristics of a sub-basin’s long-term hydrological behaviour and may reflect the dynamics of the different components of the catchment water balance such as climate, water storage and different runoff processes. Currently, six constraint indices namely the mean monthly runoff volume (MMQ in m3 *106), mean monthly groundwater recharge depth (MMR in mm), the 10th, 50th and 90th percentiles of the flow duration curve expressed as a fraction of MMQ (Q10/MMQ, Q50/MMQ, Q90/MMQ) and the percentage of time that zero flows are expected (%Zero), are used in the modelling approach. These were judged to be the minimum number of key indices that can discriminate between different hydrological responses. The constraint indices in the framework help to determine an uncertainty range within which behavioural model parameters of the expected hydrological response can be identified. Predictive equations of the constraint indices across all climate and physiographic regions of the Congo Basin were based only on the aridity index because it was the most influential sub-basin attribute (Chapter 5) for which quantitative information was available. The degree of uncertainty in the constraint Q10/MMQ and Q50/MMQ indices is less than 41%, while it is somewhat higher for the mean monthly runoff (MMQ) and Q90/MMQ constraint indices. The established uncertainty ranges of the constraint indices were tested in some selected sub-basins of the Congo Basin, including the Lualaba (93 sub-basins), Sangha (24 sub-basins), Oubangui (19 sub-basins), Batéké plateaux (4 sub-basins), Kasai (4 sub-basins) and Inkisi (3 sub-basins). The results proved useful through the application of a 2-stage uncertainty approach of the PITMAN model. However, it comes out of this study that the application of the original constraint indices ranges (Chapter 5) generated satisfactory simulation results in some areas, while in others both small and large adjustments were required to fully capture some aspects of the observed hydrological responses (Chapter 6). Part of the reason is attributed to the availability and quality of streamflow data used to develop the constraint indices ranges (Chapter 5). The main issue identified in the modelling process was whether the changes made to the original constraints at headwater-gauged sub-basins can be applied to ungauged upstream sub-basins to match the observed flow at downstream gauging stations. Ideally, only gauged sub-basin’s constraints can be easily revised based on the observed flow. However, the refinement made to gauged sub-basins alone may fail to substantially affect the results if ungauged upstream sub-basins exert a major impact on defining downstream hydrological response. The majority of gauging stations used in this analysis are located downstream of many upstream ungauged sub-basins and therefore adjustments were required in ungauged sub-basins. These adjustments consist of shifting the full range of a constraint index either towards higher or lower values, depending on the degree to which the simulated uncertainty bounds depart from the observed flow. While this modelling approach seems effective in capturing many aspects of the hydrological responses with a reduced level of uncertainty compared to a previous study, it is recommended that the approach be extended to the remaining parts of the Congo Basin and assessed under current and future development conditions including environmental changes. A 2D hydrodynamic river-wetland model (LISFLOOD-FP) has been used to explicitly represent the inundation process exchanges between river channels and wetland systems. The hydrodynamic modelling outputs are used to calibrate the PITMAN wetland sub-model parameters. The five hydrodynamic models constructed for Ankoro, Kamalondo, Kundelungu, Mweru and Tshiangalele wetland systems have been partially validated using independent estimates of inundation extents available from Landsat imagery. Other sources of data such as remote sensing of water level altimetry, SAR images and wetland storage estimates may be used to improve the validation results. However, the important objective in this study was to make sure that flow volume exchanges between river channels and their adjacent floodplains were being simulated realistically. The wetland sub-model parameters are calibrated in a spreadsheet version of the PITMAN wetland routine to achieve visual correspondence between the LISFLOOD-FP and PITMAN wetland sub-model outputs (Storage volumes and channel outputs). The hysteretic patterns of the river-wetland processes were quantified using hysteresis indices and were associated with the spill and return flow parameters of the wetland sub-model and eventually with the wetland morphometric characteristics. One example is the scale parameter of the return flow function (AA), which shows a good relationship with the average surface slope of the wetland when the coefficient parameter (BB) of the same function is kept constant to a value of 1.25. The same parameter (AA) is a good indicator of the wetland emptying mechanism. A small AA indicates a wetland that slowly releases its flow, resulting in a highly delayed and attenuated hydrological response in downstream sub-basins. This understanding has a practical advantage for the estimation of the PITMAN wetland parameters in the many areas where it is not possible, or where the resources are not available, to run complex hydrodynamic models (Chapter 7). The inclusion of these LISFLOOD-FP informed wetland parameters in the basin-scale hydrological modelling results in acceptable simulations for the lower Lualaba drainage system. The small wetlands, like Ankoro and Tshiangalele, have a negligible impact on downstream flow regimes, whereas large wetlands, such as Kamalondo and Mweru, have very large impacts. In general, the testing of the original constraint indices in the region of wetlands and further downstream of the Lualaba drainage system has shown acceptable results. However, there remains an unresolved uncertainty issue related to the under and over-estimation of some aspects of the hydrological response at both Mulongo and Ankoro, two gauging stations in the immediate downstream of the Kamalondo wetland system. It is difficult to attribute this uncertainty to Kamalondo wetland parameters alone because many of the incremental sub-basins contributing to wetland inflows are ungauged. The issue at Mulongo is the under simulation of low flow, while the high flows at the Ankoro gauging station are over-simulated. However, the pattern of the calibrated constraint indices in this region (Chapter 8) shows that the under simulation of low flow at Mulongo cannot be attributed to incremental sub-basins (between Bukama, Kapolowe and Mulongo gauging stations), because their Q90/MMQ constraint indices are even slightly above the original constraint ranges, but maintain a spatial consistency with sub-basins of other regions. Similarly, sub-basins located between Mulongo, Luvua and Ankoro gauging stations have high flow indices slightly below the original constraint ranges and therefore they are unlikely to be responsible for the over simulation of high flow at the Ankoro gauging station. These facts highlight the need for a further understanding of the complex wetland system of Kamalondo. Short-term data collection and monitoring programme are required. Important tributaries that drain to this wetland need to be monitored by installing water level loggers and periodically collecting flow data and river bathymetry. This programme should lead to the development of rating curves of wetland input tributaries. This would partially solve the unresolved uncertainty issues at the Ankoro and Mulongo gauging stations. The integrated modelling approach offers many opportunities in the Congo Basin. The quantified and modelled uncertainty helps to identify regions with high uncertainty and allows for the identification of various data collection and management strategies that can potentially contribute to the uncertainty reduction. The quantified channel-wetland exchanges contribute to the improvement of the overall knowledge of water resources estimation within the regions where the effects of wetlands are evident even at the monthly time scale. In contrast, ignoring uncertainty in the estimates of water resources availability means that water resources planning and management decisions in the Congo Basin will continue to be based on inadequate information and unquantified uncertainty, thus increasing the risk associated with water resources decision making. , Thesis (PhD) -- Faculty of Science, Institute for Water Research, 2021
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Date Issued: 2021-04

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Physical activity, physical fitness, sedentary behaviour and screen time profiles of primary school children in a school within the Makana Municipality, Eastern Cape, South Africa

- Kade, Andréa Gaby

Authors: Kade, Andréa Gaby
Date: 2021-04
Subjects: Sedentary behavior in children -- South Africa -- Makana Municipality , Physical fitness for children -- South Africa -- Makana Municipality , Exercise -- Physiological aspects , Exercise -- Health aspects , Diabetes -- Risk factors , Heart -- Diseases -- Risk factors
Language: English
Type: thesis , text , Masters , MSc
Identifier: http://hdl.handle.net/10962/177282 , vital:42806
Description: There is a lack of data on the physical activity, physical fitness and sedentary behaviour of children in South Africa. Furthermore chronic diseases such as type II diabetes and coronary heart disease have been linked to childhood inactivity, and improving cardiorespiratory fitness and increasing levels of physical activity has been shown to be beneficial in reducing the risk of such diseases. In South Africa more broadly there is a lack of information on levels of physical activity and sedentary behaviour as well as physical fitness measures particularly in children. Objective: The purpose of this study was to enhance the existing body of knowledge on this, by measuring the prevalence of physical inactivity, sedentary behaviour and screen time of primary school children in a private school from the Makana Municipal region in the Eastern Cape Province of South Africa, where the evidence is most sparse. A secondary aim was to assess levels of physical fitness in this cohort, as well as to compare across grades and sexes. Method: After ethical approval was granted, stature and body mass measurements were taken and body mass index was computed for primary school children (Grade one to seven) from a private school in the Makana Municipality. Sitting stature was measured in order to determine each child’s peak height velocity and maturity offset. Five different physical fitness tests were conducted on the children and blood pressure and heart rate values were measured at rest. Physical activity and screen time questionnaires were completed by the children’s parents for a seven day period. Results: Females were found to mature faster than males. In addition females were more physically active (Females= 916 min/week; Males= 669 min/week) while also accumulating more sedentary time (Females= 1241 min/week; Males= 970 min/week) as compared to males, although no significant differences were found between males and females for these variables. The sample participated in more than 60 minutes of moderate-to-vigorous physical activity and less than two hours of screen time daily, which is in accordance with guidelines. No differences were found between grades and sexes for accumulated screen time. Strength increased significantly with grade and age, and there were also statistical differences between the sexes for all three strength measures with males being stronger than females. Females were more flexible than males and no statistical differences were found between sexes for the shuttle run test. Blood pressure correlated positively with mass, as did all the strength measures as well as maturity offset. No correlations were found between screen time and all other variables. A negative relationship was recorded between screen time and physical activity, although not significant. For females a strong positive correlation was found between sedentary behaviour and physical activity. Conclusion: The study was successful in adding to the body of knowledge regarding the prevalence of physical inactivity, sedentary behaviour and screen time within school children from the Makana Municipality in the Eastern Cape Province of South Africa. The sample was considered physically active as they accumulated on average more than 60 minutes of moderate-to-vigorous physical activity daily (Males= 96 min/day; Females 130 min/day), which is in accordance with guidelines recommended by the World Health Organization (World Health Organization, 2018). The sample accumulated more sedentary behaviour than physical activity, although not significant. Screen time was below regulations of two hours daily, indicating this population participated in more non screen related sedentary behaviour. More research is needed into the risks presented with different forms of sedentary behaviour. Physical fitness increased with increasing grade and age and males were considerably more fit. The current study did not present any concern with regards to the effects of increased physical activity and fitness levels within school aged children. More research into the current levels of physical activity, physical fitness and sedentary behaviour of school aged children in South Africa in general, and in the Eastern Cape Province, is needed. , Thesis (MSc) -- Faculty of Science, Human Kinetics and Ergonomics, 2021
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Date Issued: 2021-04

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Selection for improved virulence of Cryptophlebia peltastica nucleopolyhedrovirus (CrpeNPV) to False Codling Moth, Thaumatotibia leucotreta, by serial passage through a heterologous host

- Iita, Petrus Paulus

Authors: Iita, Petrus Paulus
Date: 2021-04
Subjects: Cryptophlebia leucotreta -- Biological control , Biological pest control agents , Citrus -- Diseases and pests , Baculoviruses , Cryptophlebia peltastica nucleopolyhedrovirus (CrpeNPV)
Language: English
Type: thesis , text , Masters , MSc
Identifier: http://hdl.handle.net/10962/178180 , vital:42918
Description: The false codling moth (FCM), Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) is endemic to southern Africa, and strongly associated with citrus. As South African citrus production is mainly for export to foreign markets, the market access risk due to the phytosanitary status of this pest is considerable and its control is therefore imperative. Various control measures as part of a rigorous integrated pest management (IPM) programme targeted against T. leucotreta have been effective at suppressing the pest in citrus, but there is still a growing need for continued improvement of the programme and augmentation of the available control options. Of these control options, biological control, particularly the use of Cryptophlebia leucotreta granulovirus (CrleGV-SA), is a key component of IPM in citrus orchards and it has been very successful at reducing T. leucotreta populations in the field for almost two decades. There is however, a growing need for more baculovirus variants with an improved virulence against T. leucotreta for a more efficient pest management system. The newly identified insect virus, Cryptophlebia peltastica nucleopolyhedrovirus (CrpeNPV) offers a unique opportunity for an additional biopesticide in IPM for control of T. leucotreta in the field. This study aimed to conduct serial passaging of CrpeNPV through a heterologous host, T. leucotreta, in order to determine the potential for improved virulence or speed of kill against it. In order to select for a variant of CrpeNPV with improved virulence against T. leucotreta, a high dose (LC90) of the virus OBs was used to perform 12 serial passages through T. leucotreta larvae in surface-dose bioassays. Whole genome sequencing and analysis of the passaged virus, along with restriction endonuclease profiling in silico was performed to determine if the genetic identity of the virus had changed during serial passage, in relation to the original virus. These analyses indicated that the dominant genotype of CrpeNPV was maintained following 12 serial passages through the heterologous host. The biological activity of the passaged virus, along with the original virus was evaluated against neonate T. leucotreta in surface-dose bioassays and compared. Results from dose-response bioassays showed that the virulence of CrpeNPV did not improve after 12 serial passages. The LC50 values of the passaged virus and the original virus were estimated at 1.96 × 104 and 1.58 × 104 OBs/ml, respectively, whereas the LC90 values were estimated at 3.46 × 104 OBs/ml for the passaged virus and 3.68 × 104 for the original virus. Similarly, the results from time-response bioassays showed that the speed of kill of CrpeNPV did not improve after 12 serial passages. The LT50 values of the passaged virus and the original virus were 88.44 hours (3 days and 16 hours) and 83.74 hours (3 days and 12 hours), respectively, whereas the LT90 values were 115 hours (4 days 19 hours) for the passaged virus and 102 hours (4 days 6 hours) for the original virus. The virulence and speed of kill of the passaged virus decreased significantly, in relation to the original virus. When the full genome of the passaged virus was sequenced and analysed, only a few SNPs were detected in the viral genome, in comparison to the original virus. No detectable difference in REN digestion patterns were observed following REN analysis of gDNA of the passaged virus with several restriction enzymes in silico. The results for this study suggest that CrpeNPV may already be optimally suited to the heterologous host as it persists under these conditions without significant changes to the genome. These results have positive implications for the genetic integrity of CrpeNPV as a potential biocontrol agent in the field. This study is the first to report the virulence selection of CrpeNPV by serial passage through a heterologous host, and also the first to record bioassay data in terms of dose response (or lethal concentration) against T. leucotreta second instars. The data obtained have added to the knowledge about interactions between CrpeNPV and its heterologous host, and may be fundamental to continued investigation into the effect of serial passage on pathogenicity and genetic diversity of CrpeNPV. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2021
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Date Issued: 2021-04

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