https://vital.seals.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://vital.seals.ac.za/vital/access/manager/Repository/vital:69917 Wed 31 Jan 2024 17:25:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70172 Wed 29 Nov 2023 21:24:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70162 Wed 29 Nov 2023 19:41:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65370 Wed 26 Jul 2023 15:13:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65846 Wed 26 Jul 2023 10:19:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65367 Wed 26 Jul 2023 09:58:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65774 Wed 26 Jul 2023 09:16:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65767 Wed 26 Jul 2023 09:09:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65665 Wed 24 Jan 2024 20:14:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65664 Wed 24 Jan 2024 20:13:56 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:64760 Wed 24 Jan 2024 20:09:12 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69916 Wed 24 Jan 2024 08:13:20 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70163 Wed 24 Jan 2024 08:13:17 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69920 Wed 24 Jan 2024 08:13:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70249 Wed 24 Jan 2024 08:13:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65772 Wed 19 Jul 2023 14:31:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65761 Wed 12 Jul 2023 17:33:56 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65760 Wed 12 Jul 2023 17:20:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65724 Wed 12 Jul 2023 09:37:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65723 Wed 12 Jul 2023 09:31:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65721 Wed 12 Jul 2023 09:19:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65720 Wed 12 Jul 2023 09:16:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65719 Wed 12 Jul 2023 09:10:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:61101 Wed 08 Feb 2023 13:07:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:61139 Wed 08 Feb 2023 13:07:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:61088 Wed 08 Feb 2023 13:07:25 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:61140 Wed 08 Feb 2023 13:07:25 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:61100 Wed 08 Feb 2023 08:41:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:64749 Wed 07 Jun 2023 11:29:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:64770 Wed 07 Jun 2023 08:48:12 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70519 Wed 07 Feb 2024 08:12:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70250 Wed 06 Dec 2023 17:40:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70257 Wed 06 Dec 2023 17:33:20 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65777 Tue 30 Jan 2024 21:24:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65808 Tue 30 Jan 2024 21:24:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65790 Tue 25 Jul 2023 16:09:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69913 Tue 21 Nov 2023 20:15:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:49968 100% inhibition in concentrations ≥ 30 μg/mL and TZDD1, 2 and 4 exhibited ≥ 50% inhibition activity in all the concentrations (10, 20, 30, 40 and 50 μg/mL) in the α-amylase inhibition assay. Similarly, in the α-glucosidase inhibition assay, all the four derivatives exhibited a concentration dependent activity with TZDD3 showing the most activity. All the four derivatives exhibited ≥ 30% inhibition in the aldose reductase inhibition assay except TZDD1 at 10 μg/mL. TZDD4 exhibited a concentration dependent inhibition activity in the protein tyrosine phosphatase-1B inhibition assay. Interestingly, TZDD3 showed a decreasing inhibition activity as its concentration increased from 10 μg/mL through to 50 μg/mL. In the dipeptidyl peptidase–4 inhibition assay, TZDD2 and TZDD4 exhibited ≥ 20% inhibition activity across all the concentrations and in the acetylcholinesterase assay, TZDD1, 3 and 4 exhibited ≥ 25% across all the concentrations. Interestingly, in the matrix metalloproteinase-1 inhibition assay, some of these derivatives exhibited partial activation activity and partial inhibition with TZDD1 showing activation in concentrations 10 and 20 μg/mL and inhibition in concentrations 30, 40 and 50 μg/mL. TZDD4 showed activation in all the concentrations. In the β-amyloid aggregation assay, all the four derivatives showed inhibition activity ≥ 10% except TZDD1 at 50 μg/mL. Conclusions Diabetes mellitus and Alzheimer’s disease are a type of pathology of global concern, and several researchers worldwide have strived to search for novel therapeutic treatments and prevention for diabetes as well as Alzheimer’s disease. Recent studies provide a direct link v between diabetes mellitus and Alzheimer’s disease, and the need to find novel drugs that can mitigate these two is of increasing interest. In our search for antidiabetic and anti-Alzheimer’s activity, TZD derivatives (TZDD1, TZDD2, TZDD3 and TZDD4) exhibited good antioxidant activity, anti-hyperglycaemic activity and a relatively promising anti-Alzheimer’s activity. This was observed from the in vitro evaluation performed which included α – amylase, α – glucosidase, aldose reductase, PTP1B, DPP4, amyloid β aggregation, and AChE inhibition assays. Furthermore, docking of the derivatives against PPARγ predicted a similar molecular interaction to that of thiazolidinediones using Rosiglitazone as the standard drug. Furthermore, in silico ADME profiling predicted the derivatives to have moderate to good solubility in the GI (good GI bioavailability), and also exhibited excellent drug likeness. However, they are predicted not permeate the BBB. Further in silico studies and in vivo should be conducted to establish toxicities, as well as drug delivery to the brain for effective therapeutic effect against Alzheimer’s disease.]]> Tue 21 Nov 2023 19:57:12 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65378 Tue 16 Jan 2024 14:38:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65376 Tue 16 Jan 2024 14:30:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65377 Tue 16 Jan 2024 14:30:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70121 Tue 05 Dec 2023 17:47:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70219 Tue 05 Dec 2023 14:03:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70164 Thu 25 Jan 2024 13:28:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65805 Thu 20 Jul 2023 13:00:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65845 Thu 20 Jul 2023 11:57:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65804 S0 excitation energies. The small deviation observed between the calculated and experimental spectra arises from the solvent effect. The excitation energies observed in these UV-Vis spectra mostly originated from electron promotion between the highest occupied molecular orbital (HOMO) for the less intense band and the HOMO-1 for the most intense band of the ground states to the lower unoccupied molecular orbital (LUMO) of the excited states.]]> Thu 13 Jul 2023 15:22:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65803 Thu 13 Jul 2023 15:14:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65780 Thu 13 Jul 2023 11:50:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65779 Thu 13 Jul 2023 11:36:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65776 Thu 13 Jul 2023 10:47:08 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65775 100 kDa through ultracentrifugation. Mass spectrometry also detected the most abundant peak for all fucoidans to be around 700 Da (m/z). Extracted fucoidans inhibited the activity of α-glucosidase more strongly than the commercial anti-diabetic agent acarbose but were inactive on α-amylase. Fucoidans were also shown to be mixed inhibitors of α-glucosidase. Compellingly, fucoidans synergistically inhibited α-glucosidase in combination with the anti-diabetic agent acarbose, highlighting prospects for combination therapy. Finally, fucoidans demonstrated some anti-proliferative characteristics on HCT116 cancer cells by inhibiting their ability to adhere to the tissue culture plate matrix. Furthermore, some fucoidan extracts inhibited the migration of HCT116 cancer cells from 3D spheroids. Some of our fucoidan extracts also inhibited HCT116 colony formation, demonstrating inhibition of long-term cell survival. The E. maxima water extract also inhibited glucose uptake by HCT116 cells, thereby influencing the glycolytic flux. In conclusion, biologically active fucoidans were successfully extracted from South African brown seaweeds. These fucoidans demonstrated anti-diabetic and anti-cancer properties, revealing their relevance as potential drugs for these diseases.]]> Thu 11 Jan 2024 18:08:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:64756 Thu 11 Jan 2024 18:00:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:64751 Thu 08 Jun 2023 08:48:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70513 Thu 08 Feb 2024 08:26:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70308 Thu 07 Mar 2024 18:15:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70510 Thu 07 Mar 2024 09:43:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70311 Thu 07 Mar 2024 09:43:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69921 Thu 07 Dec 2023 19:04:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65372 Thu 04 Jan 2024 11:00:35 SAST 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https://vital.seals.ac.za/vital/access/manager/Repository/vital:49972 Sun 02 Jul 2023 12:24:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70157 Sat 23 Dec 2023 17:40:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71953 Sat 06 Apr 2024 12:11:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70259 Mon 29 Jan 2024 11:42:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70258 Mon 29 Jan 2024 11:36:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70256 Mon 29 Jan 2024 11:36:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70165 Mon 29 Jan 2024 08:47:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65366 Mon 24 Jul 2023 15:34:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:65365 Mon 24 Jul 2023 15:06:34 SAST ]]> 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