https://vital.seals.ac.za/vital/access/manager/Index en-us 5 https://vital.seals.ac.za/vital/access/manager/Repository/vital:28265 Wed 13 Mar 2024 14:17:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:30776 Wed 13 Mar 2024 10:12:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27363 Wed 13 Mar 2024 09:16:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:20653 Wed 12 May 2021 23:43:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28586 Wed 12 May 2021 23:36:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28583 Wed 12 May 2021 23:21:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21305 Wed 12 May 2021 23:09:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:25963 Wed 12 May 2021 23:08:09 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28878 Wed 12 May 2021 22:54:56 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28424 Wed 12 May 2021 22:52:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:34319 Wed 12 May 2021 22:51:25 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28526 Wed 12 May 2021 22:49:19 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28374 Wed 12 May 2021 22:44:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28658 Wed 12 May 2021 22:17:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:39106 Wed 12 May 2021 20:21:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28285 Wed 12 May 2021 20:10:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:25548 Wed 12 May 2021 20:07:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27619 Wed 12 May 2021 19:28:19 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:25132 Wed 12 May 2021 19:24:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28871 Wed 12 May 2021 19:24:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27943 Wed 12 May 2021 19:20:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:33472 Wed 12 May 2021 19:13:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27766 Wed 12 May 2021 19:02:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29484 Wed 12 May 2021 19:00:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21299 Wed 12 May 2021 18:47:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28115 Wed 12 May 2021 18:27:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:34445 Wed 12 May 2021 18:21:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21351 Wed 12 May 2021 18:03:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29656 Wed 12 May 2021 18:01:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:33180 Wed 12 May 2021 17:47:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21051 Wed 12 May 2021 17:38:32 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28306 Wed 12 May 2021 17:24:55 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27312 Wed 12 May 2021 17:05:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27032 Wed 12 May 2021 16:50:39 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21930 Wed 12 May 2021 16:45:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:24014 Wed 12 May 2021 16:35:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27256 Wed 12 May 2021 16:35:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:39084 Wed 12 May 2021 16:32:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28696 Wed 12 May 2021 16:27:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:33488 Wed 12 May 2021 16:21:20 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28267 Wed 12 May 2021 16:18:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21324 30°C) did however increase electron transport rates. Rapid responses to hypo-osmotic shock (i.e. osmotic downshift during freshening events) by Cyanothece sp. cells also helped minimize cell rupture due to high turgor pressure. Zooplankton abundance within the St Lucia system was negatively correlated with salinity, while grazing experiments indicated that the typical estuarine zooplankton species are able to graze on Cyanothece sp. cells. Therefore, the disappearance of zooplankton at salinities above 60 must have been an important factor in the bloom persistence. Apart from the ecological factors that were at play in St Lucia during the bloom period, the persistence of the Cyanothece sp. bloom can be attributed to the robust nature of their nutrient uptake, nitrogen fixation and photosynthetic systems to maintain activity despite extreme hypersalinity levels.]]> Wed 12 May 2021 15:59:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:20651 Wed 12 May 2021 15:55:35 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:39080 Wed 12 May 2021 15:55:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:40607 0.05) and values of >0.20 were recorded in 40 percent (108/277) of all the isolates. The resistant genes ampC, blaOXA, tetA, tetM, dfr1, sul1, sul2, ermB, nptII and strA were detected in two or more Vibrio isolates while the SXT intergrase was detected only in one isolate. Seven of the 18 extreme drug resistant isolates possessed 1–3 plasmids of 1.5–10 kb range. A total of 321 presumptive Staphylococcus aureus isolates were recovered by culture out of which 202 (62.9 percent ) were identified as S. aureus by PCR. Of these, 33 (16.3 percent ) strains were mecA positive while the rest, 169 (83.7 percent ) were mecA negative. The sensitivity and specificity of MRSA detection by use of cefoxitin disc (30 μg) test was 93.9 percent and 91.7 percent , that for oxacillin screen agar test (6 μg/mL) was 81.8 percent and 92.3 percent while that of BrillianceTM MRSATM 2 agar was 87.9 percent and 94.1 percent respectively. Staphylococcal enterotoxin SEE was detected in 12.1 percent (4/33) of total strains followed by SEA 3.0 percent (1/33), and SEC 3.0 percent (1/33). SEB and SED were absent. Six of the isolates had the PVL gene, giving a prevalence of 18.2 percent . The most frequently detected SCCmec type was SCCmec type III (17/33; 51.5 percent ), closely followed by SCCmec subtype IVc (10/33; 30.3 percent ) and the least were SCCmec types I and II with only 3.0 percent (1/33) each. The rest four (4/33; 12.1 percent ) did not belong to any of the above types and were therefore considered to belong to other subtypes of SCCmec type IV not tested in the study. For antibiogram patterns, 12 (36.4 percent ) strains were resistant to doxycyline, 10 (30.3 percent ) to trimethroprim-sulfamethoxazole, 9 (27.3 percent ) to tetracycline and only 1 (3.0 percent ) strain was resistant to ciprofloxacin. Nineteen (57.6 percent ) of the MRSA strains had vancomycin MIC of ≤ 2 μg/mL, 4 (12.1 percent ) with MIC range of 4-8 μg/mL, and 10 (30.3 percent ) with values ≥ 16 μg/mL. Overall, 27 (91.percent) isolates showed simultaneous resistance to 3 or more antibiotics. The resistant genes tetM, tetA, ermB, BlaZ femA were detected in two or more strains resistant to various antimicrobials. Resistance to rifampicin was not caused by mutations in the rpoB gene. Conclusions: To the best of our knowledge, this study is the first study reporting a broad, and diverse bacteria flora of skin, gill, and gut of the South African dusky kob as well as the first study on human pathogenic vibrios, and MRSA from marine finfish especially those from recirculatory aquaculture systems. Also, our study revealed that Vibrio species with pathogenic potential are present in marine dusky kob and its environs. Dusky kob is contaminated with CA- MRSA and HA- MRSA that may cause difficult to treat infections with regular antibiotics. Some of the MRSA strains contained toxins that can cause food-borne gastroeneteritis. The present study revealed that MRSA and Vibrio species are potential reservoirs for antibiotic resistant genes, which could be disseminated to other bacterial species probably through plasmids, a public health risk.]]> Wed 12 May 2021 14:37:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:40606 Wed 12 May 2021 14:20:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:41374 Wed 12 May 2021 13:47:51 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21127 Tue 27 Jun 2023 06:22:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21251 Tue 13 Jun 2023 08:25:27 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27540 Tue 07 May 2024 11:36:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21250 Thu 29 Jun 2023 11:43:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:31345 Thu 13 May 2021 17:02:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28893 Thu 13 May 2021 15:31:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28273 Thu 13 May 2021 13:30:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27879 Thu 13 May 2021 11:04:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28894 Thu 13 May 2021 08:13:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:26387 Thu 13 May 2021 07:27:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27835 Thu 13 May 2021 07:25:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28483 Thu 13 May 2021 07:24:08 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21183 Thu 13 May 2021 07:06:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:25020 0.05; d<0.2), the magnitude of impairment in psychomotor function (p>0.05; d = 0.37), visual attention (p>0.05; d = 0.56), working memory (p>0.05; d = 0.61) and executive function (p>0.05; d = 0.58) was larger in schoolboy batters. In both samples, the simulation altered the modulation of heart rate significantly. Heart rate variability decreased linearly with time spent batting (p<0.01; d>0.8). During cognitive assessments, heart rate variability increased with time-on-task, where responses were significantly higher (p<0.05) in the last task of the battery compared to the first. Importantly, the results of the retest phase were the same as in the test phase and only two condition effects were observed; (i) heart rate (retest lower: p<0.04;d = 0.39), (ii) body mass (retest lower: p<0.03;d = 0.09). A task-related condition effect in heart rate variability (PNN30) was also observed (retest higher: p<0.03; d = not calculated). Resultantly, the test-retest reliability of phase 2 and 3 was high. The results indicate that prolonged intermittent batting at an intensity of 64-77 %HRmax impaired cognitive functioning in amateur batters. However, the cardiovascular and muscular strain induced by prolonged intermittent batting and its effects on cognitive functioning are mediated by intrinsic and extrinsic factors (age, training status, playing experience and hydration). Therefore, while prolonged batting has similar effects on cognitive functioning as acute bouts of physical activity, they do not share the same relationship. The author hypothesises that the continuous cognitive component inherent in prolonged batting mitigates the beneficial effects of physical activity, as demonstrated previously. Future research is needed to elucidate this relationship. Additionally, player experience affects the way in which batters regulate performance while batting; which also affects the rate of and magnitude of impairment during batting. Finally, the methodological limitations of this study provide direction for future research into batting.]]> Thu 13 May 2021 07:01:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28194 Thu 13 May 2021 06:39:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:22268 Thu 13 May 2021 06:28:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:39736 Thu 13 May 2021 06:02:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27810 Thu 13 May 2021 05:59:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:30764 0.05). Virulence factors are important for the pathogenesis of H. pylori. All the 48 clinical isolates were glmM (140 bp) positive and cagA was detected in 97.9 percent of the test isolates. The vacA gene was detected in all isolates but with different subtypes. The vacA allelic combination s1m1 was detected in 75 percent of the test isolates and s1m2 allelic combination was detected in 16.7 percent of the test isolates while the combination s2m2 was detected in 8.3 percent of the test isolates. None of the test isolates possessed the allelic combination s2m1. When H. pylori multiplication was monitored under acidic conditions in the presence of anti-urease active compounds, it was revealed that anti-urease active compounds in honey are capable of inhibiting the normal multiplication of H. pylori strains that are cagA positive, vacA positive and drug resistant. The GC-MS analysis showed that Fleures honey (urease I percent = 67.8 – 68.5 percent) and Gold Crest honey (urease I percent = 50.9 percent – 53.3 percent), all petroleum ether extracts had 27 and 26 volatile compounds. The hexane extract of Manuka honey (urease I percent = 50.0 – 53.2) had 43 compounds detected. The chloroform extract of Q Bee (urease I percent = 64.2 – 66.2 percent) had 13 volatile compounds detected. All the volatile compounds considered as representative samples of GC-MS analysis had a spectral matching ≥ 90 percent with the NIST11 library. However, the majority of compounds that were detected by LC-MS in representative honey extracts include vardenafil, urapidil, hydrocortisone, e.t.c which are drugs commonly used in the treatment of different ailments or infections that affect human beings. In addition, two xi drugs, sulfaquinoxaline and hydroxyquinoline which are used in veterinary medicine and antiseptic, disinfectant and pesticide applications in agricultural activities were detected in Little Bee honey. We therefore conclude that inhibition of urease has a bactericidal effect on drug resistant, cagA positive and vacA positive H. pylori strains growing under acidic environment.]]> Thu 13 May 2021 05:55:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28122 Thu 13 May 2021 05:25:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:22361 Thu 13 May 2021 05:25:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28116 Thu 13 May 2021 04:40:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:20981 Thu 13 May 2021 04:15:25 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27479 Thu 13 May 2021 04:15:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28373 Thu 13 May 2021 04:09:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28124 Thu 13 May 2021 04:00:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28258 Thu 13 May 2021 03:57:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27354 Thu 13 May 2021 02:47:00 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21185 Thu 13 May 2021 02:44:36 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28322 Thu 13 May 2021 02:36:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27876 Thu 13 May 2021 02:25:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27431 Thu 13 May 2021 02:14:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21266 Thu 13 May 2021 02:11:36 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:35015 Thu 13 May 2021 02:09:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27179 Thu 13 May 2021 02:02:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:30769 Thu 13 May 2021 01:55:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27740 Thu 13 May 2021 01:21:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:32597 Thu 13 May 2021 01:18:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29373 Thu 13 May 2021 01:12:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27803 Thu 13 May 2021 01:10:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29180 Thu 13 May 2021 01:05:55 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27303 Thu 13 May 2021 00:58:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:33250 Thu 13 May 2021 00:51:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:29092 Thu 13 May 2021 00:48:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:26024 Thu 13 May 2021 00:13:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28138 Thu 13 May 2021 00:04:32 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:27442 Thu 01 Feb 2024 09:48:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21242 Mon 12 Jun 2023 15:04:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:25406 Mon 12 Jun 2023 12:34:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21322 Mon 10 Oct 2022 14:27:59 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21285 Mon 03 Jul 2023 08:41:08 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:28280 Fri 15 Mar 2024 08:55:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:21089 Fri 07 Jul 2023 06:30:13 SAST ]]>