The intersection of culture and gender in constructions of ukuzila’ (spousal mourning) among AmaXhosa in the Eastern Cape:
- Ngqangweni, Hlonelwa, Macleod, Catriona I
- Authors: Ngqangweni, Hlonelwa , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143615 , vital:38267 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Mourning is a universal and culturally specific practice following the death of a significant other. The Xhosa equivalent of the mourning process is ukuzila. Very little has been written on the subject of ukuzila in spite of the detrimental effects of the practice on the widows’ health and safety, as well as the discriminatory nature of the practice. This paper presents the findings of a discourse analytic qualitative study conducted among isiXhosa speaking men and women in South Africa. The study revealed ukuzila as a practice put in place to show respect to the deceased. However, the showing of respect revealed a historically gendered cultural practice, imbued with power relations and centred on ‘visibility’. In light of this finding, the authors propose further research which includes exploring people’s willingness to change to a non-gendered practice of ukuzila, and alternate expressions of ukuzila that suit women rather than ‘culture’ and society.
- Full Text:
- Date Issued: 2015
- Authors: Ngqangweni, Hlonelwa , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143615 , vital:38267 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Mourning is a universal and culturally specific practice following the death of a significant other. The Xhosa equivalent of the mourning process is ukuzila. Very little has been written on the subject of ukuzila in spite of the detrimental effects of the practice on the widows’ health and safety, as well as the discriminatory nature of the practice. This paper presents the findings of a discourse analytic qualitative study conducted among isiXhosa speaking men and women in South Africa. The study revealed ukuzila as a practice put in place to show respect to the deceased. However, the showing of respect revealed a historically gendered cultural practice, imbued with power relations and centred on ‘visibility’. In light of this finding, the authors propose further research which includes exploring people’s willingness to change to a non-gendered practice of ukuzila, and alternate expressions of ukuzila that suit women rather than ‘culture’ and society.
- Full Text:
- Date Issued: 2015
All about the abs: discourse of health in the negotiation of masculine body-image
- Authors: Plüg, Simóne
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143793 , vital:38283 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: This paper explores contemporary South African masculinities and how aspects of consumer culture interweave the self and body-image where “the prime purpose of the maintenance of the inner body becomes the enhancement of the appearance of the outer body” (Featherstone, 1991, p. 171). It details a study of young men in Durban, using a qualitative research design and a social constructionist theoretical framework to explore the discourses participants use when discussing their own and other male bodies. It highlights the ways in which consumerism, the media, and other social dynamics promote and silence different discourses around what constitutes a desirable man in 21st century South Africa. The paper presents a detailed exploration of the ‘healthy body’ discourse, discussing how it shaped men’s engagement in self-sculpting practices and provided a means by which the participants came to understand and manage their gendered identities.
- Full Text:
- Date Issued: 2015
- Authors: Plüg, Simóne
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143793 , vital:38283 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: This paper explores contemporary South African masculinities and how aspects of consumer culture interweave the self and body-image where “the prime purpose of the maintenance of the inner body becomes the enhancement of the appearance of the outer body” (Featherstone, 1991, p. 171). It details a study of young men in Durban, using a qualitative research design and a social constructionist theoretical framework to explore the discourses participants use when discussing their own and other male bodies. It highlights the ways in which consumerism, the media, and other social dynamics promote and silence different discourses around what constitutes a desirable man in 21st century South Africa. The paper presents a detailed exploration of the ‘healthy body’ discourse, discussing how it shaped men’s engagement in self-sculpting practices and provided a means by which the participants came to understand and manage their gendered identities.
- Full Text:
- Date Issued: 2015
Unravelling the myths about unplanned pregnancy among female students at University of Fort Hare East London Campus, South Africa
- Authors: Chukwunyere , Amadi P
- Date: 2015
- Subjects: Unwanted pregnancy , Women college students , College students
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10353/25415 , vital:64237
- Description: This research study examined: 1. the attitudes and perceptions of female university students towards unplanned pregnancies, their (pregnancies’) associated influences and their social economic consequences on the participants and their family. 2. The level of knowledge, the sources thereof, and the girls’ attitudes and perceptions toward contraception, abortion and their actual use as possible barriers in the prevention of unplanned pregnancies. 3. The possible interventions in curbing the phenomenon of unplanned pregnancies. Data for the study was gathered through in-depth-interviews with ten female undergraduate students at the University of Fort Hare in East London campus, who had the experience of unplanned pregnancy while studying. Selection of participants was done through snowball sampling. A number of factors responsible for the unplanned pregnancy are revealed, including: Inadequate and inconsistent use of contraceptives, ignorance about contraception or rejection of contraceptive use due to religious beliefs. Influential factors regarding unplanned child bearing include: The role of the mother and the boyfriend, traditional and religious beliefs, and the desire for child bearing and motherhood. Although the majority of the girls are knowledgeable about contraception, and their right to abortion, a number of barriers hinder them from putting this knowledge into practical use. Some of the most highlighted consequences of unplanned/unwanted pregnancy are the financial burden on the family and loss of study time. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2015
- Full Text:
- Date Issued: 2015
- Authors: Chukwunyere , Amadi P
- Date: 2015
- Subjects: Unwanted pregnancy , Women college students , College students
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10353/25415 , vital:64237
- Description: This research study examined: 1. the attitudes and perceptions of female university students towards unplanned pregnancies, their (pregnancies’) associated influences and their social economic consequences on the participants and their family. 2. The level of knowledge, the sources thereof, and the girls’ attitudes and perceptions toward contraception, abortion and their actual use as possible barriers in the prevention of unplanned pregnancies. 3. The possible interventions in curbing the phenomenon of unplanned pregnancies. Data for the study was gathered through in-depth-interviews with ten female undergraduate students at the University of Fort Hare in East London campus, who had the experience of unplanned pregnancy while studying. Selection of participants was done through snowball sampling. A number of factors responsible for the unplanned pregnancy are revealed, including: Inadequate and inconsistent use of contraceptives, ignorance about contraception or rejection of contraceptive use due to religious beliefs. Influential factors regarding unplanned child bearing include: The role of the mother and the boyfriend, traditional and religious beliefs, and the desire for child bearing and motherhood. Although the majority of the girls are knowledgeable about contraception, and their right to abortion, a number of barriers hinder them from putting this knowledge into practical use. Some of the most highlighted consequences of unplanned/unwanted pregnancy are the financial burden on the family and loss of study time. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2015
- Full Text:
- Date Issued: 2015
Privilege, solidarity and social justice struggles in South Africa: a view from Grahamstown
- Authors: Matthews, Sally
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142270 , vital:38064 , DOI:10.1353/trn.2015.0016
- Description: The last decade has seen a significant increase in the number and prominence of social movements in South Africa. Many of these movements are supported by relatively privileged individuals who are not themselves victims of the injustices the movements oppose. In this paper, I draw out some of the possibilities and limitations of the role of privileged individuals in supporting social movements struggling for social justice, looking particularly at the role of students in supporting such movements. The paper is based on research on the relationship between one such movement, the Unemployed People’s Movement (UPM), and a student organisation, the Students for Social Justice (SSJ), both of which are based in Grahamstown, South Africa. While acknowledging that privileged supporters of such movements can play a constructive role in social justice struggles, I use the experiences of the UPM and SSJ to explore some of the tensions that are likely to emerge and that need to be addressed when the relatively privileged participate in popular struggles. In particular, I discuss the likely difficulties privileged supporters will experience in bridging social divides and in contributing meaningfully to the theorisation of popular struggles.
- Full Text:
- Date Issued: 2015
- Authors: Matthews, Sally
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142270 , vital:38064 , DOI:10.1353/trn.2015.0016
- Description: The last decade has seen a significant increase in the number and prominence of social movements in South Africa. Many of these movements are supported by relatively privileged individuals who are not themselves victims of the injustices the movements oppose. In this paper, I draw out some of the possibilities and limitations of the role of privileged individuals in supporting social movements struggling for social justice, looking particularly at the role of students in supporting such movements. The paper is based on research on the relationship between one such movement, the Unemployed People’s Movement (UPM), and a student organisation, the Students for Social Justice (SSJ), both of which are based in Grahamstown, South Africa. While acknowledging that privileged supporters of such movements can play a constructive role in social justice struggles, I use the experiences of the UPM and SSJ to explore some of the tensions that are likely to emerge and that need to be addressed when the relatively privileged participate in popular struggles. In particular, I discuss the likely difficulties privileged supporters will experience in bridging social divides and in contributing meaningfully to the theorisation of popular struggles.
- Full Text:
- Date Issued: 2015
Visible Wars and Invisible Women: interrogating women's roles during wartime in Goretti Kyomuhendo's Waiting: a novel of Uganda at war
- Authors: Spencer, Lynda G
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/138880 , vital:37682 , https://0-hdl.handle.net.wam.seals.ac.za/10520/EJC177790
- Description: Goretti Kyomuhendo's Waiting: A Novel of Uganda at War explores the atrocities that ordinary people experience during wartime by placing emphasis on the private suffering and humiliation inflicted on women in the domestic space of the home. This article argues that even if women do not actively feature on the battleground, they are still inadvertently drawn into the war, which has an adverse impact on their lives. Kyomuhendo draws on the experiences of different female characters to problematize the inherently ambiguous symbolic image of the mother, and shows that the violence performed on women's bodies is a result of the interplay between two hegemonic forces, patriarchal authority and state power.
- Full Text:
- Date Issued: 2015
- Authors: Spencer, Lynda G
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/138880 , vital:37682 , https://0-hdl.handle.net.wam.seals.ac.za/10520/EJC177790
- Description: Goretti Kyomuhendo's Waiting: A Novel of Uganda at War explores the atrocities that ordinary people experience during wartime by placing emphasis on the private suffering and humiliation inflicted on women in the domestic space of the home. This article argues that even if women do not actively feature on the battleground, they are still inadvertently drawn into the war, which has an adverse impact on their lives. Kyomuhendo draws on the experiences of different female characters to problematize the inherently ambiguous symbolic image of the mother, and shows that the violence performed on women's bodies is a result of the interplay between two hegemonic forces, patriarchal authority and state power.
- Full Text:
- Date Issued: 2015
How general-purpose can a GPU be?
- Authors: Machanick, Philip
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61180 , vital:27988 , http://dx.doi.org/10.18489/sacj.v0i57.347
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple datastream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions. Supercomputers covered a range of exotic designs such as hypercubes and the Connection Machine (Fox, 1989). The latter is likely the source of the snide comment by Cray: it had thousands of relatively low-speed CPUs (Tucker & Robertson, 1988). Since Cray won, why are we not basing our ideas on his designs (Cray Inc., 2004), rather than those of the losers? The Top 500 supercomputer list is dominated by general-purpose CPUs, and nothing like the Connection Machine that headed the list in 1993 still exists.
- Full Text:
- Date Issued: 2015
- Authors: Machanick, Philip
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61180 , vital:27988 , http://dx.doi.org/10.18489/sacj.v0i57.347
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple datastream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions. Supercomputers covered a range of exotic designs such as hypercubes and the Connection Machine (Fox, 1989). The latter is likely the source of the snide comment by Cray: it had thousands of relatively low-speed CPUs (Tucker & Robertson, 1988). Since Cray won, why are we not basing our ideas on his designs (Cray Inc., 2004), rather than those of the losers? The Top 500 supercomputer list is dominated by general-purpose CPUs, and nothing like the Connection Machine that headed the list in 1993 still exists.
- Full Text:
- Date Issued: 2015
Subjective well-being in Africa
- Botha, Ferdi, Snowball, Jeanette D
- Authors: Botha, Ferdi , Snowball, Jeanette D
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61084 , vital:27946
- Description: Research on quality of life and subjective well-being (SWB) has witnessed a remarkable growth over the past four decades or so. Since Easterlin’s (1974) seminal contribution on the relationship between happiness and income, thousands of studies have followed that examine the intricacies of subjective well-being (for reviews, see Frey and Stutzer, 2002; Dolan et al., 2008; MacKerron, 2012). These studies have uncovered some very important aspects of individual well-being and have pointed to the fact that money or income is not always (as is often assumed) the most important determinant of SWB.
- Full Text:
- Date Issued: 2015
- Authors: Botha, Ferdi , Snowball, Jeanette D
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61084 , vital:27946
- Description: Research on quality of life and subjective well-being (SWB) has witnessed a remarkable growth over the past four decades or so. Since Easterlin’s (1974) seminal contribution on the relationship between happiness and income, thousands of studies have followed that examine the intricacies of subjective well-being (for reviews, see Frey and Stutzer, 2002; Dolan et al., 2008; MacKerron, 2012). These studies have uncovered some very important aspects of individual well-being and have pointed to the fact that money or income is not always (as is often assumed) the most important determinant of SWB.
- Full Text:
- Date Issued: 2015
Addressing local level food insecurity amongst small-holder communities in transition
- Shackleton, Charlie M, Hamer, Nicholas G, Swallow, Brent M, Ncube, K
- Authors: Shackleton, Charlie M , Hamer, Nicholas G , Swallow, Brent M , Ncube, K
- Date: 2015
- Subjects: Food security -- South Africa Economic development -- South Africa Rural development -- South Africa
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/50090 , vital:25958
- Description: Food insecurity affects as significant proportion of the world's population and hence it typically receives priority attention in global policies associated with poverty, equity and sustainable development. For example, it is the first of the Millennium Development Goals and the second of their successor, the Sustainable Development Goals. Access to sufficient and nutritious food is deemed a basic human right. The latest FAO analysis of the “State of Food Insecurity in the World 2014” reports that 805 million people (approximately 11-12% of the world's population) are chronically undernourished (i.e. do not have sufficient energy intake over a period of at least one year). In sub-Saharan Africa the prevalence remains stubbornly high at 24%, the highest in the world. Whilst most interpret food insecurity to mean an insufficient quantity of food (as measured by the number of calories consumed), the widely accepted FAO definition considers four dimensions of food security, namely quantity, quality or diversity, access and use. Provision of enough calories on a daily basis is not sufficient if the diet lacks diversity and appropriate balance to provide the full range of minerals and vitamins necessary for proper health, or if the food available is culturally unacceptable. Thus, there is a pressing need for more nuanced analyses of food security against all four of the dimensions embedded in the concept. Additionally, it is important that these be measured at more local or regional levels because national statistics can mask alarming regional discrepancies in food security, or amongst particular sectors of society, such as recent migrants, refugees, female- or child-headed households, those vulnerable to HIV/AIDS or the landless, to mention just a few. For example, at a national level South Africa is considered a food secure nation with respect to staple requirements, and access to sufficient food is enshrined in the Constitution (Section 27, subsection 1b), but nationally one in twenty (i.e. approx. 2.5 million people) go to bed hungry most nights, and 23% of children below the age of 15 are physically stunted, severely stunted or wasted, due to the long-term ill effects of insufficient food or of inadequate diversity and quality. At a subnational level, there are marked differences between rural and urban populations and even between geographic areas (for example, the prevalence of stunting amongst boys less than 15 years old is 23% in the Eastern Cape, compared to 12% in Gauteng). Once again, despite being a food secure nation, nationally 40% of the population have a dietary diversity score of four or less, which is a cut-off point signifying poor dietary diversity which makes people more vulnerable to malnutrition and ill health, and in Limpopo and Northwest provinces it is as high as 66% and 61%, respectively.
- Full Text:
- Date Issued: 2015
- Authors: Shackleton, Charlie M , Hamer, Nicholas G , Swallow, Brent M , Ncube, K
- Date: 2015
- Subjects: Food security -- South Africa Economic development -- South Africa Rural development -- South Africa
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/50090 , vital:25958
- Description: Food insecurity affects as significant proportion of the world's population and hence it typically receives priority attention in global policies associated with poverty, equity and sustainable development. For example, it is the first of the Millennium Development Goals and the second of their successor, the Sustainable Development Goals. Access to sufficient and nutritious food is deemed a basic human right. The latest FAO analysis of the “State of Food Insecurity in the World 2014” reports that 805 million people (approximately 11-12% of the world's population) are chronically undernourished (i.e. do not have sufficient energy intake over a period of at least one year). In sub-Saharan Africa the prevalence remains stubbornly high at 24%, the highest in the world. Whilst most interpret food insecurity to mean an insufficient quantity of food (as measured by the number of calories consumed), the widely accepted FAO definition considers four dimensions of food security, namely quantity, quality or diversity, access and use. Provision of enough calories on a daily basis is not sufficient if the diet lacks diversity and appropriate balance to provide the full range of minerals and vitamins necessary for proper health, or if the food available is culturally unacceptable. Thus, there is a pressing need for more nuanced analyses of food security against all four of the dimensions embedded in the concept. Additionally, it is important that these be measured at more local or regional levels because national statistics can mask alarming regional discrepancies in food security, or amongst particular sectors of society, such as recent migrants, refugees, female- or child-headed households, those vulnerable to HIV/AIDS or the landless, to mention just a few. For example, at a national level South Africa is considered a food secure nation with respect to staple requirements, and access to sufficient food is enshrined in the Constitution (Section 27, subsection 1b), but nationally one in twenty (i.e. approx. 2.5 million people) go to bed hungry most nights, and 23% of children below the age of 15 are physically stunted, severely stunted or wasted, due to the long-term ill effects of insufficient food or of inadequate diversity and quality. At a subnational level, there are marked differences between rural and urban populations and even between geographic areas (for example, the prevalence of stunting amongst boys less than 15 years old is 23% in the Eastern Cape, compared to 12% in Gauteng). Once again, despite being a food secure nation, nationally 40% of the population have a dietary diversity score of four or less, which is a cut-off point signifying poor dietary diversity which makes people more vulnerable to malnutrition and ill health, and in Limpopo and Northwest provinces it is as high as 66% and 61%, respectively.
- Full Text:
- Date Issued: 2015
Physicochemical behavior of nanohybrids of mono and tetra substituted carboxyphenoxy phthalocyanine covalently linked to GSH–CdTe/CdS/ZnS quantum dots
- Oluwole, David O, Nyokong, Tebello
- Authors: Oluwole, David O , Nyokong, Tebello
- Date: 2015
- Language: English
- Type: text , Article
- Identifier: vital:7266 , http://hdl.handle.net/10962/d1020275 , http://dx.doi.org/10.1016/j.poly.2014.10.024
- Description: Zinc monocarboxyphenoxy and tetracarboxyphenoxy phthalocyanines were covalently linked with three different sizes of glutathione capped core/shell/shell {CdTe/CdS/ZnS(4.2), CdTe/CdS/ZnS(5.1) and CdTe/CdS/ZnS(6.7)}; a core shell {CdTe/CdS(3.1)} and core {CdTe(2.4)} quantum dots. The physicochemical behavior and Förster resonance energy transfer (FRET) processes of the nanohybrids were investigated. The highest FRET efficiency was observed with CdTe/CdS/ZnS(6.7) nanohybrids with 98% and the least efficiency was observed with CdTe(2.4) nanohybrids with 85%. The CdTe/CdS/ZnS(6.7) also showed the best physicochemical behavior. These good physicochemical properties make the synthesized nanohybrids viable photosensitizers. , Original publication is available at http://dx.doi.org/10.1016/j.poly.2014.10.024
- Full Text: false
- Date Issued: 2015
- Authors: Oluwole, David O , Nyokong, Tebello
- Date: 2015
- Language: English
- Type: text , Article
- Identifier: vital:7266 , http://hdl.handle.net/10962/d1020275 , http://dx.doi.org/10.1016/j.poly.2014.10.024
- Description: Zinc monocarboxyphenoxy and tetracarboxyphenoxy phthalocyanines were covalently linked with three different sizes of glutathione capped core/shell/shell {CdTe/CdS/ZnS(4.2), CdTe/CdS/ZnS(5.1) and CdTe/CdS/ZnS(6.7)}; a core shell {CdTe/CdS(3.1)} and core {CdTe(2.4)} quantum dots. The physicochemical behavior and Förster resonance energy transfer (FRET) processes of the nanohybrids were investigated. The highest FRET efficiency was observed with CdTe/CdS/ZnS(6.7) nanohybrids with 98% and the least efficiency was observed with CdTe(2.4) nanohybrids with 85%. The CdTe/CdS/ZnS(6.7) also showed the best physicochemical behavior. These good physicochemical properties make the synthesized nanohybrids viable photosensitizers. , Original publication is available at http://dx.doi.org/10.1016/j.poly.2014.10.024
- Full Text: false
- Date Issued: 2015
Visible light transformation of Rhodamine 6G using tetracarbazole zinc phthalocyanine when embedded in electrospun fibers and in the presence of ZnO and Ag particles
- Khoza, Phindile, Nyokong, Tebello
- Authors: Khoza, Phindile , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189456 , vital:44848 , xlink:href="https://doi.org/10.1080/00958972.2015.1013944"
- Description: Herein, we report the photocatalytic transformation of Rhodamine 6G (Rh 6G) using tetracarbazole zinc phthalocyanine (TCbZnPc) when alone or when conjugated with ZnO macroparticles (ZnOMPs) and silver nanoparticles (AgNPs), represented as TCbZnPc–ZnOMPs and TCbZnPc–AgNPs, respectively. The photocatalysts were supported onto electrospun polystyrene fibers. The efficiency of TCbZnPc was improved by the presence of both ZnOMPs and AgNPs. HPLC equipped with UV–vis was used to study phototransformation products. The mechanism of transformation was via the N-de-ethylation of Rh 6G.
- Full Text:
- Date Issued: 2015
- Authors: Khoza, Phindile , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189456 , vital:44848 , xlink:href="https://doi.org/10.1080/00958972.2015.1013944"
- Description: Herein, we report the photocatalytic transformation of Rhodamine 6G (Rh 6G) using tetracarbazole zinc phthalocyanine (TCbZnPc) when alone or when conjugated with ZnO macroparticles (ZnOMPs) and silver nanoparticles (AgNPs), represented as TCbZnPc–ZnOMPs and TCbZnPc–AgNPs, respectively. The photocatalysts were supported onto electrospun polystyrene fibers. The efficiency of TCbZnPc was improved by the presence of both ZnOMPs and AgNPs. HPLC equipped with UV–vis was used to study phototransformation products. The mechanism of transformation was via the N-de-ethylation of Rh 6G.
- Full Text:
- Date Issued: 2015
Global Handbook of Quality of Life: exploration of well-being of nations and continents
- Glatzer, Wolfgang, Camfield, Laura, Moller, Valerie, Rojas, Mariano
- Authors: Glatzer, Wolfgang , Camfield, Laura , Moller, Valerie , Rojas, Mariano
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67245 , vital:29063 , https://doi.org/10.1007/978-94-017-9178-6
- Description: publisher version , This handbook provides a comprehensive historical account of the field of Quality of Life. It brings together theoretical insights and empirical findings, and presents the main items of global quality of life and wellbeing research. Worldwide in its scope of topics, the handbook examines discussions of demographic and health development, the spread of democracy, global economic accounting, multi-item measurement of perceived satisfaction and expert-assessed quality of life, and the well-being of children, women and poor people. It looks at well-being in specific regions, including North and Sub-Saharan Africa, Asia, South America, and Eastern and Western Europe. In addition to contributions by leading and younger authors, the handbook includes contributions from International Organizations about their own work with respect to social reporting.
- Full Text: false
- Date Issued: 2015
- Authors: Glatzer, Wolfgang , Camfield, Laura , Moller, Valerie , Rojas, Mariano
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67245 , vital:29063 , https://doi.org/10.1007/978-94-017-9178-6
- Description: publisher version , This handbook provides a comprehensive historical account of the field of Quality of Life. It brings together theoretical insights and empirical findings, and presents the main items of global quality of life and wellbeing research. Worldwide in its scope of topics, the handbook examines discussions of demographic and health development, the spread of democracy, global economic accounting, multi-item measurement of perceived satisfaction and expert-assessed quality of life, and the well-being of children, women and poor people. It looks at well-being in specific regions, including North and Sub-Saharan Africa, Asia, South America, and Eastern and Western Europe. In addition to contributions by leading and younger authors, the handbook includes contributions from International Organizations about their own work with respect to social reporting.
- Full Text: false
- Date Issued: 2015
Scientific frontiers in the management of coral reefs
- Aswani, Shankar, Mumby, Peter J, Baker, Andrew, Christie, Patrick, McCook, Laurence J, Steneck, Robert S, Richmond, Robert H
- Authors: Aswani, Shankar , Mumby, Peter J , Baker, Andrew , Christie, Patrick , McCook, Laurence J , Steneck, Robert S , Richmond, Robert H
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/70582 , vital:29677 , https://doi.org/10.3389/fmars.2015.00050
- Description: Coral reefs are subjected globally to a variety of natural and anthropogenic stressors that often act synergistically. Today, reversing ongoing and future coral reef degradation presents significant challenges and countering this negative trend will take considerable efforts and investments. Scientific knowledge can inform and guide the requisite decision-making process and offer practical solutions to the problem of protection as the effects of climate change exacerbate. However, implementation of solutions presently lags far behind the pace required to reverse global declines, and there is a need for an urgent and significant step-up in the extent and range of strategies being implemented. In this paper, we consider scientific frontiers in natural and social science research that can help build stronger support for reef management and improve the efficacy of interventions. We cover various areas including: (1) enhancing the case for reef conservation and management, (2) dealing with local stressors on reefs, (3) addressing global climate change impacts, (4) and reviewing various approaches to the governance of coral reefs. In sum, we consider scientific frontiers in natural and social science that will require further attention in coming years as managers work toward building stronger support for reef management and improve the efficacy of local interventions.
- Full Text:
- Date Issued: 2015
- Authors: Aswani, Shankar , Mumby, Peter J , Baker, Andrew , Christie, Patrick , McCook, Laurence J , Steneck, Robert S , Richmond, Robert H
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/70582 , vital:29677 , https://doi.org/10.3389/fmars.2015.00050
- Description: Coral reefs are subjected globally to a variety of natural and anthropogenic stressors that often act synergistically. Today, reversing ongoing and future coral reef degradation presents significant challenges and countering this negative trend will take considerable efforts and investments. Scientific knowledge can inform and guide the requisite decision-making process and offer practical solutions to the problem of protection as the effects of climate change exacerbate. However, implementation of solutions presently lags far behind the pace required to reverse global declines, and there is a need for an urgent and significant step-up in the extent and range of strategies being implemented. In this paper, we consider scientific frontiers in natural and social science research that can help build stronger support for reef management and improve the efficacy of interventions. We cover various areas including: (1) enhancing the case for reef conservation and management, (2) dealing with local stressors on reefs, (3) addressing global climate change impacts, (4) and reviewing various approaches to the governance of coral reefs. In sum, we consider scientific frontiers in natural and social science that will require further attention in coming years as managers work toward building stronger support for reef management and improve the efficacy of local interventions.
- Full Text:
- Date Issued: 2015
Women’s micro-narratives of the process of abortion decision-making: justifying the decision to have an abortion
- Mavuso, Jabulile, Macleod, Catriona I
- Authors: Mavuso, Jabulile , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143893 , vital:38292 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: What is missing from abortion research is research that explores women’s narratives of the processes of abortion decision-making in a way that acknowledges the constraints placed on ‘choice’. This study sought to explore, using Foucauldian feminist post-structuralism and a narrative-discursive approach, women’s micro-narratives of the abortion decision-making process. Purposive sampling was used to recruit a total of 25 participants from three abortion facilities in the Eastern Cape. Participants were unmarried ‘Black’ women between the ages of 19 and 35, and were mostly unemployed. Narrative interviews were done with the women. Analysis revealed an over-arching narrative in which women described the abortion decision as something that they were ‘forced’ into by their circumstances. To construct this narrative, women justified the decision to have an abortion by drawing on discourses that normalise certain practices located within the husband-wife and parent-child axes and make the pregnancy a problematic, unsupported and unsupportable one. Gendered and generational power relations reinforced this and contributed to the obstruction of reproductive justice.
- Full Text:
- Date Issued: 2015
- Authors: Mavuso, Jabulile , Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143893 , vital:38292 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: What is missing from abortion research is research that explores women’s narratives of the processes of abortion decision-making in a way that acknowledges the constraints placed on ‘choice’. This study sought to explore, using Foucauldian feminist post-structuralism and a narrative-discursive approach, women’s micro-narratives of the abortion decision-making process. Purposive sampling was used to recruit a total of 25 participants from three abortion facilities in the Eastern Cape. Participants were unmarried ‘Black’ women between the ages of 19 and 35, and were mostly unemployed. Narrative interviews were done with the women. Analysis revealed an over-arching narrative in which women described the abortion decision as something that they were ‘forced’ into by their circumstances. To construct this narrative, women justified the decision to have an abortion by drawing on discourses that normalise certain practices located within the husband-wife and parent-child axes and make the pregnancy a problematic, unsupported and unsupportable one. Gendered and generational power relations reinforced this and contributed to the obstruction of reproductive justice.
- Full Text:
- Date Issued: 2015
The law of divorce and dissolution of life partnerships in South Africa: book review
- Authors: Kruuse, Helen
- Date: 2015
- Language: English
- Type: Article , text
- Identifier: http://hdl.handle.net/10962/54129 , vital:26394 , http://scholar.ufs.ac.za:8080/xmlui/handle/11660/2454
- Description: Jackie Heaton’s latest contribution to the family law domain is formidable – 777 pages of carefully crafted opinions and discussions of the law affecting divorce and dissolution of life partnerships. Given the range, diversity and depth of issues in this area, it is no wonder that she calls on those being among the best in their field to assist her in writing up the book.
- Full Text:
- Date Issued: 2015
- Authors: Kruuse, Helen
- Date: 2015
- Language: English
- Type: Article , text
- Identifier: http://hdl.handle.net/10962/54129 , vital:26394 , http://scholar.ufs.ac.za:8080/xmlui/handle/11660/2454
- Description: Jackie Heaton’s latest contribution to the family law domain is formidable – 777 pages of carefully crafted opinions and discussions of the law affecting divorce and dissolution of life partnerships. Given the range, diversity and depth of issues in this area, it is no wonder that she calls on those being among the best in their field to assist her in writing up the book.
- Full Text:
- Date Issued: 2015
An assessment of chlorophyll-a concentration spatio-temporal variation using Landsat satellite data, in a small tropical reservoir
- Dalu, Tatenda, Dube, Timothy, Froneman, P William, Sachikonye, Mwazvita T B, Clegg, Bruce W, Nhiwatiwa, Tamuka
- Authors: Dalu, Tatenda , Dube, Timothy , Froneman, P William , Sachikonye, Mwazvita T B , Clegg, Bruce W , Nhiwatiwa, Tamuka
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68042 , vital:29189 , https://doi.org/10.1080/10106049.2015.1027292
- Description: Publisher version , Traditional approaches to monitoring aquatic systems are often limited by the need for data collection which often is time-consuming, expensive and non-continuous. The aim of the study was to map the spatio-temporal chlorophyll-a concentration changes in Malilangwe Reservoir, Zimbabwe as an indicator of phytoplankton biomass and trophic state when the reservoir was full (year 2000) and at its lowest capacity (year 2011), using readily available Landsat multispectral images. Medium-spatial resolution (30 m) Landsat multispectral Thematic Mapper TM 5 and ETM+ images for May to December 1999–2000 and 2010–2011 were used to derive chlorophyll-a concentrations. In situ measured chlorophyll-a and total suspended solids (TSS) concentrations for 2011 were employed to validate the Landsat chlorophyll-a and TSS estimates. The study results indicate that Landsat-derived chlorophyll-a and TSS estimates were comparable with field measurements. There was a considerable wet vs. dry season differences in total chlorophyll-a concentration, Secchi disc depth, TSS and turbidity within the reservoir. Using Permutational multivariate analyses of variance (PERMANOVA) analysis, there were significant differences (p < 0.0001) for chlorophyll-a concentration among sites, months and years whereas TSS was significant during the study months (p < 0.05). A strong positive significant correlation among both predicted TSS vs. chlorophyll-a and measured vs. predicted chlorophyll-a and TSS concentrations as well as an inverse relationship between reservoir chlorophyll-a concentrations and water level were found (p < 0.001 in all cases). In conclusion, total chlorophyll-a concentration in Malilangwe Reservoir was successfully derived from Landsat remote sensing data suggesting that the Landsat sensor is suitable for real-time monitoring over relatively short timescales and for small reservoirs. Satellite data can allow for surveying of chlorophyll-a concentration in aquatic ecosystems, thus, providing invaluable data in data scarce (limited on site ground measurements) environments.
- Full Text: false
- Date Issued: 2015
- Authors: Dalu, Tatenda , Dube, Timothy , Froneman, P William , Sachikonye, Mwazvita T B , Clegg, Bruce W , Nhiwatiwa, Tamuka
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68042 , vital:29189 , https://doi.org/10.1080/10106049.2015.1027292
- Description: Publisher version , Traditional approaches to monitoring aquatic systems are often limited by the need for data collection which often is time-consuming, expensive and non-continuous. The aim of the study was to map the spatio-temporal chlorophyll-a concentration changes in Malilangwe Reservoir, Zimbabwe as an indicator of phytoplankton biomass and trophic state when the reservoir was full (year 2000) and at its lowest capacity (year 2011), using readily available Landsat multispectral images. Medium-spatial resolution (30 m) Landsat multispectral Thematic Mapper TM 5 and ETM+ images for May to December 1999–2000 and 2010–2011 were used to derive chlorophyll-a concentrations. In situ measured chlorophyll-a and total suspended solids (TSS) concentrations for 2011 were employed to validate the Landsat chlorophyll-a and TSS estimates. The study results indicate that Landsat-derived chlorophyll-a and TSS estimates were comparable with field measurements. There was a considerable wet vs. dry season differences in total chlorophyll-a concentration, Secchi disc depth, TSS and turbidity within the reservoir. Using Permutational multivariate analyses of variance (PERMANOVA) analysis, there were significant differences (p < 0.0001) for chlorophyll-a concentration among sites, months and years whereas TSS was significant during the study months (p < 0.05). A strong positive significant correlation among both predicted TSS vs. chlorophyll-a and measured vs. predicted chlorophyll-a and TSS concentrations as well as an inverse relationship between reservoir chlorophyll-a concentrations and water level were found (p < 0.001 in all cases). In conclusion, total chlorophyll-a concentration in Malilangwe Reservoir was successfully derived from Landsat remote sensing data suggesting that the Landsat sensor is suitable for real-time monitoring over relatively short timescales and for small reservoirs. Satellite data can allow for surveying of chlorophyll-a concentration in aquatic ecosystems, thus, providing invaluable data in data scarce (limited on site ground measurements) environments.
- Full Text: false
- Date Issued: 2015
Specific rewards for tax compliance: responses of small business owners in Ekurhuleni, South Africa
- Bornman, Marina, Stack, Elizabeth M
- Authors: Bornman, Marina , Stack, Elizabeth M
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/145325 , vital:38428 , https://heinonline.org/HOL/Page?handle=hein.journals/ejotaxrs13anddiv=35andg_sent=1andcasa_token=andcollection=journals
- Description: The literature reviewed documents the positive effects of rewards in encouraging desired behaviour, but rewards may have a crowding-in effect, strengthening intrinsic motivation, or a crowding-out effect, weakening it. External interventions may therefore be perceived as supportive, fostering self-esteem and self-determination, while those perceived as controlling may have the opposite effect. A number of countries have adopted a strategy of rewarding tax compliance. The rewards range from certificates awarded to compliant taxpayers, to privilege cards providing opportunities for discounts or special treatment, to lotteries in which compliant taxpayers can participate. The reward strategies are often accompanied by publicity programmes. Two such hypothetical strategies were presented to participants in a survey conducted amongst small business owners in Ekurhuleni, South Africa, to gauge their responses.
- Full Text:
- Date Issued: 2015
- Authors: Bornman, Marina , Stack, Elizabeth M
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/145325 , vital:38428 , https://heinonline.org/HOL/Page?handle=hein.journals/ejotaxrs13anddiv=35andg_sent=1andcasa_token=andcollection=journals
- Description: The literature reviewed documents the positive effects of rewards in encouraging desired behaviour, but rewards may have a crowding-in effect, strengthening intrinsic motivation, or a crowding-out effect, weakening it. External interventions may therefore be perceived as supportive, fostering self-esteem and self-determination, while those perceived as controlling may have the opposite effect. A number of countries have adopted a strategy of rewarding tax compliance. The rewards range from certificates awarded to compliant taxpayers, to privilege cards providing opportunities for discounts or special treatment, to lotteries in which compliant taxpayers can participate. The reward strategies are often accompanied by publicity programmes. Two such hypothetical strategies were presented to participants in a survey conducted amongst small business owners in Ekurhuleni, South Africa, to gauge their responses.
- Full Text:
- Date Issued: 2015
Educators and learners perceptions on english first additional language speakers use of english as medium of instruction
- Authors: Nqoma, Lungiswa
- Date: 2015
- Subjects: English language -- Study and teaching , English language
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24934 , vital:63678
- Description: This study investigates Educators‟ and learners‟ perceptions on English first additional language speakers‟ use of English as medium of instruction in two primary schools in the East London District. The study made use of a qualitative research method which includes interviews, observation and document analysis (learners‟ books). The uncovered data is analyzed against the background of the socio-economic and historical factors that led to this medium of instruction using content analysis (CA). After the apartheid regime in 1994 and the formation of a new government in 1996, the South African Constitution declared 11 languages in an attempt to encourage multilingualism in the country with each province having its provincial language policy. In the Eastern Cape where the study is conducted the language policy recognizes Xhosa, English, and Afrikaans as the official languages with English as the medium of instruction especially at the intermediate phase. The theoretical and conceptual framework for the study is provided by studies on language policy and planning, English as an international language, and Language as a mediating tool. The findings of the study indicate the crucial role that language of instruction plays in teaching and learning. However, challenges arise when the language of instruction is not the mother tongue. The study indicates dissatisfaction in the use of EMOI as a result of inadequate proficiency in the language of instruction, school environment, lack of resources; and lack of parental support. As a result of the above, some educators resorted to code switching when teaching. Hence, the study sheds light on EFAL learner‟s perspective on both comprehension and the level of educator‟s English skills and whether there is a connection between these two aspects. This resulted to conceivable methods that could be used to improve learner performance, teaching and learning methods and the efficiency of East London District support system. , Thesis (MEd) -- Faculty of Education, 2015
- Full Text:
- Date Issued: 2015
- Authors: Nqoma, Lungiswa
- Date: 2015
- Subjects: English language -- Study and teaching , English language
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24934 , vital:63678
- Description: This study investigates Educators‟ and learners‟ perceptions on English first additional language speakers‟ use of English as medium of instruction in two primary schools in the East London District. The study made use of a qualitative research method which includes interviews, observation and document analysis (learners‟ books). The uncovered data is analyzed against the background of the socio-economic and historical factors that led to this medium of instruction using content analysis (CA). After the apartheid regime in 1994 and the formation of a new government in 1996, the South African Constitution declared 11 languages in an attempt to encourage multilingualism in the country with each province having its provincial language policy. In the Eastern Cape where the study is conducted the language policy recognizes Xhosa, English, and Afrikaans as the official languages with English as the medium of instruction especially at the intermediate phase. The theoretical and conceptual framework for the study is provided by studies on language policy and planning, English as an international language, and Language as a mediating tool. The findings of the study indicate the crucial role that language of instruction plays in teaching and learning. However, challenges arise when the language of instruction is not the mother tongue. The study indicates dissatisfaction in the use of EMOI as a result of inadequate proficiency in the language of instruction, school environment, lack of resources; and lack of parental support. As a result of the above, some educators resorted to code switching when teaching. Hence, the study sheds light on EFAL learner‟s perspective on both comprehension and the level of educator‟s English skills and whether there is a connection between these two aspects. This resulted to conceivable methods that could be used to improve learner performance, teaching and learning methods and the efficiency of East London District support system. , Thesis (MEd) -- Faculty of Education, 2015
- Full Text:
- Date Issued: 2015
Optical properties of water-soluble L-cysteine-capped alloyed CdSeS quantum dot passivated with ZnSeTe and ZnSeTe/ZnS shells
- Adegoke, Oluwasesan, Nyokong, Tebello, Forbes, Patricia B C
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello , Forbes, Patricia B C
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193609 , vital:45352 , xlink:href="https://doi.org/10.1016/j.optmat.2015.05.024"
- Description: Alloyed quantum dots (QDs) passivated with shell materials have valuable optical characteristics suitable for a wide array of applications. In this work, alloyed ternary CdSeS QDs passivated with ZnSeTe and ZnSeTe/ZnS shells have been synthesized via a hot-injection method and a ligand exchange reaction employing L-cysteine as a thiol ligand has been used to obtain these water-soluble nanocrystals for the first time. The photoluminescence (PL) quantum yield (QY) of alloyed L-cysteine-capped CdSeS was 71.2% but decreased significantly to 5.2% upon passivation with a ZnSeTe shell. The red shift in PL emission of the CdSeS/ZnSeTe QDs was attributed to be strain-induced whilst a lattice-induced process likely created defect states in the core/shell interface hence contributing to the decline in the PL QY. Nonetheless, the fluorescence stability of CdSeS/ZnSeTe QDs in aqueous solution was unperturbed. Further passivation with a ZnS shell (CdSeS/ZnSeTe/ZnS) improved the PL QY to a value of 58.7% and thus indicates that the defect state in the QDs core/shell/shell structure was reduced. PL lifetime exciton measurements indicated that the rates of decay of the QDs influenced their photophysical properties.
- Full Text:
- Date Issued: 2015
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello , Forbes, Patricia B C
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193609 , vital:45352 , xlink:href="https://doi.org/10.1016/j.optmat.2015.05.024"
- Description: Alloyed quantum dots (QDs) passivated with shell materials have valuable optical characteristics suitable for a wide array of applications. In this work, alloyed ternary CdSeS QDs passivated with ZnSeTe and ZnSeTe/ZnS shells have been synthesized via a hot-injection method and a ligand exchange reaction employing L-cysteine as a thiol ligand has been used to obtain these water-soluble nanocrystals for the first time. The photoluminescence (PL) quantum yield (QY) of alloyed L-cysteine-capped CdSeS was 71.2% but decreased significantly to 5.2% upon passivation with a ZnSeTe shell. The red shift in PL emission of the CdSeS/ZnSeTe QDs was attributed to be strain-induced whilst a lattice-induced process likely created defect states in the core/shell interface hence contributing to the decline in the PL QY. Nonetheless, the fluorescence stability of CdSeS/ZnSeTe QDs in aqueous solution was unperturbed. Further passivation with a ZnS shell (CdSeS/ZnSeTe/ZnS) improved the PL QY to a value of 58.7% and thus indicates that the defect state in the QDs core/shell/shell structure was reduced. PL lifetime exciton measurements indicated that the rates of decay of the QDs influenced their photophysical properties.
- Full Text:
- Date Issued: 2015
Characterization of the co-chaperones of Hsp70 and Hsp90 in Trypanosoma brucei and their potential partnerships
- Authors: Mokoena, Fortunate
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54543 , vital:26583
- Description: African Trypanosomiasis, which is caused by Trypanosoma brucei, is one of the crippling agents of social and economic development in Africa. T. brucei cycles between the cold-blooded insect vector, the tsetse fly (Glossina spp), and warm-blooded mammalian hosts. T. brucei, T. cruzi and L. major are mammal infecting kinetoplastid parasites that are collectively referred to as TriTryps. These parasites experience extreme environments as they move between their warm-blooded mammalian hosts and cold-blooded insect vectors which trigger extensive morphological transformations during the life-cycle of the parasite. Molecular chaperones have been implicated in parasite differentiation. TriTryps display significant expansions and diversity in the gene complements encoding molecular chaperones, especially J-proteins. Generally, J-proteins function as co-chaperones of Hsp70s, forming part of vital protein homeostasis processes. Hsp70s show a high degree of conservation, while J-proteins appear to be an extreme case of taxonomic radiation. Although several studies have focused on the molecular and cell biology of Hsp70s in some kinetoplastid parasites, knowledge is still lacking pertaining to J-proteins and their partnerships with Hsp70s. This thesis focused on the classification of kinetoplastid Jproteins into the four types by examining the domain organizations using T. brucei as a guide. The potential partnership of J-proteins and Hsp70s were postulated based on predicted subcellular localization. Kinetoplastid parasites, particularly T. brucei, have evolved an expanded and specialized J-protein machinery, likely to be a consequence of an evolutionary fitness/trait to adapt to diverse environment present in hosts and vectors. These analyses will yield insight into the process of parasite differentiation as well as provide new leads for chemotherapeutic treatments. The presence of the STI1 mediated Hsp90 hetero-complex formation has not been confirmed in T. brucei. To this end, in silico and biochemical techniques were used to characterize the role of TbSTI1, as an adaptor protein of Hsp70 and Hsp90. Through domain architecture analysis, sequence alignments, phylogenetic analysis and three-dimensional structure prediction, TbSTI1 was demonstrated to be the most conserved TPR containing co-chaperone of Hsp70 and Hsp83 in T. brucei and also shown to be highly similar to its eukaryotic homologues. Recombinant TbSTI1 was overproduced and purified in E.coli cells and subsequently shown to associate with TcHsp70 in a concentration dependent manner and associate weakly with TbHsp70.4. TbSTI1 and TbHsp83 were also demonstrated to be expressed and upregulated upon exposure to heat shock at the bloodstream stage of parasite development. In conclusion, this study is the first to report the interaction of TbSTI1 with a chaperone. Interactions between TbSTI1 and Hsp70s were demonstrated and therefore, the formation of the hetero-complex is predicted based the similarity of TbSTI1 to other STI1 proteins.
- Full Text:
- Date Issued: 2015
- Authors: Mokoena, Fortunate
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54543 , vital:26583
- Description: African Trypanosomiasis, which is caused by Trypanosoma brucei, is one of the crippling agents of social and economic development in Africa. T. brucei cycles between the cold-blooded insect vector, the tsetse fly (Glossina spp), and warm-blooded mammalian hosts. T. brucei, T. cruzi and L. major are mammal infecting kinetoplastid parasites that are collectively referred to as TriTryps. These parasites experience extreme environments as they move between their warm-blooded mammalian hosts and cold-blooded insect vectors which trigger extensive morphological transformations during the life-cycle of the parasite. Molecular chaperones have been implicated in parasite differentiation. TriTryps display significant expansions and diversity in the gene complements encoding molecular chaperones, especially J-proteins. Generally, J-proteins function as co-chaperones of Hsp70s, forming part of vital protein homeostasis processes. Hsp70s show a high degree of conservation, while J-proteins appear to be an extreme case of taxonomic radiation. Although several studies have focused on the molecular and cell biology of Hsp70s in some kinetoplastid parasites, knowledge is still lacking pertaining to J-proteins and their partnerships with Hsp70s. This thesis focused on the classification of kinetoplastid Jproteins into the four types by examining the domain organizations using T. brucei as a guide. The potential partnership of J-proteins and Hsp70s were postulated based on predicted subcellular localization. Kinetoplastid parasites, particularly T. brucei, have evolved an expanded and specialized J-protein machinery, likely to be a consequence of an evolutionary fitness/trait to adapt to diverse environment present in hosts and vectors. These analyses will yield insight into the process of parasite differentiation as well as provide new leads for chemotherapeutic treatments. The presence of the STI1 mediated Hsp90 hetero-complex formation has not been confirmed in T. brucei. To this end, in silico and biochemical techniques were used to characterize the role of TbSTI1, as an adaptor protein of Hsp70 and Hsp90. Through domain architecture analysis, sequence alignments, phylogenetic analysis and three-dimensional structure prediction, TbSTI1 was demonstrated to be the most conserved TPR containing co-chaperone of Hsp70 and Hsp83 in T. brucei and also shown to be highly similar to its eukaryotic homologues. Recombinant TbSTI1 was overproduced and purified in E.coli cells and subsequently shown to associate with TcHsp70 in a concentration dependent manner and associate weakly with TbHsp70.4. TbSTI1 and TbHsp83 were also demonstrated to be expressed and upregulated upon exposure to heat shock at the bloodstream stage of parasite development. In conclusion, this study is the first to report the interaction of TbSTI1 with a chaperone. Interactions between TbSTI1 and Hsp70s were demonstrated and therefore, the formation of the hetero-complex is predicted based the similarity of TbSTI1 to other STI1 proteins.
- Full Text:
- Date Issued: 2015
Evaluation of knowledge and practices of nurses with regard to prevention of nosocomial infection in the intensive care units of Eastern Cape Province
- Mlenzana, Nosiphiwo Priscilla
- Authors: Mlenzana, Nosiphiwo Priscilla
- Date: 2015
- Subjects: Nosocomial infections http://id.loc.gov/authorities/subjects/sh85092770 , Cross infection--Prevention http://id.loc.gov/authorities/subjects/sh2009122612 , Infection http://id.loc.gov/authorities/subjects/sh85066076
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19639 , vital:43154
- Description: Aim: To determine the level of knowledge and practice of the nurses in the prevention and control of nosocomial infection in the intensive care units and make recommendations aiming at bridging the gap identified. In this study, the level of knowledge and practice of nurses in the intensive care units was described and its relationship with the demographic characteristics was examined utilising a quantitative approach. A quantitative descriptive design was used to examine and describe knowledge and practices of intensive care nurses with regards to prevention and control of nosocomial infection. One hundred and thirty (130) nurses participated in the study taken from the intensive care units of the five (5) state hospitals of the Eastern Cape Province. A self-designed questionnaire with close-ended questions was utilised comprising of dichotomous questions, with ‘yes’ or ‘no’ measuring knowledge nurses have and a Likert scale that measured knowledge with regards to clinical practice utilised in the prevention and control of nosocomial infections. The following ethical considerations were addressed by the study: ethical clearance, permission to conduct the study, informed consent from participants, anonymity and confidentiality and respect of individual autonomy. Findings: The respondents showed encouraging agreement with the different practices that should be followed in order to prevent spreading of nosocomial infections in their units. This knowledge suggests that the problem of infection control in ICUs may be related to the individual characteristics of the profession as opposed to being institutional. Limitations: Not all the intensive care units of the Eastern Cape hospitals were included due to challenges of time, finance and geographic factors. Recommendations: The following recommendations were made: Orientation programme to all new staff coming into the ICUs including infection control policies and guidelines; Involvement of the ICU staff in the development of infection control policies so as to ensure ownership and ultimate compliance; The employer must ensure that all resources for infection control and prevention are available and accessible to the health care workers at all times to ensure compliance. , Thesis (MCur) -- University of Fort Hare, 2015
- Full Text:
- Date Issued: 2015
- Authors: Mlenzana, Nosiphiwo Priscilla
- Date: 2015
- Subjects: Nosocomial infections http://id.loc.gov/authorities/subjects/sh85092770 , Cross infection--Prevention http://id.loc.gov/authorities/subjects/sh2009122612 , Infection http://id.loc.gov/authorities/subjects/sh85066076
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19639 , vital:43154
- Description: Aim: To determine the level of knowledge and practice of the nurses in the prevention and control of nosocomial infection in the intensive care units and make recommendations aiming at bridging the gap identified. In this study, the level of knowledge and practice of nurses in the intensive care units was described and its relationship with the demographic characteristics was examined utilising a quantitative approach. A quantitative descriptive design was used to examine and describe knowledge and practices of intensive care nurses with regards to prevention and control of nosocomial infection. One hundred and thirty (130) nurses participated in the study taken from the intensive care units of the five (5) state hospitals of the Eastern Cape Province. A self-designed questionnaire with close-ended questions was utilised comprising of dichotomous questions, with ‘yes’ or ‘no’ measuring knowledge nurses have and a Likert scale that measured knowledge with regards to clinical practice utilised in the prevention and control of nosocomial infections. The following ethical considerations were addressed by the study: ethical clearance, permission to conduct the study, informed consent from participants, anonymity and confidentiality and respect of individual autonomy. Findings: The respondents showed encouraging agreement with the different practices that should be followed in order to prevent spreading of nosocomial infections in their units. This knowledge suggests that the problem of infection control in ICUs may be related to the individual characteristics of the profession as opposed to being institutional. Limitations: Not all the intensive care units of the Eastern Cape hospitals were included due to challenges of time, finance and geographic factors. Recommendations: The following recommendations were made: Orientation programme to all new staff coming into the ICUs including infection control policies and guidelines; Involvement of the ICU staff in the development of infection control policies so as to ensure ownership and ultimate compliance; The employer must ensure that all resources for infection control and prevention are available and accessible to the health care workers at all times to ensure compliance. , Thesis (MCur) -- University of Fort Hare, 2015
- Full Text:
- Date Issued: 2015