https://vital.seals.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://vital.seals.ac.za/vital/access/manager/Repository/vital:3910 Wed 21 Jul 2021 13:46:32 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4032 Wed 05 Apr 2023 07:00:15 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:45103 Tue 09 Nov 2021 12:03:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:20319 Thu 16 Mar 2023 12:59:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3879 Thu 13 May 2021 16:00:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4084 Thu 13 May 2021 15:33:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4040 Thu 13 May 2021 14:22:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3949 Thu 13 May 2021 13:31:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3927 3 h), depolymerizing the pectin backbone while releasing the inulins from within the chicory roots. Analysis of various mixtures of FOS by mass spectrometry, HPLC and ¹H-NMR was undertaken. Results indicated that MS with electrospray ionization and ¹H-NMR are capable of providing relative quantitative data of the FOS present in the mixtures. The pharmaceutical effects of various sc-FOS (0.5%, v/v) and SCFA (0.3%, v/v) on certain bacterial enzymes (β-glucuronidase, urease and β-glucosidase) associated with the formation of carcinogens were also studied. These enzyme activities were not directly influenced by the sc-FOS, but were found to be remarkably decreased by SCFA, pointing toward the prebiotic effect of FOS in intestinal microflora modulation.]]> Thu 13 May 2021 11:50:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4097 Thu 13 May 2021 11:07:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4105 Thu 13 May 2021 10:52:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4136 Thu 13 May 2021 10:28:15 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3939 Thu 13 May 2021 08:52:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3884 Thu 13 May 2021 08:52:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:31489 Thu 13 May 2021 08:49:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3886 20% of its initial activity after 9 consecutive reuse cycles. When exposed to fixed concentrations of carbofuran or demeton-S-methyl sulfone, immobilised acetylcholinesterase showed similar inhibition characteristics to that of the free enzyme. The decrease in enzyme activity observed after immobilisation to the nanofibres may have been due to several reasons which include some enzyme molecules being immobilised in structural conformations which reduced substrate access to the catalytic site, participation of the catalytic residues in immobilisation and enzyme denaturation due to the reaction conditions used for acetylcholinesterase immobilisation. Similar observations have been widely reported in literature and this is one of the major drawbacks of enzyme immobilisation. In conclusion, nylon-6:chitosan electrospun nanofibres were shown to be suitable supports for facile acetylcholinesterase immobilisation and the immobilised enzyme has potential for use in pesticide detection. Future recommendations for this study include a comparative study of the GA cross-linking method for AChE immobilisation which will lead to more intensely bound enzyme molecules to prevent non-specific binding. An investigation into the effect of inhibitors on stored immobilised AChE, as well as reactivation and reuse studies, may also be useful for determining the cost-effectiveness of reusing immobilised AChE for pesticide detection in environmental water samples. Several models have been designed for the determination of the kinetic parameters for immobilised enzymes. These take into account the mass transfer resistance as well as the overall charge of the immobilisation matrix. The use of these models to analyse experimental data will give a clear understanding of the effects of immobilisation on enzyme activity]]> Thu 13 May 2021 08:19:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4023 Thu 13 May 2021 08:13:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3986 Thu 13 May 2021 08:03:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4010 Thu 13 May 2021 07:34:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3984 Thu 13 May 2021 07:32:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4104 Thu 13 May 2021 07:27:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4021 Thu 13 May 2021 07:22:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4113 Thu 13 May 2021 07:20:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3929 Thu 13 May 2021 07:14:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4049 Thu 13 May 2021 07:11:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4054 Thu 13 May 2021 07:09:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4024 Thu 13 May 2021 07:08:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4116 Thu 13 May 2021 07:05:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3919 Thu 13 May 2021 07:05:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3957 Thu 13 May 2021 07:02:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3983 Thu 13 May 2021 07:02:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3959 Thu 13 May 2021 07:01:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4133 Thu 13 May 2021 06:54:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4171 Thu 13 May 2021 06:53:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4046 Thu 13 May 2021 06:51:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4019 Thu 13 May 2021 06:49:55 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4129 Thu 13 May 2021 06:49:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4159 Thu 13 May 2021 06:40:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4073 Thu 13 May 2021 06:37:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3968 Thu 13 May 2021 06:31:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3989 Thu 13 May 2021 06:29:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4062 Thu 13 May 2021 06:26:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4020 Thu 13 May 2021 06:21:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4052 Thu 13 May 2021 06:17:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3938 Thu 13 May 2021 06:15:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3998 Thu 13 May 2021 06:13:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3965 1 mM resulted in the precipitation of protein-nanoparticle bioconjugates, due to unfavourable acidic conditions. This demonstrated that the nanoparticles were binding to and becoming stabilised by general protein in the cell-free solution. Upon addition of a sodium-bicarbonate buffer, a general increase in Pt(IV) reduction to Pt(II) was observed. The addition of the buffer also resulted in an unexplained change in particle morphology and for this reason was not used in subsequent investigations. Polyvinylpyrrolidone (PVP) was shown to compromise the reduction rate of the Pt(IV) ion by SRB cells. The presence of extracellular NP’s was suggested by the colour of the supernatant turning brown and the A334 increasing over time. Attempts to visualise the particles by transmission electron microscopy (TEM) resulted in an unexpected phenomenon where nanoparticles could be observed to form dynamically upon irradiation by the electron beam. Extended irradiation by the electron beam also resulted in structural changes of the particles occurring during observation. An increase in temperature was shown to increase the reduction rate which in turn resulted in particles decreasing in size. The starting pH was shown to have a significant effect on the reduction rate and particle morphology although specific trends could not be identified. In conclusion, the cell-soluble extract from the sulfate-reducing consortium investigated, is capable of Pt(0) nanoparticle synthesis. Precise control over the particle morphology was not attained although the mechanism was further clarified and optimal conditions for nanoparticle synthesis were determined.]]> Thu 13 May 2021 06:12:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3885 Thu 13 May 2021 06:10:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3915 Thu 13 May 2021 06:08:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3944 Thu 13 May 2021 06:01:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4027 Thu 13 May 2021 05:56:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4061 Thu 13 May 2021 05:54:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3958 Thu 13 May 2021 05:51:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4096 Thu 13 May 2021 05:48:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3897 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13 May 2021 03:15:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:45408 Mon 24 Apr 2023 13:21:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:45410 Mon 24 Apr 2023 11:43:19 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:45334 Fri 19 Nov 2021 09:15:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:45333 Fri 19 Nov 2021 08:33:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4131 Fri 10 Dec 2021 08:54:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3978 Fri 06 Aug 2021 11:41:17 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4028 Fri 06 Aug 2021 10:23:20 SAST ]]>