Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
Epidemic transmission of intestinal schistosomiasis in the seasonal part of the Okavango Delta, Botswana:
- Appleton, C C, Ellery, William F N, Byskov, Jens, Mogkweetsinyana, S S
- Authors: Appleton, C C , Ellery, William F N , Byskov, Jens , Mogkweetsinyana, S S
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144364 , vital:38339 , DOI: 10.1179/136485908X311867
- Description: A well documented epidemic of human intestinal schistosomiasis caused by Schistosoma mansoni occurred at Maun in the seasonal part of the Okavango Delta, Botswana, building from very few cases in the 1950s and early 1960s to a peak prevalence of greater than80% in the 1980s. A retrospective analysis was performed on all available records of the prevalence of S. mansoni in the Maun area and the corresponding flow records of the Thamalakane River. These revealed a statistically significant correlation between prevalence and flow, but only when a lag period was introduced. The correlation was greatest with a lag period of 5–6 years between the rise and fall of discharge and the rise and fall of transmission. Since the hydrological events in the delta follow a cyclical pattern, another epidemic around 2020 appears likely.
- Full Text:
- Date Issued: 2013
- Authors: Appleton, C C , Ellery, William F N , Byskov, Jens , Mogkweetsinyana, S S
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144364 , vital:38339 , DOI: 10.1179/136485908X311867
- Description: A well documented epidemic of human intestinal schistosomiasis caused by Schistosoma mansoni occurred at Maun in the seasonal part of the Okavango Delta, Botswana, building from very few cases in the 1950s and early 1960s to a peak prevalence of greater than80% in the 1980s. A retrospective analysis was performed on all available records of the prevalence of S. mansoni in the Maun area and the corresponding flow records of the Thamalakane River. These revealed a statistically significant correlation between prevalence and flow, but only when a lag period was introduced. The correlation was greatest with a lag period of 5–6 years between the rise and fall of discharge and the rise and fall of transmission. Since the hydrological events in the delta follow a cyclical pattern, another epidemic around 2020 appears likely.
- Full Text:
- Date Issued: 2013
Uptake of liposomes into bacterial cells
- Authors: Oidu, Benjamin
- Date: 2013
- Subjects: Liposomes , Drug carriers (Pharmacy)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10165 , http://hdl.handle.net/10948/d1021010
- Description: Liposomes are small phospholipid vesicles that have been widely investigated as drug carriers for the delivery of therapeutic agents. A variety of liposome formulations are presently under clinical trial exploration, while others have already been approved for clinical use. The aim of this study was to optimize liposome uptake into bacterial cells. Both gram-positive and gram-negative bacteria were used in the study as well as Candida albicans.Response surface methodology (RSM) using a central composite design (CCD) model was used to optimize liposomal formulations of carboxyfluorescien (CF) for each of the three microbes, and also the three microbes in combination namely; Staphylococcus aureus (Sa), Escherichia coli (Ec) and Candida albicans (Ca). Percentage of CF encapsulated and CF increase in Uptake were investigated with respect to two independent variables that were, cholesterol (CHOL) and stearylamine (SA) content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each microbe and the three microbes in combination. The model selected by the software managed to reasonably correlate the predicted models to the experimental data. Encapsulation of carboxyfluorescien (CF) into a liposome formulation enhanced its uptake by Staphylococcus aureus and Escherichia coli as well as Candida albicans. This was evident in the increase in CF uptake when the uptake rate of free CF was compared with that of liposomal CF.
- Full Text:
- Date Issued: 2013
- Authors: Oidu, Benjamin
- Date: 2013
- Subjects: Liposomes , Drug carriers (Pharmacy)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10165 , http://hdl.handle.net/10948/d1021010
- Description: Liposomes are small phospholipid vesicles that have been widely investigated as drug carriers for the delivery of therapeutic agents. A variety of liposome formulations are presently under clinical trial exploration, while others have already been approved for clinical use. The aim of this study was to optimize liposome uptake into bacterial cells. Both gram-positive and gram-negative bacteria were used in the study as well as Candida albicans.Response surface methodology (RSM) using a central composite design (CCD) model was used to optimize liposomal formulations of carboxyfluorescien (CF) for each of the three microbes, and also the three microbes in combination namely; Staphylococcus aureus (Sa), Escherichia coli (Ec) and Candida albicans (Ca). Percentage of CF encapsulated and CF increase in Uptake were investigated with respect to two independent variables that were, cholesterol (CHOL) and stearylamine (SA) content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each microbe and the three microbes in combination. The model selected by the software managed to reasonably correlate the predicted models to the experimental data. Encapsulation of carboxyfluorescien (CF) into a liposome formulation enhanced its uptake by Staphylococcus aureus and Escherichia coli as well as Candida albicans. This was evident in the increase in CF uptake when the uptake rate of free CF was compared with that of liposomal CF.
- Full Text:
- Date Issued: 2013
Polyunsaturated fatty acid metabolism and effects on colon cancer cell biology in vitro.
- Authors: Bulcao, Candice
- Date: 2013
- Subjects: Unsaturated fatty acids , Unsaturated fatty acids in human nutrition , Colon (Anatomy)-- Cancer , Cancer -- Nutritional aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4137 , http://hdl.handle.net/10962/d1016128
- Description: Colon cancer is a leading cause of cancer related deaths worldwide. Lifestyle factors such as diet and exercise have been implicated as important agents in colon cancer development and progression. Epidemiological, in vivo and in vitro studies have found that n-3 polyunsaturated fatty acids (PUFAs) reduce colon carcinoma. The role of n-6 PUFAs remains a controversial topic, with studies indicating both promoting and preventing capabilities published. In order to better understand the effects of PUFAs on colon carcinoma, it is important to have an understanding of how they will be broken down in the body. During this study, in silico metabolism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) predicted the formation of hydroxy-, di-hydroxy- and epoxy-FAs. A gas chromatography-mass spectrometry method was developed and validated for the detection of these PUFAs and their cytochrome P450 (CYP) metabolites. A human liver microsomal system for the in vitro metabolism of EPA, DHA and AA was optimised in terms of microsomal and PUFA concentration. The system resulted in the metabolism of the positive control, lauric acid, to 12-hydroxy-lauric acid but was unable to metabolise the PUFAs of interest. EPA, DHA and AA reduced cell viability in the colon carcinoma cell lines SW480 and SW620 in the micromolar concentration range (25 – 200 μM). The CYP epoxidation metabolite of EPA, 17, 18-epoxyeicosatetraenoic acid (17, 18-EpETE) resulted in a significant reduction in SW480 cell viability relative to the parent compound at lower concentrations (25 and 50 μM). Annexin V apoptosis analysis revealed that EPA and 17, 18- EpETE did not result in apoptosis in SW480 cells at a concentration of 25 μM and over an incubation period of 24 hours. A significant reduction in reactive oxygen species production was seen in SW480 cells after incubation with 25 μM 17, 18-EpETE for 24 hours. EPA and 17, 18-EpETE were implicated in the reduction of colon cancer metastasis since they were able to reduce SW480 migration and anchorage independent cell growth. These results indicate that the dietary intake of EPA, DHA and AA may be beneficial to one’s health due to the negative effects that these PUFAs had on colon carcinoma. Future studies are needed to confirm these benefits and compare the effects of the PUFAs to their CYP-metabolites.
- Full Text:
- Date Issued: 2013
- Authors: Bulcao, Candice
- Date: 2013
- Subjects: Unsaturated fatty acids , Unsaturated fatty acids in human nutrition , Colon (Anatomy)-- Cancer , Cancer -- Nutritional aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4137 , http://hdl.handle.net/10962/d1016128
- Description: Colon cancer is a leading cause of cancer related deaths worldwide. Lifestyle factors such as diet and exercise have been implicated as important agents in colon cancer development and progression. Epidemiological, in vivo and in vitro studies have found that n-3 polyunsaturated fatty acids (PUFAs) reduce colon carcinoma. The role of n-6 PUFAs remains a controversial topic, with studies indicating both promoting and preventing capabilities published. In order to better understand the effects of PUFAs on colon carcinoma, it is important to have an understanding of how they will be broken down in the body. During this study, in silico metabolism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) predicted the formation of hydroxy-, di-hydroxy- and epoxy-FAs. A gas chromatography-mass spectrometry method was developed and validated for the detection of these PUFAs and their cytochrome P450 (CYP) metabolites. A human liver microsomal system for the in vitro metabolism of EPA, DHA and AA was optimised in terms of microsomal and PUFA concentration. The system resulted in the metabolism of the positive control, lauric acid, to 12-hydroxy-lauric acid but was unable to metabolise the PUFAs of interest. EPA, DHA and AA reduced cell viability in the colon carcinoma cell lines SW480 and SW620 in the micromolar concentration range (25 – 200 μM). The CYP epoxidation metabolite of EPA, 17, 18-epoxyeicosatetraenoic acid (17, 18-EpETE) resulted in a significant reduction in SW480 cell viability relative to the parent compound at lower concentrations (25 and 50 μM). Annexin V apoptosis analysis revealed that EPA and 17, 18- EpETE did not result in apoptosis in SW480 cells at a concentration of 25 μM and over an incubation period of 24 hours. A significant reduction in reactive oxygen species production was seen in SW480 cells after incubation with 25 μM 17, 18-EpETE for 24 hours. EPA and 17, 18-EpETE were implicated in the reduction of colon cancer metastasis since they were able to reduce SW480 migration and anchorage independent cell growth. These results indicate that the dietary intake of EPA, DHA and AA may be beneficial to one’s health due to the negative effects that these PUFAs had on colon carcinoma. Future studies are needed to confirm these benefits and compare the effects of the PUFAs to their CYP-metabolites.
- Full Text:
- Date Issued: 2013
Understanding current teacher implementation of Zimbabwe's primary school AIDS curriculum: a case study
- Authors: Musingarabwi, Starlin
- Date: 2013
- Subjects: Teacher participation in curriculum planning -- Zimbabwe , Primary school teachers -- Zimbabwe , Education -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9584 , http://hdl.handle.net/10948/d1020912
- Description: Zimbabwe’s Ministry of Education Sport, Arts and Culture offers as one of the primary school curricula, an AIDS curriculum which all Grades 4 to 7 teachers in Zimbabwe’s primary schools mandatorily implement with a view to contributing towards the prevention of the spread of HIV/AIDS among the young primary school learners. The purpose of this research was to explore and describe teachers’ understanding and implementation of Zimbabwe’s primary school AIDS curriculum regarding the ways in which they articulated teaching practices and processes in their classrooms. The study also aimed to elicit the teachers’ views on how personal and contextual factors impact their adaptation and enactment of the curriculum. The study also sought to establish teachers’ perceptions of their practical experiences with the implementation of Zimbabwe’s primary school AIDS curriculum and their suggestions for improving practice. The study follows a qualitative case study design with minimal quantitative results. It involved three purposively selected primary school grade six teachers (n=3) each of whom was asked to teach five lessons while being observed over a period of three months. Each teacher availed his or her teaching scheme/plan to the researcher who conducted document analysis to glean their symbolic conceptualisation of actual classroom practice of the curriculum. This was followed by three semi-structured interviews with each participating teacher to elicit their perceptions. A content analysis using ideas borrowed from the grounded theory approach was employed resulting in thematic findings. The findings of the study confirm and enhance the theoretical significance of the phenomenological-adaptive perspective of educational change and Honig’s (people, policy, places) and cognition model for describing teacher implementation of the mandatory AIDS curriculum. The findings also confirm the complex ways in which human-generated personal and contextual factors played out in framing and shaping teachers’ personal adaptation of the mandatory AIDS curriculum. The study confirms the adaptation claim that as cognitive sense-makers, teachers mutate and enact a curriculum according to their personal subjective interpretations in the context of unique use-setting implementation realities. Although one of the participants’ understanding and practice displayed considerable comprehension of the requirements of the curriculum, the other teachers displayed an understanding of this curriculum in a superficial way, and experienced few positive experiences and several conceptual and operational constraints in its implementation. Drawing on their practical experiences with the implementation of the curriculum, teachers offered suggestions for transforming the implementation proficiency of this curriculum, which formed part of the conceptual strategy I developed for improving practice. Thus the resultant achievement of the study was a conceptual strategy that was constructed from the key findings of the study to provide educational change leaders with nuanced ideas and insights for improving practice.
- Full Text:
- Date Issued: 2013
- Authors: Musingarabwi, Starlin
- Date: 2013
- Subjects: Teacher participation in curriculum planning -- Zimbabwe , Primary school teachers -- Zimbabwe , Education -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:9584 , http://hdl.handle.net/10948/d1020912
- Description: Zimbabwe’s Ministry of Education Sport, Arts and Culture offers as one of the primary school curricula, an AIDS curriculum which all Grades 4 to 7 teachers in Zimbabwe’s primary schools mandatorily implement with a view to contributing towards the prevention of the spread of HIV/AIDS among the young primary school learners. The purpose of this research was to explore and describe teachers’ understanding and implementation of Zimbabwe’s primary school AIDS curriculum regarding the ways in which they articulated teaching practices and processes in their classrooms. The study also aimed to elicit the teachers’ views on how personal and contextual factors impact their adaptation and enactment of the curriculum. The study also sought to establish teachers’ perceptions of their practical experiences with the implementation of Zimbabwe’s primary school AIDS curriculum and their suggestions for improving practice. The study follows a qualitative case study design with minimal quantitative results. It involved three purposively selected primary school grade six teachers (n=3) each of whom was asked to teach five lessons while being observed over a period of three months. Each teacher availed his or her teaching scheme/plan to the researcher who conducted document analysis to glean their symbolic conceptualisation of actual classroom practice of the curriculum. This was followed by three semi-structured interviews with each participating teacher to elicit their perceptions. A content analysis using ideas borrowed from the grounded theory approach was employed resulting in thematic findings. The findings of the study confirm and enhance the theoretical significance of the phenomenological-adaptive perspective of educational change and Honig’s (people, policy, places) and cognition model for describing teacher implementation of the mandatory AIDS curriculum. The findings also confirm the complex ways in which human-generated personal and contextual factors played out in framing and shaping teachers’ personal adaptation of the mandatory AIDS curriculum. The study confirms the adaptation claim that as cognitive sense-makers, teachers mutate and enact a curriculum according to their personal subjective interpretations in the context of unique use-setting implementation realities. Although one of the participants’ understanding and practice displayed considerable comprehension of the requirements of the curriculum, the other teachers displayed an understanding of this curriculum in a superficial way, and experienced few positive experiences and several conceptual and operational constraints in its implementation. Drawing on their practical experiences with the implementation of the curriculum, teachers offered suggestions for transforming the implementation proficiency of this curriculum, which formed part of the conceptual strategy I developed for improving practice. Thus the resultant achievement of the study was a conceptual strategy that was constructed from the key findings of the study to provide educational change leaders with nuanced ideas and insights for improving practice.
- Full Text:
- Date Issued: 2013
Modelling stock return volatility dynamics in selected African markets
- Authors: King, Daniel Jonathan
- Date: 2013
- Subjects: Rate of return -- Africa Stocks -- Prices -- Africa Finance -- Developing countries -- Econometric models
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1051 , http://hdl.handle.net/10962/d1006452
- Description: Stock return volatility has been shown to occasionally exhibit discrete structural shifts. These shifts are particularly evident in the transition from ‘normal’ to crisis periods, and tend to be more pronounced in developing markets. This study aims to establish whether accounting for structural changes in the conditional variance process, through the use of Markov-switching models, improves estimates and forecasts of stock return volatility over those of the more conventional single-state (G)ARCH models, within and across selected African markets for the period 2002-2012. In the univariate portion of the study, the performances of various Markov-switching models are tested against a single-state benchmark model through the use of in-sample goodness-of-fit and predictive ability measures. In the multivariate context, the single-state and Markov-switching models are comparatively assessed according to their usefulness in constructing optimal stock portfolios. It is found that, even after accounting for structural breaks in the conditional variance process, conventional GARCH effects remain important to capturing the heteroscedasticity evident in the data. However, those univariate models which include a GARCH term are shown to perform comparatively poorly when used for forecasting purposes. Additionally, in the multivariate study, the use of Markov-switching variance-covariance estimates improves risk-adjusted portfolio returns when compared to portfolios that are constructed using the more conventional single-state models. While there is evidence that the use of some Markov-switching models can result in better forecasts and higher risk-adjusted returns than those models which include GARCH effects, the inability of the simpler Markov-switching models to fully capture the heteroscedasticity in the data remains problematic.
- Full Text:
- Date Issued: 2013
- Authors: King, Daniel Jonathan
- Date: 2013
- Subjects: Rate of return -- Africa Stocks -- Prices -- Africa Finance -- Developing countries -- Econometric models
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1051 , http://hdl.handle.net/10962/d1006452
- Description: Stock return volatility has been shown to occasionally exhibit discrete structural shifts. These shifts are particularly evident in the transition from ‘normal’ to crisis periods, and tend to be more pronounced in developing markets. This study aims to establish whether accounting for structural changes in the conditional variance process, through the use of Markov-switching models, improves estimates and forecasts of stock return volatility over those of the more conventional single-state (G)ARCH models, within and across selected African markets for the period 2002-2012. In the univariate portion of the study, the performances of various Markov-switching models are tested against a single-state benchmark model through the use of in-sample goodness-of-fit and predictive ability measures. In the multivariate context, the single-state and Markov-switching models are comparatively assessed according to their usefulness in constructing optimal stock portfolios. It is found that, even after accounting for structural breaks in the conditional variance process, conventional GARCH effects remain important to capturing the heteroscedasticity evident in the data. However, those univariate models which include a GARCH term are shown to perform comparatively poorly when used for forecasting purposes. Additionally, in the multivariate study, the use of Markov-switching variance-covariance estimates improves risk-adjusted portfolio returns when compared to portfolios that are constructed using the more conventional single-state models. While there is evidence that the use of some Markov-switching models can result in better forecasts and higher risk-adjusted returns than those models which include GARCH effects, the inability of the simpler Markov-switching models to fully capture the heteroscedasticity in the data remains problematic.
- Full Text:
- Date Issued: 2013
Agriculture as a contributor to local economic development (LED) in Nkonkobe Local Municipality
- Authors: Rali, Jongikhaya
- Date: 2013
- Subjects: Agricultural development projects -- South Africa -- Eastern Cape , Poverty -- Economic aspects -- South Africa -- Eastern Cape. , Job creation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9201 , http://hdl.handle.net/10948/d1020402
- Description: Africa while being underdeveloped in terms of industry and infrastructure has much rural land and therefore has significant potential to provide global food security. This study investigates agriculture as a contributor to economic development of Nkonkobe Local Municipality, Eastern Cape, South Africa. While the potential of agriculture, in Nkonkobe Local Municipality is high, this sector has not been fully developed to the benefit of the community and the municipality at large. The results of this study have pointed out that the Eastern Cape, Nkonkobe Local Municipality in particular is where communal farming is practiced on the largest scale and where further growth can take place. This study is informed by the goals of reconstruction and development, as expressed in the Government’s Reconstruction and Development Programme (1995) tabled to meet the basic needs of the people, develop its human resources and to build the local economy. This study aims to transform Nkonkobe Local Municipality into an economically developed area of Amathole District Municipality, by improving the area’s agricultural contribution to the Local Economic Development (LED) of the area. This study supports entrepreneurship and innovation, job creation, raising the quality of basic services to the poorest citizens of Nkonkobe Local Municipality, and increasing the distribution of agricultural land to small holders. This study promotes the development of agricultural sector, which will enable it to absorb more low skilled labour which will assist in poverty alleviation, and promote LED initiatives. This study also develops and promotes the sustainable contribution of agriculture to LED programmes, and improves the participation of local communities in these programmes. The study was conducted using qualitative research methodology. The findings of the study reveal that agricultural infrastructure in support of LED in Nkonkobe Local Municipality must be established and improved. Such infrastructure should include the reticulation of services, such as irrigation schemes, big dams, farming equipment, tractors, laboratories, cold storages and communication links. These services are currently inadequate in the area, and hamper development and progress that the area clearly has great potential to add in terms of global food security and poverty alleviation.
- Full Text:
- Date Issued: 2013
- Authors: Rali, Jongikhaya
- Date: 2013
- Subjects: Agricultural development projects -- South Africa -- Eastern Cape , Poverty -- Economic aspects -- South Africa -- Eastern Cape. , Job creation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9201 , http://hdl.handle.net/10948/d1020402
- Description: Africa while being underdeveloped in terms of industry and infrastructure has much rural land and therefore has significant potential to provide global food security. This study investigates agriculture as a contributor to economic development of Nkonkobe Local Municipality, Eastern Cape, South Africa. While the potential of agriculture, in Nkonkobe Local Municipality is high, this sector has not been fully developed to the benefit of the community and the municipality at large. The results of this study have pointed out that the Eastern Cape, Nkonkobe Local Municipality in particular is where communal farming is practiced on the largest scale and where further growth can take place. This study is informed by the goals of reconstruction and development, as expressed in the Government’s Reconstruction and Development Programme (1995) tabled to meet the basic needs of the people, develop its human resources and to build the local economy. This study aims to transform Nkonkobe Local Municipality into an economically developed area of Amathole District Municipality, by improving the area’s agricultural contribution to the Local Economic Development (LED) of the area. This study supports entrepreneurship and innovation, job creation, raising the quality of basic services to the poorest citizens of Nkonkobe Local Municipality, and increasing the distribution of agricultural land to small holders. This study promotes the development of agricultural sector, which will enable it to absorb more low skilled labour which will assist in poverty alleviation, and promote LED initiatives. This study also develops and promotes the sustainable contribution of agriculture to LED programmes, and improves the participation of local communities in these programmes. The study was conducted using qualitative research methodology. The findings of the study reveal that agricultural infrastructure in support of LED in Nkonkobe Local Municipality must be established and improved. Such infrastructure should include the reticulation of services, such as irrigation schemes, big dams, farming equipment, tractors, laboratories, cold storages and communication links. These services are currently inadequate in the area, and hamper development and progress that the area clearly has great potential to add in terms of global food security and poverty alleviation.
- Full Text:
- Date Issued: 2013
Investigation into the technical feasibility of biological treatment of precious metal refining wastewater
- Authors: Moore, Bronwyn Ann
- Date: 2013
- Subjects: Sewage -- Purification -- Biological treatment -- South Africa Sewage -- Purification -- Activated sludge process -- South Africa Water reuse -- South Africa Flotation -- South Africa Platinum mines and mining -- Waste disposal -- South Africa Platinum mines and mining -- Economic aspects -- South Africa Mine water -- Environmental aspects -- South Africa Platinum mines and mining -- Waste minimization -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3888 , http://hdl.handle.net/10962/d1002013
- Description: The hydrometallurgical refining of platinum group metals results in large volumes of liquid waste that requires suitable treatment before any disposal can be contemplated. The wastewater streams are characterized by extremes of pH, high inorganic ion content (such as chloride), significant residual metal loads and small amounts of entrained organic compounds. Historically these effluents were housed in evaporation reservoirs, however lack of space and growing water demands have led Anglo Platinum to consider treatment of these effluents. The aim of this study was to investigate whether biological wastewater treatment could produce water suitable for onsite reuse. Bench-scale activated sludge and anaerobic digestion for co-treatment of an acidic refinery waste stream with domestic wastewater were used to give preliminary data. Activated sludge showed better water treatment at lab scale in terms of removal efficiencies of ammonia (approximately 25%, cf. 20% in anaerobic digestion) and COD (70% cf. 43% in digestion) and greater robustness when biomass health was compared. Activated sludge was consequently selected for a pilot plant trial. The pilot plant was operated on-site and performed comparably with the bench-scale system, however challenges in the clarifier design led to losses of biomass and poor effluent quality (suspended solids washout). The pilot plant was unable to alter the pH of the feed, but a two week maturation period resulted in the pH increasing from 5.3 to 7.0. Tests on algal treatment as an alternative or follow-on unit operation to activated sludge showed it not to be a viable process. The activated sludge effluent was assessed for onsite reuse in flotation and it was found that there was no significant difference between its flotation performance and that of the process water currently used, indicating the effluent generated by the biological treatment system can be used successfully for flotation. Flotation is the method whereby minerals refining operations recover minerals of interest from ore through the addition of chemicals and aeration of the ore slurry. Target minerals adhere to the bubbles and can be removed from the process.
- Full Text:
- Date Issued: 2013
- Authors: Moore, Bronwyn Ann
- Date: 2013
- Subjects: Sewage -- Purification -- Biological treatment -- South Africa Sewage -- Purification -- Activated sludge process -- South Africa Water reuse -- South Africa Flotation -- South Africa Platinum mines and mining -- Waste disposal -- South Africa Platinum mines and mining -- Economic aspects -- South Africa Mine water -- Environmental aspects -- South Africa Platinum mines and mining -- Waste minimization -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3888 , http://hdl.handle.net/10962/d1002013
- Description: The hydrometallurgical refining of platinum group metals results in large volumes of liquid waste that requires suitable treatment before any disposal can be contemplated. The wastewater streams are characterized by extremes of pH, high inorganic ion content (such as chloride), significant residual metal loads and small amounts of entrained organic compounds. Historically these effluents were housed in evaporation reservoirs, however lack of space and growing water demands have led Anglo Platinum to consider treatment of these effluents. The aim of this study was to investigate whether biological wastewater treatment could produce water suitable for onsite reuse. Bench-scale activated sludge and anaerobic digestion for co-treatment of an acidic refinery waste stream with domestic wastewater were used to give preliminary data. Activated sludge showed better water treatment at lab scale in terms of removal efficiencies of ammonia (approximately 25%, cf. 20% in anaerobic digestion) and COD (70% cf. 43% in digestion) and greater robustness when biomass health was compared. Activated sludge was consequently selected for a pilot plant trial. The pilot plant was operated on-site and performed comparably with the bench-scale system, however challenges in the clarifier design led to losses of biomass and poor effluent quality (suspended solids washout). The pilot plant was unable to alter the pH of the feed, but a two week maturation period resulted in the pH increasing from 5.3 to 7.0. Tests on algal treatment as an alternative or follow-on unit operation to activated sludge showed it not to be a viable process. The activated sludge effluent was assessed for onsite reuse in flotation and it was found that there was no significant difference between its flotation performance and that of the process water currently used, indicating the effluent generated by the biological treatment system can be used successfully for flotation. Flotation is the method whereby minerals refining operations recover minerals of interest from ore through the addition of chemicals and aeration of the ore slurry. Target minerals adhere to the bubbles and can be removed from the process.
- Full Text:
- Date Issued: 2013
Using a unified invasion framework to characterize Africa’s first loricariid catfish invasion
- Jones, Roy W, Weyl, Olaf L F, Hill, Martin P, Swartz, Ernest R
- Authors: Jones, Roy W , Weyl, Olaf L F , Hill, Martin P , Swartz, Ernest R
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/418081 , vital:71507 , xlink:href="https://doi.org/10.1007/s10530-013-0438-7"
- Description: This paper presents evidence of establishment of a loricariid population in the Nseleni River in South Africa and uses a unified framework to determine its invasion stage. Specimens were identified morphologically as Pterygioplichthys disjunctivus (Weber 1991), but genetic barcoding results indicated close association with specimens that may have a hybrid history. The species was introduced into South Africa via the pet trade and the first record of introduction into the wild was in 2004. Samples collected in 2011 and 2012 demonstrated that there were multiple length cohorts in the population including juveniles (12–130 mm total length TL) and large (>300 mm TL) adult fish. Gonadal assessment of adults demonstrated the presence of reproduction capable specimens. The concurrent occurrence of mature adults and juvenile fish demonstrated establishment. Locality records indicate that P. disjunctivus has already spread between two rivers through an inter basin water transfer. Using a unified framework for invasions this invasion was categorized as a self-sustaining population in the wild with individuals surviving and reproducing a significant distance from their original point of introduction. Containment is suggested as potential management strategy.
- Full Text:
- Date Issued: 2013
- Authors: Jones, Roy W , Weyl, Olaf L F , Hill, Martin P , Swartz, Ernest R
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/418081 , vital:71507 , xlink:href="https://doi.org/10.1007/s10530-013-0438-7"
- Description: This paper presents evidence of establishment of a loricariid population in the Nseleni River in South Africa and uses a unified framework to determine its invasion stage. Specimens were identified morphologically as Pterygioplichthys disjunctivus (Weber 1991), but genetic barcoding results indicated close association with specimens that may have a hybrid history. The species was introduced into South Africa via the pet trade and the first record of introduction into the wild was in 2004. Samples collected in 2011 and 2012 demonstrated that there were multiple length cohorts in the population including juveniles (12–130 mm total length TL) and large (>300 mm TL) adult fish. Gonadal assessment of adults demonstrated the presence of reproduction capable specimens. The concurrent occurrence of mature adults and juvenile fish demonstrated establishment. Locality records indicate that P. disjunctivus has already spread between two rivers through an inter basin water transfer. Using a unified framework for invasions this invasion was categorized as a self-sustaining population in the wild with individuals surviving and reproducing a significant distance from their original point of introduction. Containment is suggested as potential management strategy.
- Full Text:
- Date Issued: 2013
Exploring a systems approach to mainstreaming sustainability in universities: A case study of Rhodes University in South Africa
- Togo, Muchaiteyi, Lotz-Sisitka, Heila
- Authors: Togo, Muchaiteyi , Lotz-Sisitka, Heila
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182857 , vital:43886 , xlink:href="https://doi.org/10.1080/13504622.2012.749974"
- Description: This paper explores the use of systems theory to inform the mainstreaming of sustainability in a university’s functions as it responds to sustainable development challenges in its local context. Offering a case study of Rhodes University, the paper shows how the use of systems models and concepts, underpinned by a critical realist ontology and an understanding of morphogenetic change processes, have the potential to enable universities to mobilise their operations to respond to local sustainability challenges. In this instance, the success of such an approach is shown to depend on commitments from the university community and the availability of enabling inputs, such as financial and human resources. The paper concludes with reflections and recommendations to inform further development of a newly emerging systems approach in sustainability mainstreaming at Rhodes University, and other institutions pursuing similar approaches and goals.
- Full Text:
- Date Issued: 2013
- Authors: Togo, Muchaiteyi , Lotz-Sisitka, Heila
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/182857 , vital:43886 , xlink:href="https://doi.org/10.1080/13504622.2012.749974"
- Description: This paper explores the use of systems theory to inform the mainstreaming of sustainability in a university’s functions as it responds to sustainable development challenges in its local context. Offering a case study of Rhodes University, the paper shows how the use of systems models and concepts, underpinned by a critical realist ontology and an understanding of morphogenetic change processes, have the potential to enable universities to mobilise their operations to respond to local sustainability challenges. In this instance, the success of such an approach is shown to depend on commitments from the university community and the availability of enabling inputs, such as financial and human resources. The paper concludes with reflections and recommendations to inform further development of a newly emerging systems approach in sustainability mainstreaming at Rhodes University, and other institutions pursuing similar approaches and goals.
- Full Text:
- Date Issued: 2013
Design and evaluation of an electrochemical immunosensor for measles serodiagnosis using measles-specific Immunoglobulin G antibodies
- Mashazi, Philani N, Vilakazi, Sibulelo, Nyokong, Tebello
- Authors: Mashazi, Philani N , Vilakazi, Sibulelo , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241705 , vital:50962 , xlink:href="https://doi.org/10.1016/j.talanta.2013.06.036"
- Description: The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL−1 with R2=0.997 and the limit of detection of 6.60 ng mL−1 at 3σ. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples.
- Full Text:
- Date Issued: 2013
- Authors: Mashazi, Philani N , Vilakazi, Sibulelo , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241705 , vital:50962 , xlink:href="https://doi.org/10.1016/j.talanta.2013.06.036"
- Description: The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL−1 with R2=0.997 and the limit of detection of 6.60 ng mL−1 at 3σ. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples.
- Full Text:
- Date Issued: 2013
Fluorescence “turn on” probe for bromide ion using nanoconjugates of glutathione-capped CdTe@ ZnS quantum dots with nickel tetraamino-phthalocyanine
- Adegoke, Oluwasesan, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190479 , vital:44998 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.05.013"
- Description: In this paper, three differently sized glutathione (GSH)-capped CdTe@ZnS quantum dots (QDs) have been successfully conjugated to nickel tetraamino-phthalocyanine (NiTAPc) to form different QDs-NiTAPc nanocomplexes. Several techniques such as TEM, FT-IR, time-resolved fluorescence measurement and electronic spectroscopy were employed to characterize the nanocomplex. Bromide ion was chosen as a model anion to test the efficacy of the nanoprobe. The fluorescence of the nanoconjugate was “turned off” upon binding but was progressively “turned on” upon interaction with varying concentrations of bromide ion. Experimental results showed that the quantum size effect of nanocrystal QD determined the overall sensitivity and selectivity of the nanoprobe and followed the order QD563-NiTAPc > QD605-NiTAPc > QD621-NiTAPc. The mechanism of reaction is proposed.
- Full Text:
- Date Issued: 2013
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190479 , vital:44998 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.05.013"
- Description: In this paper, three differently sized glutathione (GSH)-capped CdTe@ZnS quantum dots (QDs) have been successfully conjugated to nickel tetraamino-phthalocyanine (NiTAPc) to form different QDs-NiTAPc nanocomplexes. Several techniques such as TEM, FT-IR, time-resolved fluorescence measurement and electronic spectroscopy were employed to characterize the nanocomplex. Bromide ion was chosen as a model anion to test the efficacy of the nanoprobe. The fluorescence of the nanoconjugate was “turned off” upon binding but was progressively “turned on” upon interaction with varying concentrations of bromide ion. Experimental results showed that the quantum size effect of nanocrystal QD determined the overall sensitivity and selectivity of the nanoprobe and followed the order QD563-NiTAPc > QD605-NiTAPc > QD621-NiTAPc. The mechanism of reaction is proposed.
- Full Text:
- Date Issued: 2013
Nutrient-mediated effects on Cornops aquaticum Brüner (Orthoptera: Acrididae), a potential biological control agent of water hyacinth, Eichhornia crassipes (Mart.) Solms (Pontederiaceae)
- Bownes, Angela, Hill, Martin P, Byrne, Marcus J
- Authors: Bownes, Angela , Hill, Martin P , Byrne, Marcus J
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406131 , vital:70241 , xlink:href="https://doi.org/10.1016/j.biocontrol.2013.07.023"
- Description: Environmental nutrient availability can drive and modify both plant responses to herbivory by phytophagous insects and insect feeding patterns which, in insect-weed systems, may ultimately determine whether biological control succeeds or fails. The impacts of insect biological control agents on the invasive aquatic weed, water hyacinth (Eichhornia crassipes) vary with nutrient levels in the environment. It was therefore considered important to evaluate nutrient-specific responses of E. crassipes to a grasshopper herbivore, Cornops aquaticum, prior to its release in South Africa. Both plant productivity and the response of E. crassipes to herbivory by C. aquaticum were nutrient dependent. Increases in plant biomass and leaf and ramet production were correlated with increases in nutrients in the water and plant biomass accumulation was reduced by herbivory at all three nutrient levels tested (high = 67%; medium = 100%; low = 400%). C. aquaticum nymphs fed E. crassipes leaves with the lowest nitrogen levels produced the highest biomass of frass during their development, indicating compensatory consumption. The results suggest that environmental nutrient availability will influence efficacy of C. aquaticum. They also provide further evidence that E. crassipes problems are exacerbated by an over-abundance of nutrients in aquatic environments, and that biological control would be a highly effective management tool if aquatic systems in South Africa were less polluted. Additionally, the results also show how an understanding of the fundamental responses of E. crassipes and its insect biocontrol agents to their environment can assist in determining specific management strategies or interventions according to prevailing site-specific conditions.
- Full Text:
- Date Issued: 2013
- Authors: Bownes, Angela , Hill, Martin P , Byrne, Marcus J
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406131 , vital:70241 , xlink:href="https://doi.org/10.1016/j.biocontrol.2013.07.023"
- Description: Environmental nutrient availability can drive and modify both plant responses to herbivory by phytophagous insects and insect feeding patterns which, in insect-weed systems, may ultimately determine whether biological control succeeds or fails. The impacts of insect biological control agents on the invasive aquatic weed, water hyacinth (Eichhornia crassipes) vary with nutrient levels in the environment. It was therefore considered important to evaluate nutrient-specific responses of E. crassipes to a grasshopper herbivore, Cornops aquaticum, prior to its release in South Africa. Both plant productivity and the response of E. crassipes to herbivory by C. aquaticum were nutrient dependent. Increases in plant biomass and leaf and ramet production were correlated with increases in nutrients in the water and plant biomass accumulation was reduced by herbivory at all three nutrient levels tested (high = 67%; medium = 100%; low = 400%). C. aquaticum nymphs fed E. crassipes leaves with the lowest nitrogen levels produced the highest biomass of frass during their development, indicating compensatory consumption. The results suggest that environmental nutrient availability will influence efficacy of C. aquaticum. They also provide further evidence that E. crassipes problems are exacerbated by an over-abundance of nutrients in aquatic environments, and that biological control would be a highly effective management tool if aquatic systems in South Africa were less polluted. Additionally, the results also show how an understanding of the fundamental responses of E. crassipes and its insect biocontrol agents to their environment can assist in determining specific management strategies or interventions according to prevailing site-specific conditions.
- Full Text:
- Date Issued: 2013
Experiences of final year nursing students at a public college of nursing in the Eastern Cape province regarding their preparedness to become registered nurses
- Authors: Mampunge, Fezeka
- Date: 2013
- Subjects: Nursing students -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nurses -- In-service training -- South Africa -- Eastern Cape , Nursing -- Ability testing -- South Africa -- Eastern Cape , Nursing assessment -- South Africa -- Eastern Cape , Nursing -- Examinations , Nursing -- Standards -- South Africa -- Eastern Cape , Nursing student -- Registered nurse -- Experience , Preparedness -- College -- Clinical staff and Clinical accompaniment
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11905 , http://hdl.handle.net/10353/d1006815 , Nursing students -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nurses -- In-service training -- South Africa -- Eastern Cape , Nursing -- Ability testing -- South Africa -- Eastern Cape , Nursing assessment -- South Africa -- Eastern Cape , Nursing -- Examinations , Nursing -- Standards -- South Africa -- Eastern Cape , Nursing student -- Registered nurse -- Experience , Preparedness -- College -- Clinical staff and Clinical accompaniment
- Description: Within the nursing profession, the transition from the student to a graduate nurse is a common rite of passage that marks the end of initial educational preparation in the discipline and the beginning of a professional journey as a nurse and a member of the multidisciplinary team (Nash, Lemcke & Sacre. 2009:48). This is a period of adjustment, stress, growth and development and the transitional nurse is likely to feel uncomfortable, fearful and may experience feelings of inadequacy. This study was undertaken to explore and describe the experiences of final year nursing students at a public college of nursing in the Eastern Cape regarding their preparedness to become registered nurses, with the aim to identify gaps and make recommendations on strategies to close the gaps. The objectives were to explore and describe the experiences of final year nursing students at the public college of nursing in the Eastern Cape Province with regard to their preparedness to become registered nurses and to recommend strategies to improve the preparation of nursing students for transition to become registered nurses. To answer the research question “What are the experiences of final year nursing students at a public college of nursing in the Eastern Cape Province with regard their preparedness to become registered nurses”, a qualitative, explorative and descriptive design was used as a framework for the study. Data were collected by means of unstructured focus group interviews with a purposively selected sample of 27 final year nursing students at the particular college of nursing. Data were analysed using Tesch’s method of analysis for qualitative research. Two themes emerged, revealing that participants at the college experienced preparedness and lack of preparedness to assume the role of a professional nurse. This related to certain aspects that had an impact on the preparation of the final year nursing students for practice and included: curriculum-related aspects; clinical teaching and learning support; learning opportunities; interpersonal relationships between lecturers, students and clinical staff; equipment; and library resources. It was concluded that the learning needs of the nursing students were not adequately catered for, leading to lack of preparedness. Through the involvement of nursing students in the evaluation of their learning, shortfalls in both education and practice areas could therefore be detected. Recommendations regarding strategies to be used to promote preparedness of final year nursing students included: continuous feedback on student performance in the form of exit evaluations on the part of students to identify learning needs; writing of progress reports on the part of clinical practice; and the employment of clinical preceptors with clear role specifications between the lecturers, ward sisters and preceptors to avoid role confusion.
- Full Text:
- Date Issued: 2013
- Authors: Mampunge, Fezeka
- Date: 2013
- Subjects: Nursing students -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nurses -- In-service training -- South Africa -- Eastern Cape , Nursing -- Ability testing -- South Africa -- Eastern Cape , Nursing assessment -- South Africa -- Eastern Cape , Nursing -- Examinations , Nursing -- Standards -- South Africa -- Eastern Cape , Nursing student -- Registered nurse -- Experience , Preparedness -- College -- Clinical staff and Clinical accompaniment
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11905 , http://hdl.handle.net/10353/d1006815 , Nursing students -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nurses -- In-service training -- South Africa -- Eastern Cape , Nursing -- Ability testing -- South Africa -- Eastern Cape , Nursing assessment -- South Africa -- Eastern Cape , Nursing -- Examinations , Nursing -- Standards -- South Africa -- Eastern Cape , Nursing student -- Registered nurse -- Experience , Preparedness -- College -- Clinical staff and Clinical accompaniment
- Description: Within the nursing profession, the transition from the student to a graduate nurse is a common rite of passage that marks the end of initial educational preparation in the discipline and the beginning of a professional journey as a nurse and a member of the multidisciplinary team (Nash, Lemcke & Sacre. 2009:48). This is a period of adjustment, stress, growth and development and the transitional nurse is likely to feel uncomfortable, fearful and may experience feelings of inadequacy. This study was undertaken to explore and describe the experiences of final year nursing students at a public college of nursing in the Eastern Cape regarding their preparedness to become registered nurses, with the aim to identify gaps and make recommendations on strategies to close the gaps. The objectives were to explore and describe the experiences of final year nursing students at the public college of nursing in the Eastern Cape Province with regard to their preparedness to become registered nurses and to recommend strategies to improve the preparation of nursing students for transition to become registered nurses. To answer the research question “What are the experiences of final year nursing students at a public college of nursing in the Eastern Cape Province with regard their preparedness to become registered nurses”, a qualitative, explorative and descriptive design was used as a framework for the study. Data were collected by means of unstructured focus group interviews with a purposively selected sample of 27 final year nursing students at the particular college of nursing. Data were analysed using Tesch’s method of analysis for qualitative research. Two themes emerged, revealing that participants at the college experienced preparedness and lack of preparedness to assume the role of a professional nurse. This related to certain aspects that had an impact on the preparation of the final year nursing students for practice and included: curriculum-related aspects; clinical teaching and learning support; learning opportunities; interpersonal relationships between lecturers, students and clinical staff; equipment; and library resources. It was concluded that the learning needs of the nursing students were not adequately catered for, leading to lack of preparedness. Through the involvement of nursing students in the evaluation of their learning, shortfalls in both education and practice areas could therefore be detected. Recommendations regarding strategies to be used to promote preparedness of final year nursing students included: continuous feedback on student performance in the form of exit evaluations on the part of students to identify learning needs; writing of progress reports on the part of clinical practice; and the employment of clinical preceptors with clear role specifications between the lecturers, ward sisters and preceptors to avoid role confusion.
- Full Text:
- Date Issued: 2013
Identifying drivers of corporate social responsibility for community involvement
- Authors: Gwama, Mzwandile Sebastian
- Date: 2013
- Subjects: Social responsibility of business , Corporate governance
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/6597 , vital:21124
- Description: Organisations operate under unpredictable business environments. These business environments can be classified into internal and external environments. The decision taken by organisations to allocate resources for CSR depends on business environments. Organisations have no control of external business environments. Global financial crisis is an example of an external business environment of which organisations have no control over. The event in the business environments can influence the organisation to review its CSR operations. The beneficiaries of the organisation's CSR program get affected by such decision reviews and face even bigger challenges.
- Full Text:
- Date Issued: 2013
- Authors: Gwama, Mzwandile Sebastian
- Date: 2013
- Subjects: Social responsibility of business , Corporate governance
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/6597 , vital:21124
- Description: Organisations operate under unpredictable business environments. These business environments can be classified into internal and external environments. The decision taken by organisations to allocate resources for CSR depends on business environments. Organisations have no control of external business environments. Global financial crisis is an example of an external business environment of which organisations have no control over. The event in the business environments can influence the organisation to review its CSR operations. The beneficiaries of the organisation's CSR program get affected by such decision reviews and face even bigger challenges.
- Full Text:
- Date Issued: 2013
Development and validation of in-process control test kits for biodiesel production
- Authors: Fibi, Pumza Oscarine
- Date: 2013
- Subjects: Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10416 , http://hdl.handle.net/10948/d1012645 , Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Description: The production of biodiesel from vegetable oils is not a new technology; it has been around since the 1950’s and both the research in terms of the different feedstock that can be used and the production of biodiesel has since been gaining momentum as there needs to be a new, sustainable and domestic alternative to petroleum fuels. These petroleum fuels pose enormous threats to the environment and therefore need to be replaced as they are mostly contributing to climate change and global warming not to mention the frequent price hikes which are crippling the South African economy. Biodiesel production using vegetable oils seems to be and is the future and a law has recently been passed which sanctions the production of biofuel locally.[1] South African fuel producers will instigate obligatory blending of fossil fuel with biofuel as the country moves to encourage investment in its biofuels sector. The production of biodiesel locally and the blending of biodiesel with other petroleum products will reduce the country’s dependence on imported fuel. The already established petrochemical companies like BP, Sasol and Engine are therefore mandated to purchase these biofuels if and when the biofuels meet the required South African National Standard (SANS) 1935 requirements. This is then where the challenge comes as most of these growing biofuel companies cannot afford to purchase testing equipment.The growing companiesthen discover upon completion of the biofuel manufacturing process that their product does not meet the required standard specification. The failure translates to a financial loss as the final product can possibly not be reworked. The aim of the project is then to assist these companies who are manufacturing biofuel, by providing them with in-house biofuel process methods which will allow for early detection, should there be a need to redo a step in the process and not wait until the completion of the production process. These in-house process-testing methods will range from pH determination, titration tests which will determine the soap content and the percentage free fatty acid content, water determination, density and visual testing. It is not cost-effective for these biodiesel manufacturers to send their samples for outsource testing as evidently the results obtained would be out of specification hence the need to provide these biodiesel manufacturers with in-house analytical testing techniques that will aid in monitoring of the biodiesel production.
- Full Text:
- Date Issued: 2013
- Authors: Fibi, Pumza Oscarine
- Date: 2013
- Subjects: Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10416 , http://hdl.handle.net/10948/d1012645 , Biodiesel fuels -- South Africa , Biomass energy -- South Africa
- Description: The production of biodiesel from vegetable oils is not a new technology; it has been around since the 1950’s and both the research in terms of the different feedstock that can be used and the production of biodiesel has since been gaining momentum as there needs to be a new, sustainable and domestic alternative to petroleum fuels. These petroleum fuels pose enormous threats to the environment and therefore need to be replaced as they are mostly contributing to climate change and global warming not to mention the frequent price hikes which are crippling the South African economy. Biodiesel production using vegetable oils seems to be and is the future and a law has recently been passed which sanctions the production of biofuel locally.[1] South African fuel producers will instigate obligatory blending of fossil fuel with biofuel as the country moves to encourage investment in its biofuels sector. The production of biodiesel locally and the blending of biodiesel with other petroleum products will reduce the country’s dependence on imported fuel. The already established petrochemical companies like BP, Sasol and Engine are therefore mandated to purchase these biofuels if and when the biofuels meet the required South African National Standard (SANS) 1935 requirements. This is then where the challenge comes as most of these growing biofuel companies cannot afford to purchase testing equipment.The growing companiesthen discover upon completion of the biofuel manufacturing process that their product does not meet the required standard specification. The failure translates to a financial loss as the final product can possibly not be reworked. The aim of the project is then to assist these companies who are manufacturing biofuel, by providing them with in-house biofuel process methods which will allow for early detection, should there be a need to redo a step in the process and not wait until the completion of the production process. These in-house process-testing methods will range from pH determination, titration tests which will determine the soap content and the percentage free fatty acid content, water determination, density and visual testing. It is not cost-effective for these biodiesel manufacturers to send their samples for outsource testing as evidently the results obtained would be out of specification hence the need to provide these biodiesel manufacturers with in-house analytical testing techniques that will aid in monitoring of the biodiesel production.
- Full Text:
- Date Issued: 2013
RU Open Access Week 21-27 October 2013
- Rhodes University Library, Martindale, Debbie
- Authors: Rhodes University Library , Martindale, Debbie
- Date: 2013
- Language: English
- Type: text
- Identifier: vital:7954 , http://hdl.handle.net/10962/d1006841
- Description: Paper presented at the Rhodes University Open Access event, 24 October 2013. During this event Rhodes University joined the list of signatories to the Berlin Declaration on Open Access to Knowledge. Dr Peter Clayton, DVC Research, signed the Declaration during the Open Access seminar. Other speakers included Ms Natalia Timiraos (BioMed Central) and Ms Elsabe Olivier (former Open Scholarship Manager, UP).
- Full Text:
- Date Issued: 2013
- Authors: Rhodes University Library , Martindale, Debbie
- Date: 2013
- Language: English
- Type: text
- Identifier: vital:7954 , http://hdl.handle.net/10962/d1006841
- Description: Paper presented at the Rhodes University Open Access event, 24 October 2013. During this event Rhodes University joined the list of signatories to the Berlin Declaration on Open Access to Knowledge. Dr Peter Clayton, DVC Research, signed the Declaration during the Open Access seminar. Other speakers included Ms Natalia Timiraos (BioMed Central) and Ms Elsabe Olivier (former Open Scholarship Manager, UP).
- Full Text:
- Date Issued: 2013
The use of tax incentive measure in conjunction with carbon taxes to reduce greenhouse gas emissions and achieve economic growth: a comparative study with lessons for South Africa
- Authors: Poole, Richard
- Date: 2013
- Subjects: Elasticity (Economics) , Substitution (Economics) , Carbon taxes , Carbon taxes -- South Africa , Greenhouse gas mitigation , Greenhouse gas mitigation--South Africa , United Nations Framework Convention on Climate Change -- (1992). Protocols, etc. -- 1997 Dec. 11 , Kyoto Protocol , Substitution elasticity
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:875 , http://hdl.handle.net/10962/d1001607 , Elasticity (Economics) , Substitution (Economics) , Carbon taxes , Carbon taxes -- South Africa , Greenhouse gas mitigation , Greenhouse gas mitigation--South Africa , United Nations Framework Convention on Climate Change -- (1992). Protocols, etc. -- 1997 Dec. 11
- Description: In 1997 industrialized nations, the Third Conference of the Parties to the United Nations Framework Convention on Climate Change, met in Kyoto, Japan to sign a treaty (the “Kyoto Protocol”) in terms of which industrialized nations would be required to reduce their greenhouse gas emission by at least five percent below 1990 levels by the end of the “first commitment period” 2008-2012. South Africa is not regarded as an industrialized nation, but nonetheless acceded to the Kyoto Protocol in 2002. The literature reviewed in the present research reveals that, although idealistic, the Kyoto Protocol has been problematic. Fourteen meetings of the Conference of Parties to the Kyoto Protocol between 1997 and 2011 have achieved little more than to repeatedly defer and redefine Kyoto obligations. This research was undertaken to document the existing environmental taxation policies employed in selected international jurisdictions with a view to providing a framework for environmental tax policy formation in South Africa to assist this country in meeting its “greenhouse gas” emission targets, while at the same time promoting economic growth. A doctrinal research methodology was adopted in this study as it mainly analysed and interpreted legislation and policy documents and therefore the approach was qualitative in nature. An extensive literature survey was performed to document the various environmental policies that have been legislated in the selected jurisdictions. Comparisons were drawn with proposed tax policy measures for South Africa. The literature indicates that in the selected international jurisdictions carbon taxes achieved less-than-optimal results, largely due to political and industry-competitive agendas. With South Africa planning to introduce a carbon tax, it is submitted that the implementation of a carbon tax regime in isolation will be counter-productive, given South Africa’s economic profile. On the basis of the literature reviewed, it was concluded that South Africa should consider “recycling” carbon tax revenues within the economy to fund a broad-based tax incentive regime that will stimulate the change to non-carbon energy whilst promoting growth through sustainable development
- Full Text:
- Date Issued: 2013
- Authors: Poole, Richard
- Date: 2013
- Subjects: Elasticity (Economics) , Substitution (Economics) , Carbon taxes , Carbon taxes -- South Africa , Greenhouse gas mitigation , Greenhouse gas mitigation--South Africa , United Nations Framework Convention on Climate Change -- (1992). Protocols, etc. -- 1997 Dec. 11 , Kyoto Protocol , Substitution elasticity
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:875 , http://hdl.handle.net/10962/d1001607 , Elasticity (Economics) , Substitution (Economics) , Carbon taxes , Carbon taxes -- South Africa , Greenhouse gas mitigation , Greenhouse gas mitigation--South Africa , United Nations Framework Convention on Climate Change -- (1992). Protocols, etc. -- 1997 Dec. 11
- Description: In 1997 industrialized nations, the Third Conference of the Parties to the United Nations Framework Convention on Climate Change, met in Kyoto, Japan to sign a treaty (the “Kyoto Protocol”) in terms of which industrialized nations would be required to reduce their greenhouse gas emission by at least five percent below 1990 levels by the end of the “first commitment period” 2008-2012. South Africa is not regarded as an industrialized nation, but nonetheless acceded to the Kyoto Protocol in 2002. The literature reviewed in the present research reveals that, although idealistic, the Kyoto Protocol has been problematic. Fourteen meetings of the Conference of Parties to the Kyoto Protocol between 1997 and 2011 have achieved little more than to repeatedly defer and redefine Kyoto obligations. This research was undertaken to document the existing environmental taxation policies employed in selected international jurisdictions with a view to providing a framework for environmental tax policy formation in South Africa to assist this country in meeting its “greenhouse gas” emission targets, while at the same time promoting economic growth. A doctrinal research methodology was adopted in this study as it mainly analysed and interpreted legislation and policy documents and therefore the approach was qualitative in nature. An extensive literature survey was performed to document the various environmental policies that have been legislated in the selected jurisdictions. Comparisons were drawn with proposed tax policy measures for South Africa. The literature indicates that in the selected international jurisdictions carbon taxes achieved less-than-optimal results, largely due to political and industry-competitive agendas. With South Africa planning to introduce a carbon tax, it is submitted that the implementation of a carbon tax regime in isolation will be counter-productive, given South Africa’s economic profile. On the basis of the literature reviewed, it was concluded that South Africa should consider “recycling” carbon tax revenues within the economy to fund a broad-based tax incentive regime that will stimulate the change to non-carbon energy whilst promoting growth through sustainable development
- Full Text:
- Date Issued: 2013
Analysis of protein thermostability enhancing factors in industrially important thermus bacteria species
- Kumwenda, Benjamin, Litthauer, Derek, Tastan Bishop, Özlem, Reva, Oleg
- Authors: Kumwenda, Benjamin , Litthauer, Derek , Tastan Bishop, Özlem , Reva, Oleg
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123085 , vital:35404 , https://doi.10.4137/EBO.S12539
- Description: Elucidation of evolutionary factors that enhance protein thermostability is a critical problem and was the focus of this work on Thermus species. Pairs of orthologous sequences of T. scotoductus SA-01 and T. thermophilus HB27, with the largest negative minimum folding energy (MFE) as predicted by the UNAFold algorithm, were statistically analyzed. Favored substitutions of amino acids residues and their properties were determined. Substitutions were analyzed in modeled protein structures to determine their locations and contribution to energy differences using PyMOL and FoldX programs respectively. Dominant trends in amino acid substitutions consistent with differences in thermostability between orthologous sequences were observed. T. thermophilus thermophilic proteins showed an increase in non-polar, tiny, and charged amino acids. An abundance of alanine substituted by serine and threonine, as well as arginine substituted by glutamine and lysine was observed in T. thermophilus HB27. Structural comparison showed that stabilizing mutations occurred on surfaces and loops in protein structures.
- Full Text:
- Date Issued: 2013
- Authors: Kumwenda, Benjamin , Litthauer, Derek , Tastan Bishop, Özlem , Reva, Oleg
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123085 , vital:35404 , https://doi.10.4137/EBO.S12539
- Description: Elucidation of evolutionary factors that enhance protein thermostability is a critical problem and was the focus of this work on Thermus species. Pairs of orthologous sequences of T. scotoductus SA-01 and T. thermophilus HB27, with the largest negative minimum folding energy (MFE) as predicted by the UNAFold algorithm, were statistically analyzed. Favored substitutions of amino acids residues and their properties were determined. Substitutions were analyzed in modeled protein structures to determine their locations and contribution to energy differences using PyMOL and FoldX programs respectively. Dominant trends in amino acid substitutions consistent with differences in thermostability between orthologous sequences were observed. T. thermophilus thermophilic proteins showed an increase in non-polar, tiny, and charged amino acids. An abundance of alanine substituted by serine and threonine, as well as arginine substituted by glutamine and lysine was observed in T. thermophilus HB27. Structural comparison showed that stabilizing mutations occurred on surfaces and loops in protein structures.
- Full Text:
- Date Issued: 2013
Evaluating the need for an Employee Performance Management System in the Eastern Cape Buffalo City Metropolitan Municipality (2012)
- Authors: Mthembu, Bhekisisa Jacob
- Date: 2013
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:11686 , http://hdl.handle.net/10353/d1013579
- Description: Local government is the most important sphere of government, given the fact that, it is the closest to the people. It always experiences the service delivery protest by the community residents it serves. This is often due to unfulfilled promises concerning the delivery of services. Often the annual performance results are also unsatisfactory as compared to the agreed upon service delivery agreements. National treasury as a distributor of funding to Municipalities often has to request the return of the unused funds at the end of the financial year period. This is the results of poor managing and planning for the resources at the disposal of the Municipalities. There has been a number of transformational processes over the past few years in the manner that municipalities are being managed and run on a day today basis. This research is focused on the system enhancement for Buffalo City Metropolitan Municipality which is situated in the Eastern Cape province of South Africa. Buffalo City Metropolitan Municipality, in particular, has in the 2011 Local government election acquired a metropolitan status, which requires another transformationprocess in the manner it is being managed. At the same time this automatically raises the bar on the service delivery mode and expectations by the communities. The Constitution of the Republic of South Africa, act 108 of 1996 states how Municipalities are to render services to the communities. And therefore pieces of egislation serves as a guide for the processes and systems in the municipalities at large. This research examined the need for an employee performance management system in the Buffalo City Metropolitan Municipality for the 2012 period. In the review of the literature, there was a number of analysis of issues such as organisation culture which plays an important role in the system implementation and success. And it became evident that, there can be no success of any system in an organisation without the thorough evaluation of the organisational culture. The data was sourced through close ended questionnaires which were distributed to 51 respondents that were identified. These were five Directors, five General Managers, 10 Programme Managers from across the institution, 30 staff members from across all directorates and one portfolio councillor from Corporate service directorate.Each respondent was given a period of one week to respond to the questionnaires. The research provides suggested solutions to the current problems in Municipalities in terms of employee performance management within the organisation. The recommendations provided are not conclusive as the circumstances cannot be the same from one municipality to the other. In order to ensure their sustainability within Local Government, there is need for an employee performance management system to evaluate the performance of all the employees. The research findings identified the leadership role in ensuring the introduction and successful implementation of the employee performance management system, in order to address the service delivery challenges faced by the municipalities.
- Full Text:
- Date Issued: 2013
- Authors: Mthembu, Bhekisisa Jacob
- Date: 2013
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:11686 , http://hdl.handle.net/10353/d1013579
- Description: Local government is the most important sphere of government, given the fact that, it is the closest to the people. It always experiences the service delivery protest by the community residents it serves. This is often due to unfulfilled promises concerning the delivery of services. Often the annual performance results are also unsatisfactory as compared to the agreed upon service delivery agreements. National treasury as a distributor of funding to Municipalities often has to request the return of the unused funds at the end of the financial year period. This is the results of poor managing and planning for the resources at the disposal of the Municipalities. There has been a number of transformational processes over the past few years in the manner that municipalities are being managed and run on a day today basis. This research is focused on the system enhancement for Buffalo City Metropolitan Municipality which is situated in the Eastern Cape province of South Africa. Buffalo City Metropolitan Municipality, in particular, has in the 2011 Local government election acquired a metropolitan status, which requires another transformationprocess in the manner it is being managed. At the same time this automatically raises the bar on the service delivery mode and expectations by the communities. The Constitution of the Republic of South Africa, act 108 of 1996 states how Municipalities are to render services to the communities. And therefore pieces of egislation serves as a guide for the processes and systems in the municipalities at large. This research examined the need for an employee performance management system in the Buffalo City Metropolitan Municipality for the 2012 period. In the review of the literature, there was a number of analysis of issues such as organisation culture which plays an important role in the system implementation and success. And it became evident that, there can be no success of any system in an organisation without the thorough evaluation of the organisational culture. The data was sourced through close ended questionnaires which were distributed to 51 respondents that were identified. These were five Directors, five General Managers, 10 Programme Managers from across the institution, 30 staff members from across all directorates and one portfolio councillor from Corporate service directorate.Each respondent was given a period of one week to respond to the questionnaires. The research provides suggested solutions to the current problems in Municipalities in terms of employee performance management within the organisation. The recommendations provided are not conclusive as the circumstances cannot be the same from one municipality to the other. In order to ensure their sustainability within Local Government, there is need for an employee performance management system to evaluate the performance of all the employees. The research findings identified the leadership role in ensuring the introduction and successful implementation of the employee performance management system, in order to address the service delivery challenges faced by the municipalities.
- Full Text:
- Date Issued: 2013