https://vital.seals.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://vital.seals.ac.za/vital/access/manager/Repository/vital:71911 Wed 27 Mar 2024 10:39:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73647 Wed 26 Jun 2024 11:52:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73639 Wed 26 Jun 2024 10:47:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73379 Wed 26 Jun 2024 08:46:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73506 Wed 19 Jun 2024 15:05:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73505 Wed 19 Jun 2024 14:45:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73504 Wed 19 Jun 2024 14:24:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73503 Wed 19 Jun 2024 14:10:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73489 Wed 19 Jun 2024 12:59:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73492 Wed 19 Jun 2024 12:47:20 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:70660 Wed 13 Dec 2023 11:21:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73629 Tue 25 Jun 2024 15:10:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73623 Tue 25 Jun 2024 13:55:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73622 Tue 25 Jun 2024 13:40:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73621 Tue 25 Jun 2024 13:33:25 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73620 Tue 25 Jun 2024 12:44:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73619 Tue 25 Jun 2024 12:30:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73618 Tue 25 Jun 2024 12:15:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73617 Tue 25 Jun 2024 11:55:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73615 Tue 25 Jun 2024 11:26:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73267 Tue 25 Jun 2024 10:44:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69225 Tue 24 Oct 2023 10:15:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72364 Tue 21 May 2024 13:35:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73263 Tue 11 Jun 2024 15:35:17 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73260 Tue 11 Jun 2024 15:05:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73259 Tue 11 Jun 2024 14:10:56 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73258 Tue 11 Jun 2024 13:48:52 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73248 Tue 11 Jun 2024 12:02:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73245 Tue 11 Jun 2024 11:50:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73244 Tue 11 Jun 2024 11:31:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73243 Tue 11 Jun 2024 11:08:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73241 Tue 11 Jun 2024 10:47:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73240 Tue 11 Jun 2024 10:47:39 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73233 Tue 11 Jun 2024 08:07:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72962 Tue 04 Jun 2024 13:10:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72957 Tue 04 Jun 2024 12:53:17 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72954 Tue 04 Jun 2024 12:04:32 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72941 Tue 04 Jun 2024 11:56:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72939 Tue 04 Jun 2024 11:01:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72935 Tue 04 Jun 2024 09:17:19 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72821 Thu 30 May 2024 15:19:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69295 Thu 26 Oct 2023 11:11:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72736 Thu 23 May 2024 10:26:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72728 Thu 23 May 2024 10:00:39 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72675 Thu 23 May 2024 08:54:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73566 Thu 20 Jun 2024 16:01:55 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73562 7-ONE > 8-ONE > 5-ONE which correlated exactly with results from binary guest/guest competition experiments, where 6-ONE was always preferred by H1, while 5-ONE was consistently disfavoured. Single crystal X-ray diffraction (SCXRD) analyses demonstrated that each guest compound was retained in the crystals by means of a hydrogen bond with an alcohol moiety of the host compound. Furthermore, preferred guests 6- and 7-ONE produced crystals with greater densities than guests less favoured (5- and 8-ONE). A conformational analysis of the guest geometries in the four complexes with H1 revealed that the low energy guest conformers were present. The host selectivity for 6- and 7-ONE was proposed to be due to the improved molecular packing in the crystals of the complexes containing these two guest compounds, observed from their higher crystal densities. Hirshfeld surface analyses were not useful in explaining the preference of H1 for 6-ONE relative to 7-ONE (these types of analyses were not possible for the 5-ONE and 8-ONE-containing inclusion compounds due to the nature and degree of disorder present in the guest molecules). H1 was also crystallized from γ-butyrolactone (GBL), 2-pyrrolidone (NP), N-methyl-2-pyrrolidone (NMP) and N-ethyl-2-pyrrolidone (NEP), and 1H-NMR spectroscopy revealed that all but GBL were included. The host compound was also presented with these guest solvents in various mixtures, and it was observed that NMP was an extremely favoured guest solvent, followed by NEP and NP, with GBL being consistently disfavoured in every experiment. It was therefore shown that in certain instances, H1 may serve as an alternative tool for separating some of these mixtures through host-guest chemistry strategies. The hydrogen bonding motifs present in each of the successfully formed complexes were extensively investigated through SCXRD analysis, as was the thermal behaviour of these complexes. In the latter instance, the peak temperature of the endotherm (from the DSC trace) representing the guest release was greater for the inclusion compound with favoured NMP (145.5 °C) relative to the complexes with NP (139.8 °C) and NEP (120.5 °C). Host compounds H2 and H3 were revealed to have the ability to include each of pyridine (PYR), 2-methylpyridine (2MP), 3-methylpyridine (3MP) and 4-methylpyridine (4MP). H2 displayed selective behaviour for 3MP and 4MP when presented with mixtures of these guest compounds, whilst H3 preferred PYR. In the latter case, this PYR-containing inclusion compound was also the more stable one (the guest release onset temperature was highest, Ton 66.0 °C). It was demonstrated that H2 has the ability to separate very many binary mixtures of these pyridines on a practical platform, since K (the selectivity coefficient) values were 10 or greater in many instances. However, unfortunately, the more difficult-to-separate mixtures containing 3MP and 4MP cannot be purified or separated by employing H2 and supramolecular chemistry strategies. H3 was also shown to be a likely candidate for binary guest separations in very many of the guest solutions considered here, where K was also 10 or greater, and even infinity in many cases. SCXRD demonstrated that 2MP, 3MP and 4MP were retained in the crystals of their complexes by means of classical hydrogen bonds with the host compound. Satisfyingly, this hydrogen bond between 2MP and H2 (3.0213(18) Å) was significantly longer than that between this host compound and both disorder components of 3MP (2.875(2) and 2.825(9) Å) and that between H2 and 4MP (2.8458(13) Å). This observation explains the affinity of H2 for both 3MP and 4MP, and why 2MP was disfavoured. The results of thermal experiments did not wholly concur with observations from the guest/guest competition experiments. Hirshfeld surface analyses were also conducted but were not entirely conclusive with respect to explaining the host selectivity behaviour. In the case of H3, SCXRD analyses revealed that favoured PYR experienced a classical hydrogen bond with the host compound that was statistically significantly shorter (2.795(2) Å, 165°) than those between the other guest compounds and H3. Additionally, this guest compound was the only one to be involved in a (host)C−H···π(guest) interaction (2.91 Å, 139°) and also a non-classical hydrogen bond with the host compound ((host)C−H···N−C(guest), 2.77 Å (144°)). Finally, Hirshfeld surface analyses showed also that preferred PYR experienced a greater percentage of C···H/H···C (33.1%) and H···N/N···H (11.1%) interactions compared with the complexes with 2MP, 3MP and 4MP. However, it is not clear whether these Hirshfeld observations explain the affinity of H3 for PYR.]]> Thu 20 Jun 2024 15:42:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73554 Thu 20 Jun 2024 14:46:36 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:69804 Thu 16 Nov 2023 14:23:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73367 Thu 13 Jun 2024 14:59:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73359 Thu 13 Jun 2024 11:37:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73356 Thu 13 Jun 2024 11:22:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:71827 Thu 07 Mar 2024 12:11:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72778 hawksbills > loggerheads > leatherbacks). However, sightings per unit effort (SPUE) showed that loggerheads were more prevalent than hawksbills in iSimangaliso, likely due to its proximity to this loggerhead rookery. Reefs supported mixed size aggregations with juvenile to adult-sized green turtles (straight carapace lengths, SCLs of 44.9–99.2 cm), whilst hawksbills were mostly juveniles or subadults (SCL range: 37.4–73.4 cm) and loggerheads were mostly adult-sized (SCL range: 66.9–81 cm). Adult sex ratios were slightly female-biased but not significantly different from 1:1. The longest minimum residence periods recorded for individual subadult green and hawksbill turtles (676 and 675 days respectively) and adult-sized loggerheads (621 days) were suggestive of residency. Resident sea turtle activities were typical; they wereobserved foraging, resting, cleaning and interacting year-round, whilst only loggerheads and leatherbacks were observed in breeding behaviour (e.g., patrolling and mating) just after the seasonal arrival of transient individuals. This study thus reveals that the east coast of South Africa hosts regionally important resident and transient sea turtle aggregations with some of the highest SPUE results recorded for the region. This first attempt at monitoring foraging grounds using a variety of techniques and stakeholder groups can serve as the baseline assessment for future work and the foundation for a long-term in-water monitoring programme, allowing for expansion along the entire coastline.]]> Mon 27 May 2024 12:24:52 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73614 Mon 24 Jun 2024 15:41:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73613 Mon 24 Jun 2024 14:52:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73184 Mon 10 Jun 2024 14:28:15 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73182 Mon 10 Jun 2024 13:59:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73188 Mon 10 Jun 2024 12:36:15 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73185 Mon 10 Jun 2024 11:04:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72933 Mon 03 Jun 2024 15:51:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72932 Mon 03 Jun 2024 15:36:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72931 200 m depth) are challenging to access and remain poorly studied globally. Epifaunal invertebrates comprise a critical trophic level in benthic ecosystems and can serve as indicators of the overall health and functioning. There is limited knowledge of South Africa’s offshore marine environment and benthic ecosystem functioning due to limitations in funding, resources, and the lack of suitable science-based monitoring tools which are vital for sustainable management into the future. The aim of this research was to improve the understanding of epifaunal functioning as well as their potential physical drivers in 13 South African offshore biogeographic ecotypes. The objectives of this study were threefold: 1) to investigate the functional composition of benthic epifauna from 13 biogeographic ecotypes, 2) to quantify the Functional Diversity (FD) associated with epifaunal traits between the different assemblages (i.e. biogeographic ecotypes), and 3) to evaluate the relationship between physical variables and epifaunal functional traits at the biogeographic ecotype level. Biological trait-based approaches were applied to a subset of 80 benthic epifaunal species collected from 909 offshore stations along the west and south coasts of South Africa. Nine biological traits (associated with life history, morphology and behavioural characteristics exhibited by each species) were classified into 39 modalities and weighted with biomass. Community Weighted Means (CWMs) were used to evaluate the benthic epifaunal functional trait composition of the 13 biogeographic ecotypes. Functional composition across the 13 ecotype assemblages on the west and south coast appeared to be dominated by species with similar trait compositions, such as large, long-lived, surface crawling/burrowing/filter-feeding epifauna with medium to no mobility, however, their percentage of contribution to trait expression (CWMs) was higher on the west coast. Functional diversity indices (alpha and beta) showed higher overall FD for west coast ecotypes, with most ecotypes on the continental shelf (except slopes and canyons) being significantly different (p<0.05) from ecotypes on the south coast. The global RLQ (three-table co-inertia) test did not reveal a significant relationship between biomass and physical variables or between biomass and traits (p>0.05). The fourth-corner method was used to test the significance of individual traitenvironment relationships. The fourth-corner results were similar overall to the RLQ analysis, revealing that three physical variables (temperature, depth, and fluorescence) were significantly correlated to two trait modalities belonging to the feeding mode filter-feeders (FM1) and scavengers (FM5). Scavengers were positively correlated with depth and negatively with temperature, while filter-feeders were positively correlated with fluorescence levels. This indicated that the presence of scavenging epifauna increased with a decrease in temperature and an increase in depth. While a positive significant correlation between filter-feeders and fluorescence suggested their potential tolerance or preference for environmental conditions with high levels of fluorescence. This research was the first such study to explore the functional composition and diversity of benthic epifauna and their relationship with potential physical drivers in offshore west and south coast benthic biogeographic ecotypes of South Africa. The application of new tools to quantify the functional diversity of epifauna as indicators of ecosystem health, and their potential physical drivers. This provided a platform on which to advance our understanding of benthic communities and the roles they play in ecosystem functioning under changing environments. Functional trait-based approaches such as those applied in this study can provide us with vital information on the relationship between biodiversity, ecosystem functioning, and physical drivers. Environmental stressors and changing climate patterns threaten to impact marine ecosystems and their functioning. Benthic epifauna are especially sensitive to changes in their environment and these fluctuations could potentially lead to the loss of certain benthic functionality, altering the thresholds these ecosystems have to response to disturbances. This undermines the stability of these ecosystems which can have ripple effects on the health of these ecosystems and their ability to provide the ecosystem services humans dependent on. Having suitable tools to track current and predict future changes will therefore be vital to inform management and conservation strategies for sustainable ocean utilisation.]]> Mon 03 Jun 2024 15:17:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72925 Mon 03 Jun 2024 14:40:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72828 Fri 31 May 2024 14:09:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72827 Fri 31 May 2024 13:48:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72826 Fri 31 May 2024 13:20:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72824 Fri 31 May 2024 10:41:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:72822 Fri 31 May 2024 10:11:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73597 Fri 21 Jun 2024 15:41:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73596 Fri 21 Jun 2024 14:52:56 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73595 Fri 21 Jun 2024 14:31:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73594 Fri 21 Jun 2024 14:09:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73593 Fri 21 Jun 2024 13:49:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73592 Fri 21 Jun 2024 13:14:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73590 Fri 21 Jun 2024 12:18:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73586 Fri 21 Jun 2024 12:04:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73589 Fri 21 Jun 2024 12:03:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73578 Fri 21 Jun 2024 10:26:35 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73571 Fri 21 Jun 2024 08:33:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73384 Fri 14 Jun 2024 11:49:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73383 200μg/mL). If the herbal extracts had inconclusive IC50 values in the three cell lines subsequent experiments were conducted using 100 μg/mL. The results showed that most of the combinations were antagonistic but, some combinations had synergistic or enhancement effects(1:3 for cisplatin with grape seed or green tea and tamoxifen with grapeseed or green tea, and 3:1 for cisplatin with green tea as well as for tamoxifen with fermented rooibos or green rooibos, and all the 1:1 combinations of 5- fluorouracil with all herbal extracts) with a combination index (CI) < 1. Grape seed and green tea were found to induce apoptosis in the three cell lines. Assays that were conducted to detect apoptosis induction showed positive staining for phosphatidylserine (PS), activated caspase 3, and reactive oxygen species (ROS), mitochondrial membrane depolarization. Analysis of the cell cycle showed two things; 1) that grape seed and green tea were apoptotic in HeLa and MCF7 cells only, and 2) the extracts of green tea and green rooibos, and the combinations of grape seed with all drug compounds arrested the cell in more than one phase of the cell cycle. The combinations of grapeseed and green tea potentially induced apoptosis in various manners but those with both rooibos extracts were unclear in all the cell lines. The results for combinations with grapeseed and green tea are promising and provide a basis for further research as combinations of chemotherapeutic drugs and herbal extracts may be effective therapeutic strategies.]]> Fri 14 Jun 2024 11:23:15 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73382 Fri 14 Jun 2024 09:29:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73085 Fri 07 Jun 2024 14:21:25 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73084 Fri 07 Jun 2024 14:09:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73082 Fri 07 Jun 2024 13:38:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73081 Fri 07 Jun 2024 13:35:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73080 Fri 07 Jun 2024 13:19:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73076 Fri 07 Jun 2024 11:57:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73074 Fri 07 Jun 2024 11:41:56 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73071 Fri 07 Jun 2024 11:29:32 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73070 0.05). Gender and the responses to who is at risk of getting infected with intestinal parasites had a statistically significant (p<0.05) relationship. Intestinal parasite prevalence from 2012 to 2020 in SBDM was 46.37% which means it was close to half of the sampled population. Males had the highest prevalence as compared to females, with a prevalence of 53.1% xvi in males and 46.9% in females. The highest disease infection was found between ages one and six years. Conclusion: The researcher concluded that the overall awareness, attitudes and practices on intestinal parasites in SBDM were poor. The intestinal parasites present in SBDM were Cytoisospora belli (35.4%) and Giardia lamblia (35.4%), Ascaris lumbricoides (17.7%), Trichuris trichiura (3%), and Taenia species (2%). The study also revealed that the prevalence of intestinal parasites was low from 2012 to 2020. Recommendations were made with an emphasis on education to increase disease prevention and control awareness.]]> Fri 07 Jun 2024 11:22:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73067 Fri 07 Jun 2024 11:11:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73065 Fri 07 Jun 2024 11:04:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73062 Fri 07 Jun 2024 11:01:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73061 Fri 07 Jun 2024 10:47:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73059 Fri 07 Jun 2024 10:40:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73057 Fri 07 Jun 2024 10:34:08 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73055 Fri 07 Jun 2024 10:32:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:73046 Fri 07 Jun 2024 09:57:51 SAST ]]>