Over-expression, purification and biochemical characterisation of trypanosomal heat shock protein
- Authors: Edkins, Adrienne Lesley
- Date: 2003
- Subjects: Heat shock proteins , Heat shock proteins -- Structure activity relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4106 , http://hdl.handle.net/10962/d1011736 , Heat shock proteins , Heat shock proteins -- Structure activity relationships
- Description: The molecular chaperone process of assisted protein folding, characteristic of members of the Heat Shock Protein 70 kDa (Hsp70) and Heat Shock Protein 40kDa (Hsp40) families, is essential for cytoprotection in stressful cellular conditions. Examples of such conditions are heat shock or invasion by pathogens. The Hsp70/Hsp40 process of assisted protein folding is dependent on ATP (governed by the intrinsic ATPase activity of Hsp70) and the ability of molecular chaperones to recognise and bind non-native protein conformations. Here, we analyse and attempt to characterise the molecular chaperone activity of an inducible, cytoplasmic Hsp70 (TcHsp70) from Trypanosoma cruzi and its interactions with its potential partner Hsp40s, Tcj 1, Tcj2, Tcj3 and Tcj4. A bioinformatic analyses of the primary sequences of the trypanosomal proteins revealed that they all contained the canonical domains that define other members of the Hsp70 and Hsp40 family. Tcj2 and Tcj4 showed deviations from the consensus sequence in their substrate binding regions, which may have implications for their substrate binding specificities. TcHsp70, Tcj 1, Tcj2, Tcj3 and Tcj4 were over-expressed recombinantly as 6xHis-tag fusion proteins in Escherichia coli. His-TcHsp70, Tcjl-His and His-Tcj2 were successfully purified by Nickel-affinity chromatography for functional analyses to assess the molecular chaperone activity of His-TcHsp70 in terms of its ATPase activity and substrate binding ability. The basal ATPase activity of His-TcHsp70 was determined as 40 nmol Pi/min/mg, significantly higher than that reported for other Hsp70s. This basal ATPase activity was stimulated to a maximal level of 60 nmol Pi/min/mg in the presence of His-Tcj2 and a model non-native substrate, reduced carboxymethylated αx-lactalbumin (RCMLA). Using native polyacrylamide gel electrophoresis and Western analysis, His-TcHsp70 was shown to form discrete complexes when in the presence of Tcj 1- His, His-Tcj2 and/or RCMLA. These complexes potentially represent His-TcHsp70 - RCMLA or His-TcHsp70 - Tcj interactions, that may be indicative of chaperone activity. In vivo complementation assays showed that Tcj2, but not Tcj3, was able to overcome the temperature sensitivity of the ydjJ mutant Saccharomyces cerevisiae strain JJ160, suggesting that Tcj2 may be functionally equivalent to the yeast Hsp40 Ydj1.
- Full Text:
- Date Issued: 2003
- Authors: Edkins, Adrienne Lesley
- Date: 2003
- Subjects: Heat shock proteins , Heat shock proteins -- Structure activity relationships
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4106 , http://hdl.handle.net/10962/d1011736 , Heat shock proteins , Heat shock proteins -- Structure activity relationships
- Description: The molecular chaperone process of assisted protein folding, characteristic of members of the Heat Shock Protein 70 kDa (Hsp70) and Heat Shock Protein 40kDa (Hsp40) families, is essential for cytoprotection in stressful cellular conditions. Examples of such conditions are heat shock or invasion by pathogens. The Hsp70/Hsp40 process of assisted protein folding is dependent on ATP (governed by the intrinsic ATPase activity of Hsp70) and the ability of molecular chaperones to recognise and bind non-native protein conformations. Here, we analyse and attempt to characterise the molecular chaperone activity of an inducible, cytoplasmic Hsp70 (TcHsp70) from Trypanosoma cruzi and its interactions with its potential partner Hsp40s, Tcj 1, Tcj2, Tcj3 and Tcj4. A bioinformatic analyses of the primary sequences of the trypanosomal proteins revealed that they all contained the canonical domains that define other members of the Hsp70 and Hsp40 family. Tcj2 and Tcj4 showed deviations from the consensus sequence in their substrate binding regions, which may have implications for their substrate binding specificities. TcHsp70, Tcj 1, Tcj2, Tcj3 and Tcj4 were over-expressed recombinantly as 6xHis-tag fusion proteins in Escherichia coli. His-TcHsp70, Tcjl-His and His-Tcj2 were successfully purified by Nickel-affinity chromatography for functional analyses to assess the molecular chaperone activity of His-TcHsp70 in terms of its ATPase activity and substrate binding ability. The basal ATPase activity of His-TcHsp70 was determined as 40 nmol Pi/min/mg, significantly higher than that reported for other Hsp70s. This basal ATPase activity was stimulated to a maximal level of 60 nmol Pi/min/mg in the presence of His-Tcj2 and a model non-native substrate, reduced carboxymethylated αx-lactalbumin (RCMLA). Using native polyacrylamide gel electrophoresis and Western analysis, His-TcHsp70 was shown to form discrete complexes when in the presence of Tcj 1- His, His-Tcj2 and/or RCMLA. These complexes potentially represent His-TcHsp70 - RCMLA or His-TcHsp70 - Tcj interactions, that may be indicative of chaperone activity. In vivo complementation assays showed that Tcj2, but not Tcj3, was able to overcome the temperature sensitivity of the ydjJ mutant Saccharomyces cerevisiae strain JJ160, suggesting that Tcj2 may be functionally equivalent to the yeast Hsp40 Ydj1.
- Full Text:
- Date Issued: 2003
Mythic and theoretic aspects of the concept of 'the unconscious' in popular and psychological discourse
- Authors: Edwards, D J A
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6227 , http://hdl.handle.net/10962/d1007777
- Description: From the introduction: In Greek mythology, Typhon was the youngest son of Gaea (the Earth) and Tartarus (the underworld). Typhon was not a beautiful baby. He was a grisly monster with a hundred dragons' heads. He was one of the Titans, a group of powerful and dangerous creatures who rebelled against Zeus, the King of the Gods. The rebellion was crushed and Typhon was imprisoned under Mount Etna, the volcano in Sicily which was active in classical times and remains active today. It was said that when Typhon raged, the earth shook and Etna erupted. Many such tales from mythology from all over world seem to dramatize aspects of our relationship with potent forces of which we have little understanding and over which we have little control. Many of these forces are less concrete than the forces of nature. They arise from our apprehension of our existential predicaments, our interpersonal vulnerability and the intensity of our own psychological pain. In many contemporary discourses this territory is referred to more neutrally as ‘the unconscious;’ but the unconscious will always elude our attempts to capture it in words.
- Full Text:
- Date Issued: 2003
- Authors: Edwards, D J A
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6227 , http://hdl.handle.net/10962/d1007777
- Description: From the introduction: In Greek mythology, Typhon was the youngest son of Gaea (the Earth) and Tartarus (the underworld). Typhon was not a beautiful baby. He was a grisly monster with a hundred dragons' heads. He was one of the Titans, a group of powerful and dangerous creatures who rebelled against Zeus, the King of the Gods. The rebellion was crushed and Typhon was imprisoned under Mount Etna, the volcano in Sicily which was active in classical times and remains active today. It was said that when Typhon raged, the earth shook and Etna erupted. Many such tales from mythology from all over world seem to dramatize aspects of our relationship with potent forces of which we have little understanding and over which we have little control. Many of these forces are less concrete than the forces of nature. They arise from our apprehension of our existential predicaments, our interpersonal vulnerability and the intensity of our own psychological pain. In many contemporary discourses this territory is referred to more neutrally as ‘the unconscious;’ but the unconscious will always elude our attempts to capture it in words.
- Full Text:
- Date Issued: 2003
Cognitive therapy for social phobia : the human face of cognitive science
- Edwards, David J A, Henwood, Jennifer, Kannan, Swetha
- Authors: Edwards, David J A , Henwood, Jennifer , Kannan, Swetha
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6280 , http://hdl.handle.net/10962/d1008281
- Description: [abstract from Index to SA Periodicals]Points to the male/masculine ideology pervading science. Gives a history of cognitive science. Shows that current clinical models on which cognitive therapy treatments are based are complex and detailed, but also situated and human. Warns about the contemporary enthusiasm for cognitive science. Presents a case study which illustrates how the cognitive model of social phobia works inpractice when applied to one person's life situation.
- Full Text:
- Date Issued: 2003
- Authors: Edwards, David J A , Henwood, Jennifer , Kannan, Swetha
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6280 , http://hdl.handle.net/10962/d1008281
- Description: [abstract from Index to SA Periodicals]Points to the male/masculine ideology pervading science. Gives a history of cognitive science. Shows that current clinical models on which cognitive therapy treatments are based are complex and detailed, but also situated and human. Warns about the contemporary enthusiasm for cognitive science. Presents a case study which illustrates how the cognitive model of social phobia works inpractice when applied to one person's life situation.
- Full Text:
- Date Issued: 2003
Disturbances of attitudes and behaviours related to eating in black and white females at high school and university in South Africa
- Edwards, David J A, d'Agrela, A, Geach, M, Welman, Mark
- Authors: Edwards, David J A , d'Agrela, A , Geach, M , Welman, Mark
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6246 , http://hdl.handle.net/10962/d1007864
- Description: This paper reports two studies, which contribute to the increasing evidence that the attitudes and behaviours associated with eating disorders, are encountered among both black and white females in South Africa. In Study One, the Eating Disorders Inventory EDI was administered to black (n=39) and white (n=41) female students in Natal. There were no significant differences between black and white on the sub-scales which measure disturbed eating behaviour directly (Drive for Thinness, Bulimia, Body Dissatisfaction). However black respondents scrored higher on Perfectionism, Interpersonal Distrust and Maturity Fears, variables believed to predispose individuals to eating disorders. In Study Two, the Bulimia Test (BULIT) was administered to black and white females at three educational levels. There was no significant effect of Ethnicity, but there was a significant effect of Age: Standard 6 respondents had significantly higher scores than University students. In both studies, Body mass index (BMI) was significantly higher among blacks than whites. In Study One there was no significant correlation between BMI and Drive for Thinness in either blacks or whites. However in Study Two, the correlation between BMI and BULIT full scale was significant in the case of both blacks (r = 0,39; p <,01) and whites (r = 0,38; p<,05). These findings are consistent with those of other recent studies, which find disturbances in eating-related attitudes and behaviour in all ethnic groups in South Africa.
- Full Text:
- Date Issued: 2003
- Authors: Edwards, David J A , d'Agrela, A , Geach, M , Welman, Mark
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6246 , http://hdl.handle.net/10962/d1007864
- Description: This paper reports two studies, which contribute to the increasing evidence that the attitudes and behaviours associated with eating disorders, are encountered among both black and white females in South Africa. In Study One, the Eating Disorders Inventory EDI was administered to black (n=39) and white (n=41) female students in Natal. There were no significant differences between black and white on the sub-scales which measure disturbed eating behaviour directly (Drive for Thinness, Bulimia, Body Dissatisfaction). However black respondents scrored higher on Perfectionism, Interpersonal Distrust and Maturity Fears, variables believed to predispose individuals to eating disorders. In Study Two, the Bulimia Test (BULIT) was administered to black and white females at three educational levels. There was no significant effect of Ethnicity, but there was a significant effect of Age: Standard 6 respondents had significantly higher scores than University students. In both studies, Body mass index (BMI) was significantly higher among blacks than whites. In Study One there was no significant correlation between BMI and Drive for Thinness in either blacks or whites. However in Study Two, the correlation between BMI and BULIT full scale was significant in the case of both blacks (r = 0,39; p <,01) and whites (r = 0,38; p<,05). These findings are consistent with those of other recent studies, which find disturbances in eating-related attitudes and behaviour in all ethnic groups in South Africa.
- Full Text:
- Date Issued: 2003
The social construction of "sexual knowledge": exploring the narratives of southern African youth of Indian descent in the context of HIV/AIDS
- Authors: Esat, Fazila
- Date: 2003
- Subjects: East Indians -- South Africa -- Attitudes , Youth -- South Africa -- Attitudes , East Indians -- South Africa -- Sexual behavior , Youth -- South Africa -- Sexual behavior , AIDS (Disease) -- Social aspects -- South Africa , HIV infections -- Social aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2970 , http://hdl.handle.net/10962/d1002479 , East Indians -- South Africa -- Attitudes , Youth -- South Africa -- Attitudes , East Indians -- South Africa -- Sexual behavior , Youth -- South Africa -- Sexual behavior , AIDS (Disease) -- Social aspects -- South Africa , HIV infections -- Social aspects -- South Africa
- Description: This study pays attention to youth of Indian-descent within the context of sexuality and identity and their role in HIV/AIDS. By gaining an understanding of this interaction between identity and sexuality, it adds to our knowledge of the social dynamics that contribute to the prevalence or lack of prevalence of HIV/AIDS within population groups. This study uses a social constructionist discourse analytic framework and aims to explore the construction of sexual knowledge by Southern African youth of Indian-descent. The findings indicate that the construction of sex is primarily one of risk and ambiguity. Additionally, the construction of sexual knowledge highlights the significance of gender differentials and the importance of agency and responsibility for sexual education. These constructions reinforce traditional educational roles that contribute to the construction of sex as risky and ambiguous. In addition, a social identity of Indian-ness and othering is used as a strategy to give meaning to the lack of parental responsibility with regard to sexual education. The use of social identity is seen as highlighting the importance of acknowledging the sexual values within which youth are embedded. This study concludes with possible ways to shift these constructions. For example, one of the conclusions suggests the implementation of an alternative school-based sexual education that acknowledges the sexual values in which youth are embedded. Furthermore, this acknowledgement of sexual values should take place within a holistic sex education programme that is positive about sexuality. Additionally, a reframing of youth as capable and active decision-makers in their sexual education is necessitated in order to see youth as a potential resource in HIV/AIDS prevention.
- Full Text:
- Date Issued: 2003
- Authors: Esat, Fazila
- Date: 2003
- Subjects: East Indians -- South Africa -- Attitudes , Youth -- South Africa -- Attitudes , East Indians -- South Africa -- Sexual behavior , Youth -- South Africa -- Sexual behavior , AIDS (Disease) -- Social aspects -- South Africa , HIV infections -- Social aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2970 , http://hdl.handle.net/10962/d1002479 , East Indians -- South Africa -- Attitudes , Youth -- South Africa -- Attitudes , East Indians -- South Africa -- Sexual behavior , Youth -- South Africa -- Sexual behavior , AIDS (Disease) -- Social aspects -- South Africa , HIV infections -- Social aspects -- South Africa
- Description: This study pays attention to youth of Indian-descent within the context of sexuality and identity and their role in HIV/AIDS. By gaining an understanding of this interaction between identity and sexuality, it adds to our knowledge of the social dynamics that contribute to the prevalence or lack of prevalence of HIV/AIDS within population groups. This study uses a social constructionist discourse analytic framework and aims to explore the construction of sexual knowledge by Southern African youth of Indian-descent. The findings indicate that the construction of sex is primarily one of risk and ambiguity. Additionally, the construction of sexual knowledge highlights the significance of gender differentials and the importance of agency and responsibility for sexual education. These constructions reinforce traditional educational roles that contribute to the construction of sex as risky and ambiguous. In addition, a social identity of Indian-ness and othering is used as a strategy to give meaning to the lack of parental responsibility with regard to sexual education. The use of social identity is seen as highlighting the importance of acknowledging the sexual values within which youth are embedded. This study concludes with possible ways to shift these constructions. For example, one of the conclusions suggests the implementation of an alternative school-based sexual education that acknowledges the sexual values in which youth are embedded. Furthermore, this acknowledgement of sexual values should take place within a holistic sex education programme that is positive about sexuality. Additionally, a reframing of youth as capable and active decision-makers in their sexual education is necessitated in order to see youth as a potential resource in HIV/AIDS prevention.
- Full Text:
- Date Issued: 2003
The subjective well-being and experience of life roles of white employed married mothers: a multiple case study
- Authors: Evans, Amelia
- Date: 2003
- Subjects: Married women -- Employment -- South Africa -- Psychological aspects , Working mothers -- South Africa -- Psychological aspects , Self-Actualization (Psychology)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:11019 , http://hdl.handle.net/10948/290 , Married women -- Employment -- South Africa -- Psychological aspects , Working mothers -- South Africa -- Psychological aspects , Self-Actualization (Psychology)
- Description: The number of women who choose to combine careers and traditional roles as mothers has been increasing steadily over the last number of years. As a result, the subjective wellbeing of these women has been the focus of many research projects over the last number of years. Subjective well-being has been defined in various ways by different authors. One definition describes subjective well-being as people's evaluations of their lives, which includes happiness, pleasant emotions, life satisfaction, and a relative absence of unpleasant moods and emotions. The current study, which took the form of a multiple case study, attempted to explore and describe White employed married mothers’ subjective experience of their well-being. The study also explored these women's experiences of combining the roles of employee and motherhood. The sample was obtained through the snowballing technique, and both qualitative (in-depth interviews) and quantitative techniques (two questionnaires - the Satisfaction with Life Scale and the Beck Depression Inventory) were utilized. The analysis of the data that was gathered was done by means of thematic and content analyses.
- Full Text:
- Date Issued: 2003
- Authors: Evans, Amelia
- Date: 2003
- Subjects: Married women -- Employment -- South Africa -- Psychological aspects , Working mothers -- South Africa -- Psychological aspects , Self-Actualization (Psychology)
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:11019 , http://hdl.handle.net/10948/290 , Married women -- Employment -- South Africa -- Psychological aspects , Working mothers -- South Africa -- Psychological aspects , Self-Actualization (Psychology)
- Description: The number of women who choose to combine careers and traditional roles as mothers has been increasing steadily over the last number of years. As a result, the subjective wellbeing of these women has been the focus of many research projects over the last number of years. Subjective well-being has been defined in various ways by different authors. One definition describes subjective well-being as people's evaluations of their lives, which includes happiness, pleasant emotions, life satisfaction, and a relative absence of unpleasant moods and emotions. The current study, which took the form of a multiple case study, attempted to explore and describe White employed married mothers’ subjective experience of their well-being. The study also explored these women's experiences of combining the roles of employee and motherhood. The sample was obtained through the snowballing technique, and both qualitative (in-depth interviews) and quantitative techniques (two questionnaires - the Satisfaction with Life Scale and the Beck Depression Inventory) were utilized. The analysis of the data that was gathered was done by means of thematic and content analyses.
- Full Text:
- Date Issued: 2003
South African women's literature and the ecofeminist perspective
- Authors: Ewing, Maureen Colleen
- Date: 2013-05-24
- Subjects: Ecofeminism in literature Ecology in literature Nature in literature South African fiction -- History and criticism Smith, Pauline, 1883-1959 Schreiner, Olive, 1855-1920 Matthee, Dalene, 1938-2005 Poland, Marguerite
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2287 , http://hdl.handle.net/10962/d1007808
- Description: A social-constructionist ecofeminist perspective argues that patriarchal society separates the human (or culture) from nature, which causes a false assumption that humanity possesses the right, as a superior species, to dominate nature. This perspective integrates the domination of nature with social conflicts, including but not limited to racial discrimination, gender oppression, and class hierarchies. Understanding how these various forms of oppression interrelate forms the main goal of an ecofeminist perspective. Since the nature-culture, female-male, and whitenonwhite conflicts resonate and interlock throughout South Africa's history, socialconstructionist ecofeminism is an indispensable perspective for analysing South African literature. This thesis takes a social-constructionist ecofeminist approach and applies it to four women authors that write about South African society between the years 1860-1900. This thesis includes the following authors and their works: Olive Schreiner (1855-1920) and two of her novels, The Story of an African Farm (1883) and From Man to Man (published posthumously in 1927); Pauline Smith (1882-1959) and her novel The Beadle (1926); Dalene Matthee (1938- ) and three of her novels, Circles in a Forest (1984), Fiela's Child (1986), and The Mulberry Forest (1987); and Marguerite Poland (1950- ) and one of her novels, Shades (1993). This thesis investigates two women from the time period (Schreiner and Smith) and two women from a late twentieth century perspective (Matthee and Poland) and compares how they depict the natural environment, how they construct gender, and how they interpret class and race power struggles. This thesis concludes that the social-constructionist perspective offers unique insights into these four authors. Schreiner's novels reveal her concerns about gender and racial conflicts in South Africa and her understanding of the nature-culture dichotomy as sustained by Social Darwinism. Smith offers insights into the complex power structures in a rural Afrikaans society that keep women and nonwhite races silent. Matthee writes nature as an active participant in her novels; the social and ecological conflicts emphasise the transformation of the Knysna area. Poland explores the racial tensions, gender conflicts, and environmental concerns that preceded the South African War. Schreiner, Smith, Matthee, and Poland make up a small cross-section of South African literature, but they provide a basis for further discussing the ecofeminist perspective within a South African context. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Ewing, Maureen Colleen
- Date: 2013-05-24
- Subjects: Ecofeminism in literature Ecology in literature Nature in literature South African fiction -- History and criticism Smith, Pauline, 1883-1959 Schreiner, Olive, 1855-1920 Matthee, Dalene, 1938-2005 Poland, Marguerite
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2287 , http://hdl.handle.net/10962/d1007808
- Description: A social-constructionist ecofeminist perspective argues that patriarchal society separates the human (or culture) from nature, which causes a false assumption that humanity possesses the right, as a superior species, to dominate nature. This perspective integrates the domination of nature with social conflicts, including but not limited to racial discrimination, gender oppression, and class hierarchies. Understanding how these various forms of oppression interrelate forms the main goal of an ecofeminist perspective. Since the nature-culture, female-male, and whitenonwhite conflicts resonate and interlock throughout South Africa's history, socialconstructionist ecofeminism is an indispensable perspective for analysing South African literature. This thesis takes a social-constructionist ecofeminist approach and applies it to four women authors that write about South African society between the years 1860-1900. This thesis includes the following authors and their works: Olive Schreiner (1855-1920) and two of her novels, The Story of an African Farm (1883) and From Man to Man (published posthumously in 1927); Pauline Smith (1882-1959) and her novel The Beadle (1926); Dalene Matthee (1938- ) and three of her novels, Circles in a Forest (1984), Fiela's Child (1986), and The Mulberry Forest (1987); and Marguerite Poland (1950- ) and one of her novels, Shades (1993). This thesis investigates two women from the time period (Schreiner and Smith) and two women from a late twentieth century perspective (Matthee and Poland) and compares how they depict the natural environment, how they construct gender, and how they interpret class and race power struggles. This thesis concludes that the social-constructionist perspective offers unique insights into these four authors. Schreiner's novels reveal her concerns about gender and racial conflicts in South Africa and her understanding of the nature-culture dichotomy as sustained by Social Darwinism. Smith offers insights into the complex power structures in a rural Afrikaans society that keep women and nonwhite races silent. Matthee writes nature as an active participant in her novels; the social and ecological conflicts emphasise the transformation of the Knysna area. Poland explores the racial tensions, gender conflicts, and environmental concerns that preceded the South African War. Schreiner, Smith, Matthee, and Poland make up a small cross-section of South African literature, but they provide a basis for further discussing the ecofeminist perspective within a South African context. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
Geography : the state of the discipline in South Africa (2000 - 2001)
- Fairhurst, U J, Davies, R J, Fox, Roddy C, Goldschagg, P, Ramutsindela, M, Bob, U, Khosa, M M
- Authors: Fairhurst, U J , Davies, R J , Fox, Roddy C , Goldschagg, P , Ramutsindela, M , Bob, U , Khosa, M M
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6678 , http://hdl.handle.net/10962/d1006659
- Description: The research team presents the findings of a comprehensive investigation into the status and role of Geography as an academic discipline in South Africa. The paper begins by placing the discipline in historical and epistemological context. Extensive and intensive interviews were conducted with geographers at all South African universities and, on a smaller scale, in the workplace. Information was also gleaned from an array of documents. Comments on the characteristics of university departments, general school education, the geography research environment the geographer in the workplace are given. Emerging trends, many of which relate to recent socio-political change, show that contemporary emphasis is on applied geography, specific fields of specialisation, the accommodation of Environmental Science and Environmental Management, skills training and on curriculum development with a marked vocational orientation. As geographers continue addressing national and international environmental and social issues in their professional endeavours, they are alerted to critical concerns voiced with conviction by practising geographers. In the final analysis a positive conclusion is reached and the academic merit and status of the discipline is confirmed.
- Full Text:
- Date Issued: 2003
- Authors: Fairhurst, U J , Davies, R J , Fox, Roddy C , Goldschagg, P , Ramutsindela, M , Bob, U , Khosa, M M
- Date: 2003
- Language: English
- Type: Article
- Identifier: vital:6678 , http://hdl.handle.net/10962/d1006659
- Description: The research team presents the findings of a comprehensive investigation into the status and role of Geography as an academic discipline in South Africa. The paper begins by placing the discipline in historical and epistemological context. Extensive and intensive interviews were conducted with geographers at all South African universities and, on a smaller scale, in the workplace. Information was also gleaned from an array of documents. Comments on the characteristics of university departments, general school education, the geography research environment the geographer in the workplace are given. Emerging trends, many of which relate to recent socio-political change, show that contemporary emphasis is on applied geography, specific fields of specialisation, the accommodation of Environmental Science and Environmental Management, skills training and on curriculum development with a marked vocational orientation. As geographers continue addressing national and international environmental and social issues in their professional endeavours, they are alerted to critical concerns voiced with conviction by practising geographers. In the final analysis a positive conclusion is reached and the academic merit and status of the discipline is confirmed.
- Full Text:
- Date Issued: 2003
Isolation of antigenic peptides of Cowdria ruminantium and their encoding genes using a genome-derived phage display library
- Authors: Fehrsen, Jeanni
- Date: 2003
- Subjects: Bacteriophages -- Genetics Ruminants -- Diseases Heartwater
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3920 , http://hdl.handle.net/10962/d1003979
- Description: The development of new and effective vaccines and immunodiagnostic reagents requires the characterisation of antigenically relevant proteins and their interactions with the products of the immune system. Phage display technology was investigated as a means of elucidating some of the antigenic properties of the rickettsial parasite, Cowdria ruminantium (Cowdria). Randomly fragmented gene-derived libraries have been useful in elucidating viral and other epitopes, but only limited work has been done with entire genomes. A phage display library expressing a repertoire of Cowdria peptides was constructed. It was sufficiently large to represent the organism's genome, but lacked phages displaying peptides coded for by genes containing a Pvu II restriction enzyme site, including the one coding for the major antigenic protein 1 (MAP1). This was considered advantageous since MAP1 is immunodominant and has already been well characterised. Affinity selection with antibodies against Cowdria proteins other than MAP1 allowed several antibody-reactive peptides to be isolated. These selected sequences were placed in the context of the genome by screening a lambda bacteriophage library and by comparison with Cowdria DNA sequences. Apart from showing that antigenic mimics were present in the phage display library, six open reading frames encoding putative Cowdria proteins were identified. All had similarities to, or motifs in common with, membrane proteins and are thus likely to be exposed to the host's humoral immune system. Some of the proteins identified were larger than the antigens used to elicit the antibodies used for selection, probably as a result of the presence of cross-reactive epitopes. Despite limitations experienced when extending a fragmented-gene approach for epitope location to genomes, it was possible to identify an antigenic region on MAP1 by comparison with selected mimics. In addition, binding peptide sequences were identified with two monoclonal antibodies that had been raised against non-Cowdria antigens. An epitope on the VP7 protein of bluetongue virus was identified and peptides were found that reacted with a monoclonal antibody directed against malignant catarrhal fever virus. Thus, apart from being able to identify several potentially important Cowdria epitopes and genes, the fragmented-genome library holds promise as a universal reagent for identifying useful mimics.
- Full Text:
- Date Issued: 2003
- Authors: Fehrsen, Jeanni
- Date: 2003
- Subjects: Bacteriophages -- Genetics Ruminants -- Diseases Heartwater
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3920 , http://hdl.handle.net/10962/d1003979
- Description: The development of new and effective vaccines and immunodiagnostic reagents requires the characterisation of antigenically relevant proteins and their interactions with the products of the immune system. Phage display technology was investigated as a means of elucidating some of the antigenic properties of the rickettsial parasite, Cowdria ruminantium (Cowdria). Randomly fragmented gene-derived libraries have been useful in elucidating viral and other epitopes, but only limited work has been done with entire genomes. A phage display library expressing a repertoire of Cowdria peptides was constructed. It was sufficiently large to represent the organism's genome, but lacked phages displaying peptides coded for by genes containing a Pvu II restriction enzyme site, including the one coding for the major antigenic protein 1 (MAP1). This was considered advantageous since MAP1 is immunodominant and has already been well characterised. Affinity selection with antibodies against Cowdria proteins other than MAP1 allowed several antibody-reactive peptides to be isolated. These selected sequences were placed in the context of the genome by screening a lambda bacteriophage library and by comparison with Cowdria DNA sequences. Apart from showing that antigenic mimics were present in the phage display library, six open reading frames encoding putative Cowdria proteins were identified. All had similarities to, or motifs in common with, membrane proteins and are thus likely to be exposed to the host's humoral immune system. Some of the proteins identified were larger than the antigens used to elicit the antibodies used for selection, probably as a result of the presence of cross-reactive epitopes. Despite limitations experienced when extending a fragmented-gene approach for epitope location to genomes, it was possible to identify an antigenic region on MAP1 by comparison with selected mimics. In addition, binding peptide sequences were identified with two monoclonal antibodies that had been raised against non-Cowdria antigens. An epitope on the VP7 protein of bluetongue virus was identified and peptides were found that reacted with a monoclonal antibody directed against malignant catarrhal fever virus. Thus, apart from being able to identify several potentially important Cowdria epitopes and genes, the fragmented-genome library holds promise as a universal reagent for identifying useful mimics.
- Full Text:
- Date Issued: 2003
An investigation into the relationship marketing strategies applied by Eastern Cape based travel agents
- Authors: Fisher, Gary
- Date: 2003
- Subjects: Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MTech (Marketing)
- Identifier: vital:10766 , http://hdl.handle.net/10948/251 , Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Description: Relationship marketing has grown in importance. This growth affects all industries, including the tourism, hospitality and travel industry. Of particular importance is the bond of relationship marketing between suppliers (principals) and retailers (travel agents) in the distribution channel. The research was undertaken to: determine whether Eastern Cape travel agents perceive relationship marketing as important; establish, what the key elements of relationship marketing strategies are,according to literature; determine what marketing strategies are currently applied by Eastern Cape travel agents; provide guidelines to Eastern Cape travel agents to implement or improve their relationship marketing strategies. Fifty self-administered questionnaires were distributed to the entire population of travel agents in the Eastern Cape. A total of 30 travel agents responded. The empirical findings showed that: Most Eastern Cape travel agents perceived relationship marketing as important; Travel agents realized the importance of the management of such relationship marketing strategies; Most travel agents were aware of or used retention strategies to enhance relationship marketing strategies; Travel agents realized that to survive relationship marketing should be part of their future planning; All respondents believed that to successfully implement relationship marketing, all staff, from front-line to top-management, need to be committed to the programme. In conclusion, relationship marketing is growing in importance and all role players need to acknowledge and manage this trend. However, this research has shown that there is a paradigm shift as more travel agents are moving away from the traditional marketing to relationship marketing.
- Full Text:
- Date Issued: 2003
- Authors: Fisher, Gary
- Date: 2003
- Subjects: Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MTech (Marketing)
- Identifier: vital:10766 , http://hdl.handle.net/10948/251 , Relationship marketing -- South Africa -- Eastern Cape , Travel agents -- South Africa -- Eastern Cape
- Description: Relationship marketing has grown in importance. This growth affects all industries, including the tourism, hospitality and travel industry. Of particular importance is the bond of relationship marketing between suppliers (principals) and retailers (travel agents) in the distribution channel. The research was undertaken to: determine whether Eastern Cape travel agents perceive relationship marketing as important; establish, what the key elements of relationship marketing strategies are,according to literature; determine what marketing strategies are currently applied by Eastern Cape travel agents; provide guidelines to Eastern Cape travel agents to implement or improve their relationship marketing strategies. Fifty self-administered questionnaires were distributed to the entire population of travel agents in the Eastern Cape. A total of 30 travel agents responded. The empirical findings showed that: Most Eastern Cape travel agents perceived relationship marketing as important; Travel agents realized the importance of the management of such relationship marketing strategies; Most travel agents were aware of or used retention strategies to enhance relationship marketing strategies; Travel agents realized that to survive relationship marketing should be part of their future planning; All respondents believed that to successfully implement relationship marketing, all staff, from front-line to top-management, need to be committed to the programme. In conclusion, relationship marketing is growing in importance and all role players need to acknowledge and manage this trend. However, this research has shown that there is a paradigm shift as more travel agents are moving away from the traditional marketing to relationship marketing.
- Full Text:
- Date Issued: 2003
An evaluation of the effectiveness of change management models utilised by organisations in the East Cape motor industry cluster
- Authors: Flesch, Devin Edward
- Date: 2003
- Subjects: Organizational change -- South Africa -- Eastern Cape , Motor industry -- South Africa -- Eastern Cape , Industrial management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MTech (Human Resources Management)
- Identifier: vital:10856 , http://hdl.handle.net/10948/125 , Organizational change -- South Africa -- Eastern Cape , Motor industry -- South Africa -- Eastern Cape , Industrial management -- South Africa -- Eastern Cape
- Description: There has never been an age in which change did not take place. However, the increase in the unpredictability of many factors of the 21st century means that organisations are susceptible to, and must embrace change. This results in an increase in the pace at which organisations may have to deal with change. This research study evaluates the interventions considered to bring about optimal change within organisations. In order to complete this study it was necessary to address the fundamentals of change management, tools utilised to manage change and the current theoretical models that assist organisations in managing change. The study took place within the Eastern Cape as it is regarded as the second fastest growing economy in South Africa. The researcher used the automotive cluster as the population for the study as it is the largest industry within the Eastern Cape. Based on the theoretical findings of a literature survey a questionnaire was developed and distributed to a randomly selected sample in the automotive cluster. The objective of the questionnaire was to evaluate the theory used and to develop a process model of change management. The results of the empirical study were statistically analysed and interpreted. Among the most important findings of the research was the fact that organisations in the Eastern Cape do not follow any specific theoretical model for managing change and very few organisations have a formal change management policy. Although the respondents to the survey acknowledged that it is imperative to engender an awareness of change management principles, especially among top management, the majority does not adhere to this principle because it is too time consuming! This research study highlights a number of areas where the management of change can be improved and presents a practical process model that can be adapted and used by the majority of organisations within the East Cape Motor Industry Cluster.
- Full Text:
- Date Issued: 2003
- Authors: Flesch, Devin Edward
- Date: 2003
- Subjects: Organizational change -- South Africa -- Eastern Cape , Motor industry -- South Africa -- Eastern Cape , Industrial management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MTech (Human Resources Management)
- Identifier: vital:10856 , http://hdl.handle.net/10948/125 , Organizational change -- South Africa -- Eastern Cape , Motor industry -- South Africa -- Eastern Cape , Industrial management -- South Africa -- Eastern Cape
- Description: There has never been an age in which change did not take place. However, the increase in the unpredictability of many factors of the 21st century means that organisations are susceptible to, and must embrace change. This results in an increase in the pace at which organisations may have to deal with change. This research study evaluates the interventions considered to bring about optimal change within organisations. In order to complete this study it was necessary to address the fundamentals of change management, tools utilised to manage change and the current theoretical models that assist organisations in managing change. The study took place within the Eastern Cape as it is regarded as the second fastest growing economy in South Africa. The researcher used the automotive cluster as the population for the study as it is the largest industry within the Eastern Cape. Based on the theoretical findings of a literature survey a questionnaire was developed and distributed to a randomly selected sample in the automotive cluster. The objective of the questionnaire was to evaluate the theory used and to develop a process model of change management. The results of the empirical study were statistically analysed and interpreted. Among the most important findings of the research was the fact that organisations in the Eastern Cape do not follow any specific theoretical model for managing change and very few organisations have a formal change management policy. Although the respondents to the survey acknowledged that it is imperative to engender an awareness of change management principles, especially among top management, the majority does not adhere to this principle because it is too time consuming! This research study highlights a number of areas where the management of change can be improved and presents a practical process model that can be adapted and used by the majority of organisations within the East Cape Motor Industry Cluster.
- Full Text:
- Date Issued: 2003
An mLAN Connection Management Server for Web-Based, Multi-User, Audio Device Patching
- Foss, Richard, Fujimori, J I, Klinkradt, Bradley, Bangay, Shaun
- Authors: Foss, Richard , Fujimori, J I , Klinkradt, Bradley , Bangay, Shaun
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427404 , vital:72436 , https://www.aes.org/e-lib/browse.cfm?elib=12397
- Description: A connection management server has been developed that enables connections to be made between mLAN-compatible audio devices, via a client web browser on any web-enabled device, such as a laptop or PDA. The connections can also be made across IEEE1394 bridges, and will allow for the transport of audio and music data between mLAN devices on the same or separate IEEE 1394 buses. Multiple users will be able to make and break connections via the server.
- Full Text:
- Date Issued: 2003
- Authors: Foss, Richard , Fujimori, J I , Klinkradt, Bradley , Bangay, Shaun
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427404 , vital:72436 , https://www.aes.org/e-lib/browse.cfm?elib=12397
- Description: A connection management server has been developed that enables connections to be made between mLAN-compatible audio devices, via a client web browser on any web-enabled device, such as a laptop or PDA. The connections can also be made across IEEE1394 bridges, and will allow for the transport of audio and music data between mLAN devices on the same or separate IEEE 1394 buses. Multiple users will be able to make and break connections via the server.
- Full Text:
- Date Issued: 2003
A New Connection Management Architecture for the Next Generation of mLAN
- Foss, Richard, Fujimori, J I
- Authors: Foss, Richard , Fujimori, J I
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427376 , vital:72434 , https://www.aes.org/e-lib/browse.cfm?elib=12539
- Description: mLAN is a networking technology based on the IEEE 1394 standard that allows for the transport of audio and music control data between audio devices. In the original implementation of mLAN, software within each mLAN node hosted by an audio device contained high level plug abstraction and connection management software. mLAN-B is the next generation mLAN architecture that splits the connection management function between workstation and device. The high level connection management and plug abstraction capability resides on the workstation, while a thin low level connection management capability is left on the device. This approach reduces cost and complexity on the device side and ensures that mLAN systems can be easily upgraded.
- Full Text:
- Date Issued: 2003
- Authors: Foss, Richard , Fujimori, J I
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427376 , vital:72434 , https://www.aes.org/e-lib/browse.cfm?elib=12539
- Description: mLAN is a networking technology based on the IEEE 1394 standard that allows for the transport of audio and music control data between audio devices. In the original implementation of mLAN, software within each mLAN node hosted by an audio device contained high level plug abstraction and connection management software. mLAN-B is the next generation mLAN architecture that splits the connection management function between workstation and device. The high level connection management and plug abstraction capability resides on the workstation, while a thin low level connection management capability is left on the device. This approach reduces cost and complexity on the device side and ensures that mLAN systems can be easily upgraded.
- Full Text:
- Date Issued: 2003
Between the Reds and the Greens: a geographical interpretation of the land question in South Africa
- Authors: Fox, Roddy C
- Date: 2003
- Language: English
- Type: Text
- Identifier: vital:575 , http://hdl.handle.net/10962/d1006657
- Description: This inaugural lecture is in two parts: each related to a different nexus of the four types of scholarly activity. The first reflects on my career and is an examination of how I have developed as a teacher and learner. The key scholarly activities being discovery, integration and teaching. The second part reflects on the evolution of my research interests and leads up to an examination of the land question in South Africa. The scholarly activities in this part of the lecture are discovery, integration and application.
- Full Text:
- Date Issued: 2003
- Authors: Fox, Roddy C
- Date: 2003
- Language: English
- Type: Text
- Identifier: vital:575 , http://hdl.handle.net/10962/d1006657
- Description: This inaugural lecture is in two parts: each related to a different nexus of the four types of scholarly activity. The first reflects on my career and is an examination of how I have developed as a teacher and learner. The key scholarly activities being discovery, integration and teaching. The second part reflects on the evolution of my research interests and leads up to an examination of the land question in South Africa. The scholarly activities in this part of the lecture are discovery, integration and application.
- Full Text:
- Date Issued: 2003
Using value stream mapping to identify waste in the manufacturing of automotive components at Federal Mogul
- Authors: Fry, Peter-John
- Date: 2003
- Subjects: Industrial efficiency , Production planning , Value added , Automobile industry and trade -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10865 , http://hdl.handle.net/10948/271 , Industrial efficiency , Production planning , Value added , Automobile industry and trade -- South Africa -- Eastern Cape
- Description: This research addresses the application of Value Stream Mapping in the automotive component industry. The goal of this research is to investigate how Value Stream Mapping can identify waste, and to evaluate its benefits on a specific application instance. Value Stream Mapping is used to first map the current state and then used to identify sources of waste and to identify lean tools to try eliminate this waste. The future state map is then developed with lean tools applied to it. A South African company, Federal Mogul South Africa (FMSA), has experienced the impact of globalisation and the need to become globally competitive first hand. FMSA will be used as a case study to illustrate the impact of using Value Stream Mapping as a tool for identify waste and the need for improving the performance of a company’s value stream in achieving the international goals set for the company and its supply chain.
- Full Text:
- Date Issued: 2003
- Authors: Fry, Peter-John
- Date: 2003
- Subjects: Industrial efficiency , Production planning , Value added , Automobile industry and trade -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10865 , http://hdl.handle.net/10948/271 , Industrial efficiency , Production planning , Value added , Automobile industry and trade -- South Africa -- Eastern Cape
- Description: This research addresses the application of Value Stream Mapping in the automotive component industry. The goal of this research is to investigate how Value Stream Mapping can identify waste, and to evaluate its benefits on a specific application instance. Value Stream Mapping is used to first map the current state and then used to identify sources of waste and to identify lean tools to try eliminate this waste. The future state map is then developed with lean tools applied to it. A South African company, Federal Mogul South Africa (FMSA), has experienced the impact of globalisation and the need to become globally competitive first hand. FMSA will be used as a case study to illustrate the impact of using Value Stream Mapping as a tool for identify waste and the need for improving the performance of a company’s value stream in achieving the international goals set for the company and its supply chain.
- Full Text:
- Date Issued: 2003
Development of a language policy in a rural school
- Authors: Fumba, Zamumzi Norman
- Date: 2003
- Subjects: Language policy -- South Africa Language and education -- South Africa Education, Rural -- South Africa -- Peddie Black people -- Education -- South Africa Native language and education -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1940 , http://hdl.handle.net/10962/d1007798
- Description: The study was undertaken to observe and participate in the process and development of a language policy for a rural secondary school in Peddie in the Eastern Cape. This was done in collaboration with parents, learners, and teachers. The researcher acted as a researcher, facilitator and learner in the process that Iead to the final product. Twenty four learners were selected from Grade 8 to Grade 10. These learners formed three focus groups. A questionnaire and lesson observation were used to establish what was taking place in the school with regard to language practices and preferences by learners, teachers and parents. Lesson observations were recorded by a tape recorder. Outcomes are that Xhosa is the dominant language to which the learners are exposed. They only have the exposure to 'chunks' of English in class and when they read magazines, newspapers and listening to radio and TV. Teachers code switch. This is supported by both learners and their parents. Parents want their children to improve performance by being taught in the medium of a language they understand well enough. The study shows different perceptions about the language that should be used as LOLT. Parents in the study favour English as LOLT, while parents in general favour Xhosa. This view is also held by both teachers and learners. At a conscious level when teachers and learners talk about the language to be used as LOLT, they favour English, but when they are faced with the reality of the class they are ambivalent, hence they code switch. The study finally reports on the divergent views of the parents, on one hand, and those of the teachers and the learners on the other hand. The divergence will be resolved in a workshop, part of the broader process of school language policy research, which is beyond the scope of the research reported in the thesis. The final product, in the form of the school language policy, will then be drafted for presentation to the School Governing Body (SGB) for ratification and writing up process.
- Full Text:
- Date Issued: 2003
- Authors: Fumba, Zamumzi Norman
- Date: 2003
- Subjects: Language policy -- South Africa Language and education -- South Africa Education, Rural -- South Africa -- Peddie Black people -- Education -- South Africa Native language and education -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1940 , http://hdl.handle.net/10962/d1007798
- Description: The study was undertaken to observe and participate in the process and development of a language policy for a rural secondary school in Peddie in the Eastern Cape. This was done in collaboration with parents, learners, and teachers. The researcher acted as a researcher, facilitator and learner in the process that Iead to the final product. Twenty four learners were selected from Grade 8 to Grade 10. These learners formed three focus groups. A questionnaire and lesson observation were used to establish what was taking place in the school with regard to language practices and preferences by learners, teachers and parents. Lesson observations were recorded by a tape recorder. Outcomes are that Xhosa is the dominant language to which the learners are exposed. They only have the exposure to 'chunks' of English in class and when they read magazines, newspapers and listening to radio and TV. Teachers code switch. This is supported by both learners and their parents. Parents want their children to improve performance by being taught in the medium of a language they understand well enough. The study shows different perceptions about the language that should be used as LOLT. Parents in the study favour English as LOLT, while parents in general favour Xhosa. This view is also held by both teachers and learners. At a conscious level when teachers and learners talk about the language to be used as LOLT, they favour English, but when they are faced with the reality of the class they are ambivalent, hence they code switch. The study finally reports on the divergent views of the parents, on one hand, and those of the teachers and the learners on the other hand. The divergence will be resolved in a workshop, part of the broader process of school language policy research, which is beyond the scope of the research reported in the thesis. The final product, in the form of the school language policy, will then be drafted for presentation to the School Governing Body (SGB) for ratification and writing up process.
- Full Text:
- Date Issued: 2003
A strategy for the implementation of e-business and e-commerce to achieve a competitive advantage in the textile industry
- Authors: Futcher, Shane Henry
- Date: 2003
- Subjects: Electronic commerce , Competition , Textile industry
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10886 , http://hdl.handle.net/10948/140 , Electronic commerce , Competition , Textile industry
- Description: The research problem addressed in this study was to determine what is required for e-business and e-commerce to have an impact on the Textile Industry in the Nelson Mandela Metropolitan. To achieve this objective, a literature study was done to identify what strategies were needed and how e-business and e-commerce would enhance the relationships between the organisations, suppliers and customers. An empirical study was conducted to ascertain what the managers within the Textile Industry know about e-commerce and e-business and the role they have or could play within their industry.
- Full Text:
- Date Issued: 2003
- Authors: Futcher, Shane Henry
- Date: 2003
- Subjects: Electronic commerce , Competition , Textile industry
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:10886 , http://hdl.handle.net/10948/140 , Electronic commerce , Competition , Textile industry
- Description: The research problem addressed in this study was to determine what is required for e-business and e-commerce to have an impact on the Textile Industry in the Nelson Mandela Metropolitan. To achieve this objective, a literature study was done to identify what strategies were needed and how e-business and e-commerce would enhance the relationships between the organisations, suppliers and customers. An empirical study was conducted to ascertain what the managers within the Textile Industry know about e-commerce and e-business and the role they have or could play within their industry.
- Full Text:
- Date Issued: 2003
Involuntary consent
- Authors: Futter, Dylan Brian
- Date: 2013-05-24
- Subjects: Ignorance (Theory of knowledge) Responsiblity Free will and determinism Theory (Philosophy) Social ethics Blame
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2742 , http://hdl.handle.net/10962/d1007846
- Description: In this dissertation I take exception with a widely held philosophical doctrine, according to which agents are only blameworthy for the bad actions they have chosen to bring about. My argument strategy is to present cases in which agents are blamed for involuntary actions that are not in any way connected to their culpable and voluntary choices. These failures correspond, I suggest, to occasions of culpable ignorance where agents have been negligent or careless. More specifically, I claim that violations of natural duties of respect and consideration, and certain acquired role-type duties, are blamed without any voluntary consent. If my examples are persuasive, then the point is reached where a normative principle of 'voluntary consent' does not in fact coincide with people's actual practices and 'considered judgements'. In the final sections of the dissertation, I argue against the plausibility of keeping the principle and revising our judgements. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Futter, Dylan Brian
- Date: 2013-05-24
- Subjects: Ignorance (Theory of knowledge) Responsiblity Free will and determinism Theory (Philosophy) Social ethics Blame
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2742 , http://hdl.handle.net/10962/d1007846
- Description: In this dissertation I take exception with a widely held philosophical doctrine, according to which agents are only blameworthy for the bad actions they have chosen to bring about. My argument strategy is to present cases in which agents are blamed for involuntary actions that are not in any way connected to their culpable and voluntary choices. These failures correspond, I suggest, to occasions of culpable ignorance where agents have been negligent or careless. More specifically, I claim that violations of natural duties of respect and consideration, and certain acquired role-type duties, are blamed without any voluntary consent. If my examples are persuasive, then the point is reached where a normative principle of 'voluntary consent' does not in fact coincide with people's actual practices and 'considered judgements'. In the final sections of the dissertation, I argue against the plausibility of keeping the principle and revising our judgements. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Capital E for events: ways that work: useful solutions
- Authors: Garman, Anthea
- Date: 2003
- Language: English
- Type: text , article
- Identifier: vital:38359 , http://hdl.handle.net/10520/EJC146297
- Description: You would think being located in a small town in one of the most impoverished provinces in South Africa would be a drawback for making media. But a small town is a reachable, convenient laboratory environment for student journalists - and never more so than when the National Arts Festival comes to Grahamstown during the winter vacation.
- Full Text:
- Date Issued: 2003
- Authors: Garman, Anthea
- Date: 2003
- Language: English
- Type: text , article
- Identifier: vital:38359 , http://hdl.handle.net/10520/EJC146297
- Description: You would think being located in a small town in one of the most impoverished provinces in South Africa would be a drawback for making media. But a small town is a reachable, convenient laboratory environment for student journalists - and never more so than when the National Arts Festival comes to Grahamstown during the winter vacation.
- Full Text:
- Date Issued: 2003
Africa: Media
- Authors: Garman, Anthea
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158752 , vital:40225 , https://hdl.handle.net/10520/EJC146281
- Description: Those are our twin concerns as we move into this new moment in human history which is being called the "Information Society" or the "Information Age".
- Full Text:
- Date Issued: 2003
- Authors: Garman, Anthea
- Date: 2003
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158752 , vital:40225 , https://hdl.handle.net/10520/EJC146281
- Description: Those are our twin concerns as we move into this new moment in human history which is being called the "Information Society" or the "Information Age".
- Full Text:
- Date Issued: 2003