Bioethanol production from waste paper through fungal biotechnology
- Authors: Voigt, Paul George
- Date: 2010
- Subjects: Biomass energy , Cellulose -- Biodegradation , Waste paper -- Recycling , Biomass chemicals -- Economic aspects , Renewable energy sources , Fungi -- Biotechnology , Enzymes -- Biotechnology
- Language: English
- Type: Thesis , MSc , Masters
- Identifier: vital:3861 , http://hdl.handle.net/10962/d1013447
- Description: Bioethanol is likely to be a large contributor to the fuel sector of industry in the near future. Current research trends are geared towards utilizing food crops as substrate for bioethanol fermentation; however, this is the source of much controversy. Utilizing food crops for fuel purposes is anticipated to cause massive food shortages worldwide. Cellulose is the most abundant renewable resource on earth and is subject to a wide array of scientific study in order to utilize the glucose contained within it. Waste paper has a high degree of cellulose associated with it, which makes it an ideal target for cellulose biotechnology with the ultimate end goal of bioethanol production. This study focussed on producing the necessary enzymes to hydrolyse the cellulose found in waste paper and using the sugars produced to produce ethanol. The effects of various printing inks had on the production of sugars and the total envirorunental impact of the effluents produced during the production line were also examined. It was found that the fungus Trichoderma longibrachiatum DSM 769 grown in Mandel's medium with waste newspaper as the sole carbon source at 28 °C for 6 days produced extracellular cellulase enzymes with an activity of 0.203 ± 0.009 FPU.ml⁻¹, significantly higher activity as compared to other paper sources. This extracellular cellulase was used to hydrolyse waste newspaper and office paper, with office paper yielding the highest degree of sugar production with an end concentration of 5.80 ± 0.19 g/1 at 40 °C. Analysis by HPLC showed that although glucose was the major product at 4.35 ± 0.12 g/1, cellobiose was also produced in appreciable amounts (1.97 ± 0.71 g/1). The sugar solution was used as a substrate for Saccharomyces cerevisiae DSM 1333 and ethanol was produced at a level of 1.79 ± 0.26 g/1, the presence of which was confirmed by a 600 MHz NMR spectrum. It was found that cellobiose was not fermented by this strain of S. cerevisiae. Certain components of inks (the PAHs phenanthrene and naphthalene) were found to have a slight inhibitory effect (approximately 15% decrease) on the cellulase enzymes at very high concentrations (approximately 600 μg/1 in aqueous medium), while anthracene had no effect. Whole newsprint ink was shown not to sorb glucose. The environmental analysis of the effluents produced showed that in order for the effluents to be discharged into an aqueous ecosystem they would have to be diluted up to 200 times. They were also shown to have the potential to cause severe machinery damage if reused without proper treatment.
- Full Text:
- Date Issued: 2010
- Authors: Voigt, Paul George
- Date: 2010
- Subjects: Biomass energy , Cellulose -- Biodegradation , Waste paper -- Recycling , Biomass chemicals -- Economic aspects , Renewable energy sources , Fungi -- Biotechnology , Enzymes -- Biotechnology
- Language: English
- Type: Thesis , MSc , Masters
- Identifier: vital:3861 , http://hdl.handle.net/10962/d1013447
- Description: Bioethanol is likely to be a large contributor to the fuel sector of industry in the near future. Current research trends are geared towards utilizing food crops as substrate for bioethanol fermentation; however, this is the source of much controversy. Utilizing food crops for fuel purposes is anticipated to cause massive food shortages worldwide. Cellulose is the most abundant renewable resource on earth and is subject to a wide array of scientific study in order to utilize the glucose contained within it. Waste paper has a high degree of cellulose associated with it, which makes it an ideal target for cellulose biotechnology with the ultimate end goal of bioethanol production. This study focussed on producing the necessary enzymes to hydrolyse the cellulose found in waste paper and using the sugars produced to produce ethanol. The effects of various printing inks had on the production of sugars and the total envirorunental impact of the effluents produced during the production line were also examined. It was found that the fungus Trichoderma longibrachiatum DSM 769 grown in Mandel's medium with waste newspaper as the sole carbon source at 28 °C for 6 days produced extracellular cellulase enzymes with an activity of 0.203 ± 0.009 FPU.ml⁻¹, significantly higher activity as compared to other paper sources. This extracellular cellulase was used to hydrolyse waste newspaper and office paper, with office paper yielding the highest degree of sugar production with an end concentration of 5.80 ± 0.19 g/1 at 40 °C. Analysis by HPLC showed that although glucose was the major product at 4.35 ± 0.12 g/1, cellobiose was also produced in appreciable amounts (1.97 ± 0.71 g/1). The sugar solution was used as a substrate for Saccharomyces cerevisiae DSM 1333 and ethanol was produced at a level of 1.79 ± 0.26 g/1, the presence of which was confirmed by a 600 MHz NMR spectrum. It was found that cellobiose was not fermented by this strain of S. cerevisiae. Certain components of inks (the PAHs phenanthrene and naphthalene) were found to have a slight inhibitory effect (approximately 15% decrease) on the cellulase enzymes at very high concentrations (approximately 600 μg/1 in aqueous medium), while anthracene had no effect. Whole newsprint ink was shown not to sorb glucose. The environmental analysis of the effluents produced showed that in order for the effluents to be discharged into an aqueous ecosystem they would have to be diluted up to 200 times. They were also shown to have the potential to cause severe machinery damage if reused without proper treatment.
- Full Text:
- Date Issued: 2010
The design, management and testing of a solar vehicle's energy strategy
- Authors: Jordaan, Johan
- Date: 2016
- Subjects: Solar energy -- Hybrid systems , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/7428 , vital:21362
- Description: In recent years the interest in implementing solar energy on vehicles (electrical and hybrid) has grown significantly [1]. There are currently limitations in this sector, such as the low energy density (efficiency of conversion) of this source, but it is still a renewable resource and as such, there is a growing interest [1]. A “smart” energy strategy implemented on a solar/electrical vehicle, in order to increase its energy harvesting volume, could enhance the growth of this sector. A tracking algorithm for a solar vehicle’s MPPT (Maximum Power Point Tracker) can be designed to source solar energy very effectively and to increase the speed of finding (tracking) this optimal sourcing point (solar panel voltage and current). Even though there are many different MPPT algorithms, it was decided that most of them were designed for stationary MPPT applications and the dynamics of implementing a MPPT on a vehicle create some unique scenarios. These include: Shadow flicker. This is rhythmic, rapid moving shadows across a solar panel, such as shadows from a line of trees: Rapid changes in solar panel orientation due to the road surface/relief; Rapid changes in panel temperature due to the location of the vehicle. The aim of the research can be divided into three outcomes: 1 Creating a “Smart” energy strategy/control, 2 Implement the new control system on a solar vehicle’s MPPT, and 3 Harvesting maximum energy from solar panels using the new energy strategy. The term “smart” is used to indicate the ability of the MPPT algorithm to be updated and improved based on previous results. A MPPT and scaled solar vehicle is designed and manufactured in order to test the MPPT algorithm. The purpose of using a self-developed experimental setup is to have more control over the system variables as well as having the maximum freedom in setting up the system parameters.
- Full Text:
- Date Issued: 2016
- Authors: Jordaan, Johan
- Date: 2016
- Subjects: Solar energy -- Hybrid systems , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/7428 , vital:21362
- Description: In recent years the interest in implementing solar energy on vehicles (electrical and hybrid) has grown significantly [1]. There are currently limitations in this sector, such as the low energy density (efficiency of conversion) of this source, but it is still a renewable resource and as such, there is a growing interest [1]. A “smart” energy strategy implemented on a solar/electrical vehicle, in order to increase its energy harvesting volume, could enhance the growth of this sector. A tracking algorithm for a solar vehicle’s MPPT (Maximum Power Point Tracker) can be designed to source solar energy very effectively and to increase the speed of finding (tracking) this optimal sourcing point (solar panel voltage and current). Even though there are many different MPPT algorithms, it was decided that most of them were designed for stationary MPPT applications and the dynamics of implementing a MPPT on a vehicle create some unique scenarios. These include: Shadow flicker. This is rhythmic, rapid moving shadows across a solar panel, such as shadows from a line of trees: Rapid changes in solar panel orientation due to the road surface/relief; Rapid changes in panel temperature due to the location of the vehicle. The aim of the research can be divided into three outcomes: 1 Creating a “Smart” energy strategy/control, 2 Implement the new control system on a solar vehicle’s MPPT, and 3 Harvesting maximum energy from solar panels using the new energy strategy. The term “smart” is used to indicate the ability of the MPPT algorithm to be updated and improved based on previous results. A MPPT and scaled solar vehicle is designed and manufactured in order to test the MPPT algorithm. The purpose of using a self-developed experimental setup is to have more control over the system variables as well as having the maximum freedom in setting up the system parameters.
- Full Text:
- Date Issued: 2016
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