Local perceptions of environmental changes in fishing communities of southwest Madagascar:
- Lemahieu, Anne, Scott, Lucy E P, Malherbe, Willem, Mahatante, Paubert T, Randrianarimanana, José V, Aswani, Shankar
- Authors: Lemahieu, Anne , Scott, Lucy E P , Malherbe, Willem , Mahatante, Paubert T , Randrianarimanana, José V , Aswani, Shankar
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/145370 , vital:38432 , DOI: 10.1016/j.ocecoaman.2018.06.012
- Description: Southwest Madagascar is a region that is significantly impacted upon by climate change. As in a lot of developing countries, the livelihoods of many communities in this region are dependent on fishing. This makes these communities particularly vulnerable to climate-related changes. We conducted a survey in two coastal fishing communities in the Toliara Province, Ambola and Ambotsibotsike. Using a free listing exercise, semi-structured interviews and focus group methods, we documented local perceptions of environmental changes and responses to changes. Results were compared, taking into account the differences in the degree of remoteness, market exposure and religiosity. Time periods that respondents reported as having had a high degree of change were compared to time periods of historical records of cyclones occurring in Toliara Province.
- Full Text:
- Date Issued: 2018
- Authors: Lemahieu, Anne , Scott, Lucy E P , Malherbe, Willem , Mahatante, Paubert T , Randrianarimanana, José V , Aswani, Shankar
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/145370 , vital:38432 , DOI: 10.1016/j.ocecoaman.2018.06.012
- Description: Southwest Madagascar is a region that is significantly impacted upon by climate change. As in a lot of developing countries, the livelihoods of many communities in this region are dependent on fishing. This makes these communities particularly vulnerable to climate-related changes. We conducted a survey in two coastal fishing communities in the Toliara Province, Ambola and Ambotsibotsike. Using a free listing exercise, semi-structured interviews and focus group methods, we documented local perceptions of environmental changes and responses to changes. Results were compared, taking into account the differences in the degree of remoteness, market exposure and religiosity. Time periods that respondents reported as having had a high degree of change were compared to time periods of historical records of cyclones occurring in Toliara Province.
- Full Text:
- Date Issued: 2018
Losing, using, refusing, cruising: first-generation South African women academics narrate the complexity of marginality
- Idahosa, Grace E, Vincent, Louise
- Authors: Idahosa, Grace E , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141820 , vital:38007 , DOI: 10.1080/10130950.2014.874766
- Description: In this article we ask how senior academic women in South Africa narrate their experience of being ‘outside in’ the teaching machine. Wide research literature documents the gross underrepresentation of women in senior positions in the academy. It has been argued that intertwined sexist, patriarchal and phallocentric knowledges and practices in academic institutions produce various forms of discrimination, inequality, oppression and marginalisation. Academic women report feeling invisible and retreating to the margins so as to avoid victimisation and discrimination. Others have pointed to the tension between the ‘tenure clock’ and ‘biological clock’ as a source of anxiety. However, experiences of academic women are not identical. In the context of studies showing the importance of existing personal and social resources, prior experience and having mentors and role models in the negotiation of inequality and discrimination, we document the narratives of women academics who are the first in their families to graduate with a university degree.
- Full Text:
- Date Issued: 2014
- Authors: Idahosa, Grace E , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141820 , vital:38007 , DOI: 10.1080/10130950.2014.874766
- Description: In this article we ask how senior academic women in South Africa narrate their experience of being ‘outside in’ the teaching machine. Wide research literature documents the gross underrepresentation of women in senior positions in the academy. It has been argued that intertwined sexist, patriarchal and phallocentric knowledges and practices in academic institutions produce various forms of discrimination, inequality, oppression and marginalisation. Academic women report feeling invisible and retreating to the margins so as to avoid victimisation and discrimination. Others have pointed to the tension between the ‘tenure clock’ and ‘biological clock’ as a source of anxiety. However, experiences of academic women are not identical. In the context of studies showing the importance of existing personal and social resources, prior experience and having mentors and role models in the negotiation of inequality and discrimination, we document the narratives of women academics who are the first in their families to graduate with a university degree.
- Full Text:
- Date Issued: 2014
Making sense of science through two languages: a South African case study
- Authors: Probyn, Margie J
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:7025 , http://hdl.handle.net/10962/d1007209
- Description: Mzamo Senior Secondary School (not its real name) looks like any typical township school in South Africa: modern face-brick classroom blocks which conceal a serious lack of resources, material and human. Under apartheid the disparities in spending on white and African students meant hugely different teaching and learning contexts - including infrastructure, teacher training, pupil: teacher ratios and teaching materials. Today, 10 years into the new democracy and despite government’s efforts to equalize spending, the historic inequalities persist. The school is surrounded by township houses and shacks that reflect the high poverty levels in the Eastern Cape Province. Students at schools like this face an additional but less obvious problem – that of the language medium. Although in the Eastern Cape, Xhosa is the home language of 83,8% of the population and English speakers comprise only 3.7%, the official language medium in schools is English from the beginning of Grade 4 in the majority of schools. South Africa is a multilingual country with 11 official languages recognized in the Constitution of 1996 – 9 indigenous languages and the two colonial languages, English and Afrikaans.
- Full Text:
- Date Issued: 2004
- Authors: Probyn, Margie J
- Date: 2004
- Language: English
- Type: text , Article
- Identifier: vital:7025 , http://hdl.handle.net/10962/d1007209
- Description: Mzamo Senior Secondary School (not its real name) looks like any typical township school in South Africa: modern face-brick classroom blocks which conceal a serious lack of resources, material and human. Under apartheid the disparities in spending on white and African students meant hugely different teaching and learning contexts - including infrastructure, teacher training, pupil: teacher ratios and teaching materials. Today, 10 years into the new democracy and despite government’s efforts to equalize spending, the historic inequalities persist. The school is surrounded by township houses and shacks that reflect the high poverty levels in the Eastern Cape Province. Students at schools like this face an additional but less obvious problem – that of the language medium. Although in the Eastern Cape, Xhosa is the home language of 83,8% of the population and English speakers comprise only 3.7%, the official language medium in schools is English from the beginning of Grade 4 in the majority of schools. South Africa is a multilingual country with 11 official languages recognized in the Constitution of 1996 – 9 indigenous languages and the two colonial languages, English and Afrikaans.
- Full Text:
- Date Issued: 2004
Management and Commerce Foundation: APR 111 F
- Authors: Siziba, L P , Makwela, B
- Date: 2011-07
- Language: English
- Type: Examination paper
- Identifier: vital:18263 , http://hdl.handle.net/10353/d1011259
- Description: Management and Commerce Foundation: APR 111 F, supplementary examination July 2011.
- Full Text: false
- Date Issued: 2011-07
- Authors: Siziba, L P , Makwela, B
- Date: 2011-07
- Language: English
- Type: Examination paper
- Identifier: vital:18263 , http://hdl.handle.net/10353/d1011259
- Description: Management and Commerce Foundation: APR 111 F, supplementary examination July 2011.
- Full Text: false
- Date Issued: 2011-07
Medicine labels incorporating pictograms: do they influence understanding and adherence?
- Dowse, Roslind, Ehlers, Martina S
- Authors: Dowse, Roslind , Ehlers, Martina S
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6359 , http://hdl.handle.net/10962/d1006051
- Description: The objective was to determine the influence of medicine labels incorporating pictograms on the understanding of instructions and on adherence. Eighty-seven Xhosa participants attending an outpatient clinic who had been prescribed a short course of antibiotics were randomly allocated to either a control group (41 participants given text-only labels), or an experimental group (46 participants given text + pictogram labels). All participants had a maximum of 10 years of formal schooling. Follow-up home visits were conducted after 3–5 days to assess understanding of instructions and to evaluate adherence. A high adherence of greater than 90% was found for 54% of the experimental group, compared with only 2% of the control group. Average percentages for understanding in the control and experimental groups were 70 and 95%, respectively, and average adherence was 72 and 90%, respectively. The presence of pictograms was found to contribute positively to both understanding of instructions and adherence.
- Full Text:
- Date Issued: 2005
- Authors: Dowse, Roslind , Ehlers, Martina S
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6359 , http://hdl.handle.net/10962/d1006051
- Description: The objective was to determine the influence of medicine labels incorporating pictograms on the understanding of instructions and on adherence. Eighty-seven Xhosa participants attending an outpatient clinic who had been prescribed a short course of antibiotics were randomly allocated to either a control group (41 participants given text-only labels), or an experimental group (46 participants given text + pictogram labels). All participants had a maximum of 10 years of formal schooling. Follow-up home visits were conducted after 3–5 days to assess understanding of instructions and to evaluate adherence. A high adherence of greater than 90% was found for 54% of the experimental group, compared with only 2% of the control group. Average percentages for understanding in the control and experimental groups were 70 and 95%, respectively, and average adherence was 72 and 90%, respectively. The presence of pictograms was found to contribute positively to both understanding of instructions and adherence.
- Full Text:
- Date Issued: 2005
Molecular chaperones in biology, medicine and protein biotechnology
- Boshoff, Aileen, Nicoll, William S, Hennessy, Fritha, Ludewig, M H, Daniel, Sheril, Modisakeng, Keoagile W, Shonhai, Addmore, McNamara, Caryn, Bradley, Graeme, Blatch, Gregory L
- Authors: Boshoff, Aileen , Nicoll, William S , Hennessy, Fritha , Ludewig, M H , Daniel, Sheril , Modisakeng, Keoagile W , Shonhai, Addmore , McNamara, Caryn , Bradley, Graeme , Blatch, Gregory L
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6457 , http://hdl.handle.net/10962/d1004479
- Description: Molecular chaperones consist of several highly conserved families of proteins, many of which consist of heat shock proteins. The primary function of molecular chaperones is to facilitate the folding or refolding of proteins, and therefore they play an important role in diverse cellular processes including protein synthesis, protein translocation, and the refolding or degradation of proteins after cell stress. Cells are often exposed to different stressors, resulting in protein misfolding and aggregation. It is now well established that the levels of certain molecular chaperones are elevated during stress to provide protection to the cell. The focus of this review is on the impact of molecular chaperones in biology, medicine and protein biotechnology, and thus covers both fundamental and applied aspects of chaperone biology. Attention is paid to the functions and applications of molecular chaperones from bacterial and eukaryotic cells, focusing on the heat shock proteins 90 (Hsp90), 70 (Hsp70) and 40 (Hsp40) classes of chaperones, respectively. The role of these classes of chaperones in human diseases is discussed, as well as the parts played by chaperones produced by the causative agents of malaria and trypanosomiasis. Recent advances have seen the application of chaperones in improving the yields of a particular target protein in recombinant protein production. The prospects for the targeted use of molecular chaperones for the over-production of recombinant proteins is critically reviewed, and current research on these chaperones at Rhodes University is also discussed.
- Full Text:
- Date Issued: 2004
- Authors: Boshoff, Aileen , Nicoll, William S , Hennessy, Fritha , Ludewig, M H , Daniel, Sheril , Modisakeng, Keoagile W , Shonhai, Addmore , McNamara, Caryn , Bradley, Graeme , Blatch, Gregory L
- Date: 2004
- Language: English
- Type: Article
- Identifier: vital:6457 , http://hdl.handle.net/10962/d1004479
- Description: Molecular chaperones consist of several highly conserved families of proteins, many of which consist of heat shock proteins. The primary function of molecular chaperones is to facilitate the folding or refolding of proteins, and therefore they play an important role in diverse cellular processes including protein synthesis, protein translocation, and the refolding or degradation of proteins after cell stress. Cells are often exposed to different stressors, resulting in protein misfolding and aggregation. It is now well established that the levels of certain molecular chaperones are elevated during stress to provide protection to the cell. The focus of this review is on the impact of molecular chaperones in biology, medicine and protein biotechnology, and thus covers both fundamental and applied aspects of chaperone biology. Attention is paid to the functions and applications of molecular chaperones from bacterial and eukaryotic cells, focusing on the heat shock proteins 90 (Hsp90), 70 (Hsp70) and 40 (Hsp40) classes of chaperones, respectively. The role of these classes of chaperones in human diseases is discussed, as well as the parts played by chaperones produced by the causative agents of malaria and trypanosomiasis. Recent advances have seen the application of chaperones in improving the yields of a particular target protein in recombinant protein production. The prospects for the targeted use of molecular chaperones for the over-production of recombinant proteins is critically reviewed, and current research on these chaperones at Rhodes University is also discussed.
- Full Text:
- Date Issued: 2004
Mulch tower treatment system for greywater reuse Part II: destructive testing and effluent treatment
- Tandlich, Roman, Zuma, Bongumusa M, Whittington-Jones, Kevin J, Burgess, Jo E
- Authors: Tandlich, Roman , Zuma, Bongumusa M , Whittington-Jones, Kevin J , Burgess, Jo E
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71547 , vital:29863 , https://doi.org/10.1016/j.desal.2008.03.031
- Description: The mulch tower (MT) system described in Part I was tested to failure to determine its range of operating conditions. An increase in the influent temperature led to a statistically significant release of components of the chemical oxygen demand (COD) and the five day biochemical oxygen demand (BOD5), as well as phosphates from the MT system. Heterotrophic plate count (HPC) of the mulch layer dropped from 1.2 (± 0.6) × 106/g dry weight to 1.5 (± 0.3) × 105/g dry weight of the mulch layer with increases of the influent temperature. This indicates that the increase in influent temperature killed off some of the active biomass in the MT biofilm. After a five day drying period under active aeration, the MT system retained the ability to remove COD, total suspended solids (TSS), and nitrates. Greywater treatment by the MT system became impossible after a 48 day drying period under active aeration. Chlorination of the simulated MT effluent with a mixture of sodium dichloroisocyanurate and trichloroisocyanuric acid decreased the faecal coliform concentrations (FC) and the total coliform concentrations (TC) below 800 CFUs/100 ml within 65 h. Beyond 65 h, the pH of the effluent became highly acidic. To maintain optimum performance influent should be fed into the MT system at least once every 5 days, sufficient aeration should be guaranteed, and the MT effluent should be chlorinated for 65 h to eliminate all pathogens before any reuse.
- Full Text: false
- Date Issued: 2009
Mulch tower treatment system for greywater reuse Part II: destructive testing and effluent treatment
- Authors: Tandlich, Roman , Zuma, Bongumusa M , Whittington-Jones, Kevin J , Burgess, Jo E
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71547 , vital:29863 , https://doi.org/10.1016/j.desal.2008.03.031
- Description: The mulch tower (MT) system described in Part I was tested to failure to determine its range of operating conditions. An increase in the influent temperature led to a statistically significant release of components of the chemical oxygen demand (COD) and the five day biochemical oxygen demand (BOD5), as well as phosphates from the MT system. Heterotrophic plate count (HPC) of the mulch layer dropped from 1.2 (± 0.6) × 106/g dry weight to 1.5 (± 0.3) × 105/g dry weight of the mulch layer with increases of the influent temperature. This indicates that the increase in influent temperature killed off some of the active biomass in the MT biofilm. After a five day drying period under active aeration, the MT system retained the ability to remove COD, total suspended solids (TSS), and nitrates. Greywater treatment by the MT system became impossible after a 48 day drying period under active aeration. Chlorination of the simulated MT effluent with a mixture of sodium dichloroisocyanurate and trichloroisocyanuric acid decreased the faecal coliform concentrations (FC) and the total coliform concentrations (TC) below 800 CFUs/100 ml within 65 h. Beyond 65 h, the pH of the effluent became highly acidic. To maintain optimum performance influent should be fed into the MT system at least once every 5 days, sufficient aeration should be guaranteed, and the MT effluent should be chlorinated for 65 h to eliminate all pathogens before any reuse.
- Full Text: false
- Date Issued: 2009
Multiple drivers of local (non-) compliance in community-cased marine resource management: case studies from the South Pacific
- Rohe, Janne R, Aswani, Shankar, Schlüter, Achim, Ferse, Sebastian C A
- Authors: Rohe, Janne R , Aswani, Shankar , Schlüter, Achim , Ferse, Sebastian C A
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/70571 , vital:29676 , https://doi.org/10.3389/fmars.2017.00172
- Description: The outcomes of marine conservation and related management interventions depend to a large extent on people's compliance with these rule systems. In the South Pacific, community-based marine resource management (CBMRM) has gained wide recognition as a strategy for the sustainable management of marine resources. In current practice, CBMRM initiatives often build upon customary forms of marine governance, integrating scientific advice and management principles in collaboration with external partners. However, diverse socio-economic developments as well as limited legal mandates can challenge these approaches. Compliance with and effective (legally-backed) enforcement of local management strategies constitute a growing challenge for communities—often resulting in considerable impact on the success or failure of CBMRM. Marine management arrangements are highly dynamic over time, and similarly compliance with rule systems tends to change depending on context. Understanding the factors contributing to (non-) compliance in a given setting is key to the design and function of adaptive management approaches. Yet, few empirical studies have looked in depth into the dynamics around local (non-) compliance with local marine tenure rules under the transforming management arrangements. Using two case studies from Solomon Islands and Fiji, we investigate what drives local (non-) compliance with CBMRM and what hinders or supports its effective enforcement. The case studies reveal that non-compliance is mainly driven by: (1) diminishing perceived legitimacy of local rules and rule-makers; (2) increased incentives to break rules due to market access and/ or lack of alternative income; and (3) relatively weak enforcement of local rules (i.e., low perceptions of risk from sanctions for rule-breaking). These drivers do not stand alone but can act together and add up to impair effective management. We further analyze how enforcement of CBMRM is challenged through a range of institutional; socio-cultural and technical/financial constraints, which are in parts a result of the dynamism and ongoing transformations of management arrangements. Our study underlines the importance of better understanding and contextualizing marine resource management processes under dynamic conditions for an improved understanding of compliance in a particular setting.
- Full Text:
- Date Issued: 2016
- Authors: Rohe, Janne R , Aswani, Shankar , Schlüter, Achim , Ferse, Sebastian C A
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/70571 , vital:29676 , https://doi.org/10.3389/fmars.2017.00172
- Description: The outcomes of marine conservation and related management interventions depend to a large extent on people's compliance with these rule systems. In the South Pacific, community-based marine resource management (CBMRM) has gained wide recognition as a strategy for the sustainable management of marine resources. In current practice, CBMRM initiatives often build upon customary forms of marine governance, integrating scientific advice and management principles in collaboration with external partners. However, diverse socio-economic developments as well as limited legal mandates can challenge these approaches. Compliance with and effective (legally-backed) enforcement of local management strategies constitute a growing challenge for communities—often resulting in considerable impact on the success or failure of CBMRM. Marine management arrangements are highly dynamic over time, and similarly compliance with rule systems tends to change depending on context. Understanding the factors contributing to (non-) compliance in a given setting is key to the design and function of adaptive management approaches. Yet, few empirical studies have looked in depth into the dynamics around local (non-) compliance with local marine tenure rules under the transforming management arrangements. Using two case studies from Solomon Islands and Fiji, we investigate what drives local (non-) compliance with CBMRM and what hinders or supports its effective enforcement. The case studies reveal that non-compliance is mainly driven by: (1) diminishing perceived legitimacy of local rules and rule-makers; (2) increased incentives to break rules due to market access and/ or lack of alternative income; and (3) relatively weak enforcement of local rules (i.e., low perceptions of risk from sanctions for rule-breaking). These drivers do not stand alone but can act together and add up to impair effective management. We further analyze how enforcement of CBMRM is challenged through a range of institutional; socio-cultural and technical/financial constraints, which are in parts a result of the dynamism and ongoing transformations of management arrangements. Our study underlines the importance of better understanding and contextualizing marine resource management processes under dynamic conditions for an improved understanding of compliance in a particular setting.
- Full Text:
- Date Issued: 2016
National Labour and Economic Development Institute Annual report
- NALEDI
- Authors: NALEDI
- Date: 1994
- Subjects: NALEDI
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/151179 , vital:39036
- Description: The National Labour & Economic Development Institute (NALEDI) opened its doors in October 1993 and began its research programme at the beginning of 1994. NALEDI is an initiative of the Congress of South African Trade Unions (Cosatu). Its aim is to conduct policy-relevant research of interest to the labour movement. The origins of the institute date back to Cosatu's 1991 national congress which recognised the need to improve the research capacity of the federation. A feasibility study was conducted. This attempted to define the research needs of the federation and its affiliates. It also examined the experience of other union- linked institutes such as FAFO in Norway and DESEP in Brazil, and explored Cosatu's experience with service organisations and associated research groups. In early 1993 Cosatu's central executive committee endorsed the establishment of a labour research institute and raised funds (primarily from the Dutch union movement the FNV) to commence operations. Jeremy Baskin was appointed to get the institute functioning and a board was appointed to assist.
- Full Text:
- Date Issued: 1994
- Authors: NALEDI
- Date: 1994
- Subjects: NALEDI
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/151179 , vital:39036
- Description: The National Labour & Economic Development Institute (NALEDI) opened its doors in October 1993 and began its research programme at the beginning of 1994. NALEDI is an initiative of the Congress of South African Trade Unions (Cosatu). Its aim is to conduct policy-relevant research of interest to the labour movement. The origins of the institute date back to Cosatu's 1991 national congress which recognised the need to improve the research capacity of the federation. A feasibility study was conducted. This attempted to define the research needs of the federation and its affiliates. It also examined the experience of other union- linked institutes such as FAFO in Norway and DESEP in Brazil, and explored Cosatu's experience with service organisations and associated research groups. In early 1993 Cosatu's central executive committee endorsed the establishment of a labour research institute and raised funds (primarily from the Dutch union movement the FNV) to commence operations. Jeremy Baskin was appointed to get the institute functioning and a board was appointed to assist.
- Full Text:
- Date Issued: 1994
Non-timber forest product use and market chains along a deforestation gradient in southwest Malawi
- Mahonya, Sophie, Shackleton, Charlie M, Schreckenberg, Kate
- Authors: Mahonya, Sophie , Shackleton, Charlie M , Schreckenberg, Kate
- Date: 2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/177465 , vital:42824 , https://doi.org/10.3389/ffgc.2019.00071
- Description: The importance of non-timber forest products (NTFPs) to rural livelihoods is widely acknowledged globally, as is the income generated from casual or fulltime trade on village and urban markets. However, there is less understanding of how the condition or status of the neighboring landscapes influence the use of and trade in NTFPs. Here we report on the use and trade in NTFPs in four villages situated along a gradient of decreasing forest cover in southwest Malawi using a mixed-methods approach. Data were sourced via a survey of 286 households, value chain analysis of the four most commonly traded NTFPs (thatch grass, edible orchids, mushrooms, and wild fruits), key informant interviews with NTFP traders and direct observations.
- Full Text:
- Date Issued: 2019
- Authors: Mahonya, Sophie , Shackleton, Charlie M , Schreckenberg, Kate
- Date: 2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/177465 , vital:42824 , https://doi.org/10.3389/ffgc.2019.00071
- Description: The importance of non-timber forest products (NTFPs) to rural livelihoods is widely acknowledged globally, as is the income generated from casual or fulltime trade on village and urban markets. However, there is less understanding of how the condition or status of the neighboring landscapes influence the use of and trade in NTFPs. Here we report on the use and trade in NTFPs in four villages situated along a gradient of decreasing forest cover in southwest Malawi using a mixed-methods approach. Data were sourced via a survey of 286 households, value chain analysis of the four most commonly traded NTFPs (thatch grass, edible orchids, mushrooms, and wild fruits), key informant interviews with NTFP traders and direct observations.
- Full Text:
- Date Issued: 2019
Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions
- Hennessy, Fritha, Nicoll, Willam S, Zimmerman, Richard, Cheetham, Michael E, Blatch, Gregory L
- Authors: Hennessy, Fritha , Nicoll, Willam S , Zimmerman, Richard , Cheetham, Michael E , Blatch, Gregory L
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6487 , http://hdl.handle.net/10962/d1006270 , http://dx.doi.org/10.1110/ps.051406805
- Description: Heat shock protein 40s (Hsp40s) and heat shock protein 70s (Hsp70s) form chaperone partnerships that are key components of cellular chaperone networks involved in facilitating the correct folding of a broad range of client proteins. While the Hsp40 family of proteins is highly diverse with multiple forms occurring in any particular cell or compartment, all its members are characterized by a J domain that directs their interaction with a partner Hsp70. Specific Hsp40-Hsp70 chaperone partnerships have been identified that are dedicated to the correct folding of distinct subsets of client proteins. The elucidation of the mechanism by which these specific Hsp40-Hsp70 partnerships are formed will greatly enhance our understanding of the way in which chaperone pathways are integrated into finely regulated protein folding networks. From in silico analyses, domain swapping and rational protein engineering experiments, evidence has accumulated that indicates that J domains contain key specificity determinants. This review will critically discuss the current understanding of the structural features of J domains that determine the specificity of interaction between Hsp40 proteins and their partner Hsp70s. We also propose a model in which the J domain is able to integrate specificity and chaperone activity.
- Full Text:
- Date Issued: 2005
- Authors: Hennessy, Fritha , Nicoll, Willam S , Zimmerman, Richard , Cheetham, Michael E , Blatch, Gregory L
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6487 , http://hdl.handle.net/10962/d1006270 , http://dx.doi.org/10.1110/ps.051406805
- Description: Heat shock protein 40s (Hsp40s) and heat shock protein 70s (Hsp70s) form chaperone partnerships that are key components of cellular chaperone networks involved in facilitating the correct folding of a broad range of client proteins. While the Hsp40 family of proteins is highly diverse with multiple forms occurring in any particular cell or compartment, all its members are characterized by a J domain that directs their interaction with a partner Hsp70. Specific Hsp40-Hsp70 chaperone partnerships have been identified that are dedicated to the correct folding of distinct subsets of client proteins. The elucidation of the mechanism by which these specific Hsp40-Hsp70 partnerships are formed will greatly enhance our understanding of the way in which chaperone pathways are integrated into finely regulated protein folding networks. From in silico analyses, domain swapping and rational protein engineering experiments, evidence has accumulated that indicates that J domains contain key specificity determinants. This review will critically discuss the current understanding of the structural features of J domains that determine the specificity of interaction between Hsp40 proteins and their partner Hsp70s. We also propose a model in which the J domain is able to integrate specificity and chaperone activity.
- Full Text:
- Date Issued: 2005
Nutrient release dynamics associated with native and invasive leaf litter decomposition: a Mesocosm Experiment
- Mutshekwa, Thendo, Cuthbert, Ross N, Wasserman, Ryan J, Murungweni, Florence M, Dalu, Tatenda
- Authors: Mutshekwa, Thendo , Cuthbert, Ross N , Wasserman, Ryan J , Murungweni, Florence M , Dalu, Tatenda
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/160467 , vital:40448 , https://doi.org/10.3390/w12092350
- Description: Leaf litter contributes to the functioning of aquatic ecosystems through allochthonous inputs of carbon, nitrogen, and other elements. Here, we examine leaf litter nutrient inputs and decomposition associated with four plant species using a mesocosm approach. Native sycamore fig Ficus sycomorus L., and silver cluster–leaf Terminalia sericea Burch. ex DC. decomposition dynamics were compared to invasive tickberry Lantana camara L. and guava Psidium guajava L., whereby phosphate, nitrate, nitrite, silicate, and ammonium releases were quantified over time. Leaf inputs significantly reduced pH, with reductions most marked by invasive L. camara. Conductivity was heightened by all leaf input treatments, except native T. sericea.
- Full Text:
- Date Issued: 2020
- Authors: Mutshekwa, Thendo , Cuthbert, Ross N , Wasserman, Ryan J , Murungweni, Florence M , Dalu, Tatenda
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/160467 , vital:40448 , https://doi.org/10.3390/w12092350
- Description: Leaf litter contributes to the functioning of aquatic ecosystems through allochthonous inputs of carbon, nitrogen, and other elements. Here, we examine leaf litter nutrient inputs and decomposition associated with four plant species using a mesocosm approach. Native sycamore fig Ficus sycomorus L., and silver cluster–leaf Terminalia sericea Burch. ex DC. decomposition dynamics were compared to invasive tickberry Lantana camara L. and guava Psidium guajava L., whereby phosphate, nitrate, nitrite, silicate, and ammonium releases were quantified over time. Leaf inputs significantly reduced pH, with reductions most marked by invasive L. camara. Conductivity was heightened by all leaf input treatments, except native T. sericea.
- Full Text:
- Date Issued: 2020
Occurrence of selected endocrine disrupting compounds in the Eastern Cape province of South Africa:
- Farounbi, Adebayo I, Ngqwala, Nosiphiwe P
- Authors: Farounbi, Adebayo I , Ngqwala, Nosiphiwe P
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148447 , vital:38740 , DOI: 10.1007/s11356-020-08082-y
- Description: Endocrine-disrupting compounds are attracting attention worldwide because of their effects on living things in the environment. Ten endocrine disrupting compounds: 4-nonylphenol, 2,4-dichlorophenol, estrone, 17β-estradiol, bisphenol A, 4-tertoctylphenol, triclosan, atrazine, imidazole and 1,2,4-triazole were investigated in four rivers and wastewater treatment plants in this study. Rivers were sampled at upstream, midstream and downstream reaches, while the influent and effluent samples of wastewater were collected from treatment plants near the receiving rivers. Sample waters were freeze-dried followed by extraction of the organic content and purification by solid-phase extraction. Concentrations of the compounds in the samples were determined with ultra-high performance liquid chromatography-tandem mass spectrometry. The instrument was operated in the positive electrospray ionization (ESI) mode.
- Full Text:
- Date Issued: 2020
- Authors: Farounbi, Adebayo I , Ngqwala, Nosiphiwe P
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148447 , vital:38740 , DOI: 10.1007/s11356-020-08082-y
- Description: Endocrine-disrupting compounds are attracting attention worldwide because of their effects on living things in the environment. Ten endocrine disrupting compounds: 4-nonylphenol, 2,4-dichlorophenol, estrone, 17β-estradiol, bisphenol A, 4-tertoctylphenol, triclosan, atrazine, imidazole and 1,2,4-triazole were investigated in four rivers and wastewater treatment plants in this study. Rivers were sampled at upstream, midstream and downstream reaches, while the influent and effluent samples of wastewater were collected from treatment plants near the receiving rivers. Sample waters were freeze-dried followed by extraction of the organic content and purification by solid-phase extraction. Concentrations of the compounds in the samples were determined with ultra-high performance liquid chromatography-tandem mass spectrometry. The instrument was operated in the positive electrospray ionization (ESI) mode.
- Full Text:
- Date Issued: 2020
Ocean warming, a rapid distributional shift, and the hybridization of a coastal fish species
- Potts, Warren M, Henriques, Romina, Santos, Carmen V D, Munnik, Kate, Ansorge, Isabelle J, Dufois, Francois, Sauer, Warwick H H, Booth, Anthony J, Kirchner, Carola, Sauer, Warwick, Shaw, Paul W
- Authors: Potts, Warren M , Henriques, Romina , Santos, Carmen V D , Munnik, Kate , Ansorge, Isabelle J , Dufois, Francois , Sauer, Warwick H H , Booth, Anthony J , Kirchner, Carola , Sauer, Warwick , Shaw, Paul W
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125375 , vital:35777 , https://doi.10.1111/gcb.12612
- Description: Despite increasing awareness of large-scale climate-driven distribution shifts in the marine environment, no study has linked rapid ocean warming to a shift in distribution and consequent hybridization of a marine fish species. This study describes rapid warming (0.8 °C per decade) in the coastal waters of the Angola-Benguela Frontal Zone over the last three decades and a concomitant shift by a temperature sensitive coastal fish species (Argyrosomus coronus) southward from Angola into Namibia. In this context, rapid shifts in distribution across Economic Exclusive Zones will complicate the management of fishes, particularly when there is a lack of congruence in the fisheries policy between nations. Evidence for recent hybridization between A. coronus and a congener, A. inodorus, indicate that the rapid shift in distribution of A. coronus has placed adults of the two species in contact during their spawning events. Ocean warming may therefore revert established species isolation mechanisms and alter the evolutionary history of fishes. While the consequences of the hybridization on the production of the resource remain unclear, this will most likely introduce additional layers of complexity to their management.
- Full Text:
- Date Issued: 2014
- Authors: Potts, Warren M , Henriques, Romina , Santos, Carmen V D , Munnik, Kate , Ansorge, Isabelle J , Dufois, Francois , Sauer, Warwick H H , Booth, Anthony J , Kirchner, Carola , Sauer, Warwick , Shaw, Paul W
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125375 , vital:35777 , https://doi.10.1111/gcb.12612
- Description: Despite increasing awareness of large-scale climate-driven distribution shifts in the marine environment, no study has linked rapid ocean warming to a shift in distribution and consequent hybridization of a marine fish species. This study describes rapid warming (0.8 °C per decade) in the coastal waters of the Angola-Benguela Frontal Zone over the last three decades and a concomitant shift by a temperature sensitive coastal fish species (Argyrosomus coronus) southward from Angola into Namibia. In this context, rapid shifts in distribution across Economic Exclusive Zones will complicate the management of fishes, particularly when there is a lack of congruence in the fisheries policy between nations. Evidence for recent hybridization between A. coronus and a congener, A. inodorus, indicate that the rapid shift in distribution of A. coronus has placed adults of the two species in contact during their spawning events. Ocean warming may therefore revert established species isolation mechanisms and alter the evolutionary history of fishes. While the consequences of the hybridization on the production of the resource remain unclear, this will most likely introduce additional layers of complexity to their management.
- Full Text:
- Date Issued: 2014
Olive Schreiner at 150: some thoughts on re-editing Cronwright's The Reinterment on Buffelskop
- Walters, Paul S, Fogg, W Jeremy M
- Authors: Walters, Paul S , Fogg, W Jeremy M
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:6124 , http://hdl.handle.net/10962/d1004708
- Description: [From the introduction]: The original edition of Cronwright’s The Reinterment on Buffelskop (1983) was produced by Guy Butler and Nick Visser to commemorate the centenary of the 1883 publication of The Story of an African Farm. The Butler-Visser text was a photographic reproduction of a typed carbon copy of the first part of Cronwright’s extant diaries plus a special diary he had kept covering in detail the events of the actual reinterment. (The originals are now at the National English Literary Museum [NELM].) Butler included a comprehensive and illuminating introduction to these texts, as well as – under separate soft cover – a set of “Provisional Notes” which draw deeply on his own and his family’s accumulated knowledge of Cradock, its environs and inhabitants. In addition, Butler and Visser included two passages excised by Cronwright from the typescript of his Life of Olive Schreiner: a word picture of Charles Heathcote, and the longer account of “The Nienaber Incident” – pages which deal with the execution of three innocent men at De Aar on 19 March 1901, and Cronwright’s subsequent attempts at legal reparation for them and their families. The substantive text of the Butler-Visser edition is often difficult to read because of the method of reproduction; moreover, because it also reproduces Cronwright’s emendations (in ink) of the typescript, it is frankly uninviting. Thus, when the NELM Council proposed a publication commemorating the 150th anniversary of Olive Schreiner’s birth on 24 March 1855, it seemed appropriate that a second attempt be made to give students of Olive Schreiner’s works easier access to Cronwright’s detailed account of this “bizarre, romantic” episode. Furthermore, from the perspective of text history, the typescript of the Reinterment antedates both Cronwright’s Life and The Letters of Olive Schreiner. Parts of it are clearly Cronwright’s preliminary ‘notes towards’ his Life, and, as Butler hypothesizes, the whole of the Reinterment might have been intended as a separate (and earlier) publication. Finally, the sarcophagus on Buffelskop is one of South Africa’s more noteworthy literary shrines: while the idea of re-editing an account of Olive Schreiner’s reinterment might be thought to be a futile exercise in intellectual recycling, our intention is that both husband and wife should live again through a rediscovery of the thoughts and feelings that led them to this dramatic final resting-place.
- Full Text:
- Date Issued: 2006
- Authors: Walters, Paul S , Fogg, W Jeremy M
- Date: 2006
- Language: English
- Type: text , Article
- Identifier: vital:6124 , http://hdl.handle.net/10962/d1004708
- Description: [From the introduction]: The original edition of Cronwright’s The Reinterment on Buffelskop (1983) was produced by Guy Butler and Nick Visser to commemorate the centenary of the 1883 publication of The Story of an African Farm. The Butler-Visser text was a photographic reproduction of a typed carbon copy of the first part of Cronwright’s extant diaries plus a special diary he had kept covering in detail the events of the actual reinterment. (The originals are now at the National English Literary Museum [NELM].) Butler included a comprehensive and illuminating introduction to these texts, as well as – under separate soft cover – a set of “Provisional Notes” which draw deeply on his own and his family’s accumulated knowledge of Cradock, its environs and inhabitants. In addition, Butler and Visser included two passages excised by Cronwright from the typescript of his Life of Olive Schreiner: a word picture of Charles Heathcote, and the longer account of “The Nienaber Incident” – pages which deal with the execution of three innocent men at De Aar on 19 March 1901, and Cronwright’s subsequent attempts at legal reparation for them and their families. The substantive text of the Butler-Visser edition is often difficult to read because of the method of reproduction; moreover, because it also reproduces Cronwright’s emendations (in ink) of the typescript, it is frankly uninviting. Thus, when the NELM Council proposed a publication commemorating the 150th anniversary of Olive Schreiner’s birth on 24 March 1855, it seemed appropriate that a second attempt be made to give students of Olive Schreiner’s works easier access to Cronwright’s detailed account of this “bizarre, romantic” episode. Furthermore, from the perspective of text history, the typescript of the Reinterment antedates both Cronwright’s Life and The Letters of Olive Schreiner. Parts of it are clearly Cronwright’s preliminary ‘notes towards’ his Life, and, as Butler hypothesizes, the whole of the Reinterment might have been intended as a separate (and earlier) publication. Finally, the sarcophagus on Buffelskop is one of South Africa’s more noteworthy literary shrines: while the idea of re-editing an account of Olive Schreiner’s reinterment might be thought to be a futile exercise in intellectual recycling, our intention is that both husband and wife should live again through a rediscovery of the thoughts and feelings that led them to this dramatic final resting-place.
- Full Text:
- Date Issued: 2006
On location: Narratives of the South African city of the late 1940s and 1950s in film and literature
- Authors: Baines, Gary F
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125397 , vital:35779 , https://doi.10.1080/02582470308671922
- Description: This article is about narratives, about the forms and meanings constructed by South African storytellers, especially writers and filmmakers. It examines the relationships between examples of these two different narrative forms of literature (fiction and non-fiction) and feature film. Following Turner, the point of departure is that the study of narrative has the potential to provide a framework within which such a two pronged approach can be undertaken. This is not to say that the production of meaning takes place within an exclusive literary or cinematic context. Rather, this approach will allow us to obtain a fuller picture of the narrative of the South African city than is possible by concentrating on one medium. It is based on the premise that narratives are ultimately produced by culture; thus these cultural constructions generate meanings, take on a significance, and assume forms that are articufations of the values, beliefs -the ideology - of the culture.' Literature and film offer specific forms for such narratives. By adopting an interdisciplinary approach which borrows insights from literary and film studies, this article attempts to make a contribution to the fledgling field of South African cultural studies.
- Full Text:
- Date Issued: 2009
On location: Narratives of the South African city of the late 1940s and 1950s in film and literature
- Authors: Baines, Gary F
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125397 , vital:35779 , https://doi.10.1080/02582470308671922
- Description: This article is about narratives, about the forms and meanings constructed by South African storytellers, especially writers and filmmakers. It examines the relationships between examples of these two different narrative forms of literature (fiction and non-fiction) and feature film. Following Turner, the point of departure is that the study of narrative has the potential to provide a framework within which such a two pronged approach can be undertaken. This is not to say that the production of meaning takes place within an exclusive literary or cinematic context. Rather, this approach will allow us to obtain a fuller picture of the narrative of the South African city than is possible by concentrating on one medium. It is based on the premise that narratives are ultimately produced by culture; thus these cultural constructions generate meanings, take on a significance, and assume forms that are articufations of the values, beliefs -the ideology - of the culture.' Literature and film offer specific forms for such narratives. By adopting an interdisciplinary approach which borrows insights from literary and film studies, this article attempts to make a contribution to the fledgling field of South African cultural studies.
- Full Text:
- Date Issued: 2009
Optical limiting and singlet oxygen generation properties of phosphorus triazatetrabenzcorroles
- Mkhize, Colin, Britton, Jonathan, Mack, John, Nyokong, Tebello
- Authors: Mkhize, Colin , Britton, Jonathan , Mack, John , Nyokong, Tebello
- Date: 2015
- Language: English
- Type: Article
- Identifier: vital:7257 , http://hdl.handle.net/10962/d1020266
- Description: Novel phosphorus triazatetrabenzcorrole (TBC) tetrasubstituted at the α- and β-positions of the peripheral fused benzene rings with t-butylphenoxy substituents have been prepared and characterized. The effect of the substituents on the electronic structures and optical properties is investigated with TD-DFT calculations and MCD spectroscopy. The optical limiting properties have been investigated to examine whether the lower symmetry that results from the direct pyrrole–pyrrole bond and hence the permanent dipole moment that is introduced result in higher safety thresholds, relative to the values that have been reported for phthalocyanines. The suitability of the compounds for singlet oxygen applications has also been examined. , Original publication is available at http://dx.doi.org/10.1142/S1088424614501065
- Full Text: false
- Date Issued: 2015
- Authors: Mkhize, Colin , Britton, Jonathan , Mack, John , Nyokong, Tebello
- Date: 2015
- Language: English
- Type: Article
- Identifier: vital:7257 , http://hdl.handle.net/10962/d1020266
- Description: Novel phosphorus triazatetrabenzcorrole (TBC) tetrasubstituted at the α- and β-positions of the peripheral fused benzene rings with t-butylphenoxy substituents have been prepared and characterized. The effect of the substituents on the electronic structures and optical properties is investigated with TD-DFT calculations and MCD spectroscopy. The optical limiting properties have been investigated to examine whether the lower symmetry that results from the direct pyrrole–pyrrole bond and hence the permanent dipole moment that is introduced result in higher safety thresholds, relative to the values that have been reported for phthalocyanines. The suitability of the compounds for singlet oxygen applications has also been examined. , Original publication is available at http://dx.doi.org/10.1142/S1088424614501065
- Full Text: false
- Date Issued: 2015
Optimising design and effort for environmental surveys using dung beetles (Coleoptera: Scarabaeidae):
- Tocco, Claudia, Quinn, Danielle, Midgley, John M, Villet, Martin H
- Authors: Tocco, Claudia , Quinn, Danielle , Midgley, John M , Villet, Martin H
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/140670 , vital:37908 , DOI: 10.4039/tce.2016.48
- Description: In biological monitoring, deploying an effective standardised quantitative sampling method, optimised by trap design and sampling effort, is an essential consideration. To exemplify this using dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae and Aphodiinae) communities, three pitfall trap designs (un-baited (TN), baited at ground level (flat trap, TF), and baited above the trap (hanging trap, TH)), employed with varying levels of sampling effort (number of traps = 1, 2, 3… 10; number of days = 1, 2, 3), were evaluated for sampling completeness and efficiency in the Eastern Cape, South Africa. Modelling and resampling simulation approaches were used to suggest optimal sampling protocols across environmentally diverse sites. Overall, TF recovered the greatest abundance and species richness of dung beetles, but behavioural guilds showed conflicting trends: endocoprids preferred TH while paracoprids and telocoprids preferred TF. Resampling simulation of trap type and the two components of sampling effort suggested that six TF traps left for three days was most efficient in obtaining a representative sample and allowed differentiation between trap types, allowing the improved efficiency to be recognised. The effect of trap type on non-target specimens, particularly ants, was also investigated. TF and TH caught almost no by-catch, which is ethically desirable.
- Full Text:
- Date Issued: 2016
- Authors: Tocco, Claudia , Quinn, Danielle , Midgley, John M , Villet, Martin H
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/140670 , vital:37908 , DOI: 10.4039/tce.2016.48
- Description: In biological monitoring, deploying an effective standardised quantitative sampling method, optimised by trap design and sampling effort, is an essential consideration. To exemplify this using dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae and Aphodiinae) communities, three pitfall trap designs (un-baited (TN), baited at ground level (flat trap, TF), and baited above the trap (hanging trap, TH)), employed with varying levels of sampling effort (number of traps = 1, 2, 3… 10; number of days = 1, 2, 3), were evaluated for sampling completeness and efficiency in the Eastern Cape, South Africa. Modelling and resampling simulation approaches were used to suggest optimal sampling protocols across environmentally diverse sites. Overall, TF recovered the greatest abundance and species richness of dung beetles, but behavioural guilds showed conflicting trends: endocoprids preferred TH while paracoprids and telocoprids preferred TF. Resampling simulation of trap type and the two components of sampling effort suggested that six TF traps left for three days was most efficient in obtaining a representative sample and allowed differentiation between trap types, allowing the improved efficiency to be recognised. The effect of trap type on non-target specimens, particularly ants, was also investigated. TF and TH caught almost no by-catch, which is ethically desirable.
- Full Text:
- Date Issued: 2016
Organiser update: Food & Allied Workers Union Information & Research Department: number 3, vol. 1
- Food and Allied Workers Union
- Authors: Food and Allied Workers Union
- Date: 1990-06
- Subjects: Uncatalogued
- Language: English
- Type: text , pamphlet
- Identifier: http://hdl.handle.net/10962/112426 , vital:33579
- Description: Industrial and mining group Barlow Rand was again selected the SA's top company in the annual Financial Mail Top 100 companies survey. Barlows received top position in rating in the rankings of total assets, sales, profit and market capital at the Johannesburg Stock Exchange. Barlows Received net profit of Rl,06 billion in the year to September 1989 from the sales of R26,4 billion. Ranked by sales CG Smith received the second position, SAB came third and CG Smith Food on the fourth position. Following is the ranking of the too ten companies on the JSE, ranked by sales, net profit and assets.
- Full Text:
- Date Issued: 1990-06
- Authors: Food and Allied Workers Union
- Date: 1990-06
- Subjects: Uncatalogued
- Language: English
- Type: text , pamphlet
- Identifier: http://hdl.handle.net/10962/112426 , vital:33579
- Description: Industrial and mining group Barlow Rand was again selected the SA's top company in the annual Financial Mail Top 100 companies survey. Barlows received top position in rating in the rankings of total assets, sales, profit and market capital at the Johannesburg Stock Exchange. Barlows Received net profit of Rl,06 billion in the year to September 1989 from the sales of R26,4 billion. Ranked by sales CG Smith received the second position, SAB came third and CG Smith Food on the fourth position. Following is the ranking of the too ten companies on the JSE, ranked by sales, net profit and assets.
- Full Text:
- Date Issued: 1990-06