Bioethanol production from waste paper through fungal biotechnology
- Authors: Voigt, Paul George
- Date: 2010
- Subjects: Biomass energy , Cellulose -- Biodegradation , Waste paper -- Recycling , Biomass chemicals -- Economic aspects , Renewable energy sources , Fungi -- Biotechnology , Enzymes -- Biotechnology
- Language: English
- Type: Thesis , MSc , Masters
- Identifier: vital:3861 , http://hdl.handle.net/10962/d1013447
- Description: Bioethanol is likely to be a large contributor to the fuel sector of industry in the near future. Current research trends are geared towards utilizing food crops as substrate for bioethanol fermentation; however, this is the source of much controversy. Utilizing food crops for fuel purposes is anticipated to cause massive food shortages worldwide. Cellulose is the most abundant renewable resource on earth and is subject to a wide array of scientific study in order to utilize the glucose contained within it. Waste paper has a high degree of cellulose associated with it, which makes it an ideal target for cellulose biotechnology with the ultimate end goal of bioethanol production. This study focussed on producing the necessary enzymes to hydrolyse the cellulose found in waste paper and using the sugars produced to produce ethanol. The effects of various printing inks had on the production of sugars and the total envirorunental impact of the effluents produced during the production line were also examined. It was found that the fungus Trichoderma longibrachiatum DSM 769 grown in Mandel's medium with waste newspaper as the sole carbon source at 28 °C for 6 days produced extracellular cellulase enzymes with an activity of 0.203 ± 0.009 FPU.ml⁻¹, significantly higher activity as compared to other paper sources. This extracellular cellulase was used to hydrolyse waste newspaper and office paper, with office paper yielding the highest degree of sugar production with an end concentration of 5.80 ± 0.19 g/1 at 40 °C. Analysis by HPLC showed that although glucose was the major product at 4.35 ± 0.12 g/1, cellobiose was also produced in appreciable amounts (1.97 ± 0.71 g/1). The sugar solution was used as a substrate for Saccharomyces cerevisiae DSM 1333 and ethanol was produced at a level of 1.79 ± 0.26 g/1, the presence of which was confirmed by a 600 MHz NMR spectrum. It was found that cellobiose was not fermented by this strain of S. cerevisiae. Certain components of inks (the PAHs phenanthrene and naphthalene) were found to have a slight inhibitory effect (approximately 15% decrease) on the cellulase enzymes at very high concentrations (approximately 600 μg/1 in aqueous medium), while anthracene had no effect. Whole newsprint ink was shown not to sorb glucose. The environmental analysis of the effluents produced showed that in order for the effluents to be discharged into an aqueous ecosystem they would have to be diluted up to 200 times. They were also shown to have the potential to cause severe machinery damage if reused without proper treatment.
- Full Text:
- Date Issued: 2010
- Authors: Voigt, Paul George
- Date: 2010
- Subjects: Biomass energy , Cellulose -- Biodegradation , Waste paper -- Recycling , Biomass chemicals -- Economic aspects , Renewable energy sources , Fungi -- Biotechnology , Enzymes -- Biotechnology
- Language: English
- Type: Thesis , MSc , Masters
- Identifier: vital:3861 , http://hdl.handle.net/10962/d1013447
- Description: Bioethanol is likely to be a large contributor to the fuel sector of industry in the near future. Current research trends are geared towards utilizing food crops as substrate for bioethanol fermentation; however, this is the source of much controversy. Utilizing food crops for fuel purposes is anticipated to cause massive food shortages worldwide. Cellulose is the most abundant renewable resource on earth and is subject to a wide array of scientific study in order to utilize the glucose contained within it. Waste paper has a high degree of cellulose associated with it, which makes it an ideal target for cellulose biotechnology with the ultimate end goal of bioethanol production. This study focussed on producing the necessary enzymes to hydrolyse the cellulose found in waste paper and using the sugars produced to produce ethanol. The effects of various printing inks had on the production of sugars and the total envirorunental impact of the effluents produced during the production line were also examined. It was found that the fungus Trichoderma longibrachiatum DSM 769 grown in Mandel's medium with waste newspaper as the sole carbon source at 28 °C for 6 days produced extracellular cellulase enzymes with an activity of 0.203 ± 0.009 FPU.ml⁻¹, significantly higher activity as compared to other paper sources. This extracellular cellulase was used to hydrolyse waste newspaper and office paper, with office paper yielding the highest degree of sugar production with an end concentration of 5.80 ± 0.19 g/1 at 40 °C. Analysis by HPLC showed that although glucose was the major product at 4.35 ± 0.12 g/1, cellobiose was also produced in appreciable amounts (1.97 ± 0.71 g/1). The sugar solution was used as a substrate for Saccharomyces cerevisiae DSM 1333 and ethanol was produced at a level of 1.79 ± 0.26 g/1, the presence of which was confirmed by a 600 MHz NMR spectrum. It was found that cellobiose was not fermented by this strain of S. cerevisiae. Certain components of inks (the PAHs phenanthrene and naphthalene) were found to have a slight inhibitory effect (approximately 15% decrease) on the cellulase enzymes at very high concentrations (approximately 600 μg/1 in aqueous medium), while anthracene had no effect. Whole newsprint ink was shown not to sorb glucose. The environmental analysis of the effluents produced showed that in order for the effluents to be discharged into an aqueous ecosystem they would have to be diluted up to 200 times. They were also shown to have the potential to cause severe machinery damage if reused without proper treatment.
- Full Text:
- Date Issued: 2010
Development of a bench scale single batch biomass to liquid fuel facility
- Authors: Zhang, Yusheng
- Date: 2014
- Subjects: Biomass energy , Renewable energy sources , Energy conversion , Electric power production
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: http://hdl.handle.net/10353/811 , vital:26499 , Biomass energy , Renewable energy sources , Energy conversion , Electric power production
- Description: The research described in this dissertation was motivated by the global demand for energy that is not dependent on coal, oil, natural gas and other non-renewable fossil fuels. The technology used in this project is related to the use of biomass to produce a viable alternative to conventional sources of fuel. A bench scale biomass to liquid (BTL) facility was built and tested. This produced results confirming the feasibility of the BTL process. The findings of the pilot study outlined in this dissertation justified the conclusion that the next step will be to expand the capacity and productivity of the BTL pilot plant to an industrial scale. Biomass comes from a variety of renewable sources that are readily available. In this case, the material used in the fixed bed biomass gasification facility to generate wood gas was agricultural and forestry waste, such as straw and wood chips. The gasifier had the capacity to produce up to 10 cubic metres/hr of gas with a carbon monoxide and hydrogen content of between 20–40% by volume, when it was operated at ambient pressure and with air as the oxidizer. The gas, produced at a temperature above 700º C, was cooled in a quench/water scrubber in order to remove most of the mechanical impurities (tars and water-soluble inorganic particles), condensed and dried with corn cobs before being compressed in cylinders at over 100 bar (g) for use in the Fischer-Tropsch Synthesis (FTS). The syngas was subjected further to a series of refining processes which included removal of sulphur and oxygen. The sulphur removal technology chosen entailed applying modified activated carbon to adsorb H2S with the help of hydrolysis in order to convert organic sulphur impurities into H2S which reduced the sulphur content of the gas to less than 5 ppbv. Supported cobalt catalyst (100 grams), were loaded into a single-tube fixed bed FT reactor with an inner diameter of 50 mm. The reactor was fitted with a heating jacket through which, heated oil ran to cool the reactor during a normal reaction occurring at < 250 ºC, while nitrogen was used in the heating jacket during reduction, which occurred at temperatures up ~ 350 ºC. The FTS reaction was carried out at different pressures and temperatures. Liquid and wax products were produced from the facility. The properties of the liquid and solid hydrocarbons produced were found to be the same as FT products from other feed stocks, such as natural gas and coal.
- Full Text:
- Date Issued: 2014
- Authors: Zhang, Yusheng
- Date: 2014
- Subjects: Biomass energy , Renewable energy sources , Energy conversion , Electric power production
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: http://hdl.handle.net/10353/811 , vital:26499 , Biomass energy , Renewable energy sources , Energy conversion , Electric power production
- Description: The research described in this dissertation was motivated by the global demand for energy that is not dependent on coal, oil, natural gas and other non-renewable fossil fuels. The technology used in this project is related to the use of biomass to produce a viable alternative to conventional sources of fuel. A bench scale biomass to liquid (BTL) facility was built and tested. This produced results confirming the feasibility of the BTL process. The findings of the pilot study outlined in this dissertation justified the conclusion that the next step will be to expand the capacity and productivity of the BTL pilot plant to an industrial scale. Biomass comes from a variety of renewable sources that are readily available. In this case, the material used in the fixed bed biomass gasification facility to generate wood gas was agricultural and forestry waste, such as straw and wood chips. The gasifier had the capacity to produce up to 10 cubic metres/hr of gas with a carbon monoxide and hydrogen content of between 20–40% by volume, when it was operated at ambient pressure and with air as the oxidizer. The gas, produced at a temperature above 700º C, was cooled in a quench/water scrubber in order to remove most of the mechanical impurities (tars and water-soluble inorganic particles), condensed and dried with corn cobs before being compressed in cylinders at over 100 bar (g) for use in the Fischer-Tropsch Synthesis (FTS). The syngas was subjected further to a series of refining processes which included removal of sulphur and oxygen. The sulphur removal technology chosen entailed applying modified activated carbon to adsorb H2S with the help of hydrolysis in order to convert organic sulphur impurities into H2S which reduced the sulphur content of the gas to less than 5 ppbv. Supported cobalt catalyst (100 grams), were loaded into a single-tube fixed bed FT reactor with an inner diameter of 50 mm. The reactor was fitted with a heating jacket through which, heated oil ran to cool the reactor during a normal reaction occurring at < 250 ºC, while nitrogen was used in the heating jacket during reduction, which occurred at temperatures up ~ 350 ºC. The FTS reaction was carried out at different pressures and temperatures. Liquid and wax products were produced from the facility. The properties of the liquid and solid hydrocarbons produced were found to be the same as FT products from other feed stocks, such as natural gas and coal.
- Full Text:
- Date Issued: 2014
The design, management and testing of a solar vehicle's energy strategy
- Authors: Jordaan, Johan
- Date: 2016
- Subjects: Solar energy -- Hybrid systems , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/7428 , vital:21362
- Description: In recent years the interest in implementing solar energy on vehicles (electrical and hybrid) has grown significantly [1]. There are currently limitations in this sector, such as the low energy density (efficiency of conversion) of this source, but it is still a renewable resource and as such, there is a growing interest [1]. A “smart” energy strategy implemented on a solar/electrical vehicle, in order to increase its energy harvesting volume, could enhance the growth of this sector. A tracking algorithm for a solar vehicle’s MPPT (Maximum Power Point Tracker) can be designed to source solar energy very effectively and to increase the speed of finding (tracking) this optimal sourcing point (solar panel voltage and current). Even though there are many different MPPT algorithms, it was decided that most of them were designed for stationary MPPT applications and the dynamics of implementing a MPPT on a vehicle create some unique scenarios. These include: Shadow flicker. This is rhythmic, rapid moving shadows across a solar panel, such as shadows from a line of trees: Rapid changes in solar panel orientation due to the road surface/relief; Rapid changes in panel temperature due to the location of the vehicle. The aim of the research can be divided into three outcomes: 1 Creating a “Smart” energy strategy/control, 2 Implement the new control system on a solar vehicle’s MPPT, and 3 Harvesting maximum energy from solar panels using the new energy strategy. The term “smart” is used to indicate the ability of the MPPT algorithm to be updated and improved based on previous results. A MPPT and scaled solar vehicle is designed and manufactured in order to test the MPPT algorithm. The purpose of using a self-developed experimental setup is to have more control over the system variables as well as having the maximum freedom in setting up the system parameters.
- Full Text:
- Date Issued: 2016
- Authors: Jordaan, Johan
- Date: 2016
- Subjects: Solar energy -- Hybrid systems , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/7428 , vital:21362
- Description: In recent years the interest in implementing solar energy on vehicles (electrical and hybrid) has grown significantly [1]. There are currently limitations in this sector, such as the low energy density (efficiency of conversion) of this source, but it is still a renewable resource and as such, there is a growing interest [1]. A “smart” energy strategy implemented on a solar/electrical vehicle, in order to increase its energy harvesting volume, could enhance the growth of this sector. A tracking algorithm for a solar vehicle’s MPPT (Maximum Power Point Tracker) can be designed to source solar energy very effectively and to increase the speed of finding (tracking) this optimal sourcing point (solar panel voltage and current). Even though there are many different MPPT algorithms, it was decided that most of them were designed for stationary MPPT applications and the dynamics of implementing a MPPT on a vehicle create some unique scenarios. These include: Shadow flicker. This is rhythmic, rapid moving shadows across a solar panel, such as shadows from a line of trees: Rapid changes in solar panel orientation due to the road surface/relief; Rapid changes in panel temperature due to the location of the vehicle. The aim of the research can be divided into three outcomes: 1 Creating a “Smart” energy strategy/control, 2 Implement the new control system on a solar vehicle’s MPPT, and 3 Harvesting maximum energy from solar panels using the new energy strategy. The term “smart” is used to indicate the ability of the MPPT algorithm to be updated and improved based on previous results. A MPPT and scaled solar vehicle is designed and manufactured in order to test the MPPT algorithm. The purpose of using a self-developed experimental setup is to have more control over the system variables as well as having the maximum freedom in setting up the system parameters.
- Full Text:
- Date Issued: 2016
The impact of solar water heaters on sustainable development
- Authors: Mbeng, Regina Nso
- Date: 2014
- Subjects: Solar water heaters , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9238 , http://hdl.handle.net/10948/d1021103
- Description: In this present world order of growing information, communication and technological advancement, it is primordial that climate change adaptation and mitigation efforts seeks to make people resilient to inevitable climate inconsistency through the exploitation and development of renewable energy sources. Though climate impacts are global, the population most at risk is predominantly from developing countries, particularly poor communities who have experienced multi-layered threats from floods, droughts and energy supply, just to mention a few. This explains why sustainable development is at the heart of all development trajectories in the world today, specifically the post-2015 development tenets. Climate change adaptation and mitigation requires international collaboration from all nations in order to get an effective and unified response to climate change. Building a critical mass for action and an institutional memory to sustain policies and efforts is crucial. A resilient-based approach to climate mitigation and adaptation founded on a crisis-driven reaction to communities vulnerable to climate threat will boost quality of life through the provision and safeguarding of safety nets for the poor. Solar water heaters, a renewable energy source, are here considered as a critical option to South Africa’s coal-driven economy as a means of alleviating energy poverty in rural and low-income communities, to be more precise. Therefore, SWHs have become the epicentre of sustainable development policies and climate change mitigation efforts in South Africa. So far, this device has attracted the attention of local governments in the country who view it as a game changer in the field, particularly towards greenhouse gas emission and energy conservation. The contour of this thesis is to assess the impact of solar water heaters on sustainable development. Although it is generally difficult to assess the real impact of technology on people, that of SWHs was established by asking questions about livelihood before, during and after the introduction of this device.
- Full Text:
- Date Issued: 2014
- Authors: Mbeng, Regina Nso
- Date: 2014
- Subjects: Solar water heaters , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9238 , http://hdl.handle.net/10948/d1021103
- Description: In this present world order of growing information, communication and technological advancement, it is primordial that climate change adaptation and mitigation efforts seeks to make people resilient to inevitable climate inconsistency through the exploitation and development of renewable energy sources. Though climate impacts are global, the population most at risk is predominantly from developing countries, particularly poor communities who have experienced multi-layered threats from floods, droughts and energy supply, just to mention a few. This explains why sustainable development is at the heart of all development trajectories in the world today, specifically the post-2015 development tenets. Climate change adaptation and mitigation requires international collaboration from all nations in order to get an effective and unified response to climate change. Building a critical mass for action and an institutional memory to sustain policies and efforts is crucial. A resilient-based approach to climate mitigation and adaptation founded on a crisis-driven reaction to communities vulnerable to climate threat will boost quality of life through the provision and safeguarding of safety nets for the poor. Solar water heaters, a renewable energy source, are here considered as a critical option to South Africa’s coal-driven economy as a means of alleviating energy poverty in rural and low-income communities, to be more precise. Therefore, SWHs have become the epicentre of sustainable development policies and climate change mitigation efforts in South Africa. So far, this device has attracted the attention of local governments in the country who view it as a game changer in the field, particularly towards greenhouse gas emission and energy conservation. The contour of this thesis is to assess the impact of solar water heaters on sustainable development. Although it is generally difficult to assess the real impact of technology on people, that of SWHs was established by asking questions about livelihood before, during and after the introduction of this device.
- Full Text:
- Date Issued: 2014
The potential for solar water heaters in urban development in KwaZulu-Natal
- Authors: Dlepu, Thobisa Miranda
- Date: 2014
- Subjects: Solar water heaters , Solar heating , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9251 , http://hdl.handle.net/10948/d1021184
- Description: The study found that investing in solar water heater system (SWH) would be an economically, socially and environmentally viable option to implement within urban households; in which the hot water demand, volume of hot water consumption, water heating costs, and electricity tariffs are high. Investing in solar water heater technology should be the key focus of South African government; in order to enhance the wellbeing of the ordinary citizens. The study made use of the qualitative research approach to investigate solar water heater drivers and barriers; from the perspective of energy consumers. This will be achieved by focusing on the major drivers and barriers that consumers consider when making decisions on adoption of solar water heater system. It is believed that solar water heater drivers and barriers substantially influence the potential and urban development thereof. Solar heat is utilized in the residential sector for heating water for bathing, cleaning, washing and cooking food, but its availability is limited (Arora & Arora, 2013). Renewable energy has become a wild card internationally and regionally because of the technical, economic and environmental barriers affecting the disadvantaged individuals.
- Full Text:
- Date Issued: 2014
- Authors: Dlepu, Thobisa Miranda
- Date: 2014
- Subjects: Solar water heaters , Solar heating , Renewable energy sources
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9251 , http://hdl.handle.net/10948/d1021184
- Description: The study found that investing in solar water heater system (SWH) would be an economically, socially and environmentally viable option to implement within urban households; in which the hot water demand, volume of hot water consumption, water heating costs, and electricity tariffs are high. Investing in solar water heater technology should be the key focus of South African government; in order to enhance the wellbeing of the ordinary citizens. The study made use of the qualitative research approach to investigate solar water heater drivers and barriers; from the perspective of energy consumers. This will be achieved by focusing on the major drivers and barriers that consumers consider when making decisions on adoption of solar water heater system. It is believed that solar water heater drivers and barriers substantially influence the potential and urban development thereof. Solar heat is utilized in the residential sector for heating water for bathing, cleaning, washing and cooking food, but its availability is limited (Arora & Arora, 2013). Renewable energy has become a wild card internationally and regionally because of the technical, economic and environmental barriers affecting the disadvantaged individuals.
- Full Text:
- Date Issued: 2014
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