Cost-effective, post-mining environmental restoration of an open-cast phosphate mine at Langebaanweg, South Africa
- Authors: Van Eeden, Joseph Deon
- Date: 2010
- Subjects: Abandoned mined lands reclamation -- South Africa -- Langebaan , Phosphate mines and mining -- Environmental aspects -- South Africa -- Langebaan , Phytoremediation -- South Africa -- Langebaan
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4232 , http://hdl.handle.net/10962/d1003801 , Abandoned mined lands reclamation -- South Africa -- Langebaan , Phosphate mines and mining -- Environmental aspects -- South Africa -- Langebaan , Phytoremediation -- South Africa -- Langebaan
- Description: Approaching the ecological rehabilitation of an open-cast phosphate mine in the West Coast of South Africa during the post-operational phase presented some challenges. The area was extensively modified during the mining operations. Soils from different layers were mixed with topsoil being covered by subsoil, overburden dumps and tailing dams being constructed resulting in extensive cross-zoned soils. Large areas of subsoil areas were exposed on the mine floor with localized and small scale salinity being evident. The modified topography as well as the complex new surface material posed a challenge in terms of identifying suitable local species that could be used to rehabilitate the post-mining environment. The mine area was heavily infested with woody alien invasive plants, such as Acacia cyclops, established in an attempt to reduce the dust and little natural vegetation cover was present. In the arid west coast environment, the four-month-long winter growing season is followed by hot and windy dry summers (Chapter 2) presenting a challenge reestablishing local vegetation in modified soils. Moreover, little was known about the local vegetation in terms of their propagation and use in stabilization techniques as an alternative to exotic vegetation such as the A. cyclops, which had been more often used in revegetation projects. A study was conducted to determine the most efficient and cost-effective methods of vegetative rehabilitation of the Chemfos site (Chapter 1). A review of the literature available at the time as well as approaches that were successfully implemented in other Western Cape rehabilitation projects such as the Du Toitskloof Pass and the Sishen–Saldanha railway line (Chapter 3), were considered. Previous studies on the west coast dunes at Blaauwberg had shown using Marram grass as a dune stabilizer was most successful. Marram grass was tested in trials alongside local grasses, such as Chaetobromus dregeanus and Ehrharta villosa, that showed potential but had not been formally evaluated. The environmental context of Chemfos (Chapter 2) as well as the Conceptual Rehabilitation Plan that considered the modified environment, soils and closure objectives of the mine, were reviewed to determine the most pressing rehabilitationrelated questions that required answers. This led to the final experimental design that was implemented mid-winter in 1996 (Chapter 5). The trials were implemented in the areas perceived to be the most difficult to rehabilitate, namely the mobile sands in the tailings dam and the exposed subsoil or mine floor areas. The use of brushwood together with specific plants and seeds appropriate for the use in either sandy soil or subsoil were evaluated in a variety of combinations and application densities to determine the most effective treatment combination at the minimum effective density. The best initial cover of the tailings dam were recorded in the Ammophila arenaria trials but the use of the local grass species Ehrharta villosa, performed better from year two onwards and was much cheaper to establish. On the subsoil, the trials where a cover of topsoil was used performed better than the combination trials. This indicated that topsoil placement on post-operational phase shaped subsoils during the mining operation to be the most desirable treatment. Initial results of the trials were used as a basis for developing the rehabilitation techniques that were rolled out across the Chemfos landscape. The approaches were refined as indications of trial responses became evident. Lessons learned were incorporated in the adaptive management approach that was followed and the rehabilitation techniques (Chapter 6) were continually re-evaluated and adjusted. This resulted in a significant step towards achieving the overall research objective of finding cost-effective approaches to rehabilitation. Components such as refinement of the seed collection and processing techniques (Chapter 7) where the post-harvest processing cost was significantly reduced by introducing specially designed drying racks. Processing techniques were adjusted to suit the different species, and a variety of mechanical processing options were explored. The scale of the Chemfos project led to the development of new techniques of manufacturing a smoke-derived germination stimulant (Chapter 8) since commercial availability of these products was very limited. The development of FireGrow assisted in the overall aim of reducing cost by increasing germination of seed in the rehabilitation sites using a very cost-effective smoke concentrate. Socio-economical aspects were considered during the implementation as well as the post-closure phases of the rehabilitation and BHPBilliton invested through the agency of the SAMANCOR Trust. This led to the development of livelihoods of the staff that remained in the area and that lived in the mine village. The demography of the population has changed over time in the Green Village as well as the skills that the inhabitants have developed. Thus, the new economic opportunities that were pursued have brought a new lease on life beyond the lifespan of the mine (Chapter 9) once the closure certificate had been issued.
- Full Text:
- Date Issued: 2010
- Authors: Van Eeden, Joseph Deon
- Date: 2010
- Subjects: Abandoned mined lands reclamation -- South Africa -- Langebaan , Phosphate mines and mining -- Environmental aspects -- South Africa -- Langebaan , Phytoremediation -- South Africa -- Langebaan
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4232 , http://hdl.handle.net/10962/d1003801 , Abandoned mined lands reclamation -- South Africa -- Langebaan , Phosphate mines and mining -- Environmental aspects -- South Africa -- Langebaan , Phytoremediation -- South Africa -- Langebaan
- Description: Approaching the ecological rehabilitation of an open-cast phosphate mine in the West Coast of South Africa during the post-operational phase presented some challenges. The area was extensively modified during the mining operations. Soils from different layers were mixed with topsoil being covered by subsoil, overburden dumps and tailing dams being constructed resulting in extensive cross-zoned soils. Large areas of subsoil areas were exposed on the mine floor with localized and small scale salinity being evident. The modified topography as well as the complex new surface material posed a challenge in terms of identifying suitable local species that could be used to rehabilitate the post-mining environment. The mine area was heavily infested with woody alien invasive plants, such as Acacia cyclops, established in an attempt to reduce the dust and little natural vegetation cover was present. In the arid west coast environment, the four-month-long winter growing season is followed by hot and windy dry summers (Chapter 2) presenting a challenge reestablishing local vegetation in modified soils. Moreover, little was known about the local vegetation in terms of their propagation and use in stabilization techniques as an alternative to exotic vegetation such as the A. cyclops, which had been more often used in revegetation projects. A study was conducted to determine the most efficient and cost-effective methods of vegetative rehabilitation of the Chemfos site (Chapter 1). A review of the literature available at the time as well as approaches that were successfully implemented in other Western Cape rehabilitation projects such as the Du Toitskloof Pass and the Sishen–Saldanha railway line (Chapter 3), were considered. Previous studies on the west coast dunes at Blaauwberg had shown using Marram grass as a dune stabilizer was most successful. Marram grass was tested in trials alongside local grasses, such as Chaetobromus dregeanus and Ehrharta villosa, that showed potential but had not been formally evaluated. The environmental context of Chemfos (Chapter 2) as well as the Conceptual Rehabilitation Plan that considered the modified environment, soils and closure objectives of the mine, were reviewed to determine the most pressing rehabilitationrelated questions that required answers. This led to the final experimental design that was implemented mid-winter in 1996 (Chapter 5). The trials were implemented in the areas perceived to be the most difficult to rehabilitate, namely the mobile sands in the tailings dam and the exposed subsoil or mine floor areas. The use of brushwood together with specific plants and seeds appropriate for the use in either sandy soil or subsoil were evaluated in a variety of combinations and application densities to determine the most effective treatment combination at the minimum effective density. The best initial cover of the tailings dam were recorded in the Ammophila arenaria trials but the use of the local grass species Ehrharta villosa, performed better from year two onwards and was much cheaper to establish. On the subsoil, the trials where a cover of topsoil was used performed better than the combination trials. This indicated that topsoil placement on post-operational phase shaped subsoils during the mining operation to be the most desirable treatment. Initial results of the trials were used as a basis for developing the rehabilitation techniques that were rolled out across the Chemfos landscape. The approaches were refined as indications of trial responses became evident. Lessons learned were incorporated in the adaptive management approach that was followed and the rehabilitation techniques (Chapter 6) were continually re-evaluated and adjusted. This resulted in a significant step towards achieving the overall research objective of finding cost-effective approaches to rehabilitation. Components such as refinement of the seed collection and processing techniques (Chapter 7) where the post-harvest processing cost was significantly reduced by introducing specially designed drying racks. Processing techniques were adjusted to suit the different species, and a variety of mechanical processing options were explored. The scale of the Chemfos project led to the development of new techniques of manufacturing a smoke-derived germination stimulant (Chapter 8) since commercial availability of these products was very limited. The development of FireGrow assisted in the overall aim of reducing cost by increasing germination of seed in the rehabilitation sites using a very cost-effective smoke concentrate. Socio-economical aspects were considered during the implementation as well as the post-closure phases of the rehabilitation and BHPBilliton invested through the agency of the SAMANCOR Trust. This led to the development of livelihoods of the staff that remained in the area and that lived in the mine village. The demography of the population has changed over time in the Green Village as well as the skills that the inhabitants have developed. Thus, the new economic opportunities that were pursued have brought a new lease on life beyond the lifespan of the mine (Chapter 9) once the closure certificate had been issued.
- Full Text:
- Date Issued: 2010
Developmental studies of certain South African Ascostromatic ascomycetes
- Authors: Tim, Stephen K-M
- Date: 1971
- Subjects: Fungi -- Parasites Ascomycetes Pyrenomycetes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4245 , http://hdl.handle.net/10962/d1007289
- Description: From General Introduction: Toward the last half of the nineteenth century, the structure and mode of development of the ascocarp has aroused much interest. De Bary (1887) recognised the fruiting bodies of the Ascomycetes as 'compound sporophores' made up of interwoven hyphae or of pseudoparenchyma consisting of a peripheral layer separate from an inner tissue. These fruiting bodies included the discocarp or apothecium, the pyrenocarp or perithecium and the cleistocarp or cleistothecium. The name, Pyrenomycetes, has been variously applied to a group of Ascomycetes but mainly to the perithecial types, inclusive of the true perithecia and the loculate forms. The perithecium itself was described as a 'cup-shaped discomycetous' sporocarp with margins incurved to form a pyriform structure (de Bary, 1887). The presence or absence of a stroma had been long considered as a basis for the separation of the major groups of the Ascomycetes. Separation on these grounds was found unacceptable as it grouped together clearly unrelated species or separated related ones. As the subdivisions of the pyrenomycetous Ascomycetes are dependent upon the morphological features of the ascocarp, it would be pertinent to discuss the following: a) The Stroma. b) The various forms of ascocarp which mayor may not be associated with such a stroma. i) Perithecia: free or immersed in a stroma. ii) Uni- or multiloculate stromata. c) The centrum, the details associated with the centrum and the use of these details in the taxonomy of the pyrenomycetes.
- Full Text:
- Date Issued: 1971
- Authors: Tim, Stephen K-M
- Date: 1971
- Subjects: Fungi -- Parasites Ascomycetes Pyrenomycetes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4245 , http://hdl.handle.net/10962/d1007289
- Description: From General Introduction: Toward the last half of the nineteenth century, the structure and mode of development of the ascocarp has aroused much interest. De Bary (1887) recognised the fruiting bodies of the Ascomycetes as 'compound sporophores' made up of interwoven hyphae or of pseudoparenchyma consisting of a peripheral layer separate from an inner tissue. These fruiting bodies included the discocarp or apothecium, the pyrenocarp or perithecium and the cleistocarp or cleistothecium. The name, Pyrenomycetes, has been variously applied to a group of Ascomycetes but mainly to the perithecial types, inclusive of the true perithecia and the loculate forms. The perithecium itself was described as a 'cup-shaped discomycetous' sporocarp with margins incurved to form a pyriform structure (de Bary, 1887). The presence or absence of a stroma had been long considered as a basis for the separation of the major groups of the Ascomycetes. Separation on these grounds was found unacceptable as it grouped together clearly unrelated species or separated related ones. As the subdivisions of the pyrenomycetous Ascomycetes are dependent upon the morphological features of the ascocarp, it would be pertinent to discuss the following: a) The Stroma. b) The various forms of ascocarp which mayor may not be associated with such a stroma. i) Perithecia: free or immersed in a stroma. ii) Uni- or multiloculate stromata. c) The centrum, the details associated with the centrum and the use of these details in the taxonomy of the pyrenomycetes.
- Full Text:
- Date Issued: 1971
Drought responses of C3 and C4 (NADP-ME) Panicoid grasses
- Authors: Frole, Kristen Marie
- Date: 2008
- Subjects: Botany -- Research , Grasses -- Physiology -- South Africa , Grasses -- Effect of drought on , Grasses -- Drought tolerance , Plant-water relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4193 , http://hdl.handle.net/10962/d1003762 , Botany -- Research , Grasses -- Physiology -- South Africa , Grasses -- Effect of drought on , Grasses -- Drought tolerance , Plant-water relationships
- Description: The success of C₄ plants lies in their ability to concentrate CO₂ at the site of Rubisco thereby conferring greater efficiencies of light, water and nitrogen. Such characteristics should advantage C₄ plants in arid, hot environments. However, not all C₄ subtypes are drought tolerant. The relative abundance of NADP-ME species declines with increasing aridity. Furthermore, selected species have been demonstrated as being susceptible to severe drought showing metabolic limitations of photosynthesis. However there is a lack of phylogenetic control with many of these studies. The aims of this study were to determine whether the NADP-ME subtype was inherently susceptible to drought by comparing six closely related C₃ and C₄ (NADP-ME) Panicoid grasses. Gas exchange measurements were made during a natural rainless period and a controlled drought / rewatering event. Prior to water stress, the C₄ species had higher assimilation rates (A), and water use efficiencies (WUE[subscript leaf]) than the C₃ species, while transpiration rates (E) and stomatal conductances (g[subscript s]) were similar. At low soil water content, the C₃ species reduced gs by a greater extent than the C₄ species, which maintained higher E during the driest periods. The C₄ species showed proportionally greater reductions in A than the C₃ species and hence lost their WUE[subscript leaf] and photosynthetic advantage. CO₂ response curves showed that metabolic limitation was responsible for a greater decrease in A in the C₄ type than the C₃ type during progressive drought. Upon re-watering, photosynthetic recovery was quicker in the C species than the C₄ species. Results from whole plant measurements showed that the C₄ type had a significant whole plant water use efficiency advantage over the C₃ type under well-watered conditions that was lost during severe drought due to a greater loss of leaf area through leaf mortality rather than reductions in plant level transpiration rates. The C₃ type had xylem characteristics that enhanced water-conducting efficiency, but made them vulnerable to drought. This is in contrast to the safer xylem qualities of the C₄ type, which permitted the endurance of more negative leaf water potentials than the C₃ type during low soil water content. Thus, the vulnerability of photosynthesis to severe drought in NADP-ME species potentially explains why NADP-ME species abundance around the world decreases with decreasing rainfall.
- Full Text:
- Date Issued: 2008
- Authors: Frole, Kristen Marie
- Date: 2008
- Subjects: Botany -- Research , Grasses -- Physiology -- South Africa , Grasses -- Effect of drought on , Grasses -- Drought tolerance , Plant-water relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4193 , http://hdl.handle.net/10962/d1003762 , Botany -- Research , Grasses -- Physiology -- South Africa , Grasses -- Effect of drought on , Grasses -- Drought tolerance , Plant-water relationships
- Description: The success of C₄ plants lies in their ability to concentrate CO₂ at the site of Rubisco thereby conferring greater efficiencies of light, water and nitrogen. Such characteristics should advantage C₄ plants in arid, hot environments. However, not all C₄ subtypes are drought tolerant. The relative abundance of NADP-ME species declines with increasing aridity. Furthermore, selected species have been demonstrated as being susceptible to severe drought showing metabolic limitations of photosynthesis. However there is a lack of phylogenetic control with many of these studies. The aims of this study were to determine whether the NADP-ME subtype was inherently susceptible to drought by comparing six closely related C₃ and C₄ (NADP-ME) Panicoid grasses. Gas exchange measurements were made during a natural rainless period and a controlled drought / rewatering event. Prior to water stress, the C₄ species had higher assimilation rates (A), and water use efficiencies (WUE[subscript leaf]) than the C₃ species, while transpiration rates (E) and stomatal conductances (g[subscript s]) were similar. At low soil water content, the C₃ species reduced gs by a greater extent than the C₄ species, which maintained higher E during the driest periods. The C₄ species showed proportionally greater reductions in A than the C₃ species and hence lost their WUE[subscript leaf] and photosynthetic advantage. CO₂ response curves showed that metabolic limitation was responsible for a greater decrease in A in the C₄ type than the C₃ type during progressive drought. Upon re-watering, photosynthetic recovery was quicker in the C species than the C₄ species. Results from whole plant measurements showed that the C₄ type had a significant whole plant water use efficiency advantage over the C₃ type under well-watered conditions that was lost during severe drought due to a greater loss of leaf area through leaf mortality rather than reductions in plant level transpiration rates. The C₃ type had xylem characteristics that enhanced water-conducting efficiency, but made them vulnerable to drought. This is in contrast to the safer xylem qualities of the C₄ type, which permitted the endurance of more negative leaf water potentials than the C₃ type during low soil water content. Thus, the vulnerability of photosynthesis to severe drought in NADP-ME species potentially explains why NADP-ME species abundance around the world decreases with decreasing rainfall.
- Full Text:
- Date Issued: 2008
Drought responses of selected C₄ photosynthetic NADP-Me and NAD-Me Panicoideae and Aristidoideae grasses
- Authors: Venter, Nicolaas
- Date: 2015
- Subjects: Aristida , Panicum , Switchgrass , Grasses -- Effect of drought on , Grasses -- Phylogeny , Photosynthesis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4272 , http://hdl.handle.net/10962/d1018549
- Description: Grass species within South Africa show a photosynthetic subtype and phylogenetic response to rainfall gradients, with Panicoideae species (NADP-Me and NAD-Me) inhabiting mesic environments, while Aristidoideae species (NADP-Me) inhabit more arid environments. It is predicted that climate change will alter rainfall patterns within southern Africa, which could have implications for grassland distributions and functional composition. Globally, and in South Africa, species distributions indicates that NAD-Me species have a preference for more arid environments, but this may be complicated by phylogeny as most NAD-Me species belong to the Chloridoideae subfamily. Additionally, differences in the metabolism and energetic requirements of different carboxylation types are expected to confer different ecological advantages, such as drought tolerance, but the role of these different pathways is not well understood. Based on natural distribution and photosynthetic subtype differences, it was hypothesised that Panicoideae NADP-Me species would be less drought tolerant than Panicoideae NAD-Me and Aristidoideae NADP-Me species and that subtypes and lineages would show different drought recovery rates. Furthermore, drought sensitivity would be of a metabolic and not a stomatal origin and plants that maintained favourable leaf water status would be more drought tolerant and recover faster. This was tested experimentally by comparing Panicoideae species (NADP-Me and NAD-Me) and NADP-Me species (Panicoideae and Aristidoideae). Plants were subjected to a progressive 58 day drought period and a recovery phase where gas exchange, chlorophyll fluorescence and leaf water relations were measured at select intervals. In conjunction with this, a rapid drought experiment was performed on Zea mays (NADP-Me: Panicoideae) plants where similar parameters were measured. Photosynthetic drought and recovery responses showed both a subtype and phylogenetic response. Panicoideae species were less drought tolerant than Aristidoideae species, although Panicoideae NAD-Me showed better recovery rates than Panicoideae NADP-Me species, while Aristidoideae species recovered the quickest. Panicoideae NAD-Me and Aristidoideae species maintained higher leaf water status during drought which contributed to the maintenance of PSII integrity and thus facilitated rapid photosynthetic recovery. During drought Panicoideae species showed greater metabolic limitations over Aristidoideae species and for the first time, lower metabolic limitations were associated with osmotic adjustment. This is a novel finding whereby osmotic adjustment and the subsequent maintenance of leaf water are key to preventing metabolic limitations of photosynthesis in C₄ grasses. Results from the Z. mays rapid drought study showed the limitations to photosynthesis were exclusively metabolic and unlikely to be a direct consequence of turgor loss. It was apparent that the response to drought was stronger amongst lineages, as NADP-Me species from different subfamilies showed a significant difference in drought tolerances. Aristidoideae species’ exceptional drought tolerance and predicted increased aridification could favour these species over Panicoideae species under future climates.
- Full Text:
- Date Issued: 2015
- Authors: Venter, Nicolaas
- Date: 2015
- Subjects: Aristida , Panicum , Switchgrass , Grasses -- Effect of drought on , Grasses -- Phylogeny , Photosynthesis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4272 , http://hdl.handle.net/10962/d1018549
- Description: Grass species within South Africa show a photosynthetic subtype and phylogenetic response to rainfall gradients, with Panicoideae species (NADP-Me and NAD-Me) inhabiting mesic environments, while Aristidoideae species (NADP-Me) inhabit more arid environments. It is predicted that climate change will alter rainfall patterns within southern Africa, which could have implications for grassland distributions and functional composition. Globally, and in South Africa, species distributions indicates that NAD-Me species have a preference for more arid environments, but this may be complicated by phylogeny as most NAD-Me species belong to the Chloridoideae subfamily. Additionally, differences in the metabolism and energetic requirements of different carboxylation types are expected to confer different ecological advantages, such as drought tolerance, but the role of these different pathways is not well understood. Based on natural distribution and photosynthetic subtype differences, it was hypothesised that Panicoideae NADP-Me species would be less drought tolerant than Panicoideae NAD-Me and Aristidoideae NADP-Me species and that subtypes and lineages would show different drought recovery rates. Furthermore, drought sensitivity would be of a metabolic and not a stomatal origin and plants that maintained favourable leaf water status would be more drought tolerant and recover faster. This was tested experimentally by comparing Panicoideae species (NADP-Me and NAD-Me) and NADP-Me species (Panicoideae and Aristidoideae). Plants were subjected to a progressive 58 day drought period and a recovery phase where gas exchange, chlorophyll fluorescence and leaf water relations were measured at select intervals. In conjunction with this, a rapid drought experiment was performed on Zea mays (NADP-Me: Panicoideae) plants where similar parameters were measured. Photosynthetic drought and recovery responses showed both a subtype and phylogenetic response. Panicoideae species were less drought tolerant than Aristidoideae species, although Panicoideae NAD-Me showed better recovery rates than Panicoideae NADP-Me species, while Aristidoideae species recovered the quickest. Panicoideae NAD-Me and Aristidoideae species maintained higher leaf water status during drought which contributed to the maintenance of PSII integrity and thus facilitated rapid photosynthetic recovery. During drought Panicoideae species showed greater metabolic limitations over Aristidoideae species and for the first time, lower metabolic limitations were associated with osmotic adjustment. This is a novel finding whereby osmotic adjustment and the subsequent maintenance of leaf water are key to preventing metabolic limitations of photosynthesis in C₄ grasses. Results from the Z. mays rapid drought study showed the limitations to photosynthesis were exclusively metabolic and unlikely to be a direct consequence of turgor loss. It was apparent that the response to drought was stronger amongst lineages, as NADP-Me species from different subfamilies showed a significant difference in drought tolerances. Aristidoideae species’ exceptional drought tolerance and predicted increased aridification could favour these species over Panicoideae species under future climates.
- Full Text:
- Date Issued: 2015
Ecology and degree of specialization of South African milkweeds with diverse pollination systems
- Authors: Coombs, Gareth
- Date: 2010
- Subjects: Milkweeds -- South Africa Milkweeds -- South Africa -- Ecology Milkweeds -- South Africa -- Pollination Allee effect Self-pollination Pollination by insects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4189 , http://hdl.handle.net/10962/d1003758
- Description: Like orchids, the complexity of flowers found in asclepiads (Asclepiadoideae, Apocynaceae) and the fact that pollen is presented as pollinaria, offers excellent opportunities to study various aspects of plant-pollinator interactions. In this thesis I investigated two broad themes: ecological aspects of the pollination biology of hymenopteran and fly-pollinated asclepiads as well as the degree of specialization to certain pollinators in these species. Colonizing plants often reproduce through self-pollination, or have highly generalized pollination systems, or both. These characteristics facilitate establishment in small founding populations and generates the prediction that reproductive success should be independent of population size in these species. Chapter one examines the pollination biology of Gomphocarpus physocarpus, an indigenous, weedy species and investigates the relationship between reproductive success and population size. In this species, there is no evidence of an Allee effect and reproductive success is not correlated with population size. In addition G. physocarpus is not capable of self-pollination, suggesting it is completely reliant on pollinators for seed set. The lack of a relationship between pollination success and population size is therefore likely explained by the generalized wasp pollination system of this species. Several milkweeds are invasive outside of their native ranges. Invasive species either need to co-opt native pollinators in order to reproduce or reduce their reliance on pollinators through having the ability to self-pollinate. Co-opting native pollinators is expected to be easier in species that have generalized pollination systems, alternatively species with specialized flower morphologies need to rely on similar functional groups of pollinators to be present within the invaded range. Chapter two investigates the pollination biology and pollination success of the invasive milkweed, Araujia sericifera, and finds that in South Africa, this species is visited mainly by native honeybees and nocturnal moths. Moths however contribute little to pollen removal, and deposition. Based on the apparent morphological mismatch between the flower of A. sericifera and native honeybees, I propose that the native pollinators of this species are likely to be larger Hymenoptera (e.g. Bumblebees). Data from a breeding system study, indicated that this species is not capable of automatic self-pollination, but could set fruit from geitonogamous self-pollinations pointing to the importance of native pollinators for successful reproduction. The pollinaria of milkweeds can accumulate on pollinators to form pollen masses large enough to physically interfere with the foraging behaviour of pollinating insects. In chapter three I describe the pollination biology of Cynanchum ellipticum and find that this species is mainly pollinated by honeybees although this species is visited by several other members of Hymenoptera, Lepidoptera and Diptera. Due to the structure of the pollinaria, these chain together relatively efficiently and frequently form large pollinarium loads on the mouthparts of honeybees. However there is little evidence that these pollinarium loads influence the foraging times of pollinators and only a few individual honeybees exhibited longer foraging times and most honeybees were unaffected by the presence of large pollinarium loads. Within the genus Cynanchum there is large variation in the gynostegium structure that may influence the pattern of pollinarium loading on pollinators as well as pollen reception as shown in chapter three. In Chapter four, the pollination biology of Cynanchum obtusifolium is examined, and like that of C. ellipticum, this species is visited by a wide diversity of pollinators but honeybees appear to be the primary pollinators. More importantly this species is shown to be andromonoecious and produces two morphologically different flower types, that may be distinguished based on differences in the gynostegium structure. These two types of flower could mainly be distinguished by the length of the anther wings. I found that flowers with short anther wings function as male flowers by only exporting- and rarely receiving pollinia. Flowers with longer anther wings function as hermaphrodite flowers and can both export and receive pollinia. The ratio of male to hermaphrodite flowers varied at different times during the flowering season, but preliminary data suggested that this was not related to levels of pollination success. The genera Stapelia and Ceropegia are well known for their intricate floral adaptations that mimic the brood and feeding substrates of pollinating flies. Despite several studies that have documented the various adaptations to fly pollination in different species, there is a lack of natural history studies documenting different flower visitors, pollen loads and long term levels of pollination success in these species. In Chapter six I document the pollination biology of Ceropegia ampliata by documenting different pollinators and quantifying average levels of pollination success and the nectar reward. I also experimentally manipulated the trapping hairs of this species to determine whether trapping hairs influence average levels of pollen export and receipt. I show that Ceropegia ampliata is pollinated by a generalist guild of flies (mainly Tachinidae, Sarcophagidae, Muscidae and Lauxaniidae) and produces minute quantities of relatively dilute nectar as a reward. Pollination success was generally low in this species and increases periodically suggesting that the abundance of pollinators is patchy. I found that flowers with trapping hairs that had already wilted had higher levels of pollinarium removal than flowers with erect hairs, however experimentally removing the hairs had no significant effect on pollen export and receipt. In Chapter seven, I document the pollinators, pollen loads and long term levels of pollination success in Stapelia hirsuta var. bayllissi, a rare sapromyiophilous stapeliad. I find that, in contrast to C. ampliata, this species was specialized to pollination by small flies of the family Anthomyiidae. Similar to the results from Chapter seven, I find that long term levels of pollination success were typically low but could increase periodically, although such increases were generally unpredictable. There are currently very few records documenting pollinator interactions in the Periplocoideae. Many species within this subfamily exhibit open-access flowers suggestive of pollination by short-tongued insects. I investigated the pollination biology of Chlorocyathus lobulata, a rare species with a highly localized distribution. I aimed to determine the pollinators, average levels of pollination success and demography of this species in order to determine whether this rare species is suffering from the collapse of a highly specialized pollinator mutualism. I also quantified the high incidence of flower herbivory caused by larvae of the moth, Bocchoris onychinalis. I find that C. lobulata has a highly generalized fly pollination system and average levels of pollination success suggested that a large proportion of flowers had pollen removed and deposited suggesting that this species is not experiencing pollination failure. The large numbers of juveniles present also indicated that recruitment is taking place.
- Full Text:
- Date Issued: 2010
- Authors: Coombs, Gareth
- Date: 2010
- Subjects: Milkweeds -- South Africa Milkweeds -- South Africa -- Ecology Milkweeds -- South Africa -- Pollination Allee effect Self-pollination Pollination by insects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4189 , http://hdl.handle.net/10962/d1003758
- Description: Like orchids, the complexity of flowers found in asclepiads (Asclepiadoideae, Apocynaceae) and the fact that pollen is presented as pollinaria, offers excellent opportunities to study various aspects of plant-pollinator interactions. In this thesis I investigated two broad themes: ecological aspects of the pollination biology of hymenopteran and fly-pollinated asclepiads as well as the degree of specialization to certain pollinators in these species. Colonizing plants often reproduce through self-pollination, or have highly generalized pollination systems, or both. These characteristics facilitate establishment in small founding populations and generates the prediction that reproductive success should be independent of population size in these species. Chapter one examines the pollination biology of Gomphocarpus physocarpus, an indigenous, weedy species and investigates the relationship between reproductive success and population size. In this species, there is no evidence of an Allee effect and reproductive success is not correlated with population size. In addition G. physocarpus is not capable of self-pollination, suggesting it is completely reliant on pollinators for seed set. The lack of a relationship between pollination success and population size is therefore likely explained by the generalized wasp pollination system of this species. Several milkweeds are invasive outside of their native ranges. Invasive species either need to co-opt native pollinators in order to reproduce or reduce their reliance on pollinators through having the ability to self-pollinate. Co-opting native pollinators is expected to be easier in species that have generalized pollination systems, alternatively species with specialized flower morphologies need to rely on similar functional groups of pollinators to be present within the invaded range. Chapter two investigates the pollination biology and pollination success of the invasive milkweed, Araujia sericifera, and finds that in South Africa, this species is visited mainly by native honeybees and nocturnal moths. Moths however contribute little to pollen removal, and deposition. Based on the apparent morphological mismatch between the flower of A. sericifera and native honeybees, I propose that the native pollinators of this species are likely to be larger Hymenoptera (e.g. Bumblebees). Data from a breeding system study, indicated that this species is not capable of automatic self-pollination, but could set fruit from geitonogamous self-pollinations pointing to the importance of native pollinators for successful reproduction. The pollinaria of milkweeds can accumulate on pollinators to form pollen masses large enough to physically interfere with the foraging behaviour of pollinating insects. In chapter three I describe the pollination biology of Cynanchum ellipticum and find that this species is mainly pollinated by honeybees although this species is visited by several other members of Hymenoptera, Lepidoptera and Diptera. Due to the structure of the pollinaria, these chain together relatively efficiently and frequently form large pollinarium loads on the mouthparts of honeybees. However there is little evidence that these pollinarium loads influence the foraging times of pollinators and only a few individual honeybees exhibited longer foraging times and most honeybees were unaffected by the presence of large pollinarium loads. Within the genus Cynanchum there is large variation in the gynostegium structure that may influence the pattern of pollinarium loading on pollinators as well as pollen reception as shown in chapter three. In Chapter four, the pollination biology of Cynanchum obtusifolium is examined, and like that of C. ellipticum, this species is visited by a wide diversity of pollinators but honeybees appear to be the primary pollinators. More importantly this species is shown to be andromonoecious and produces two morphologically different flower types, that may be distinguished based on differences in the gynostegium structure. These two types of flower could mainly be distinguished by the length of the anther wings. I found that flowers with short anther wings function as male flowers by only exporting- and rarely receiving pollinia. Flowers with longer anther wings function as hermaphrodite flowers and can both export and receive pollinia. The ratio of male to hermaphrodite flowers varied at different times during the flowering season, but preliminary data suggested that this was not related to levels of pollination success. The genera Stapelia and Ceropegia are well known for their intricate floral adaptations that mimic the brood and feeding substrates of pollinating flies. Despite several studies that have documented the various adaptations to fly pollination in different species, there is a lack of natural history studies documenting different flower visitors, pollen loads and long term levels of pollination success in these species. In Chapter six I document the pollination biology of Ceropegia ampliata by documenting different pollinators and quantifying average levels of pollination success and the nectar reward. I also experimentally manipulated the trapping hairs of this species to determine whether trapping hairs influence average levels of pollen export and receipt. I show that Ceropegia ampliata is pollinated by a generalist guild of flies (mainly Tachinidae, Sarcophagidae, Muscidae and Lauxaniidae) and produces minute quantities of relatively dilute nectar as a reward. Pollination success was generally low in this species and increases periodically suggesting that the abundance of pollinators is patchy. I found that flowers with trapping hairs that had already wilted had higher levels of pollinarium removal than flowers with erect hairs, however experimentally removing the hairs had no significant effect on pollen export and receipt. In Chapter seven, I document the pollinators, pollen loads and long term levels of pollination success in Stapelia hirsuta var. bayllissi, a rare sapromyiophilous stapeliad. I find that, in contrast to C. ampliata, this species was specialized to pollination by small flies of the family Anthomyiidae. Similar to the results from Chapter seven, I find that long term levels of pollination success were typically low but could increase periodically, although such increases were generally unpredictable. There are currently very few records documenting pollinator interactions in the Periplocoideae. Many species within this subfamily exhibit open-access flowers suggestive of pollination by short-tongued insects. I investigated the pollination biology of Chlorocyathus lobulata, a rare species with a highly localized distribution. I aimed to determine the pollinators, average levels of pollination success and demography of this species in order to determine whether this rare species is suffering from the collapse of a highly specialized pollinator mutualism. I also quantified the high incidence of flower herbivory caused by larvae of the moth, Bocchoris onychinalis. I find that C. lobulata has a highly generalized fly pollination system and average levels of pollination success suggested that a large proportion of flowers had pollen removed and deposited suggesting that this species is not experiencing pollination failure. The large numbers of juveniles present also indicated that recruitment is taking place.
- Full Text:
- Date Issued: 2010
Effects of sustained elevated CO2 concentration and Nitrogen nutrition on wheat (Triticum aestivum L. cv Gamtoos)
- Authors: Kgope, Barney Stephen
- Date: 2000
- Subjects: Wheat -- Growth , Wheat -- Nutrition , Nitrogen fertilizers , Nitrogen in agriculture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4205 , http://hdl.handle.net/10962/d1003774 , Wheat -- Growth , Wheat -- Nutrition , Nitrogen fertilizers , Nitrogen in agriculture
- Description: There is consensus that high CO2 results in enhanced growth and yield for most crop plants. However, most of these studies were carried out in the presence of adequate nutrients, which is also the case in agricultural systems (managed ecosystems). About 20% of the earth’s land mass have sufficiently low levels of nutrients to cause some kind of stress to plants. On the other hand, elevated [CO2] decreases foliar nutrient elements in plants and as a result partitioning of certain nutrient elements in plants is altered. Little data is available on the partitioning of most nutrient elements in plants, and this will definitely impact on growth and yield. To investigate this, wheat (Triticum aestivum L. c.v. Gamtoos) was grown in controlled environment cabinets at 360 and 700 µmol mol -1 CO2. The full Long-Ashton nutrient solution comprising of three-nitrogen concentrations ([N]) viz. (4,6 and 12 mM) was used to water plants everyday. The measurement of net assimilation rate (NAR), stomatal conductance (gs), transpiration rate (E), water use efficiency (WUE), foliar [N], nitrogen use efficiency (NUE) and growth parameters (total plant biomass (TPB), total plant height (TPH), leaf area (LA), shoot and root dry weight) were made 7 days after germination (7 DAG) till the onset of flowering. The increase in nitrogen supply in the order of 4, 6 and 12mM resulted in an increase in NAR, g_s_ , WUE and a decline in E under elevated [CO2]. Under elevated [CO2] NAR was observed to increase during the first two weeks reaching its maximum at 14 DAG, thereafter followed by a decline reaching its maximum at 28 DAG. This was later followed by an increase at 35 DAG onwards. Under elevated [CO2], NAR was increased significantly between the nitrogen regimes during the first (7-14 DAG) and the last two (35-42 DAG) weeks. The response of assimilation as a function of internal [CO2] (Ci), showed a decrease with age at ages 14, 28 and 35 DAG. This negatively affected the initial slope and the CO2 saturated photosynthetic rates under all treatments. This suggest that acclimation may have been as a result of both stomatal and biochemical limitations. All the photosynthetic pigment levels (chl_a_, chl_b_, chl_(a+b)_, and C_(x+c)_ ) increased with an increase in nitrogen supply from 4 to 6mM [N]. A 12mM [N] resulted in a significant decline in the photosynthetic pigment levels compared to a 6mM [N]. Chla remained higher than chlb under all treatments. Also, NAR was seen to increase and decrease concomitantly with the photosynthetic pigment levels. Foliar [N] was seen to decrease with an increase in nitrogen supply from 4 to 6 mM [N] under elevated [CO2] and the effects were adverse under the 4mM [N]. Under the 6mM N regime foliar [N] was positively correlated to NAR for elevated [CO2] grown plants. Similarly, E was positively correlated to foliar [N] under the same conditions. Elevated CO2 and increase in nitrogen supply had a pronounced effect on total plant height (TPH), total plant biomass (TPB), leaf area (LA), shoot and root dry weight and nitrogen use efficiency (NUE). The effects were more pronounced under a 6mM [N] as a result of high NUE. However, under 12mM [N] growth was not as expected as a result of lower NUE. Under all treatments shoot dry weight (SDW) was positively correlated to NUE. Anatomical studies revealed that total leaf and midrib thickness was significantly increased with an increase in nitrogen supply under elevated CO2 to support the larger leaf areas. There were no significant changes in the chloroplast ultrastructure as a result of the increase in nitrogen supply and CO2 enrichment. Starch grain surface area was seen to decline with an increase in nitrogen under both ambient and elevated CO2. Elevated CO2 and increase in nitrogen supply significantly increased total grain dry weight per plant by 47 and 46% respectively under 6 and 12mM [N]. In contrast, the increase was by about 21, 61 and 67% respectively under 4, 6 and 12mM [N] between the CO2 regimes.
- Full Text:
- Date Issued: 2000
- Authors: Kgope, Barney Stephen
- Date: 2000
- Subjects: Wheat -- Growth , Wheat -- Nutrition , Nitrogen fertilizers , Nitrogen in agriculture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4205 , http://hdl.handle.net/10962/d1003774 , Wheat -- Growth , Wheat -- Nutrition , Nitrogen fertilizers , Nitrogen in agriculture
- Description: There is consensus that high CO2 results in enhanced growth and yield for most crop plants. However, most of these studies were carried out in the presence of adequate nutrients, which is also the case in agricultural systems (managed ecosystems). About 20% of the earth’s land mass have sufficiently low levels of nutrients to cause some kind of stress to plants. On the other hand, elevated [CO2] decreases foliar nutrient elements in plants and as a result partitioning of certain nutrient elements in plants is altered. Little data is available on the partitioning of most nutrient elements in plants, and this will definitely impact on growth and yield. To investigate this, wheat (Triticum aestivum L. c.v. Gamtoos) was grown in controlled environment cabinets at 360 and 700 µmol mol -1 CO2. The full Long-Ashton nutrient solution comprising of three-nitrogen concentrations ([N]) viz. (4,6 and 12 mM) was used to water plants everyday. The measurement of net assimilation rate (NAR), stomatal conductance (gs), transpiration rate (E), water use efficiency (WUE), foliar [N], nitrogen use efficiency (NUE) and growth parameters (total plant biomass (TPB), total plant height (TPH), leaf area (LA), shoot and root dry weight) were made 7 days after germination (7 DAG) till the onset of flowering. The increase in nitrogen supply in the order of 4, 6 and 12mM resulted in an increase in NAR, g_s_ , WUE and a decline in E under elevated [CO2]. Under elevated [CO2] NAR was observed to increase during the first two weeks reaching its maximum at 14 DAG, thereafter followed by a decline reaching its maximum at 28 DAG. This was later followed by an increase at 35 DAG onwards. Under elevated [CO2], NAR was increased significantly between the nitrogen regimes during the first (7-14 DAG) and the last two (35-42 DAG) weeks. The response of assimilation as a function of internal [CO2] (Ci), showed a decrease with age at ages 14, 28 and 35 DAG. This negatively affected the initial slope and the CO2 saturated photosynthetic rates under all treatments. This suggest that acclimation may have been as a result of both stomatal and biochemical limitations. All the photosynthetic pigment levels (chl_a_, chl_b_, chl_(a+b)_, and C_(x+c)_ ) increased with an increase in nitrogen supply from 4 to 6mM [N]. A 12mM [N] resulted in a significant decline in the photosynthetic pigment levels compared to a 6mM [N]. Chla remained higher than chlb under all treatments. Also, NAR was seen to increase and decrease concomitantly with the photosynthetic pigment levels. Foliar [N] was seen to decrease with an increase in nitrogen supply from 4 to 6 mM [N] under elevated [CO2] and the effects were adverse under the 4mM [N]. Under the 6mM N regime foliar [N] was positively correlated to NAR for elevated [CO2] grown plants. Similarly, E was positively correlated to foliar [N] under the same conditions. Elevated CO2 and increase in nitrogen supply had a pronounced effect on total plant height (TPH), total plant biomass (TPB), leaf area (LA), shoot and root dry weight and nitrogen use efficiency (NUE). The effects were more pronounced under a 6mM [N] as a result of high NUE. However, under 12mM [N] growth was not as expected as a result of lower NUE. Under all treatments shoot dry weight (SDW) was positively correlated to NUE. Anatomical studies revealed that total leaf and midrib thickness was significantly increased with an increase in nitrogen supply under elevated CO2 to support the larger leaf areas. There were no significant changes in the chloroplast ultrastructure as a result of the increase in nitrogen supply and CO2 enrichment. Starch grain surface area was seen to decline with an increase in nitrogen under both ambient and elevated CO2. Elevated CO2 and increase in nitrogen supply significantly increased total grain dry weight per plant by 47 and 46% respectively under 6 and 12mM [N]. In contrast, the increase was by about 21, 61 and 67% respectively under 4, 6 and 12mM [N] between the CO2 regimes.
- Full Text:
- Date Issued: 2000
Effects of sustained elevated CO₂ concentration on two cultivars of barley (Hordeum vulgare L.)
- Authors: Siphugu, Mashudu Victor
- Date: 1997
- Subjects: Barley Barley -- Growth Photosynthesis -- Research Plants -- Effect of carbon dioxide on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4226 , http://hdl.handle.net/10962/d1003795
- Description: The enormous burning of fossil fuel and deforestation have caused an increase in the atmospheric CO₂ concentration ([CO₂]) during the last century. This will invariably have profound direct and indirect effects on plant carbon metabolism. The majority of research on the effects of CO₂ enrichment on plants are short-term and are done on other crops, but very little have been done on barley. This project aimed to determine the effects of long-term CO₂ enrichment on photosynthesis, growth and grain yield on barley. Hordeum vulgare L. cvs Stirling and Schooner plants were grown from seeds in controlled environment chambers at ambient (350) and elevated (600) μmol molˉ¹ [CO₂]. Measurements of net assimilation rate (NAR), photosynthetic pigments content and growth parameters were started 7 days after germination (DAG) and continued until senescence. The anatomy of matured fully developed leaves was also monitored. Elevated [CO₂l resulted in an increase in NAR in the two cultivars from days 7 until 14, after which the stimulation of NAR of CO₂-enriched plants started to decrease. At the onset of senescence, NAR was almost equal in plants grown under both ambient and elevated [CO₂]. The response of assimilation as a function of internal [CO₂l (C₁) at the end of the experimental period showed a significant decrease in both the initial slope of the A/C₁ curves and the CO₂-saturated photosynthetic rates in the two cultivars. Stirling showed no significant changes in the content of chlorophyll α,chlorophyll б or in total carotenoids. However, Schooner showed a stimulation in chlorophyll α content at day 7, but decreased at day 28. Chlorophyll б and total carotenoids content were not affected by CO₂ enrichment. While total above-ground biomass was not affected by elevated [CO₂] in the two cultivars, total plant height decreased significantly after 14 days in Stirling whereas no significant change occurred in Schooner throughout the experimental period. Leaf area was not significantly affected by CO₂ enrichment in the two cultivars although the leaves in CO₂ enriched plants were slightly shorter. Anatomical studies reveal that leaf thickness was significantly increased by CO₂ enrichment in Stirling, but the increase was not significant in Schooner. Both cultivars did not show any significant effect on chloroplast morphology and ultrastructure as a consequence of elevated CO₂ exposure. No signs of starch accumulation were evident in variety Schooner, but Stirling showed some form of starch accumulation, under increased atmospheric [CO₂]. Elevated CO₂ resulted in a significant reduction by more than 50 % in the number of grain yield per plant in both Stirling and Schooner. Results from this study therefore indicate that CO₂ enrichment will not be beneficial in terms of growth and yield in this important crop.
- Full Text:
- Date Issued: 1997
- Authors: Siphugu, Mashudu Victor
- Date: 1997
- Subjects: Barley Barley -- Growth Photosynthesis -- Research Plants -- Effect of carbon dioxide on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4226 , http://hdl.handle.net/10962/d1003795
- Description: The enormous burning of fossil fuel and deforestation have caused an increase in the atmospheric CO₂ concentration ([CO₂]) during the last century. This will invariably have profound direct and indirect effects on plant carbon metabolism. The majority of research on the effects of CO₂ enrichment on plants are short-term and are done on other crops, but very little have been done on barley. This project aimed to determine the effects of long-term CO₂ enrichment on photosynthesis, growth and grain yield on barley. Hordeum vulgare L. cvs Stirling and Schooner plants were grown from seeds in controlled environment chambers at ambient (350) and elevated (600) μmol molˉ¹ [CO₂]. Measurements of net assimilation rate (NAR), photosynthetic pigments content and growth parameters were started 7 days after germination (DAG) and continued until senescence. The anatomy of matured fully developed leaves was also monitored. Elevated [CO₂l resulted in an increase in NAR in the two cultivars from days 7 until 14, after which the stimulation of NAR of CO₂-enriched plants started to decrease. At the onset of senescence, NAR was almost equal in plants grown under both ambient and elevated [CO₂]. The response of assimilation as a function of internal [CO₂l (C₁) at the end of the experimental period showed a significant decrease in both the initial slope of the A/C₁ curves and the CO₂-saturated photosynthetic rates in the two cultivars. Stirling showed no significant changes in the content of chlorophyll α,chlorophyll б or in total carotenoids. However, Schooner showed a stimulation in chlorophyll α content at day 7, but decreased at day 28. Chlorophyll б and total carotenoids content were not affected by CO₂ enrichment. While total above-ground biomass was not affected by elevated [CO₂] in the two cultivars, total plant height decreased significantly after 14 days in Stirling whereas no significant change occurred in Schooner throughout the experimental period. Leaf area was not significantly affected by CO₂ enrichment in the two cultivars although the leaves in CO₂ enriched plants were slightly shorter. Anatomical studies reveal that leaf thickness was significantly increased by CO₂ enrichment in Stirling, but the increase was not significant in Schooner. Both cultivars did not show any significant effect on chloroplast morphology and ultrastructure as a consequence of elevated CO₂ exposure. No signs of starch accumulation were evident in variety Schooner, but Stirling showed some form of starch accumulation, under increased atmospheric [CO₂]. Elevated CO₂ resulted in a significant reduction by more than 50 % in the number of grain yield per plant in both Stirling and Schooner. Results from this study therefore indicate that CO₂ enrichment will not be beneficial in terms of growth and yield in this important crop.
- Full Text:
- Date Issued: 1997
Effects of sustained Russian wheat aphid (Diuraphis noxia Mordvilko) feeding on leaf blades of wheat (Triticum aestivum L. cv Adamtas)
- Authors: Matsiliza, Babalwa
- Date: 2003
- Subjects: Russian wheat aphid Wheat -- Diseases and pests
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4213 , http://hdl.handle.net/10962/d1003782
- Description: Penetration of sink as well as source leaves of wheat plants by the Russian wheat aphid, Diuraphis noxia (Mordvilko) was investigated using light, fluorescence and transmission electron techniques, to determine the feeding strategies adopted by the aphid in penetrating and successfully feeding from the phloem, and to assess the structural effects of the probing and feeding behaviour of D. noxia on the feeding sites. Examination of aphid-infested sink, as well as source leaf tissue, showed that D.noxia probed in cells of the vascular bundle more frequently than mesophyll cells. Within the vascular bundle, thin-walled sieve tubes were visited (probed) more than the other cells. In sink leaf material, 68 of 82 (83%) stylets and stylet tracks encountered during the examination of 1000 serial sections (from 5 different plants) terminated in thin-walled sieve tubes and only 14 (17%) in thick-walled sieve tubes. Thin-walled sieve tubes were visited more significantly than thick-walled sieve tubes. However, examination of the aphid.,.infested sink leaf on a per centimetre basis, from the tip of the leaf, revealed that thick-walled sieve tubes in the area closest to the tip (0-2cm from the tip) were as attractive to the aphid as were thin-walled sieve tubes, with no significant difference in the number of times thick- and thin-walled sieve tubes were probed in this area. Some 2-4cm from the tip however, thinwalled sieve tubes were significantly more probed and therefore more attractive than thick-walled sieve tubes. Examination of 2000 serial sections using aphid-infested source leaf tissue, showed that the thin-walled sieve tubes were significantly more probed than thickwalled sieve tubes, along the whole leaf, expressed as a total of all leaves, as well as on a per centimetre basis along the length of the leaf, with 212 (95%) of 222 terminations within the thin-walled sieve tubes and only 10 (5%) in thick-walledsieve tubes. The aphid probed the small vascular bundles (loading bundles) many more times than intermediate or large transport vascular bundles, in sink as well as source leaf. Of a total of 82 stylets and stylet tracks encountered in sink leaf tissue, 31 terminated in small vascular bundles ang the remaining 28 and 16 were located within large and intermediate vascular bundles respectively. In source leaf tissue 121 of 222 stylets and stylet tracks encountered were associated with small vascular bundles and only 58 tracks and 43 tracks with intermediate and large vascular bundles, respectively. The effect of sustained RWA feeding on the transport capacity was examined after the application of 5,6 carboxyfluoresceine diacetate (5,6-CFDA) in control (sink and source leaf tissue) and aphid-infested (source) wheat leaves, using fluorescence microscopy. After 3h acropetal longitudinal transport of 5,6-CF had occurred in sink leaves in longitudinal veins, as well as a lateral transfer via cross veins and subsequent unloading into mesophyll cells close to the tip of the leaf was observed. In control leaf tissue, the fluorescence front was detected up to about 5cm from the point of application and was only associated with the phloem and not unloaded. In contrast, aphid-infested leaf tissue showed very little 5,6-CF transport, being limited to 2cm or less from the point of application. Structural damage to the phloem in general and to the sieve tubes in particular within of control and infested wheat leaves was investigated using transmission electron microscopy (TEM). In addition, leaf strips were mounted in aniline blue to visualise callose deposition using the fluorescence microscopy. At the TEM level. infested leaf tissue showed various abnormalities, which included destruction of cell contents, membrane damage and subsequent loss of cell contents. TEM studies suggest severe osmotic shock resulted from the aphid's probing. Examination of leaf tissue using fluorescence microscopy showed that there was very little characteristic aniline blue-stained callose visible in control leaf tissue, other than the thin diffuse patches along the sieve plates and punctate spots associated with pore plasmodesmatal areas and plasmodesmatal aggregates. In contrast, the aphid-infested leaf tissue was heavily callosed, with callose deposited not only within the phloem tissue but also in neighbouring vascular parE:}nchyma cells as well. The data collectively suggest that D. noxia feeds preferentially within thin-walled sieve tubes, within the small longitudinal vascular bundles in sink , as well source leaf tissue. Based upon the data presented here the thin-walled sieve tubes in the wheat leaf appear to be more attractive to the aphid and that they are probably more functional in terms of transport system and unlo?lding in sink leaves. Aniline blue stained leaf material that had previously hosted large aphid colonies showed evidence of extensive callose deposits 24 to 36h after the aphids were removed, suggesting that the aphids caused severe mechanical damage to the vascular tissue and mesohyll cells as well. Damage (transient or more permanent) and the subsequent deposition of wound callose, disrupted phloem transport and hence the export of photoassimilate from the leaves.
- Full Text:
- Date Issued: 2003
- Authors: Matsiliza, Babalwa
- Date: 2003
- Subjects: Russian wheat aphid Wheat -- Diseases and pests
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4213 , http://hdl.handle.net/10962/d1003782
- Description: Penetration of sink as well as source leaves of wheat plants by the Russian wheat aphid, Diuraphis noxia (Mordvilko) was investigated using light, fluorescence and transmission electron techniques, to determine the feeding strategies adopted by the aphid in penetrating and successfully feeding from the phloem, and to assess the structural effects of the probing and feeding behaviour of D. noxia on the feeding sites. Examination of aphid-infested sink, as well as source leaf tissue, showed that D.noxia probed in cells of the vascular bundle more frequently than mesophyll cells. Within the vascular bundle, thin-walled sieve tubes were visited (probed) more than the other cells. In sink leaf material, 68 of 82 (83%) stylets and stylet tracks encountered during the examination of 1000 serial sections (from 5 different plants) terminated in thin-walled sieve tubes and only 14 (17%) in thick-walled sieve tubes. Thin-walled sieve tubes were visited more significantly than thick-walled sieve tubes. However, examination of the aphid.,.infested sink leaf on a per centimetre basis, from the tip of the leaf, revealed that thick-walled sieve tubes in the area closest to the tip (0-2cm from the tip) were as attractive to the aphid as were thin-walled sieve tubes, with no significant difference in the number of times thick- and thin-walled sieve tubes were probed in this area. Some 2-4cm from the tip however, thinwalled sieve tubes were significantly more probed and therefore more attractive than thick-walled sieve tubes. Examination of 2000 serial sections using aphid-infested source leaf tissue, showed that the thin-walled sieve tubes were significantly more probed than thickwalled sieve tubes, along the whole leaf, expressed as a total of all leaves, as well as on a per centimetre basis along the length of the leaf, with 212 (95%) of 222 terminations within the thin-walled sieve tubes and only 10 (5%) in thick-walledsieve tubes. The aphid probed the small vascular bundles (loading bundles) many more times than intermediate or large transport vascular bundles, in sink as well as source leaf. Of a total of 82 stylets and stylet tracks encountered in sink leaf tissue, 31 terminated in small vascular bundles ang the remaining 28 and 16 were located within large and intermediate vascular bundles respectively. In source leaf tissue 121 of 222 stylets and stylet tracks encountered were associated with small vascular bundles and only 58 tracks and 43 tracks with intermediate and large vascular bundles, respectively. The effect of sustained RWA feeding on the transport capacity was examined after the application of 5,6 carboxyfluoresceine diacetate (5,6-CFDA) in control (sink and source leaf tissue) and aphid-infested (source) wheat leaves, using fluorescence microscopy. After 3h acropetal longitudinal transport of 5,6-CF had occurred in sink leaves in longitudinal veins, as well as a lateral transfer via cross veins and subsequent unloading into mesophyll cells close to the tip of the leaf was observed. In control leaf tissue, the fluorescence front was detected up to about 5cm from the point of application and was only associated with the phloem and not unloaded. In contrast, aphid-infested leaf tissue showed very little 5,6-CF transport, being limited to 2cm or less from the point of application. Structural damage to the phloem in general and to the sieve tubes in particular within of control and infested wheat leaves was investigated using transmission electron microscopy (TEM). In addition, leaf strips were mounted in aniline blue to visualise callose deposition using the fluorescence microscopy. At the TEM level. infested leaf tissue showed various abnormalities, which included destruction of cell contents, membrane damage and subsequent loss of cell contents. TEM studies suggest severe osmotic shock resulted from the aphid's probing. Examination of leaf tissue using fluorescence microscopy showed that there was very little characteristic aniline blue-stained callose visible in control leaf tissue, other than the thin diffuse patches along the sieve plates and punctate spots associated with pore plasmodesmatal areas and plasmodesmatal aggregates. In contrast, the aphid-infested leaf tissue was heavily callosed, with callose deposited not only within the phloem tissue but also in neighbouring vascular parE:}nchyma cells as well. The data collectively suggest that D. noxia feeds preferentially within thin-walled sieve tubes, within the small longitudinal vascular bundles in sink , as well source leaf tissue. Based upon the data presented here the thin-walled sieve tubes in the wheat leaf appear to be more attractive to the aphid and that they are probably more functional in terms of transport system and unlo?lding in sink leaves. Aniline blue stained leaf material that had previously hosted large aphid colonies showed evidence of extensive callose deposits 24 to 36h after the aphids were removed, suggesting that the aphids caused severe mechanical damage to the vascular tissue and mesohyll cells as well. Damage (transient or more permanent) and the subsequent deposition of wound callose, disrupted phloem transport and hence the export of photoassimilate from the leaves.
- Full Text:
- Date Issued: 2003
Effects of treatment on Lantana camara (L.) and the restoration potential of riparian seed banks in cleared areas of the Victoria Falls World Heritage Site, Livingstone, Zambia
- Nang'alelwa, Michael Mubitelela
- Authors: Nang'alelwa, Michael Mubitelela
- Date: 2010
- Subjects: Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4217 , http://hdl.handle.net/10962/d1003786 , Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Description: The exotic plant Lantana camara L. has invaded the riparian areas of the Victoria Falls World Heritage Site in Livingstone, southern Zambia, threatening native plant communities which support populations of species of special concern. I trialled the mechanical control method of manual uprooting and 3 different herbicides applied through paint brushing of an imazapyr concentrate at 250g. l¯¹, spraying on cut stumps with metsulfron methyl at 600g.l¯¹, and foliar spraying on re-emergent lantana foliage with glyphosate at a dosage of 166g. l¯¹ in July 2008 in 20 100m2 treatment plots, 5 invaded control plots and 5 uninvaded controls. Follow-up treatments for re-sprouting lantana stumps and emerging seedlings were undertaken in June 2009. I measured effectiveness of the methods using adult lantana mortality in June 2009 and lantana seedling density in the different treatment plots during the follow-up exercise. The cost of the various methods and human labour applied were compared across the four treatments at initial clear and at follow-up. All treatments recorded a high adult lantana mortality rate, though there were no significant differences in lantana adult mortality amongst the treatments. Overall, uprooting had the highest adult mortality, followed by imazapyr, metsulfron and lastly glyphosate. Germination of lantana seedlings after clearing was high for all treatments but with no significant differences occurring between the treatments. Both adult lantana mortality and seedling density were however significantly different from the control. With labour included, chemical costs were far higher relative to uprooting, though uprooting costs were the highest when it came to the follow-up because of the emerging seedlings and some resprouting stumps. The effects of mechanical and chemical treatments on vegetation composition in the cleared areas were also assessed in order to detect any non-target and medium term effects of treatments. Contrary to expectation, none of the chemicals showed any significant effects on vegetation composition in the short and medium-term and no significant differences were found in plant species richness, diversity and seedling density between invaded and uninvaded plots at baseline, in October 2008 and in September 2009. In order to determine potential for unaided vegetation recovery in the riparian areas of the study site after lantana clearing, I conducted an investigation of soil seed banks and seed rain using 60 seed bank samples measuring 1800m³ collected from 30 invaded and uninvaded plots. Using the seedling emergence method, 1, 991 seedlings belonging to 66 species representing 27 families germinated from the seed bank. Sedges (Cyperaceae family) were the most abundant taxa in the seed banks from invaded areas, followed by Ageratum conyzoides, lantana, Triumfetta annua and Achyranthes aspera which also occurred in the uninvaded soil seed banks. The seed banks from uninvaded plots were dominated by the grass Oplismenus hirtellus. Overall, species richness, diversity and seedling density from seed banks in invaded areas did not differ significantly from seed bank in uninvaded areas and there was a low similarity in species composition when above ground vegetation was compared to seed banks from invaded and uninvaded areas. It would appear if natural regeneration occured from the current seed bank in disturbed areas, future vegetation would largely comprise of short lived, early successional species in the short term as the seed bank is dominated by non-native herbaceous weedy species. From the seed traps investigating seed rain, a total of 27 species numbering 623 individual seeds were found in the thirty 1m² seedtraps distributed in invaded and uninvaded areas at the five sites, over an intermittent period of three months. Lantana had the highest monthly arrival rate in the seed traps followed by Phoenix reclinata and Ricinus communis. The number of species with invasive potential found in the seed traps located in invaded areas was more than that found in seed traps under native vegetation cover by far. Considerable forest remnants still occur around the invaded sites, and these could serve as an important source for long-term natural re-establishment of native vegetation if seed availability by animals and wind dispersal continues, while the re-invasion of lantana is prevented by ongoing follow-ups and futher clearing of lantana invaded areas. It is concluded that while uprooting and other treatments are effective in the control of lantana, its successful control in the Victoria Falls World Heritage Site will require extensive clearing to keep it from reinvading infested areas after clearing as shown by the seed rain data. The high seedling density of lantana in the seed banks and in the cleared areas shows the need for ongoing follow-up in order to deplete soil stored seed banks. There is need for longer term research to establish what the exact follow-up requirements are in order to contain lantana re-infestation and create favourable micro-sites for native species to establish. It is predicted that ongoing lantana control in the cleared plots will most likely initiate long-term community recovery.
- Full Text:
- Date Issued: 2010
- Authors: Nang'alelwa, Michael Mubitelela
- Date: 2010
- Subjects: Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4217 , http://hdl.handle.net/10962/d1003786 , Lantana camara -- Victoria Falls (Zambia and Zimbabwe) , Lantana camara -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Invasive plants -- Biological control -- Victoria Falls (Zambia and Zimbabwe) , Riparian restoration -- Victoria Falls (Zambia and Zimbabwe) , Riparian ecology -- Victoria Falls (Zambia and Zimbabwe)
- Description: The exotic plant Lantana camara L. has invaded the riparian areas of the Victoria Falls World Heritage Site in Livingstone, southern Zambia, threatening native plant communities which support populations of species of special concern. I trialled the mechanical control method of manual uprooting and 3 different herbicides applied through paint brushing of an imazapyr concentrate at 250g. l¯¹, spraying on cut stumps with metsulfron methyl at 600g.l¯¹, and foliar spraying on re-emergent lantana foliage with glyphosate at a dosage of 166g. l¯¹ in July 2008 in 20 100m2 treatment plots, 5 invaded control plots and 5 uninvaded controls. Follow-up treatments for re-sprouting lantana stumps and emerging seedlings were undertaken in June 2009. I measured effectiveness of the methods using adult lantana mortality in June 2009 and lantana seedling density in the different treatment plots during the follow-up exercise. The cost of the various methods and human labour applied were compared across the four treatments at initial clear and at follow-up. All treatments recorded a high adult lantana mortality rate, though there were no significant differences in lantana adult mortality amongst the treatments. Overall, uprooting had the highest adult mortality, followed by imazapyr, metsulfron and lastly glyphosate. Germination of lantana seedlings after clearing was high for all treatments but with no significant differences occurring between the treatments. Both adult lantana mortality and seedling density were however significantly different from the control. With labour included, chemical costs were far higher relative to uprooting, though uprooting costs were the highest when it came to the follow-up because of the emerging seedlings and some resprouting stumps. The effects of mechanical and chemical treatments on vegetation composition in the cleared areas were also assessed in order to detect any non-target and medium term effects of treatments. Contrary to expectation, none of the chemicals showed any significant effects on vegetation composition in the short and medium-term and no significant differences were found in plant species richness, diversity and seedling density between invaded and uninvaded plots at baseline, in October 2008 and in September 2009. In order to determine potential for unaided vegetation recovery in the riparian areas of the study site after lantana clearing, I conducted an investigation of soil seed banks and seed rain using 60 seed bank samples measuring 1800m³ collected from 30 invaded and uninvaded plots. Using the seedling emergence method, 1, 991 seedlings belonging to 66 species representing 27 families germinated from the seed bank. Sedges (Cyperaceae family) were the most abundant taxa in the seed banks from invaded areas, followed by Ageratum conyzoides, lantana, Triumfetta annua and Achyranthes aspera which also occurred in the uninvaded soil seed banks. The seed banks from uninvaded plots were dominated by the grass Oplismenus hirtellus. Overall, species richness, diversity and seedling density from seed banks in invaded areas did not differ significantly from seed bank in uninvaded areas and there was a low similarity in species composition when above ground vegetation was compared to seed banks from invaded and uninvaded areas. It would appear if natural regeneration occured from the current seed bank in disturbed areas, future vegetation would largely comprise of short lived, early successional species in the short term as the seed bank is dominated by non-native herbaceous weedy species. From the seed traps investigating seed rain, a total of 27 species numbering 623 individual seeds were found in the thirty 1m² seedtraps distributed in invaded and uninvaded areas at the five sites, over an intermittent period of three months. Lantana had the highest monthly arrival rate in the seed traps followed by Phoenix reclinata and Ricinus communis. The number of species with invasive potential found in the seed traps located in invaded areas was more than that found in seed traps under native vegetation cover by far. Considerable forest remnants still occur around the invaded sites, and these could serve as an important source for long-term natural re-establishment of native vegetation if seed availability by animals and wind dispersal continues, while the re-invasion of lantana is prevented by ongoing follow-ups and futher clearing of lantana invaded areas. It is concluded that while uprooting and other treatments are effective in the control of lantana, its successful control in the Victoria Falls World Heritage Site will require extensive clearing to keep it from reinvading infested areas after clearing as shown by the seed rain data. The high seedling density of lantana in the seed banks and in the cleared areas shows the need for ongoing follow-up in order to deplete soil stored seed banks. There is need for longer term research to establish what the exact follow-up requirements are in order to contain lantana re-infestation and create favourable micro-sites for native species to establish. It is predicted that ongoing lantana control in the cleared plots will most likely initiate long-term community recovery.
- Full Text:
- Date Issued: 2010
Elevated CO2 determines cell damage and nitrogen allocation in barley subjected to aphid herbivory
- Authors: Gallagher, Sean
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64337 , vital:28535
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2018
- Authors: Gallagher, Sean
- Date: 2018
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64337 , vital:28535
- Description: Expected release date-May 2019
- Full Text:
- Date Issued: 2018
Environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the Karoo
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4794 , http://hdl.handle.net/10962/d1001894
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
- Date Issued: 1991
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4794 , http://hdl.handle.net/10962/d1001894
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
- Date Issued: 1991
Environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the Karoo
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4175 , http://hdl.handle.net/10962/d1002023
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
- Date Issued: 1991
- Authors: Hobson, Colin Desmond
- Date: 1991
- Subjects: Botany, Economic Plant introduction -- South Africa -- Karoo Plants, Cultivated
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: vital:4175 , http://hdl.handle.net/10962/d1002023
- Description: This study examines the environmental and socio-economic effects associated with the planting of Atriplex nummularia Lindl. (Oldman saltbush) in the karoo. This is the first study of its kind on oldman saltbush and serves as an important scoping exercise in assessing the impact of saltbush on its surrounds and in identifying additional aspects which require investigation. The environmental parameters investigated include microclimatic effects, soil induced changes, salinity effects on the germination of selected karoo species and compositional changes in the indigenous plant community within a saltbush plantation. When compared to indigenous karoo plant species oldman saltbush is found to ameliorate soil temperatures and increase surface soil salinity (specifically sodium chloride), on an individual plant basis. Salinity is shown to have a differential effect on the germination of selected karoo shrubs. The indigenous plant community within a forty year old saltbush plantation is impoverished with both density of individuals and species richness being markedly reduced. Saltbush is identified as an unsuitable nurse plant for the majority of karoo shrubs. Farm size in relation to both actual and intended area established to saltbush is considered. The area of saltbush already established in the karoo is considered too insignificant to have any wide socio-economic impact. Farmers who intend planting large areas of their farms to saltbush are in the minority. It is those farmers who already have some saltbush planted, who intend planting more. Ecologically based management guidelines are presented, suggesting practices which minimize the detrimental effects of saltbush on soil salinity and the indigenous plant community. These guidelines are practical and in keeping with the overall objectives of optimum resource utilisation and the national grazing strategy for South Africa.
- Full Text:
- Date Issued: 1991
Evaluating the physiological, morphological and nutritional effects of elevated atmospheric CO2 and drought on select South African maize cultivars
- Authors: Bopape, Tebadi Mamadiga
- Date: 2021-10
- Subjects: Corn Varieties South Africa , Dry farming South Africa , Corn Effect of atmospheric carbon dioxide on South Africa , Corn Effect of drought on South Africa , Corn Morphology , Corn Nutrition , Corn Physiological effect
- Language: English
- Type: Master theses , text
- Identifier: http://hdl.handle.net/10962/189020 , vital:44807
- Description: Thesis embargoed. Expected Release date October 2022. , Thesis (MSc) -- Faculty of Science, Botany, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Bopape, Tebadi Mamadiga
- Date: 2021-10
- Subjects: Corn Varieties South Africa , Dry farming South Africa , Corn Effect of atmospheric carbon dioxide on South Africa , Corn Effect of drought on South Africa , Corn Morphology , Corn Nutrition , Corn Physiological effect
- Language: English
- Type: Master theses , text
- Identifier: http://hdl.handle.net/10962/189020 , vital:44807
- Description: Thesis embargoed. Expected Release date October 2022. , Thesis (MSc) -- Faculty of Science, Botany, 2021
- Full Text:
- Date Issued: 2021-10
Evolutionary and biogeographic studies in the genus Kniphofia moench (Asphodelaceae)
- Authors: Ramdhani, Syd
- Date: 2007
- Subjects: Asphodelaceae Asphodelaceae -- Genetics Cladistic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4220 , http://hdl.handle.net/10962/d1003789
- Description: Kniphofia, a genus of approximately 71 species, is almost entirely African with two species occurring in Madagascar and one in Yemen. Commonly known as ‘red hot pokers’ they are popular among horticulturists. The genus is also well known for its complex alpha taxonomy. To date, no studies have examined the phylogenetic relationships among species or the evolutionary history of the genus, and little work has been done on their biogeography. The main focus of this study was (i) to review the alpha taxonomy, (ii) to assess diversity and endemism in Kniphofia, (iii) to use DNA sequence data to reconstruct a specieslevel phylogeny to understand intra-generic species relationships and evolutionary processes (iv) to use phylogeographic approaches to study the biogeography and evaluate biogeographical patterns, and (v) to assess anatomical variation and determine if anatomical characters are useful for species delimitation. It was found that the genus has six centres of diversity, five of which are centres of endemism. The South African Centre is the most speciose and is also the largest centre of endemism. Kniphofia shows a strong Afromontane grassland affinity in Tropical and East Africa. In South Africa, it is found from high altitudes to coastal habitats, with the most speciose regions being Afromontane grasslands. It is thus not considered to be an Afromontane element, but rather an Afromontane associate. Five major evolutionary lineages were identified using cpDNA sequence data (trnT-L spacer), four of which are southern African. The fifth lineage is represented by material from Madagascar, East and Tropical Africa. The nuclear ITS region failed to provide resolution, as many sequences were identical. The five lineages recovered using cpDNA showed some congruence with geographic origin rather than the taxonomic arrangement based on morphology. All of the species with multiple samples were non-monophyletic. This could be due to hybridisation and/or incomplete lineage sorting. The nested clade analysis, although preliminary, did not completely agree with the phylogenetic analyses. One of the three third level nested clades appears to show fragmentation between the Cape Region, KwaZulu-Natal and northern parts of southern Africa. Furthermore, another nested clades recovered suggest a range expansion and radiation from the Drakensberg into the adjacent Drakensberg-Maputoland-Pondoland transition. Morphological species of Kniphofia exhibited substantial leaf anatomical variation and anatomical characters do not cluster samples into their morphological species. The anatomical results do not fit any geographic pattern, nor do they correspond to the lineages recovered using molecular markers or the nested clades. Leaf anatomical variation does not appear to be influenced by geographical or environmental factors. However, hybridisation may play a role but was not tested in this study. In light of the above findings it is proposed that the evolutionary and biogeographic history of Kniphofia is strongly linked to tectonic events, and Quaternary climatic cycles and vegetation changes. Tectonic events (viz. uplifts) may have resulted in vicariance events that may account for the five cpDNA lineages recovered in phylogenetic analyses, while Quaternary climatic cycles and vegetation changes may have had a more recent impact on evolution and biogeography. It is hypothesised that the ancestral area for Kniphofia was much more widespread when Afromontane grasslands were more extensive during cooler and drier glacial episodes. Kniphofia on the high mountains of Tropical and East Africa would have tracked Afromontane grasslands as they expanded their ranges in cooler periods. While during wetter and warmer interglacial periods Kniphofia would have retreated into refugia on the mountains of Tropical and East Africa, with no gene flow possible between these refugia. In South Africa, where latitude compensates for altitude, Kniphofia may have maintained a distribution that extended into the lowlands even during interglacials. A cyclic climate change hypothesis implies that populations of Kniphofia (at different phases of the climatic cycle) would have experienced periods of contractions and fragmentation followed by periods of range expansion and coalescence or secondary contact. Altitudinal shifting is proposed to be the most likely mechanism for fragmentation and range expansion, and would would possibly promoted hybridisation. Within the five lineages there is evidence for recent differentiation as the branch lengths are short, there are numerous nonmonophyletic species and numerous identical haplotypes (cpDNA and ITS) which collectively indicate a recent radiation in southern Africa. A recent radiation would also account for the taxonomic confusion and difficulty in differentiating morpho-species. These climatic events may also account for the substantial anatomical variation in southern African Kniphofia species.
- Full Text:
- Date Issued: 2007
- Authors: Ramdhani, Syd
- Date: 2007
- Subjects: Asphodelaceae Asphodelaceae -- Genetics Cladistic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4220 , http://hdl.handle.net/10962/d1003789
- Description: Kniphofia, a genus of approximately 71 species, is almost entirely African with two species occurring in Madagascar and one in Yemen. Commonly known as ‘red hot pokers’ they are popular among horticulturists. The genus is also well known for its complex alpha taxonomy. To date, no studies have examined the phylogenetic relationships among species or the evolutionary history of the genus, and little work has been done on their biogeography. The main focus of this study was (i) to review the alpha taxonomy, (ii) to assess diversity and endemism in Kniphofia, (iii) to use DNA sequence data to reconstruct a specieslevel phylogeny to understand intra-generic species relationships and evolutionary processes (iv) to use phylogeographic approaches to study the biogeography and evaluate biogeographical patterns, and (v) to assess anatomical variation and determine if anatomical characters are useful for species delimitation. It was found that the genus has six centres of diversity, five of which are centres of endemism. The South African Centre is the most speciose and is also the largest centre of endemism. Kniphofia shows a strong Afromontane grassland affinity in Tropical and East Africa. In South Africa, it is found from high altitudes to coastal habitats, with the most speciose regions being Afromontane grasslands. It is thus not considered to be an Afromontane element, but rather an Afromontane associate. Five major evolutionary lineages were identified using cpDNA sequence data (trnT-L spacer), four of which are southern African. The fifth lineage is represented by material from Madagascar, East and Tropical Africa. The nuclear ITS region failed to provide resolution, as many sequences were identical. The five lineages recovered using cpDNA showed some congruence with geographic origin rather than the taxonomic arrangement based on morphology. All of the species with multiple samples were non-monophyletic. This could be due to hybridisation and/or incomplete lineage sorting. The nested clade analysis, although preliminary, did not completely agree with the phylogenetic analyses. One of the three third level nested clades appears to show fragmentation between the Cape Region, KwaZulu-Natal and northern parts of southern Africa. Furthermore, another nested clades recovered suggest a range expansion and radiation from the Drakensberg into the adjacent Drakensberg-Maputoland-Pondoland transition. Morphological species of Kniphofia exhibited substantial leaf anatomical variation and anatomical characters do not cluster samples into their morphological species. The anatomical results do not fit any geographic pattern, nor do they correspond to the lineages recovered using molecular markers or the nested clades. Leaf anatomical variation does not appear to be influenced by geographical or environmental factors. However, hybridisation may play a role but was not tested in this study. In light of the above findings it is proposed that the evolutionary and biogeographic history of Kniphofia is strongly linked to tectonic events, and Quaternary climatic cycles and vegetation changes. Tectonic events (viz. uplifts) may have resulted in vicariance events that may account for the five cpDNA lineages recovered in phylogenetic analyses, while Quaternary climatic cycles and vegetation changes may have had a more recent impact on evolution and biogeography. It is hypothesised that the ancestral area for Kniphofia was much more widespread when Afromontane grasslands were more extensive during cooler and drier glacial episodes. Kniphofia on the high mountains of Tropical and East Africa would have tracked Afromontane grasslands as they expanded their ranges in cooler periods. While during wetter and warmer interglacial periods Kniphofia would have retreated into refugia on the mountains of Tropical and East Africa, with no gene flow possible between these refugia. In South Africa, where latitude compensates for altitude, Kniphofia may have maintained a distribution that extended into the lowlands even during interglacials. A cyclic climate change hypothesis implies that populations of Kniphofia (at different phases of the climatic cycle) would have experienced periods of contractions and fragmentation followed by periods of range expansion and coalescence or secondary contact. Altitudinal shifting is proposed to be the most likely mechanism for fragmentation and range expansion, and would would possibly promoted hybridisation. Within the five lineages there is evidence for recent differentiation as the branch lengths are short, there are numerous nonmonophyletic species and numerous identical haplotypes (cpDNA and ITS) which collectively indicate a recent radiation in southern Africa. A recent radiation would also account for the taxonomic confusion and difficulty in differentiating morpho-species. These climatic events may also account for the substantial anatomical variation in southern African Kniphofia species.
- Full Text:
- Date Issued: 2007
Factors influencing ecological sustainability in the ostrich industry in the Little Karoo, South Africa
- Authors: Wheeler, Anita
- Date: 2014
- Subjects: Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4259 , http://hdl.handle.net/10962/d1011741 , Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Description: The Little Karoo, situated in the Succulent Karoo biome of South Africa, has been heavily transformed by land use, and only 8.6% of the remaining natural vegetation is considered to be intact. There is ample evidence that the main cause of degradation is the overstocking of ostriches, an industry that has been the major economic driver of the area for more than 150 years. The ostrich stocking rate currently recommended is 22 ha.ostrich⁻¹. A literature review was used to examine the evidence, assumptions and rationale on which recommended and actually implemented stocking rates for ostriches are based. No experimental evidence using ostriches was found that supported the recommended stocking rate as either ecologically or economically sustainable. From the literature, there appears to be a wide gap between what are considered to be economically and ecologically sustainable stocking rates, with the implication that these two aims may be impossible to reconcile when practicing ostrich farming on natural veld. A comparison of recommended with actual stocking rates among land managers in the Little Karoo showed that all land managers far exceeded the recommended agricultural stocking rate of 22.8 ha.ostrich⁻¹. However, the stocking rates reported by land managers to the South African Ostrich Business Chamber were found to accurately reflect actual numbers determined when whole flocks had to be slaughtered after an outbreak of avian influenza. The recommended stocking rate appeared to be irrelevant to ostrich flock breeders and there appeared to be a trade-off between profitable ostrich farming and sustainable land-use practices. This study also investigated the attitudes and behaviour of ostrich farmers. It was found that environmental attitude is most likely the most important characteristic of a land manager to ensure positive conservation behaviour. This characteristic was most prominent in younger land managers with larger farms. The last component of the study integrated the context and complexity of the long term social, economic and ecological sustainability of this industry through the development of a logic model. The results showed a general lack of linkages between industry elements which impact on achieving sustainability targets. Greater collaboration between industry role-players, organized agriculture and conservation organizations is required to find a balance between utilization and conservation in the ostrich industry.
- Full Text:
- Date Issued: 2014
- Authors: Wheeler, Anita
- Date: 2014
- Subjects: Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4259 , http://hdl.handle.net/10962/d1011741 , Ostrich farms -- South Africa -- Little Karoo , Land degradation -- South Africa -- Little Karoo , Land use -- South Africa -- Little Karoo , Land degradation -- Environmental aspects -- South Africa -- Little Karoo , Conservation of natural resources -- South Africa -- Little Karoo , Sustainable development -- South Africa -- Little Karoo , Sustainable agriculture -- South Africa -- Little Karoo
- Description: The Little Karoo, situated in the Succulent Karoo biome of South Africa, has been heavily transformed by land use, and only 8.6% of the remaining natural vegetation is considered to be intact. There is ample evidence that the main cause of degradation is the overstocking of ostriches, an industry that has been the major economic driver of the area for more than 150 years. The ostrich stocking rate currently recommended is 22 ha.ostrich⁻¹. A literature review was used to examine the evidence, assumptions and rationale on which recommended and actually implemented stocking rates for ostriches are based. No experimental evidence using ostriches was found that supported the recommended stocking rate as either ecologically or economically sustainable. From the literature, there appears to be a wide gap between what are considered to be economically and ecologically sustainable stocking rates, with the implication that these two aims may be impossible to reconcile when practicing ostrich farming on natural veld. A comparison of recommended with actual stocking rates among land managers in the Little Karoo showed that all land managers far exceeded the recommended agricultural stocking rate of 22.8 ha.ostrich⁻¹. However, the stocking rates reported by land managers to the South African Ostrich Business Chamber were found to accurately reflect actual numbers determined when whole flocks had to be slaughtered after an outbreak of avian influenza. The recommended stocking rate appeared to be irrelevant to ostrich flock breeders and there appeared to be a trade-off between profitable ostrich farming and sustainable land-use practices. This study also investigated the attitudes and behaviour of ostrich farmers. It was found that environmental attitude is most likely the most important characteristic of a land manager to ensure positive conservation behaviour. This characteristic was most prominent in younger land managers with larger farms. The last component of the study integrated the context and complexity of the long term social, economic and ecological sustainability of this industry through the development of a logic model. The results showed a general lack of linkages between industry elements which impact on achieving sustainability targets. Greater collaboration between industry role-players, organized agriculture and conservation organizations is required to find a balance between utilization and conservation in the ostrich industry.
- Full Text:
- Date Issued: 2014
Genetic studies on collagenolytic achromobacter strains and their bacteriophages
- Authors: Thomson, Jennifer Ann
- Date: 1974
- Subjects: Bacteriophages -- Genetics Bacterial genetics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4246 , http://hdl.handle.net/10962/d1007291
- Description: From Summary: A survey of collagenolytic aerobic bacteria from cured hides yielded three strains of Bacillus and eight of Achromobacter which degraded collagen at 0.4 M NaCl. Achromobacter sp. 2 was chosen for genetic studies due to its high collagenolytic activity and the lack of genetic information on Achromobacter. Four temperate bacteriophages specific for Achromobacter sp. 2 were isolated and their relationships studied. The phages caused lysogenic conversion resulting in the inability of lysogens to adsorb phage. Achromobacter sp. 2 was shown to be a cryptic lysogen as it was not immune to superinfection but had a very low rate of spontaneous induction which could be increased with mutagens. It is proposed that the cryptic lysogeny of this strain is maintained by a defective excision mechanism and the mode of prophage integration in the host chromosome. DNA extracted from phage α3a was used to transfect spheroplasts. The optimal conditions for the development of competence for transfection were determined. The presence of nuclease-attack on phage DNA under conditions of prolonged incubation of DNA and spheroplasts was proposed. A method for extracting Achromobacter DNA was devised which yielded purified, undegraded DNA, but it was not possible to transform Achromobacter sp. 2 with this DNA. The a phages were used to transduce a number of genetic markers into Achromobacter auxotrophs. The transduct ants had the ability to release the cryptic α3 prophage at a high rate while maintaining their sensitivity to homologous phage infection. It is proposed that this is due to complementation between the cryptic prophage and the residual phage functions in the transducing particles. The transductants segregated auxotrophs with a probability of 10⁻³ per cell per generation. It appears that an unusual system of generalised transduction is operating whereby the transducing particles contain both phage and bacterial DNA which is incorporated into the recipient genome by a single recombination event yielding unstable transductants. In a study on induction of Escherichia coli (λ), carcinogenic nitrosamines were shown to be inducers of phage development. This provides a screening system for potentially harmful nitrosamines.
- Full Text:
- Date Issued: 1974
- Authors: Thomson, Jennifer Ann
- Date: 1974
- Subjects: Bacteriophages -- Genetics Bacterial genetics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4246 , http://hdl.handle.net/10962/d1007291
- Description: From Summary: A survey of collagenolytic aerobic bacteria from cured hides yielded three strains of Bacillus and eight of Achromobacter which degraded collagen at 0.4 M NaCl. Achromobacter sp. 2 was chosen for genetic studies due to its high collagenolytic activity and the lack of genetic information on Achromobacter. Four temperate bacteriophages specific for Achromobacter sp. 2 were isolated and their relationships studied. The phages caused lysogenic conversion resulting in the inability of lysogens to adsorb phage. Achromobacter sp. 2 was shown to be a cryptic lysogen as it was not immune to superinfection but had a very low rate of spontaneous induction which could be increased with mutagens. It is proposed that the cryptic lysogeny of this strain is maintained by a defective excision mechanism and the mode of prophage integration in the host chromosome. DNA extracted from phage α3a was used to transfect spheroplasts. The optimal conditions for the development of competence for transfection were determined. The presence of nuclease-attack on phage DNA under conditions of prolonged incubation of DNA and spheroplasts was proposed. A method for extracting Achromobacter DNA was devised which yielded purified, undegraded DNA, but it was not possible to transform Achromobacter sp. 2 with this DNA. The a phages were used to transduce a number of genetic markers into Achromobacter auxotrophs. The transduct ants had the ability to release the cryptic α3 prophage at a high rate while maintaining their sensitivity to homologous phage infection. It is proposed that this is due to complementation between the cryptic prophage and the residual phage functions in the transducing particles. The transductants segregated auxotrophs with a probability of 10⁻³ per cell per generation. It appears that an unusual system of generalised transduction is operating whereby the transducing particles contain both phage and bacterial DNA which is incorporated into the recipient genome by a single recombination event yielding unstable transductants. In a study on induction of Escherichia coli (λ), carcinogenic nitrosamines were shown to be inducers of phage development. This provides a screening system for potentially harmful nitrosamines.
- Full Text:
- Date Issued: 1974
Grazing sequence pattern and species selection by cattle in the Dohne sourveld
- Authors: Daines, Thomas
- Date: 1976
- Subjects: Grasses -- Research -- South Africa -- Cape of Good Hope , Grasses -- South Africa -- Cape of Good Hope -- Identification , Grazing -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4257 , http://hdl.handle.net/10962/d1010955
- Description: A detailed investigation into the harvesting of the standing grass crop within camps by cattle showed the following: 1. Cattle have a definite order of preference for the components of the Dohne Sourveld grass sward. The most palatable species in order of preference (based on Corrected Species Importance rating) are Themeda triandra, Tristachya hispida, Andropogon appendiculatus, Alloteropsis semialata and Heteropogon contortus. The least preferred species is Elionurus argenteus. 2. The key species, which is defined as the most abundant and productive palatable species, has been identified as Themeda triandra. 3. In harvesting the grass crop, cattle not only have a set preference for species, but also have a set grazing sequence pattern. This can be divided into three main phases. On entering the camp the animals first select the species of their choice, "creaming" these plants (grazed less than 50%) to obtain the most nutritious feed. In their daily search for food they spread their grazing over an increasingly wider area and over a wider range of species. When they can no longer obtain their daily requirements from this "creaming" operation of the more preferred species they return to the most preferred species , which are then heavily grazed (more than 50%) producing the common pattern of area grazing within a camp. These areas increase in size as the cattle graze down the species of their choice, moving to less preferred species as soon as they can no longer obtain their daily intake requirements from the more preferred species. Finally when they have no alternative they graze the least preferred species. 4. Increasing the stocking density does not alter the grazing sequence pattern or change the order of preference for the component species in the sward. It only increases the rate of utilization of the more preferred species and the various phases in the grazing sequence pattern are reached more rapidly. 5. At higher stocking densities the palatable species are more evenly utilized than at lower stocking densities. 6. Cattle graze a greater variety of species when the sward is young and actively growing. As the maturity of the sward increases the cattle concentrate on the more preferred species and are more selective in their grazing. However, their order of preference remains the same. Species that are highly preferred early in the season retain their ranking when the sward is mature. The only exception to this rule is Alloteropsis semialata. Early in the growing season this species is high on the preference list, but as it matures less is taken until when fully mature, it is rejected by cattle. 7. Elionurus argenteus is the least preferred of all the species in the Dohne Sourveld. Cattle will graze this plant as a last resort when grazing a camp early in the season, but reject the plant when it is fully mature. 8. To retain vigour and production of the Dohne Sourveld results of this investigation support the view that grazing management should be based on the principle of controlled selective grazing. Having shown that the more preferred species are over-utilized when attempting to utilize the least preferred species any grazing management that calls for total utilization of all standing vegetation should be rejected. 9. The results of this investigation supports a multicamp system for the Dohne Sourveld where high stocking densities are employed. This will result in the rapid and even utilization of the more preferred species. Management should be based on short period of stay and long period of absence. Cattle should be moved as soon as the key species have been well utilized, but not overgrazed. Cattle should only be returned to the camp when key species have made adequate regrowth following grazing. 10. A method, that is simple to use, is proposed for determining utilization of the veld and key species at different times of the year using the relationship between percentage of ungrazed plants and utilization of the sward and of Themeda triandra, the key grazing species in the sward. 11. Results show that when rested camps are grazed after May, the number of Themeda triandra inflorescences are drastically reduced. Veld that is specifically rested for the production of Themeda triandra seed should be rested from March of the year preceding expected seeding.
- Full Text:
- Date Issued: 1976
- Authors: Daines, Thomas
- Date: 1976
- Subjects: Grasses -- Research -- South Africa -- Cape of Good Hope , Grasses -- South Africa -- Cape of Good Hope -- Identification , Grazing -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4257 , http://hdl.handle.net/10962/d1010955
- Description: A detailed investigation into the harvesting of the standing grass crop within camps by cattle showed the following: 1. Cattle have a definite order of preference for the components of the Dohne Sourveld grass sward. The most palatable species in order of preference (based on Corrected Species Importance rating) are Themeda triandra, Tristachya hispida, Andropogon appendiculatus, Alloteropsis semialata and Heteropogon contortus. The least preferred species is Elionurus argenteus. 2. The key species, which is defined as the most abundant and productive palatable species, has been identified as Themeda triandra. 3. In harvesting the grass crop, cattle not only have a set preference for species, but also have a set grazing sequence pattern. This can be divided into three main phases. On entering the camp the animals first select the species of their choice, "creaming" these plants (grazed less than 50%) to obtain the most nutritious feed. In their daily search for food they spread their grazing over an increasingly wider area and over a wider range of species. When they can no longer obtain their daily requirements from this "creaming" operation of the more preferred species they return to the most preferred species , which are then heavily grazed (more than 50%) producing the common pattern of area grazing within a camp. These areas increase in size as the cattle graze down the species of their choice, moving to less preferred species as soon as they can no longer obtain their daily intake requirements from the more preferred species. Finally when they have no alternative they graze the least preferred species. 4. Increasing the stocking density does not alter the grazing sequence pattern or change the order of preference for the component species in the sward. It only increases the rate of utilization of the more preferred species and the various phases in the grazing sequence pattern are reached more rapidly. 5. At higher stocking densities the palatable species are more evenly utilized than at lower stocking densities. 6. Cattle graze a greater variety of species when the sward is young and actively growing. As the maturity of the sward increases the cattle concentrate on the more preferred species and are more selective in their grazing. However, their order of preference remains the same. Species that are highly preferred early in the season retain their ranking when the sward is mature. The only exception to this rule is Alloteropsis semialata. Early in the growing season this species is high on the preference list, but as it matures less is taken until when fully mature, it is rejected by cattle. 7. Elionurus argenteus is the least preferred of all the species in the Dohne Sourveld. Cattle will graze this plant as a last resort when grazing a camp early in the season, but reject the plant when it is fully mature. 8. To retain vigour and production of the Dohne Sourveld results of this investigation support the view that grazing management should be based on the principle of controlled selective grazing. Having shown that the more preferred species are over-utilized when attempting to utilize the least preferred species any grazing management that calls for total utilization of all standing vegetation should be rejected. 9. The results of this investigation supports a multicamp system for the Dohne Sourveld where high stocking densities are employed. This will result in the rapid and even utilization of the more preferred species. Management should be based on short period of stay and long period of absence. Cattle should be moved as soon as the key species have been well utilized, but not overgrazed. Cattle should only be returned to the camp when key species have made adequate regrowth following grazing. 10. A method, that is simple to use, is proposed for determining utilization of the veld and key species at different times of the year using the relationship between percentage of ungrazed plants and utilization of the sward and of Themeda triandra, the key grazing species in the sward. 11. Results show that when rested camps are grazed after May, the number of Themeda triandra inflorescences are drastically reduced. Veld that is specifically rested for the production of Themeda triandra seed should be rested from March of the year preceding expected seeding.
- Full Text:
- Date Issued: 1976
Growth and photosynthetic responses of Acacia (Vachellia) seedlings to atmospheric CO2 increased from glacial to current concentrations: underlying mechanisms and ecological implications
- Authors: Anderson, Bruce Maurice
- Date: 2020
- Subjects: Acacia vachellia , Acacia vachellia -- Growth , Acacia -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/138182 , vital:37604
- Description: The African Acacia species Vachellia karroo, V. robusta, V. nilotica, and V. tortilis are some of the most invasive species implicated in bush encroachment and woody thickening of historically open savannas in southern Africa. This is partially explained by historic increases in atmospheric CO2 concentrations, which are proposed to have promoted the growth and survivorship of C3 tree seedlings relative to C4 grasses. However, the uniformity of CO2 responsiveness and differences among Vachellia species remain largely undetermined. Here we investigate the growth and photosynthetic responses of four Vachellia species, all implicated in woody encroachment, but originating from distinct climatic niches. Exposing these species to a range of sub-ambient CO2 concentrations (12 – 40 Pa) showed that V. karroo, V. robusta, V. nilotica and V. tortilis all responded strongly and fairly consistently to increasing CO2 concentrations, acting as a ‘functional type’ despite being selected from different geographic regions and having different climatic niches. Combined average net CO2 assimilation rates increased by 130% despite significant, but low levels of down-regulation and decreased stomatal conductance. The increased photosynthetic rates stimulated growth and biomass production in all compartments, with no significant differences in interspecific above and below ground allocation. Growth rates and dry biomass increased by 50% and 186%, respectively, while leaf level water use efficiency (ratio of net CO2 assimilation rate to transpiration rate) increased by an average of 218%. When this was scaled to the whole plant level, this stimulation was decreased to 80%. The decrease was the result of the CO2 stimulated increase in canopy areas, which increased leaf area for water loss. The seedlings’ total number of spinescent physical defenses, as well as the average mass and spine mass fraction also increased with rising CO2. These thicker spines could act as better deterrents against vertebrate browsers. Spine density was unchanged, however, showing that the increased spine numbers were associated with larger seedlings at higher CO2 rather than an increase in the number of spines per stem length. The stimulatory effects of increasing CO2 concentrations since the last glacial maximum and resultant increases in seedling growth and biomass are likely to have had important consequences for the survival and establishment of Acacia seedlings. Tolerance of drought and disturbance has been related to seedling size, hence stimulating the growth rate could confer disturbance tolerance and this tolerance would develop more rapidly with increasing CO2 concentrations. Furthermore, increased nitrogen and water use efficiency have the potential to support seedling establishment in environments where these resources would otherwise be limited at lower atmospheric CO2 concentrations. Resulting in a larger proportion of CO2 fertilization responsive woody seedlings surviving the seedling size classes, and persisting within historically open savannas. Where interspecific differences occurred they are likely to have arisen from adaptation to specific climates where these species are native and selection would have been driven by factors such climate, resource availability, levels of disturbance and competitive interactions. V. karroo had the highest growth rates and strong CO2 driven increases in biomass accumulation, despite having the lowest inherent photosynthetic rates. V. karroo also had the lowest increase in water use efficiency and high transpiration rates could potentially increase access to soil nutrients through mass flow. This species had the highest mean spine mass and showed significant increases in spine mass fraction at elevated CO2 concentrations, which may be important for deterring herbivores. V. robusta’s distribution to the mesic east coast of Africa suggests that water is an important limitation to its distribution. Hence, the CO2 stimulated increase in water use efficiency at both leaf and whole canopy level allows speculation that this may be an important driver of this species’ range expansion, which might continue if increasing levels of CO2 continue to promote water use efficiency. V. nilotica occupies a broad range of habitats, inhabiting large areas of the subtropics both north and south of the equator, with the strongest climatic correlates being the precipitation of the wettest quarter followed by high temperature seasonality. In response to increasing CO2, V. nilotica showed overall strong increases in growth, water use efficiency, and physical defenses. These responses may explain why V. nilotica has been such a successful encroacher in a broad range of habitats where limitations are likely to include multiple climatic factors and disturbances. V. tortilis has the widest distribution of all the species studied, covering broad ranges of Africa and only being excluded from the wettest parts of the equator and driest parts of the deserts. In these experiments this species showed the lowest biomass responsiveness to CO2, but had especially large increases in water use efficiency at both the leaf and canopy level. This may have been an important driver for this species’ encroachment into the more arid parts of its distribution, however this link will need to be verified with further experimentation.
- Full Text:
- Date Issued: 2020
- Authors: Anderson, Bruce Maurice
- Date: 2020
- Subjects: Acacia vachellia , Acacia vachellia -- Growth , Acacia -- South Africa
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/138182 , vital:37604
- Description: The African Acacia species Vachellia karroo, V. robusta, V. nilotica, and V. tortilis are some of the most invasive species implicated in bush encroachment and woody thickening of historically open savannas in southern Africa. This is partially explained by historic increases in atmospheric CO2 concentrations, which are proposed to have promoted the growth and survivorship of C3 tree seedlings relative to C4 grasses. However, the uniformity of CO2 responsiveness and differences among Vachellia species remain largely undetermined. Here we investigate the growth and photosynthetic responses of four Vachellia species, all implicated in woody encroachment, but originating from distinct climatic niches. Exposing these species to a range of sub-ambient CO2 concentrations (12 – 40 Pa) showed that V. karroo, V. robusta, V. nilotica and V. tortilis all responded strongly and fairly consistently to increasing CO2 concentrations, acting as a ‘functional type’ despite being selected from different geographic regions and having different climatic niches. Combined average net CO2 assimilation rates increased by 130% despite significant, but low levels of down-regulation and decreased stomatal conductance. The increased photosynthetic rates stimulated growth and biomass production in all compartments, with no significant differences in interspecific above and below ground allocation. Growth rates and dry biomass increased by 50% and 186%, respectively, while leaf level water use efficiency (ratio of net CO2 assimilation rate to transpiration rate) increased by an average of 218%. When this was scaled to the whole plant level, this stimulation was decreased to 80%. The decrease was the result of the CO2 stimulated increase in canopy areas, which increased leaf area for water loss. The seedlings’ total number of spinescent physical defenses, as well as the average mass and spine mass fraction also increased with rising CO2. These thicker spines could act as better deterrents against vertebrate browsers. Spine density was unchanged, however, showing that the increased spine numbers were associated with larger seedlings at higher CO2 rather than an increase in the number of spines per stem length. The stimulatory effects of increasing CO2 concentrations since the last glacial maximum and resultant increases in seedling growth and biomass are likely to have had important consequences for the survival and establishment of Acacia seedlings. Tolerance of drought and disturbance has been related to seedling size, hence stimulating the growth rate could confer disturbance tolerance and this tolerance would develop more rapidly with increasing CO2 concentrations. Furthermore, increased nitrogen and water use efficiency have the potential to support seedling establishment in environments where these resources would otherwise be limited at lower atmospheric CO2 concentrations. Resulting in a larger proportion of CO2 fertilization responsive woody seedlings surviving the seedling size classes, and persisting within historically open savannas. Where interspecific differences occurred they are likely to have arisen from adaptation to specific climates where these species are native and selection would have been driven by factors such climate, resource availability, levels of disturbance and competitive interactions. V. karroo had the highest growth rates and strong CO2 driven increases in biomass accumulation, despite having the lowest inherent photosynthetic rates. V. karroo also had the lowest increase in water use efficiency and high transpiration rates could potentially increase access to soil nutrients through mass flow. This species had the highest mean spine mass and showed significant increases in spine mass fraction at elevated CO2 concentrations, which may be important for deterring herbivores. V. robusta’s distribution to the mesic east coast of Africa suggests that water is an important limitation to its distribution. Hence, the CO2 stimulated increase in water use efficiency at both leaf and whole canopy level allows speculation that this may be an important driver of this species’ range expansion, which might continue if increasing levels of CO2 continue to promote water use efficiency. V. nilotica occupies a broad range of habitats, inhabiting large areas of the subtropics both north and south of the equator, with the strongest climatic correlates being the precipitation of the wettest quarter followed by high temperature seasonality. In response to increasing CO2, V. nilotica showed overall strong increases in growth, water use efficiency, and physical defenses. These responses may explain why V. nilotica has been such a successful encroacher in a broad range of habitats where limitations are likely to include multiple climatic factors and disturbances. V. tortilis has the widest distribution of all the species studied, covering broad ranges of Africa and only being excluded from the wettest parts of the equator and driest parts of the deserts. In these experiments this species showed the lowest biomass responsiveness to CO2, but had especially large increases in water use efficiency at both the leaf and canopy level. This may have been an important driver for this species’ encroachment into the more arid parts of its distribution, however this link will need to be verified with further experimentation.
- Full Text:
- Date Issued: 2020
Investigating the effect of Glomus etunicatum colonization on structure and phloem transport in roots of Eragrostis curvula (Umgeni)
- Authors: Skinner, Amy
- Date: 2007
- Subjects: Glomus (Fungi) , Phloem , Plant translocation , Weeping lovegrass , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Mycorrhizas
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4227 , http://hdl.handle.net/10962/d1003796 , Glomus (Fungi) , Phloem , Plant translocation , Weeping lovegrass , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Mycorrhizas
- Description: The symbiotic unit of an arbuscular mycorrhizal fungus and its host is able toachieve and maintain far higher inflow of nutrients than non-mycorrhizal roots. The colonization strategy of the mycobiont within the plant is intrinsic to the symbiosis with respect to both structural adaptations and nutrient exchange. An investigation into the effect of Glomus etunicatum colonization on the structure and phloem transport in Eragrostis curvula (Umgeni) allowed for greater insight into the dynamic of the symbiosis. The combined use of stains (such as Trypan Blue, Chlorazol Black, Safranin and Fast Green), and techniques, (such as freeze-microtome transverse sectioning and permanent slide preparations) contributed to a successful general observation of an intermediate colonization strategy using light microscopy methods. However, clarity into structural detail of mycorrhizal forms required electron microscopy studies. The SEM method used with freeze fracture was a relatively quick and simple method allowing for the observation of surface and internal features. The TEM method allowed for highresolution images providing insight into the variations in the apoplasmic compartmental form, and how this may relate to the function of the symbiosis with regard to fungal coils or arbuscules. The apoplasmic nature of mycorrhizas was substantiated and no symplasmic connections were found between symbionts. Fluorescence studies demonstrated that 5,6-carboxyfluorescein was transported through the phloem into the roots of E. curvula, but remained predominantly in the root phloem. Unloading only occurred in optimal nutrient exchange areas of meristimatic lateral or apical growth regions. It was not possible, using fluorescence techniques and related equipment available, to conclusively establish if there were symplasmic connections between the mycobiont and its host or if bidirectional transfer of nutrients occurred at the same interface.
- Full Text:
- Date Issued: 2007
- Authors: Skinner, Amy
- Date: 2007
- Subjects: Glomus (Fungi) , Phloem , Plant translocation , Weeping lovegrass , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Mycorrhizas
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4227 , http://hdl.handle.net/10962/d1003796 , Glomus (Fungi) , Phloem , Plant translocation , Weeping lovegrass , Vesicular-arbuscular mycorrhizas , Mycorrhizal fungi , Mycorrhizas
- Description: The symbiotic unit of an arbuscular mycorrhizal fungus and its host is able toachieve and maintain far higher inflow of nutrients than non-mycorrhizal roots. The colonization strategy of the mycobiont within the plant is intrinsic to the symbiosis with respect to both structural adaptations and nutrient exchange. An investigation into the effect of Glomus etunicatum colonization on the structure and phloem transport in Eragrostis curvula (Umgeni) allowed for greater insight into the dynamic of the symbiosis. The combined use of stains (such as Trypan Blue, Chlorazol Black, Safranin and Fast Green), and techniques, (such as freeze-microtome transverse sectioning and permanent slide preparations) contributed to a successful general observation of an intermediate colonization strategy using light microscopy methods. However, clarity into structural detail of mycorrhizal forms required electron microscopy studies. The SEM method used with freeze fracture was a relatively quick and simple method allowing for the observation of surface and internal features. The TEM method allowed for highresolution images providing insight into the variations in the apoplasmic compartmental form, and how this may relate to the function of the symbiosis with regard to fungal coils or arbuscules. The apoplasmic nature of mycorrhizas was substantiated and no symplasmic connections were found between symbionts. Fluorescence studies demonstrated that 5,6-carboxyfluorescein was transported through the phloem into the roots of E. curvula, but remained predominantly in the root phloem. Unloading only occurred in optimal nutrient exchange areas of meristimatic lateral or apical growth regions. It was not possible, using fluorescence techniques and related equipment available, to conclusively establish if there were symplasmic connections between the mycobiont and its host or if bidirectional transfer of nutrients occurred at the same interface.
- Full Text:
- Date Issued: 2007
Life history, population dynamics and conservation status of Oldenburgia grandis (Asteraceae), an endemic of the Eastern Cape of South Africa
- Authors: Swart, Carin
- Date: 2008
- Subjects: Compositae , Fynbos ecology -- South Africa -- Eastern Cape , Endemic plants -- South Africa -- Eastern Cape , Rare plants -- South Africa -- Eastern Cape , Plant populations -- South Africa -- Eastern Cape , Vegetation dynamics -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Endangered species -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4230 , http://hdl.handle.net/10962/d1003799 , Compositae , Fynbos ecology -- South Africa -- Eastern Cape , Endemic plants -- South Africa -- Eastern Cape , Rare plants -- South Africa -- Eastern Cape , Plant populations -- South Africa -- Eastern Cape , Vegetation dynamics -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Endangered species -- South Africa -- Eastern Cape
- Description: Oldenburgia grandis is a rare, long-lived woody paleoendemic of the Fynbos Biome of South Africa. Confined to quartzite outcrops, it has a small geographic range and narrow habitat specificity. O. grandis responds to its fire-prone environment by resprouting. Elasticity analysis of O. grandis reveals that growth and fecundity were traded off for persistence of adult, mature and sapling stages. Morphological adaptations such as a corky fire-resistant bark and the ability to resprout after fire are traits that O. grandis have evolved to persist in a frequently disturbed environment. Population growth rate for sites undisturbed by fire for a number of years (l = 1.01) and sites at various stages of recovery after fire (l = 1.00) were very similar. The highest variation in transition probabilities for all sites was seen in the persistence of the seedling stage and growth from seedling to sapling. Observed population structure and stable stage distribution determined by the matrix model show that sites recently undisturbed by fire had high abundances of the adult and sapling stages. A peak in sapling stages was seen for the stable stage distribution where similar peak in sapling numbers were seen for population structures of sites at various stages of recoveryafter fire. Favourable environmental conditions for the persistence of O. grandis populations include no fire with transition probabilities between the observed minimum and maximum and fire frequency at a 10 year interval where seedling protection from the fire is high and adult and mature mortalities during the fire are low. Stochastic environmental events that could put populations (particularly small populations) at an increased risk of extinction include high to moderate fire intensities where seedling protection from the fire is low and adult and mature mortalities are high as a result of the fire.
- Full Text:
- Date Issued: 2008
- Authors: Swart, Carin
- Date: 2008
- Subjects: Compositae , Fynbos ecology -- South Africa -- Eastern Cape , Endemic plants -- South Africa -- Eastern Cape , Rare plants -- South Africa -- Eastern Cape , Plant populations -- South Africa -- Eastern Cape , Vegetation dynamics -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Endangered species -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4230 , http://hdl.handle.net/10962/d1003799 , Compositae , Fynbos ecology -- South Africa -- Eastern Cape , Endemic plants -- South Africa -- Eastern Cape , Rare plants -- South Africa -- Eastern Cape , Plant populations -- South Africa -- Eastern Cape , Vegetation dynamics -- South Africa -- Eastern Cape , Plant conservation -- South Africa -- Eastern Cape , Endangered species -- South Africa -- Eastern Cape
- Description: Oldenburgia grandis is a rare, long-lived woody paleoendemic of the Fynbos Biome of South Africa. Confined to quartzite outcrops, it has a small geographic range and narrow habitat specificity. O. grandis responds to its fire-prone environment by resprouting. Elasticity analysis of O. grandis reveals that growth and fecundity were traded off for persistence of adult, mature and sapling stages. Morphological adaptations such as a corky fire-resistant bark and the ability to resprout after fire are traits that O. grandis have evolved to persist in a frequently disturbed environment. Population growth rate for sites undisturbed by fire for a number of years (l = 1.01) and sites at various stages of recovery after fire (l = 1.00) were very similar. The highest variation in transition probabilities for all sites was seen in the persistence of the seedling stage and growth from seedling to sapling. Observed population structure and stable stage distribution determined by the matrix model show that sites recently undisturbed by fire had high abundances of the adult and sapling stages. A peak in sapling stages was seen for the stable stage distribution where similar peak in sapling numbers were seen for population structures of sites at various stages of recoveryafter fire. Favourable environmental conditions for the persistence of O. grandis populations include no fire with transition probabilities between the observed minimum and maximum and fire frequency at a 10 year interval where seedling protection from the fire is high and adult and mature mortalities during the fire are low. Stochastic environmental events that could put populations (particularly small populations) at an increased risk of extinction include high to moderate fire intensities where seedling protection from the fire is low and adult and mature mortalities are high as a result of the fire.
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- Date Issued: 2008