Molecular characterization of the tetratricopeptide repeat-mediated interactions of murine stress-inducible protein 1 with major heat shock proteins
- Authors: Odunuga, Odutayo Odutola
- Date: 2003
- Subjects: Plants -- Effect of stress on Proteins -- Purification Electrophoresis Heat shock proteins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4091 , http://hdl.handle.net/10962/d1007724
- Description: Murine stress-inducible protein 1 (mSTI1) is a co-chaperone that is homologous with the human heat shock protein 70 (Hsp70)/heat shock protein 90 (Hsp90)-organizing protein (Hop). The two proteins are homologues of the highly conserved stress-inducible protein 1 (STI1) family of co-chaperones. The STI1 proteins interact directly and simultaneously at some stage, with Hsp70 and Hsp90 in the formation of the hetero-multi-chaperone complexes that facilitate the folding of signal transducing kinases and functional maturation of steroid hormone receptors. The interactions of mSTI1 with both Hsp70 and Hsp90 is mediated by a versatile structural protein-protein interaction motif, the tetratricopeptide repeat (TPR). The TPR motif is a degenerate 34-amino acid sequence a-helical structural motif found in a significant number of functionally unrelated proteins. This study was aimed at characterizing the structural and functional determinants in the TPR domains of mSTI1 responsible for binding to and discriminating between Hsp70 and Hsp90. Guided by data from Hop's crystal structures and amino acid sequence alignment analyses, various biochemical techniques were used to both qualitatively and quantitatively characterize the contacts necessary for the N-terminal TPR domain (TPR1) of mSTI1 to bind to the C-terminal EEVD motif of heat shock cognate protein 70 (Hsc70) and to discriminate between Hsc70 and Hsp90. Substitutions in the first TPR motif of Lys⁸ or Asn¹² did not affect binding of mSTI1 to Hsc70, while double substitution of these residues abrogated binding. A substitution in the second TPR motif of Asn⁴³ lowered but did not abrogate binding. Similarly, a deletion in the second TPR motif coupled with a substitution of Lys⁸ or Asn¹² reduced but did not abrogate binding. Steady state fluorescence and circular dichroism spectroscopies revealed that the double substitution of Lys⁸ and Asn¹² resulted in perturbations of inter-domain interactions in mSTl1. Together these results suggest that mSTI1-Hsc70 interaction requires a network of electrostatic interactions not only between charged residues in the TPR1 domain of mSTI1 and the EEVD motif of Hsc70, but also outside the TPR1 domain. It is proposed that the electrostatic interactions in the first TPR motif collectively made by Lys⁸ and Asn¹² define part of the minimum interactions required for successful mSTI1-Hsc70 interaction. In the first central TPR domain (TPR1A), single substitution of Lys³°¹ was sufficient to abrogate the mSTI1-Hsp90 interaction. Using a truncated derivative of mSTI1 incapable of binding to Hsp90, residues predicted by crystallographic data to determine Hsp70 binding specificity were substituted in the TPR1 domain. The modified protein had reduced binding to Hsc70, but showed significant binding capacity for Hsp90. In contrast, topologically equivalent substitutions on a truncated derivative of mSTI1 incapable of binding to Hsc70 did not confer Hsc70 specificity on the TPR2A domain. These data suggest that binding of Hsc70 to the TPR1 domain is more specific than binding of Hsp90 to the TPR2A domain. In addition, residues C-terminal of helix A in the second TPR motif of mSTI1 were shown to be important in determining specific binding to Hsc70. Binding assays using surface plasmon resonance spectroscopy showed that the affinities of binding of mSTI1 to Hsc70 and Hsp90 were 2 μM and 1.5 μM respectively. Preliminary in vivo studies revealed differences in the dynamics of binding of endogenous and exogenous recombinant mSTI1 with Hsc70 and Hsp90. The outcome of this study poses serious implications for the mechanisms of mSTI1 interactions with Hsc70 and Hsp90 in the cell.
- Full Text:
- Date Issued: 2003
- Authors: Odunuga, Odutayo Odutola
- Date: 2003
- Subjects: Plants -- Effect of stress on Proteins -- Purification Electrophoresis Heat shock proteins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4091 , http://hdl.handle.net/10962/d1007724
- Description: Murine stress-inducible protein 1 (mSTI1) is a co-chaperone that is homologous with the human heat shock protein 70 (Hsp70)/heat shock protein 90 (Hsp90)-organizing protein (Hop). The two proteins are homologues of the highly conserved stress-inducible protein 1 (STI1) family of co-chaperones. The STI1 proteins interact directly and simultaneously at some stage, with Hsp70 and Hsp90 in the formation of the hetero-multi-chaperone complexes that facilitate the folding of signal transducing kinases and functional maturation of steroid hormone receptors. The interactions of mSTI1 with both Hsp70 and Hsp90 is mediated by a versatile structural protein-protein interaction motif, the tetratricopeptide repeat (TPR). The TPR motif is a degenerate 34-amino acid sequence a-helical structural motif found in a significant number of functionally unrelated proteins. This study was aimed at characterizing the structural and functional determinants in the TPR domains of mSTI1 responsible for binding to and discriminating between Hsp70 and Hsp90. Guided by data from Hop's crystal structures and amino acid sequence alignment analyses, various biochemical techniques were used to both qualitatively and quantitatively characterize the contacts necessary for the N-terminal TPR domain (TPR1) of mSTI1 to bind to the C-terminal EEVD motif of heat shock cognate protein 70 (Hsc70) and to discriminate between Hsc70 and Hsp90. Substitutions in the first TPR motif of Lys⁸ or Asn¹² did not affect binding of mSTI1 to Hsc70, while double substitution of these residues abrogated binding. A substitution in the second TPR motif of Asn⁴³ lowered but did not abrogate binding. Similarly, a deletion in the second TPR motif coupled with a substitution of Lys⁸ or Asn¹² reduced but did not abrogate binding. Steady state fluorescence and circular dichroism spectroscopies revealed that the double substitution of Lys⁸ and Asn¹² resulted in perturbations of inter-domain interactions in mSTl1. Together these results suggest that mSTI1-Hsc70 interaction requires a network of electrostatic interactions not only between charged residues in the TPR1 domain of mSTI1 and the EEVD motif of Hsc70, but also outside the TPR1 domain. It is proposed that the electrostatic interactions in the first TPR motif collectively made by Lys⁸ and Asn¹² define part of the minimum interactions required for successful mSTI1-Hsc70 interaction. In the first central TPR domain (TPR1A), single substitution of Lys³°¹ was sufficient to abrogate the mSTI1-Hsp90 interaction. Using a truncated derivative of mSTI1 incapable of binding to Hsp90, residues predicted by crystallographic data to determine Hsp70 binding specificity were substituted in the TPR1 domain. The modified protein had reduced binding to Hsc70, but showed significant binding capacity for Hsp90. In contrast, topologically equivalent substitutions on a truncated derivative of mSTI1 incapable of binding to Hsc70 did not confer Hsc70 specificity on the TPR2A domain. These data suggest that binding of Hsc70 to the TPR1 domain is more specific than binding of Hsp90 to the TPR2A domain. In addition, residues C-terminal of helix A in the second TPR motif of mSTI1 were shown to be important in determining specific binding to Hsc70. Binding assays using surface plasmon resonance spectroscopy showed that the affinities of binding of mSTI1 to Hsc70 and Hsp90 were 2 μM and 1.5 μM respectively. Preliminary in vivo studies revealed differences in the dynamics of binding of endogenous and exogenous recombinant mSTI1 with Hsc70 and Hsp90. The outcome of this study poses serious implications for the mechanisms of mSTI1 interactions with Hsc70 and Hsp90 in the cell.
- Full Text:
- Date Issued: 2003
An exploration of market growth and development stategies for franchising a selected fast food outlet
- Authors: Snyman, Dion Cedric
- Date: 2003
- Subjects: Market share , Food industry and trade -- South Africa -- Management
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10896 , http://hdl.handle.net/10948/128 , Market share , Food industry and trade -- South Africa -- Management
- Description: The advent of globalisation and the re-admittance of South Africa into the international market place have presented both opportunities and threats to local fast food outlets and franchises. These food outlets are faced with foreign competition and the need to adapt their strategies in order to cope with the continued onslaught of these highly efficient, foreign, service orientated organisations if they intend to survive. This research explored the strategies these fast food outlets should utilise to deal with their competition and to facilitate their market growth and organisational development which could lead to them being successfully franchised at a later stage. The study revealed that if any significant growth and organisational development was to be expected, the fast food outlet had to concentrate on customer satisfaction and retention through the innovative use of the 7P’s of the marketing mix in its strategy formulation process. Attention also needed to be given to the competitive forces in the external environment so that competitive strategies were in line with market developments. The triangulation method was applied in this study. In terms of this method, qualitative and quantitative research techniques are combined in order to improve the reliability and validity of the research findings. This method proved to be an effective research tool as the findings in the quantitative research were verified by the findings in the qualitative research. Franchising in South Africa, especially the Eastern Cape, where unemployment is highest, is important as it has the potential for job creation and economic empowerment of the disadvantaged communities. It is therefore essential that every endeavour be made to ensure the success of the emerging entrepreneurs through assisting them to develop strategies that will ensure sustainable development and growth.
- Full Text:
- Date Issued: 2003
- Authors: Snyman, Dion Cedric
- Date: 2003
- Subjects: Market share , Food industry and trade -- South Africa -- Management
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10896 , http://hdl.handle.net/10948/128 , Market share , Food industry and trade -- South Africa -- Management
- Description: The advent of globalisation and the re-admittance of South Africa into the international market place have presented both opportunities and threats to local fast food outlets and franchises. These food outlets are faced with foreign competition and the need to adapt their strategies in order to cope with the continued onslaught of these highly efficient, foreign, service orientated organisations if they intend to survive. This research explored the strategies these fast food outlets should utilise to deal with their competition and to facilitate their market growth and organisational development which could lead to them being successfully franchised at a later stage. The study revealed that if any significant growth and organisational development was to be expected, the fast food outlet had to concentrate on customer satisfaction and retention through the innovative use of the 7P’s of the marketing mix in its strategy formulation process. Attention also needed to be given to the competitive forces in the external environment so that competitive strategies were in line with market developments. The triangulation method was applied in this study. In terms of this method, qualitative and quantitative research techniques are combined in order to improve the reliability and validity of the research findings. This method proved to be an effective research tool as the findings in the quantitative research were verified by the findings in the qualitative research. Franchising in South Africa, especially the Eastern Cape, where unemployment is highest, is important as it has the potential for job creation and economic empowerment of the disadvantaged communities. It is therefore essential that every endeavour be made to ensure the success of the emerging entrepreneurs through assisting them to develop strategies that will ensure sustainable development and growth.
- Full Text:
- Date Issued: 2003
Productivity and abundance of Sclerocarya birrea subsp. caffra in and around rural settlements and protected areas of the Bushbuckridge lowveld, South Africa
- Shackleton, Charlie M, Botha, Jenny, Emanuel, P L
- Authors: Shackleton, Charlie M , Botha, Jenny , Emanuel, P L
- Date: 2003
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/181607 , vital:43751 , xlink:href="https://doi.org/10.1080/14728028.2003.9752459"
- Description: Sclerocarya birrea (A. Rich.) Hochst, subsp. caffra (Sond.) Kokwaro is a common species throughout the semi-arid savannas of southern Africa, and is sought after by rural communities. It is frequently maintained in homesteads and fields in an agroforestry situation. It has also been identified by a number of international and national institutes as a key species for domestication and commercialisation. Yet, the sustainability of the resource and local user knowledge and practices have not been considered. This study reports on the abundance and productivity of S. birrea in four rural villages and neighbouring protected areas in the Bushbuckridge lowveld of the Limpopo Province, South Africa, where its common name is marula. The density of marula stems was measured in homestead plots, arable fields and the surrounding communal rangelands of each village. Fruit production was assessed by regular counting of fruit fall from marked trees of known size. Within the villages, fruit production was monitored via local community groups. Additional monitoring of fruiting was conducted on marked trees within local protected areas. The proportion of households planting or nurturing marula trees was determined through interviews with a stratified random sample of households. The density of marula trees was significantly higher in protected areas than the neighbouring communal lands, fields or homesteads. However, the majority of trees in protected areas were small, while those in homesteads were large and mature. Density of adult fruit-bearing female trees, was similar between the protected areas and homesteads, but less in fields and communal lands. S. birrea trees were maintained in 79 % of homesteads and 58 % of fields. Mean fruit yield per tree was significantly higher from village trees (less than 17 000 fruits) than protected area trees (more than 3 500 fruits), even after accounting for differences in tree size. The fresh mass of individual fruits was also greater on village trees than on trees in protected areas. Both these characteristics suggest some degree of historical selection of the trees in the homesteads and fields. There was a positive relationship between tree size and number of fruits produced.
- Full Text:
- Date Issued: 2003
- Authors: Shackleton, Charlie M , Botha, Jenny , Emanuel, P L
- Date: 2003
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/181607 , vital:43751 , xlink:href="https://doi.org/10.1080/14728028.2003.9752459"
- Description: Sclerocarya birrea (A. Rich.) Hochst, subsp. caffra (Sond.) Kokwaro is a common species throughout the semi-arid savannas of southern Africa, and is sought after by rural communities. It is frequently maintained in homesteads and fields in an agroforestry situation. It has also been identified by a number of international and national institutes as a key species for domestication and commercialisation. Yet, the sustainability of the resource and local user knowledge and practices have not been considered. This study reports on the abundance and productivity of S. birrea in four rural villages and neighbouring protected areas in the Bushbuckridge lowveld of the Limpopo Province, South Africa, where its common name is marula. The density of marula stems was measured in homestead plots, arable fields and the surrounding communal rangelands of each village. Fruit production was assessed by regular counting of fruit fall from marked trees of known size. Within the villages, fruit production was monitored via local community groups. Additional monitoring of fruiting was conducted on marked trees within local protected areas. The proportion of households planting or nurturing marula trees was determined through interviews with a stratified random sample of households. The density of marula trees was significantly higher in protected areas than the neighbouring communal lands, fields or homesteads. However, the majority of trees in protected areas were small, while those in homesteads were large and mature. Density of adult fruit-bearing female trees, was similar between the protected areas and homesteads, but less in fields and communal lands. S. birrea trees were maintained in 79 % of homesteads and 58 % of fields. Mean fruit yield per tree was significantly higher from village trees (less than 17 000 fruits) than protected area trees (more than 3 500 fruits), even after accounting for differences in tree size. The fresh mass of individual fruits was also greater on village trees than on trees in protected areas. Both these characteristics suggest some degree of historical selection of the trees in the homesteads and fields. There was a positive relationship between tree size and number of fruits produced.
- Full Text:
- Date Issued: 2003
Procedural fairness in unprotected strike dismissals
- Authors: Nel, Werner
- Date: 2003
- Subjects: Strikes and lockouts -- Law and legislation -- South Africa , Labor laws and legislation -- South Africa , Employees -- Dismissal of -- Law and legislation -- South Africa , South Africa. Labour Relations Act -- 1995
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11049 , http://hdl.handle.net/10948/314 , Strikes and lockouts -- Law and legislation -- South Africa , Labor laws and legislation -- South Africa , Employees -- Dismissal of -- Law and legislation -- South Africa , South Africa. Labour Relations Act -- 1995
- Description: The Labour Relations Act contains a definition of a strike which reads as follows: “’strike’ means the partial or complete concerted refusal to work, or the retardation or obstruction of work, by persons who are or have been employed by the same employer or by different employers, for the purpose of remedying a grievance or resolving a dispute in respect of any matter of mutual interest between employer and employee, and every reference to ‘work’ in this definition includes overtime work, whether it is voluntary or compulsory.” The Labour Relations Act offers strikers special protection against dismissal if they conform with the Act and its provisions. Hence the distinction between those strikes and protest action in compliance with the Act, namely ‘protected’ strikes and protest action, and those strikes and protest action in violation of the Act, namely, ‘unprotected’ strikes and protest action. Participation in an unprotected strike is one form of misbehaviour. The Labour Relations Act expressly prohibits the dismissal of employees engaged in a lawful strike. Employees engaged in strike action contrary to the provisions of the Labour Relations Act may be dismissed since their strike action is deemed to be a form of misconduct. The dismissal of striking employees must be both substantially and procedurally fair.
- Full Text:
- Date Issued: 2003
- Authors: Nel, Werner
- Date: 2003
- Subjects: Strikes and lockouts -- Law and legislation -- South Africa , Labor laws and legislation -- South Africa , Employees -- Dismissal of -- Law and legislation -- South Africa , South Africa. Labour Relations Act -- 1995
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11049 , http://hdl.handle.net/10948/314 , Strikes and lockouts -- Law and legislation -- South Africa , Labor laws and legislation -- South Africa , Employees -- Dismissal of -- Law and legislation -- South Africa , South Africa. Labour Relations Act -- 1995
- Description: The Labour Relations Act contains a definition of a strike which reads as follows: “’strike’ means the partial or complete concerted refusal to work, or the retardation or obstruction of work, by persons who are or have been employed by the same employer or by different employers, for the purpose of remedying a grievance or resolving a dispute in respect of any matter of mutual interest between employer and employee, and every reference to ‘work’ in this definition includes overtime work, whether it is voluntary or compulsory.” The Labour Relations Act offers strikers special protection against dismissal if they conform with the Act and its provisions. Hence the distinction between those strikes and protest action in compliance with the Act, namely ‘protected’ strikes and protest action, and those strikes and protest action in violation of the Act, namely, ‘unprotected’ strikes and protest action. Participation in an unprotected strike is one form of misbehaviour. The Labour Relations Act expressly prohibits the dismissal of employees engaged in a lawful strike. Employees engaged in strike action contrary to the provisions of the Labour Relations Act may be dismissed since their strike action is deemed to be a form of misconduct. The dismissal of striking employees must be both substantially and procedurally fair.
- Full Text:
- Date Issued: 2003
‘South Africa's Vietnam’? Literary History and Cultural Memory of the Border War
- Authors: Baines, Gary F
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/126987 , vital:35941 , https://doi.10.1080/17533170400505305
- Description: Density, population structure, growth, mortality and aspects of reproduction of the non-native amphipod Platorchestia platensis were studied for 20 consecutive months (October 2008 to May 2010) in the supralittoral wrack of the Knysna Estuary, South Africa. Amphipod density varied over the sampling period with the lowest numbers recorded in summer (January and February). Ovigerous females with embryos, and juveniles were found in most months with peaks in recruitment found in both April and October, suggesting that in Knysna this species is a biannual breeder. Average female size was significantly greater in winter, with larger females tending to brood more embryos. The largest adults (13.5 mm total length) were always male although the monthly sex ratio was nearly always female biased. Growth rate estimated from a cohort analysis was about 1 mm per month and monthly survival about 69%. The ability to reproduce all year round may be one reason why this introduced species has become established within this warm temperate estuary.
- Full Text:
- Date Issued: 2003
- Authors: Baines, Gary F
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/126987 , vital:35941 , https://doi.10.1080/17533170400505305
- Description: Density, population structure, growth, mortality and aspects of reproduction of the non-native amphipod Platorchestia platensis were studied for 20 consecutive months (October 2008 to May 2010) in the supralittoral wrack of the Knysna Estuary, South Africa. Amphipod density varied over the sampling period with the lowest numbers recorded in summer (January and February). Ovigerous females with embryos, and juveniles were found in most months with peaks in recruitment found in both April and October, suggesting that in Knysna this species is a biannual breeder. Average female size was significantly greater in winter, with larger females tending to brood more embryos. The largest adults (13.5 mm total length) were always male although the monthly sex ratio was nearly always female biased. Growth rate estimated from a cohort analysis was about 1 mm per month and monthly survival about 69%. The ability to reproduce all year round may be one reason why this introduced species has become established within this warm temperate estuary.
- Full Text:
- Date Issued: 2003
The importance of managing cultural change in the succession process within family businesses in the Gauteng area
- Authors: Hynd, Dale Vaughan
- Date: 2003
- Subjects: Family-owned business enterprises -- South Africa Family-owned business enterprises -- Succession Corporate culture -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3173 , http://hdl.handle.net/10962/d1007802
- Description: The aim of this research was to ascertain the implications and the impact that the succession process would have on the organisational culture of family businesses. In an attempt to answer this, the researcher asked three questions surrounding the succession process and its relationship with organisational culture. The questions related to the extent to which the family business was prepared for change in management; what changes occurred before, during, and immediately after the succession process, and what results these changes had on the organisational culture. Family businesses account for a large proportion of the national and global market activities, and so it is imperative that attention be paid to any problems they may experience. As it is, family businesses have difficulty in successions, with roughly one third of first generation family businesses surviving the succession process. It is the aim of this research to explore the contribution organisational culture has in the large failure rate of succession with family businesses. When successors enter into the organisation, they bring with them different perspectives on managerial issues suggesting that a change in leadership style will occur as a result of a succession. In answering the research questions, the researcher embarked on a two-phase research methodology utilising a quantitative and qualitative process. This triangulation process incorporates a self-administrated survey questionnaire, and six in-depth interviews. The survey questionnaire and interview schedules were structured using a combination of elements obtained from Harris's (1998) and Levinson's (1972). The survey data was analysed using various statistical methods, predominantly a Factor Analysis, where as Abstract iii the interviews were analysed using a theme retrieval process. The two processes were combined to yield the results. The findings of the research conclude that through the introduction of the successor in the change process, a new leadership style is introduced into the business. The new leader changes policies, practices and procedures, which are related to organisational climate and are the tangible aspects of organisational culture. Altering the climate essentially alters the culture, which may result in anxiety within the organisation and lead to tension. If these issues are not addressed, they may result in the demise of family businesses.
- Full Text:
- Date Issued: 2003
- Authors: Hynd, Dale Vaughan
- Date: 2003
- Subjects: Family-owned business enterprises -- South Africa Family-owned business enterprises -- Succession Corporate culture -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3173 , http://hdl.handle.net/10962/d1007802
- Description: The aim of this research was to ascertain the implications and the impact that the succession process would have on the organisational culture of family businesses. In an attempt to answer this, the researcher asked three questions surrounding the succession process and its relationship with organisational culture. The questions related to the extent to which the family business was prepared for change in management; what changes occurred before, during, and immediately after the succession process, and what results these changes had on the organisational culture. Family businesses account for a large proportion of the national and global market activities, and so it is imperative that attention be paid to any problems they may experience. As it is, family businesses have difficulty in successions, with roughly one third of first generation family businesses surviving the succession process. It is the aim of this research to explore the contribution organisational culture has in the large failure rate of succession with family businesses. When successors enter into the organisation, they bring with them different perspectives on managerial issues suggesting that a change in leadership style will occur as a result of a succession. In answering the research questions, the researcher embarked on a two-phase research methodology utilising a quantitative and qualitative process. This triangulation process incorporates a self-administrated survey questionnaire, and six in-depth interviews. The survey questionnaire and interview schedules were structured using a combination of elements obtained from Harris's (1998) and Levinson's (1972). The survey data was analysed using various statistical methods, predominantly a Factor Analysis, where as Abstract iii the interviews were analysed using a theme retrieval process. The two processes were combined to yield the results. The findings of the research conclude that through the introduction of the successor in the change process, a new leadership style is introduced into the business. The new leader changes policies, practices and procedures, which are related to organisational climate and are the tangible aspects of organisational culture. Altering the climate essentially alters the culture, which may result in anxiety within the organisation and lead to tension. If these issues are not addressed, they may result in the demise of family businesses.
- Full Text:
- Date Issued: 2003
The experience of being diagnosed with a psychiatric disorder: living the label
- Knight, Zelda G, Bradfield, Bruce
- Authors: Knight, Zelda G , Bradfield, Bruce
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6262 , http://hdl.handle.net/10962/d1007880
- Description: Informed by the investigative thrust of phenomenological inquiry and the 'phenomenology of intersubjectivity', the overarching aim of this article is to provide an accurate illumination of the experience of being diagnosed with a psychiatric disorder, and thus being 'a labelled individual'. This article is based on research that sought to understand the impact of the psychiatric label upon labelled individuals interpersonal and intersubjective presence as experienced outside the psychiatric institution. The principle question asked was: "What is the experience of being a labelled individual in the world?". It was discovered that psychiatric labelling unfolds as a disconnection and dislocation from co-existence with others. Moreover, labelling had the effect of robbing such individuals of their subjectivity, rendering them lonely, misunderstood and viewed as somehow defective, disabled and wrong.
- Full Text:
- Date Issued: 2003
- Authors: Knight, Zelda G , Bradfield, Bruce
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6262 , http://hdl.handle.net/10962/d1007880
- Description: Informed by the investigative thrust of phenomenological inquiry and the 'phenomenology of intersubjectivity', the overarching aim of this article is to provide an accurate illumination of the experience of being diagnosed with a psychiatric disorder, and thus being 'a labelled individual'. This article is based on research that sought to understand the impact of the psychiatric label upon labelled individuals interpersonal and intersubjective presence as experienced outside the psychiatric institution. The principle question asked was: "What is the experience of being a labelled individual in the world?". It was discovered that psychiatric labelling unfolds as a disconnection and dislocation from co-existence with others. Moreover, labelling had the effect of robbing such individuals of their subjectivity, rendering them lonely, misunderstood and viewed as somehow defective, disabled and wrong.
- Full Text:
- Date Issued: 2003
Those merry, tinkling, tuneful bells : handbells in Victorian Grahamstown with a note on bell ringing at Grahamstown Cathedral
- Authors: Berning, J M
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6993 , http://hdl.handle.net/10962/d1012420
- Description: [From the introduction]: The idea of using sets of small bells tuned to particular notes in order to produce music is very old. There are illustrations of slung bells being played in this way from the 11th and 12th centuries. Bell ringers in England date the sue of sets of tuned and hand-held bells from as early as the 16th century though it seems that the modern handbell may have come into existence in the early 18th century. Such bells were used by tower bell ringers as convenient practice devices for change ringing. The ringing of tunes on handbells became popular in the 18th century and reached its heyday in the latter half of the 19th century. In England tune ringing was especially popular in the north and major competitions had their centre at Manchester. Special trains were run to competitions there and bands, ringing up to 200 bells, could find their skills tested on extracts from Mozart's Don Giovanni. World War I and the spread of alternative media of entertainment like radio put an end to ringing on this scale. , This publication marked the 150th Anniversary of the Diocese of Grahamstown. Michael Berning was a member of the Rhodes University Library staff from 1965 until his retirement in 1997. He was Tower Captain of the Grahamstown Cathedral during the 1980s.
- Full Text:
- Date Issued: 2003
- Authors: Berning, J M
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6993 , http://hdl.handle.net/10962/d1012420
- Description: [From the introduction]: The idea of using sets of small bells tuned to particular notes in order to produce music is very old. There are illustrations of slung bells being played in this way from the 11th and 12th centuries. Bell ringers in England date the sue of sets of tuned and hand-held bells from as early as the 16th century though it seems that the modern handbell may have come into existence in the early 18th century. Such bells were used by tower bell ringers as convenient practice devices for change ringing. The ringing of tunes on handbells became popular in the 18th century and reached its heyday in the latter half of the 19th century. In England tune ringing was especially popular in the north and major competitions had their centre at Manchester. Special trains were run to competitions there and bands, ringing up to 200 bells, could find their skills tested on extracts from Mozart's Don Giovanni. World War I and the spread of alternative media of entertainment like radio put an end to ringing on this scale. , This publication marked the 150th Anniversary of the Diocese of Grahamstown. Michael Berning was a member of the Rhodes University Library staff from 1965 until his retirement in 1997. He was Tower Captain of the Grahamstown Cathedral during the 1980s.
- Full Text:
- Date Issued: 2003
Effect of pictograms on readability of patient information materials:
- Mansoor, Leila E, Dowse, Roslind
- Authors: Mansoor, Leila E , Dowse, Roslind
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/157003 , vital:40078 , https://0-doi.org.wam.seals.ac.za/10.1345/aph.1C449
- Description: To design, develop, and evaluate a simple, understandable medicine label and patient information leaflet (PIL) for nystatin suspension, and to assess the effect of incorporating pictograms on understanding in low-literate participants.
- Full Text:
- Date Issued: 2003
- Authors: Mansoor, Leila E , Dowse, Roslind
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/157003 , vital:40078 , https://0-doi.org.wam.seals.ac.za/10.1345/aph.1C449
- Description: To design, develop, and evaluate a simple, understandable medicine label and patient information leaflet (PIL) for nystatin suspension, and to assess the effect of incorporating pictograms on understanding in low-literate participants.
- Full Text:
- Date Issued: 2003
Molecular identification of some forensically important blowflies of southern Africa and Australia
- Harvey, M L, Mansell, M W, Villet, Martin H, Dadour, I R
- Authors: Harvey, M L , Mansell, M W , Villet, Martin H , Dadour, I R
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6953 , http://hdl.handle.net/10962/d1011986
- Description: One major aspect of research in forensic entomology is the investigation of molecular techniques for the accurate identification of insects. Studies to date have addressed the corpse fauna of many geographical regions, but generally neglected the southern African calliphorid species. In this study, forensically significant calliphorids from South Africa, Swaziland, Botswana and Zimbabwe and Australia were sequenced over an 1167 base pair region of the COI gene. Phylogenetic analysis was performed to examine the ability of the region to resolve species identities and taxonomic relationships between species. Analyses by neighbour-joining, maximum parsimony and maximum likelihood methods all showed the potential of this region to provide the necessary species-level identifications for application to post-mortem interval (PMI) estimation; however, higher level taxonomic relationships did vary according to method of analysis. Intraspecific variation was also considered in relation to determining suitable maximum levels of variation to be expected during analysis. Individuals of some species in the study represented populations from both South Africa and the east coast of Australia, yet maximum intraspecific variation over this gene region was calculated at 0.8%, with minimum interspecific variation at 3%, indicating distinct ranges of variation to be expected at intra- and interspecific levels. This region therefore appears to provide southern African forensic entomologists with a new technique for providing accurate identification for application to estimation of PMI.
- Full Text:
- Date Issued: 2003
- Authors: Harvey, M L , Mansell, M W , Villet, Martin H , Dadour, I R
- Date: 2003
- Language: English
- Type: text , Article
- Identifier: vital:6953 , http://hdl.handle.net/10962/d1011986
- Description: One major aspect of research in forensic entomology is the investigation of molecular techniques for the accurate identification of insects. Studies to date have addressed the corpse fauna of many geographical regions, but generally neglected the southern African calliphorid species. In this study, forensically significant calliphorids from South Africa, Swaziland, Botswana and Zimbabwe and Australia were sequenced over an 1167 base pair region of the COI gene. Phylogenetic analysis was performed to examine the ability of the region to resolve species identities and taxonomic relationships between species. Analyses by neighbour-joining, maximum parsimony and maximum likelihood methods all showed the potential of this region to provide the necessary species-level identifications for application to post-mortem interval (PMI) estimation; however, higher level taxonomic relationships did vary according to method of analysis. Intraspecific variation was also considered in relation to determining suitable maximum levels of variation to be expected during analysis. Individuals of some species in the study represented populations from both South Africa and the east coast of Australia, yet maximum intraspecific variation over this gene region was calculated at 0.8%, with minimum interspecific variation at 3%, indicating distinct ranges of variation to be expected at intra- and interspecific levels. This region therefore appears to provide southern African forensic entomologists with a new technique for providing accurate identification for application to estimation of PMI.
- Full Text:
- Date Issued: 2003
The biogeography of the Prosopistomatidae, with a particular emphasis on Southern African species
- Authors: Barber-James, Helen M
- Date: 2003
- Language: English
- Type: Conference paper
- Identifier: vital:6998 , http://hdl.handle.net/10962/d1008378
- Description: The mayfly family Prosopistomatidae consists of the single genus Prosopistoma Latreille. Its known distribution includes species from Africa, Madagascar, the Comores, Europe, the Levant, India, Sri Lanka, China, Vietnam, the Philippines, Malaysia, Sumatra, Java, the Solomon Islands, Papua New Guinea and northern Australia. A tropical Gondwanaland origin of the family has been suggested. No species are currently known from the Neotropical or Nearctic regions, though the family may yet be discovered in northeastern South America, since this region separated from West Africa only c.120 mya. Focussing on southern Africa, several undescribed species have recently been discovered, with interesting implications to the biogeography on a more localised scale. In the western Cape, a prosopistomatid species has been collected in the Olifants River, extending the distribution of this family further south into a more temperate region. Geological evidence indicates that the Olifants River was connected to the Orange River during the Tertiary period. Prosopistomatidae are known from the Orange River today, and the presence of the family in the Olifants River in the western Cape supports the geological evidence of the historical link between these two rivers. Another unexpected discovery was from the Buffalo River in the eastern Cape, at 33ºS. A subtropical zone extends along the east coast of South Africa as a result of the warm Agulhas current offshore, allowing the southerly extension of the distribution of more tropical species. , Research Update on Ephemeroptera & Plecoptera: Proceedings of the Tenth International Conference on Ephemeroptera, 8-11 August 2001, Perugia, Italy. University of Perugia, Perugia, Italy, pp. 263-270.
- Full Text:
- Date Issued: 2003
- Authors: Barber-James, Helen M
- Date: 2003
- Language: English
- Type: Conference paper
- Identifier: vital:6998 , http://hdl.handle.net/10962/d1008378
- Description: The mayfly family Prosopistomatidae consists of the single genus Prosopistoma Latreille. Its known distribution includes species from Africa, Madagascar, the Comores, Europe, the Levant, India, Sri Lanka, China, Vietnam, the Philippines, Malaysia, Sumatra, Java, the Solomon Islands, Papua New Guinea and northern Australia. A tropical Gondwanaland origin of the family has been suggested. No species are currently known from the Neotropical or Nearctic regions, though the family may yet be discovered in northeastern South America, since this region separated from West Africa only c.120 mya. Focussing on southern Africa, several undescribed species have recently been discovered, with interesting implications to the biogeography on a more localised scale. In the western Cape, a prosopistomatid species has been collected in the Olifants River, extending the distribution of this family further south into a more temperate region. Geological evidence indicates that the Olifants River was connected to the Orange River during the Tertiary period. Prosopistomatidae are known from the Orange River today, and the presence of the family in the Olifants River in the western Cape supports the geological evidence of the historical link between these two rivers. Another unexpected discovery was from the Buffalo River in the eastern Cape, at 33ºS. A subtropical zone extends along the east coast of South Africa as a result of the warm Agulhas current offshore, allowing the southerly extension of the distribution of more tropical species. , Research Update on Ephemeroptera & Plecoptera: Proceedings of the Tenth International Conference on Ephemeroptera, 8-11 August 2001, Perugia, Italy. University of Perugia, Perugia, Italy, pp. 263-270.
- Full Text:
- Date Issued: 2003
The vegetation of the habitat of the Brenton blue butterfly, Orachrysops niobe (Trimen), in the Western Cape, South Africa
- Lubke, Roy, Hoare, D, Victor, J, Ketelaar, R
- Authors: Lubke, Roy , Hoare, D , Victor, J , Ketelaar, R
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6524 , http://hdl.handle.net/10962/d1005957
- Description: The Brenton blue butterfly is known only from a small population in one hectare of asteraceous coastal fynbos at Brenton-on-Sea. This fynbos is characterized by a great diversity of shrubs, herbs and graminoids, with a successional gradient to thicket where Pterocelastrus tricuspidatus is dominant. The eggs of the butterfly are laid on the lower side of the leaves of Indigofera erecta, on which the larvae feed. Fifteen 1-m² quadrats containing plants of Indigofera erecta with and without eggs of the butterfly were distinguished and sampled separately from 15 1-m² quadrats containing plants of Indigofera erecta without eggs. No marked differences in total vegetation, shrub or herb cover between the sites with and without eggs were observed. There was a difference in abundance of the fern Pteridium aquilinum, with over 30% cover at sites with no eggs and only about 6% at sites with eggs present. This could reflect the absence of other plants where the ferns had such dense cover.
- Full Text:
- Date Issued: 2003
- Authors: Lubke, Roy , Hoare, D , Victor, J , Ketelaar, R
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6524 , http://hdl.handle.net/10962/d1005957
- Description: The Brenton blue butterfly is known only from a small population in one hectare of asteraceous coastal fynbos at Brenton-on-Sea. This fynbos is characterized by a great diversity of shrubs, herbs and graminoids, with a successional gradient to thicket where Pterocelastrus tricuspidatus is dominant. The eggs of the butterfly are laid on the lower side of the leaves of Indigofera erecta, on which the larvae feed. Fifteen 1-m² quadrats containing plants of Indigofera erecta with and without eggs of the butterfly were distinguished and sampled separately from 15 1-m² quadrats containing plants of Indigofera erecta without eggs. No marked differences in total vegetation, shrub or herb cover between the sites with and without eggs were observed. There was a difference in abundance of the fern Pteridium aquilinum, with over 30% cover at sites with no eggs and only about 6% at sites with eggs present. This could reflect the absence of other plants where the ferns had such dense cover.
- Full Text:
- Date Issued: 2003
Small-scale fisheries as a vehicle for rural development : a case study of two villages in the former Ciskei, Eastern Cape Province, South Africa
- Authors: Seti, Simpiwe
- Date: 2003
- Subjects: Small-scale fisheries -- South Africa -- Eastern Cape Fisheries -- South Africa -- Eastern Cape Fishes -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Fish trade -- Developing countries Fisheries, Cooperative -- Developing countries
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3346 , http://hdl.handle.net/10962/d1006016
- Description: This thesis examines small-scale rural fisheries which were initiated in two villages by the Department of Ichthyology and Fisheries in conjunction with the Institute of Social and Economic Research in 1997. The main objective for undertaking the study was to determine the potential of the fisheries to serve as viable and sustainable options that could contribute towards rural development in the area of study. To examine the contribution made by the small-scale fisheries towards rural development in the villages, it is imperative to have an understanding of the context in which they were established as well as the prospects for sustainable development. It is argued in this study that despite the significant contribution made by inland rural fisheries in improving rural livelihoods they are still accorded minimal support and attention in South Africa. As an illustration of the important role played by rural fisheries, case studies from Malawi and Zimbabwe have been drawn into the study with a view to providing lessons for rural areas of South Africa. The study took place at a time when natural resources in the villages were significantly declining and environmental degradation reaching uncontrollable proportions, so there is an urgent need to secure an alternative mode of subsistence for the population in the villages. Development interventions by the state and non-governmental organisations in the study area have not been sustainable and they collapsed within a short period of time. A case in point is the Tyefu irrigation scheme, which was initiated in the villages but, owing to various factors, it failed leaving the villagers with a diminished mode of subsistence, thus providing the impetus for the implementation of small-scale rural fisheries. In order for small-scale fisheries to be sustainable it is essential to adopt models that are aimed at sound natural resource use and management. This study has raised various issues pertinent to natural resource use and management in the study area. A model for management of natural resources that involves and aims to empower local communities in the management of the fisheries is presented in the study. It has also been demonstrated that rural fisheries in the study area comply with the model. Finally, the study draws the conclusion that small-scale fisheries in the villages contribute towards rural development, however, there are constraints that need to be eradicated.
- Full Text:
- Date Issued: 2003
- Authors: Seti, Simpiwe
- Date: 2003
- Subjects: Small-scale fisheries -- South Africa -- Eastern Cape Fisheries -- South Africa -- Eastern Cape Fishes -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Fish trade -- Developing countries Fisheries, Cooperative -- Developing countries
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3346 , http://hdl.handle.net/10962/d1006016
- Description: This thesis examines small-scale rural fisheries which were initiated in two villages by the Department of Ichthyology and Fisheries in conjunction with the Institute of Social and Economic Research in 1997. The main objective for undertaking the study was to determine the potential of the fisheries to serve as viable and sustainable options that could contribute towards rural development in the area of study. To examine the contribution made by the small-scale fisheries towards rural development in the villages, it is imperative to have an understanding of the context in which they were established as well as the prospects for sustainable development. It is argued in this study that despite the significant contribution made by inland rural fisheries in improving rural livelihoods they are still accorded minimal support and attention in South Africa. As an illustration of the important role played by rural fisheries, case studies from Malawi and Zimbabwe have been drawn into the study with a view to providing lessons for rural areas of South Africa. The study took place at a time when natural resources in the villages were significantly declining and environmental degradation reaching uncontrollable proportions, so there is an urgent need to secure an alternative mode of subsistence for the population in the villages. Development interventions by the state and non-governmental organisations in the study area have not been sustainable and they collapsed within a short period of time. A case in point is the Tyefu irrigation scheme, which was initiated in the villages but, owing to various factors, it failed leaving the villagers with a diminished mode of subsistence, thus providing the impetus for the implementation of small-scale rural fisheries. In order for small-scale fisheries to be sustainable it is essential to adopt models that are aimed at sound natural resource use and management. This study has raised various issues pertinent to natural resource use and management in the study area. A model for management of natural resources that involves and aims to empower local communities in the management of the fisheries is presented in the study. It has also been demonstrated that rural fisheries in the study area comply with the model. Finally, the study draws the conclusion that small-scale fisheries in the villages contribute towards rural development, however, there are constraints that need to be eradicated.
- Full Text:
- Date Issued: 2003
Locating the institution of traditional leadership within the institutional framework of South Africa's new democracy
- Authors: Mashele, Hlukanisa Prince
- Date: 2003
- Subjects: Tribal government -- South Africa , Political leadership -- South Africa , South Africa -- Politics and government -- 1994-
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2858 , http://hdl.handle.net/10962/d1007512 , Tribal government -- South Africa , Political leadership -- South Africa , South Africa -- Politics and government -- 1994-
- Description: This study looks into the role of the institution of traditional leadership in post-apartheid South Africa. It seeks to critically engage the debate on how to locate the institution within the new politico-constitutional framework. This is done with the main objective of proposing an altemative to the current state of affairs vis-à-vis traditional leadership and governance in South Africa. In order to clear the ground, the study first deals with the important question of democracy in relation to the institution of traditional leadership. In this regard, the study unearthed that the institution of traditional leadership is fundamentally undemocratic in character, as it is largely based on heredity and devoid of principles of democracy such as equality, accountability, etc. In order to put matters into perspective the study also delves into the history of the institution of traditional leadership with the aim of getting to the role that traditional leaders played in various epochs of South Africa's political development. This investigation reveals that the denting of the integrity of the institution of traditional leadership began with the advent of colonialism and worsened by successive apartheid regimes. It is at these stages of development that the institution was subordinated to a higher authority that sought to use the institution as an instrument of domination and oppression of the black majority. Considering this role, it would seem that the place that the space that the institution occupies in the post-apartheid South African governance framework is a compromise. The institution plays an advisory role at all levels of government - with their houses in both national and provincial legislatures, whilst traditional leaders sit as ex-officio members on local councils. However, traditional leaders fiercely contest this position as, in their view, this limits their powers. The main argument of this study is that for traditional leaders to be given an advisory role in the current and future governance framework of the country is a step in the right direction, as that serves to insulate the institution from active politics. For that reason, the study recommends that the institution of traditional leadership should occupy a cultural space in society - meaning that it should be responsible for the preservation of African customs and culture. This, therefore, means that the institution is better-placed to advise government on cultural and customary aspects of development. Whilst playing this role, the institution of traditional leadership should also -be brought into line with democratic ways of governance.
- Full Text:
- Date Issued: 2003
- Authors: Mashele, Hlukanisa Prince
- Date: 2003
- Subjects: Tribal government -- South Africa , Political leadership -- South Africa , South Africa -- Politics and government -- 1994-
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2858 , http://hdl.handle.net/10962/d1007512 , Tribal government -- South Africa , Political leadership -- South Africa , South Africa -- Politics and government -- 1994-
- Description: This study looks into the role of the institution of traditional leadership in post-apartheid South Africa. It seeks to critically engage the debate on how to locate the institution within the new politico-constitutional framework. This is done with the main objective of proposing an altemative to the current state of affairs vis-à-vis traditional leadership and governance in South Africa. In order to clear the ground, the study first deals with the important question of democracy in relation to the institution of traditional leadership. In this regard, the study unearthed that the institution of traditional leadership is fundamentally undemocratic in character, as it is largely based on heredity and devoid of principles of democracy such as equality, accountability, etc. In order to put matters into perspective the study also delves into the history of the institution of traditional leadership with the aim of getting to the role that traditional leaders played in various epochs of South Africa's political development. This investigation reveals that the denting of the integrity of the institution of traditional leadership began with the advent of colonialism and worsened by successive apartheid regimes. It is at these stages of development that the institution was subordinated to a higher authority that sought to use the institution as an instrument of domination and oppression of the black majority. Considering this role, it would seem that the place that the space that the institution occupies in the post-apartheid South African governance framework is a compromise. The institution plays an advisory role at all levels of government - with their houses in both national and provincial legislatures, whilst traditional leaders sit as ex-officio members on local councils. However, traditional leaders fiercely contest this position as, in their view, this limits their powers. The main argument of this study is that for traditional leaders to be given an advisory role in the current and future governance framework of the country is a step in the right direction, as that serves to insulate the institution from active politics. For that reason, the study recommends that the institution of traditional leadership should occupy a cultural space in society - meaning that it should be responsible for the preservation of African customs and culture. This, therefore, means that the institution is better-placed to advise government on cultural and customary aspects of development. Whilst playing this role, the institution of traditional leadership should also -be brought into line with democratic ways of governance.
- Full Text:
- Date Issued: 2003
Intervention in Bosnia-Herzegovina: lessons of peace‐maintenance for Africa?
- Authors: Bischoff, Paul, 1954-
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/161365 , vital:40620 , DOI: 10.1080/10220460309545430
- Description: The Bosnian model is potentially a useful model for the development of a new strategic framework for African conflicts, particularly in situations where international organisations such as the United Nations are overstretched and regional organisations wish to take charge of peacebuilding operations.
- Full Text:
- Date Issued: 2003
- Authors: Bischoff, Paul, 1954-
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/161365 , vital:40620 , DOI: 10.1080/10220460309545430
- Description: The Bosnian model is potentially a useful model for the development of a new strategic framework for African conflicts, particularly in situations where international organisations such as the United Nations are overstretched and regional organisations wish to take charge of peacebuilding operations.
- Full Text:
- Date Issued: 2003
Applied studies of some Southern African blowflies (Diptera: Calliphoridae) of forensic importance
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Date Issued: 2003
- Authors: Lunt, Nicola
- Date: 2003
- Subjects: Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5803 , http://hdl.handle.net/10962/d1006202 , Diptera -- South Africa , Blowflies -- South Africa , Forensic entomology
- Description: Three major aspects of blowfly (Diptera: Calliphoridae) research were the focus of this study. Firstly, the phylogenetic relationships of 40 oestroid species from a variety of geographical localities were investigated using Cytochrome Oxidase b subunit I (COl) gene sequences. Maximum parsimony (MP) and Jukes-Cantor neighbor-joining (NJ) analyses both extracted a paraphyletic Calliphoridae, with the Calliphorinae-Luciliinae clade being sister to the Sarcophagidae. Short branch lengths within Chrysomya indicate a recent rapid radiation of this genus. Phormia and Protophormia either formed a sister clade to Chrysomya, or were embedded in this genus. Tree topologies were comparable between MP and NJ trees, but the positions of some genera were ambiguous. Secondly, developmental parameters and behaviour were investigated for four southern African species of forensically important blowflies viz. Chrysomya chloropyga, C. putoria, C. megacephala and Lucilia sericata, and ad hoc observations were made for Calliphora croceipalpis, Chrysomya marginalis and the predatory C. albiceps. Choice of oviposition substrate differed between species, mirroring substrate preferences in the field. Sexual dimorphism and dwarfism within a cohort complicated ageing maggots using size, but the use of developmental events (e.g. ecdysis) allowed ages to be determined unambiguously. Separate species status was supported for the previously synonymised C. chloropyga and C. putoria, by differences in maggot behaviour, larval growth rates and temperature optima. The proportion of total development time assigned to each larval instar and pupariation was variable among temperatures, but similar between congeneric species. Thirdly, since a negative linear relationship was found to occur between the developmental constant (K) and developmental zero (D₀) for both Calliphoridae and Sarcophagidae, the potential for predicting physiological parameters of unstudied taxa was investigated. Species and genera of Palaearctic origin generally had high K's and low D₀'s, and the reverse was true for the tropical taxa. It was found that both K and D₀ can be estimated for "unknown" taxa using the Felsenstein's Independent Contrasts (FIC) method of PDTree (Garland et al. 200 I), provided that branch lengths are relatively short and the phylogenetic position of the estimated taxon is unambiguous.
- Full Text:
- Date Issued: 2003
Using E-learning to support IT education in a university environment a case study approach
- Authors: Taljaard, Marinda
- Date: 2003
- Subjects: Education, Higher -- South Africa -- Port Elizabeth -- Computer-assisted instruction , Internet in education , Information technology -- Study and teaching (Higher) -- South Africa -- Port Elizabeth , College teaching -- South Africa -- Port Elizabeth -- Computer network resources , University of Port Elizabeth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11091 , http://hdl.handle.net/10948/d1015740 , Education, Higher -- South Africa -- Port Elizabeth -- Computer-assisted instruction , Internet in education , Information technology -- Study and teaching (Higher) -- South Africa -- Port Elizabeth , College teaching -- South Africa -- Port Elizabeth -- Computer network resources , University of Port Elizabeth
- Description: At the University of Port Elizabeth (UPE), the End User Computing course (EUC) acts as a service course for many departments. This implies that many students are forced by their curricula to register for this course. The ever-increasing numbers in EUC place a considerable load on existing human and physical resources. In lecture groups of 120 –160, students rarely get the attention they need, and the pace at which the content is delivered (too slow or too fast) may also inhibit the learning process. During an initial investigation into E-learning at UPE in 1999, a prototype virtual classroom was developed. There were, however, a number of problems with this prototype. Firstly, it was implemented using a number of different technologies, which made it difficult to extend and maintain. Secondly, it only addressed some aspects of an E-learning environment, which proved insufficient for the EUC course. In the existing EUC course at UPE, the students are already exposed to some E-learning concepts, as a section of their skills training component is handled by using multimedia software in a simulated environment. The objective of this project was to extend the E-learning component further to determine the advantages and disadvantages of using E-learning to support information technology (IT) education in a contact-university environment. This project included a literature search and survey of existing E-learning environments at other universities. This research was used to develop a draft framework for an E-learning environment. The framework was used to select a tool to create an E-learning environment at UPE. An experiment was designed using this E-learning environment to support two IT courses at different year levels. The results of the experiment were analysed using qualitative and quantitative methods to determine the impact of using E-learning to support IT education at UPE. The results of this research show that E-learning can be used to support IT education at UPE. More success, however, was achieved at postgraduate level than at first-year level. Making use of Elearning increased student satisfaction and promoted active learning, while providing benefits like convenience, communication, flexibility and scaffolding. We conclude, therefore, that E-learning can provide a flexible approach to IT education in a university environment in the future.
- Full Text:
- Date Issued: 2003
- Authors: Taljaard, Marinda
- Date: 2003
- Subjects: Education, Higher -- South Africa -- Port Elizabeth -- Computer-assisted instruction , Internet in education , Information technology -- Study and teaching (Higher) -- South Africa -- Port Elizabeth , College teaching -- South Africa -- Port Elizabeth -- Computer network resources , University of Port Elizabeth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:11091 , http://hdl.handle.net/10948/d1015740 , Education, Higher -- South Africa -- Port Elizabeth -- Computer-assisted instruction , Internet in education , Information technology -- Study and teaching (Higher) -- South Africa -- Port Elizabeth , College teaching -- South Africa -- Port Elizabeth -- Computer network resources , University of Port Elizabeth
- Description: At the University of Port Elizabeth (UPE), the End User Computing course (EUC) acts as a service course for many departments. This implies that many students are forced by their curricula to register for this course. The ever-increasing numbers in EUC place a considerable load on existing human and physical resources. In lecture groups of 120 –160, students rarely get the attention they need, and the pace at which the content is delivered (too slow or too fast) may also inhibit the learning process. During an initial investigation into E-learning at UPE in 1999, a prototype virtual classroom was developed. There were, however, a number of problems with this prototype. Firstly, it was implemented using a number of different technologies, which made it difficult to extend and maintain. Secondly, it only addressed some aspects of an E-learning environment, which proved insufficient for the EUC course. In the existing EUC course at UPE, the students are already exposed to some E-learning concepts, as a section of their skills training component is handled by using multimedia software in a simulated environment. The objective of this project was to extend the E-learning component further to determine the advantages and disadvantages of using E-learning to support information technology (IT) education in a contact-university environment. This project included a literature search and survey of existing E-learning environments at other universities. This research was used to develop a draft framework for an E-learning environment. The framework was used to select a tool to create an E-learning environment at UPE. An experiment was designed using this E-learning environment to support two IT courses at different year levels. The results of the experiment were analysed using qualitative and quantitative methods to determine the impact of using E-learning to support IT education at UPE. The results of this research show that E-learning can be used to support IT education at UPE. More success, however, was achieved at postgraduate level than at first-year level. Making use of Elearning increased student satisfaction and promoted active learning, while providing benefits like convenience, communication, flexibility and scaffolding. We conclude, therefore, that E-learning can provide a flexible approach to IT education in a university environment in the future.
- Full Text:
- Date Issued: 2003
An investigation into the application of the IEEE 1394 high performance serial bus to sound installation contro
- Authors: Klinkradt, Bradley Hugh
- Date: 2003 , 2013-05-24
- Subjects: Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4612 , http://hdl.handle.net/10962/d1004899 , Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Description: This thesis investigates the feasibility of using existing IP-based control and monitoring protocols within professional audio installations utilising IEEE 1394 technology. Current control and monitoring technologies are examined, and the characteristics common to all are extracted and compiled into an object model. This model forms the foundation for a set of evaluation criteria against which current and future control and monitoring protocols may be measured. Protocols considered include AV/C, MIDI, QSC-24, and those utilised within the UPnP architecture. As QSC-24 and the UPnP architecture are IP-based, the facilities required to transport IP datagrams over the IEEE 1394 bus are investigated and implemented. Example QSC-24 and UPnP architecture implementations are described, which permit the control and monitoring of audio devices over the IEEE 1394 network using these IP-based technologies. The way forward for the control and monitoring of professional audio devices within installations is considered, and recommendations are provided. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2003
- Authors: Klinkradt, Bradley Hugh
- Date: 2003 , 2013-05-24
- Subjects: Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4612 , http://hdl.handle.net/10962/d1004899 , Digital electronics , Sound -- Recording and reproducing -- Digital techniques , Music -- Data processing , Computer sound processing
- Description: This thesis investigates the feasibility of using existing IP-based control and monitoring protocols within professional audio installations utilising IEEE 1394 technology. Current control and monitoring technologies are examined, and the characteristics common to all are extracted and compiled into an object model. This model forms the foundation for a set of evaluation criteria against which current and future control and monitoring protocols may be measured. Protocols considered include AV/C, MIDI, QSC-24, and those utilised within the UPnP architecture. As QSC-24 and the UPnP architecture are IP-based, the facilities required to transport IP datagrams over the IEEE 1394 bus are investigated and implemented. Example QSC-24 and UPnP architecture implementations are described, which permit the control and monitoring of audio devices over the IEEE 1394 network using these IP-based technologies. The way forward for the control and monitoring of professional audio devices within installations is considered, and recommendations are provided. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2003
The development of a model of competencies for small, medium and microsized enterprises (SMME'S) to achieve competitive advantage in the East Cape Motor Industry Cluster
- Authors: De Beer, Lloyd
- Date: 2003
- Subjects: Business enterprises -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10854 , http://hdl.handle.net/10948/121 , Business enterprises -- South Africa -- Eastern Cape
- Description: The research problem addressed in this study was to identify the generic strategies that small, medium and micro enterprises (SMME’s) are required to implement in order to achieve competitive advantage in the highly competitive global automotive market. Markets have merged into one huge global marketplace, increasing the competitive forces on all the participants in the automotive markets. Strategies to achieve competitive advantage has changed from the traditional domestic strategies to that of strategies required for global competitive advantage. This has created challenges for SMME’s to attain competitive advantage essential in the fast-changing global markets. The East Cape Motor Industry Cluster (ECMIC) is the heart of the SA automotive industry with three of the major automobile manufacturers having their assembly plants located in the Buffalo and Nelson Mandela Metropoles. A significant number of component manufacturers and their suppliers serve these manufacturers, as well as the other automobile manufacturers located elsewhere in South Africa. Many of these component manufacturers and suppliers are SMME’s. The need to become globally competitive is thus critical for SMME’s in the region. This study investigates the generic strategies that SMME organisations are required to implement in order to achieve competitive advantage in the ECMIC, and based on this and the empirical study that seeks opinion from management of SMME firms in the ECMIC, a model of generic strategies to create competitive advantage is developed.
- Full Text:
- Date Issued: 2003
- Authors: De Beer, Lloyd
- Date: 2003
- Subjects: Business enterprises -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10854 , http://hdl.handle.net/10948/121 , Business enterprises -- South Africa -- Eastern Cape
- Description: The research problem addressed in this study was to identify the generic strategies that small, medium and micro enterprises (SMME’s) are required to implement in order to achieve competitive advantage in the highly competitive global automotive market. Markets have merged into one huge global marketplace, increasing the competitive forces on all the participants in the automotive markets. Strategies to achieve competitive advantage has changed from the traditional domestic strategies to that of strategies required for global competitive advantage. This has created challenges for SMME’s to attain competitive advantage essential in the fast-changing global markets. The East Cape Motor Industry Cluster (ECMIC) is the heart of the SA automotive industry with three of the major automobile manufacturers having their assembly plants located in the Buffalo and Nelson Mandela Metropoles. A significant number of component manufacturers and their suppliers serve these manufacturers, as well as the other automobile manufacturers located elsewhere in South Africa. Many of these component manufacturers and suppliers are SMME’s. The need to become globally competitive is thus critical for SMME’s in the region. This study investigates the generic strategies that SMME organisations are required to implement in order to achieve competitive advantage in the ECMIC, and based on this and the empirical study that seeks opinion from management of SMME firms in the ECMIC, a model of generic strategies to create competitive advantage is developed.
- Full Text:
- Date Issued: 2003
Metallophthalocyanines as photocatalysts for transformation of chlorophenols and self-assembled monolayers for electrochemical detection of thiols and cyanides
- Authors: Ozoemena, Kenneth Ikechukwu
- Date: 2003
- Subjects: Electrochemistry Cyanides Thiols Chlorophenols Photocatalysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4089 , http://hdl.handle.net/10962/d1007709
- Description: Photochemical properties of sulphonated phthalocyanine complexes of aluminium, zinc, tin and silicon, and octa-carboxyphthalocyanine complexes of aluminium and zinc have been investigated. These water-soluble metallophthalocyanine (MPc) complexes, especially the sulphonated aluminium and zinc phthalocyanines, were found to be good photosensitisers for the transformation of the toxic mono-, tri- and penta-chlorophenols in aqueous solutions. The efficiency of MPc sensitiser towards photo-transformation of chlorophenols depends on its effectiveness to generate singlet oxygen as well as its photostability. Octa-substituted thiol-derivatised phthalocyanine complexes of cobalt, iron and zinc were synthesized and their spectral and electrochemical properties investigated. The photochemical properties ofthe zinc phthalocyanine complexes in non-aqueous solutions were comparable to those in literature. Ultrathin films of the octasubstituted thiol-derivatised phthalocyanine complexes of cobalt, iron and zinc were, for the first time, immobilized onto gold electrodes using the self-assembling technique. Surface electrochemistry indicates that the ultrathin films are surface-confined self-assembled monolayer (SAM) species. Gold electrodes modified with the redox-active SAMs of cobalt and iron phthalocyanine complexes proved to be potential electrochemical sensors for the detection of thiols (L-cysteine, homocysteine and penicillamine) and thiocyanate in aqueous solutions (pH 4). The limits of detection for the thiols and thiocyanate were in the range of ∼ 10⁻⁷ and 10⁻⁶ mol dm⁻³, respectively. The modification process was reproducible and the modified electrodes showed good stability and, if stored in pH 4 buffer solutions, could be used for the analysis of thiols and thiocyanate for about a month without the need for recalibration. Etching of gold marred electrochemical detection of cyanide with the MPc-SAM-modified gold electrodes. Interestingly, however, kinetic and equilibria studies revealed strong interaction of octabutylthiophthalocyaninatoiron (II), FeOBTPc, with cyanide in both DMF and DMSO solutions.
- Full Text:
- Date Issued: 2003
- Authors: Ozoemena, Kenneth Ikechukwu
- Date: 2003
- Subjects: Electrochemistry Cyanides Thiols Chlorophenols Photocatalysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4089 , http://hdl.handle.net/10962/d1007709
- Description: Photochemical properties of sulphonated phthalocyanine complexes of aluminium, zinc, tin and silicon, and octa-carboxyphthalocyanine complexes of aluminium and zinc have been investigated. These water-soluble metallophthalocyanine (MPc) complexes, especially the sulphonated aluminium and zinc phthalocyanines, were found to be good photosensitisers for the transformation of the toxic mono-, tri- and penta-chlorophenols in aqueous solutions. The efficiency of MPc sensitiser towards photo-transformation of chlorophenols depends on its effectiveness to generate singlet oxygen as well as its photostability. Octa-substituted thiol-derivatised phthalocyanine complexes of cobalt, iron and zinc were synthesized and their spectral and electrochemical properties investigated. The photochemical properties ofthe zinc phthalocyanine complexes in non-aqueous solutions were comparable to those in literature. Ultrathin films of the octasubstituted thiol-derivatised phthalocyanine complexes of cobalt, iron and zinc were, for the first time, immobilized onto gold electrodes using the self-assembling technique. Surface electrochemistry indicates that the ultrathin films are surface-confined self-assembled monolayer (SAM) species. Gold electrodes modified with the redox-active SAMs of cobalt and iron phthalocyanine complexes proved to be potential electrochemical sensors for the detection of thiols (L-cysteine, homocysteine and penicillamine) and thiocyanate in aqueous solutions (pH 4). The limits of detection for the thiols and thiocyanate were in the range of ∼ 10⁻⁷ and 10⁻⁶ mol dm⁻³, respectively. The modification process was reproducible and the modified electrodes showed good stability and, if stored in pH 4 buffer solutions, could be used for the analysis of thiols and thiocyanate for about a month without the need for recalibration. Etching of gold marred electrochemical detection of cyanide with the MPc-SAM-modified gold electrodes. Interestingly, however, kinetic and equilibria studies revealed strong interaction of octabutylthiophthalocyaninatoiron (II), FeOBTPc, with cyanide in both DMF and DMSO solutions.
- Full Text:
- Date Issued: 2003