Development of a Mobile-Based Livestock Breeding Management System for Communal Farmers in Eastern Cape, South Africa
- Authors: Aduradola, Oluwatobi John
- Date: 2021-05
- Subjects: Mobile communication systems , Mobile computing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20600 , vital:46364
- Description: More than a decade ago, South Africa realized the significance of Information and Communication Technology (ICT) and its use in delivering several services; there are still several challenges that block implementing an ICT-based system to deliver services and information better. Since communal development is still one of the country's biggest concerns, ICT is among several advances and views recognized for speeding up communal development and providing for the country's economic growth. This research aimed to investigate and implement ICT tools for communal farmers in the context of information needs and information dissemination. The research concentrated on the technical tools and skills needed to implement an ICT tool for communal farmers and other stakeholders. A prototyping methodology was used in this research in the development of the required prototype. A study was then conducted to understand the present state and the information needs of communal farmers where the questionnaire was administered. The research results demonstrated the critical need for communal farmers to get information timely at their convenience. A mobile database system was then designed and developed that suits communal farmers' nature. This system was an offline android application that assists with their information need and its dissemination, and it allows the sharing of the communal farmer's data to other stakeholders. , Thesis (MSc) (Computer Science)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-05
- Authors: Aduradola, Oluwatobi John
- Date: 2021-05
- Subjects: Mobile communication systems , Mobile computing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20600 , vital:46364
- Description: More than a decade ago, South Africa realized the significance of Information and Communication Technology (ICT) and its use in delivering several services; there are still several challenges that block implementing an ICT-based system to deliver services and information better. Since communal development is still one of the country's biggest concerns, ICT is among several advances and views recognized for speeding up communal development and providing for the country's economic growth. This research aimed to investigate and implement ICT tools for communal farmers in the context of information needs and information dissemination. The research concentrated on the technical tools and skills needed to implement an ICT tool for communal farmers and other stakeholders. A prototyping methodology was used in this research in the development of the required prototype. A study was then conducted to understand the present state and the information needs of communal farmers where the questionnaire was administered. The research results demonstrated the critical need for communal farmers to get information timely at their convenience. A mobile database system was then designed and developed that suits communal farmers' nature. This system was an offline android application that assists with their information need and its dissemination, and it allows the sharing of the communal farmer's data to other stakeholders. , Thesis (MSc) (Computer Science)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-05
The optimisation of transportation methods for abalone (Haliotis midae Linnaeus, 1758 (Mollusca: Gastropoda)) larvae
- Authors: Bajaba, Sharone
- Date: 2021-02
- Subjects: Abalone fisheries , Haliotis midae , Abalone culture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20561 , vital:46124
- Description: Sea ranching has been identified as a viable method for enhancing the natural stock of overexploited abalone (Haliotis midae). Currently, this process involves transporting live juvenile H. midae to the seeding site where they are released onto the reef however this is both costly and logistically problematic. Transportation of abalone larvae is another cost-effective option, as they are cheaper to produce and can be transported at high densities. A suitable larval transport method is required to minimise larval mortalities and stresses that might compromise settlement. A series of simulated experiments were conducted to optimise transportation systems of abalone (Haliotis midae) larvae. First, two potential transportation modes (Wet (W) and Dry (D) transportation) conducted at a cooler (14˚C) and average ambient (18˚C) temperatures with six replicates of each were compared with control treatments (six replicates) that were not subjected to transport, kept in water at 18˚C. Eighty hours post-initial settlement, the 14W treatment had significantly lower settlement (p=0.03) than the other three treatments (14D, 18W, 18D) and the Control. The Dry method was the prefered method to transport larvae as it is logistically simpler to employ. The second experiment investigated the effect of different stocking densities (200, 400 and 800 larvae cm-2) for the Dry method 18˚C over two transit periods (six and twelve hours) on post-transport settlement and post-settlement survival of H. midae larvae. Compared to the control, there was no difference in the number of settled larvae (p=0.368) and larvae still swimming (p=0.835) across all treatments. This suggested that H. midae larvae can be transported for twelve hours at 800 larvae cm-2 without compromising post-transport settlement or survival. This study’s results and recommendations can be used by abalone farms when there is a need to move abalone H. midae larvae between farms or to seeding sites for sea ranching. Furthermore, other researchers can use these results as a benchmark for larval transportation studies of H. midae and other abalone species. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Bajaba, Sharone
- Date: 2021-02
- Subjects: Abalone fisheries , Haliotis midae , Abalone culture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20561 , vital:46124
- Description: Sea ranching has been identified as a viable method for enhancing the natural stock of overexploited abalone (Haliotis midae). Currently, this process involves transporting live juvenile H. midae to the seeding site where they are released onto the reef however this is both costly and logistically problematic. Transportation of abalone larvae is another cost-effective option, as they are cheaper to produce and can be transported at high densities. A suitable larval transport method is required to minimise larval mortalities and stresses that might compromise settlement. A series of simulated experiments were conducted to optimise transportation systems of abalone (Haliotis midae) larvae. First, two potential transportation modes (Wet (W) and Dry (D) transportation) conducted at a cooler (14˚C) and average ambient (18˚C) temperatures with six replicates of each were compared with control treatments (six replicates) that were not subjected to transport, kept in water at 18˚C. Eighty hours post-initial settlement, the 14W treatment had significantly lower settlement (p=0.03) than the other three treatments (14D, 18W, 18D) and the Control. The Dry method was the prefered method to transport larvae as it is logistically simpler to employ. The second experiment investigated the effect of different stocking densities (200, 400 and 800 larvae cm-2) for the Dry method 18˚C over two transit periods (six and twelve hours) on post-transport settlement and post-settlement survival of H. midae larvae. Compared to the control, there was no difference in the number of settled larvae (p=0.368) and larvae still swimming (p=0.835) across all treatments. This suggested that H. midae larvae can be transported for twelve hours at 800 larvae cm-2 without compromising post-transport settlement or survival. This study’s results and recommendations can be used by abalone farms when there is a need to move abalone H. midae larvae between farms or to seeding sites for sea ranching. Furthermore, other researchers can use these results as a benchmark for larval transportation studies of H. midae and other abalone species. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
Caregivers’ Knowledge, Attitude and Practices (KAP) of breastfeeding and prevention of mother-to-child transmission (pmtct) in Nelson Mandela Bay Health District: impact of a community dialogue intervention
- Authors: Barnard, Marné
- Date: 2021-04
- Subjects: Breastfeeding -- Transmission -- South Africa -- Nelson Mandela Bay Municipality -- Prevention , Communicable diseases -- South Africa -- Nelson Mandela Bay Municipality -- Prevention
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/50988 , vital:43196
- Description: Background: Globally, childhood mortality remains high with 5.3 million deaths occurring in children under the age of five years in 2018. Of these deaths, 43 000 were children from South Africa, with malnutrition being a significant risk factor contributing to disease and death. Breastfeeding has many benefits and reduces childhood mortality. Current primary health care strategies in South Africa are not having an adequate impact on breastfeeding rates, therefore appropriate messages need to be targeted at the community in order to improve breastfeeding rates. Participatory approaches have been identified as an effective tool to promote child health. Community dialogues led by skilled Early Childhood Development (ECD) mentors from the community might be key in order to improve caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and Prevention of Mother to Child Transmission of HIV (PMTCT). Aim : The aim of the study was to explore the potential effects of community dialogues, led by ECD mentors, on caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and prevention of mother-to-child transmission of HIV (PMTCT). Methods: Quasi-experimental design was used to compare the KAP of caregivers in relation to breastfeeding and PMTCT. Convenience sampling was used to include 63 caregivers who participated in the umbrella project (mixed-methods) and 61 caregivers who did not participate in the project. Ethics approval was obtained from the Nelson Mandela University Faculty Postgraduate Studies Committee. Participants had to provide written informed consent. A skilled interviewer used a structured questionnaire to assess the KAP of all participants. Data were captured on an Excel spreadsheet and analysed in collaboration with a statistician, who analysed the data using Microsoft Excel. Descriptive statistics were applied to numerical data, while frequency distributions were applied to categorical data. Correlations were calculated to describe the degree of relationship between two variables. Inferential statistic tests, conducted at a significance level of 0,05, were used for hypothesis testing. The t-test and analysis of variance (ANOVA) were used in tests involving mean values. Scheffé’s test was used in ANOVA to determine between which of the groups a statistically significant difference was found. Results: In the intervention group more than double the number participants (35%; n=22) had a good knowledge of breastfeeding when compared to participants (15%; n=9) in the control group. Additionally, the intervention group participants displayed better attitudes towards the RtHB (p=0.02). However, control group participants showed better knowledge towards the HIV (p=0.01). Finally, results suggest that in the intervention group, participants (33%; n=21) were more likely to have a good total KAP score in relation to breastfeeding and PMTCT when compared to participants (11%; n=7) in the control group. In the intervention group, better knowledge correlated with good practices relating to the RtHB (r=0.27). However, in the control group, positive attitudes correlated with better practices relating to HIV (r=0.29). Younger intervention group participants displayed better breastfeeding related practices (p=0.011). Additionally, younger control group participants displayed better knowledge of breastfeeding (p=0.001). Intervention group participants whose choices were influenced by their husband/ partner (p =0.016) and/ or radio/ magazines/ newspapers (p=0.004) had positive attitudes towards HIV. Control group participants whose choices were influenced by their other family members (p=0.043) and/ or clinic staff (p=0.016) had good knowledge of sharing their knowledge. Conclusions and recommendations: The intervention group displayed a better knowledge of breastfeeding as well as more desirable attitudes towards the RtHB, when compared to the control group. However, the control group displayed better knowledge of HIV, which could be due to the control group having more younger participants when compared to the intervention group. The community dialogue intervention had a positive impact on caregivers KAP relating to breastfeeding and PMTCT, which included all the different categories combined. In order to improve the Sakha Esethu intervention programme, managers need to provide guidance to the mentors on how to initiate conversations regarding HIV and discuss the stigma associated with HIV and breastfeeding. Results showed that including grandmothers and husbands or partners in the target population will benefit the intervention. Additionally, the use of radio needs to be considered especially for discussions regarding HIV. Lastly, sustainability needs to be improved due to many participants not remembering certain detail regarding topics that was discussed. , Thesis (MSc) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Barnard, Marné
- Date: 2021-04
- Subjects: Breastfeeding -- Transmission -- South Africa -- Nelson Mandela Bay Municipality -- Prevention , Communicable diseases -- South Africa -- Nelson Mandela Bay Municipality -- Prevention
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/50988 , vital:43196
- Description: Background: Globally, childhood mortality remains high with 5.3 million deaths occurring in children under the age of five years in 2018. Of these deaths, 43 000 were children from South Africa, with malnutrition being a significant risk factor contributing to disease and death. Breastfeeding has many benefits and reduces childhood mortality. Current primary health care strategies in South Africa are not having an adequate impact on breastfeeding rates, therefore appropriate messages need to be targeted at the community in order to improve breastfeeding rates. Participatory approaches have been identified as an effective tool to promote child health. Community dialogues led by skilled Early Childhood Development (ECD) mentors from the community might be key in order to improve caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and Prevention of Mother to Child Transmission of HIV (PMTCT). Aim : The aim of the study was to explore the potential effects of community dialogues, led by ECD mentors, on caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and prevention of mother-to-child transmission of HIV (PMTCT). Methods: Quasi-experimental design was used to compare the KAP of caregivers in relation to breastfeeding and PMTCT. Convenience sampling was used to include 63 caregivers who participated in the umbrella project (mixed-methods) and 61 caregivers who did not participate in the project. Ethics approval was obtained from the Nelson Mandela University Faculty Postgraduate Studies Committee. Participants had to provide written informed consent. A skilled interviewer used a structured questionnaire to assess the KAP of all participants. Data were captured on an Excel spreadsheet and analysed in collaboration with a statistician, who analysed the data using Microsoft Excel. Descriptive statistics were applied to numerical data, while frequency distributions were applied to categorical data. Correlations were calculated to describe the degree of relationship between two variables. Inferential statistic tests, conducted at a significance level of 0,05, were used for hypothesis testing. The t-test and analysis of variance (ANOVA) were used in tests involving mean values. Scheffé’s test was used in ANOVA to determine between which of the groups a statistically significant difference was found. Results: In the intervention group more than double the number participants (35%; n=22) had a good knowledge of breastfeeding when compared to participants (15%; n=9) in the control group. Additionally, the intervention group participants displayed better attitudes towards the RtHB (p=0.02). However, control group participants showed better knowledge towards the HIV (p=0.01). Finally, results suggest that in the intervention group, participants (33%; n=21) were more likely to have a good total KAP score in relation to breastfeeding and PMTCT when compared to participants (11%; n=7) in the control group. In the intervention group, better knowledge correlated with good practices relating to the RtHB (r=0.27). However, in the control group, positive attitudes correlated with better practices relating to HIV (r=0.29). Younger intervention group participants displayed better breastfeeding related practices (p=0.011). Additionally, younger control group participants displayed better knowledge of breastfeeding (p=0.001). Intervention group participants whose choices were influenced by their husband/ partner (p =0.016) and/ or radio/ magazines/ newspapers (p=0.004) had positive attitudes towards HIV. Control group participants whose choices were influenced by their other family members (p=0.043) and/ or clinic staff (p=0.016) had good knowledge of sharing their knowledge. Conclusions and recommendations: The intervention group displayed a better knowledge of breastfeeding as well as more desirable attitudes towards the RtHB, when compared to the control group. However, the control group displayed better knowledge of HIV, which could be due to the control group having more younger participants when compared to the intervention group. The community dialogue intervention had a positive impact on caregivers KAP relating to breastfeeding and PMTCT, which included all the different categories combined. In order to improve the Sakha Esethu intervention programme, managers need to provide guidance to the mentors on how to initiate conversations regarding HIV and discuss the stigma associated with HIV and breastfeeding. Results showed that including grandmothers and husbands or partners in the target population will benefit the intervention. Additionally, the use of radio needs to be considered especially for discussions regarding HIV. Lastly, sustainability needs to be improved due to many participants not remembering certain detail regarding topics that was discussed. , Thesis (MSc) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
Perceptions of blended learning by academic staff in the Health Sciences Faculty at Nelson Mandela University
- Authors: Barry, Janet
- Date: 2021-04
- Subjects: Blended learning -- South Africa -- Nelson Mandela Bay Municipality -- Faculty , Medical sciences -- South Africa -- Nelson Mandela Bay Municipality -- Faculty
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/50999 , vital:43199
- Description: Numerous studies have investigated the use of blended learning by academic staff at tertiary institutions. The purpose of this study was to examine the perceptions of blended learning by academic staff at the Faculty of Health Sciences at Nelson Mandela University. A related objective was to identify barriers and facilitators to the adoption of blended learning by academic staff. A sequential, exploratory mixed-methods design was adopted for this study whereby Phase 1 (focus groups) was used to inform Phase 2 (questionnaire) of the study. Results were analysed from both phases and organised according to strategies, support, and structure of a blended learning adoption framework. Existing support structures to assist with blended learning adoption, understanding of what blended learning is, confidence in using blended learning tools, and time to attend training were some of the findings of the study. The researcher concluded that academic staff in the Health Sciences Faculty at Nelson Mandela University are positioned in the second stage of the blended learning adoption framework, namely the early adoption stage of blended learning. These findings imply that existing strategies and support within the Faculty and the University need to be further developed, and structures put into place to move to an advanced stage of adoption of blended learning by academic staff in Health Sciences Faculty of Nelson Mandela University. , Thesis (MPharm) -- Faculty of Health Sciences, Pharmacy, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Barry, Janet
- Date: 2021-04
- Subjects: Blended learning -- South Africa -- Nelson Mandela Bay Municipality -- Faculty , Medical sciences -- South Africa -- Nelson Mandela Bay Municipality -- Faculty
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/50999 , vital:43199
- Description: Numerous studies have investigated the use of blended learning by academic staff at tertiary institutions. The purpose of this study was to examine the perceptions of blended learning by academic staff at the Faculty of Health Sciences at Nelson Mandela University. A related objective was to identify barriers and facilitators to the adoption of blended learning by academic staff. A sequential, exploratory mixed-methods design was adopted for this study whereby Phase 1 (focus groups) was used to inform Phase 2 (questionnaire) of the study. Results were analysed from both phases and organised according to strategies, support, and structure of a blended learning adoption framework. Existing support structures to assist with blended learning adoption, understanding of what blended learning is, confidence in using blended learning tools, and time to attend training were some of the findings of the study. The researcher concluded that academic staff in the Health Sciences Faculty at Nelson Mandela University are positioned in the second stage of the blended learning adoption framework, namely the early adoption stage of blended learning. These findings imply that existing strategies and support within the Faculty and the University need to be further developed, and structures put into place to move to an advanced stage of adoption of blended learning by academic staff in Health Sciences Faculty of Nelson Mandela University. , Thesis (MPharm) -- Faculty of Health Sciences, Pharmacy, 2021
- Full Text:
- Date Issued: 2021-04
Agro-morphological characterization and investigations into the response of yellow Quality Protein Maize (QPM) genotypes to low soil pH
- Authors: Bobotyana, Simthandile
- Date: 2021-04
- Subjects: Corn as food , Plants--Drought tolerance
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20528 , vital:46087
- Description: Maize (Zea mays L) is an essential food grain crop in South Africa and is mainly grown under rainfed conditions by small-scale farmers. Quality Protein Maize (QPM) contains a higher level of two essential amino acids, tryptophan, and lysine when equated to normal maize. Therefore, the QPM in communities is a vital cereal where maize is a staple crop. Among the significant abiotic stresses that adversely affect maize productivity, such as soil fertility. Low soil pH or soil acidity results in nutrient deficiencies, as well as the toxicity of minerals such as manganese (Mn), aluminium (Al), and iron (Fe). The objectives of the study were to: (1) Characterize yellow QPM inbred lines and testcross hybrids for agro-morphological variability under optimum conditions, (2) Determine the response of QPM inbred lines and testcross hybrids to Al toxicity, and (3) Estimate the combining ability of QPM genotypes for the tolerance to Al toxicity. The laboratory assessment for tolerance to Al toxicity revealed a negative relationship between relative root growth RRG and hematoxylin staining; among the inbred lines, the genotype 8Y, 17Y, and 7Y had the highest RRG and net root growth (NRG) values and exhibited the highest tolerance to Al tolerance with the least hematoxylin staining. Inbred lines 12Y, 16Y, 19Y, 2Y, 20Y, 24Y, 30Y, 27Y, 34Y, 4Y, and 11Y were moderately tolerant. On the other hand, inbred lines 13Y, 18Y, and QSY5 had the least RRG were susceptible. Forty-four testcross hybrids were generated by crossing 22 QPM inbred lines with two open-pollinated variety (OPV) testers. These hybrids were evaluated together with five checks for different agronomic traits. The hybrids were planted in the summer season of 2017/18 and 2018/19 under rain-fed conditions 7 x 7 alpha lattice design was used. The significant variations among the genotypes for grain yield and most other agronomic traits under optimum, acidic conditions and across environments on combined analysis of variance. The hybrid was 14Y x NC (3.22 t/ha), followed by QSY5 x NC (3.02 t/ha) showed the highest yielding. The mean plant height was 226.23 cm, with ranges 197.60 to 254.85cm. The number of kernels per ear was 35, with a range from 29.45 to 40.25. Conversely, the mean ear length was 19.30 cm with a range of 17.65 to 20.95 cm, and the mean ear diameter ranged from 13,50 to 16,05 cm. The highest yielding hybrid, according to ranking, was 14Y x NC (2,93t/ha), which was ranked number 3, followed by 3Y x NC (2,83 t/ha), which was ranked number 4. Twenty-two yellow QPM inbred lines were evaluated for agro-morphological characteristics. A randomized complete block design with three replicates was used under acidic and normal soil conditions at two locations. The mean performance of inbred lines for grain yield ranged from 1.6 t/ha to 3.1 t/ha under optimum conditions, whereas it ranged from 1.1.t/ha to 1.7 t/ha under an acidic environment. The highest grain yielding genotypes under the optimum environment were 14Y, 7Y, and QSY5, while, in the acidic environment, the highest yielders were 3Y, QSY5, and 17Y. The lowest grain yielder under the optimum environment was 16Y at 1.5 t/ha, whereas 6Y, 7Y, 8Y, and 29Y were the most moderate yielders under the acidic environment. Grain yield displayed positive and significant (p<0,05) correlation with ear height (0.59), plant height (0.55), leaf length (0.18), number of leaves (0.37), number of ears per plot (0,54), number of kernels per row, number of rows per ear and thousand kernel weight. Combined ANOVA revealed that the environment effect was significant for all traits except the number of rows per ear and the number of ears in both environments. A total of 71 genotypes (22 inbred lines, forty-four single crosses, and five checks) were evaluated using a 7 x 7 alpha lattice design with two replications in combining ability. Across environments, the analysis showed a mean grain yield record of hybrids that ranged from 1.78 t/ha to 3.42 t/ha. Combined analysis showed only about 4 percent of the 44 hybrids had better grain yield than hybrid checks, whereas about 11 percent of the 44 hybrids had higher grain yield than two of the five hybrid checks. The highest yielding hybrid was 14Y x NC followed by QSY5 x NC. The Mid-parent heterosis (MPH) for grain yield ranged from -32.70 for 11YxObat to 16.50 percent for 14YxNC among the top-yielding genotypes. The highest positive mid-parent heterosis (MPH) for grain yield was observed in hybrid 14YxNC followed by QSY9xNC, QSY5xOBAT, 3YxNC, 13YxNC and 4YxOBAT, while the remaining hybrids exhibited negative estimates. Inbred lines' proportional contributions to the total variances were much higher than the testers for all traits. Whereas the inbred lines' proportional contribution was lower than the interaction of line x tester except on the number of ears which displayed the highest estimates of variances due to SCA effects, significant variation was observed. The GCA effects on grain yield in both environments generally showed no significant difference among inbred lines except for inbred lines such as QSY5 and 34Y but were positive. The selected crosses showed significant SCA effects in a few agronomic yield-related traits in the combined analysis. Among the hybrids, the only crosses that showed significant and positive SCA effects for grain yield were 25YxNC under optimum and 34YxObat under acidic environment. The inbred lines were assessed for tolerance to Al toxicity under laboratory conditions. Overall, the study identified soil acidity tolerant QPM inbred lines with an excellent combining ability for high-yielding QPM test hybrids, grain yield, and related traits. , Thesis (MSc) (Agriculture, Crop Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Bobotyana, Simthandile
- Date: 2021-04
- Subjects: Corn as food , Plants--Drought tolerance
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20528 , vital:46087
- Description: Maize (Zea mays L) is an essential food grain crop in South Africa and is mainly grown under rainfed conditions by small-scale farmers. Quality Protein Maize (QPM) contains a higher level of two essential amino acids, tryptophan, and lysine when equated to normal maize. Therefore, the QPM in communities is a vital cereal where maize is a staple crop. Among the significant abiotic stresses that adversely affect maize productivity, such as soil fertility. Low soil pH or soil acidity results in nutrient deficiencies, as well as the toxicity of minerals such as manganese (Mn), aluminium (Al), and iron (Fe). The objectives of the study were to: (1) Characterize yellow QPM inbred lines and testcross hybrids for agro-morphological variability under optimum conditions, (2) Determine the response of QPM inbred lines and testcross hybrids to Al toxicity, and (3) Estimate the combining ability of QPM genotypes for the tolerance to Al toxicity. The laboratory assessment for tolerance to Al toxicity revealed a negative relationship between relative root growth RRG and hematoxylin staining; among the inbred lines, the genotype 8Y, 17Y, and 7Y had the highest RRG and net root growth (NRG) values and exhibited the highest tolerance to Al tolerance with the least hematoxylin staining. Inbred lines 12Y, 16Y, 19Y, 2Y, 20Y, 24Y, 30Y, 27Y, 34Y, 4Y, and 11Y were moderately tolerant. On the other hand, inbred lines 13Y, 18Y, and QSY5 had the least RRG were susceptible. Forty-four testcross hybrids were generated by crossing 22 QPM inbred lines with two open-pollinated variety (OPV) testers. These hybrids were evaluated together with five checks for different agronomic traits. The hybrids were planted in the summer season of 2017/18 and 2018/19 under rain-fed conditions 7 x 7 alpha lattice design was used. The significant variations among the genotypes for grain yield and most other agronomic traits under optimum, acidic conditions and across environments on combined analysis of variance. The hybrid was 14Y x NC (3.22 t/ha), followed by QSY5 x NC (3.02 t/ha) showed the highest yielding. The mean plant height was 226.23 cm, with ranges 197.60 to 254.85cm. The number of kernels per ear was 35, with a range from 29.45 to 40.25. Conversely, the mean ear length was 19.30 cm with a range of 17.65 to 20.95 cm, and the mean ear diameter ranged from 13,50 to 16,05 cm. The highest yielding hybrid, according to ranking, was 14Y x NC (2,93t/ha), which was ranked number 3, followed by 3Y x NC (2,83 t/ha), which was ranked number 4. Twenty-two yellow QPM inbred lines were evaluated for agro-morphological characteristics. A randomized complete block design with three replicates was used under acidic and normal soil conditions at two locations. The mean performance of inbred lines for grain yield ranged from 1.6 t/ha to 3.1 t/ha under optimum conditions, whereas it ranged from 1.1.t/ha to 1.7 t/ha under an acidic environment. The highest grain yielding genotypes under the optimum environment were 14Y, 7Y, and QSY5, while, in the acidic environment, the highest yielders were 3Y, QSY5, and 17Y. The lowest grain yielder under the optimum environment was 16Y at 1.5 t/ha, whereas 6Y, 7Y, 8Y, and 29Y were the most moderate yielders under the acidic environment. Grain yield displayed positive and significant (p<0,05) correlation with ear height (0.59), plant height (0.55), leaf length (0.18), number of leaves (0.37), number of ears per plot (0,54), number of kernels per row, number of rows per ear and thousand kernel weight. Combined ANOVA revealed that the environment effect was significant for all traits except the number of rows per ear and the number of ears in both environments. A total of 71 genotypes (22 inbred lines, forty-four single crosses, and five checks) were evaluated using a 7 x 7 alpha lattice design with two replications in combining ability. Across environments, the analysis showed a mean grain yield record of hybrids that ranged from 1.78 t/ha to 3.42 t/ha. Combined analysis showed only about 4 percent of the 44 hybrids had better grain yield than hybrid checks, whereas about 11 percent of the 44 hybrids had higher grain yield than two of the five hybrid checks. The highest yielding hybrid was 14Y x NC followed by QSY5 x NC. The Mid-parent heterosis (MPH) for grain yield ranged from -32.70 for 11YxObat to 16.50 percent for 14YxNC among the top-yielding genotypes. The highest positive mid-parent heterosis (MPH) for grain yield was observed in hybrid 14YxNC followed by QSY9xNC, QSY5xOBAT, 3YxNC, 13YxNC and 4YxOBAT, while the remaining hybrids exhibited negative estimates. Inbred lines' proportional contributions to the total variances were much higher than the testers for all traits. Whereas the inbred lines' proportional contribution was lower than the interaction of line x tester except on the number of ears which displayed the highest estimates of variances due to SCA effects, significant variation was observed. The GCA effects on grain yield in both environments generally showed no significant difference among inbred lines except for inbred lines such as QSY5 and 34Y but were positive. The selected crosses showed significant SCA effects in a few agronomic yield-related traits in the combined analysis. Among the hybrids, the only crosses that showed significant and positive SCA effects for grain yield were 25YxNC under optimum and 34YxObat under acidic environment. The inbred lines were assessed for tolerance to Al toxicity under laboratory conditions. Overall, the study identified soil acidity tolerant QPM inbred lines with an excellent combining ability for high-yielding QPM test hybrids, grain yield, and related traits. , Thesis (MSc) (Agriculture, Crop Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-04
Bio-utilization of keratinous waste biomass for the production of keratinolytic proteases by Chryseobactreium aquifrigidense isolated from poultry waste dumpsite
- Authors: Bokveld, Amahle
- Date: 2021-02
- Subjects: Keratin
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20550 , vital:46120
- Description: Keratin is an insoluble and a fibrous protein that is mostly found in feathers, animal wool, and hair, making them mechanically stable. Avian feathers are the most ubiquitously generated keratinous waste biomass from the poultry processing plants. Keratinous waste biomass valorization could produce amino acids and bioactive peptides. Hence, the bio-recycling of keratin-rich wastes bears an advantage over the chemical and thermal means. In recent times, the microbial keratinases continues to gain traction due to the litany of their potential significance in green technology. Consequently, this study assessed chicken feather degrading and keratinase production potentials of bacteria isolated from a local poultry dumpsite. Soil samples were collected from the poultry dumpsite. Bacteria were isolated using basal salt medium and screened for keratinolytic activity. The identification of potent chicken feather degrading bacterial isolates was through 16S rRNA gene sequence analysis. Keratinase production efficiency of isolates on chicken feather constituted medium was optimized. Hydrolysate's amino acid compositions were quantified, and the keratinases produced was characterized. Out of 22 bacteria isolated from the soil samples, 12 showed a varying degree of proteolytic activity on skimmed milk agar. Four (4) isolates, coded as FPS-01, FPS-07, FPS-09, WDS-06, out of the 12 proteolytic strains further displayed remarkable degradation of the intact chicken feather; percentage degradation ranged from 69 percent for FPS-01 to 88 percent for FPS-09. The extracellular keratinase activity ranged from 610.91 ± 56.57 U/mL for WDS-06 and 834.55 ± 66.86 U/mL for FPS-07. Additionally, the sulfhydryl group concentration quantified from the fermentation broth was 2.22 ± 0.37 (mM), 0.22 ± 0.08 (mM), 2.27 ± 0.09 (mM), and 2.76 ± 0.79 (mM) for FPS-01, FPS-07, FPS-09 and WDS-06, respectively. Based on 16S rRNA gene sequencing and phylogenetic analyses, the isolates FPS-07 and FPS-09 were identified as Chryseobacterium aquifrigidense FANN1 and Chryseobacterium aquifrigidense FANN2. Following the optimization process, the improved fermentation conditions were pH 6, inoculum side (4 percent, v/v), temperature (30 oC), and chicken feather (0.5-1.5 percent, w/v) for FANN1. FANN2 optimal fermentation conditions were pH 6, inoculum (5 percent, v/v), temperature (30 oC), and chicken feather (1.5 percent, w/v). Both bacterial isolates showed the highest extracellular keratinase production after 72 h of the fermentation time. Analysis of the hydrolysates generated from the bacteria fermentation showed a high concentration of arginine, serine, glutamic acid, glycine, proline, valine, and leucine at a respective concentration of 1.13, 1.02, 0.83, 0.94, 0.85, 0.84, and 0.86 (g/100g sample) against FANN1. Similarly, FANN2 generated hydrolysate showed high concentrations of glutamic acid, arginine, serine, aspartic acid, and glycine at this respective abundance 2.52, 1.92, 2.12, 2.25 and 1.9 (g/100g sample). Keratinases from FANN1 and FANN2 showed optimal catalytic efficiency at pH 8 and temperature between 40-50 oC. The enzyme was considerably thermostable at 40 oC and 50 oC after 120 min of preheating. Both FANN1 and FANN2 showed variable residual activity in the presence of the different metal ions. Keratinase from FANN1 recorded the following residual activity of Fe3+ (120 ± 5.06 percent), Ca2+ (100 ± 10.33 percent), Na+ (122 ± 2.95 percent), Al3+ (106 ± 10.33 percent). Likewise, FANN2 keratinase showed remarkable stability against Na+ (108 ± 13.71 percent), Ba2+ (102 ± 0.86 percent), Al3+ (105 ± 2.57 percent), and Ca2+ (96 ± 2.99 percent). Keratinase from FANN1 was catalytically activated after 60 min of pre-treatment with the following detergents, Sunlight (129 percent), Ariel (116 percent), MAQ (151 percent), and Surf (143 percent) compared to the control. FANN2 keratinase showed less stability with laundry detergents after 60 min of preincubation. FANN1 keratinase showed remarkable stability in the presence of chemical agents tested, with residual activity of 90 ± 0.18 percent, 105 ± 7.55 percent, 108 ± 4.31 percent, 123 ± 1.44 percent, 132 ± 1.26 percent, 96 ± 7.19 percent, and 101 ± 3.06 percent for DTT, hydrogen peroxide, DMSO, acetonitrile, triton X-100, tween-80, and SDS, respectively. The enzyme activity was also considerably inhibited by PMSF and EDTA, which suggested a mixed type of protease. Furthermore, keratinase from FANN2 was inhibited by EDTA, and such inhibition pattern grouped it as a metallo-type of protease. The enzyme was also stable in the presence of other chemical agents tested. Therefore, the findings suggest the isolates and their enzymes' relevance to sustainable recycling of recalcitrant keratinous wastes into high-value products with immense application potentials. The remarkable stability shown by keratinases from FANN1 and FANN2, post detergent and chemical agents pre-treatment, indicates promise for the biotechnology and industrial sector. , Thesis(MSc) (Microbiology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Bokveld, Amahle
- Date: 2021-02
- Subjects: Keratin
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20550 , vital:46120
- Description: Keratin is an insoluble and a fibrous protein that is mostly found in feathers, animal wool, and hair, making them mechanically stable. Avian feathers are the most ubiquitously generated keratinous waste biomass from the poultry processing plants. Keratinous waste biomass valorization could produce amino acids and bioactive peptides. Hence, the bio-recycling of keratin-rich wastes bears an advantage over the chemical and thermal means. In recent times, the microbial keratinases continues to gain traction due to the litany of their potential significance in green technology. Consequently, this study assessed chicken feather degrading and keratinase production potentials of bacteria isolated from a local poultry dumpsite. Soil samples were collected from the poultry dumpsite. Bacteria were isolated using basal salt medium and screened for keratinolytic activity. The identification of potent chicken feather degrading bacterial isolates was through 16S rRNA gene sequence analysis. Keratinase production efficiency of isolates on chicken feather constituted medium was optimized. Hydrolysate's amino acid compositions were quantified, and the keratinases produced was characterized. Out of 22 bacteria isolated from the soil samples, 12 showed a varying degree of proteolytic activity on skimmed milk agar. Four (4) isolates, coded as FPS-01, FPS-07, FPS-09, WDS-06, out of the 12 proteolytic strains further displayed remarkable degradation of the intact chicken feather; percentage degradation ranged from 69 percent for FPS-01 to 88 percent for FPS-09. The extracellular keratinase activity ranged from 610.91 ± 56.57 U/mL for WDS-06 and 834.55 ± 66.86 U/mL for FPS-07. Additionally, the sulfhydryl group concentration quantified from the fermentation broth was 2.22 ± 0.37 (mM), 0.22 ± 0.08 (mM), 2.27 ± 0.09 (mM), and 2.76 ± 0.79 (mM) for FPS-01, FPS-07, FPS-09 and WDS-06, respectively. Based on 16S rRNA gene sequencing and phylogenetic analyses, the isolates FPS-07 and FPS-09 were identified as Chryseobacterium aquifrigidense FANN1 and Chryseobacterium aquifrigidense FANN2. Following the optimization process, the improved fermentation conditions were pH 6, inoculum side (4 percent, v/v), temperature (30 oC), and chicken feather (0.5-1.5 percent, w/v) for FANN1. FANN2 optimal fermentation conditions were pH 6, inoculum (5 percent, v/v), temperature (30 oC), and chicken feather (1.5 percent, w/v). Both bacterial isolates showed the highest extracellular keratinase production after 72 h of the fermentation time. Analysis of the hydrolysates generated from the bacteria fermentation showed a high concentration of arginine, serine, glutamic acid, glycine, proline, valine, and leucine at a respective concentration of 1.13, 1.02, 0.83, 0.94, 0.85, 0.84, and 0.86 (g/100g sample) against FANN1. Similarly, FANN2 generated hydrolysate showed high concentrations of glutamic acid, arginine, serine, aspartic acid, and glycine at this respective abundance 2.52, 1.92, 2.12, 2.25 and 1.9 (g/100g sample). Keratinases from FANN1 and FANN2 showed optimal catalytic efficiency at pH 8 and temperature between 40-50 oC. The enzyme was considerably thermostable at 40 oC and 50 oC after 120 min of preheating. Both FANN1 and FANN2 showed variable residual activity in the presence of the different metal ions. Keratinase from FANN1 recorded the following residual activity of Fe3+ (120 ± 5.06 percent), Ca2+ (100 ± 10.33 percent), Na+ (122 ± 2.95 percent), Al3+ (106 ± 10.33 percent). Likewise, FANN2 keratinase showed remarkable stability against Na+ (108 ± 13.71 percent), Ba2+ (102 ± 0.86 percent), Al3+ (105 ± 2.57 percent), and Ca2+ (96 ± 2.99 percent). Keratinase from FANN1 was catalytically activated after 60 min of pre-treatment with the following detergents, Sunlight (129 percent), Ariel (116 percent), MAQ (151 percent), and Surf (143 percent) compared to the control. FANN2 keratinase showed less stability with laundry detergents after 60 min of preincubation. FANN1 keratinase showed remarkable stability in the presence of chemical agents tested, with residual activity of 90 ± 0.18 percent, 105 ± 7.55 percent, 108 ± 4.31 percent, 123 ± 1.44 percent, 132 ± 1.26 percent, 96 ± 7.19 percent, and 101 ± 3.06 percent for DTT, hydrogen peroxide, DMSO, acetonitrile, triton X-100, tween-80, and SDS, respectively. The enzyme activity was also considerably inhibited by PMSF and EDTA, which suggested a mixed type of protease. Furthermore, keratinase from FANN2 was inhibited by EDTA, and such inhibition pattern grouped it as a metallo-type of protease. The enzyme was also stable in the presence of other chemical agents tested. Therefore, the findings suggest the isolates and their enzymes' relevance to sustainable recycling of recalcitrant keratinous wastes into high-value products with immense application potentials. The remarkable stability shown by keratinases from FANN1 and FANN2, post detergent and chemical agents pre-treatment, indicates promise for the biotechnology and industrial sector. , Thesis(MSc) (Microbiology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
Morphodynamics of headland bypass dunefields, with special reference to the Cape St Francis headland, Eastern Cape, South Africa
- Authors: Burkinshaw, Jennifer Ruth
- Date: 1998-01
- Subjects: Sand dunes -- South Africa -- Eastern Cape -- Cape St Francis
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/52814 , vital:44206
- Description: The temperate Cape south coast, South Africa, is characterised by a series of log-spiral bays bound by low-relief rocky headlands. In South Africa corridors of transverse dunes crossing the headlands are referred to as headland bypass dunefields (HBDs). Sand blown off upwind beaches is transported across the headlands towards the downwind bays, bypassing the littoral route around the headlands. The key to the development of the HBDs is the presence of sandy beaches upwind of the headlands which lie athwart the prevailing winds. The low relief of the headlands and their shoreline configuration, coupled with their exposure to the marine wind regime, are such that the predominant high energy, westerly winds blow directly onshore. The local longshore drift system plays a critical role in replenishing beach sand deflated by the wind. The "type" HBDs occur in the Eastern Cape on the prominent Cape St Francis and Cape Recife headlands where HBDs have been active since at least the Mid to Late Pleistocene. The dunefields have length/width ratios ranging from 3 to over 10. Sand coverage within HBDs is metasaturated, i.e., the transverse dunes are separated by well-defined interdune areas. Average dune height in the larger dunefields in the Eastern Cape is 9-10 m. The HBDs are commonly bound by sidewalls that develop along the interface between the dunefields and vegetation. The stabilised sidewalls provide the most prominent record of former HBD activity and can influence subsequent HBD morphology. The Oyster Bay dunefield on the Cape St Francis headland was chosen for a study of the aeolian morphodynamic processes that operate within these systems. The dunefield currently operates over a distance of about 14 km and a width of 500 - 1200 m; it is at present cut off from its sand source. Shallow lakes develop in the interdune areas after periods of high rainfall. A 2 week airflow study recorded wind speed along the prevailing wind axis of the Oyster Bay dunefield during spring 1990. Wind speed increased significantly downstream until at least the mid-dunefield area during both the prevailing westerly and seasonal easterly winds. The downwind wind speed increase can be partially attributed to topographic acceleration of airflow as it is compressed over the low cape. The extent of the wind speed increase can vary depending on the land/sea temperature contrasts and the temperature structure of the lower atmosphere. Wind speed recorded across the width of the dunefield was slowed down along the margins by vegetation, while a faster core moved down the centre of the dunefield. Rates of dune movement along the prevailing wind axis show a general correspondence between the volume of sand transported eastwards in different areas of the dunefield and the airflow pattern down the length of the dunefield. Sand is transported most efficiently in the centre of the dunefield where westerly flow is at its maximum. Sand transport calculated from dune migration rates is about 50% of potential sand transport calculated from wind data, as flow is metasaturated. Flow off sandy beaches may be naturally below saturation owing to the effect of intertidal moisture and salts binding the sand. In the Eastern Cape metasaturation can be further enhanced by the downwind entrapment of sand by vegetation along the dunefield margins and within wet interdune areas. The 2-dimensional model for bedform development within HBDs focuses on the downwind extension of the dunefields and transmission of sand through the systems. The combination of the high transport capacity of the wind downwind and metasaturated flow maintains the transport of sand across headlands. HBDs operate aerodynamically as erosional or sediment bypassing systems although deflation of the wet interdune areas is limited. Fully functional short HBDs are the most likely to operate with neutral sediment budgets. Long systems are more susceptible to spatial and temporal effects that modify the sandflux through the systems and hence the outflux from the systems. The Oyster Bay dunefield operates with a mildly positive sediment budget because of sediment entrapment downwind. The morphology and morphodynamic behaviour of HBDs is in contrast to that of the tabular transgressive dunefields that form parallel to the shores of the large log-spiral bays of Algoa Bay and St Francis Bay. The existing morphological classification of transgressive dunefields needs to be revised to include headland bypass dunefields. Keywords: headland bypass dunefields, transgressive coastal dunefields, aeolian morphodynamics, Cape St Francis, Cape south coast. , Thesis (PhD) -- Faculty of Science, 1998
- Full Text:
- Date Issued: 1998-01
- Authors: Burkinshaw, Jennifer Ruth
- Date: 1998-01
- Subjects: Sand dunes -- South Africa -- Eastern Cape -- Cape St Francis
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/52814 , vital:44206
- Description: The temperate Cape south coast, South Africa, is characterised by a series of log-spiral bays bound by low-relief rocky headlands. In South Africa corridors of transverse dunes crossing the headlands are referred to as headland bypass dunefields (HBDs). Sand blown off upwind beaches is transported across the headlands towards the downwind bays, bypassing the littoral route around the headlands. The key to the development of the HBDs is the presence of sandy beaches upwind of the headlands which lie athwart the prevailing winds. The low relief of the headlands and their shoreline configuration, coupled with their exposure to the marine wind regime, are such that the predominant high energy, westerly winds blow directly onshore. The local longshore drift system plays a critical role in replenishing beach sand deflated by the wind. The "type" HBDs occur in the Eastern Cape on the prominent Cape St Francis and Cape Recife headlands where HBDs have been active since at least the Mid to Late Pleistocene. The dunefields have length/width ratios ranging from 3 to over 10. Sand coverage within HBDs is metasaturated, i.e., the transverse dunes are separated by well-defined interdune areas. Average dune height in the larger dunefields in the Eastern Cape is 9-10 m. The HBDs are commonly bound by sidewalls that develop along the interface between the dunefields and vegetation. The stabilised sidewalls provide the most prominent record of former HBD activity and can influence subsequent HBD morphology. The Oyster Bay dunefield on the Cape St Francis headland was chosen for a study of the aeolian morphodynamic processes that operate within these systems. The dunefield currently operates over a distance of about 14 km and a width of 500 - 1200 m; it is at present cut off from its sand source. Shallow lakes develop in the interdune areas after periods of high rainfall. A 2 week airflow study recorded wind speed along the prevailing wind axis of the Oyster Bay dunefield during spring 1990. Wind speed increased significantly downstream until at least the mid-dunefield area during both the prevailing westerly and seasonal easterly winds. The downwind wind speed increase can be partially attributed to topographic acceleration of airflow as it is compressed over the low cape. The extent of the wind speed increase can vary depending on the land/sea temperature contrasts and the temperature structure of the lower atmosphere. Wind speed recorded across the width of the dunefield was slowed down along the margins by vegetation, while a faster core moved down the centre of the dunefield. Rates of dune movement along the prevailing wind axis show a general correspondence between the volume of sand transported eastwards in different areas of the dunefield and the airflow pattern down the length of the dunefield. Sand is transported most efficiently in the centre of the dunefield where westerly flow is at its maximum. Sand transport calculated from dune migration rates is about 50% of potential sand transport calculated from wind data, as flow is metasaturated. Flow off sandy beaches may be naturally below saturation owing to the effect of intertidal moisture and salts binding the sand. In the Eastern Cape metasaturation can be further enhanced by the downwind entrapment of sand by vegetation along the dunefield margins and within wet interdune areas. The 2-dimensional model for bedform development within HBDs focuses on the downwind extension of the dunefields and transmission of sand through the systems. The combination of the high transport capacity of the wind downwind and metasaturated flow maintains the transport of sand across headlands. HBDs operate aerodynamically as erosional or sediment bypassing systems although deflation of the wet interdune areas is limited. Fully functional short HBDs are the most likely to operate with neutral sediment budgets. Long systems are more susceptible to spatial and temporal effects that modify the sandflux through the systems and hence the outflux from the systems. The Oyster Bay dunefield operates with a mildly positive sediment budget because of sediment entrapment downwind. The morphology and morphodynamic behaviour of HBDs is in contrast to that of the tabular transgressive dunefields that form parallel to the shores of the large log-spiral bays of Algoa Bay and St Francis Bay. The existing morphological classification of transgressive dunefields needs to be revised to include headland bypass dunefields. Keywords: headland bypass dunefields, transgressive coastal dunefields, aeolian morphodynamics, Cape St Francis, Cape south coast. , Thesis (PhD) -- Faculty of Science, 1998
- Full Text:
- Date Issued: 1998-01
The role of national culture characteristics and business orientations in Tanzanian small business success
- Authors: Chachage, Bukaza Loth
- Date: 2021-04
- Subjects: Success in business -- Tanzania , Small business -- Tanzania , New business enterprises -- Tanzania
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/51061 , vital:43205
- Description: Despite numerous efforts to increase the business success of Tanzanian small businesses, it is still reported that progress in this regard has stagnated. The desired success in the SMME sector, and small businesses, is not being achieved, and is one of the reasons poverty in Tanzania remains significantly high. Researchers have suggested that low-income countries (LICs) such as Tanzania often do not achieve the desired growth of business enterprises due to insufficient attention being paid to issues of national culture and business orientations. Against this background, the purpose of the current study is to contribute to small business success in Tanzania by investigating the role that national culture characteristics (egalitarianism, mastery, harmony, embeddedness, affective autonomy and intellectual autonomy) and business orientations (technology, market and entrepreneurial) play in increasing the business success of Tanzanian small businesses. In this study, business success is defined as the extent to which small businesses effectively manage marketing, operations, human resources and finances. Located in the quantitative research paradigm, and using a mail survey data collection method, a sample of 640 SMMEs was targeted from eight regional economic zones in Tanzania. Of this group, 410 SMMEs, mostly small businesses, participated in the study (response rate = 64%). Instruments based on the works of Burgess (2011) and Schwartz (2004) were used to measure the business orientations and national culture characteristics of the selected Tanzanian small business owners and managers. The instrument developed by the South African Small Enterprise Development Agency (SEDA 2004) was used to measure small business success. The data analyses in this study included tests for data normalcy and the reliability and validity of the data (using Cronbach ‘s alpha and confirmatory factor analysis, respectively). Furthermore, multiple regression analyses were conducted to investigate the hypothesised relationships and other research propositions. The results of this study provided sound empirical proof of the interactive influence of national culture characteristics and business orientations on the business success of small businesses. Based on these empirical findings, a proposed framework for small business success in Tanzania was developed. The empirical results of the study provided evidence that support several existing theories, perceptions and beliefs about the relationship between national culture characteristics and business orientations. Certain findings also provided evidence that challenged aspects of existing theories, perceptions and beliefs. The study specifically highlights the importance of the technology and entrepreneurial business orientations as key positive determinants of the effectiveness of all four business functions (marketing, operations, human resources and financial management) investigated in the study. A surprising finding was the significantly negative relationship between market orientation and human resources management. The study also highlighted the centrality of the national characteristics of egalitarianism and intellectual autonomy in the development of the three business orientations (technology, entrepreneurial and market) investigated in the study. The findings suggest that the principle of the equality of people and a commitment to the continuous search and rewarding of new ideas and unique ways of doing things through continuous and professional development are important drivers of critical business orientations among Tanzanian small business owners and managers. The empirical findings furthermore highlighted the importance of mastery as a national culture characteristic. Mastery, which emphasises competence, goal-setting, hard-work, efficiency, effectiveness and self-reliance in attaining goals, influenced the effectiveness of all four business functions and two of the three business orientations investigated in the study. Finally, the study made significant contributions to the understanding of small business success in Tanzania and, arguably, across the African continent. The infusion of these insights into strategies for entrepreneurship development will go a long way to building successful businesses in the small business sector. , Thesis (DBA) -- Faculty of Business and Economic Sciences, Business Administration, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Chachage, Bukaza Loth
- Date: 2021-04
- Subjects: Success in business -- Tanzania , Small business -- Tanzania , New business enterprises -- Tanzania
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/51061 , vital:43205
- Description: Despite numerous efforts to increase the business success of Tanzanian small businesses, it is still reported that progress in this regard has stagnated. The desired success in the SMME sector, and small businesses, is not being achieved, and is one of the reasons poverty in Tanzania remains significantly high. Researchers have suggested that low-income countries (LICs) such as Tanzania often do not achieve the desired growth of business enterprises due to insufficient attention being paid to issues of national culture and business orientations. Against this background, the purpose of the current study is to contribute to small business success in Tanzania by investigating the role that national culture characteristics (egalitarianism, mastery, harmony, embeddedness, affective autonomy and intellectual autonomy) and business orientations (technology, market and entrepreneurial) play in increasing the business success of Tanzanian small businesses. In this study, business success is defined as the extent to which small businesses effectively manage marketing, operations, human resources and finances. Located in the quantitative research paradigm, and using a mail survey data collection method, a sample of 640 SMMEs was targeted from eight regional economic zones in Tanzania. Of this group, 410 SMMEs, mostly small businesses, participated in the study (response rate = 64%). Instruments based on the works of Burgess (2011) and Schwartz (2004) were used to measure the business orientations and national culture characteristics of the selected Tanzanian small business owners and managers. The instrument developed by the South African Small Enterprise Development Agency (SEDA 2004) was used to measure small business success. The data analyses in this study included tests for data normalcy and the reliability and validity of the data (using Cronbach ‘s alpha and confirmatory factor analysis, respectively). Furthermore, multiple regression analyses were conducted to investigate the hypothesised relationships and other research propositions. The results of this study provided sound empirical proof of the interactive influence of national culture characteristics and business orientations on the business success of small businesses. Based on these empirical findings, a proposed framework for small business success in Tanzania was developed. The empirical results of the study provided evidence that support several existing theories, perceptions and beliefs about the relationship between national culture characteristics and business orientations. Certain findings also provided evidence that challenged aspects of existing theories, perceptions and beliefs. The study specifically highlights the importance of the technology and entrepreneurial business orientations as key positive determinants of the effectiveness of all four business functions (marketing, operations, human resources and financial management) investigated in the study. A surprising finding was the significantly negative relationship between market orientation and human resources management. The study also highlighted the centrality of the national characteristics of egalitarianism and intellectual autonomy in the development of the three business orientations (technology, entrepreneurial and market) investigated in the study. The findings suggest that the principle of the equality of people and a commitment to the continuous search and rewarding of new ideas and unique ways of doing things through continuous and professional development are important drivers of critical business orientations among Tanzanian small business owners and managers. The empirical findings furthermore highlighted the importance of mastery as a national culture characteristic. Mastery, which emphasises competence, goal-setting, hard-work, efficiency, effectiveness and self-reliance in attaining goals, influenced the effectiveness of all four business functions and two of the three business orientations investigated in the study. Finally, the study made significant contributions to the understanding of small business success in Tanzania and, arguably, across the African continent. The infusion of these insights into strategies for entrepreneurship development will go a long way to building successful businesses in the small business sector. , Thesis (DBA) -- Faculty of Business and Economic Sciences, Business Administration, 2021
- Full Text:
- Date Issued: 2021-04
Knowledge and attitude towards mastitis, culling and mortality among dairy farmworkers, and factors associated with culling and mortality among dairy cattle in the Eastern Cape Province, South Africa
- Authors: Diniso, Yanga Simamkele
- Date: 2021-09
- Subjects: Food security , Dairying--Economic aspects , Milking
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20686 , vital:46425
- Description: Globally, food security is under threat from escalating population growth, new variants of diseases, climate change and livestock loss. Africa is vulnerable to food insecurity, with majority of the estimated 800 million suffering from hunger based in Africa. Intensification of production is a common strategy to counter the increasing demand for nutritional food to maintain and improve food security. The dairy industry is one of the leading agriculture sectors that has adopted intensification of production. The current study had two specific objectives; the first objective was to evaluate the dairy farmworkers’ knowledge and attitude towards mastitis, culling, and mortality in the southern part of the Eastern Cape Province. The data was collected from 106 dairy farm workers of different farm hierarchy in 5 out of 6 districts using questionnaire. In cases whereby a majority correctly answered a question, a point was awarded and a zero for incorrectly answered questions by the majority. A less than 50 percent pass rate was considered a poor level of knowledge, 51-69 percent pass rate was considered an average level of knowledge, and anything higher than that considered a good level of knowledge. The majority of the participants (64.2 percent) indicated that they consume unpasteurised milk even though they know its risks. There was no association (P>0.05) between workplace position, educational level, work experience, and consumption of unpasteurised milk. This study found that participants had a deficient level of knowledge of mastitis causing pathogens by attaining a pass mark of 1.60 out of a possible 10 (16 percent), and educational level was significantly associated with knowledge of Escherichia coli, Staphylococcus aureus, and Streptococcus spp. However, the participants (64.9 percent) showed good knowledge of clinical signs of mastitis, and this was attributed to the regular mastitis detection with California Mastitis Test (CMT, 60.4 percent) and a strip cup (83.0 percent). The participants had varying perceptions and limited knowledge (28.3 percent pass rate) about the major contributing factors of culling and mortality. The lack of dairy training courses and minimal use of other sources of information such as the internet might contribute to this poor knowledge and perceptions. The second objective involved identifying and describing the factors responsible for culling and mortality in dairy farms in the Eastern Cape province, South Africa. The factors were grouped into seven (7) major factors namely lameness and poor growth (2.33 percent), reproduction problems (35.86 percent), health factors (6.72 percent), production factors (7.11 percent), udder-related factors (3.42 percent), voluntary culls (4.86 percent) and miscellaneous factors (26.03 percent). The most frequent reason for culling dairy cows was reproduction problems which was mainly made up of infertility (7.9 percent), reproductive failure (89.9 percent), and dystocia (1 percent). Miscellaneous factors which mainly consisted of indigenous breeds (8.2 percent), and age (2.7 percent) accounted for the second most prevalent factors for the culling of dairy cows. All the farms recorded stock-theft as part of involuntary culling and accounted for 3.6 percent dairy cows that were culled. Poor milk yield and health-related factors such as red-water (33.8 percent), milk-fever (23.3 percent), and heart-water (6.8 percent) were amongst the major factors responsible for the culling of dairy cows. There were 1774 (13.67 percent) dairy cows that died in the study farms from 2015 to 2019 and causes of death were not specified except for drowning (0.01 percent) and snake bite (1 percent). This study exposed a gap in knowledge about the causes of mastitis, culling and mortality among dairy farmworkers in the southern part of the Eastern Cape Province. Furthermore, this study provided with a baseline information on the leading causes of culling and mortality. There is a need for further research on strategies of enhancing the knowledge of dairy farmworkers about pathogens of mastitis and specific causes of culling and mortality to mitigate the challenge they pose to food security. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Diniso, Yanga Simamkele
- Date: 2021-09
- Subjects: Food security , Dairying--Economic aspects , Milking
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20686 , vital:46425
- Description: Globally, food security is under threat from escalating population growth, new variants of diseases, climate change and livestock loss. Africa is vulnerable to food insecurity, with majority of the estimated 800 million suffering from hunger based in Africa. Intensification of production is a common strategy to counter the increasing demand for nutritional food to maintain and improve food security. The dairy industry is one of the leading agriculture sectors that has adopted intensification of production. The current study had two specific objectives; the first objective was to evaluate the dairy farmworkers’ knowledge and attitude towards mastitis, culling, and mortality in the southern part of the Eastern Cape Province. The data was collected from 106 dairy farm workers of different farm hierarchy in 5 out of 6 districts using questionnaire. In cases whereby a majority correctly answered a question, a point was awarded and a zero for incorrectly answered questions by the majority. A less than 50 percent pass rate was considered a poor level of knowledge, 51-69 percent pass rate was considered an average level of knowledge, and anything higher than that considered a good level of knowledge. The majority of the participants (64.2 percent) indicated that they consume unpasteurised milk even though they know its risks. There was no association (P>0.05) between workplace position, educational level, work experience, and consumption of unpasteurised milk. This study found that participants had a deficient level of knowledge of mastitis causing pathogens by attaining a pass mark of 1.60 out of a possible 10 (16 percent), and educational level was significantly associated with knowledge of Escherichia coli, Staphylococcus aureus, and Streptococcus spp. However, the participants (64.9 percent) showed good knowledge of clinical signs of mastitis, and this was attributed to the regular mastitis detection with California Mastitis Test (CMT, 60.4 percent) and a strip cup (83.0 percent). The participants had varying perceptions and limited knowledge (28.3 percent pass rate) about the major contributing factors of culling and mortality. The lack of dairy training courses and minimal use of other sources of information such as the internet might contribute to this poor knowledge and perceptions. The second objective involved identifying and describing the factors responsible for culling and mortality in dairy farms in the Eastern Cape province, South Africa. The factors were grouped into seven (7) major factors namely lameness and poor growth (2.33 percent), reproduction problems (35.86 percent), health factors (6.72 percent), production factors (7.11 percent), udder-related factors (3.42 percent), voluntary culls (4.86 percent) and miscellaneous factors (26.03 percent). The most frequent reason for culling dairy cows was reproduction problems which was mainly made up of infertility (7.9 percent), reproductive failure (89.9 percent), and dystocia (1 percent). Miscellaneous factors which mainly consisted of indigenous breeds (8.2 percent), and age (2.7 percent) accounted for the second most prevalent factors for the culling of dairy cows. All the farms recorded stock-theft as part of involuntary culling and accounted for 3.6 percent dairy cows that were culled. Poor milk yield and health-related factors such as red-water (33.8 percent), milk-fever (23.3 percent), and heart-water (6.8 percent) were amongst the major factors responsible for the culling of dairy cows. There were 1774 (13.67 percent) dairy cows that died in the study farms from 2015 to 2019 and causes of death were not specified except for drowning (0.01 percent) and snake bite (1 percent). This study exposed a gap in knowledge about the causes of mastitis, culling and mortality among dairy farmworkers in the southern part of the Eastern Cape Province. Furthermore, this study provided with a baseline information on the leading causes of culling and mortality. There is a need for further research on strategies of enhancing the knowledge of dairy farmworkers about pathogens of mastitis and specific causes of culling and mortality to mitigate the challenge they pose to food security. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Factors influencing the adoption of mobile health monitoring and care systems by the elderly living at home in South Africa: a case of Buffalo City Metropolitan Municipality.
- Authors: Fotoyi, Yolande Odwa
- Date: 2021-09
- Subjects: Medical informatics , Wireless communication systems in medical care
- Language: English
- Type: Master's/ theses , text
- Identifier: http://hdl.handle.net/10353/20406 , vital:45662
- Description: The gradual increase of elderly people around the globe necessitates intensive dialogue amongst government, the healthcare sector and elderly communities as per MPAA 2002 resolutions. Literature identifies technology as the enabler to drive the facilitation of improved living conditions beginning with an affordable, accessible and integrated health information system (HIS). The attainment of a better quality of care to meet the elderly’s needs requires the re-engineering of current modalities. The diverse nature of South Africa is more suited to a people-based rather than a process-centric approach currently in existence. Access barriers, affordability, the digital divide, lack of government buy-in, and fragmented HIS are considered major impediments to adoption of mobile monitoring and care systems (MMCs) for the elderly’s healthcare. Given the complications brought about by the Covid-19 pandemic, the adoption of MMCs cannot be more pronounced. However, despite available literature regarding elderly issues in both developed and developing countries, the elderly plight has still not been considered a national priority. The main purpose of this research was to investigate why elderly people do not adopt MMCs to improve their quality of life, with MMC technologies as a general area of research. The main objective of the study was to develop critical success factors to improve the adoption of MMCs by the elderly living at home. This would potentially alleviate the burden on healthcare resources and also improve the elderly’s quality of life. Primary data collection took place from 21 February to 28 February 2020 in Buffalo City Metropolitan Municipality. Semi-structured interviews were conducted with 15 participants comprising one male and 14 females who represented the elderly Black, Coloured, Indian and White people. This qualitative research tool and purposive sampling method were chosen in order to fully capture the participants’ experiences in the home environment, which excluded those living in frail care or step-down facilities or state institutions. Despite the sample size being small and not being generalizable, it delivered rich information which provided a deeper understanding and fresh insights into the landscape of the elderly and their healthcare needs. The interviews were recorded, transcribed and analysed thematically. The study found that elderly communities are not entirely averse to adoption of MMCs but challenges like affordability and chronic shortage of technical skills prove to be impediments to adoption of MMCs for the elderly’s healthcare. The lack of standardisation and data governance pertaining to data sharing in HISs also serve to exacerbate the matter. The study, therefore, recommends collaborative engagements amongst government, business and the elderly to facilitate the availability of affordable and accessible ICT infrastructure for the elderly communities. Improved adoption of MMCs carry the potential benefit which emanates from the assumption of a pro-active role by the elderly and optimising available MMCs thus reducing strain and freeing-up healthcare workers to concentrate on core duties. The onus thus falls on the healthcare sector to revise the available strategies which seek to enhance the quality of life of the elderly people living in the home environment. , Thesis (MCom) (Information Systems) -- University of Fort Hare, 2021.
- Full Text:
- Date Issued: 2021-09
- Authors: Fotoyi, Yolande Odwa
- Date: 2021-09
- Subjects: Medical informatics , Wireless communication systems in medical care
- Language: English
- Type: Master's/ theses , text
- Identifier: http://hdl.handle.net/10353/20406 , vital:45662
- Description: The gradual increase of elderly people around the globe necessitates intensive dialogue amongst government, the healthcare sector and elderly communities as per MPAA 2002 resolutions. Literature identifies technology as the enabler to drive the facilitation of improved living conditions beginning with an affordable, accessible and integrated health information system (HIS). The attainment of a better quality of care to meet the elderly’s needs requires the re-engineering of current modalities. The diverse nature of South Africa is more suited to a people-based rather than a process-centric approach currently in existence. Access barriers, affordability, the digital divide, lack of government buy-in, and fragmented HIS are considered major impediments to adoption of mobile monitoring and care systems (MMCs) for the elderly’s healthcare. Given the complications brought about by the Covid-19 pandemic, the adoption of MMCs cannot be more pronounced. However, despite available literature regarding elderly issues in both developed and developing countries, the elderly plight has still not been considered a national priority. The main purpose of this research was to investigate why elderly people do not adopt MMCs to improve their quality of life, with MMC technologies as a general area of research. The main objective of the study was to develop critical success factors to improve the adoption of MMCs by the elderly living at home. This would potentially alleviate the burden on healthcare resources and also improve the elderly’s quality of life. Primary data collection took place from 21 February to 28 February 2020 in Buffalo City Metropolitan Municipality. Semi-structured interviews were conducted with 15 participants comprising one male and 14 females who represented the elderly Black, Coloured, Indian and White people. This qualitative research tool and purposive sampling method were chosen in order to fully capture the participants’ experiences in the home environment, which excluded those living in frail care or step-down facilities or state institutions. Despite the sample size being small and not being generalizable, it delivered rich information which provided a deeper understanding and fresh insights into the landscape of the elderly and their healthcare needs. The interviews were recorded, transcribed and analysed thematically. The study found that elderly communities are not entirely averse to adoption of MMCs but challenges like affordability and chronic shortage of technical skills prove to be impediments to adoption of MMCs for the elderly’s healthcare. The lack of standardisation and data governance pertaining to data sharing in HISs also serve to exacerbate the matter. The study, therefore, recommends collaborative engagements amongst government, business and the elderly to facilitate the availability of affordable and accessible ICT infrastructure for the elderly communities. Improved adoption of MMCs carry the potential benefit which emanates from the assumption of a pro-active role by the elderly and optimising available MMCs thus reducing strain and freeing-up healthcare workers to concentrate on core duties. The onus thus falls on the healthcare sector to revise the available strategies which seek to enhance the quality of life of the elderly people living in the home environment. , Thesis (MCom) (Information Systems) -- University of Fort Hare, 2021.
- Full Text:
- Date Issued: 2021-09
A case study of Jeffrey Dahmer
- Authors: Freeman, Chanté F
- Date: 2021-04
- Subjects: Serial murders -- Case studies , Personality development -- Psychological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51032 , vital:43202
- Description: Jeffrey Lionel Dahmer is an infamous serial killer and cannibal. He was described as a normal, but shy little boy, yet developed into an adult who brutally murdered, engaged in necrophiliac acts, dismembered and ate parts of his victims. This study took the form of an intrinsic case study with the aim to explore and describe the personality development of Jeffrey Dahmer. This exploration examined Dahmer’s known childhood experiences through the theoretical perspective of Otto Kernberg’s (1974, 1975, 1976) Object Relations Theory, in order to understand how Dahmer’s personality was formed. Data was collected from multiple resources, including books, authoritative biographies, academic articles, and internet sources available in the public domain. It was analysed using Miles, Huberman, and Saldaña’s (2014) three-step process namely, data condensation, data display, and conclusion drawing and verification. This study’s findings confirmed the formation of pathological behavioural patterns supported by primitive defence mechanisms, associated with Kernberg’s lower level pathologies. It reflected Dahmer’s pathological formation of psychic structures seen in a diffuse identity and impaired formation of the superego, that supported these patterns. Additionally, the research was valuable in enhancing the researcher’s insight into personality development according to Kernberg’s Object Relations Theory. This study may also be beneficial to practising clinicians and professionals as an example of the early identification of personality pathology. , Thesis (MA) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Freeman, Chanté F
- Date: 2021-04
- Subjects: Serial murders -- Case studies , Personality development -- Psychological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51032 , vital:43202
- Description: Jeffrey Lionel Dahmer is an infamous serial killer and cannibal. He was described as a normal, but shy little boy, yet developed into an adult who brutally murdered, engaged in necrophiliac acts, dismembered and ate parts of his victims. This study took the form of an intrinsic case study with the aim to explore and describe the personality development of Jeffrey Dahmer. This exploration examined Dahmer’s known childhood experiences through the theoretical perspective of Otto Kernberg’s (1974, 1975, 1976) Object Relations Theory, in order to understand how Dahmer’s personality was formed. Data was collected from multiple resources, including books, authoritative biographies, academic articles, and internet sources available in the public domain. It was analysed using Miles, Huberman, and Saldaña’s (2014) three-step process namely, data condensation, data display, and conclusion drawing and verification. This study’s findings confirmed the formation of pathological behavioural patterns supported by primitive defence mechanisms, associated with Kernberg’s lower level pathologies. It reflected Dahmer’s pathological formation of psychic structures seen in a diffuse identity and impaired formation of the superego, that supported these patterns. Additionally, the research was valuable in enhancing the researcher’s insight into personality development according to Kernberg’s Object Relations Theory. This study may also be beneficial to practising clinicians and professionals as an example of the early identification of personality pathology. , Thesis (MA) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
Evaluation of medicinal potential of Boophone disticha (L.f.) Herb. used by the indigenous people in the Raymond Mhlaba Municipality Eastern Cape
- Authors: Gwayi, Yamkela
- Date: 2021-09
- Subjects: Bulbs (Plants) , Bulbs (Plant anatomy)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20612 , vital:46375
- Description: Boophone disticha (L.f.) Herb. is a bulbous plant native to southern African areas of the continent and spreads to tropical Africa. The plant has been noted in literature for its high toxicity and has a long lineage of its use in traditional medicine for the treatment of various diseases. Due to high demand in the conventional trade market, the plant's conservation status has been shown to be declining. The plant has been reported to cause human poisoning, which can lead to death, but the plant is still administered by an indigenous community to treat various diseases. This study investigated the therapeutic potential of B. disticha used by the people of Raymond Mhlaba municipality to show and verify its use in traditional medicine and how it can be used as a possible drug ingredient. This study was also set out to investigate the anti-mycobacterial activity of the plant for the first time for the B. disticha growing in the Eastern Cape region of South Africa. The phytochemical analysis of B. disticha was carried out on four different plant parts (i.e., roots, leaves, bulb inner and outer scales) and the plant was extracted using methanol and distilled water. The qualitative phytochemical analysis displayed the presence of tannins, flavonoids, phenols and alkaloids in all plant parts for both extraction solvents. Anthraquinones were absent in all the plant extracts. The detected phytochemicals (tannins, flavonoids, phenols and alkaloids) were quantified. The roots had the highest phytochemical content for methanol extract for each phytochemical tested [439.67±1.53 mg/g (QE) flavonoids, 2414.67±1.53 mg/g (GAE) phenols and 527.33±2.08 mg/g (GAE) alkaloids] compared to all the plant parts. Methanol extracts from B. disticha revealed highest phenolic contents (2414.67±1.53 mg/g GAE) for the roots, 1395.33±2.52 mg/g (GAE) for the bulb inner scales, 1560±1 mg/g (GAE) for the leaves and 1550.7±18.9 mg/g (GAE) for the bulb outer scales). The total flavonoid content of the bulb outer scales aqueous extract was not detected. The presence of significant amounts of phytochemical compounds indicates that B. disticha has a higher medicinal value and can be extensively investigated to extract bioactive ingredients that are useful to the society, and that could be sold for higher production than using synthetic drugs with side effects. The antimicrobial activity of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well micro-plates against nine disease-causing bacterial strains (5 Gram-negative and 4 Gram-positive) and three fungal isolates. The results revealed that methanol and aqueous extract of B. disticha demonstrated very good activity, with Gram-positive strains being more sensitive than Gram-negative ones. Boophone disticha aqueous extracts displayed the best activity against Staphylococcus aureus with MIC and MBC values ranging from 0.39 to 0.78 mg/ml. The methanolic extract of B. disticha leaves and outer scales of bulb displayed good activity against Klebsiella pneumoniae at MIC 0.78 mg/ml. The methanolic extract of B. disticha bulb outer scales also displayed good inhibition against Proteus vulgaris at MIC 0.78 mg/ml, with the root extract exhibiting activity against Shigella flexineri (MIC value of 0.078 mg/ml). Concerning antifungal activity, B. disticha extracts showed very poor inhibition properties against the fungal isolates. Anti-mycobacterium potential of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well microtiter plates. The plant parts tested were the roots, bulb inner scales, leaves and the bulb outer scales. The highest activity against Mycobacterium tuberculosis was observed with the root methanol extract at MIC 0.78 mg/ml. The anti-inflammatory properties of B. disticha were investigated using the 5-lypoxygenase (5- LOX) assay. The overall anti-inflammatory activity results for the B. disticha extracts were poor; at low concentrations, the plant displayed negative results. The leaves methanol extracts did show little activity at 0.4 mg/ml. , Thesis (MSc) (Botany) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Gwayi, Yamkela
- Date: 2021-09
- Subjects: Bulbs (Plants) , Bulbs (Plant anatomy)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20612 , vital:46375
- Description: Boophone disticha (L.f.) Herb. is a bulbous plant native to southern African areas of the continent and spreads to tropical Africa. The plant has been noted in literature for its high toxicity and has a long lineage of its use in traditional medicine for the treatment of various diseases. Due to high demand in the conventional trade market, the plant's conservation status has been shown to be declining. The plant has been reported to cause human poisoning, which can lead to death, but the plant is still administered by an indigenous community to treat various diseases. This study investigated the therapeutic potential of B. disticha used by the people of Raymond Mhlaba municipality to show and verify its use in traditional medicine and how it can be used as a possible drug ingredient. This study was also set out to investigate the anti-mycobacterial activity of the plant for the first time for the B. disticha growing in the Eastern Cape region of South Africa. The phytochemical analysis of B. disticha was carried out on four different plant parts (i.e., roots, leaves, bulb inner and outer scales) and the plant was extracted using methanol and distilled water. The qualitative phytochemical analysis displayed the presence of tannins, flavonoids, phenols and alkaloids in all plant parts for both extraction solvents. Anthraquinones were absent in all the plant extracts. The detected phytochemicals (tannins, flavonoids, phenols and alkaloids) were quantified. The roots had the highest phytochemical content for methanol extract for each phytochemical tested [439.67±1.53 mg/g (QE) flavonoids, 2414.67±1.53 mg/g (GAE) phenols and 527.33±2.08 mg/g (GAE) alkaloids] compared to all the plant parts. Methanol extracts from B. disticha revealed highest phenolic contents (2414.67±1.53 mg/g GAE) for the roots, 1395.33±2.52 mg/g (GAE) for the bulb inner scales, 1560±1 mg/g (GAE) for the leaves and 1550.7±18.9 mg/g (GAE) for the bulb outer scales). The total flavonoid content of the bulb outer scales aqueous extract was not detected. The presence of significant amounts of phytochemical compounds indicates that B. disticha has a higher medicinal value and can be extensively investigated to extract bioactive ingredients that are useful to the society, and that could be sold for higher production than using synthetic drugs with side effects. The antimicrobial activity of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well micro-plates against nine disease-causing bacterial strains (5 Gram-negative and 4 Gram-positive) and three fungal isolates. The results revealed that methanol and aqueous extract of B. disticha demonstrated very good activity, with Gram-positive strains being more sensitive than Gram-negative ones. Boophone disticha aqueous extracts displayed the best activity against Staphylococcus aureus with MIC and MBC values ranging from 0.39 to 0.78 mg/ml. The methanolic extract of B. disticha leaves and outer scales of bulb displayed good activity against Klebsiella pneumoniae at MIC 0.78 mg/ml. The methanolic extract of B. disticha bulb outer scales also displayed good inhibition against Proteus vulgaris at MIC 0.78 mg/ml, with the root extract exhibiting activity against Shigella flexineri (MIC value of 0.078 mg/ml). Concerning antifungal activity, B. disticha extracts showed very poor inhibition properties against the fungal isolates. Anti-mycobacterium potential of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well microtiter plates. The plant parts tested were the roots, bulb inner scales, leaves and the bulb outer scales. The highest activity against Mycobacterium tuberculosis was observed with the root methanol extract at MIC 0.78 mg/ml. The anti-inflammatory properties of B. disticha were investigated using the 5-lypoxygenase (5- LOX) assay. The overall anti-inflammatory activity results for the B. disticha extracts were poor; at low concentrations, the plant displayed negative results. The leaves methanol extracts did show little activity at 0.4 mg/ml. , Thesis (MSc) (Botany) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Effect of grazing systems and age on enteric methane production and performance plus grazing system effect on ruminal fermentation parameters of Nguni and Bonsmara beef cows
- Authors: Kayima, Denis
- Date: 2021-09
- Subjects: Grazing , Pastures--Management , Ruminants--Feeding and feeds
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20583 , vital:46153
- Description: Beef breeds of cattle are predominantly raised through extensive grazing and produce the highest levels of enteric methane (CH4) through ruminal fermentation. The objective of this study was to evaluate the effect of breed, grazing, and age on enteric methane production and performance of beef cows as well as effect of breed and grazing system on ruminal fermentation parameters. Two separate studies were conducted; the first study evaluated the effect of grazing system, breed, and age on ruminal enteric methane production and performance of selected beef breeds. The second study evaluated the effect of the grazing system and breed on ruminal fermentation. In the first study, two breeds (Nguni and Bonsmara) raised under two grazing systems (communal and commercial) in a random complete block design were grouped into 3 age categories. Eighty-four cows aged between 24-120 months were randomly selected and grouped by age: group A (young adult cow, n = 7, 24-48 months old), group B (adult, n = 7, 60-80 months old), and group C (old adult, n = 7, 90-120 months) in either grazing system. Data were collected on methane production per day, body condition, and weight used to estimate dry matter intake (DMI), methane per DMI, and methane per body weight. In the second study, 40 cows of the same age group, parity, and physiological status were used. Ten cows per breed (Bonsmara and Nguni) in either grazing system (communal and commercial) were used in a random complete block design. Rumen fluid collection was done by the stomach tube method. Data were collected on ruminal ammonia-nitrogen (NH3-N), pH, Temperature, volatile fatty acids (VFAs). Older animals produced higher methane per head in the order of C > B > A; (P<0.0001). Performance parameters like Body condition score (BCS), body weight (BW), dry matter intake (DMI), and daily methane were higher (P<0.05) in Bonsmara. However, Methane per BW was higher in Nguni (P<0.05), and methane per DMI did not differ among breeds (P>0.05). Body condition, BW, and DMI were higher (P<0.05) in commercial grazing system. Contrary, daily methane production, methane production per DMI, and methane produced per BW were higher in communal grazing. In the second study, Ruminal NH3-N concentration ranged between 69.05 - 96.78 mg/L and was higher (P<0.05) in Nguni. It was also higher (P<0.05) in the commercial grazing system. Ruminal pH, temperature, total VFAs, and certain individual VFAs (iso-butyrate, valeric and iso-valeric) did not differ (P>0.05) among breeds and grazing systems. However, total VFAs were slightly lower in communal grazing (78.87 mmol/L) than commercial grazing (89.80 mmol/L). Acetate, propionate, butyrate proportions, and acetate to propionate ratio did not differ (P>0.05) among breeds but differed (P<0.05) between grazing systems. Acetate (P<0.05) and acetate to propionate ratio (P<0.05) were higher in the communal grazing system, while the reverse was true for propionate (P<0.05) and butyrate (P<0.05). Bonsmara produced more methane than Nguni under similar conditions, with Nguni uniquely producing more methane per BW, indicating host genetics influence. Communal animals and older animals produced more CH4 than commercial and younger cows regardless of the breed, indicating ruminal inefficiencies in very old cows. These results emphasize the idea that host genetics or breed, grazing system, and age influence methane output and performance. Hence, breeding strategies for hard-to-measure traits like methane production need to put all these factors into consideration. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Kayima, Denis
- Date: 2021-09
- Subjects: Grazing , Pastures--Management , Ruminants--Feeding and feeds
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20583 , vital:46153
- Description: Beef breeds of cattle are predominantly raised through extensive grazing and produce the highest levels of enteric methane (CH4) through ruminal fermentation. The objective of this study was to evaluate the effect of breed, grazing, and age on enteric methane production and performance of beef cows as well as effect of breed and grazing system on ruminal fermentation parameters. Two separate studies were conducted; the first study evaluated the effect of grazing system, breed, and age on ruminal enteric methane production and performance of selected beef breeds. The second study evaluated the effect of the grazing system and breed on ruminal fermentation. In the first study, two breeds (Nguni and Bonsmara) raised under two grazing systems (communal and commercial) in a random complete block design were grouped into 3 age categories. Eighty-four cows aged between 24-120 months were randomly selected and grouped by age: group A (young adult cow, n = 7, 24-48 months old), group B (adult, n = 7, 60-80 months old), and group C (old adult, n = 7, 90-120 months) in either grazing system. Data were collected on methane production per day, body condition, and weight used to estimate dry matter intake (DMI), methane per DMI, and methane per body weight. In the second study, 40 cows of the same age group, parity, and physiological status were used. Ten cows per breed (Bonsmara and Nguni) in either grazing system (communal and commercial) were used in a random complete block design. Rumen fluid collection was done by the stomach tube method. Data were collected on ruminal ammonia-nitrogen (NH3-N), pH, Temperature, volatile fatty acids (VFAs). Older animals produced higher methane per head in the order of C > B > A; (P<0.0001). Performance parameters like Body condition score (BCS), body weight (BW), dry matter intake (DMI), and daily methane were higher (P<0.05) in Bonsmara. However, Methane per BW was higher in Nguni (P<0.05), and methane per DMI did not differ among breeds (P>0.05). Body condition, BW, and DMI were higher (P<0.05) in commercial grazing system. Contrary, daily methane production, methane production per DMI, and methane produced per BW were higher in communal grazing. In the second study, Ruminal NH3-N concentration ranged between 69.05 - 96.78 mg/L and was higher (P<0.05) in Nguni. It was also higher (P<0.05) in the commercial grazing system. Ruminal pH, temperature, total VFAs, and certain individual VFAs (iso-butyrate, valeric and iso-valeric) did not differ (P>0.05) among breeds and grazing systems. However, total VFAs were slightly lower in communal grazing (78.87 mmol/L) than commercial grazing (89.80 mmol/L). Acetate, propionate, butyrate proportions, and acetate to propionate ratio did not differ (P>0.05) among breeds but differed (P<0.05) between grazing systems. Acetate (P<0.05) and acetate to propionate ratio (P<0.05) were higher in the communal grazing system, while the reverse was true for propionate (P<0.05) and butyrate (P<0.05). Bonsmara produced more methane than Nguni under similar conditions, with Nguni uniquely producing more methane per BW, indicating host genetics influence. Communal animals and older animals produced more CH4 than commercial and younger cows regardless of the breed, indicating ruminal inefficiencies in very old cows. These results emphasize the idea that host genetics or breed, grazing system, and age influence methane output and performance. Hence, breeding strategies for hard-to-measure traits like methane production need to put all these factors into consideration. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27231 , vital:66479
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27231 , vital:66479
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26141 , vital:64919
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26141 , vital:64919
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26108 , vital:64901
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26108 , vital:64901
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
Main thesis title 2021: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: Grahamstown (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/52791 , vital:44031
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: Grahamstown (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/52791 , vital:44031
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26030 , vital:64782
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26030 , vital:64782
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Date Issued: 2021-04
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26230 , vital:64989
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26230 , vital:64989
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
Main thesis title map: subtitle if needed. If no subtitle follow instructions in manual
- Last name, First name (remember to update the ORCID)
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25428 , vital:64246
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text:
- Authors: Last name, First name (remember to update the ORCID)
- Date: 2021-04
- Subjects: 1 inch = 200 yards 30.5595° S, 22.9375° E , Grahamstown (South Africa) Maps , Eastern Cape (South Africa) Maps , South Africa History 1836-1909 Maps
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25428 , vital:64246
- Description: Abstract text. , Thesis (MA) -- Faculty of Humanities, Journalsim and Media Studies, 2021
- Full Text: