Physiological and non-physiological induction of gastrointestinal differentiation
- Authors: Brauns, Seth Clint Aron
- Date: 1999
- Subjects: Gastrointestinal system -- Differentiation , Gastrointestinal system -- Physiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11089 , http://hdl.handle.net/10948/d1015521
- Description: The human colonic carcinoma cell lines HT-29 and Caco-2 both exhibit structural and functional differentiation under appropriate culture conditions. HT-29 can be induced to differentiate by treatment with short-chain fatty acids or acetoacetate. Caco-2 cells differentiate spontaneously upon contact inhibition. In this study HT-29 cells were treated with 5 mM acetate, propionate, butyrate and acetoacetate (physiological inducers) to assess their effects on the expression of carbonic anhydrase 1, sucrase-isomaltase and alkaline phosphatase which are reported to be markers of gastrointestinal differentiation. The maturation induction observed was compared to that of the spontaneous differentiation observed in Caco-2 cells. Assays were performed over an 18 day period. Results showed a close correlation (p < 0.05) between HT-29 and Caco-2 cell on days 4 and 12. These results indicate that differentiation reported in both cell lines is comparable and can be used as a basis for further comparative studies. In addition, parallel experiments to the above were conducted using a selection of nine rationally designed cyclic dipeptides (CDPs) potential drug entities which were chosen as non-physiological inducers. The results showed that the cyclic dipeptides were able to induce the gastrointestinal phenotype as observed in HT-29 cells treated with physiological inducers. Studies on the effects of energy-related metabolism in HT-29 and Caco-2 cells as induced by physiological and non-physiological inducers indicated that energy metabolism is a significant role player in gastrointestinal differentiation. The results reported show a decrease in ATP concentrations indicating that the cyclic dipeptides, like physiological inducers, affect the energy state of the HT-29 cells and thus may effect the differentiation of these cells. A positive correlation was found between histone phsophorylation and differentiation confirming that histone phsophorylation was partly responsible for the decrease in ATP concentrations. It is suggested that the induction of differentiation in HT- 29 cells could be either due to non-specific transcription of genes by activation of a chromatin switch or specific by the activation of signal transduction pathways based on the flux of ATP through the cells. Differential display RT-PCR is probably the most sensitive method that could be used to validate the suggestion of either a nonspecific transcription of genes or a specific differentiation reported for HT-29 cells.
- Full Text:
- Date Issued: 1999
- Authors: Brauns, Seth Clint Aron
- Date: 1999
- Subjects: Gastrointestinal system -- Differentiation , Gastrointestinal system -- Physiology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11089 , http://hdl.handle.net/10948/d1015521
- Description: The human colonic carcinoma cell lines HT-29 and Caco-2 both exhibit structural and functional differentiation under appropriate culture conditions. HT-29 can be induced to differentiate by treatment with short-chain fatty acids or acetoacetate. Caco-2 cells differentiate spontaneously upon contact inhibition. In this study HT-29 cells were treated with 5 mM acetate, propionate, butyrate and acetoacetate (physiological inducers) to assess their effects on the expression of carbonic anhydrase 1, sucrase-isomaltase and alkaline phosphatase which are reported to be markers of gastrointestinal differentiation. The maturation induction observed was compared to that of the spontaneous differentiation observed in Caco-2 cells. Assays were performed over an 18 day period. Results showed a close correlation (p < 0.05) between HT-29 and Caco-2 cell on days 4 and 12. These results indicate that differentiation reported in both cell lines is comparable and can be used as a basis for further comparative studies. In addition, parallel experiments to the above were conducted using a selection of nine rationally designed cyclic dipeptides (CDPs) potential drug entities which were chosen as non-physiological inducers. The results showed that the cyclic dipeptides were able to induce the gastrointestinal phenotype as observed in HT-29 cells treated with physiological inducers. Studies on the effects of energy-related metabolism in HT-29 and Caco-2 cells as induced by physiological and non-physiological inducers indicated that energy metabolism is a significant role player in gastrointestinal differentiation. The results reported show a decrease in ATP concentrations indicating that the cyclic dipeptides, like physiological inducers, affect the energy state of the HT-29 cells and thus may effect the differentiation of these cells. A positive correlation was found between histone phsophorylation and differentiation confirming that histone phsophorylation was partly responsible for the decrease in ATP concentrations. It is suggested that the induction of differentiation in HT- 29 cells could be either due to non-specific transcription of genes by activation of a chromatin switch or specific by the activation of signal transduction pathways based on the flux of ATP through the cells. Differential display RT-PCR is probably the most sensitive method that could be used to validate the suggestion of either a nonspecific transcription of genes or a specific differentiation reported for HT-29 cells.
- Full Text:
- Date Issued: 1999
On using AMOS, EQS, LISREL, Mx, RAMONA and SEPATH for structural equation modeling
- Authors: Peprah, Syvester
- Date: 2000
- Subjects: Latent variables , Multivariate analysis , Mathematical statistics -- computer programs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11082 , http://hdl.handle.net/10948/279 , Latent variables , Multivariate analysis , Mathematical statistics -- computer programs
- Description: Structural Equation Modeling is a common name for the statistical analysis of Structural Equation Models. Structural Equation Models are models that specify relationships between a set of variables and can be specified by means of path diagrams. A number of Structural Equation Modeling programs have been developed. These include, amongst others, AMOS, EQS, LISREL, Mx, RAMONA and SEPATH. A number of studies have been published on the use of some of the applications mentioned above. They include, amongst others, Brown (1986), Waller (1993) and Kano (1997). Structural Equation Models are increasingly being used in the social, economic and behavioral sciences. More and more people are therefore making use of one or more of the Structural Equation Modeling applications on the market. This study is performed with the aim of using each of the Structural Equation Modeling applications AMOS, EQS, LISREL, Mx, RAMONA and SEPATH for the first time and document the experience, joy and the difficulties encountered while using them. This treatise is different from the comparisons already published in that it is based on the use of AMOS, EQS, LISREL, Mx, RAMONA and SEPATH to fit a Structural Equation Model for peer influences on ambition, which is specified for data obtained by Duncan, Haller and Portes (1971), by myself as a first time user of each of the programs mentioned. The impressive features as well as the difficulties encountered are listed for each application. Recommendations for possible improvements to the various applications are also proposed. Finally, recommendations for future studies on the use of Structural Equation Modeling programs are made.
- Full Text:
- Date Issued: 2000
- Authors: Peprah, Syvester
- Date: 2000
- Subjects: Latent variables , Multivariate analysis , Mathematical statistics -- computer programs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11082 , http://hdl.handle.net/10948/279 , Latent variables , Multivariate analysis , Mathematical statistics -- computer programs
- Description: Structural Equation Modeling is a common name for the statistical analysis of Structural Equation Models. Structural Equation Models are models that specify relationships between a set of variables and can be specified by means of path diagrams. A number of Structural Equation Modeling programs have been developed. These include, amongst others, AMOS, EQS, LISREL, Mx, RAMONA and SEPATH. A number of studies have been published on the use of some of the applications mentioned above. They include, amongst others, Brown (1986), Waller (1993) and Kano (1997). Structural Equation Models are increasingly being used in the social, economic and behavioral sciences. More and more people are therefore making use of one or more of the Structural Equation Modeling applications on the market. This study is performed with the aim of using each of the Structural Equation Modeling applications AMOS, EQS, LISREL, Mx, RAMONA and SEPATH for the first time and document the experience, joy and the difficulties encountered while using them. This treatise is different from the comparisons already published in that it is based on the use of AMOS, EQS, LISREL, Mx, RAMONA and SEPATH to fit a Structural Equation Model for peer influences on ambition, which is specified for data obtained by Duncan, Haller and Portes (1971), by myself as a first time user of each of the programs mentioned. The impressive features as well as the difficulties encountered are listed for each application. Recommendations for possible improvements to the various applications are also proposed. Finally, recommendations for future studies on the use of Structural Equation Modeling programs are made.
- Full Text:
- Date Issued: 2000
Ostrich calpastatin purification and partial characterization of the liver inhibitor
- Authors: Roman, Henry James
- Date: 2000
- Subjects: Calpastatin , Protease inhibitors , Ion exchange chromatography , Ostriches
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11090 , http://hdl.handle.net/10948/d1015522
- Description: The isolation and purification of calpastatin from ostrich liver is presented, along with its physicochemical and kinetic properties. By using extraction from liver, ion-exchange chromatography on DEAE-Toyopearl, heating to 90 °C for 10 min and rechromatography on Toyopearl Super-Q 650 S, ostrich calpastatin was isolated and purified from ostrich liver. The purified intact calpastatin showed homogeneity on SDS-PAGE (Mr of 105.6 K). Amino acid analysis showed that ostrich calpastatin resembled that of rabbit liver and human erythrocyte calpastatin. An N-terminal sequence could not be obtained because the N-terminus was found to be blocked by an as yet unknown amino acid residue. The Mr values of degradative forms of ostrich liver calpastatin were determined to be 56 K and 90 K. By using PAG-IEF the pI of the intact form was determined to be 5.1. Ostrich liver calpastatin behaved characteristically like other calpastatins during kinetic analysis. Calpastatin inhibited calpain from pH 6 to 9 and was found to be unaffected by temperatures as high as 100 °C. Calpastatin also inhibited calpain activity at Ca2+ concentrations ranging from 1 to 10 mM. The inhibitor was shown to be phosphorylated because after incubation with alkaline phosphatase there was a decrease in inhibitory activity. No inhibitory effects were detected against other proteases such as chymotrypsin and trypsin, with both proteases inactivating calpastatin completely. Ostrich liver calpain was shown to have a pH optimum of 7.5 and a temperature optimum of 30 °C. In terms of its thermodynamic properties it resembled that of other ostrich proteases; DH, DS and DG being 47.07 kJ/mol, -91.1 J/mol/K and 74.237 kJ/mol, respectively. Ostrich liver calpain showed a Km of 0.14 % (w/v). The enzyme was active at both milli- and micro-molar concentrations of Ca2+. Ostrich liver calpastatin showed many physical, chemical and kinetic properties similar to those of other known calpastatins.
- Full Text: false
- Date Issued: 2000
- Authors: Roman, Henry James
- Date: 2000
- Subjects: Calpastatin , Protease inhibitors , Ion exchange chromatography , Ostriches
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11090 , http://hdl.handle.net/10948/d1015522
- Description: The isolation and purification of calpastatin from ostrich liver is presented, along with its physicochemical and kinetic properties. By using extraction from liver, ion-exchange chromatography on DEAE-Toyopearl, heating to 90 °C for 10 min and rechromatography on Toyopearl Super-Q 650 S, ostrich calpastatin was isolated and purified from ostrich liver. The purified intact calpastatin showed homogeneity on SDS-PAGE (Mr of 105.6 K). Amino acid analysis showed that ostrich calpastatin resembled that of rabbit liver and human erythrocyte calpastatin. An N-terminal sequence could not be obtained because the N-terminus was found to be blocked by an as yet unknown amino acid residue. The Mr values of degradative forms of ostrich liver calpastatin were determined to be 56 K and 90 K. By using PAG-IEF the pI of the intact form was determined to be 5.1. Ostrich liver calpastatin behaved characteristically like other calpastatins during kinetic analysis. Calpastatin inhibited calpain from pH 6 to 9 and was found to be unaffected by temperatures as high as 100 °C. Calpastatin also inhibited calpain activity at Ca2+ concentrations ranging from 1 to 10 mM. The inhibitor was shown to be phosphorylated because after incubation with alkaline phosphatase there was a decrease in inhibitory activity. No inhibitory effects were detected against other proteases such as chymotrypsin and trypsin, with both proteases inactivating calpastatin completely. Ostrich liver calpain was shown to have a pH optimum of 7.5 and a temperature optimum of 30 °C. In terms of its thermodynamic properties it resembled that of other ostrich proteases; DH, DS and DG being 47.07 kJ/mol, -91.1 J/mol/K and 74.237 kJ/mol, respectively. Ostrich liver calpain showed a Km of 0.14 % (w/v). The enzyme was active at both milli- and micro-molar concentrations of Ca2+. Ostrich liver calpastatin showed many physical, chemical and kinetic properties similar to those of other known calpastatins.
- Full Text: false
- Date Issued: 2000
Possible crosstalk between signal transduction pathways in the induction of differentiation in HT-29 cells
- Authors: Jamie, Hajierah
- Date: 2000
- Subjects: Cellular signal transduction , Cell differentiation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11096 , http://hdl.handle.net/10948/d1019684
- Description: The investigation into the mechanisms by which compounds such as butyrate induce differentiation in HT-29 cells, is lacking. The colonic carcinoma cell line, HT-29, undergoes differentiation induction in the presence of butyrate and acetoacetate. The Caco-2 cell line spontaneously differentiates on contact inhibition. In this study, a signal transduction pathway involving ATP, cAMP, Ca2+ and the transcriptional factor CREB was investigated following suggestions that the energy state of the cell and diffferentiation are linked. The activity of the MAP kinase cascade, including possible crosstalk that may exist between these pathways was determined. The HT-29 cells were exposed to 5 mM acetoacetate, butyrate, DMSO and propionate. The results of this differentiation induction were compared to Caco-2 and HeLa cells, which are cervical carcinoma cells. It was found that ATP levels are decreased on differentiation induction in HT-29 cells, which, in turn affected the cAMP concentrations. Theoretically, the inducers do not have any effect on PDE 4 activity, and may facilitate the interaction between cAMP and PKA. Influx of Ca2+ into the cells was inhibited to a degree by the inducers, which was possibly overcome by crosstalk between the cAMP and Ca2+ pathways. CREB activation, lineage-specific gene expression, ERK activity and c-myc expression were all dependent on both the inducers used and the cell-type. PKA played a major role in CREB activation in acetoacetate- and butyrate -induced HT-29, Caco-2 and HeLa cells, while a2+/Calmodulin-dependent kinases I/IV may have a secondary role. Alkaline phosphatase expression in HeLa cells was independent of CREB. Evidence that crosstalk between the MAP kinase cascade and the REBactivation pathways exist, was illustrated by increased CREB activation on ERK inhibition in acetoacetate- and butyrate-induced HT-29 and HeLa cells. Also, the role that ERK played in the cells differed with inducer and cell-type. The dependence of cmyc expression on c-jun and c-fos, appeared to be differentiation induction- and celltype specific. Results from this study indicate the potential use of acetoacetate and butyrate as anti-cancer compounds.
- Full Text:
- Date Issued: 2000
- Authors: Jamie, Hajierah
- Date: 2000
- Subjects: Cellular signal transduction , Cell differentiation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11096 , http://hdl.handle.net/10948/d1019684
- Description: The investigation into the mechanisms by which compounds such as butyrate induce differentiation in HT-29 cells, is lacking. The colonic carcinoma cell line, HT-29, undergoes differentiation induction in the presence of butyrate and acetoacetate. The Caco-2 cell line spontaneously differentiates on contact inhibition. In this study, a signal transduction pathway involving ATP, cAMP, Ca2+ and the transcriptional factor CREB was investigated following suggestions that the energy state of the cell and diffferentiation are linked. The activity of the MAP kinase cascade, including possible crosstalk that may exist between these pathways was determined. The HT-29 cells were exposed to 5 mM acetoacetate, butyrate, DMSO and propionate. The results of this differentiation induction were compared to Caco-2 and HeLa cells, which are cervical carcinoma cells. It was found that ATP levels are decreased on differentiation induction in HT-29 cells, which, in turn affected the cAMP concentrations. Theoretically, the inducers do not have any effect on PDE 4 activity, and may facilitate the interaction between cAMP and PKA. Influx of Ca2+ into the cells was inhibited to a degree by the inducers, which was possibly overcome by crosstalk between the cAMP and Ca2+ pathways. CREB activation, lineage-specific gene expression, ERK activity and c-myc expression were all dependent on both the inducers used and the cell-type. PKA played a major role in CREB activation in acetoacetate- and butyrate -induced HT-29, Caco-2 and HeLa cells, while a2+/Calmodulin-dependent kinases I/IV may have a secondary role. Alkaline phosphatase expression in HeLa cells was independent of CREB. Evidence that crosstalk between the MAP kinase cascade and the REBactivation pathways exist, was illustrated by increased CREB activation on ERK inhibition in acetoacetate- and butyrate-induced HT-29 and HeLa cells. Also, the role that ERK played in the cells differed with inducer and cell-type. The dependence of cmyc expression on c-jun and c-fos, appeared to be differentiation induction- and celltype specific. Results from this study indicate the potential use of acetoacetate and butyrate as anti-cancer compounds.
- Full Text:
- Date Issued: 2000
The analysis of trace gas emissions from landfills
- Authors: Rubidge, Gletwyn Robert
- Date: 2000
- Subjects: Gases, Asphyxiating and poisonnous , Gases -- Analysis , Sanitation -- Environmental engineering
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10983 , http://hdl.handle.net/10948/d1006341 , Gases, Asphyxiating and poisonnous , Gases -- Analysis , Sanitation -- Environmental engineering
- Description: Numerous informal houses have been built on and adjacent to a landfill in iBayi, Port Elizabeth, South Africa, which accepted domestic and industrial waste. Formal housing surrounds most of the site at a greater distance - some 60 m, or further, from the landfill. Both formal and informally housed residents complain of odours, burning eyes, sore throats and headaches - symptoms which they believed were caused by the landfill. The landfill gas and ambient air were analyzed to classify and quantify the VOCs (volatile organic compounds) emitted and then to compare the quantitative data with recognised standards to establish if the residents are at risk. Eighteen target (potentially hazardous) VOCs were quantified. A wide variety of compounds were detected in both the ambient air and landfill gas. The results of the VOC analyses were similar to those of other workers in both the qualitative and quantitative studies. The concentrations of the VOCs were mostly lower than the TLV (threshold limit values) values, but exceeded the MRLs (minimum recommended levels). The combined concentrations of the VOC’s in the ambient air either approached or exceeded the limit values for combined exposure thus indicating that a potential health hazard exists. One third of the VOCs were detected in both the ambient air and the subsurface gas, however, external pollution sources also appear to contribute to the VOC concentrations ambient air. Dangerously high methane concentrations were repeatedly detected in the landfill gas amongst the informal houses. There was a vast improvement in the aesthetic qualities of the landfill since the disposal restriction to accept only domestic refuse and building rubble in July 1997. The ambient air was less odorous and landfill site littered. Fewer informal recyclers were present and their concomitant squabbling over valuables had almost vanished. The management of the iBayi landfill holds much room for improvement. There is potential for serious injury or even death if no action is taken to remedy the problems at the iBayi landfill. A holistic solution will have to be found to make the landfill a safe neighbour. Some complementary analyses (such as pH, heavy metal concentrations in the water and sediments etc.) were performed on the leachate and water surrounding the landfill.
- Full Text:
- Date Issued: 2000
- Authors: Rubidge, Gletwyn Robert
- Date: 2000
- Subjects: Gases, Asphyxiating and poisonnous , Gases -- Analysis , Sanitation -- Environmental engineering
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10983 , http://hdl.handle.net/10948/d1006341 , Gases, Asphyxiating and poisonnous , Gases -- Analysis , Sanitation -- Environmental engineering
- Description: Numerous informal houses have been built on and adjacent to a landfill in iBayi, Port Elizabeth, South Africa, which accepted domestic and industrial waste. Formal housing surrounds most of the site at a greater distance - some 60 m, or further, from the landfill. Both formal and informally housed residents complain of odours, burning eyes, sore throats and headaches - symptoms which they believed were caused by the landfill. The landfill gas and ambient air were analyzed to classify and quantify the VOCs (volatile organic compounds) emitted and then to compare the quantitative data with recognised standards to establish if the residents are at risk. Eighteen target (potentially hazardous) VOCs were quantified. A wide variety of compounds were detected in both the ambient air and landfill gas. The results of the VOC analyses were similar to those of other workers in both the qualitative and quantitative studies. The concentrations of the VOCs were mostly lower than the TLV (threshold limit values) values, but exceeded the MRLs (minimum recommended levels). The combined concentrations of the VOC’s in the ambient air either approached or exceeded the limit values for combined exposure thus indicating that a potential health hazard exists. One third of the VOCs were detected in both the ambient air and the subsurface gas, however, external pollution sources also appear to contribute to the VOC concentrations ambient air. Dangerously high methane concentrations were repeatedly detected in the landfill gas amongst the informal houses. There was a vast improvement in the aesthetic qualities of the landfill since the disposal restriction to accept only domestic refuse and building rubble in July 1997. The ambient air was less odorous and landfill site littered. Fewer informal recyclers were present and their concomitant squabbling over valuables had almost vanished. The management of the iBayi landfill holds much room for improvement. There is potential for serious injury or even death if no action is taken to remedy the problems at the iBayi landfill. A holistic solution will have to be found to make the landfill a safe neighbour. Some complementary analyses (such as pH, heavy metal concentrations in the water and sediments etc.) were performed on the leachate and water surrounding the landfill.
- Full Text:
- Date Issued: 2000
The isolation and partial characterization of a2-antiplasmin and plasminogen from ostrich plasma
- Authors: Thomas, Adele René
- Date: 2000
- Subjects: Serpins , Ostriches , Antifibrinolytic agents , Plasminogen , Plasmin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11080 , http://hdl.handle.net/10948/274 , http://hdl.handle.net/10948/d1005751 , Serpins , Ostriches , Antifibrinolytic agents , Plasminogen , Plasmin
- Description: This study reports the isolation, purification and partial characterisation of the ostrich serpin, a2AP, as well as its target enzyme, ostrich plasmin, in its active and inactive proenzyme, viz. plasminogen, forms. Three different procedures were undertaken to isolate and purify ostrich a2AP. The first one involved L-lysine-Sepharose chromatography, ammonium sulfate fractionation, ion-exchange chromatography on Toyopearl Super-Q 650S, and ostrich plasminogen-Sepharose affinity chromatography. The second procedure replaced the latter chromatographic step with gel filtration on Sephadex G-200 and hydroxylapatite chromatography, while the third one employed instead the theoretically more efficient LBSI-Sepharose chromatographic step. The third procedure yielded purified ostrich a2AP, but the degree of purity and yield were relatively low. Ostrich plasminogen was highly purified after L-lysine-Sepharose chromatography and ostrich plasmin was obtained by the urokinase-activation of the purified ostrich plasminogen Ostrich a2AP revealed an Mr of 77-84 K and two isoelectric forms of pI 3.85 and 6.18. Nterminal sequence analysis showed ostrich a2AP to have only 2 out of 11 residues in common with both those of human and bovine a2AP. Ostrich a2AP showed the largest inhibitory effects on ostrich plasmin, followed by comm. bovine chymotrypsin, trypsin and plasmin, in that order, and it appeared to be a much less potent plasmin inhibitor than bovine aprotinin, but a much more potent one than the synthetic inhibitors, DFP and EACA. Ostrich plasminogen showed an Mr of 92 K and multiple isoelectric forms (~7) in the pI range 6.01-9.18, with a major one of pI 6.01. It showed a total of 775 amino acid residues and its N-terminal sequence showed ~53 percent identity with those of human, rabbit, cat, and ox plasminogens. Ostrich plasmin revealed an Mr of 78 K, two isoelectric forms of pI 4.07 and 6.01, and a total of 638 amino acid residues. N-terminal sequence analysis showed that 2-4 residues are identical to the 5 of human, cat, dog, rabbit, and ox plasmins. The pH and temperature optima of ostrich plasmin were determined as 8.0 and 40 oC, respectively. The thermodynamic and kinetic parameters of ostrich plasmin were computed, and plasmin was shown to prefer Lys to Arg residues in the S1 position. In conclusion, ostrich a2AP, plasminogen and plasmin showed definite similarities to their mammalian counterparts, but there were also significant differences.
- Full Text:
- Date Issued: 2000
- Authors: Thomas, Adele René
- Date: 2000
- Subjects: Serpins , Ostriches , Antifibrinolytic agents , Plasminogen , Plasmin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11080 , http://hdl.handle.net/10948/274 , http://hdl.handle.net/10948/d1005751 , Serpins , Ostriches , Antifibrinolytic agents , Plasminogen , Plasmin
- Description: This study reports the isolation, purification and partial characterisation of the ostrich serpin, a2AP, as well as its target enzyme, ostrich plasmin, in its active and inactive proenzyme, viz. plasminogen, forms. Three different procedures were undertaken to isolate and purify ostrich a2AP. The first one involved L-lysine-Sepharose chromatography, ammonium sulfate fractionation, ion-exchange chromatography on Toyopearl Super-Q 650S, and ostrich plasminogen-Sepharose affinity chromatography. The second procedure replaced the latter chromatographic step with gel filtration on Sephadex G-200 and hydroxylapatite chromatography, while the third one employed instead the theoretically more efficient LBSI-Sepharose chromatographic step. The third procedure yielded purified ostrich a2AP, but the degree of purity and yield were relatively low. Ostrich plasminogen was highly purified after L-lysine-Sepharose chromatography and ostrich plasmin was obtained by the urokinase-activation of the purified ostrich plasminogen Ostrich a2AP revealed an Mr of 77-84 K and two isoelectric forms of pI 3.85 and 6.18. Nterminal sequence analysis showed ostrich a2AP to have only 2 out of 11 residues in common with both those of human and bovine a2AP. Ostrich a2AP showed the largest inhibitory effects on ostrich plasmin, followed by comm. bovine chymotrypsin, trypsin and plasmin, in that order, and it appeared to be a much less potent plasmin inhibitor than bovine aprotinin, but a much more potent one than the synthetic inhibitors, DFP and EACA. Ostrich plasminogen showed an Mr of 92 K and multiple isoelectric forms (~7) in the pI range 6.01-9.18, with a major one of pI 6.01. It showed a total of 775 amino acid residues and its N-terminal sequence showed ~53 percent identity with those of human, rabbit, cat, and ox plasminogens. Ostrich plasmin revealed an Mr of 78 K, two isoelectric forms of pI 4.07 and 6.01, and a total of 638 amino acid residues. N-terminal sequence analysis showed that 2-4 residues are identical to the 5 of human, cat, dog, rabbit, and ox plasmins. The pH and temperature optima of ostrich plasmin were determined as 8.0 and 40 oC, respectively. The thermodynamic and kinetic parameters of ostrich plasmin were computed, and plasmin was shown to prefer Lys to Arg residues in the S1 position. In conclusion, ostrich a2AP, plasminogen and plasmin showed definite similarities to their mammalian counterparts, but there were also significant differences.
- Full Text:
- Date Issued: 2000
Strategies for the improvement of the industrial oxidation of cymene
- Authors: Harmse, Nigel
- Date: 2001
- Subjects: Oxidation , Cymene
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10958 , http://hdl.handle.net/10948/73 , Oxidation , Cymene
- Description: The oxidation of cymene with dioxygen has been investigated in some detail with the view of establishing the feasibility of improving the efficiency of the oxidation process. Of particular interest were the rate of cymene oxidation and the selectivity of the oxidation process for the tertiary cymene hydroperoxide, especially at conversions above 15%. In order to be able to evaluate the selectivity of oxidation processes, a reliable method for analysis of the individual hydroperoxides had to be established. Two methods were investigated, namely reduction of the hydroperoxides to alcohols using ferrous sulphate and reduction using triphenylphosphine, and analysing the reduction products by gas chromatography. Of these two methods, the triphenylphosphine method proved to be superior to the ferrous sulphate method and was used as the method of choice for this investigation. A number of oxidation systems were evaluated in an initial screening experiment for the oxidation of p-cymene. The results of this screening experiment showed that three-phase oxidation systems, i.e. systems containing an organic phase, an aqueous phase and gas, gave significantly lower activities than two-phase oxidation systems. In addition, the use of a base in the aqueous layer does not improve the overall selectivity of the oxidation process, but improves the selectivity towards the tertiary hydroperoxide to some extent due to the decomposition and extraction of primary hydroperoxide into the basic aqueous phase. Oxidation systems using a non-autoxidation catalyst, i.e. a catalyst that does not catalyse the conventional autoxidation of organic compounds, gave by far the most promising results. These systems gave both a high selectivity as well as high reaction rate. From the initial screening experiment, and using multi-factorial statistical techniques, two catalyst systems were selected for investigation, namely vanadium phosphate and boron phosphate. The results of these investigations showed that these two catalysts are remarkably active and selective for the oxidation of p-cymene, giving the cymene tertiary hydroperoxide in selectivities exceeding 85% and at substrate conversions as high as 25%. These results are a considerable improvement over currently known oxidation systems and may offer opportunities for further commercial exploitation.
- Full Text:
- Date Issued: 2001
- Authors: Harmse, Nigel
- Date: 2001
- Subjects: Oxidation , Cymene
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10958 , http://hdl.handle.net/10948/73 , Oxidation , Cymene
- Description: The oxidation of cymene with dioxygen has been investigated in some detail with the view of establishing the feasibility of improving the efficiency of the oxidation process. Of particular interest were the rate of cymene oxidation and the selectivity of the oxidation process for the tertiary cymene hydroperoxide, especially at conversions above 15%. In order to be able to evaluate the selectivity of oxidation processes, a reliable method for analysis of the individual hydroperoxides had to be established. Two methods were investigated, namely reduction of the hydroperoxides to alcohols using ferrous sulphate and reduction using triphenylphosphine, and analysing the reduction products by gas chromatography. Of these two methods, the triphenylphosphine method proved to be superior to the ferrous sulphate method and was used as the method of choice for this investigation. A number of oxidation systems were evaluated in an initial screening experiment for the oxidation of p-cymene. The results of this screening experiment showed that three-phase oxidation systems, i.e. systems containing an organic phase, an aqueous phase and gas, gave significantly lower activities than two-phase oxidation systems. In addition, the use of a base in the aqueous layer does not improve the overall selectivity of the oxidation process, but improves the selectivity towards the tertiary hydroperoxide to some extent due to the decomposition and extraction of primary hydroperoxide into the basic aqueous phase. Oxidation systems using a non-autoxidation catalyst, i.e. a catalyst that does not catalyse the conventional autoxidation of organic compounds, gave by far the most promising results. These systems gave both a high selectivity as well as high reaction rate. From the initial screening experiment, and using multi-factorial statistical techniques, two catalyst systems were selected for investigation, namely vanadium phosphate and boron phosphate. The results of these investigations showed that these two catalysts are remarkably active and selective for the oxidation of p-cymene, giving the cymene tertiary hydroperoxide in selectivities exceeding 85% and at substrate conversions as high as 25%. These results are a considerable improvement over currently known oxidation systems and may offer opportunities for further commercial exploitation.
- Full Text:
- Date Issued: 2001
Studies on the kallikrein-kininogen system of the ostrich (Struthio camelus)
- Authors: Bothma, Leonard Frederick
- Date: 2001
- Subjects: Kallikrein , Kinins , Ostriches
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11067 , http://hdl.handle.net/10948/275 , Kallikrein , Kinins , Ostriches
- Description: Ostrich organs/tissue/fluids were screened for plasma kallikrein-like, tissue kallikrein-like and tonin-like activity in a continuous-fluorogenic-assay system using Pro-Phe-Arg-7-amino-4-methylcoumarine, Phe- Arg-7-amino-4-methylcoumarine and Val-Leu-Arg--7-amino-4-trifluoro-methylcoumarine as substrates. Ostrich liver and kidney showed the highest specific plasma kallikrein-like activity. Ostrich adrenal glands and kidney showed the highest specific tissue kallikrein-like and tonin-like activity. Ostrich high molecular weight kininogen was purified from plasma and low molecular weight kininogen was partially purified. The N-terminal amino acid sequences of both high- and low molecular weight kininogens from ostrich plasma were determined. Ostrich plasma high molecular weight kininogen was purified as a 118 kD protein. The purified high molecular weight kininogen inhibits the cysteine proteinase papain at a ratio of one molecule HKG to two molecules of papain. Ornitho kinin-like molecules were detected in ostrich urine using reverse phase HPLC.
- Full Text:
- Date Issued: 2001
- Authors: Bothma, Leonard Frederick
- Date: 2001
- Subjects: Kallikrein , Kinins , Ostriches
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11067 , http://hdl.handle.net/10948/275 , Kallikrein , Kinins , Ostriches
- Description: Ostrich organs/tissue/fluids were screened for plasma kallikrein-like, tissue kallikrein-like and tonin-like activity in a continuous-fluorogenic-assay system using Pro-Phe-Arg-7-amino-4-methylcoumarine, Phe- Arg-7-amino-4-methylcoumarine and Val-Leu-Arg--7-amino-4-trifluoro-methylcoumarine as substrates. Ostrich liver and kidney showed the highest specific plasma kallikrein-like activity. Ostrich adrenal glands and kidney showed the highest specific tissue kallikrein-like and tonin-like activity. Ostrich high molecular weight kininogen was purified from plasma and low molecular weight kininogen was partially purified. The N-terminal amino acid sequences of both high- and low molecular weight kininogens from ostrich plasma were determined. Ostrich plasma high molecular weight kininogen was purified as a 118 kD protein. The purified high molecular weight kininogen inhibits the cysteine proteinase papain at a ratio of one molecule HKG to two molecules of papain. Ornitho kinin-like molecules were detected in ostrich urine using reverse phase HPLC.
- Full Text:
- Date Issued: 2001
The role of Venda culture in nature conservation : a case study of the inhabitants of the Tshivhase area
- Khorommbi, Konanani Christopher
- Authors: Khorommbi, Konanani Christopher
- Date: 2001
- Subjects: Nature conservation -- South Africa -- Venda , Venda (South Africa)
- Language: English
- Type: Thesis , Masters , MTech (Nature Conservation)
- Identifier: vital:10975 , http://hdl.handle.net/10948/d1002119 , Nature conservation -- South Africa -- Venda , Venda (South Africa)
- Description: This thesis investigates the role of Venda culture in nature conservation and natural resource management. The project is based on the premise that the survival of human beings is entirely dependent on the extent to which different cultures conserve the natural environment. The researcher assumes that the scientific approach is not the only one that is capable of addressing environmental issues but that indigenous technology has a positive role to play. The study specifically looks at the relationship between the Venda culture and conservation of land, water, plants and animals. In order to achieve this goal, the study was divided into five chapters. The introductory chapter includes the statement of problem, delimitations of the study, the definitions of concepts, the assumptions, and the significance of the study. The literature review provides an overview of the indigenous strategies of nature conservation and natural resource management from a broader international approach to a specifically Venda approach. The researcher looked at the cultural perspective of the four areas under investigation. This chapter is followed by themethodological justification that discusses the social survey as a researchmethod for carrying out this study. The results presented in the fourth chapter comprise raw data from the three sectors of respondents, namely traditional leaders, traditional healers and villagers. Under each item, common responses have been grouped together for presentation and specific responses for the three sectors were presented independently from one another. This has provided the researcher with the opportunity to identify areas of similarities and differences among the three sectors. Both complementary and conflicting ideas are summarised and used as a basis for analysis. The results reflect a high degree of agreement among respondents. The discussion in chapter five was based on the results and relevant literature. The researcher’s approach was to depict the most common views among the three sectors and their conservation implications. In the discussion, the strengths and weaknesses of the Venda cultural approach were exposed in relation to the five sub-problems under investigation. The recognition of the Venda indigenous technology of natural resource management has been proposed because of its importance in nature conservation. In the recommendations the formulation of policy that overlooks local values was criticised. The use of the Venda approach as a basis for the development of conservation projects was strongly recommended. Lastly, itwas proposed that further investigations of specific aspects of the Venda cultural be considered for possible incorporation in future management of the natural environment.
- Full Text:
- Date Issued: 2001
- Authors: Khorommbi, Konanani Christopher
- Date: 2001
- Subjects: Nature conservation -- South Africa -- Venda , Venda (South Africa)
- Language: English
- Type: Thesis , Masters , MTech (Nature Conservation)
- Identifier: vital:10975 , http://hdl.handle.net/10948/d1002119 , Nature conservation -- South Africa -- Venda , Venda (South Africa)
- Description: This thesis investigates the role of Venda culture in nature conservation and natural resource management. The project is based on the premise that the survival of human beings is entirely dependent on the extent to which different cultures conserve the natural environment. The researcher assumes that the scientific approach is not the only one that is capable of addressing environmental issues but that indigenous technology has a positive role to play. The study specifically looks at the relationship between the Venda culture and conservation of land, water, plants and animals. In order to achieve this goal, the study was divided into five chapters. The introductory chapter includes the statement of problem, delimitations of the study, the definitions of concepts, the assumptions, and the significance of the study. The literature review provides an overview of the indigenous strategies of nature conservation and natural resource management from a broader international approach to a specifically Venda approach. The researcher looked at the cultural perspective of the four areas under investigation. This chapter is followed by themethodological justification that discusses the social survey as a researchmethod for carrying out this study. The results presented in the fourth chapter comprise raw data from the three sectors of respondents, namely traditional leaders, traditional healers and villagers. Under each item, common responses have been grouped together for presentation and specific responses for the three sectors were presented independently from one another. This has provided the researcher with the opportunity to identify areas of similarities and differences among the three sectors. Both complementary and conflicting ideas are summarised and used as a basis for analysis. The results reflect a high degree of agreement among respondents. The discussion in chapter five was based on the results and relevant literature. The researcher’s approach was to depict the most common views among the three sectors and their conservation implications. In the discussion, the strengths and weaknesses of the Venda cultural approach were exposed in relation to the five sub-problems under investigation. The recognition of the Venda indigenous technology of natural resource management has been proposed because of its importance in nature conservation. In the recommendations the formulation of policy that overlooks local values was criticised. The use of the Venda approach as a basis for the development of conservation projects was strongly recommended. Lastly, itwas proposed that further investigations of specific aspects of the Venda cultural be considered for possible incorporation in future management of the natural environment.
- Full Text:
- Date Issued: 2001
An evaluation of family poultry production systems in the Northern region
- Authors: Ranwedzi, Ndivhuho Emmanuel
- Date: 2002
- Subjects: Poultry -- Breeding , Poultry -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10962 , http://hdl.handle.net/10948/93 , Poultry -- Breeding , Poultry -- Feeding and feeds
- Description: Introduction : Family Poultry (FP) are birds of indigenous breeds living in almost symbiotic relationship with human communities. The chickens are usually free ranging, or have very limited restrains on their access to the village environment. African livestock population statistics for 1995 indicates poultry to be the most numerous species of farm animal (Anonymous, 1996a). More than 80% of poultry are kept in rural areas and contribute substantially to annual egg and meat production (Sonaiya, 1997). Throughout Africa poultry production stems from ancient traditional practices. FP is the most important type of poultry kept on the continent. In general, village producers keep small flocks of between 5 and 20 birds per household (Gueye, 1997a). Women and children play a key role in their management (Kitalyi, 1996). Because of its productivity, FP production has been neglected and is frequently considered by farmers as an insignificant occupation compared with other agricultural activities. Nevertheless, outside urban centers and especially in non-coastal areas, FP provides the population with a vital source of protein and income. In addition, they play an important role within the context of many social and/ or religious ceremonies. Although this type of poultry constitutes an important part of food security for rural households in South Africa, scientists have not explored this area of research for improving or recording this production system. Yet, FP survived for decades without being wiped out, in the interim, their keepers have gathered and stored a wealth of knowledge and experience, ensuring the survival of this genetic resource. The objective of this study was to collect the baseline data on FP production systems, determine their production constraints and achieve data for future use in the intervention strategies.
- Full Text:
- Date Issued: 2002
- Authors: Ranwedzi, Ndivhuho Emmanuel
- Date: 2002
- Subjects: Poultry -- Breeding , Poultry -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10962 , http://hdl.handle.net/10948/93 , Poultry -- Breeding , Poultry -- Feeding and feeds
- Description: Introduction : Family Poultry (FP) are birds of indigenous breeds living in almost symbiotic relationship with human communities. The chickens are usually free ranging, or have very limited restrains on their access to the village environment. African livestock population statistics for 1995 indicates poultry to be the most numerous species of farm animal (Anonymous, 1996a). More than 80% of poultry are kept in rural areas and contribute substantially to annual egg and meat production (Sonaiya, 1997). Throughout Africa poultry production stems from ancient traditional practices. FP is the most important type of poultry kept on the continent. In general, village producers keep small flocks of between 5 and 20 birds per household (Gueye, 1997a). Women and children play a key role in their management (Kitalyi, 1996). Because of its productivity, FP production has been neglected and is frequently considered by farmers as an insignificant occupation compared with other agricultural activities. Nevertheless, outside urban centers and especially in non-coastal areas, FP provides the population with a vital source of protein and income. In addition, they play an important role within the context of many social and/ or religious ceremonies. Although this type of poultry constitutes an important part of food security for rural households in South Africa, scientists have not explored this area of research for improving or recording this production system. Yet, FP survived for decades without being wiped out, in the interim, their keepers have gathered and stored a wealth of knowledge and experience, ensuring the survival of this genetic resource. The objective of this study was to collect the baseline data on FP production systems, determine their production constraints and achieve data for future use in the intervention strategies.
- Full Text:
- Date Issued: 2002
An investigation of the effect of time of pruning on the growth and fruiting of lemons [Citrus limon(L.) Burmann f.] cv. Eureka
- Authors: Pittaway, Timothy Michael
- Date: 2002
- Subjects: Lemon , Pruning , Fruit-culture
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10974 , http://hdl.handle.net/10948/106 , Lemon , Pruning , Fruit-culture
- Description: Pruning has been used to reduce tree size, allow light penetration into trees, improve yield, improve fruit size and fruit quality, overcome alternate bearing, assist fruit harvest, and assist pest and disease control. The use of pruning has increased due to improving agricultural management techniques such as high planting densities, use of mechanical machinery in orchards and the need for effective pesticide and pathological chemical spray applications. The main objective of this study was to obtain a practical means of manipulating lemon trees at the right time. Pruning at the correct time to cultivate productive trees that produce quality fruit would have financial benefits. Lemon fruit quality is dependent on market demand and involves a number of features such as fruit shelf life, rind thickness, fruit size, rind colour, and juice content. The study was conducted on ’Eureka’ lemon trees budded on C. volkameriana rootstock, bearing the fifth and sixth commercial crops in 1999 and 2000 respectively. Twelve monthly pruning treatments per year were conducted on one row of trees starting in December 1997 (site 1) and repeated in the second year on the adjacent row of the same orchard starting in December 1998 (site 2). Selective pruning heading cuts were applied below the intercalation on the intercalary units. Potential branch bearing units were tagged and assessed during the harvest and flowering periods. Summer pruning between 16 to 19 months before the subsequent April/May harvest, resulted in the longest and most complex (intercalation sprouted per axil) vegetative response. The estimated crop value indicated that summer pruning treatments produced the highest income. This was ascribed not to differences in fruit size or quality, but to an increase in yield. The industry’s trend is to prune citrus from post-harvest to the pre-bloom stage. Results from this study have provided a beneficial cultural practice to prune during the summer months and provides a practice to optimise farm production and profit margins.
- Full Text:
- Date Issued: 2002
- Authors: Pittaway, Timothy Michael
- Date: 2002
- Subjects: Lemon , Pruning , Fruit-culture
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10974 , http://hdl.handle.net/10948/106 , Lemon , Pruning , Fruit-culture
- Description: Pruning has been used to reduce tree size, allow light penetration into trees, improve yield, improve fruit size and fruit quality, overcome alternate bearing, assist fruit harvest, and assist pest and disease control. The use of pruning has increased due to improving agricultural management techniques such as high planting densities, use of mechanical machinery in orchards and the need for effective pesticide and pathological chemical spray applications. The main objective of this study was to obtain a practical means of manipulating lemon trees at the right time. Pruning at the correct time to cultivate productive trees that produce quality fruit would have financial benefits. Lemon fruit quality is dependent on market demand and involves a number of features such as fruit shelf life, rind thickness, fruit size, rind colour, and juice content. The study was conducted on ’Eureka’ lemon trees budded on C. volkameriana rootstock, bearing the fifth and sixth commercial crops in 1999 and 2000 respectively. Twelve monthly pruning treatments per year were conducted on one row of trees starting in December 1997 (site 1) and repeated in the second year on the adjacent row of the same orchard starting in December 1998 (site 2). Selective pruning heading cuts were applied below the intercalation on the intercalary units. Potential branch bearing units were tagged and assessed during the harvest and flowering periods. Summer pruning between 16 to 19 months before the subsequent April/May harvest, resulted in the longest and most complex (intercalation sprouted per axil) vegetative response. The estimated crop value indicated that summer pruning treatments produced the highest income. This was ascribed not to differences in fruit size or quality, but to an increase in yield. The industry’s trend is to prune citrus from post-harvest to the pre-bloom stage. Results from this study have provided a beneficial cultural practice to prune during the summer months and provides a practice to optimise farm production and profit margins.
- Full Text:
- Date Issued: 2002
Effect of selected physical and production traits on the tick burdens of beef cattle
- Authors: Botha, Theunis Christoffel
- Date: 2002
- Subjects: Beef cattle -- Breeding
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10966 , http://hdl.handle.net/10948/91 , Beef cattle -- Breeding
- Description: The objective of this study was to determine the effect of selected traits such as age, sex, body weight, body length and height, body condition score (BCS), coatscore (CS), skin thickness and average skin surface temperature on tick burdens in beef cattle. Bonsmara cattle (n= 143) were used to measure visible tick counts, body condition score, coat score, skin thickness, body height and length, body weight, body surface temperature, gender and inter calving period. Measurements were taken from April to December. All animals were managed extensively on natural and cultivated pastures near George in the Southern Cape. Female animals had significantly (p<0.05) greater tick infestation (37.98±2.7) compared to male animals (16.52±1.2). Age was a significant factor (p<0.001) with the younger animals below two years having (46.40±5.26) more ticks than those of two years and older (20.15±2.44). A significant negative correlation (p<0.001; -0.29) was reported between the infestation of ticks on the animals and the age of the animal. Animals with an average body weight below 250kg had 42% (p<0.05) more ticks compared to animals with a body weight above 250kg. Age of the animal and weight were highly correlated (r= 0.70); p<0.001), while the correlation between the number of ticks per cow and the mean weight was negatively correlated (r= -0.37; p<0.001). Skin surface temperature significantly influenced tick infestation on the animals (p<0.001). The degree of infestation increased as body surface temperature exceeded 30º C. Coat score, skin thickness, body condition score and inter calving period did not significantly influence tick infestation on the animals. The infestation of ticks on the animals were significantly influenced by body height (p<0.019) and body length (p<0.001). Animals smaller than a 130cm in height had a significantly (p<0.05) greater tick infestation (36.5±5) compared to animals taller than 130cm (21.2±1.5). This trend was also observed for body length. Animals with a body length shorter than 145cm had a greater (p<0.05) average tick infestation of 41.3±4.5 compared to 23.2±1.3 to animals longer than 145cm, indicating a 44% greater tick infestation in favour of the shorter animals. For increased production and tick resistance animals should have smoother coats and be able to dissipate heat effectively.
- Full Text:
- Date Issued: 2002
- Authors: Botha, Theunis Christoffel
- Date: 2002
- Subjects: Beef cattle -- Breeding
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10966 , http://hdl.handle.net/10948/91 , Beef cattle -- Breeding
- Description: The objective of this study was to determine the effect of selected traits such as age, sex, body weight, body length and height, body condition score (BCS), coatscore (CS), skin thickness and average skin surface temperature on tick burdens in beef cattle. Bonsmara cattle (n= 143) were used to measure visible tick counts, body condition score, coat score, skin thickness, body height and length, body weight, body surface temperature, gender and inter calving period. Measurements were taken from April to December. All animals were managed extensively on natural and cultivated pastures near George in the Southern Cape. Female animals had significantly (p<0.05) greater tick infestation (37.98±2.7) compared to male animals (16.52±1.2). Age was a significant factor (p<0.001) with the younger animals below two years having (46.40±5.26) more ticks than those of two years and older (20.15±2.44). A significant negative correlation (p<0.001; -0.29) was reported between the infestation of ticks on the animals and the age of the animal. Animals with an average body weight below 250kg had 42% (p<0.05) more ticks compared to animals with a body weight above 250kg. Age of the animal and weight were highly correlated (r= 0.70); p<0.001), while the correlation between the number of ticks per cow and the mean weight was negatively correlated (r= -0.37; p<0.001). Skin surface temperature significantly influenced tick infestation on the animals (p<0.001). The degree of infestation increased as body surface temperature exceeded 30º C. Coat score, skin thickness, body condition score and inter calving period did not significantly influence tick infestation on the animals. The infestation of ticks on the animals were significantly influenced by body height (p<0.019) and body length (p<0.001). Animals smaller than a 130cm in height had a significantly (p<0.05) greater tick infestation (36.5±5) compared to animals taller than 130cm (21.2±1.5). This trend was also observed for body length. Animals with a body length shorter than 145cm had a greater (p<0.05) average tick infestation of 41.3±4.5 compared to 23.2±1.3 to animals longer than 145cm, indicating a 44% greater tick infestation in favour of the shorter animals. For increased production and tick resistance animals should have smoother coats and be able to dissipate heat effectively.
- Full Text:
- Date Issued: 2002
Evaluation of broiler performance under small-scale and semi-commercial farming conditions in the Northern Province
- Authors: Nembilwi, Daniel
- Date: 2002
- Subjects: Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10964 , http://hdl.handle.net/10948/92 , Broilers (Chickens)
- Description: Introduction: In the Northern region of the Northern Province, there is an increase in the number of small-scale and semi-commercial farms in all sectors of agriculture. This includes the production of grains, vegetables, fruits and livestock. Broiler production seemed to be a much-preferred alternative of development in community based projects and emerging farmers have shown keen interest. This is because broiler production requires less space than ruminants and is ready for human consumption within a very short period of time. Poultry production provides a constant source of income and protein with a big customer demand. By the year 2000, broiler production is expected to reach 4000 million and 7.5 million tons of poultry feeds in the world. Many countries have initiated programs aimed at improving small-scale poultry as a means of helping to bring socio- economic benefits to rural communities (Panda, 1989). In order to feed the ever rising population of 4.9 million in the Northern Province (1996 Census), there must be an increase in the production of broilers as a primary source of top quality animal protein. With the high unemployment rate (46%) in the Northern Province, self- employment projects are needed which comply with agro- processing. More than 90 community projects based on poultry production in the Northern region of the Northern Province had been established in order to reduce the high rate of unemployment. Today more people have engaged themselves in community projects especially in poultry production than ever before. Some of the constrains of broiler production at both small-scale and semi-commercial farming condition includes inadequate ventilation system. Lack of suitable health care facilities and frequently the absence of efficient marketing structures form part of the obstacles to the rapid development of poultry production. Feed costs which remain the highest input cost in the production of broilers represent between 60 to 70% or more of the total costs of broiler productions (Benyi and Habi, 1998). Lack of management skills and training for small and semi-commercial farmers makes it difficult to obtain optimum performance. The objective of this study is to evaluate the performance of broilers as influenced by environmental factors under small.
- Full Text:
- Date Issued: 2002
- Authors: Nembilwi, Daniel
- Date: 2002
- Subjects: Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10964 , http://hdl.handle.net/10948/92 , Broilers (Chickens)
- Description: Introduction: In the Northern region of the Northern Province, there is an increase in the number of small-scale and semi-commercial farms in all sectors of agriculture. This includes the production of grains, vegetables, fruits and livestock. Broiler production seemed to be a much-preferred alternative of development in community based projects and emerging farmers have shown keen interest. This is because broiler production requires less space than ruminants and is ready for human consumption within a very short period of time. Poultry production provides a constant source of income and protein with a big customer demand. By the year 2000, broiler production is expected to reach 4000 million and 7.5 million tons of poultry feeds in the world. Many countries have initiated programs aimed at improving small-scale poultry as a means of helping to bring socio- economic benefits to rural communities (Panda, 1989). In order to feed the ever rising population of 4.9 million in the Northern Province (1996 Census), there must be an increase in the production of broilers as a primary source of top quality animal protein. With the high unemployment rate (46%) in the Northern Province, self- employment projects are needed which comply with agro- processing. More than 90 community projects based on poultry production in the Northern region of the Northern Province had been established in order to reduce the high rate of unemployment. Today more people have engaged themselves in community projects especially in poultry production than ever before. Some of the constrains of broiler production at both small-scale and semi-commercial farming condition includes inadequate ventilation system. Lack of suitable health care facilities and frequently the absence of efficient marketing structures form part of the obstacles to the rapid development of poultry production. Feed costs which remain the highest input cost in the production of broilers represent between 60 to 70% or more of the total costs of broiler productions (Benyi and Habi, 1998). Lack of management skills and training for small and semi-commercial farmers makes it difficult to obtain optimum performance. The objective of this study is to evaluate the performance of broilers as influenced by environmental factors under small.
- Full Text:
- Date Issued: 2002
Evaluation of manufacturing processes for the production of atrazine
- Authors: Schaefer, Melissa Claire
- Date: 2002
- Subjects: Atrazine , Pesticides
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10960 , http://hdl.handle.net/10948/96 , Atrazine , Pesticides
- Description: This report describes the results of investigations carried out with the view to find an alternative for MIBK as solvent for the production of atrazine as currently practised by Dow AgroSciences in South Africa. The main motivating factors for the said investigation was: · to increase the yield of atrazine produced, · to reduce the amount of organics, consisting essentially of reaction solvent containing dissolved product, in the aqueous process effluent, and · to improve the properties of the solid (crystalline) product to enable easier product formulation. Synthetic reactions carried out in the absence of organic solvent, i.e. in essentially a 10% NaCl solution containing a surfactant, proved rather disappointing. Low yields of atrazine were obtained together with relatively large amounts of by-products such as propazine and simazine, irrespective of the nature of the surfactant. The reason for the low yield of atrazine and high yields of by-products were established in competing substitution reactions. In these reactions, IPA was reacted with an equimolar mixture of cyanuric chloride and mono-i (first reaction intermediate) in both aqueous medium and in toluene as reaction solvent. The results of these experiments indicated that in aqueous medium IPA reacts faster with mono-i than cyanuric chloride to give propazine as by-product. In toluene, however, the preferred reaction is with cyanuric chloride to give more mono-i as product. Toluene was investigated as an alternative organic solvent to MIBK in view of its desirable properties such as low solubility in water and ease of recovery and recycling. The synthesis of atrazine was optimised in terms of addition sequence and rates of amine reagents and base (HCl acceptor), both by means of benchscale reactions and reaction calorimetry. Reaction energy profiles indicated that both the reaction of secondary amine/NaOH and primary amine/NaOH were virtually instantaneous. This implies that the reaction can be performed under feed control conditions. Of particular importance in ensuring high yields of high purity product was accurate temperature control (since both reaction steps are highly exothermic) and mixing. The latter was important in view of the rapid reaction of amine/NaOH with cyanuric chloride, as well as the possible reaction of mono-i, the first reaction intermediate, with IPA in cases of local excesses of reagent. Under optimum conditions, a yield of atrazine > 97% could be achieved where the resultant product was well within stipulated product specifications. In view of the results obtained, the following recommendations regarding the synthesis of atrazine in toluene as reaction solvent can be made: · Use a reagent addition sequence that staggers the addition of amine and NaOH in such a manner that amine is added first for a short while, followed by the simultaneous addition of amine and NaOH, and ending with NaOH. Use two reaction vessels in series, one for the IPA addition reaction and one for the MEA addition reaction. In this manner the reaction can be run on a continuous basis since no lag time between amine additions is required. Also, smaller reactors may be used whilst maintaining high production rates. Smaller reactors will improve both temperature control and mixing of reagents.
- Full Text:
- Date Issued: 2002
- Authors: Schaefer, Melissa Claire
- Date: 2002
- Subjects: Atrazine , Pesticides
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10960 , http://hdl.handle.net/10948/96 , Atrazine , Pesticides
- Description: This report describes the results of investigations carried out with the view to find an alternative for MIBK as solvent for the production of atrazine as currently practised by Dow AgroSciences in South Africa. The main motivating factors for the said investigation was: · to increase the yield of atrazine produced, · to reduce the amount of organics, consisting essentially of reaction solvent containing dissolved product, in the aqueous process effluent, and · to improve the properties of the solid (crystalline) product to enable easier product formulation. Synthetic reactions carried out in the absence of organic solvent, i.e. in essentially a 10% NaCl solution containing a surfactant, proved rather disappointing. Low yields of atrazine were obtained together with relatively large amounts of by-products such as propazine and simazine, irrespective of the nature of the surfactant. The reason for the low yield of atrazine and high yields of by-products were established in competing substitution reactions. In these reactions, IPA was reacted with an equimolar mixture of cyanuric chloride and mono-i (first reaction intermediate) in both aqueous medium and in toluene as reaction solvent. The results of these experiments indicated that in aqueous medium IPA reacts faster with mono-i than cyanuric chloride to give propazine as by-product. In toluene, however, the preferred reaction is with cyanuric chloride to give more mono-i as product. Toluene was investigated as an alternative organic solvent to MIBK in view of its desirable properties such as low solubility in water and ease of recovery and recycling. The synthesis of atrazine was optimised in terms of addition sequence and rates of amine reagents and base (HCl acceptor), both by means of benchscale reactions and reaction calorimetry. Reaction energy profiles indicated that both the reaction of secondary amine/NaOH and primary amine/NaOH were virtually instantaneous. This implies that the reaction can be performed under feed control conditions. Of particular importance in ensuring high yields of high purity product was accurate temperature control (since both reaction steps are highly exothermic) and mixing. The latter was important in view of the rapid reaction of amine/NaOH with cyanuric chloride, as well as the possible reaction of mono-i, the first reaction intermediate, with IPA in cases of local excesses of reagent. Under optimum conditions, a yield of atrazine > 97% could be achieved where the resultant product was well within stipulated product specifications. In view of the results obtained, the following recommendations regarding the synthesis of atrazine in toluene as reaction solvent can be made: · Use a reagent addition sequence that staggers the addition of amine and NaOH in such a manner that amine is added first for a short while, followed by the simultaneous addition of amine and NaOH, and ending with NaOH. Use two reaction vessels in series, one for the IPA addition reaction and one for the MEA addition reaction. In this manner the reaction can be run on a continuous basis since no lag time between amine additions is required. Also, smaller reactors may be used whilst maintaining high production rates. Smaller reactors will improve both temperature control and mixing of reagents.
- Full Text:
- Date Issued: 2002
Modelling the broiler performance under small-scales and semi commercial management condition
- Authors: Prince, Siliga Hulisani
- Date: 2002
- Subjects: Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10963 , http://hdl.handle.net/10948/110 , Broilers (Chickens)
- Description: Introduction : World poultry meat consumption consists of three major segments: broilers, turkeys, and other poultry which includes spent egg layers, spent breeder hens/males, ducks, geese, guinea-fowl, pheasants, quail, ratites, ostriches and emus. Broilers clearly dominate the world poultry consumption contributing about 70 percent to the world poultry market. Turkeys account for about eight percent while other poultry provides the balance of 22 percent (Roenick, 1998). The world broiler industry has been, and continues to be a very dynamic industry. It has been typified with unprecedented growth in terms of production. In 1961, the world produced 6.5 billion broilers. By 1990, the total annual production was a little over 27 billion broilers and by 1996, total world production had grown to 35.3 billion (Van der Sluis., 1999). This represents a fivefold increase in 35 years, and it has not stopped growing since, with world production surpassed 38 billion in 1998 (Van der Sluis., 1999). In Africa the human population was estimated to be 819.9 million, while the total poultry population of the continent was estimated as 1.133 million chickens, 15 million ducks and seven million turkeys in 1998. Production scale being 1.781701 metric tones (MT) of hen eggs, 2.269000 MT of chickens’ meat, 32.824MT of turkey meat and 52.989MT of duck meat (Gueye, 2000). Commercial poultry products are also imported and exported. For example, 12.000 metric tons of poultry meat was estimated to have been exported from South Africa in 1999, whereas 87.000 and 10.000 metric tons of poultry meat was imported into South Africa and Egypt, respectively (Gueye, 2000). In South Africa poultry production is moving toward the 21st century with the potential for increased development being greater than any other sector of the agricultural industry. The small-scale to medium-scale or semi-commercial farmer is becoming a role-player that needs to be serviced by the poultry industry (Fourie, 2000). The industry will have to take up the challenges that are facing an entrepreneur starting with a poultry venture. The poultry industry has developed from a humble beginning at the turn of the century into a multi-million Rand enterprise where protein is provided through the commercial and small marketing sector (Fourie, 2000). Some of the larger commercial companies market three million birds per week. It is estimated that the commercial company sector is responsible for 75 to 85 percent of production and marketing of white meat in this country whereas the small-scale and medium-scale or semi commercial poultry production enterprises provide 20 to 25 percent of the white meat through the sales of slaughtered and live poultry sale to rural, peri-urban and urban settlements (Fourie., 2000). Small-scale and semi-commercial broiler production is developed by entrepreneurs who buy day-old chicks from the big commercial hatcheries, rear them to the age of five to eight weeks and sell them through a marketing network or small outlets. The small-scale consist of farmers who reared 100 t0 1000 chickens per batch and semi-commercial consist of farmers who reared 1000 to 5000 chickens per batch. These types of farmers acquire very little technical backup in terms of a training and services center where assistance is available every day of the week. The broiler industry requires birds that are of commercial crosses selected for their ability to grow very fast and produce a good quality carcass in the shortest time frame. The small farmers purchase broiler stock, as the day-old chicks from commercial hatcheries and reared them up to six to eight weeks before slaughtered or sold as live chickens. The selling of live chickens through a network outlets system is becoming an industry that is growing at a steady pace, as household food security is becoming important to communities (Fourie, 2000). The price of feed is the single most expensive item on the list of expenses of a farmer because of their financial constraints. The small-scale and semicommercial broiler farmer is faced with the problem of buying small quantities of feed at an inflated price. The problem can be alleviated by groups of small farmers joining co-operative and buying feeds in bulk from suppliers and redistributing to small farmers. The community co-operative can once again play a major role in the distribution of small amounts of feed to its member. The biggest challenge is to provide training with a “hands on” approach. It will be ideal to have a training center where small farmers can be trained in all aspects of poultry production. The training facility will have to operate as a fully operational poultry unit with no risks to bio-security of a commercial enterprise. Training will have to be provided in aspects like hatchery, housing, broilers and record management depending on the farmer’s needs. There is also a need for the research on broiler performance under small-scale and semi commercial farming. Research in this sector of poultry production should look in appropriate feeds, feeding, technology, genotype and performance of genotypes under the system. Emphasis in this study will be on growth patterns and performance of the Ross hybrids as a modern broiler under small-scale and semi commercial management condition. Aspect of the environment such as age, type of feeds and feeding methods, housing, seasons, lighting and genotype will be taken into consideration. The result of the study will provide a realistic database for mathematical modeling of production response and guideline for management planning in small-scale and semi-commercial management condition. Also this result will be important to broiler breeders, to determine the suitability of the modern broiler to small-scale and semi commercial management conditions. The objective of the study was to evaluate the mathematical model that could describe the performance of broilers under the small-scale and semi-commercial management conditions.
- Full Text:
- Date Issued: 2002
- Authors: Prince, Siliga Hulisani
- Date: 2002
- Subjects: Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10963 , http://hdl.handle.net/10948/110 , Broilers (Chickens)
- Description: Introduction : World poultry meat consumption consists of three major segments: broilers, turkeys, and other poultry which includes spent egg layers, spent breeder hens/males, ducks, geese, guinea-fowl, pheasants, quail, ratites, ostriches and emus. Broilers clearly dominate the world poultry consumption contributing about 70 percent to the world poultry market. Turkeys account for about eight percent while other poultry provides the balance of 22 percent (Roenick, 1998). The world broiler industry has been, and continues to be a very dynamic industry. It has been typified with unprecedented growth in terms of production. In 1961, the world produced 6.5 billion broilers. By 1990, the total annual production was a little over 27 billion broilers and by 1996, total world production had grown to 35.3 billion (Van der Sluis., 1999). This represents a fivefold increase in 35 years, and it has not stopped growing since, with world production surpassed 38 billion in 1998 (Van der Sluis., 1999). In Africa the human population was estimated to be 819.9 million, while the total poultry population of the continent was estimated as 1.133 million chickens, 15 million ducks and seven million turkeys in 1998. Production scale being 1.781701 metric tones (MT) of hen eggs, 2.269000 MT of chickens’ meat, 32.824MT of turkey meat and 52.989MT of duck meat (Gueye, 2000). Commercial poultry products are also imported and exported. For example, 12.000 metric tons of poultry meat was estimated to have been exported from South Africa in 1999, whereas 87.000 and 10.000 metric tons of poultry meat was imported into South Africa and Egypt, respectively (Gueye, 2000). In South Africa poultry production is moving toward the 21st century with the potential for increased development being greater than any other sector of the agricultural industry. The small-scale to medium-scale or semi-commercial farmer is becoming a role-player that needs to be serviced by the poultry industry (Fourie, 2000). The industry will have to take up the challenges that are facing an entrepreneur starting with a poultry venture. The poultry industry has developed from a humble beginning at the turn of the century into a multi-million Rand enterprise where protein is provided through the commercial and small marketing sector (Fourie, 2000). Some of the larger commercial companies market three million birds per week. It is estimated that the commercial company sector is responsible for 75 to 85 percent of production and marketing of white meat in this country whereas the small-scale and medium-scale or semi commercial poultry production enterprises provide 20 to 25 percent of the white meat through the sales of slaughtered and live poultry sale to rural, peri-urban and urban settlements (Fourie., 2000). Small-scale and semi-commercial broiler production is developed by entrepreneurs who buy day-old chicks from the big commercial hatcheries, rear them to the age of five to eight weeks and sell them through a marketing network or small outlets. The small-scale consist of farmers who reared 100 t0 1000 chickens per batch and semi-commercial consist of farmers who reared 1000 to 5000 chickens per batch. These types of farmers acquire very little technical backup in terms of a training and services center where assistance is available every day of the week. The broiler industry requires birds that are of commercial crosses selected for their ability to grow very fast and produce a good quality carcass in the shortest time frame. The small farmers purchase broiler stock, as the day-old chicks from commercial hatcheries and reared them up to six to eight weeks before slaughtered or sold as live chickens. The selling of live chickens through a network outlets system is becoming an industry that is growing at a steady pace, as household food security is becoming important to communities (Fourie, 2000). The price of feed is the single most expensive item on the list of expenses of a farmer because of their financial constraints. The small-scale and semicommercial broiler farmer is faced with the problem of buying small quantities of feed at an inflated price. The problem can be alleviated by groups of small farmers joining co-operative and buying feeds in bulk from suppliers and redistributing to small farmers. The community co-operative can once again play a major role in the distribution of small amounts of feed to its member. The biggest challenge is to provide training with a “hands on” approach. It will be ideal to have a training center where small farmers can be trained in all aspects of poultry production. The training facility will have to operate as a fully operational poultry unit with no risks to bio-security of a commercial enterprise. Training will have to be provided in aspects like hatchery, housing, broilers and record management depending on the farmer’s needs. There is also a need for the research on broiler performance under small-scale and semi commercial farming. Research in this sector of poultry production should look in appropriate feeds, feeding, technology, genotype and performance of genotypes under the system. Emphasis in this study will be on growth patterns and performance of the Ross hybrids as a modern broiler under small-scale and semi commercial management condition. Aspect of the environment such as age, type of feeds and feeding methods, housing, seasons, lighting and genotype will be taken into consideration. The result of the study will provide a realistic database for mathematical modeling of production response and guideline for management planning in small-scale and semi-commercial management condition. Also this result will be important to broiler breeders, to determine the suitability of the modern broiler to small-scale and semi commercial management conditions. The objective of the study was to evaluate the mathematical model that could describe the performance of broilers under the small-scale and semi-commercial management conditions.
- Full Text:
- Date Issued: 2002
Process for the preparation of vanillin from a mixed m-cresol/p-cresol stream
- Authors: Buddoo, Subash
- Date: 2002
- Subjects: Vanillin
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10968 , http://hdl.handle.net/10948/138 , Vanillin
- Description: The Vanillin project was undertaken by AECI as it was believed that with the raw materials available from SASOL i.e. cresols, it would be possible to develop a process that would be globally competitive. A process for preparing both vanillin and ethyl vanillin was developed by AECI’s Research and Development Department using a mixed m- and p-cresol stream as feed. The displacement of bromide by methoxide is very successful when using a dimethyl formamide (DMF)/alcohol solvent system and excellent conversions (>95%) and selectivities (>95%) may be obtained. However, the use of DMF, which poses a serious chronic health risk, is unacceptable in this process since the products are intended for use as food and flavouring chemicals. In view of the above the main objectives of this study were: · To find a suitable alternative solvent system, which could produce comparable results while still being economically viable; · To develop an appropriate experimental protocol in the laboratory based on the alternative solvent system; · To determine the important reaction variables by conducting statistically designed experiments; · To optimise the reaction to produce a reproducible and robust experimental protocol; and · To test the reaction thoroughly at bench-scale level and to obtain experimental data for scale-up to pilot plant The most promising alternative solvent system was a methanol/methyl acetate mixture, which produced satisfactory results in the preliminary assessment (conversion of 98.3% and selectivity of 92.0%). DMA and acetonitrile also produced promising results but were not considered for further investigation because of toxicity and cost issues. A set of statistically designed experiments was carried out on the methanol/methyl acetate solvent system where four variables were tested i.e., substrate concentration, temperature, catalyst loading, and methanol to methyl acetate volume ratio. The experimentally determined response surface model showed that the most important variable was catalyst loading (63.2%) for conversion. With respect to selectivity, the most important variables were catalyst loading (31.9%) and methanol to methyl acetate ratio (33.1%). The optimum reaction conditions were as follows: · Temperature: 120°C · Methanol:methyl acetate: 15:1 vol/vol · Catalyst loading: 8 mol % to substrate · Substrate concentration: 22 %m/m on solvent · Catalyst: Copper(I) bromide · Sodium Equivalents: 2.7 wrt substrate · Time: 3 hours The optimum conditions were tested for reproducibility in a 1 Labmax pressure reactor. Replicated reactions, two at a 10% and two at a 20% substrate concentration gave conversions and selectivities all greater than 90%. Although the reaction mixture was a slurry at these concentrations, the reactions were very fast and virtually complete within the first hour (~95% conversion). Initial scale-up studies were conducted in an 8 Parr reactor where five reactions were carried out using the optimum conditions described above. The conversion of substrate and vanillin selectivity was consistently high and compared favourably to the Labmax reactions. The average conversion was 97.3% (96.3 to 98.5%) at an average selectivity of 98.2% (97.4 to 99.1%). A study of the reaction kinetics confirmed that the reaction was first order with respect to the substrate as a plot of substrate concentration versus reaction rate gave a straight line. The rate constant was calculated as 1.1096 k(h-1). The reaction mechanism proposed for the copper assisted nucleophilic aromatic substitution involves the formation of an adduct between sodium methoxide, methyl acetate and copper(I) bromide. The formation of a transient intermediate with the substrate allows intramolecular delivery of the methoxide ion to the aryl moiety through a CuI – CuIII type cycle.
- Full Text:
- Date Issued: 2002
- Authors: Buddoo, Subash
- Date: 2002
- Subjects: Vanillin
- Language: English
- Type: Thesis , Masters , MTech (Chemistry)
- Identifier: vital:10968 , http://hdl.handle.net/10948/138 , Vanillin
- Description: The Vanillin project was undertaken by AECI as it was believed that with the raw materials available from SASOL i.e. cresols, it would be possible to develop a process that would be globally competitive. A process for preparing both vanillin and ethyl vanillin was developed by AECI’s Research and Development Department using a mixed m- and p-cresol stream as feed. The displacement of bromide by methoxide is very successful when using a dimethyl formamide (DMF)/alcohol solvent system and excellent conversions (>95%) and selectivities (>95%) may be obtained. However, the use of DMF, which poses a serious chronic health risk, is unacceptable in this process since the products are intended for use as food and flavouring chemicals. In view of the above the main objectives of this study were: · To find a suitable alternative solvent system, which could produce comparable results while still being economically viable; · To develop an appropriate experimental protocol in the laboratory based on the alternative solvent system; · To determine the important reaction variables by conducting statistically designed experiments; · To optimise the reaction to produce a reproducible and robust experimental protocol; and · To test the reaction thoroughly at bench-scale level and to obtain experimental data for scale-up to pilot plant The most promising alternative solvent system was a methanol/methyl acetate mixture, which produced satisfactory results in the preliminary assessment (conversion of 98.3% and selectivity of 92.0%). DMA and acetonitrile also produced promising results but were not considered for further investigation because of toxicity and cost issues. A set of statistically designed experiments was carried out on the methanol/methyl acetate solvent system where four variables were tested i.e., substrate concentration, temperature, catalyst loading, and methanol to methyl acetate volume ratio. The experimentally determined response surface model showed that the most important variable was catalyst loading (63.2%) for conversion. With respect to selectivity, the most important variables were catalyst loading (31.9%) and methanol to methyl acetate ratio (33.1%). The optimum reaction conditions were as follows: · Temperature: 120°C · Methanol:methyl acetate: 15:1 vol/vol · Catalyst loading: 8 mol % to substrate · Substrate concentration: 22 %m/m on solvent · Catalyst: Copper(I) bromide · Sodium Equivalents: 2.7 wrt substrate · Time: 3 hours The optimum conditions were tested for reproducibility in a 1 Labmax pressure reactor. Replicated reactions, two at a 10% and two at a 20% substrate concentration gave conversions and selectivities all greater than 90%. Although the reaction mixture was a slurry at these concentrations, the reactions were very fast and virtually complete within the first hour (~95% conversion). Initial scale-up studies were conducted in an 8 Parr reactor where five reactions were carried out using the optimum conditions described above. The conversion of substrate and vanillin selectivity was consistently high and compared favourably to the Labmax reactions. The average conversion was 97.3% (96.3 to 98.5%) at an average selectivity of 98.2% (97.4 to 99.1%). A study of the reaction kinetics confirmed that the reaction was first order with respect to the substrate as a plot of substrate concentration versus reaction rate gave a straight line. The rate constant was calculated as 1.1096 k(h-1). The reaction mechanism proposed for the copper assisted nucleophilic aromatic substitution involves the formation of an adduct between sodium methoxide, methyl acetate and copper(I) bromide. The formation of a transient intermediate with the substrate allows intramolecular delivery of the methoxide ion to the aryl moiety through a CuI – CuIII type cycle.
- Full Text:
- Date Issued: 2002
Relationship between temparament and linear body parameters of beef cattle under communal grazing system
- Authors: Nekhofhe, Avhasei Justice
- Date: 2002
- Subjects: Cattle , Grazing
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10965 , http://hdl.handle.net/10948/113 , Cattle , Grazing
- Description: Introduction : Indigenous cattle are mostly reared where the traditional farming is practiced including Northern Region of South Africa. The majority of farmers own Nguni type of cattle although other breeds are found in the region. They perform well under harsh conditions and they seem to produce better under intensive management. However, communal farmers are becoming aware and interested in indigenous breeds especially Nguni cattle type because of their adaptability. Beef cattle production and research require constant movement and handling of livestock (Erf et al., 1992 & Grandin, 1993). Dipping, castration, branding, ear-tagging and counting is commonly practiced as beef cattle management norms hence dehorning are partly practiced as some of the animals had horns which were disturbing them to pass through crushpens and as a result they increased bruising to other animals. At Matatani and Muledzhi areas animals including calves are packed in the crushpens, counted, weighed and temperament traits were also measured on the scale and this is done in order to document the information for this research and future use. Temperament is a behavioural response of animals as handled by man. It is important to be measured in beef cattle farmers under communal grazing conditions as it reduces farmer’s profit and it makes the stock man’s work unpleasant. Therefore, it is also needs to be improved for the improvement of beef cattle schemes. Management system of communal farmers was not conducive to better quality meat due to horns in some animals which ultimately cause bruising in other animals. However, exotic bulls were bought at random for the improvement of other breed types found in these two areas. The focus of this study is almost in Nguni cattle type managed under communal grazing system. Lastly, the purpose of this study is to determine behavioral response of beef cattle under communal management system at their dipping tanks.
- Full Text:
- Date Issued: 2002
- Authors: Nekhofhe, Avhasei Justice
- Date: 2002
- Subjects: Cattle , Grazing
- Language: English
- Type: Thesis , Masters , MTech (Agricultural Management)
- Identifier: vital:10965 , http://hdl.handle.net/10948/113 , Cattle , Grazing
- Description: Introduction : Indigenous cattle are mostly reared where the traditional farming is practiced including Northern Region of South Africa. The majority of farmers own Nguni type of cattle although other breeds are found in the region. They perform well under harsh conditions and they seem to produce better under intensive management. However, communal farmers are becoming aware and interested in indigenous breeds especially Nguni cattle type because of their adaptability. Beef cattle production and research require constant movement and handling of livestock (Erf et al., 1992 & Grandin, 1993). Dipping, castration, branding, ear-tagging and counting is commonly practiced as beef cattle management norms hence dehorning are partly practiced as some of the animals had horns which were disturbing them to pass through crushpens and as a result they increased bruising to other animals. At Matatani and Muledzhi areas animals including calves are packed in the crushpens, counted, weighed and temperament traits were also measured on the scale and this is done in order to document the information for this research and future use. Temperament is a behavioural response of animals as handled by man. It is important to be measured in beef cattle farmers under communal grazing conditions as it reduces farmer’s profit and it makes the stock man’s work unpleasant. Therefore, it is also needs to be improved for the improvement of beef cattle schemes. Management system of communal farmers was not conducive to better quality meat due to horns in some animals which ultimately cause bruising in other animals. However, exotic bulls were bought at random for the improvement of other breed types found in these two areas. The focus of this study is almost in Nguni cattle type managed under communal grazing system. Lastly, the purpose of this study is to determine behavioral response of beef cattle under communal management system at their dipping tanks.
- Full Text:
- Date Issued: 2002
The effect of a soil-amending hydrogel on Eucalyptus grandis establishment practices in the Zululand forestry region
- Authors: Viero, Paul Walter Mario
- Date: 2002
- Subjects: Eucalyptus grandis
- Language: English
- Type: Thesis , Masters , MTech (Forestry)
- Identifier: vital:10973 , http://hdl.handle.net/10948/90 , Eucalyptus grandis
- Description: To ensure acceptable survival and initial growth of Eucalyptus grandis clonal and clonal hybrid material planted in the cool temperate and sub-tropical climates of Zululand (KwaZulu-Natal, South Africa) the planting seasons are currently restricted to the winter or summer months respectively. The Zululand coast (sub-tropical climate) experiences traditionally hot and humid summers and as a result Eucalyptus planting is largely restricted to the cool and dry winter months when survival is acceptable (90- 95%). In comparison, the Zululand interior (cool temperate climate) experiences moderately cooler summers followed by drier winters. As a result, the Eucalyptus planting season is reversed to that of the Zululand coast, with most of the planting taking place during the summer months with little or no planting occurring during winter. To ensure adequate transplant survival during and beyond these periods, transplants are planted with large volumes of water at a high cost. To determine whether it was possible to significantly reduce current water volumes at planting and therefore reduce costs, and to potentially extend the current planting seasons, two field trials were initiated during traditionally unsuitable planting periods (winter months for the Zululand interior and summer months for the Zululand coast). These trials were established near Kwambonambi on the Zululand coast and at Ntonjaneni in the Zululand interior. Five levels of water were combined with five levels of hydrogel and applied to the pit at planting in a 5 x 5 factorial treatment design for both trials. The tree variates of mortality, height, groundline diameter, crown diameter, corrected leaf surface index (LSIC) and corrected biomass index (BIC) were assessed at regular intervals until the final measurement dates (118 and 128 days after planting for the Ntonjaneni and Kwambonambi trials respectively). For the Kwambonambi trial, the response of transplant survival to the application water was highly significant (p<0,01) 128 days after planting, but not to the application of the hydrogel. Transplant survival nevertheless conformed to the silviculturally accepted norms of 90-95% using the hydrogel, thus water volumes could be significantly reduced without negatively affecting current survival standards. The lack of the expected response of significantly reduced transplant survival to increasing levels of hydrogel could possibly be attributed to the significant rainfall event (146 mm) that fell two days after trial initiation. Increasing levels of both water and hydrogel resulted in significantly enhanced growth (LSIC and BIC: p<0.01) for the final measurement date. For the Ntonjaneni trial, there was a significant (p<0,01) interaction between hydrogel and water, 118 days after planting. There were significant (p<0,01) differences between water only and all hydrogel treatments, with the hydrogel treatments performing significantly better. Optimum transplant survival for water only treatments was 50% using 4000 ml water while that for hydrogel treatments was 100% using 6 g hydrogel with 1000 ml of water and 12 g hydrogel with 2000 ml of water. The variates, corrected leaf surface index and corrected biomass index indicated that tree growth was significantly enhanced by the addition of a hydrogel over all levels of water. A pot trial was subsequently implemented to ascertain whether the significant increases obtained for initial transplant growth for the sandy clay loam soils of Ntonjaneni were due to an initial but unsustainable positive response of the roots to the presence of the hydrogel, or whether root growth was sustainably advantaged by the presence of the hydrogel. There was a highly significant (p<0,01) response of root biomass and above ground biomass over all levels of hydrogel, including a significant positive linear (p<0,01) relationship between increased root biomass and above ground biomass. This clearly indicated that initial root growth was not negatively affected by the addition of the soil-amending hydrogel Stockosorb 400K.
- Full Text:
- Date Issued: 2002
- Authors: Viero, Paul Walter Mario
- Date: 2002
- Subjects: Eucalyptus grandis
- Language: English
- Type: Thesis , Masters , MTech (Forestry)
- Identifier: vital:10973 , http://hdl.handle.net/10948/90 , Eucalyptus grandis
- Description: To ensure acceptable survival and initial growth of Eucalyptus grandis clonal and clonal hybrid material planted in the cool temperate and sub-tropical climates of Zululand (KwaZulu-Natal, South Africa) the planting seasons are currently restricted to the winter or summer months respectively. The Zululand coast (sub-tropical climate) experiences traditionally hot and humid summers and as a result Eucalyptus planting is largely restricted to the cool and dry winter months when survival is acceptable (90- 95%). In comparison, the Zululand interior (cool temperate climate) experiences moderately cooler summers followed by drier winters. As a result, the Eucalyptus planting season is reversed to that of the Zululand coast, with most of the planting taking place during the summer months with little or no planting occurring during winter. To ensure adequate transplant survival during and beyond these periods, transplants are planted with large volumes of water at a high cost. To determine whether it was possible to significantly reduce current water volumes at planting and therefore reduce costs, and to potentially extend the current planting seasons, two field trials were initiated during traditionally unsuitable planting periods (winter months for the Zululand interior and summer months for the Zululand coast). These trials were established near Kwambonambi on the Zululand coast and at Ntonjaneni in the Zululand interior. Five levels of water were combined with five levels of hydrogel and applied to the pit at planting in a 5 x 5 factorial treatment design for both trials. The tree variates of mortality, height, groundline diameter, crown diameter, corrected leaf surface index (LSIC) and corrected biomass index (BIC) were assessed at regular intervals until the final measurement dates (118 and 128 days after planting for the Ntonjaneni and Kwambonambi trials respectively). For the Kwambonambi trial, the response of transplant survival to the application water was highly significant (p<0,01) 128 days after planting, but not to the application of the hydrogel. Transplant survival nevertheless conformed to the silviculturally accepted norms of 90-95% using the hydrogel, thus water volumes could be significantly reduced without negatively affecting current survival standards. The lack of the expected response of significantly reduced transplant survival to increasing levels of hydrogel could possibly be attributed to the significant rainfall event (146 mm) that fell two days after trial initiation. Increasing levels of both water and hydrogel resulted in significantly enhanced growth (LSIC and BIC: p<0.01) for the final measurement date. For the Ntonjaneni trial, there was a significant (p<0,01) interaction between hydrogel and water, 118 days after planting. There were significant (p<0,01) differences between water only and all hydrogel treatments, with the hydrogel treatments performing significantly better. Optimum transplant survival for water only treatments was 50% using 4000 ml water while that for hydrogel treatments was 100% using 6 g hydrogel with 1000 ml of water and 12 g hydrogel with 2000 ml of water. The variates, corrected leaf surface index and corrected biomass index indicated that tree growth was significantly enhanced by the addition of a hydrogel over all levels of water. A pot trial was subsequently implemented to ascertain whether the significant increases obtained for initial transplant growth for the sandy clay loam soils of Ntonjaneni were due to an initial but unsustainable positive response of the roots to the presence of the hydrogel, or whether root growth was sustainably advantaged by the presence of the hydrogel. There was a highly significant (p<0,01) response of root biomass and above ground biomass over all levels of hydrogel, including a significant positive linear (p<0,01) relationship between increased root biomass and above ground biomass. This clearly indicated that initial root growth was not negatively affected by the addition of the soil-amending hydrogel Stockosorb 400K.
- Full Text:
- Date Issued: 2002
The effect of nutrient levels and ratios on the growth of Microcystis aeruginosa and microcystin production
- Authors: Sember, Craig Stewart
- Date: 2002
- Subjects: Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11076 , http://hdl.handle.net/10948/287 , Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Description: This study reports the findings on the effect of nitrates and phosphates on the biomass and toxin production of various strains of the unicellular non-nitrogen fixing cyanobacterium, Microcystis aeruginosa. The occurrence of blooms of Microcystis aeruginosa and microcystin in freshwater impoundments across the globe has been on the increase lately due to increased levels of eutrophication, resulting in human and animal deaths and illness, as well as drinking and recreational water foulment. A range of environmental factors have been shown to effect growth and microcystin production. Existing literature however is somewhat contradictory as to the effects of these physical and chemical factors on toxin production. Therefore Microcystis aeruginosa strains were cultured under batch and continuous conditions to determine the effect of nitrate and phosphate concentrations and ratios on biomass and toxin production. Cultures were analysed with regards to internal nutrient stores, biomass production, nutrient depletion, photosynthetic efficiency and microcystin production. Results showed that microcystin production correlated to growth rate, photosynthetic efficiency and internal nitrogen stores and that an optimal N:P ratio was associated with microcystin levels, growth rate and photosynthetic efficiency. Results therefore led to the conclusion that the nitrogen, carbon, and phosphate balance within the cell is closely associated with microcystin production. Whether or not microcystin is produced to maintain this balance or produced as a function of this balance remains to be determined.
- Full Text:
- Date Issued: 2002
- Authors: Sember, Craig Stewart
- Date: 2002
- Subjects: Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11076 , http://hdl.handle.net/10948/287 , Microcystis aeruginosa -- Toxicology , Nitrates , Microcystins
- Description: This study reports the findings on the effect of nitrates and phosphates on the biomass and toxin production of various strains of the unicellular non-nitrogen fixing cyanobacterium, Microcystis aeruginosa. The occurrence of blooms of Microcystis aeruginosa and microcystin in freshwater impoundments across the globe has been on the increase lately due to increased levels of eutrophication, resulting in human and animal deaths and illness, as well as drinking and recreational water foulment. A range of environmental factors have been shown to effect growth and microcystin production. Existing literature however is somewhat contradictory as to the effects of these physical and chemical factors on toxin production. Therefore Microcystis aeruginosa strains were cultured under batch and continuous conditions to determine the effect of nitrate and phosphate concentrations and ratios on biomass and toxin production. Cultures were analysed with regards to internal nutrient stores, biomass production, nutrient depletion, photosynthetic efficiency and microcystin production. Results showed that microcystin production correlated to growth rate, photosynthetic efficiency and internal nitrogen stores and that an optimal N:P ratio was associated with microcystin levels, growth rate and photosynthetic efficiency. Results therefore led to the conclusion that the nitrogen, carbon, and phosphate balance within the cell is closely associated with microcystin production. Whether or not microcystin is produced to maintain this balance or produced as a function of this balance remains to be determined.
- Full Text:
- Date Issued: 2002
The effect of selenium in the detoxification of the microcystin hepatotoxins
- Authors: Downs, Kerry
- Date: 2002
- Subjects: Cynaobacterial toxins , Microcystins , Selenium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11070 , http://hdl.handle.net/10948/284 , Cynaobacterial toxins , Microcystins , Selenium
- Description: Blooms of cyanobacteria have been known to cause illness in humans and death in wild and domestic animals. One of the toxins produced by cyanobacteria is microcystin, which is a potent hepatotoxin. Microcystin is taken up by bile acid transporters in the intestine and transported into the liver. After exposure to acute doses of microcystin, severe haemorrhage has been observed along with apoptotic and necrotic hepatocytes. The cytoskeletal structure of the hepatocytes is disrupted and oxidative stress is induced. Selenium, a known anti-oxidant, has been shown to induce increased activity of glutathione peroxidase. Glutathione peroxidase removes peroxides from cells protecting them from oxidative stress. This study set out to determine if selenium could play a role in preventing the damage to mice livers due to microcystin toxin. The protective role of selenium was explored in three main studies: in the first study, the ability of selenium to increase the survival time of mice exposed to a lethal dose of toxin was determined. In the second study the mice were exposed to sublethal chronic doses of toxin over 30 days. The ability of selenium to minimise liver damage under these conditions was determined. The final study investigated the mechanism of the protective effect of selenium. The results of the first study suggested that selenium could extend survival time. In the second study the selenium supplemented mice showed a reduction in the extent of the increase in liver weight and a decrease in the amount of lipid peroxidation induced compared to the mice that received only toxin. The histology of the selenium supplemented mice also showed a decrease in the severity and amount of morphological changes in the liver. The third study indicated that the protection shown by selenium might be mediated by an increase in the glutathione peroxidase (GPX) activity in selenium supplemented mice. This increase in GPX activity would increase the removal of the lipid hydroperoxides and prevent the damage they would cause in the cell. A further result indicated an increase in glutathione S-transferase in only the toxin control mice when compared to the selenium supplemented and control mice. ii In conclusion selenium offers protection against microcystin but further studies need to be done to provide statistically valid results to clarify the level of protection.
- Full Text:
- Date Issued: 2002
- Authors: Downs, Kerry
- Date: 2002
- Subjects: Cynaobacterial toxins , Microcystins , Selenium
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11070 , http://hdl.handle.net/10948/284 , Cynaobacterial toxins , Microcystins , Selenium
- Description: Blooms of cyanobacteria have been known to cause illness in humans and death in wild and domestic animals. One of the toxins produced by cyanobacteria is microcystin, which is a potent hepatotoxin. Microcystin is taken up by bile acid transporters in the intestine and transported into the liver. After exposure to acute doses of microcystin, severe haemorrhage has been observed along with apoptotic and necrotic hepatocytes. The cytoskeletal structure of the hepatocytes is disrupted and oxidative stress is induced. Selenium, a known anti-oxidant, has been shown to induce increased activity of glutathione peroxidase. Glutathione peroxidase removes peroxides from cells protecting them from oxidative stress. This study set out to determine if selenium could play a role in preventing the damage to mice livers due to microcystin toxin. The protective role of selenium was explored in three main studies: in the first study, the ability of selenium to increase the survival time of mice exposed to a lethal dose of toxin was determined. In the second study the mice were exposed to sublethal chronic doses of toxin over 30 days. The ability of selenium to minimise liver damage under these conditions was determined. The final study investigated the mechanism of the protective effect of selenium. The results of the first study suggested that selenium could extend survival time. In the second study the selenium supplemented mice showed a reduction in the extent of the increase in liver weight and a decrease in the amount of lipid peroxidation induced compared to the mice that received only toxin. The histology of the selenium supplemented mice also showed a decrease in the severity and amount of morphological changes in the liver. The third study indicated that the protection shown by selenium might be mediated by an increase in the glutathione peroxidase (GPX) activity in selenium supplemented mice. This increase in GPX activity would increase the removal of the lipid hydroperoxides and prevent the damage they would cause in the cell. A further result indicated an increase in glutathione S-transferase in only the toxin control mice when compared to the selenium supplemented and control mice. ii In conclusion selenium offers protection against microcystin but further studies need to be done to provide statistically valid results to clarify the level of protection.
- Full Text:
- Date Issued: 2002