A case study of Jeffrey Dahmer
- Authors: Freeman, Chanté F
- Date: 2021-04
- Subjects: Serial murders -- Case studies , Personality development -- Psychological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51032 , vital:43202
- Description: Jeffrey Lionel Dahmer is an infamous serial killer and cannibal. He was described as a normal, but shy little boy, yet developed into an adult who brutally murdered, engaged in necrophiliac acts, dismembered and ate parts of his victims. This study took the form of an intrinsic case study with the aim to explore and describe the personality development of Jeffrey Dahmer. This exploration examined Dahmer’s known childhood experiences through the theoretical perspective of Otto Kernberg’s (1974, 1975, 1976) Object Relations Theory, in order to understand how Dahmer’s personality was formed. Data was collected from multiple resources, including books, authoritative biographies, academic articles, and internet sources available in the public domain. It was analysed using Miles, Huberman, and Saldaña’s (2014) three-step process namely, data condensation, data display, and conclusion drawing and verification. This study’s findings confirmed the formation of pathological behavioural patterns supported by primitive defence mechanisms, associated with Kernberg’s lower level pathologies. It reflected Dahmer’s pathological formation of psychic structures seen in a diffuse identity and impaired formation of the superego, that supported these patterns. Additionally, the research was valuable in enhancing the researcher’s insight into personality development according to Kernberg’s Object Relations Theory. This study may also be beneficial to practising clinicians and professionals as an example of the early identification of personality pathology. , Thesis (MA) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Freeman, Chanté F
- Date: 2021-04
- Subjects: Serial murders -- Case studies , Personality development -- Psychological aspects
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/51032 , vital:43202
- Description: Jeffrey Lionel Dahmer is an infamous serial killer and cannibal. He was described as a normal, but shy little boy, yet developed into an adult who brutally murdered, engaged in necrophiliac acts, dismembered and ate parts of his victims. This study took the form of an intrinsic case study with the aim to explore and describe the personality development of Jeffrey Dahmer. This exploration examined Dahmer’s known childhood experiences through the theoretical perspective of Otto Kernberg’s (1974, 1975, 1976) Object Relations Theory, in order to understand how Dahmer’s personality was formed. Data was collected from multiple resources, including books, authoritative biographies, academic articles, and internet sources available in the public domain. It was analysed using Miles, Huberman, and Saldaña’s (2014) three-step process namely, data condensation, data display, and conclusion drawing and verification. This study’s findings confirmed the formation of pathological behavioural patterns supported by primitive defence mechanisms, associated with Kernberg’s lower level pathologies. It reflected Dahmer’s pathological formation of psychic structures seen in a diffuse identity and impaired formation of the superego, that supported these patterns. Additionally, the research was valuable in enhancing the researcher’s insight into personality development according to Kernberg’s Object Relations Theory. This study may also be beneficial to practising clinicians and professionals as an example of the early identification of personality pathology. , Thesis (MA) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
A critical analysis of the application of South African business rescue provisions on small to medium enterprises (SMEs)
- Peteni, Ushimin https://orcid.org/ 0000-0001-6478-9862
- Authors: Peteni, Ushimin https://orcid.org/ 0000-0001-6478-9862
- Date: 2021-09
- Subjects: Small business , Business enterprises , Business failures--Law and legislation
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20328 , vital:45655
- Description: Small to Medium sized Enterprises (SMEs) play an essential role and can be described as the crucial bread and butter of the South African economy. They have the potential to create employment and skills development opportunities pertinent for sustainable economic growth. The unfortunate part is that South African Small to Medium sized Enterprises have the lowest survival rates in the world, resulting in high rates of business failure and job losses. This is because the South African economy does not favour SMEs, which are often burdened with structural hurdles including access to funding, lack of access to markets, inadequate skills, uncompetitive regulatory frameworks and technological disruptions. A developing economy such as South Africa cannot grow if companies facing financial difficulty are constantly liquidated. This is because the liquidation system does not offer companies an opportunity to restructure and possibly function within the context of raised concerns. In order to facilitate sustainable SMEs growth, the legislature has come up with statutory mechanisms to support SMEs and save them from total liquidation. Most recently, the Companies Act 71 of 2008 Chapter 6 introduces a business rescue mechanism applicable to all business structures established under the Act. The purpose of business rescue is to provide companies in financial distress with opportunities to reorganise, strategize and come up with useful corporate reorganisation measures which are useful and efficient in saving the financially distressed company and possibly yielding a better return for the creditors than would have been the case if the company was liquidated. This new enactment is seen as both an improvement and necessary reform from its predecessor, judicial administration which has widely been condemned as a failure. However, despite this improvement, the application of the current business provisions to SMEs is questionable. Although Chapter 6 of the Act makes provision for SMEs, it also presents a number of stumbling blocks, including the fact that services of business rescue practitioners are out of reach for most SMEs in South Africa. The purpose of this study is to consider challenges faced by SMEs in the practical application of the current business rescue provisions. This study further emphasizes the importance of South African SMEs, whilst looking at their challenges and how the Companies Act 71 of 2008 provisions, regarding business rescue, assists SMEs. The study looks at the impediments of certain provisions of business rescue and proposes recommendations. , Thesis (MA) (Laws) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Peteni, Ushimin https://orcid.org/ 0000-0001-6478-9862
- Date: 2021-09
- Subjects: Small business , Business enterprises , Business failures--Law and legislation
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20328 , vital:45655
- Description: Small to Medium sized Enterprises (SMEs) play an essential role and can be described as the crucial bread and butter of the South African economy. They have the potential to create employment and skills development opportunities pertinent for sustainable economic growth. The unfortunate part is that South African Small to Medium sized Enterprises have the lowest survival rates in the world, resulting in high rates of business failure and job losses. This is because the South African economy does not favour SMEs, which are often burdened with structural hurdles including access to funding, lack of access to markets, inadequate skills, uncompetitive regulatory frameworks and technological disruptions. A developing economy such as South Africa cannot grow if companies facing financial difficulty are constantly liquidated. This is because the liquidation system does not offer companies an opportunity to restructure and possibly function within the context of raised concerns. In order to facilitate sustainable SMEs growth, the legislature has come up with statutory mechanisms to support SMEs and save them from total liquidation. Most recently, the Companies Act 71 of 2008 Chapter 6 introduces a business rescue mechanism applicable to all business structures established under the Act. The purpose of business rescue is to provide companies in financial distress with opportunities to reorganise, strategize and come up with useful corporate reorganisation measures which are useful and efficient in saving the financially distressed company and possibly yielding a better return for the creditors than would have been the case if the company was liquidated. This new enactment is seen as both an improvement and necessary reform from its predecessor, judicial administration which has widely been condemned as a failure. However, despite this improvement, the application of the current business provisions to SMEs is questionable. Although Chapter 6 of the Act makes provision for SMEs, it also presents a number of stumbling blocks, including the fact that services of business rescue practitioners are out of reach for most SMEs in South Africa. The purpose of this study is to consider challenges faced by SMEs in the practical application of the current business rescue provisions. This study further emphasizes the importance of South African SMEs, whilst looking at their challenges and how the Companies Act 71 of 2008 provisions, regarding business rescue, assists SMEs. The study looks at the impediments of certain provisions of business rescue and proposes recommendations. , Thesis (MA) (Laws) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
A framework to improve social media as a communication tool in technical vocational education and training colleges in South Africa: a case of twitter.
- Nyamanhare, Sangudzayi Innocent
- Authors: Nyamanhare, Sangudzayi Innocent
- Date: 2021-02
- Subjects: Social media , Education, Higher , Universities and colleges
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20495 , vital:46008
- Description: South Africa uses social media as a communication tool. The use of Twitter as a communication tool in Technical Vocational Education and Training Colleges (TVET) in South Africa is a crucial factor for efficient and effective communication between TVET College administration and stakeholders such as prospective students, students, prospective employers, prospective employees and the Twitter community which follow various Twitter accounts. Literature has found that TVET Colleges use Twitter in a monologic manner which promotes one-way information flow. Twitter is also used in a limited fashion and within silos within TVET Colleges that have incorporated this social media platform into their communication strategy. The main purpose of this research study was to develop a framework to improve social media as a communication tool in TVET Colleges in South Africa. The Social Media-Integration- Theory-Model was used as the theoretical framework to evaluate the use of Twitter in TVET Colleges in South Africa, thus enabling the development of critical success factors to improve the use of social media as a communication tool. The Interpretivist paradigm and qualitative research approach was chosen for this study. Netnography was used to collect the online data from Twitter that was analysed in the study. Data was collected from 36 TVET Colleges in South Africa that have a Twitter account over a one-year period (April 2019 – March 2020). After the data collection, content and thematic analysis were used to analyse the date. The study developed a framework from the analyses of tweets which incorporated the four constructs from the Social-Media-Integration-Theory-Model. The study also developed five critical success factors, namely, procuring and maintaining of ICT infrastructure; implementing a policy that guide the use of social networks for communication purposes in TVET Colleges; raising awareness on the use of Twitter as a tool for communication; intensive training to manage administration of Twitter accounts and appointing champions to promote Twitter as a communication tool in TVET Colleges. , Thesis (MCom) (Information Systems)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Nyamanhare, Sangudzayi Innocent
- Date: 2021-02
- Subjects: Social media , Education, Higher , Universities and colleges
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20495 , vital:46008
- Description: South Africa uses social media as a communication tool. The use of Twitter as a communication tool in Technical Vocational Education and Training Colleges (TVET) in South Africa is a crucial factor for efficient and effective communication between TVET College administration and stakeholders such as prospective students, students, prospective employers, prospective employees and the Twitter community which follow various Twitter accounts. Literature has found that TVET Colleges use Twitter in a monologic manner which promotes one-way information flow. Twitter is also used in a limited fashion and within silos within TVET Colleges that have incorporated this social media platform into their communication strategy. The main purpose of this research study was to develop a framework to improve social media as a communication tool in TVET Colleges in South Africa. The Social Media-Integration- Theory-Model was used as the theoretical framework to evaluate the use of Twitter in TVET Colleges in South Africa, thus enabling the development of critical success factors to improve the use of social media as a communication tool. The Interpretivist paradigm and qualitative research approach was chosen for this study. Netnography was used to collect the online data from Twitter that was analysed in the study. Data was collected from 36 TVET Colleges in South Africa that have a Twitter account over a one-year period (April 2019 – March 2020). After the data collection, content and thematic analysis were used to analyse the date. The study developed a framework from the analyses of tweets which incorporated the four constructs from the Social-Media-Integration-Theory-Model. The study also developed five critical success factors, namely, procuring and maintaining of ICT infrastructure; implementing a policy that guide the use of social networks for communication purposes in TVET Colleges; raising awareness on the use of Twitter as a tool for communication; intensive training to manage administration of Twitter accounts and appointing champions to promote Twitter as a communication tool in TVET Colleges. , Thesis (MCom) (Information Systems)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
A review of factors influencing poor performance in matric examinations in Libode District, Eastern Cape
- Authors: Tsama, Vuyani
- Date: 2020-02
- Subjects: High school students , School improvement programs , Education--South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20506 , vital:46029
- Description: The researcher reviewed the factors that influence poor performance in matriculation examinations in the Libode District, also referred to as the OR Tambo Coastal District. Okoye (1982) defines poor academic performance of the individual or candidate in the learning situation as one in which a candidate fails to attain a set standard of performance in a given evaluation exercise such as a test, an examination or a series of continuous assessment. The research used a mixed method approach in an explanatory and case study research design. A purposive sampling of sixty (60) participants, who were the principals in the schools selected, was used. The researcher distributed forty-five (45) questionnaires and fifteen (15) interviews were conducted. The data analysis was done using Microsoft Excel for the quantitative data and thematic analysis for the qualitative data. Furthermore, inferential statistics were done, validating some statements in the research, and approving or disproving statements in the findings of the research. The perceptions of the causes of poor performance showed that forty percent (40 percent) of respondents disagreed that good planning was being done by the Department of Basic Education. Thirty-eight (38 percent ) percent of the participants also disagreed that low motivation on the part of the children was a factor in the poor results, followed by thirty-five percent (35 percent ) who slightly agreed that matric learners are failing because of socio-economic factors. Furthermore, on the challenges affecting the Department of Education, eight-seven percent (87 percent ) of the respondents supported the impact of drugs on poor performance, sixty-nine percent (69 percent) cited the issue of shortages of Learner Teaching and Support Material (LTSM) and sixty-five percent (65 percent) of respondents attributed the erratic and unfair progression of learners at grade eight (8), nine (9) and ten (10) level, which ultimately affected the grade twelve (12) results. The interview findings showed that the average pass rate was fifty percent (50 percent) in most schools, and the administration and leadership issues of the schools had been poorly managed. This, coupled with a shortage of LTSM and societal issues, such as drugs, negatively affected the matriculates. The research findings pointed to the challenge of drugs as being the biggest societal contributor to poor performance in matriculation results in OR Tambo Coastal District. The inferential statistics showed that gender could not affect the principal’s role as administrators and leader. However, gender did impact on leadership style. Women’s leadership style, which was more participative and consultative, was found to produce lower results; while male principals, who used a more bureaucratic, authoritative style, tended to produce higher results. Among other issues, the task-oriented, emotional nature of the principal’s job showed effects on school performance overall and the education level of the grade twelves (12), but educators alone could not affect the matric results. Furthermore, alone, the department’s efforts to improve results were not found to be productive; rather a number of factors could be combined to produce results. Lastly, the researcher recommended that the OR Tambo Coastal District should train teachers through development programmes, adjust the leadership style to one that is situational and transformational, and improve dialogue with school principals through regular school visits. Again, underperforming schools should be provided with LTSM, and teachers should be recruited and deployed on time. , Thesis (MPA) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2020-02
- Authors: Tsama, Vuyani
- Date: 2020-02
- Subjects: High school students , School improvement programs , Education--South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20506 , vital:46029
- Description: The researcher reviewed the factors that influence poor performance in matriculation examinations in the Libode District, also referred to as the OR Tambo Coastal District. Okoye (1982) defines poor academic performance of the individual or candidate in the learning situation as one in which a candidate fails to attain a set standard of performance in a given evaluation exercise such as a test, an examination or a series of continuous assessment. The research used a mixed method approach in an explanatory and case study research design. A purposive sampling of sixty (60) participants, who were the principals in the schools selected, was used. The researcher distributed forty-five (45) questionnaires and fifteen (15) interviews were conducted. The data analysis was done using Microsoft Excel for the quantitative data and thematic analysis for the qualitative data. Furthermore, inferential statistics were done, validating some statements in the research, and approving or disproving statements in the findings of the research. The perceptions of the causes of poor performance showed that forty percent (40 percent) of respondents disagreed that good planning was being done by the Department of Basic Education. Thirty-eight (38 percent ) percent of the participants also disagreed that low motivation on the part of the children was a factor in the poor results, followed by thirty-five percent (35 percent ) who slightly agreed that matric learners are failing because of socio-economic factors. Furthermore, on the challenges affecting the Department of Education, eight-seven percent (87 percent ) of the respondents supported the impact of drugs on poor performance, sixty-nine percent (69 percent) cited the issue of shortages of Learner Teaching and Support Material (LTSM) and sixty-five percent (65 percent) of respondents attributed the erratic and unfair progression of learners at grade eight (8), nine (9) and ten (10) level, which ultimately affected the grade twelve (12) results. The interview findings showed that the average pass rate was fifty percent (50 percent) in most schools, and the administration and leadership issues of the schools had been poorly managed. This, coupled with a shortage of LTSM and societal issues, such as drugs, negatively affected the matriculates. The research findings pointed to the challenge of drugs as being the biggest societal contributor to poor performance in matriculation results in OR Tambo Coastal District. The inferential statistics showed that gender could not affect the principal’s role as administrators and leader. However, gender did impact on leadership style. Women’s leadership style, which was more participative and consultative, was found to produce lower results; while male principals, who used a more bureaucratic, authoritative style, tended to produce higher results. Among other issues, the task-oriented, emotional nature of the principal’s job showed effects on school performance overall and the education level of the grade twelves (12), but educators alone could not affect the matric results. Furthermore, alone, the department’s efforts to improve results were not found to be productive; rather a number of factors could be combined to produce results. Lastly, the researcher recommended that the OR Tambo Coastal District should train teachers through development programmes, adjust the leadership style to one that is situational and transformational, and improve dialogue with school principals through regular school visits. Again, underperforming schools should be provided with LTSM, and teachers should be recruited and deployed on time. , Thesis (MPA) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2020-02
A trust framework to improve adoption of mobile banking by university students in South Africa
- Authors: van Heerden, Robyn
- Date: 2021-01
- Subjects: Mobile communication systems in education , Banks and banking, mobile , Education, Higher--Effect of technological innovations on
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20517 , vital:46073
- Description: Mobile banking, hereinafter referred to as m-banking, is a platform that allows individuals to conduct financial transactions using a mobile device at any given time. In today’s fast paced society, technologies have evolved to provide services that are convenient and benefit both the customer and service provider; these include m-banking. M-banking further encourages financial inclusion and economic growth, which are both crucial in South Africa. The aim of this research was to develop a trust framework to improve adoption of mobile banking by university students. This is an agency issue in the financial services industry as university students are a big segment of the future income earners and adopters of technology. Getting them to adopt and become regular m-banking users would significantly impact the revenue of banking institutions. The E-Commerce Acceptance Model and the Integrative Model of Organisational Trust were the two theoretical frameworks used as underlying theories to this study. A quantitative survey tool was employed to gather data at the University of Fort Hare in the Eastern Cape. After an extensive literature review, a questionnaire was formulated and distributed to students at the University of Fort Hare, of whom 471 responded. Descriptive and inferential statistics were used to analyse the data collected. Cronbach’s Alpha was employed to demonstrate the reliability of constructs used in the study and Pearson’s Product Moment. Correlation Coefficient r tested the strength of the relationships between these constructs. The study found that there are several factors that are significant, such as perceived usefulness, perceived ease of use, perceived risk when investigating trust and its influences on m-banking adoption amongst university students. As a result, the study recommends that banks should consider the above-mentioned factors in order to improve trust and increase m-banking adoption among university students in South Africa. , Thesis (MCom) (Information Systems) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-01
- Authors: van Heerden, Robyn
- Date: 2021-01
- Subjects: Mobile communication systems in education , Banks and banking, mobile , Education, Higher--Effect of technological innovations on
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20517 , vital:46073
- Description: Mobile banking, hereinafter referred to as m-banking, is a platform that allows individuals to conduct financial transactions using a mobile device at any given time. In today’s fast paced society, technologies have evolved to provide services that are convenient and benefit both the customer and service provider; these include m-banking. M-banking further encourages financial inclusion and economic growth, which are both crucial in South Africa. The aim of this research was to develop a trust framework to improve adoption of mobile banking by university students. This is an agency issue in the financial services industry as university students are a big segment of the future income earners and adopters of technology. Getting them to adopt and become regular m-banking users would significantly impact the revenue of banking institutions. The E-Commerce Acceptance Model and the Integrative Model of Organisational Trust were the two theoretical frameworks used as underlying theories to this study. A quantitative survey tool was employed to gather data at the University of Fort Hare in the Eastern Cape. After an extensive literature review, a questionnaire was formulated and distributed to students at the University of Fort Hare, of whom 471 responded. Descriptive and inferential statistics were used to analyse the data collected. Cronbach’s Alpha was employed to demonstrate the reliability of constructs used in the study and Pearson’s Product Moment. Correlation Coefficient r tested the strength of the relationships between these constructs. The study found that there are several factors that are significant, such as perceived usefulness, perceived ease of use, perceived risk when investigating trust and its influences on m-banking adoption amongst university students. As a result, the study recommends that banks should consider the above-mentioned factors in order to improve trust and increase m-banking adoption among university students in South Africa. , Thesis (MCom) (Information Systems) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-01
Agro-morphological characterization and investigations into the response of yellow Quality Protein Maize (QPM) genotypes to low soil pH
- Authors: Bobotyana, Simthandile
- Date: 2021-04
- Subjects: Corn as food , Plants--Drought tolerance
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20528 , vital:46087
- Description: Maize (Zea mays L) is an essential food grain crop in South Africa and is mainly grown under rainfed conditions by small-scale farmers. Quality Protein Maize (QPM) contains a higher level of two essential amino acids, tryptophan, and lysine when equated to normal maize. Therefore, the QPM in communities is a vital cereal where maize is a staple crop. Among the significant abiotic stresses that adversely affect maize productivity, such as soil fertility. Low soil pH or soil acidity results in nutrient deficiencies, as well as the toxicity of minerals such as manganese (Mn), aluminium (Al), and iron (Fe). The objectives of the study were to: (1) Characterize yellow QPM inbred lines and testcross hybrids for agro-morphological variability under optimum conditions, (2) Determine the response of QPM inbred lines and testcross hybrids to Al toxicity, and (3) Estimate the combining ability of QPM genotypes for the tolerance to Al toxicity. The laboratory assessment for tolerance to Al toxicity revealed a negative relationship between relative root growth RRG and hematoxylin staining; among the inbred lines, the genotype 8Y, 17Y, and 7Y had the highest RRG and net root growth (NRG) values and exhibited the highest tolerance to Al tolerance with the least hematoxylin staining. Inbred lines 12Y, 16Y, 19Y, 2Y, 20Y, 24Y, 30Y, 27Y, 34Y, 4Y, and 11Y were moderately tolerant. On the other hand, inbred lines 13Y, 18Y, and QSY5 had the least RRG were susceptible. Forty-four testcross hybrids were generated by crossing 22 QPM inbred lines with two open-pollinated variety (OPV) testers. These hybrids were evaluated together with five checks for different agronomic traits. The hybrids were planted in the summer season of 2017/18 and 2018/19 under rain-fed conditions 7 x 7 alpha lattice design was used. The significant variations among the genotypes for grain yield and most other agronomic traits under optimum, acidic conditions and across environments on combined analysis of variance. The hybrid was 14Y x NC (3.22 t/ha), followed by QSY5 x NC (3.02 t/ha) showed the highest yielding. The mean plant height was 226.23 cm, with ranges 197.60 to 254.85cm. The number of kernels per ear was 35, with a range from 29.45 to 40.25. Conversely, the mean ear length was 19.30 cm with a range of 17.65 to 20.95 cm, and the mean ear diameter ranged from 13,50 to 16,05 cm. The highest yielding hybrid, according to ranking, was 14Y x NC (2,93t/ha), which was ranked number 3, followed by 3Y x NC (2,83 t/ha), which was ranked number 4. Twenty-two yellow QPM inbred lines were evaluated for agro-morphological characteristics. A randomized complete block design with three replicates was used under acidic and normal soil conditions at two locations. The mean performance of inbred lines for grain yield ranged from 1.6 t/ha to 3.1 t/ha under optimum conditions, whereas it ranged from 1.1.t/ha to 1.7 t/ha under an acidic environment. The highest grain yielding genotypes under the optimum environment were 14Y, 7Y, and QSY5, while, in the acidic environment, the highest yielders were 3Y, QSY5, and 17Y. The lowest grain yielder under the optimum environment was 16Y at 1.5 t/ha, whereas 6Y, 7Y, 8Y, and 29Y were the most moderate yielders under the acidic environment. Grain yield displayed positive and significant (p<0,05) correlation with ear height (0.59), plant height (0.55), leaf length (0.18), number of leaves (0.37), number of ears per plot (0,54), number of kernels per row, number of rows per ear and thousand kernel weight. Combined ANOVA revealed that the environment effect was significant for all traits except the number of rows per ear and the number of ears in both environments. A total of 71 genotypes (22 inbred lines, forty-four single crosses, and five checks) were evaluated using a 7 x 7 alpha lattice design with two replications in combining ability. Across environments, the analysis showed a mean grain yield record of hybrids that ranged from 1.78 t/ha to 3.42 t/ha. Combined analysis showed only about 4 percent of the 44 hybrids had better grain yield than hybrid checks, whereas about 11 percent of the 44 hybrids had higher grain yield than two of the five hybrid checks. The highest yielding hybrid was 14Y x NC followed by QSY5 x NC. The Mid-parent heterosis (MPH) for grain yield ranged from -32.70 for 11YxObat to 16.50 percent for 14YxNC among the top-yielding genotypes. The highest positive mid-parent heterosis (MPH) for grain yield was observed in hybrid 14YxNC followed by QSY9xNC, QSY5xOBAT, 3YxNC, 13YxNC and 4YxOBAT, while the remaining hybrids exhibited negative estimates. Inbred lines' proportional contributions to the total variances were much higher than the testers for all traits. Whereas the inbred lines' proportional contribution was lower than the interaction of line x tester except on the number of ears which displayed the highest estimates of variances due to SCA effects, significant variation was observed. The GCA effects on grain yield in both environments generally showed no significant difference among inbred lines except for inbred lines such as QSY5 and 34Y but were positive. The selected crosses showed significant SCA effects in a few agronomic yield-related traits in the combined analysis. Among the hybrids, the only crosses that showed significant and positive SCA effects for grain yield were 25YxNC under optimum and 34YxObat under acidic environment. The inbred lines were assessed for tolerance to Al toxicity under laboratory conditions. Overall, the study identified soil acidity tolerant QPM inbred lines with an excellent combining ability for high-yielding QPM test hybrids, grain yield, and related traits. , Thesis (MSc) (Agriculture, Crop Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Bobotyana, Simthandile
- Date: 2021-04
- Subjects: Corn as food , Plants--Drought tolerance
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20528 , vital:46087
- Description: Maize (Zea mays L) is an essential food grain crop in South Africa and is mainly grown under rainfed conditions by small-scale farmers. Quality Protein Maize (QPM) contains a higher level of two essential amino acids, tryptophan, and lysine when equated to normal maize. Therefore, the QPM in communities is a vital cereal where maize is a staple crop. Among the significant abiotic stresses that adversely affect maize productivity, such as soil fertility. Low soil pH or soil acidity results in nutrient deficiencies, as well as the toxicity of minerals such as manganese (Mn), aluminium (Al), and iron (Fe). The objectives of the study were to: (1) Characterize yellow QPM inbred lines and testcross hybrids for agro-morphological variability under optimum conditions, (2) Determine the response of QPM inbred lines and testcross hybrids to Al toxicity, and (3) Estimate the combining ability of QPM genotypes for the tolerance to Al toxicity. The laboratory assessment for tolerance to Al toxicity revealed a negative relationship between relative root growth RRG and hematoxylin staining; among the inbred lines, the genotype 8Y, 17Y, and 7Y had the highest RRG and net root growth (NRG) values and exhibited the highest tolerance to Al tolerance with the least hematoxylin staining. Inbred lines 12Y, 16Y, 19Y, 2Y, 20Y, 24Y, 30Y, 27Y, 34Y, 4Y, and 11Y were moderately tolerant. On the other hand, inbred lines 13Y, 18Y, and QSY5 had the least RRG were susceptible. Forty-four testcross hybrids were generated by crossing 22 QPM inbred lines with two open-pollinated variety (OPV) testers. These hybrids were evaluated together with five checks for different agronomic traits. The hybrids were planted in the summer season of 2017/18 and 2018/19 under rain-fed conditions 7 x 7 alpha lattice design was used. The significant variations among the genotypes for grain yield and most other agronomic traits under optimum, acidic conditions and across environments on combined analysis of variance. The hybrid was 14Y x NC (3.22 t/ha), followed by QSY5 x NC (3.02 t/ha) showed the highest yielding. The mean plant height was 226.23 cm, with ranges 197.60 to 254.85cm. The number of kernels per ear was 35, with a range from 29.45 to 40.25. Conversely, the mean ear length was 19.30 cm with a range of 17.65 to 20.95 cm, and the mean ear diameter ranged from 13,50 to 16,05 cm. The highest yielding hybrid, according to ranking, was 14Y x NC (2,93t/ha), which was ranked number 3, followed by 3Y x NC (2,83 t/ha), which was ranked number 4. Twenty-two yellow QPM inbred lines were evaluated for agro-morphological characteristics. A randomized complete block design with three replicates was used under acidic and normal soil conditions at two locations. The mean performance of inbred lines for grain yield ranged from 1.6 t/ha to 3.1 t/ha under optimum conditions, whereas it ranged from 1.1.t/ha to 1.7 t/ha under an acidic environment. The highest grain yielding genotypes under the optimum environment were 14Y, 7Y, and QSY5, while, in the acidic environment, the highest yielders were 3Y, QSY5, and 17Y. The lowest grain yielder under the optimum environment was 16Y at 1.5 t/ha, whereas 6Y, 7Y, 8Y, and 29Y were the most moderate yielders under the acidic environment. Grain yield displayed positive and significant (p<0,05) correlation with ear height (0.59), plant height (0.55), leaf length (0.18), number of leaves (0.37), number of ears per plot (0,54), number of kernels per row, number of rows per ear and thousand kernel weight. Combined ANOVA revealed that the environment effect was significant for all traits except the number of rows per ear and the number of ears in both environments. A total of 71 genotypes (22 inbred lines, forty-four single crosses, and five checks) were evaluated using a 7 x 7 alpha lattice design with two replications in combining ability. Across environments, the analysis showed a mean grain yield record of hybrids that ranged from 1.78 t/ha to 3.42 t/ha. Combined analysis showed only about 4 percent of the 44 hybrids had better grain yield than hybrid checks, whereas about 11 percent of the 44 hybrids had higher grain yield than two of the five hybrid checks. The highest yielding hybrid was 14Y x NC followed by QSY5 x NC. The Mid-parent heterosis (MPH) for grain yield ranged from -32.70 for 11YxObat to 16.50 percent for 14YxNC among the top-yielding genotypes. The highest positive mid-parent heterosis (MPH) for grain yield was observed in hybrid 14YxNC followed by QSY9xNC, QSY5xOBAT, 3YxNC, 13YxNC and 4YxOBAT, while the remaining hybrids exhibited negative estimates. Inbred lines' proportional contributions to the total variances were much higher than the testers for all traits. Whereas the inbred lines' proportional contribution was lower than the interaction of line x tester except on the number of ears which displayed the highest estimates of variances due to SCA effects, significant variation was observed. The GCA effects on grain yield in both environments generally showed no significant difference among inbred lines except for inbred lines such as QSY5 and 34Y but were positive. The selected crosses showed significant SCA effects in a few agronomic yield-related traits in the combined analysis. Among the hybrids, the only crosses that showed significant and positive SCA effects for grain yield were 25YxNC under optimum and 34YxObat under acidic environment. The inbred lines were assessed for tolerance to Al toxicity under laboratory conditions. Overall, the study identified soil acidity tolerant QPM inbred lines with an excellent combining ability for high-yielding QPM test hybrids, grain yield, and related traits. , Thesis (MSc) (Agriculture, Crop Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-04
An enquiry on gender, religion and sport: the case of Muslim women residing in Malabar, Port Elizabeth
- Authors: Sait, Shaabiera
- Date: 2020-12
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55196 , vital:49588
- Description: Modern sports have made many claims, one of which is to give surety of equality within sporting codes. They also specifically focus on providing equal rights to allow individuals to compete in sports. The political history of South Africa is particularly important as it is essentially linked to the capacity to participate in sport in South Africa. Therefore, it is safe to say that politics and sports are intrinsically entwined in the country. Sport played a vibrant role in the struggle against the system of apartheid and has a vigorous role to play in the transformation and nation-building of South Africa. Islam has been open to growth and development, adaptation, and even change. Since its revelation, Islam has been filtered through a succession of cultural growth, reflecting the complex ways in which religious beliefs and social reality accommodate one another. Islam possesses a unifying framework which is provided by the Quran (Islam’s primary source regarded by Muslims as God’s revealed word) as the quintessential source along with the Hadith (the statements and deeds of Prophet Muhammad [Peace be unto him]) and language of the religion of Islam. This unity is accompanied by a multitude of diversities that must be considered in any discussion regarding Islam and its practice by Muslims. The Muslim women presented in this thesis were specifically from the Malabar area in Port Elizabeth, where different interpretations or perspectives of Islam exist. Malabar was created in 1961 under the Group Areas Act of 1950 in terms of which the Apartheid government forced people of different race categories to live separately. In a variety of societies and communities where legislation, customs, and traditions are affected or inspired by interpretations of the Quran and Sunnah, they combine to define the roles and status of men and women. Within a specific Muslim society, however, these may vary from one class or generation to another. Muslim women’s lives and the choices they face are influenced as much by patriarchal social arrangements as they are by religious ideology. The research is centred around the interpretations of Islam and what these interpretations mean for Muslim women and their participation in sports, while also considering the interconnectedness of personal choice and the rules and regulations of Islam. The objective of this study was to critically analyse the effect that the different interpretations of Islam have on Muslim women’s participation in sporting activities, as iii well as the barriers that Muslim women face during their involvement in sports. The research adopted a qualitative research method in which 20 Muslim women were interviewed, with the use of semi-structured interview schedules, as well as participant observation to collect the necessary data. A case study method was used to share the data collected from interviews. Furthermore, an auto-ethnographic method is also utilized for self-reflection and to explore personal experience relevant to this research. This thesis also draws on the Marxist feminist theoretical approach, focusing on investigating and explaining how women are oppressed through systems of capitalism and private property. Marxism and feminism are theories of power, which is distributed unequally in society. In researching gender, religion, and sport, I found that the relationship between Muslim women and sport is understood in different ways because of different interpretations of Islam and its teachings, often between one generation to the next and amongst various Muslim communities. Thus, individuals who are passionate about their sport will go the extra mile to ensure participation. In some cases, this would be regardless of the rules and regulations of Islam. Muslim women across the world have made many strides and overcome many barriers in sport. Over the years, although not favourably portrayed in the media, participation by women has increased drastically over time. This thesis explores gender, religion, and sport as well as the ‘lived body’ experiences of veiled Muslim women in sport and physical activity. I attempt to explain the relationship between religious requirements and participation in sport and physical activity. , Thesis (MA) -- Faculty of Humanities, School of Governmental and Social Sciences, 2020
- Full Text:
- Date Issued: 2020-12
- Authors: Sait, Shaabiera
- Date: 2020-12
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55196 , vital:49588
- Description: Modern sports have made many claims, one of which is to give surety of equality within sporting codes. They also specifically focus on providing equal rights to allow individuals to compete in sports. The political history of South Africa is particularly important as it is essentially linked to the capacity to participate in sport in South Africa. Therefore, it is safe to say that politics and sports are intrinsically entwined in the country. Sport played a vibrant role in the struggle against the system of apartheid and has a vigorous role to play in the transformation and nation-building of South Africa. Islam has been open to growth and development, adaptation, and even change. Since its revelation, Islam has been filtered through a succession of cultural growth, reflecting the complex ways in which religious beliefs and social reality accommodate one another. Islam possesses a unifying framework which is provided by the Quran (Islam’s primary source regarded by Muslims as God’s revealed word) as the quintessential source along with the Hadith (the statements and deeds of Prophet Muhammad [Peace be unto him]) and language of the religion of Islam. This unity is accompanied by a multitude of diversities that must be considered in any discussion regarding Islam and its practice by Muslims. The Muslim women presented in this thesis were specifically from the Malabar area in Port Elizabeth, where different interpretations or perspectives of Islam exist. Malabar was created in 1961 under the Group Areas Act of 1950 in terms of which the Apartheid government forced people of different race categories to live separately. In a variety of societies and communities where legislation, customs, and traditions are affected or inspired by interpretations of the Quran and Sunnah, they combine to define the roles and status of men and women. Within a specific Muslim society, however, these may vary from one class or generation to another. Muslim women’s lives and the choices they face are influenced as much by patriarchal social arrangements as they are by religious ideology. The research is centred around the interpretations of Islam and what these interpretations mean for Muslim women and their participation in sports, while also considering the interconnectedness of personal choice and the rules and regulations of Islam. The objective of this study was to critically analyse the effect that the different interpretations of Islam have on Muslim women’s participation in sporting activities, as iii well as the barriers that Muslim women face during their involvement in sports. The research adopted a qualitative research method in which 20 Muslim women were interviewed, with the use of semi-structured interview schedules, as well as participant observation to collect the necessary data. A case study method was used to share the data collected from interviews. Furthermore, an auto-ethnographic method is also utilized for self-reflection and to explore personal experience relevant to this research. This thesis also draws on the Marxist feminist theoretical approach, focusing on investigating and explaining how women are oppressed through systems of capitalism and private property. Marxism and feminism are theories of power, which is distributed unequally in society. In researching gender, religion, and sport, I found that the relationship between Muslim women and sport is understood in different ways because of different interpretations of Islam and its teachings, often between one generation to the next and amongst various Muslim communities. Thus, individuals who are passionate about their sport will go the extra mile to ensure participation. In some cases, this would be regardless of the rules and regulations of Islam. Muslim women across the world have made many strides and overcome many barriers in sport. Over the years, although not favourably portrayed in the media, participation by women has increased drastically over time. This thesis explores gender, religion, and sport as well as the ‘lived body’ experiences of veiled Muslim women in sport and physical activity. I attempt to explain the relationship between religious requirements and participation in sport and physical activity. , Thesis (MA) -- Faculty of Humanities, School of Governmental and Social Sciences, 2020
- Full Text:
- Date Issued: 2020-12
An evaluation of performance management development system alignment with organizational objectives: case study of the Department of Tourism.
- Authors: Mkhavele, Enes Katekani
- Date: 2021-07
- Subjects: Performance--Management , Organizational effectiveness , Employees--Rating of
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21455 , vital:48645
- Description: The aim and objectives of this study are to assess the public sectors’ performance management development framework’s role in fulfilling organizational and employees’ performance objectives within Department of Tourism, to identify areas of disconnect between the Department of Tourism’s strategic objectives and employee performance, to determine whether the current employee rewards system enhances organizational commitment and promotes organizational effectiveness. Lastly, the study seeks to make a recommendation on how the Department of Tourism can align its employee’s performance management systems with the organizational performance planning process. , Thesis (PhD) (Public Administration) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-07
- Authors: Mkhavele, Enes Katekani
- Date: 2021-07
- Subjects: Performance--Management , Organizational effectiveness , Employees--Rating of
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21455 , vital:48645
- Description: The aim and objectives of this study are to assess the public sectors’ performance management development framework’s role in fulfilling organizational and employees’ performance objectives within Department of Tourism, to identify areas of disconnect between the Department of Tourism’s strategic objectives and employee performance, to determine whether the current employee rewards system enhances organizational commitment and promotes organizational effectiveness. Lastly, the study seeks to make a recommendation on how the Department of Tourism can align its employee’s performance management systems with the organizational performance planning process. , Thesis (PhD) (Public Administration) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-07
An Updated Catalogue and Review of Afrotherian (Afroplacentalia) Mammals in the Amathole Museum Collection, Eastern Cape Province, South Africa
- Authors: Mahala, Buyiswa
- Date: 2021-03
- Subjects: Mammals
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20741 , vital:46513
- Description: The Mammalogy Collection of the Amathole Museum is one of the most comprehensive mammal collections in Africa, with a specialisation in southern African fauna. It was accumulated over almost 100 years, in some instances from areas that no longer support sustainable populations of wildlife. The collection, therefore, is irreplaceable. A museum collection is only as good as its accessibility to students and researchers for it to perform its functions as a biodiversity archive. Accessibility, in turn, depends on up-to-date taxonomy and classification; good curation practices; public awareness of the collection; and electronic access to the details of the collection’s composition. The higher-level taxonomy of the Class Mammalia has undergone extensive changes since the adoption of molecular systematic techniques. Phylogenetic reconstructions based on large DNA sequence databases consistently group placental mammals into four superorders: Laurasiatheria (carnivores, bats, ungulates, whales); Euarchontoglires, a super-clade that includes primates (apes, monkeys and allies), rodents (mouse, rat and guinea pig), lagomorphs, (rabbit and hares), dermopterans (flying lemurs or colugos) and scandentians (tree shrew); Xenarthra (armadillos, anteaters, sloths), and Afrotheria (elephants, sea cows, tenrecs, aardvarks, sengis, golden moles, hyraxes). New relationships have been recognised that have required the erection of new taxonomic groups. Most of the Amathole Museum mammal collection was assembled and accessioned long before this new system was devised, and I undertook this project to begin the re-organisation of the collection according to the new information. I conducted and audit and inventory of all specimens of Afrotheria, or the mammals of African origin, housed in the Amathole Museum collection. I photographed the specimens and corrected and updated their taxonomic details. As we now live in an age of digital information, natural history collections need to be available online to extend public access. Specimen details were entered into Excel files, and their localities were mapped. This kind of information has the possibility of transforming biological curricula to include more authentic and inquiry-driven educational experiences. My future goals are to acquire Malagasy tenrecs for the Amathole Afrotheria collection, and to investigate the taxonomic validity of the Amathole hyrax collection, as many specimens were found with confusing and out-of-date classifications. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-03
- Authors: Mahala, Buyiswa
- Date: 2021-03
- Subjects: Mammals
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20741 , vital:46513
- Description: The Mammalogy Collection of the Amathole Museum is one of the most comprehensive mammal collections in Africa, with a specialisation in southern African fauna. It was accumulated over almost 100 years, in some instances from areas that no longer support sustainable populations of wildlife. The collection, therefore, is irreplaceable. A museum collection is only as good as its accessibility to students and researchers for it to perform its functions as a biodiversity archive. Accessibility, in turn, depends on up-to-date taxonomy and classification; good curation practices; public awareness of the collection; and electronic access to the details of the collection’s composition. The higher-level taxonomy of the Class Mammalia has undergone extensive changes since the adoption of molecular systematic techniques. Phylogenetic reconstructions based on large DNA sequence databases consistently group placental mammals into four superorders: Laurasiatheria (carnivores, bats, ungulates, whales); Euarchontoglires, a super-clade that includes primates (apes, monkeys and allies), rodents (mouse, rat and guinea pig), lagomorphs, (rabbit and hares), dermopterans (flying lemurs or colugos) and scandentians (tree shrew); Xenarthra (armadillos, anteaters, sloths), and Afrotheria (elephants, sea cows, tenrecs, aardvarks, sengis, golden moles, hyraxes). New relationships have been recognised that have required the erection of new taxonomic groups. Most of the Amathole Museum mammal collection was assembled and accessioned long before this new system was devised, and I undertook this project to begin the re-organisation of the collection according to the new information. I conducted and audit and inventory of all specimens of Afrotheria, or the mammals of African origin, housed in the Amathole Museum collection. I photographed the specimens and corrected and updated their taxonomic details. As we now live in an age of digital information, natural history collections need to be available online to extend public access. Specimen details were entered into Excel files, and their localities were mapped. This kind of information has the possibility of transforming biological curricula to include more authentic and inquiry-driven educational experiences. My future goals are to acquire Malagasy tenrecs for the Amathole Afrotheria collection, and to investigate the taxonomic validity of the Amathole hyrax collection, as many specimens were found with confusing and out-of-date classifications. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-03
Assessing the effects of the South African schools rationalisation policy on educator morale-case study of Buffalo City District Education in King Williams Town – Eastern Cape
- Authors: Mgedezi, Nomahlubi Pamela
- Date: 2021-09
- Subjects: Teacher morale
- Language: English
- Type: Master's/ theses , text
- Identifier: http://hdl.handle.net/10353/20439 , vital:45666
- Description: The purpose of the study was to scrutinise and assess the effects of South African school’s rationalization framework on educator morale in the Eastern Cape Department of Basic Education amidst the research objectives. The researcher wanted to establish if the Department of Education had succeeded in the implementation of the policy. In terms of the accomplishment of the objectives the researcher wanted to determine whether the exercise of the policy had affected the morale of educators. The study reviewed the literature, theoretical and empirical assessment of rationalisation process on the morale of educators in the King William’s Town district in the Eastern Cape. The study adopted a case study design of qualitative approach. The target population of the study included seventeen educators of the five schools in King William’s Town. A purposive sampling was used to select the respondents. A semi – structured interviews and questionnaires was used, and data was analysed using thematic approach according to the themes from the research questions. The questionnaire method succeeded by interviews was used for the collection of data. The tables and bar graphs were used to represent the number of respondents that participated in the study. The findings denoted that some of the educators believed the Department of Education had not planned the process of rationalisation sufficiently as it brought about anxiety and stress, though some of the affected educators mentioned that it brought relief as there are additional educators to teach other learning areas. The study recommended that the Department of basic Education in Eastern Cape should as a matter of priority provide maximum support to the affected educators. The study concluded that it became evident that the interaction with the respondents showed that the rationalisation process affects redeployed educators negatively. The outcome of the study could be helpful to the Department of Education to come up with strategies of implementing the policy and serve as a centre of reference towards other organisations and unions. , Thesis (MPA) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Mgedezi, Nomahlubi Pamela
- Date: 2021-09
- Subjects: Teacher morale
- Language: English
- Type: Master's/ theses , text
- Identifier: http://hdl.handle.net/10353/20439 , vital:45666
- Description: The purpose of the study was to scrutinise and assess the effects of South African school’s rationalization framework on educator morale in the Eastern Cape Department of Basic Education amidst the research objectives. The researcher wanted to establish if the Department of Education had succeeded in the implementation of the policy. In terms of the accomplishment of the objectives the researcher wanted to determine whether the exercise of the policy had affected the morale of educators. The study reviewed the literature, theoretical and empirical assessment of rationalisation process on the morale of educators in the King William’s Town district in the Eastern Cape. The study adopted a case study design of qualitative approach. The target population of the study included seventeen educators of the five schools in King William’s Town. A purposive sampling was used to select the respondents. A semi – structured interviews and questionnaires was used, and data was analysed using thematic approach according to the themes from the research questions. The questionnaire method succeeded by interviews was used for the collection of data. The tables and bar graphs were used to represent the number of respondents that participated in the study. The findings denoted that some of the educators believed the Department of Education had not planned the process of rationalisation sufficiently as it brought about anxiety and stress, though some of the affected educators mentioned that it brought relief as there are additional educators to teach other learning areas. The study recommended that the Department of basic Education in Eastern Cape should as a matter of priority provide maximum support to the affected educators. The study concluded that it became evident that the interaction with the respondents showed that the rationalisation process affects redeployed educators negatively. The outcome of the study could be helpful to the Department of Education to come up with strategies of implementing the policy and serve as a centre of reference towards other organisations and unions. , Thesis (MPA) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Bio-utilization of keratinous waste biomass for the production of keratinolytic proteases by Chryseobactreium aquifrigidense isolated from poultry waste dumpsite
- Authors: Bokveld, Amahle
- Date: 2021-02
- Subjects: Keratin
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20550 , vital:46120
- Description: Keratin is an insoluble and a fibrous protein that is mostly found in feathers, animal wool, and hair, making them mechanically stable. Avian feathers are the most ubiquitously generated keratinous waste biomass from the poultry processing plants. Keratinous waste biomass valorization could produce amino acids and bioactive peptides. Hence, the bio-recycling of keratin-rich wastes bears an advantage over the chemical and thermal means. In recent times, the microbial keratinases continues to gain traction due to the litany of their potential significance in green technology. Consequently, this study assessed chicken feather degrading and keratinase production potentials of bacteria isolated from a local poultry dumpsite. Soil samples were collected from the poultry dumpsite. Bacteria were isolated using basal salt medium and screened for keratinolytic activity. The identification of potent chicken feather degrading bacterial isolates was through 16S rRNA gene sequence analysis. Keratinase production efficiency of isolates on chicken feather constituted medium was optimized. Hydrolysate's amino acid compositions were quantified, and the keratinases produced was characterized. Out of 22 bacteria isolated from the soil samples, 12 showed a varying degree of proteolytic activity on skimmed milk agar. Four (4) isolates, coded as FPS-01, FPS-07, FPS-09, WDS-06, out of the 12 proteolytic strains further displayed remarkable degradation of the intact chicken feather; percentage degradation ranged from 69 percent for FPS-01 to 88 percent for FPS-09. The extracellular keratinase activity ranged from 610.91 ± 56.57 U/mL for WDS-06 and 834.55 ± 66.86 U/mL for FPS-07. Additionally, the sulfhydryl group concentration quantified from the fermentation broth was 2.22 ± 0.37 (mM), 0.22 ± 0.08 (mM), 2.27 ± 0.09 (mM), and 2.76 ± 0.79 (mM) for FPS-01, FPS-07, FPS-09 and WDS-06, respectively. Based on 16S rRNA gene sequencing and phylogenetic analyses, the isolates FPS-07 and FPS-09 were identified as Chryseobacterium aquifrigidense FANN1 and Chryseobacterium aquifrigidense FANN2. Following the optimization process, the improved fermentation conditions were pH 6, inoculum side (4 percent, v/v), temperature (30 oC), and chicken feather (0.5-1.5 percent, w/v) for FANN1. FANN2 optimal fermentation conditions were pH 6, inoculum (5 percent, v/v), temperature (30 oC), and chicken feather (1.5 percent, w/v). Both bacterial isolates showed the highest extracellular keratinase production after 72 h of the fermentation time. Analysis of the hydrolysates generated from the bacteria fermentation showed a high concentration of arginine, serine, glutamic acid, glycine, proline, valine, and leucine at a respective concentration of 1.13, 1.02, 0.83, 0.94, 0.85, 0.84, and 0.86 (g/100g sample) against FANN1. Similarly, FANN2 generated hydrolysate showed high concentrations of glutamic acid, arginine, serine, aspartic acid, and glycine at this respective abundance 2.52, 1.92, 2.12, 2.25 and 1.9 (g/100g sample). Keratinases from FANN1 and FANN2 showed optimal catalytic efficiency at pH 8 and temperature between 40-50 oC. The enzyme was considerably thermostable at 40 oC and 50 oC after 120 min of preheating. Both FANN1 and FANN2 showed variable residual activity in the presence of the different metal ions. Keratinase from FANN1 recorded the following residual activity of Fe3+ (120 ± 5.06 percent), Ca2+ (100 ± 10.33 percent), Na+ (122 ± 2.95 percent), Al3+ (106 ± 10.33 percent). Likewise, FANN2 keratinase showed remarkable stability against Na+ (108 ± 13.71 percent), Ba2+ (102 ± 0.86 percent), Al3+ (105 ± 2.57 percent), and Ca2+ (96 ± 2.99 percent). Keratinase from FANN1 was catalytically activated after 60 min of pre-treatment with the following detergents, Sunlight (129 percent), Ariel (116 percent), MAQ (151 percent), and Surf (143 percent) compared to the control. FANN2 keratinase showed less stability with laundry detergents after 60 min of preincubation. FANN1 keratinase showed remarkable stability in the presence of chemical agents tested, with residual activity of 90 ± 0.18 percent, 105 ± 7.55 percent, 108 ± 4.31 percent, 123 ± 1.44 percent, 132 ± 1.26 percent, 96 ± 7.19 percent, and 101 ± 3.06 percent for DTT, hydrogen peroxide, DMSO, acetonitrile, triton X-100, tween-80, and SDS, respectively. The enzyme activity was also considerably inhibited by PMSF and EDTA, which suggested a mixed type of protease. Furthermore, keratinase from FANN2 was inhibited by EDTA, and such inhibition pattern grouped it as a metallo-type of protease. The enzyme was also stable in the presence of other chemical agents tested. Therefore, the findings suggest the isolates and their enzymes' relevance to sustainable recycling of recalcitrant keratinous wastes into high-value products with immense application potentials. The remarkable stability shown by keratinases from FANN1 and FANN2, post detergent and chemical agents pre-treatment, indicates promise for the biotechnology and industrial sector. , Thesis(MSc) (Microbiology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Bokveld, Amahle
- Date: 2021-02
- Subjects: Keratin
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20550 , vital:46120
- Description: Keratin is an insoluble and a fibrous protein that is mostly found in feathers, animal wool, and hair, making them mechanically stable. Avian feathers are the most ubiquitously generated keratinous waste biomass from the poultry processing plants. Keratinous waste biomass valorization could produce amino acids and bioactive peptides. Hence, the bio-recycling of keratin-rich wastes bears an advantage over the chemical and thermal means. In recent times, the microbial keratinases continues to gain traction due to the litany of their potential significance in green technology. Consequently, this study assessed chicken feather degrading and keratinase production potentials of bacteria isolated from a local poultry dumpsite. Soil samples were collected from the poultry dumpsite. Bacteria were isolated using basal salt medium and screened for keratinolytic activity. The identification of potent chicken feather degrading bacterial isolates was through 16S rRNA gene sequence analysis. Keratinase production efficiency of isolates on chicken feather constituted medium was optimized. Hydrolysate's amino acid compositions were quantified, and the keratinases produced was characterized. Out of 22 bacteria isolated from the soil samples, 12 showed a varying degree of proteolytic activity on skimmed milk agar. Four (4) isolates, coded as FPS-01, FPS-07, FPS-09, WDS-06, out of the 12 proteolytic strains further displayed remarkable degradation of the intact chicken feather; percentage degradation ranged from 69 percent for FPS-01 to 88 percent for FPS-09. The extracellular keratinase activity ranged from 610.91 ± 56.57 U/mL for WDS-06 and 834.55 ± 66.86 U/mL for FPS-07. Additionally, the sulfhydryl group concentration quantified from the fermentation broth was 2.22 ± 0.37 (mM), 0.22 ± 0.08 (mM), 2.27 ± 0.09 (mM), and 2.76 ± 0.79 (mM) for FPS-01, FPS-07, FPS-09 and WDS-06, respectively. Based on 16S rRNA gene sequencing and phylogenetic analyses, the isolates FPS-07 and FPS-09 were identified as Chryseobacterium aquifrigidense FANN1 and Chryseobacterium aquifrigidense FANN2. Following the optimization process, the improved fermentation conditions were pH 6, inoculum side (4 percent, v/v), temperature (30 oC), and chicken feather (0.5-1.5 percent, w/v) for FANN1. FANN2 optimal fermentation conditions were pH 6, inoculum (5 percent, v/v), temperature (30 oC), and chicken feather (1.5 percent, w/v). Both bacterial isolates showed the highest extracellular keratinase production after 72 h of the fermentation time. Analysis of the hydrolysates generated from the bacteria fermentation showed a high concentration of arginine, serine, glutamic acid, glycine, proline, valine, and leucine at a respective concentration of 1.13, 1.02, 0.83, 0.94, 0.85, 0.84, and 0.86 (g/100g sample) against FANN1. Similarly, FANN2 generated hydrolysate showed high concentrations of glutamic acid, arginine, serine, aspartic acid, and glycine at this respective abundance 2.52, 1.92, 2.12, 2.25 and 1.9 (g/100g sample). Keratinases from FANN1 and FANN2 showed optimal catalytic efficiency at pH 8 and temperature between 40-50 oC. The enzyme was considerably thermostable at 40 oC and 50 oC after 120 min of preheating. Both FANN1 and FANN2 showed variable residual activity in the presence of the different metal ions. Keratinase from FANN1 recorded the following residual activity of Fe3+ (120 ± 5.06 percent), Ca2+ (100 ± 10.33 percent), Na+ (122 ± 2.95 percent), Al3+ (106 ± 10.33 percent). Likewise, FANN2 keratinase showed remarkable stability against Na+ (108 ± 13.71 percent), Ba2+ (102 ± 0.86 percent), Al3+ (105 ± 2.57 percent), and Ca2+ (96 ± 2.99 percent). Keratinase from FANN1 was catalytically activated after 60 min of pre-treatment with the following detergents, Sunlight (129 percent), Ariel (116 percent), MAQ (151 percent), and Surf (143 percent) compared to the control. FANN2 keratinase showed less stability with laundry detergents after 60 min of preincubation. FANN1 keratinase showed remarkable stability in the presence of chemical agents tested, with residual activity of 90 ± 0.18 percent, 105 ± 7.55 percent, 108 ± 4.31 percent, 123 ± 1.44 percent, 132 ± 1.26 percent, 96 ± 7.19 percent, and 101 ± 3.06 percent for DTT, hydrogen peroxide, DMSO, acetonitrile, triton X-100, tween-80, and SDS, respectively. The enzyme activity was also considerably inhibited by PMSF and EDTA, which suggested a mixed type of protease. Furthermore, keratinase from FANN2 was inhibited by EDTA, and such inhibition pattern grouped it as a metallo-type of protease. The enzyme was also stable in the presence of other chemical agents tested. Therefore, the findings suggest the isolates and their enzymes' relevance to sustainable recycling of recalcitrant keratinous wastes into high-value products with immense application potentials. The remarkable stability shown by keratinases from FANN1 and FANN2, post detergent and chemical agents pre-treatment, indicates promise for the biotechnology and industrial sector. , Thesis(MSc) (Microbiology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
Capital flows and real exchange rate movement in South Africa
- Authors: Lindani, Sandiswa
- Date: 2021-09
- Subjects: Foreign exchange rates , Exchange rate pass-through , Capital movements
- Language: English
- Type: Master's/ theses , text
- Identifier: http://hdl.handle.net/10353/20417 , vital:45664
- Description: The neoclassical theory suggests that free flow of external capital should be equilibrating and thereby facilitating smoothening of an economy's consumption or production patterns. Based on this background, this study empirically examines the extent to which the different forms of capital flows amongst other variables influence the real exchange rate in South Africa for the period 1980 to 2019, using annual data. Estimation techniques employed include the Johansen Co-integration Approach and the Vector error correction model (VECM). The variables were found to be co-integrated, with six co-integrating vectors existing. The long-run results revealed that FPI has an appreciating effect on the exchange rate in South Africa. Based on the results, policy recommendations are suggested. , Thesis (MCom) (Economics) -- University of Fort Hare, 2021.
- Full Text:
- Date Issued: 2021-09
- Authors: Lindani, Sandiswa
- Date: 2021-09
- Subjects: Foreign exchange rates , Exchange rate pass-through , Capital movements
- Language: English
- Type: Master's/ theses , text
- Identifier: http://hdl.handle.net/10353/20417 , vital:45664
- Description: The neoclassical theory suggests that free flow of external capital should be equilibrating and thereby facilitating smoothening of an economy's consumption or production patterns. Based on this background, this study empirically examines the extent to which the different forms of capital flows amongst other variables influence the real exchange rate in South Africa for the period 1980 to 2019, using annual data. Estimation techniques employed include the Johansen Co-integration Approach and the Vector error correction model (VECM). The variables were found to be co-integrated, with six co-integrating vectors existing. The long-run results revealed that FPI has an appreciating effect on the exchange rate in South Africa. Based on the results, policy recommendations are suggested. , Thesis (MCom) (Economics) -- University of Fort Hare, 2021.
- Full Text:
- Date Issued: 2021-09
Caregivers’ Knowledge, Attitude and Practices (KAP) of breastfeeding and prevention of mother-to-child transmission (pmtct) in Nelson Mandela Bay Health District: impact of a community dialogue intervention
- Authors: Barnard, Marné
- Date: 2021-04
- Subjects: Breastfeeding -- Transmission -- South Africa -- Nelson Mandela Bay Municipality -- Prevention , Communicable diseases -- South Africa -- Nelson Mandela Bay Municipality -- Prevention
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/50988 , vital:43196
- Description: Background: Globally, childhood mortality remains high with 5.3 million deaths occurring in children under the age of five years in 2018. Of these deaths, 43 000 were children from South Africa, with malnutrition being a significant risk factor contributing to disease and death. Breastfeeding has many benefits and reduces childhood mortality. Current primary health care strategies in South Africa are not having an adequate impact on breastfeeding rates, therefore appropriate messages need to be targeted at the community in order to improve breastfeeding rates. Participatory approaches have been identified as an effective tool to promote child health. Community dialogues led by skilled Early Childhood Development (ECD) mentors from the community might be key in order to improve caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and Prevention of Mother to Child Transmission of HIV (PMTCT). Aim : The aim of the study was to explore the potential effects of community dialogues, led by ECD mentors, on caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and prevention of mother-to-child transmission of HIV (PMTCT). Methods: Quasi-experimental design was used to compare the KAP of caregivers in relation to breastfeeding and PMTCT. Convenience sampling was used to include 63 caregivers who participated in the umbrella project (mixed-methods) and 61 caregivers who did not participate in the project. Ethics approval was obtained from the Nelson Mandela University Faculty Postgraduate Studies Committee. Participants had to provide written informed consent. A skilled interviewer used a structured questionnaire to assess the KAP of all participants. Data were captured on an Excel spreadsheet and analysed in collaboration with a statistician, who analysed the data using Microsoft Excel. Descriptive statistics were applied to numerical data, while frequency distributions were applied to categorical data. Correlations were calculated to describe the degree of relationship between two variables. Inferential statistic tests, conducted at a significance level of 0,05, were used for hypothesis testing. The t-test and analysis of variance (ANOVA) were used in tests involving mean values. Scheffé’s test was used in ANOVA to determine between which of the groups a statistically significant difference was found. Results: In the intervention group more than double the number participants (35%; n=22) had a good knowledge of breastfeeding when compared to participants (15%; n=9) in the control group. Additionally, the intervention group participants displayed better attitudes towards the RtHB (p=0.02). However, control group participants showed better knowledge towards the HIV (p=0.01). Finally, results suggest that in the intervention group, participants (33%; n=21) were more likely to have a good total KAP score in relation to breastfeeding and PMTCT when compared to participants (11%; n=7) in the control group. In the intervention group, better knowledge correlated with good practices relating to the RtHB (r=0.27). However, in the control group, positive attitudes correlated with better practices relating to HIV (r=0.29). Younger intervention group participants displayed better breastfeeding related practices (p=0.011). Additionally, younger control group participants displayed better knowledge of breastfeeding (p=0.001). Intervention group participants whose choices were influenced by their husband/ partner (p =0.016) and/ or radio/ magazines/ newspapers (p=0.004) had positive attitudes towards HIV. Control group participants whose choices were influenced by their other family members (p=0.043) and/ or clinic staff (p=0.016) had good knowledge of sharing their knowledge. Conclusions and recommendations: The intervention group displayed a better knowledge of breastfeeding as well as more desirable attitudes towards the RtHB, when compared to the control group. However, the control group displayed better knowledge of HIV, which could be due to the control group having more younger participants when compared to the intervention group. The community dialogue intervention had a positive impact on caregivers KAP relating to breastfeeding and PMTCT, which included all the different categories combined. In order to improve the Sakha Esethu intervention programme, managers need to provide guidance to the mentors on how to initiate conversations regarding HIV and discuss the stigma associated with HIV and breastfeeding. Results showed that including grandmothers and husbands or partners in the target population will benefit the intervention. Additionally, the use of radio needs to be considered especially for discussions regarding HIV. Lastly, sustainability needs to be improved due to many participants not remembering certain detail regarding topics that was discussed. , Thesis (MSc) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Barnard, Marné
- Date: 2021-04
- Subjects: Breastfeeding -- Transmission -- South Africa -- Nelson Mandela Bay Municipality -- Prevention , Communicable diseases -- South Africa -- Nelson Mandela Bay Municipality -- Prevention
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/50988 , vital:43196
- Description: Background: Globally, childhood mortality remains high with 5.3 million deaths occurring in children under the age of five years in 2018. Of these deaths, 43 000 were children from South Africa, with malnutrition being a significant risk factor contributing to disease and death. Breastfeeding has many benefits and reduces childhood mortality. Current primary health care strategies in South Africa are not having an adequate impact on breastfeeding rates, therefore appropriate messages need to be targeted at the community in order to improve breastfeeding rates. Participatory approaches have been identified as an effective tool to promote child health. Community dialogues led by skilled Early Childhood Development (ECD) mentors from the community might be key in order to improve caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and Prevention of Mother to Child Transmission of HIV (PMTCT). Aim : The aim of the study was to explore the potential effects of community dialogues, led by ECD mentors, on caregivers’ knowledge, attitudes and practices (KAP) in relation to breastfeeding and prevention of mother-to-child transmission of HIV (PMTCT). Methods: Quasi-experimental design was used to compare the KAP of caregivers in relation to breastfeeding and PMTCT. Convenience sampling was used to include 63 caregivers who participated in the umbrella project (mixed-methods) and 61 caregivers who did not participate in the project. Ethics approval was obtained from the Nelson Mandela University Faculty Postgraduate Studies Committee. Participants had to provide written informed consent. A skilled interviewer used a structured questionnaire to assess the KAP of all participants. Data were captured on an Excel spreadsheet and analysed in collaboration with a statistician, who analysed the data using Microsoft Excel. Descriptive statistics were applied to numerical data, while frequency distributions were applied to categorical data. Correlations were calculated to describe the degree of relationship between two variables. Inferential statistic tests, conducted at a significance level of 0,05, were used for hypothesis testing. The t-test and analysis of variance (ANOVA) were used in tests involving mean values. Scheffé’s test was used in ANOVA to determine between which of the groups a statistically significant difference was found. Results: In the intervention group more than double the number participants (35%; n=22) had a good knowledge of breastfeeding when compared to participants (15%; n=9) in the control group. Additionally, the intervention group participants displayed better attitudes towards the RtHB (p=0.02). However, control group participants showed better knowledge towards the HIV (p=0.01). Finally, results suggest that in the intervention group, participants (33%; n=21) were more likely to have a good total KAP score in relation to breastfeeding and PMTCT when compared to participants (11%; n=7) in the control group. In the intervention group, better knowledge correlated with good practices relating to the RtHB (r=0.27). However, in the control group, positive attitudes correlated with better practices relating to HIV (r=0.29). Younger intervention group participants displayed better breastfeeding related practices (p=0.011). Additionally, younger control group participants displayed better knowledge of breastfeeding (p=0.001). Intervention group participants whose choices were influenced by their husband/ partner (p =0.016) and/ or radio/ magazines/ newspapers (p=0.004) had positive attitudes towards HIV. Control group participants whose choices were influenced by their other family members (p=0.043) and/ or clinic staff (p=0.016) had good knowledge of sharing their knowledge. Conclusions and recommendations: The intervention group displayed a better knowledge of breastfeeding as well as more desirable attitudes towards the RtHB, when compared to the control group. However, the control group displayed better knowledge of HIV, which could be due to the control group having more younger participants when compared to the intervention group. The community dialogue intervention had a positive impact on caregivers KAP relating to breastfeeding and PMTCT, which included all the different categories combined. In order to improve the Sakha Esethu intervention programme, managers need to provide guidance to the mentors on how to initiate conversations regarding HIV and discuss the stigma associated with HIV and breastfeeding. Results showed that including grandmothers and husbands or partners in the target population will benefit the intervention. Additionally, the use of radio needs to be considered especially for discussions regarding HIV. Lastly, sustainability needs to be improved due to many participants not remembering certain detail regarding topics that was discussed. , Thesis (MSc) -- Faculty of Health Sciences, 2021
- Full Text:
- Date Issued: 2021-04
Chemical composition and in vitro ruminal degradation of diets containing Vachellia karro leaf meal and their effect on physiological responses of indigenous Xhosa lop-eared goats
- Authors: Mfisi, Khanyisa
- Date: 2021-09
- Subjects: Ruminants--Feeding and feeds , Animal nutrition
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20719 , vital:46460
- Description: The objective of this study was to determine the chemical composition and in vitro ruminal dry matter degradation (DMD) of Vachellia karro leaf meal-containing diets and their effect on feed intake and physiological responses of indigenous Xhosa lop-eared goats. Experimental diets containing 0 percent (Treatment 1), 15 percent (Treatment 2) and 30 percent (Treatment 3) Vachellia karroo leaf meal were mixed with grass hay and crushed maize. Samples of each diet were analysed for chemical composition and in vitro ruminal DM degradability. A total of 18 eight-month-old indigenous Xhosa lop-eared goats, initially weighing 20.81 ± 1.83 kg were randomly assigned to each diet in a Completely Randomized Design. The goats were confined in individual well-ventilated roofed pens and each dietary treatment had a total of 6 goats. The feed was offered at a rate of 3 percent of live weight twice a day, at 08:00 in the morning and at 16:00 in the afternoon. The experimental feeding trial lasted for 80 days, where 10 days was allowed for adaptation. Goat live weight, Average daily feed intake (ADFI), and Body condition scores (BCS) were recorded weekly FAMACHA scores and fecal eggs counts were also determined weekly. Blood samples were collected via vein puncture on the last day of the feeding trial for determination of glucose, total cholesterol, albumin and blood urea. Significantly higher (P < 0.05) crude protein (CP) and condensed tannins (CT) levels were observed in Vachellia karroo containing diets., while acid detergent fibre (ADF) and neutral detergent fibre (NDF) were significantly higher (P < 0.05) in the control diet. During early hours of incubation, DMD was similar (P > 0.05) across all dietary treatments. At 12 to 72 hours, the DMD was significantly higher (P < 0.05) in Vachellia karroo containing diets except at 48 hours. Correlation between DMD and NDF, ADF and fat was only visible at 24 hours. Fibre (ADF and NDF) were negatively related to DMD, while fat was positively related. A positive correlation was observed between DMD and DM, CP and ADFI. The body weight and BCS of goats was similar (P > 0.05) in all dietary treatments (P > 0.05). The ADFI was significantly higher (P < 0.05) in Vachellia karroo supplemented goats. Faecal egg count and FAMACHA scores were significantly higher (P< 0.05) in the control group compared to the Vachellia karroo supplemented treatments; while glucose levels were significantly higher in goats supplemented with Vachellia karroo compared to non-supplemented goats. Cholesterol levels were similar (P > 0.05) across all experimental groups. Goats consuming 30 percent of Vachellia karroo leaf meal had higher (P < 0.05) creatinine levels than the non-supplemented treatment. Urea levels were significantly higher (P < 0.05) in goats fed 30 percent of Vachellia karroo leaf meal than other treatments. It was concluded that the inclusion of Vachellia karroo leaves improves diet quality, diet degradability, average daily feed intake and physiological responses. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Mfisi, Khanyisa
- Date: 2021-09
- Subjects: Ruminants--Feeding and feeds , Animal nutrition
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20719 , vital:46460
- Description: The objective of this study was to determine the chemical composition and in vitro ruminal dry matter degradation (DMD) of Vachellia karro leaf meal-containing diets and their effect on feed intake and physiological responses of indigenous Xhosa lop-eared goats. Experimental diets containing 0 percent (Treatment 1), 15 percent (Treatment 2) and 30 percent (Treatment 3) Vachellia karroo leaf meal were mixed with grass hay and crushed maize. Samples of each diet were analysed for chemical composition and in vitro ruminal DM degradability. A total of 18 eight-month-old indigenous Xhosa lop-eared goats, initially weighing 20.81 ± 1.83 kg were randomly assigned to each diet in a Completely Randomized Design. The goats were confined in individual well-ventilated roofed pens and each dietary treatment had a total of 6 goats. The feed was offered at a rate of 3 percent of live weight twice a day, at 08:00 in the morning and at 16:00 in the afternoon. The experimental feeding trial lasted for 80 days, where 10 days was allowed for adaptation. Goat live weight, Average daily feed intake (ADFI), and Body condition scores (BCS) were recorded weekly FAMACHA scores and fecal eggs counts were also determined weekly. Blood samples were collected via vein puncture on the last day of the feeding trial for determination of glucose, total cholesterol, albumin and blood urea. Significantly higher (P < 0.05) crude protein (CP) and condensed tannins (CT) levels were observed in Vachellia karroo containing diets., while acid detergent fibre (ADF) and neutral detergent fibre (NDF) were significantly higher (P < 0.05) in the control diet. During early hours of incubation, DMD was similar (P > 0.05) across all dietary treatments. At 12 to 72 hours, the DMD was significantly higher (P < 0.05) in Vachellia karroo containing diets except at 48 hours. Correlation between DMD and NDF, ADF and fat was only visible at 24 hours. Fibre (ADF and NDF) were negatively related to DMD, while fat was positively related. A positive correlation was observed between DMD and DM, CP and ADFI. The body weight and BCS of goats was similar (P > 0.05) in all dietary treatments (P > 0.05). The ADFI was significantly higher (P < 0.05) in Vachellia karroo supplemented goats. Faecal egg count and FAMACHA scores were significantly higher (P< 0.05) in the control group compared to the Vachellia karroo supplemented treatments; while glucose levels were significantly higher in goats supplemented with Vachellia karroo compared to non-supplemented goats. Cholesterol levels were similar (P > 0.05) across all experimental groups. Goats consuming 30 percent of Vachellia karroo leaf meal had higher (P < 0.05) creatinine levels than the non-supplemented treatment. Urea levels were significantly higher (P < 0.05) in goats fed 30 percent of Vachellia karroo leaf meal than other treatments. It was concluded that the inclusion of Vachellia karroo leaves improves diet quality, diet degradability, average daily feed intake and physiological responses. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Chemical evaluation, in vitro ruminal dry matter (DM) degradability and ruminal biological activity of common browse tree fruits of Alice
- Authors: Sondzaba, Bavuyise
- Date: 2020-01
- Subjects: Ruminants
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20882 , vital:46695
- Description: The objective of this study was to investigate the effect of species variations on chemical composition and in vitro ruminal degradability of Vachellia karroo, Grewia Occidentalis, Diospyros Lycioides and Ziziphus Mucronata fruits harvested from communally grazed lands in Alice, Eastern Cape Province, South Africa. The browse fruits were collected between February and May and analysed for crude protein (CP), dry matter (DM), ash, neutral detergent fibre (NDF), acid detergent fibre (ADF), organic matter (OM) and acid detergent lignin (ADL), condensed tannins (CT), macro minerals (Calcium (Ca), Magnesium (Mg), Potassium (K), Sodium (Na) and Phosphorus(P)) and micro minerals (Ion (Fe), Zinc (Zn), Manganese (Mn) and Copper (Cu)). There was significant variation (P<0.05) in chemical composition between browse fruits. The CP content differed significantly amongst the browse fruits, but all contained enough to meet the maintenance requirements of ruminants. Fruits from V. Karroo had higher (P<0.05) CP (182.26 g/kg DM), DM (896.50 g/kg DM) and ash (55.60 g/kg DM) contents compared to other browse species, while D. Lycioides fruits had higher (P<0.05) OM (951.60 g/kg DM) content than other browse fruits. Fruits harvested from G. Occidentalis had higher (P<0.05) ADF (513.27 g/kg DM), NDF (577.22 g/kg DM) and ADL (223.20 g/kg DM) content compared to other browse fruits. Fruits from V. Karroo had higher (P<0.05) CT (269.33 g/kg DM) content followed by Z. Mucronata (187.46 g/kg DM), while D. Lycioide (54.22 g/kg DM) and G. Occidentali (66.65 g/kg DM) had the same CT contents (P>0.05). There was a significant variation between browse species in macro and micro mineral contents. Fruits from G. Occindelis (43.93 g/kg DM) and V. Karroo (43.44 g/kg DM) had higher (P<0.05) Ca content compared to Z. Mucronata (24.06 g/kg DM) and D. Lycioides (20.06 g/kg DM). Ziziphus Muronata had higher (P<0.05) P (1.96 g/kg DM) content than other browse fruits. Fruits form V.karroo had higher Mg (4.75 g/kg DM), K (11.50 g/kg DM) and Na 3888 g/kg DM) content compared to other browse species. Fruits from V. Karroo showed significantly higher micro mineral content compared to other species. In terms of dry matter degradability (DMD), incubation time had an apparent effect on degradability of browse fruits except at 4 hours. The DM degradability increased with the increase of time and after 48 hours DMD was found to be satisfactory since it was above the prescribed 40 to 50 percent. However, the presence of condensed tannins had an effect on ruminal degradability of browse fruits. It was concluded that browse fruits have the potential to be used as protein supplements during the dry season since they contain moderate levels of crude protein. However, caution should be taken when feeding these browse fruits since they contain condensed tannins that affect rumen degradability. , Thesis (MSc) (Chemistry) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2020-01
- Authors: Sondzaba, Bavuyise
- Date: 2020-01
- Subjects: Ruminants
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20882 , vital:46695
- Description: The objective of this study was to investigate the effect of species variations on chemical composition and in vitro ruminal degradability of Vachellia karroo, Grewia Occidentalis, Diospyros Lycioides and Ziziphus Mucronata fruits harvested from communally grazed lands in Alice, Eastern Cape Province, South Africa. The browse fruits were collected between February and May and analysed for crude protein (CP), dry matter (DM), ash, neutral detergent fibre (NDF), acid detergent fibre (ADF), organic matter (OM) and acid detergent lignin (ADL), condensed tannins (CT), macro minerals (Calcium (Ca), Magnesium (Mg), Potassium (K), Sodium (Na) and Phosphorus(P)) and micro minerals (Ion (Fe), Zinc (Zn), Manganese (Mn) and Copper (Cu)). There was significant variation (P<0.05) in chemical composition between browse fruits. The CP content differed significantly amongst the browse fruits, but all contained enough to meet the maintenance requirements of ruminants. Fruits from V. Karroo had higher (P<0.05) CP (182.26 g/kg DM), DM (896.50 g/kg DM) and ash (55.60 g/kg DM) contents compared to other browse species, while D. Lycioides fruits had higher (P<0.05) OM (951.60 g/kg DM) content than other browse fruits. Fruits harvested from G. Occidentalis had higher (P<0.05) ADF (513.27 g/kg DM), NDF (577.22 g/kg DM) and ADL (223.20 g/kg DM) content compared to other browse fruits. Fruits from V. Karroo had higher (P<0.05) CT (269.33 g/kg DM) content followed by Z. Mucronata (187.46 g/kg DM), while D. Lycioide (54.22 g/kg DM) and G. Occidentali (66.65 g/kg DM) had the same CT contents (P>0.05). There was a significant variation between browse species in macro and micro mineral contents. Fruits from G. Occindelis (43.93 g/kg DM) and V. Karroo (43.44 g/kg DM) had higher (P<0.05) Ca content compared to Z. Mucronata (24.06 g/kg DM) and D. Lycioides (20.06 g/kg DM). Ziziphus Muronata had higher (P<0.05) P (1.96 g/kg DM) content than other browse fruits. Fruits form V.karroo had higher Mg (4.75 g/kg DM), K (11.50 g/kg DM) and Na 3888 g/kg DM) content compared to other browse species. Fruits from V. Karroo showed significantly higher micro mineral content compared to other species. In terms of dry matter degradability (DMD), incubation time had an apparent effect on degradability of browse fruits except at 4 hours. The DM degradability increased with the increase of time and after 48 hours DMD was found to be satisfactory since it was above the prescribed 40 to 50 percent. However, the presence of condensed tannins had an effect on ruminal degradability of browse fruits. It was concluded that browse fruits have the potential to be used as protein supplements during the dry season since they contain moderate levels of crude protein. However, caution should be taken when feeding these browse fruits since they contain condensed tannins that affect rumen degradability. , Thesis (MSc) (Chemistry) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2020-01
Development of a Mobile-Based Livestock Breeding Management System for Communal Farmers in Eastern Cape, South Africa
- Authors: Aduradola, Oluwatobi John
- Date: 2021-05
- Subjects: Mobile communication systems , Mobile computing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20600 , vital:46364
- Description: More than a decade ago, South Africa realized the significance of Information and Communication Technology (ICT) and its use in delivering several services; there are still several challenges that block implementing an ICT-based system to deliver services and information better. Since communal development is still one of the country's biggest concerns, ICT is among several advances and views recognized for speeding up communal development and providing for the country's economic growth. This research aimed to investigate and implement ICT tools for communal farmers in the context of information needs and information dissemination. The research concentrated on the technical tools and skills needed to implement an ICT tool for communal farmers and other stakeholders. A prototyping methodology was used in this research in the development of the required prototype. A study was then conducted to understand the present state and the information needs of communal farmers where the questionnaire was administered. The research results demonstrated the critical need for communal farmers to get information timely at their convenience. A mobile database system was then designed and developed that suits communal farmers' nature. This system was an offline android application that assists with their information need and its dissemination, and it allows the sharing of the communal farmer's data to other stakeholders. , Thesis (MSc) (Computer Science)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-05
- Authors: Aduradola, Oluwatobi John
- Date: 2021-05
- Subjects: Mobile communication systems , Mobile computing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20600 , vital:46364
- Description: More than a decade ago, South Africa realized the significance of Information and Communication Technology (ICT) and its use in delivering several services; there are still several challenges that block implementing an ICT-based system to deliver services and information better. Since communal development is still one of the country's biggest concerns, ICT is among several advances and views recognized for speeding up communal development and providing for the country's economic growth. This research aimed to investigate and implement ICT tools for communal farmers in the context of information needs and information dissemination. The research concentrated on the technical tools and skills needed to implement an ICT tool for communal farmers and other stakeholders. A prototyping methodology was used in this research in the development of the required prototype. A study was then conducted to understand the present state and the information needs of communal farmers where the questionnaire was administered. The research results demonstrated the critical need for communal farmers to get information timely at their convenience. A mobile database system was then designed and developed that suits communal farmers' nature. This system was an offline android application that assists with their information need and its dissemination, and it allows the sharing of the communal farmer's data to other stakeholders. , Thesis (MSc) (Computer Science)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-05
Effect of environmental responsible behaviour intervention programme on secondary school learners in environmental education knowledge, attitudes and practices in Nigeria
- Authors: Olagbaju, Oluwaseun Olaitan
- Date: 2021-09
- Subjects: Environmental education , Education, Secondary
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20284 , vital:45647
- Description: The depletion of the environment compelled researchers to investigate Environmental Education issues, the methods of imparting knowledge and the creation of awareness about environmental problems. However, youths have knowledge of environmental concepts but lack the skills, intentions, and action competence to exhibit positive environmentally responsible behaviour. There is, therefore, a need for a change in human behaviour to overcome current environmental problems. This study determines the effect of an environmental responsible behaviour intervention programme on Junior Secondary School learners in environmental education. It also explores the moderating effects of gender and school location. The study adopted a pre-test, post-test, control group and quasi-experimental design. A multi-stage sampling technique was employed. Eight (8) coeducational secondary schools in Ibadan, Nigeria, were selected based on environmental challenges in their immediate environment. There were four (4) schools from Ibadan city (urban area of Ibadan) and four (4) from Ibadan less city (rural area of Ibadan). Seven hypotheses were tested at a 0.05 level of significance. Five research instruments were used for this study. Analysis of Covariance (ANCOVA) was used in testing the seven formulated hypotheses. Estimated Marginal mean analysis was used to determine the source of significant effect of treatment. A significant interaction effect was described using a graphical representation. All hypotheses were tested at P< .05 level of significance. The result reveals that there was a significant main effect of treatment (Environmental responsible behaviour intervention programme on Junior Secondary School learners’ environmental knowledge (F(1,715) = 40.62, p < 0.05, Partial , 2 .054). There was also significant main effect of treatment (environmental responsible behaviour intervention programme on junior secondary school students’ environmental attitude. (F(1,715) = 32.36, p < 0.05. partial 2 = 0.043). The effect of treatment (Environmental responsible behaviour intervention programme) on students’ environmental practices was statistically significant, F(1,715) = 17.02, p < 0.05. partial 2 = 0.023). The findings shows that there was a significant main effect of school location (Urban and Rural) on Junior Secondary School students’ environmental knowledge (F(1,716) = 71.62 p< 0.05. partial 2 =0.091). There was no significant main effect of gender (male / female) F(1,715) =0.27, p> 0.05, partial 2 = 0.000. The findings further shows that there was significant main effect of school location on students’ environmental attitude, (F(1,175) =39.21, p< 0.05 partial 2 = 0.052) Shows that there was no significant main effect of gender on students’ environmental attitude F(1,715) =2.1, p> 0.05. partial 2 =0.003 There was significant main effect of school location on students’ environmental practices, F(1,715) = 17.02, p< 0.05 partial 2 = 0.019 but there was no significant main effect of gender on students’ environmental practices, (F(1,715) = 1.17, p> 0.05. partial 2 = 0.002). Based on these findings, it is recommended that teachers should use an environmental responsible behaviour intervention programme in teaching environmental concepts in the classroom. To support this, group/teamwork should be encouraged amongst students. Students should be allowed to construct knowledge in order to bring about their active participation with a view to making learning realistic and effective and to enable the exhibition of environmentally friendly behaviour. , Thesis (PhD)(Education)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Olagbaju, Oluwaseun Olaitan
- Date: 2021-09
- Subjects: Environmental education , Education, Secondary
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20284 , vital:45647
- Description: The depletion of the environment compelled researchers to investigate Environmental Education issues, the methods of imparting knowledge and the creation of awareness about environmental problems. However, youths have knowledge of environmental concepts but lack the skills, intentions, and action competence to exhibit positive environmentally responsible behaviour. There is, therefore, a need for a change in human behaviour to overcome current environmental problems. This study determines the effect of an environmental responsible behaviour intervention programme on Junior Secondary School learners in environmental education. It also explores the moderating effects of gender and school location. The study adopted a pre-test, post-test, control group and quasi-experimental design. A multi-stage sampling technique was employed. Eight (8) coeducational secondary schools in Ibadan, Nigeria, were selected based on environmental challenges in their immediate environment. There were four (4) schools from Ibadan city (urban area of Ibadan) and four (4) from Ibadan less city (rural area of Ibadan). Seven hypotheses were tested at a 0.05 level of significance. Five research instruments were used for this study. Analysis of Covariance (ANCOVA) was used in testing the seven formulated hypotheses. Estimated Marginal mean analysis was used to determine the source of significant effect of treatment. A significant interaction effect was described using a graphical representation. All hypotheses were tested at P< .05 level of significance. The result reveals that there was a significant main effect of treatment (Environmental responsible behaviour intervention programme on Junior Secondary School learners’ environmental knowledge (F(1,715) = 40.62, p < 0.05, Partial , 2 .054). There was also significant main effect of treatment (environmental responsible behaviour intervention programme on junior secondary school students’ environmental attitude. (F(1,715) = 32.36, p < 0.05. partial 2 = 0.043). The effect of treatment (Environmental responsible behaviour intervention programme) on students’ environmental practices was statistically significant, F(1,715) = 17.02, p < 0.05. partial 2 = 0.023). The findings shows that there was a significant main effect of school location (Urban and Rural) on Junior Secondary School students’ environmental knowledge (F(1,716) = 71.62 p< 0.05. partial 2 =0.091). There was no significant main effect of gender (male / female) F(1,715) =0.27, p> 0.05, partial 2 = 0.000. The findings further shows that there was significant main effect of school location on students’ environmental attitude, (F(1,175) =39.21, p< 0.05 partial 2 = 0.052) Shows that there was no significant main effect of gender on students’ environmental attitude F(1,715) =2.1, p> 0.05. partial 2 =0.003 There was significant main effect of school location on students’ environmental practices, F(1,715) = 17.02, p< 0.05 partial 2 = 0.019 but there was no significant main effect of gender on students’ environmental practices, (F(1,715) = 1.17, p> 0.05. partial 2 = 0.002). Based on these findings, it is recommended that teachers should use an environmental responsible behaviour intervention programme in teaching environmental concepts in the classroom. To support this, group/teamwork should be encouraged amongst students. Students should be allowed to construct knowledge in order to bring about their active participation with a view to making learning realistic and effective and to enable the exhibition of environmentally friendly behaviour. , Thesis (PhD)(Education)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Effect of grazing systems and age on enteric methane production and performance plus grazing system effect on ruminal fermentation parameters of Nguni and Bonsmara beef cows
- Authors: Kayima, Denis
- Date: 2021-09
- Subjects: Grazing , Pastures--Management , Ruminants--Feeding and feeds
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20583 , vital:46153
- Description: Beef breeds of cattle are predominantly raised through extensive grazing and produce the highest levels of enteric methane (CH4) through ruminal fermentation. The objective of this study was to evaluate the effect of breed, grazing, and age on enteric methane production and performance of beef cows as well as effect of breed and grazing system on ruminal fermentation parameters. Two separate studies were conducted; the first study evaluated the effect of grazing system, breed, and age on ruminal enteric methane production and performance of selected beef breeds. The second study evaluated the effect of the grazing system and breed on ruminal fermentation. In the first study, two breeds (Nguni and Bonsmara) raised under two grazing systems (communal and commercial) in a random complete block design were grouped into 3 age categories. Eighty-four cows aged between 24-120 months were randomly selected and grouped by age: group A (young adult cow, n = 7, 24-48 months old), group B (adult, n = 7, 60-80 months old), and group C (old adult, n = 7, 90-120 months) in either grazing system. Data were collected on methane production per day, body condition, and weight used to estimate dry matter intake (DMI), methane per DMI, and methane per body weight. In the second study, 40 cows of the same age group, parity, and physiological status were used. Ten cows per breed (Bonsmara and Nguni) in either grazing system (communal and commercial) were used in a random complete block design. Rumen fluid collection was done by the stomach tube method. Data were collected on ruminal ammonia-nitrogen (NH3-N), pH, Temperature, volatile fatty acids (VFAs). Older animals produced higher methane per head in the order of C > B > A; (P<0.0001). Performance parameters like Body condition score (BCS), body weight (BW), dry matter intake (DMI), and daily methane were higher (P<0.05) in Bonsmara. However, Methane per BW was higher in Nguni (P<0.05), and methane per DMI did not differ among breeds (P>0.05). Body condition, BW, and DMI were higher (P<0.05) in commercial grazing system. Contrary, daily methane production, methane production per DMI, and methane produced per BW were higher in communal grazing. In the second study, Ruminal NH3-N concentration ranged between 69.05 - 96.78 mg/L and was higher (P<0.05) in Nguni. It was also higher (P<0.05) in the commercial grazing system. Ruminal pH, temperature, total VFAs, and certain individual VFAs (iso-butyrate, valeric and iso-valeric) did not differ (P>0.05) among breeds and grazing systems. However, total VFAs were slightly lower in communal grazing (78.87 mmol/L) than commercial grazing (89.80 mmol/L). Acetate, propionate, butyrate proportions, and acetate to propionate ratio did not differ (P>0.05) among breeds but differed (P<0.05) between grazing systems. Acetate (P<0.05) and acetate to propionate ratio (P<0.05) were higher in the communal grazing system, while the reverse was true for propionate (P<0.05) and butyrate (P<0.05). Bonsmara produced more methane than Nguni under similar conditions, with Nguni uniquely producing more methane per BW, indicating host genetics influence. Communal animals and older animals produced more CH4 than commercial and younger cows regardless of the breed, indicating ruminal inefficiencies in very old cows. These results emphasize the idea that host genetics or breed, grazing system, and age influence methane output and performance. Hence, breeding strategies for hard-to-measure traits like methane production need to put all these factors into consideration. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Kayima, Denis
- Date: 2021-09
- Subjects: Grazing , Pastures--Management , Ruminants--Feeding and feeds
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20583 , vital:46153
- Description: Beef breeds of cattle are predominantly raised through extensive grazing and produce the highest levels of enteric methane (CH4) through ruminal fermentation. The objective of this study was to evaluate the effect of breed, grazing, and age on enteric methane production and performance of beef cows as well as effect of breed and grazing system on ruminal fermentation parameters. Two separate studies were conducted; the first study evaluated the effect of grazing system, breed, and age on ruminal enteric methane production and performance of selected beef breeds. The second study evaluated the effect of the grazing system and breed on ruminal fermentation. In the first study, two breeds (Nguni and Bonsmara) raised under two grazing systems (communal and commercial) in a random complete block design were grouped into 3 age categories. Eighty-four cows aged between 24-120 months were randomly selected and grouped by age: group A (young adult cow, n = 7, 24-48 months old), group B (adult, n = 7, 60-80 months old), and group C (old adult, n = 7, 90-120 months) in either grazing system. Data were collected on methane production per day, body condition, and weight used to estimate dry matter intake (DMI), methane per DMI, and methane per body weight. In the second study, 40 cows of the same age group, parity, and physiological status were used. Ten cows per breed (Bonsmara and Nguni) in either grazing system (communal and commercial) were used in a random complete block design. Rumen fluid collection was done by the stomach tube method. Data were collected on ruminal ammonia-nitrogen (NH3-N), pH, Temperature, volatile fatty acids (VFAs). Older animals produced higher methane per head in the order of C > B > A; (P<0.0001). Performance parameters like Body condition score (BCS), body weight (BW), dry matter intake (DMI), and daily methane were higher (P<0.05) in Bonsmara. However, Methane per BW was higher in Nguni (P<0.05), and methane per DMI did not differ among breeds (P>0.05). Body condition, BW, and DMI were higher (P<0.05) in commercial grazing system. Contrary, daily methane production, methane production per DMI, and methane produced per BW were higher in communal grazing. In the second study, Ruminal NH3-N concentration ranged between 69.05 - 96.78 mg/L and was higher (P<0.05) in Nguni. It was also higher (P<0.05) in the commercial grazing system. Ruminal pH, temperature, total VFAs, and certain individual VFAs (iso-butyrate, valeric and iso-valeric) did not differ (P>0.05) among breeds and grazing systems. However, total VFAs were slightly lower in communal grazing (78.87 mmol/L) than commercial grazing (89.80 mmol/L). Acetate, propionate, butyrate proportions, and acetate to propionate ratio did not differ (P>0.05) among breeds but differed (P<0.05) between grazing systems. Acetate (P<0.05) and acetate to propionate ratio (P<0.05) were higher in the communal grazing system, while the reverse was true for propionate (P<0.05) and butyrate (P<0.05). Bonsmara produced more methane than Nguni under similar conditions, with Nguni uniquely producing more methane per BW, indicating host genetics influence. Communal animals and older animals produced more CH4 than commercial and younger cows regardless of the breed, indicating ruminal inefficiencies in very old cows. These results emphasize the idea that host genetics or breed, grazing system, and age influence methane output and performance. Hence, breeding strategies for hard-to-measure traits like methane production need to put all these factors into consideration. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Effect of Mucuna pruriens based-diets on fatty acids composition and sensory attributes of meat from broiler chickens
- Authors: Makhamba, Nasiphi
- Date: 2020-11
- Subjects: Fatty acids , Broilers (Chickens)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20771 , vital:46554
- Description: The current study was conducted to determine the effect of feeding different inclusion levels of processed Mucuna pruriens on fatty acid composition and sensory attributes of broiler chickens. 120 Cob500 broilers were used in the experiment. The experiment was divided into 4 treatment (0, 10, 15 and 20 percent of Mucuna pruriens) groups, each treatment with 6 replicates (5 birds per replicate) in a completely randomized design. The first treatment (0 percent) was used as control against the three different levels of Mucuna pruriens. The birds were reared in a deep litter system until they reached slaughter weight of 2kg. At day 42 a total of 80 birds were slaughtered and breast muscles were sampled for the evaluation of sensory attributes and fatty acid composition. For sensory evaluation meat samples were prepared and boiled at 80°C until the internal temperature reached 82°C, 40 consumers were used to assess the meat samples and give scores according to 9-point hedonic scale. The fatty acid content was determined and recorded using the gas chromatography. Total saturated fatty acid (SFA), total monounsaturated fatty acid (MUFA), and total polyunsaturated fatty acid (PUFA) of the breast meat were not affected by diets. Omega-6 and omega-3 fatty acid were also not influenced by treatment diets. However, breast muscle from broilers fed 20 percent Mucuna pruriens seed meal inclusion had lower Myristic acid. The sensory attributes; aroma, colour, flavour, juiciness, tenderness and acceptability were assessed, dietary treatments affected the juiciness, tenderness and acceptability of meat, the treatments showed no significant difference on colour, aroma and flavour. Different age groups accepted the meat differently, the respondents that were between 18 and 22 years old gave the highest scores to the acceptability of meat than the other age groups, and different age groups also gave different scores in the aroma of meat. Females and males accepted the meat differently, the female respondents gave higher scores on the flavour of meat than males. Different inclusion levels of Mucuna pruriens positively affected fatty acid composition and sensory attributes of broiler chickens without causing any detrimental effects. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2020-11
- Authors: Makhamba, Nasiphi
- Date: 2020-11
- Subjects: Fatty acids , Broilers (Chickens)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20771 , vital:46554
- Description: The current study was conducted to determine the effect of feeding different inclusion levels of processed Mucuna pruriens on fatty acid composition and sensory attributes of broiler chickens. 120 Cob500 broilers were used in the experiment. The experiment was divided into 4 treatment (0, 10, 15 and 20 percent of Mucuna pruriens) groups, each treatment with 6 replicates (5 birds per replicate) in a completely randomized design. The first treatment (0 percent) was used as control against the three different levels of Mucuna pruriens. The birds were reared in a deep litter system until they reached slaughter weight of 2kg. At day 42 a total of 80 birds were slaughtered and breast muscles were sampled for the evaluation of sensory attributes and fatty acid composition. For sensory evaluation meat samples were prepared and boiled at 80°C until the internal temperature reached 82°C, 40 consumers were used to assess the meat samples and give scores according to 9-point hedonic scale. The fatty acid content was determined and recorded using the gas chromatography. Total saturated fatty acid (SFA), total monounsaturated fatty acid (MUFA), and total polyunsaturated fatty acid (PUFA) of the breast meat were not affected by diets. Omega-6 and omega-3 fatty acid were also not influenced by treatment diets. However, breast muscle from broilers fed 20 percent Mucuna pruriens seed meal inclusion had lower Myristic acid. The sensory attributes; aroma, colour, flavour, juiciness, tenderness and acceptability were assessed, dietary treatments affected the juiciness, tenderness and acceptability of meat, the treatments showed no significant difference on colour, aroma and flavour. Different age groups accepted the meat differently, the respondents that were between 18 and 22 years old gave the highest scores to the acceptability of meat than the other age groups, and different age groups also gave different scores in the aroma of meat. Females and males accepted the meat differently, the female respondents gave higher scores on the flavour of meat than males. Different inclusion levels of Mucuna pruriens positively affected fatty acid composition and sensory attributes of broiler chickens without causing any detrimental effects. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2020-11
Evaluation of medicinal potential of Boophone disticha (L.f.) Herb. used by the indigenous people in the Raymond Mhlaba Municipality Eastern Cape
- Authors: Gwayi, Yamkela
- Date: 2021-09
- Subjects: Bulbs (Plants) , Bulbs (Plant anatomy)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20612 , vital:46375
- Description: Boophone disticha (L.f.) Herb. is a bulbous plant native to southern African areas of the continent and spreads to tropical Africa. The plant has been noted in literature for its high toxicity and has a long lineage of its use in traditional medicine for the treatment of various diseases. Due to high demand in the conventional trade market, the plant's conservation status has been shown to be declining. The plant has been reported to cause human poisoning, which can lead to death, but the plant is still administered by an indigenous community to treat various diseases. This study investigated the therapeutic potential of B. disticha used by the people of Raymond Mhlaba municipality to show and verify its use in traditional medicine and how it can be used as a possible drug ingredient. This study was also set out to investigate the anti-mycobacterial activity of the plant for the first time for the B. disticha growing in the Eastern Cape region of South Africa. The phytochemical analysis of B. disticha was carried out on four different plant parts (i.e., roots, leaves, bulb inner and outer scales) and the plant was extracted using methanol and distilled water. The qualitative phytochemical analysis displayed the presence of tannins, flavonoids, phenols and alkaloids in all plant parts for both extraction solvents. Anthraquinones were absent in all the plant extracts. The detected phytochemicals (tannins, flavonoids, phenols and alkaloids) were quantified. The roots had the highest phytochemical content for methanol extract for each phytochemical tested [439.67±1.53 mg/g (QE) flavonoids, 2414.67±1.53 mg/g (GAE) phenols and 527.33±2.08 mg/g (GAE) alkaloids] compared to all the plant parts. Methanol extracts from B. disticha revealed highest phenolic contents (2414.67±1.53 mg/g GAE) for the roots, 1395.33±2.52 mg/g (GAE) for the bulb inner scales, 1560±1 mg/g (GAE) for the leaves and 1550.7±18.9 mg/g (GAE) for the bulb outer scales). The total flavonoid content of the bulb outer scales aqueous extract was not detected. The presence of significant amounts of phytochemical compounds indicates that B. disticha has a higher medicinal value and can be extensively investigated to extract bioactive ingredients that are useful to the society, and that could be sold for higher production than using synthetic drugs with side effects. The antimicrobial activity of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well micro-plates against nine disease-causing bacterial strains (5 Gram-negative and 4 Gram-positive) and three fungal isolates. The results revealed that methanol and aqueous extract of B. disticha demonstrated very good activity, with Gram-positive strains being more sensitive than Gram-negative ones. Boophone disticha aqueous extracts displayed the best activity against Staphylococcus aureus with MIC and MBC values ranging from 0.39 to 0.78 mg/ml. The methanolic extract of B. disticha leaves and outer scales of bulb displayed good activity against Klebsiella pneumoniae at MIC 0.78 mg/ml. The methanolic extract of B. disticha bulb outer scales also displayed good inhibition against Proteus vulgaris at MIC 0.78 mg/ml, with the root extract exhibiting activity against Shigella flexineri (MIC value of 0.078 mg/ml). Concerning antifungal activity, B. disticha extracts showed very poor inhibition properties against the fungal isolates. Anti-mycobacterium potential of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well microtiter plates. The plant parts tested were the roots, bulb inner scales, leaves and the bulb outer scales. The highest activity against Mycobacterium tuberculosis was observed with the root methanol extract at MIC 0.78 mg/ml. The anti-inflammatory properties of B. disticha were investigated using the 5-lypoxygenase (5- LOX) assay. The overall anti-inflammatory activity results for the B. disticha extracts were poor; at low concentrations, the plant displayed negative results. The leaves methanol extracts did show little activity at 0.4 mg/ml. , Thesis (MSc) (Botany) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Gwayi, Yamkela
- Date: 2021-09
- Subjects: Bulbs (Plants) , Bulbs (Plant anatomy)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20612 , vital:46375
- Description: Boophone disticha (L.f.) Herb. is a bulbous plant native to southern African areas of the continent and spreads to tropical Africa. The plant has been noted in literature for its high toxicity and has a long lineage of its use in traditional medicine for the treatment of various diseases. Due to high demand in the conventional trade market, the plant's conservation status has been shown to be declining. The plant has been reported to cause human poisoning, which can lead to death, but the plant is still administered by an indigenous community to treat various diseases. This study investigated the therapeutic potential of B. disticha used by the people of Raymond Mhlaba municipality to show and verify its use in traditional medicine and how it can be used as a possible drug ingredient. This study was also set out to investigate the anti-mycobacterial activity of the plant for the first time for the B. disticha growing in the Eastern Cape region of South Africa. The phytochemical analysis of B. disticha was carried out on four different plant parts (i.e., roots, leaves, bulb inner and outer scales) and the plant was extracted using methanol and distilled water. The qualitative phytochemical analysis displayed the presence of tannins, flavonoids, phenols and alkaloids in all plant parts for both extraction solvents. Anthraquinones were absent in all the plant extracts. The detected phytochemicals (tannins, flavonoids, phenols and alkaloids) were quantified. The roots had the highest phytochemical content for methanol extract for each phytochemical tested [439.67±1.53 mg/g (QE) flavonoids, 2414.67±1.53 mg/g (GAE) phenols and 527.33±2.08 mg/g (GAE) alkaloids] compared to all the plant parts. Methanol extracts from B. disticha revealed highest phenolic contents (2414.67±1.53 mg/g GAE) for the roots, 1395.33±2.52 mg/g (GAE) for the bulb inner scales, 1560±1 mg/g (GAE) for the leaves and 1550.7±18.9 mg/g (GAE) for the bulb outer scales). The total flavonoid content of the bulb outer scales aqueous extract was not detected. The presence of significant amounts of phytochemical compounds indicates that B. disticha has a higher medicinal value and can be extensively investigated to extract bioactive ingredients that are useful to the society, and that could be sold for higher production than using synthetic drugs with side effects. The antimicrobial activity of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well micro-plates against nine disease-causing bacterial strains (5 Gram-negative and 4 Gram-positive) and three fungal isolates. The results revealed that methanol and aqueous extract of B. disticha demonstrated very good activity, with Gram-positive strains being more sensitive than Gram-negative ones. Boophone disticha aqueous extracts displayed the best activity against Staphylococcus aureus with MIC and MBC values ranging from 0.39 to 0.78 mg/ml. The methanolic extract of B. disticha leaves and outer scales of bulb displayed good activity against Klebsiella pneumoniae at MIC 0.78 mg/ml. The methanolic extract of B. disticha bulb outer scales also displayed good inhibition against Proteus vulgaris at MIC 0.78 mg/ml, with the root extract exhibiting activity against Shigella flexineri (MIC value of 0.078 mg/ml). Concerning antifungal activity, B. disticha extracts showed very poor inhibition properties against the fungal isolates. Anti-mycobacterium potential of B. disticha extracts (methanol and distilled water) was evaluated using micro-dilution bioassay in 96-well microtiter plates. The plant parts tested were the roots, bulb inner scales, leaves and the bulb outer scales. The highest activity against Mycobacterium tuberculosis was observed with the root methanol extract at MIC 0.78 mg/ml. The anti-inflammatory properties of B. disticha were investigated using the 5-lypoxygenase (5- LOX) assay. The overall anti-inflammatory activity results for the B. disticha extracts were poor; at low concentrations, the plant displayed negative results. The leaves methanol extracts did show little activity at 0.4 mg/ml. , Thesis (MSc) (Botany) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09